Granule for use in powder detergents and composition of granule detergent
SUBSTANCE: granule for use in powder detergents, and composition of granule detergent are suggested. The granule contains core and protective coating in amount of 10-50 wt % relatively to the core. The core contains amylase, protease, cellulase or lipase, thiosulphate of alkali or alkali-earth metal or methionine - 0.5-5 wt % in relation to the core, magnesium sulphate or zinc sulphate - 0.1-15 wt % of waterless salt in relation to the core, and mixture of citric acid and citrate - 1-5 wt % in relation to the core. The protective coating contains at least 75 wt % of sodium sulphate, and one or more other materials selected from the fillers, substances preventing their adhesion, pigments, dyes, plastifiers, binding substances. Composition of granule detergent contains the surface-active substance and said granule.
EFFECT: due to synergistic effect the invention granule components improve stability of the ferment during storage, when the granules are added to detergent composition containing the corrosive components.
7 cl, 6 ex
SUBSTANCE: invention relates to biotechnology and specifically to novel solid enzyme compositions containing a mixture of at least one salt-stabilised enzyme composition, least one carrier in form of particles and at least one hydrophobic liquid. The invention also relates to a method of preparing said solid enzyme compositions, as well as animal feed, food products and food additives containing such enzyme compositions.
EFFECT: invention enables uniform batching to food products and feedstuff, and also ensures high demixing stability, extremely low susceptibility to dust and excellent fluidity of said compositions.
41 cl, 2 dwg, 2 ex
SUBSTANCE: digestive agent represents a complex enzyme preparation containing protease produced by Bacillus licheniformis bacteria, alpha amylase produced by Bacillus amyloliquefaciens bacteria and lipase produced by Yarrowia lipolytica yeast. The ration (activity unit) is specified to be equal to 0.055:0.85:1 respectively.
EFFECT: extended range of digestive agents of high pharmacological activity of microbial enzymes.
6 tbl, 8 ex
FIELD: food processing industry, biotechnology.
SUBSTANCE: invention relates to polypeptide having xylanase activity which is capable to decompose of cellulose extracts and plant materials. In another embodiment disclosed is polynucleotide encoding said polypeptide, as well as composition containing polypeptide of xylanase activity. Abovementioned polypeptide is useful in treatment of plant or xylan-containing material for preparation of bread and animal feedstuff.
EFFECT: enhanced assortment of xylanases useful in industry.
23 cl, 10 tbl, 12 ex
FIELD: biotechnology, feedstuff production.
SUBSTANCE: method for phytase-containing aqueous liquid includes cultivation of genus Aspergillus microorganism transformed with expression vector, containing phytase gene of said microorganism bond to promoter and/or signal sequence of amyloglucosidase gene. Cultivation is carried out in aqueous medium containing carbon and nitrogen source under conditions, which allow recombinant phytase expression. Cultural liquid is filtered, cation exchange chromatography, anion exchange chromatography, and ultrafiltration are carried out to produce phytase-containing aqueous liquid having activity at least of 14000 U/g. Said aqueous liquid with phytase activity is useful in production of granulated material, animal feed, premix or semi-finished animal feed.
EFFECT: granulated material and animal feed for animal growth stimulation.
24 cl, 2 tbl, 10 ex
FIELD: medicine, pharmaceutics.
SUBSTANCE: claimed invention relates to method of supplying versions of protease or amylase with efficiency value in, at least one test of interest, exceeding index of efficiency for said enzyme of wild type. Multiple enzyme versions, encompassing certain range of the total surface charge or electrokinetic potential in, at least, one test of interest, are tested. Such test includes measurement of: efficiency of washing, substrate binding, enzyme inhibition; levels of expression, stability in detergent and/or thermal activity. Value of surface charge or electrokinetic potential, at which efficiency in test constitutes at least 50% of the maximal one, is identified. New set (library) of enzyme variants, enriched with members, possessing values of at least 50% of the maximal value, in said, at least, one test of interest within the limits of said charge or potential value. Said set of enzyme variants and, at least, one enzyme of wild type are tested in said, at least, one test of interest. If value of efficiency index is higher than index for wild type enzyme, enzyme variants, demonstrating improved result, are identified.
EFFECT: application of invention provides novel method of obtaining variants of enzymes with improved activity.
