Method of producing liquid sterile nutrient mediums for operation with cells of mammals

FIELD: biochemistry.

SUBSTANCE: invention relates to biochemistry. Method of producing liquid sterile nutrient medium is disclosed. Method involves dissolving of dry components of nutrient medium in optimum volumes of solvent and sterilization of obtained solutions. Dissolution is performed for 5–10 minutes in ultrasonic bath with generator frequency of 37 kHz and amplitude of oscillations in range from 5 to 20 microns. Sterilizing ultrafiltration is carried out using cascade of three series-arranged membrane filters with pore size of 0.45–0.22–0.1 mcm and efficiency of filtration from 100 to 1,000 ml/min.

EFFECT: invention provides production of nutrient medium for cell biology in required volumes immediately before planned works, higher reliability and quality of operations with cells of mammals.

3 cl, 1 tbl, 1 dwg

 



 

Same patents:

FIELD: biotechnology.

SUBSTANCE: nutrient medium comprises sodium chloride, potassium chloride, magnesium sulphate, disodium hydrogen phosphate, potassium dihydrogen phosphate, calcium chloride, sodium bicarbonate, L-arginine, L-glutamine, L-tyrosine, L-tryptophan, calcium pantothenate, pyridoxal HCl, thiamine, meso-inositol, nicotinamide, riboflavin, folic acid, glucose, enzymatic hydrolyzate of muscle proteins, lactalbumin enzymatic hydrolyzate, sodium salt of benzylpenicillin with activity of 0.09×105-1.1×105, kanamycin sulphate with activity of 0.09×105-1.1×105, nystatin with activity of 6×104 to 6.5×104 IU/l, choline chloride, succinic acid and distilled water in a predetermined ratio of components.

EFFECT: invention enables to improve the quality of the product obtained.

1 tbl, 1 ex

FIELD: medicine.

SUBSTANCE: invention refers to biotechnology and medicine. What is presented is a method for differentiation of mesenchymal stem cells of fibrotic lungs into fibroblastic cells, characterised by recovering the cells self-maintaining in vitro for 60 days and having mesenchymal stem cell phenotype (CD44+, CD73+, CD90+, CD106+, CD31-, CD34-, CD45-) from the right lobe of fibrotic lungs and producing the fibroblastic cells when adding fibroblast growth factor 2 mcg/ml to the mesenchymal stem cell culture.

EFFECT: method enables producing the donor cells from the patients suffering idiopathic fibrosis from fibrotic pulmonary tissues.

3 dwg, 1 ex

FIELD: medicine.

SUBSTANCE: invention refers to biotechnology and medicine. What is presented is a method for differentiating pluripotent stem cell population recovered from recognised pluripotent stem cell lines into a cell population expressing markers specific for a pancreatic entoderm line co-expressing PDX1, NKX6.1, but not expressing CDX2 and NGN3.

EFFECT: method can be used for medical and biotechnological applications.

9 dwg, 2 tbl, 4 ex

FIELD: medicine, pharmaceutics.

SUBSTANCE: invention refers to biotechnology and represents a placental cell grown substrate-attached. The given cell is cultured in the three-dimensional (3D) culture environment, which enables cell propagation. The above environment implies culturing for at least three days with prolonged perfusion in the culture medium. The above cell shows no osteogenic differentiation in the environment and culture fluid, wherein the bone marrow cell grown substrate-attached shows osteogenic differentiation. The above cell expresses CD73, CD90, CD29, CD105 and D7-fib.

EFFECT: presented invention enables reducing lymphocyte proliferation.

8 cl, 35 dwg, 9 tbl, 8 ex

FIELD: biotechnology.

SUBSTANCE: method comprises scaling of diploid cells of line M-20 from cryobank of Institute of Poliomyelitis and Viral Encephalitis n.a. MP Chumakov of RAMS from the ampoule of the seed cell bank of 7 passage with obtaining the working cell bank of 16 passage. At that the cells of 20-33 passages, suitable for use in therapeutic and/or diagnostic purposes are produced by culturing in a nutrient medium containing 10% of human fibrinolytic active plasma (FAP), comprising platelet-derived growth factor PDGF in a concentration of from 155 to 342 pg/ml.

EFFECT: invention enables to increase proliferative activity of human fibroblast diploid cells.

