Method for evaluating immune functional body reserve

FIELD: medicine.

SUBSTANCE: peripheral leukocyte blood values before and after a loading test are measured with the use of the gradual submaximal exercise. The method involves the differentiated recovery of different types of leukocytes from the blood to produce a preparation; a total T-cell (Tt) and active T-cell (Ta) count is determined; a Tt/Ta ratio is derived; mononuclear cells are incubated with granulocytes; a granulocyte-binding lymphocyte index (GLI) is determined in accordance with a granulocyte rosette formation (GRL - contact bound to three granulocytes) to granulocyte contact lymphocytes (GCL - contact bound to one granulocyte) ratio in the preparation; leukocyte indices: lymphocyte index (LI), immune reactivity index (IRI), adaptation index, ("CПHP") are determined; immune functional state adaptation coefficients (K) are derived for each value. Total immune functional body state TIFBS is calculated by formula TIFBS = K"спнр"+Kli+Kiri+Kgli+K"итл" before the exercise - TIFBS1 and after the exercise - TIFBS2. A specific immune functional state coefficient SIFSC is calculated by formula SIFSC = (TIFBS1+TIFBS2)/5, and a level of the immune functional SIFSC reserve is determined. If the SIFSC values are above +1.0, the level of the immune functional reserve is considered to be optimal; the SIFSC values falling within the range of 0 to 1.0 show the satisfactory reserve, whereas the SIFSC values below 0 shows the unsatisfactory reserve.

EFFECT: method enables assessing the functional body reserves with the use of combined characteristics including the adaptation body potential, immune reactivity and immune cell interaction.

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The invention relates to medicine, namely to physiology, and can be used to characterize the immunological health.

Adaptation reserves in General are cells, tissues, organs, organ systems and organism as a whole to withstand different types of loads (Meerson F. Z. Adaptation, stress and prophylaxis. - M.: Nauka, 1981 - 278 S. Bulatova M. M. Theoretical and methodological bases for the implementation of functional reserves of athletes in training and competition activities: author. dis. d RA PED. Sciences. - K., 1996. - 50 p.). In the adaptation reserves are allocated structural (morphological) and functional resources (Mozzhukhin A. S. Physiological reserves of the athlete. - L.: GDOIFK them. Lesgaft, 1979. - 16 p.).

The difficulty of obtaining reliable technologies to assess the level of functional reserves caused by the fact that the tested physiological parameters have disparate structures and contribute unequally, ambiguously defined contribution in the research process. The concept of functional reserve is not sufficiently precise and unambiguous descriptions with clearly defined boundaries in accordance with its levels (Korostelev A. N. Methods and tools for the classification and measurement of the level of functional reserve of human rights on the basis of hybrid models. Author. Diss. Cand. tech. NAU�. Kursk, 2012. 19 p). We propose, based on the classification by A. N. Korosteleva classes of functional reserve, to allocate the following levels of immuno-functional reserve of the human body: an optimal immuno-functional reserve in healthy humans, allowing him to function at a reasonably high loads; satisfactory immuno-functional reserve, allowing it to function normally in normal conditions without significant congestion; poor immune functional reserve, in which high risks of occurrence and development of disease and / or unexpected interruption in the work due to the lack of reserve opportunities of an organism.

Known "Method of assessing adaptive capacity" (patent RU 2314019, AV 5/02; published 10.01.2008), which is based on determination of the frequency of the pulse at the radial artery for 1 minute after 15 minutes of rest with subsequent determination of the adaptation potential (AP) by the formula: AP=1,238+0,09·PE, where PE is the pulse frequency, 1,238 and 0.09 - coefficients of the equation. For different values of AP level mechanisms of adaptation assessed as satisfactory, stressful, unsatisfactory, a failure to adapt.

A method of assessing adaptive capacity in patients with breast cancer (Patent RU 2392866, AB 10/00, G01N 33/48; published 27.06.2010) based on ODA�dividing indexes of peripheral blood leukocytes: lymphocytic index index adaptation index of immunoreactivity, with the subsequent calculation of the coefficient of adaptation for each indicator and the aggregate index of adaptive capacity (surfactants) of the body, based on the extent to which adaptive capacity is estimated as a reduced, satisfactory, high.

There is a way of determining the functional state of the person (Patent RU 2289301, AV 5/0452, AV 5/021 published 22.11.2004), based on the detection of cardiac rhythm, duration of RR-intervals, the values of systolic and diastolic blood pressure with the calculation of an indicator of the functional state of human rights (SDRs) by the formula:

SDR=AMo/(2Mo×DX)×(ADs+ADd)/(ADs-ADd) where Mo is the most frequently occurring value of the duration of RR-intervals; AMO - the ratio of the number of values of RR-intervals, the duration of which is equal to Mo, the total number of registered R-R; DX - the difference between the maximum and minimum values of the duration of RR-intervals; ADs - value systolic blood pressure; ADd - value diastolic blood pressure.