19 cl, 32 dwg, 14 tbl, 15 ex
SUBSTANCE: invention relates to a metal-salen complex derivative. The complex is represented as A-B-C, where A is the metal-salen complex, B is a bond zone, including at least one disulphide bond; and C is a functional molecule, consisting of at least one of enzymes, antibodies, antigens, peptides, amino acids, oliginucleotides, proteins, nucleic acids and medication molecules. The bond zone (B) includes a molecule of a cross-linking agent to form a cross-linking between the said metal-salen complex (A) and the said functional molecule (C). The said metal-salen complex (A) and the said molecule of the cross-linking agent are bound together via at least one disulphide bond; and the said molecule of the cross-linking agent and the said functional molecule (C) are bound together via at least one disulphide bond. The zone of the disulphide bond (B) results from the formation of a bond between a SH group, introduced as a substituent into the said metal-salen complex, and the SH group of the said functional molecule (C), or results from the formation of a bond between the SH group of the said metal-salen complex (A) or the said functional molecule (C) and the SH group of the cross-linking agent molecule. Also claimed is a method of obtaining the metal-salen complex derivative.
EFFECT: invention makes it possible to obtain the derivative of a metal-salen complex, characterised by an excellent output and stability.
3 cl, 2 ex
FIELD: food industry.
SUBSTANCE: invention relates to the field of food industry and represents a brewage method involving thermostable protease addition to the wort after the latter filtration but before cooking; protease thermostability means that such protease activity accounts for at least 70% of its activity measured in the following way: protease is diluted till concentration equal to 1 mg/ml in an analytic buffer (containing 100 mmol of succinic acid, 100 mmol of HEPES, 100 mmol of CHES, 100 mmol of CABS, 1 mmol of CaCl2, 150 mmol of KCl, 0.01% Triton X-100) with pH conditioned to 5.5 with the help of NaOH; the protease is pre-incubated i) in ice and ii) for 10 minutes at a temperature of 70°C; the substrate in relation whereto protease displays activity is suspended in 0.01% Triton X-100: for reaction beginning protease is added in an amount of 20 mcl into a test tube and incubated in an Eppendorf thermomixer at 70°C, 1400 rpm during 15 minutes; the reaction is stopped by way of the test tubes placement into ice; the samples are centrifuged in a cold condition at 14000 g during 3 minutes; the supernatant optic density OD590 is measured; the obtained OD590 value of samples without protease is subtracted from the obtained OD590 value of samples treated with protease; protease thermal stability is determined by way of calculation of protease percentage activity in the samples pre-incubated at a temperature of 70°C relative to protease activity in the samples incubated in ice as 100%-activity.
EFFECT: invention allows to enhance colloidal stability of wort and beer as well as preserve the level of total nitrogen in wort and beer due to protease addition to filtered wort.
16 cl, 1 dwg, 17 tbl, 7 ex
SUBSTANCE: method of production of nuclear proteinases of non-histone and histone plant proteins, in which initially in certain time intervals from the start of soaking 0 h, 3 h, 6 h, 9 h, 12 h, 15 h, 18 h, 21 h, separation of corcules from endosperm is carried out the followed by preservation of the corcules in the buffered 80-90% glycerol at -25°C, cell nuclei are isolated, the nuclear fractions are extracted with increasing concentrations of 0.14 M, 0.35 M, 2 M sodium chloride and 6 M guanidine hydrochloride with 0.1% β-mercaptoethanol, the histone and non-histone proteins are isolated from the above fractions using ion exchange chromatography with amberlite IRC-50 in the discontinuous gradient of guanidine hydrochloride: 6%, 8.9%, 10.6%, 13%, 40% on 0.1 M potassium-phosphate buffer pH 6.8, the resulting proteins are passed using the method of affinity chromatography through the column with sepharose 4B with immobilised trypsin inhibitor followed by evaluation of the proteolytic activity.
EFFECT: invention can be used to analyse the molecular-genetic mechanisms of formation of structures of the cell nucleus, which is necessary to obtain additional information in the development and construction of computer models of organisation of gene and epigene control networks.
FIELD: food industry.