2 cl, 2 tbl

FIELD: medicine.

SUBSTANCE: invention refers to cell biology, cell transplantology and tissue engineering. A method for increasing the angiogenic activity of stromal cells of the fatty tissue in tissues and organs involves recovering the stromal cells of the fatty tissue, culturing the recovered cells in the presence of tumour necrosis factor-alpha in amounts of 5 or 100 ng/ml for 24-72 hours, and transplanting into the tissues or organs.

EFFECT: invention can be used for repairing the injured tissues and arresting an ischemia-related developing pathology.

3 cl, 11 dwg

FIELD: medicine.

SUBSTANCE: invention relates to biotechnology, cell technologies and tissue surgery. A method for preparing a smooth muscle cell culture consists in cutting a blood vessel fragment, grinding it to a piece size of no more than 2 mm in any dimension, and incubating the pieces in a culture flask having its bottom preliminarily scratched and containing a culture medium containing 10% embyo foetal serum for at least ten days, but no more than 24 days at 37°C in the CO2 incubator environment; the method differs by the fact that the above blood vessel fragment is an ascending thoracic aorta fragment cut during the coronary artery bypass surgery; before the incubation, the above pieces of the ascending thoracic aorta fragment are kept in the culture medium containing 0.1% collagenase for at least 30 minutes, but no more than 60 minutes at 37°C and then washed in the cell culture medium.

EFFECT: invention enables preparing the cells of the aortic tissue directly from the patient's vital tissues for transplantology applications.

3 dwg

FIELD: medicine, pharmaceutics.

SUBSTANCE: present invention concerns a method for propagation and differentiation of pluripotent cells. The described method involves the stages as follows: culturing the pluripotent cells and processing the pluripotent cells with GSK-3B inhibitor, wherein the above inhibitor represents 3-[1-(2-hydroxyethyl)-1H-indol-3-yl]-4-(1-pyridin-3-yl-1H-indol-3-yl)-pyrrole-2,5-dione.

EFFECT: presented invention enables preparing an adequate amount of the cell material with maintaining an ability for differentiation to be further used for clinical applications.

10 cl, 15 dwg, 11 tbl, 11 ex

FIELD: medicine.

SUBSTANCE: invention refers to genetic engineering, tissue technologies and medicine. The method for producing the mesenchymal stem cells of pluripotent lines of the human stem cells involves producing the embryoid bodies of the human pluripotent stem cells, attaching the embryoid bodies to a Petri dish to induce spontaneous differentiation of the embryoid bodies into the mesenchymal stem cells, culturing and proliferating the mesenchymal stem cells with maintaining the identity of the mesenchymal stem cells, wherein the induction of spontaneous differentiation at the stage is performed by looping an autologous cytokine with no outer cytokine added; there are also produced the respective cells, using them, a kit and a culture method.

EFFECT: invention can be used for producing the mesenchymal stem cells in regenerative medicine and cell therapy.

15 cl, 11 dwg, 3 ex

FIELD: medicine.

SUBSTANCE: invention refers to molecular biology, biochemistry and medicine. What is presented is a composition for inducing stem cell migration in fatty tissue of adults which contains human mesenchymal stem cells from a fatty tissue of adults in an amount of 1x107 to 1x1010 as an active ingredient which express a chemokine or growth factor receptor on a cell surface, or a secretory product of the above stem cells contain the chemokine or growth factor receptor; wherein the secretory product of the stem cells of the fatty tissue of adults represents adiponectin; and wherein the human stem cells of the fatty tissue of adults are first treated with a mixture containing chemokine or growth factor.

EFFECT: invention can be used in medicine as a cell therapeutic agent for more effective stem cell migration to an affected area.

8 cl, 9 dwg, 8 tbl, 8 ex

FIELD: process engineering.

SUBSTANCE: invention relates to mixing of fluids and can be used for processing of fluids, particularly: for dispersion, emulsification and homogenization. Proposed reactor comprises tube-like working chamber with ultrasound converters secured at tube lengthwise axis and in tube perimeter and acoustically connected therewith. Tube features a square in cross-section, square side length being equal to λ, where λ is ultrasound oscillation wavelength in processed fluid. Said ultrasound converters are identical and grouped in sets of four and secured at intersections of n lines of working chamber perimeter, working chamber wall mid lines being parallel with its lengthwise axis, where n makes 1, 2, 3, …. Said converters secured at working chamber opposite walls are connected in counter phase while they are connected in preset order on mid lines of chamber walls.