A method of assessing the functional capacity during exercise, which consists in the measurement of electrocuting AC resistance supplied through 2 cutaneous electrodes, one on goal�ve surveyed. By reducing resistance in comparison with its value at the start of the load of the functional reserve of the organism is considered adequate, and the increase is inadequate (Patent RU №2012227, G01R 27/00, AB 5/05 published 15.05.1994).

Famous assessment of reactions of an organism in response to physical exercise on which to judge the functionality of the surveyed focused on physically healthy people and athletes. They use tests that require significant physical effort, for example a test with squats, Harvard step test, load Bicycle exercise test using a treadmill ("running" track), some other methods (Therapeutic physical culture: the Guide / Epifanov V. A. et al. - M.: Medicine, 1987. - 528 S.; Zhuravlev, A. I., Gruevska N. D. Sports medicine and physiotherapy. / Manual. - M.: Medicine, 1993. - 432 p.). Based on the results of the analysis of indicators of the number of heart rate, blood pressure, maximal oxygen consumption, respiratory and other equivalent assess the functionality of the subject.

A method of assessing the individual dynamics of changes in the functional capacity of the organism (Patent RU 2208387, AV 5/00; published 20.07.2003), based on the estimated time dynamics of body weight, chest circumference and abdominal circumference;comparison of the difference between these parameters and observing the changes in the functional capacity of the organism.

A method of assessing the functional capacity of the cardiorespiratory system, including the registration of tidal volume and simultaneously stroke volume of the heart and according to the formula calculate the reserve of the cardiorespiratory system (and.with. The USSR 1833714, AV 5/08 published 20.06.2003).

Known method of assessment of functional reserves of the human body (Patent RU №2463593 C1, G01N 33/48, G01N 33/497, published on 10.10.2012), the essence of which is to conduct hypoxic stress test using normobaric oxygen-depleted gas mixtures and subsequent fixation time reoxygenation with monitoring of physiological parameters. Then carry out the calculation of the coefficient of functional abnormalities in conventional. units and its value determine the level of functional reserves.

It is known that the question of assessing the reserve capacity of the immune system is not well developed, although the principle of mobilization of protective adaptive reactions of the organism is fundamental to maintaining normal levels its vital constants. The reserves of the immune system should be evaluated on the data of its potential activity on functional probe, and the degree of mobilization of functional reserve of T cells optimally characterized by the value of total and active T-lymphocytes:/�and. (I. B. Ushakov, Lapaev E. V., marianowski A. A. Immunological health of flight crews: concepts and assessment approaches // Military Medical journal, 1998, No. 12. P. 43-50). As a functional load for information about the data the potential activity of the immune system, these authors used a local hypoxic stress. The authors determined the reserve capacity of the cellular elements of the immune system as sufficient when the ratio We/and That We/T teophelinresistent lymphocytes is greater than 1, and decreasing this value of the index determined on the specific voltage of the mechanisms of resistance and increased mobilization of the immune system.

It is known that the immune system is one of the links, providing homeostasis, and plays a significant role in the adaptation to various perturbing influences, including significant physical stress (Surkin I. D., gotovtseva E. P. the Role of the immune system in the processes of adaptation in athletes // Theor. and practice. of physical culture. - 1991, №8. - Pp. 27-37). It is shown that the dynamics of indicators of immunity depends on the volume and intensity of the loads (Ivanova N. And., Talc, V. V. Influence of physical activity on the immune system // Theory and practice of physical culture. 1981, №1, p. 82-83), besides physical activity as a stress test causes options�national power of all adaptive systems (A. V. Burstow Standardized exercise test in the diagnosis of neurocirculatory dystonia. Clinical medicine, 2007, vol. 85, No. 5, Pp. 41-43).

Since physical activity is correlated with indicators of urgent adaptation of the immune system (A. E. sweet clover, author. Cand. dis. honey. of Sciences, Moscow, 2009, 24 p.), as the closest technical solution, we opted for this invention, which reflects the study of response of peripheral blood leukocytes, which is the seat of the immune system cells (Yarilin A. A. fundamentals of immunology. - M.: Medicine, 1999. - 608 p.), dosed physical load.