SUBSTANCE: inventions group relates to bakery industry. The method for bakery products chewing easiness improvement involves addition of at least one medium-thermoresistant or thermoresistant serine proteinase or metalloproteinase into the said bakery product where one measures texture parameters represented by force (g) and total work function (g·sec) necessary for destruction of an identical sample of bakery products. The force and total work function are improved at least by 10% in comparison with an identical sample failing to contain at least one of the said serine proteinase or metalloproteinase. The ratio of the said serine proteinase or metalloproteinase activity at the temperature optimum to the said serine proteinase or metalloproteinase activity at 25°C is equal to more than 10. Additionally, the inventions group envisages a bakery product chewing easiness improver (containing a thermoresistant metalloproteinase) where the ratio of the said thermoresistant metalloproteinase activity at the temperature optimum to the said proteinase activity at 25°C is equal to more than 10 as well as a chewing easiness improver for bakery products with a crust; the latter improver contains 1300 - 10400 units of Taq proteinase /100 kg of flour.
EFFECT: improvement of bakery products chewing easiness and texture parameters.
21 cl, 5 dwg, 12 tbl, 13 ex
SUBSTANCE: invention proposes a production method of recombinant protein through its hybrid precursor substance with natural decomposition site with enteropeptidase. The result is achieved by replacement in natural decomposition site with enteropeptidase Asp-Asp-Asp-Asp-Lys of amino-acid residue of lysine (Lys) with amino-acid residue of arginine (Arg) and further decomposition of hybrid precursor substance with light catalytic subunit of enteropeptidase of a human being or a bull.
EFFECT: improving quality and yield of target product under conditions when hybrid protein detects additional sites of decomposition with enteropeptidase.
3 tbl, 3 dwg, 4 ex
SUBSTANCE: proposed enzymatically inactive IgA1 protease with replacement Ser267Ala for use as a component of a polyvalent vaccine designed to protect people against meningococcal infection and other microorganisms, which pathogenicity is caused by IgA1 protease. The invention includes a polynucleotide encoding the said mutant form of IgA1 protease of Neisseria meningitidis of serogroup B, comprising the said polynucleotide, a recombinant plasmid DNA, the strain Escherichia coli - producer of a mutant form of IgA1 protease according to the invention, the method of preparing a recombinant form of the enzyme using a technology of recombinant DNA, and recombinant inactivated IgA1 protease.
EFFECT: increased level of immunogenicity.
6 cl, 1 tbl, 5 dwg, 7 ex
SUBSTANCE: strain Escherichia coli Russian National Collection of Industrial Microorganisms B-10996 is cultivated on a nutrient conditioned medium containing: peptone 2-4 wt %, yeast extract 1 - 2 wt % and water the rest in the presence of a selective antibiotic to stationary growth phase at temperature 20 °C to 30°C. The prepared is dissolved in a fresh portion of the same medium in the ratio 1:1 to 1:3. Simultaneously an inducer is added, and the cultivation is continued to for at least 4 hours. Synthesised proteinase is purified.
EFFECT: ensured preparation of enzymatic active proteinase U1p275 - a version of SUMO-proteinase U1p1 of yeast Saccharomyces cerevisiae which contains a catalytic domain of proteinase U1p1 and comprises a sequence of 6 histidine residues on the C-terminal.
SUBSTANCE: method involves tray coating with a bacterial antigen of any representative of an opportunistic microflora, coating of a sorbed antigen with IgG, IgM and IgA antibodies, a proteolytic reaction by incubation in tray wells of an analysed biological fluid together with inhibitors of active centres of the enzymes (serine, cysteine, metal-dependent), washing of the reaction products and calculation of enzymatic activity with appropriate type of the active centre.
EFFECT: invention provides simultaneous measurement of activity of proteinase of various origin and of various types of the active centres.
4 tbl, 4 ex
SUBSTANCE: method for producing total water-soluble proteolytic enzymes from dried milky sap of green papaya fruits provides water extraction of a raw material at room temperature and with mixing, separation of solid and liquid phases, and recovery of a purified product. An aqueous extract produced after separation of the solid and liquid phases is post-filtered through a filter of pore size (0.45-2.0) mcm. The product is dried by a spraying technique to prepare a purified product in the form of a dry extract of proteolytic activity not less than 3.5 PU/mg of the preparation and the protein content 0.43-0.50 mg/mg of the preparation. The prepared dry extract is water-dissolved that is followed by sterilisation filtration and lyophilisation of the solution.
EFFECT: method allows preparing a product of high proteolytic activity and protein content.