EFFECT: higher efficiency of process fluid processing.

7 dwg

FIELD: physics.

SUBSTANCE: method includes placing liquid media and objects located in a medium inside a mechanical oscillatory channel system, having a nonlinear relationship between resonant oscillation frequency and amplitude, wherein the method includes maximum alignment of resonance curves of an ultrasonic vibration generator and the nonlinear resonance curve of the channel system itself by determining the nonlinear resonance curve of the channel system as the relationship between the amplitude of mechanical vibrations and frequency, determining the difference between the frequency of the generator and the resonant frequency of the channel system at the required amplitude of vibrations and, based on said difference, changing the resonant frequency of the channel system by changing the geometric dimensions of the sizes. If the difference in frequency exceeds about 1.5-2.0 times the width of the resonance curve of the generator, vibrations are generated at two or more different frequencies.

EFFECT: high efficiency of cavitational effect on a processed liquid medium and objects in the medium.

12 dwg, 1 tbl

FIELD: medicine.

SUBSTANCE: group of inventions refers to medicine, namely to molecular diagnostics. A device for exposing a liquid sample to acoustic energy by generating cavitation in a liquid sample comprises a high-intensity ultrasonic wave source and a cartridge comprising a liquid sample and a liquid-air interface. The device is configured to focus high-intensity ultrasonic waves so that the cartridge integrated into the device generates a liquid well head above the liquid-air interface inside the cartridge, while a well head drop is a nucleation element. The group of inventions also refers to a system and a method for exposing the liquid sample to acoustic energy, as well as to a machine-readable medium with a software program for implementing the above method.

EFFECT: group of inventions provides probe processing by focused acoustic energy to generate the homogenous cavitation in the sample with reducing the desired energy and power.

9 cl, 9 dwg

FIELD: physics.

SUBSTANCE: method includes placing liquid media and objects located in a medium inside a mechanical oscillatory channel system, having a natural oscillation frequency, in which parametric resonances are excited or parametric excitation of self-oscillation is performed, setting as the cavitational processing efficiency criterion the oscillation amplitude of the channel system, determining optimum frequency of oscillation frequency of power exciters with experimentally predetermined natural and parametric oscillation frequencies.

EFFECT: shorter processing time, higher power of acoustic waves and higher efficiency of cavitational processing of liquid media and objects located in the media.

12 dwg

FIELD: engines and pumps.

SUBSTANCE: invention proposes an ultrasonic mixer of vegetable oil and mineral fuel, which includes ultrasonic emitter (1) and electronic control unit (3). Ultrasonic emitter (1) is arranged in a cavity of mixer housing (7) having inlet channels (8 and 9). Voltage of an on-board network of automotive equipment (+12 V) is supplied to electronic control unit (3) that shapes and supplies high-frequency signals to the ultrasonic emitter. Vegetable oil and mineral fuel are supplied through inlet channels (8 and 9) to the mixer and mixed under action of ultrasonic vibrations.

EFFECT: treatment of vegetable and mineral components of a mixed fuel by an ultrasound leads to quality mixing and obtainment of a homogeneous fine emulsion.

3 cl, 2 dwg

FIELD: power industry.

SUBSTANCE: heat-mass-energy exchange method consists in formation of vortex annular flows of media, their direction parallel to each other with provision of partial contact of opposite directed external surface layers in radial and tangential directions to the depth providing their acoustical excitation due to deformation-shear interaction, and further combination of excited flows. One of the vortex annular flows is additionally directed in an opposite direction to the rest ones along their axes. A heat-mass-energy exchange device containing two pipes with tangential inlets, which are interconnected with each other by partial intersection along generatrixes, and an acoustical chamber; with that, tangential inlets and the outlet of one of the pipes are located in an opposite direction in relation to the tangential inlets and outlets of the rest pipes.

EFFECT: invention provides for additional excitation of flows in the zone of their contact and increase of heat-mass-energy exchange intensity.

2 cl, 6 dwg

Amber varnish // 2543169

FIELD: chemistry.