2 cl, 1 ex
SUBSTANCE: strain of micromycete Aspergillus foetidus 379-K-5-1 is proposed, producing a complex of pectinases, β-glucanase, xylanase, cellulase, chitinase, mannanase and protease for the destruction of polysaccharides of a plant and microbial material. The strain is deposited in the Departmental collection of useful microorganisms for agricultural purposes of the Russian Academy of Agricultural Science (RCAM) of the State Scientific Institution of the Russian National Research Institute for Agricultural Microbiology under the registration number of RCAM 01136. The advantage of the new strain is to obtain the more active complex of enzymes catalyzing the hydrolysis of polysaccharides, as well as endopolygalacturonase and pectinesterase. The strain Aspergillus foetidus RCAM 01136 is produced using efficient methods of selection and mutagenesis using UV irradiation and nitrosoguanidine from the known strain.
EFFECT: invention enables to expand the range of the enzymes obtained, used for the destruction of polysaccharides.
SUBSTANCE: invention relates to biochemistry. Described are xyloglucanase versions, which have a number of substitutions, as well as polynucleotides, coding said xyloglucanase versions. Claimed is host-cell, containing said polynucleotide. Described are methods of obtaining said versions of xylogluconases.
EFFECT: invention extends arsenal of xylogluconases.
11 cl, 51 tbl, 4 ex, 1 dwg
FIELD: medicine, pharmaceutics.
SUBSTANCE: invention relates to an enzyme composition, capable of efficient decomposing the cellulose material in an efficient way, and can be used in the production of sugars from the cellulose biomass. The composition includes a mixed cellulose composition and recombinant swollenin, produced by Trichoderma reesei. The mixed cellulose composition includes endoglucanase, cellobiohydrolases and β-glucosidase. A ratio of the mixed cellulose composition and swollenin in the claimed composition is between approximately 10:1 and approximately 1:2.
EFFECT: application of the claimed composition in cellulose decomposition provides an increase of glucose output.
5 cl, 5 dwg, 4 tbl, 5 ex
SUBSTANCE: invention relates to biotechnology. Disclosed is a detergent composition containing an isolated mutant version of a parent xyloglucanase, 2-50 wt % surfactant and an auxiliary ingredient, where the version of the parent xyloglucanase contains one of the groups of alterations presented in the description; the parent xyloglucanase is xyloglucanase having at least 90% identity to the amino acid sequence SEQ ID NO:3, presented in the description; and the version has xyloglucanase activity. Disclosed is use of said composition for endowing cotton with dirt-repellent properties during subsequent washing.
EFFECT: invention enables to obtain a detergent composition with stable xyloglucanase for endowing cotton with dirt-repellent properties during subsequent washing.
11 cl, 1 dwg, 56 tbl, 29 ex
SUBSTANCE: invention relates to field of molecular biology and biochemistry. Claimed is polynucleic acid, coding cross-linked protein to provide secretion of cell wall-cleaving enzyme, in microorganism of class Clostridia, as well as respective recombinant microorganism and method of production and secretion of said protein.
EFFECT: invention can be used for cellulolytic procedures in industry.
13 cl, 5 dwg, 5 ex
SUBSTANCE: invention relates to field of biochemistry and biotechnology. Performed are: crushing and sieving of lignocelluloses material and selection of granules with particle size from 0.08-0.1 mm. As lignocelluloses material used are: straw, grass, chip, corncobs, bagasse and oil cake. Obtained granules are mixed with weight ratio granules-water 1:(1-5) at temperature 70-90°C. They are dispersed through colloid mill for 1-2 hours to obtain suspension with particle size 40-80 mcm. Homogenisation of obtained suspension is performed under pressure 50-100 atm and temperature 60-85°C for 1-2 hours. Suspension with particles with size 10-40 mcm is obtained. Bufferisation of obtained suspension with buffer solution of sodium acetate and acetic acid with pH value = 4.8-5.8 is carried out. Mixture of enzymes: cellulose in amount 10-60 international units per gram of lignocelluloses material, β-glucosidase in amount 40-100 international units per gram of lignocelluloses material and xylanase in amount 60-120 international units per gram of lignocelluloses material is added. Mixture of enzymes is introduced into reactor after cooling suspension to the temperature of carrying out enzymolysis 40-55°C. Enzymolysis is carried out in reactor for 36-72 hours at rate of reactor rotation 80-160 revolutions per minute. Content of sugar in hydrolysate is determined by method of liquid chromatography.
EFFECT: invention makes it possible to carry out processing of lignocelluloses material at low temperature 40-55°C.
7 cl, 3 dwg, 3 ex