SUBSTANCE: amber varnish includes milled amber, linseed-oil, if necessary, white-spirit and/or turpentine. After the dissolution of milled to 6 mcm amber in hot linseed-oil and thermal processing at 120-160°C for 40-80 min, filtering, and, if necessary, dilution with white-spirit and/or turpentine, ultrasonic processing of the formed suspension with the power density of 0.1-10 W/cm3 and frequency of 22-35 kHz is carried out.

EFFECT: acceleration of amber dissolution and increase of its content in the final product.

1 dwg, 3 tbl, 10 ex

FIELD: process engineering.

SUBSTANCE: invention relates to material crushing means. Proposed method implemented in this device comprises the step that follow. Material is mixed with water and placed into dispersion chamber. The latter is sealed to feed 5-30 atm of static pressure therein. Contents of said chamber is processes with ultrasound vibrations at insonation density of at least 50 W/cm2 to bring sound pressure to said mix of material with water that is higher than said static pressure 2 to 3 times.

EFFECT: homogeneous particles in the range of tens of nm.

10 cl, 2 dwg

FIELD: chemistry.

SUBSTANCE: mechanically crushed powder of metal/carbon nanocomposite, which represents nanoparticles of 3d metal, such as copper or nickel, or iron, stabilised in carbon nanofilm structures, are mechanically ground together with introduced in portions organic compound in ratio 3:1. Obtained mixture is dispersed by means of ultrasound within time, corresponding to maximal ratio of peak intensities on IR-spectrum with equal wave numbers of obtained suspension and organic compound. As organic media used are ethyl alcohol, toluene, acetone, isomethyltetrahydrophthalic anhydride, mixtures of organic substances.

EFFECT: obtaining suspension based on organic compound and nanocomposite with regulated activity, controlled by method of IR-spectroscopy.

8 cl, 17 tbl

Vibration mixer // 2516569

FIELD: construction.

SUBSTANCE: vibration mixer comprises a mixing chamber with windows of materials loading and unloading, a rotor with a rotation drive made with blades. In the lower and upper parts of the mixing chamber in the centre there are two: lower and upper vibrators, made in bodies with excitation of oscillations by means of lower and upper crank-and-rod mechanisms, accordingly, and with a function of development of effect of superimposition of vibration fields in the centre of the mixing chamber from the lower and upper vibrators. Bodies of vibrators are made in the form of identical metal corrugated shells, which represent corrugated thin-walled solids of revolution and made as capable of developing two vibration fields that are identical in amplitude and different in frequency, each corresponding in shape to a corrugated contour of these solids of revolution, with differently directed oscillations. Inside each shell in the centre in the horizontal plane there are discs fixed rigidly with guide stands, the function of which is to create stable directed reciprocal movements of stems from reciprocal pairs. Discs of lower and upper vibrators are made with cylindrical protrusions, the function of which is to insert and fix springs along the inner diameter, and springs are installed with a function of free compression/release in sleeves, to the centres of inner parts of which there are stems fixed rigidly, to the centre of outer parts there are pushers fixed, which are rigidly fixed by the other end to inner parts, forming least of corrugations of metal corrugated shells, and made with a function of excitation of oscillations from least of corrugations of the vibrator bodies. At the same time at the external cylindrical parts of the sleeves there are four ledges fixed symmetrically on each one, and their function is to transfer reciprocal movements to discs, made as capable of homogeneous distribution of vibration fields from least of corrugations of metal corrugated shells to areas of fixation of bodies by means of creation of homogeneous amplitude values of movements of each point of external forming metal corrugated shells at the moment of compression of springs until stop at rubber gaskets, made with a function of softening of ledges collision with discs.

EFFECT: expanded arsenal of technical facilities, increased intensity of a process of mixture components mixing, increased efficiency of a vibration mixer.

3 dwg

FIELD: machine building.

SUBSTANCE: mixer of bulk materials containing loading and unloading devices, fixed casing, in it a shaft with spraying nozzle is installed, the nozzle is coaxial cone chambers with the connected to them internal and external spraying channels located coaxially. The spraying channels are of different length, at that the internal channels have larger length, and in casing the baffle is installed made out of two coaxial rings, two which two rows of the elastic material are connected with clearance, the rows are in form of the cone surface.

EFFECT: invention improves efficiency of mixing.

2 dwg

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