The closest technical solution according to the General methods of estimating the change in leukocyte peripheral blood as a result dosed physical load our chosen method of assessing the adequacy of physical activity of adolescents involved in sports (see patent RU NO. 2468753, AB 10/00 published: 10.12.2012). The method consists in the fact that determine the performance of peripheral blood lymphocyte index, an index of adaptation SPNR, index of immunoreactivity of R & d, and then determine the coefficients adaptation for each indicator in the system when the marker is set to 0.4 Kli is (-1,3), when the marker of 0.41-0.5 to equal (-1,2), when the marker is 0.51-0.6 and equal to (-1,1), when the marker 0,61-0,7 - equal (+1), when the marker 0,71-0,8 - equal (+1,1), when the marker 0,81-1,0 - equal (+1,2), �arcare more than 1.0 - equal (+1,3), in the system SPNR when you marker up to 0.3 Xpdr set equal to (-1,3), when the marker 0.31 and 0.5 to equal (-1,2), when the marker 0,51-0,7 - equal (-1,1), when the marker of 0.71 to 0.9 - equal (+1), in token of 0.91-1,1 - equal (+1,1), when the marker 1,11-1,3 - equal (+1,2); when the marker more than 1.3 - equal (+1,3), in the system of R & d in the marker to 3.0 Kiir set equal to (-1,3), when the marker 3,01-5,0 - equal (-1,2); if token 5,01-7,0 - equal (-1,1), when the marker 7,01-9,0 - equal (+1), when the marker 9,01-11,0 - equal (+1,1), when the marker 11,01-13,0 - equal (+1,2), when the marker more than 13,0 - equal (+1,3). A summary indicator of adaptive capacity is determined by the formula: detergents=Xpsr+Kli+Kiir, and determine the total measure of the adaptive capacity of the organism of young sportsmen to the load - SPAW and after exercise - SPAW, calculate the adequacy of physical activity (FSA) functional status of adolescents involved in sports, according to the formula:

AFS=(SPAW:SPAW)×100%, and values of AFS less than or equal to 92.9% of people believe the level of adequacy of physical load is low, when the AFS equal to 93%-99,9% - average, and for values of the AFS equal to 100% or more is high.

However, this method does not give the accuracy and reliability of evaluation of the functional reserves of the organism.

The objective of the claimed invention - improving the accuracy and reliability of evaluation of the functional reserves of the organism.

The invention consists in the fact, the method of determination of immuno-functional reserve of the organism, including definition of leukocyte peripheral blood before and after exercise test, determination of the coefficient of adaptation for each indicator, the summation of these coefficients and the finding of a summary indicator of adaptive capacity, characterized in that as a stress test use speed submaximal workload, conduct differential allocation of various types of leukocytes from the blood to the drug, determine the level of total T cells and active T cells (TA), calculate the index Then/TA, mononuclear cells were incubated with granulocytes, evaluate the index granulocytopenia lymphocytes (NEEDLES) ratio granulocytecolony (GRL pin associated with three granulocytes) and granulocytecolony lymphocytes (GOFS - pin associated with one of the granulocytes) in the product, determine leukocyte indexes: lymphocytic index (LI), the index of immunoreactivity (MIT), the adaptation index (SPNR), determine the coefficients adaptation immuno-functional condition (K) for each indicator, namely, when the value of the token IF set to 0.51 Kli is (-1,3), when the marker 0,52-0,57 - equal (-1,2), when the marker of 0.58 to 0.63 - equal (-1,1), when the marker 0,64-0,69 - equal (+1), PrimeCare 0,70-0,75 - equal (+1,1), when the marker 0,76-0,81 - equal (+1,2), when the marker more 0,82 - equal (+1,3); if the value of the token SPNR 0.39 install Xpdr equal to (-1,3), when the marker 0,40-0,50 - equal (-1,2), when the marker is 0.51 to 0.61 - equal (-1,1), when the marker 0,62-0,72 - equal (+1), in token of 0.73-0.83 to - equal (+1,1), when the marker is 0.84 to 0.94 - equal (+1,2), when the marker more than 0.95 - equal (+1,3); if the token value of R & d was set to 5.0 Kiir is (-1,3), when the marker 5,01-6,0 - equal (-1,2), when the marker 6,01-7,0 - equal (-1,1), when the marker 7,01-8,0 - equal (+1), when the marker 8,01-9,0 - equal (+1,1), when the marker 9,01-10,0 - equal (+1,2), when the marker more than a 10.1 - equal (+1,3), when the marker value ITL (T-lymphocytes) to 1.0 set Kitl equal to (-1,3), when the marker 1,01-1,30 - equal (-1,2), when the marker of 1.31 to 1.60 - equal (-1,1), when the marker 1,61-1,90 - equal (+1), when the marker 1,91-2,20 - equal (+1,1), when the marker 2,21-2,50 - equal (+1,2), when the marker more 2,51 - equal (+1,3); if the value of the marker NEEDLE set to 0.004 of Cigl equal to (-1,3)when the marker of 0,005 0,060 - equal (-1,2), when the marker 0,061-0,116 - equal (-1,1), when the marker 0,117-0,172 - equal (+1), when the marker 0,173-0,228 - equal (+1,1), when the marker 0,229-0,284 - equal (+1,2), when the marker more 0,285 - equal (+1,3), sum To all indicators, find the total immuno-functional state of the organism, SPIFS by the formula SPIFS=Xpsr+Kli+French+Kill+Kill to load - SPIFS and after exercise - SPIFS expect specific coef�icient immuno-functional reserve UKIP by the formula UKIF=(SPIFS+SPIFS)/5 and determine the level of immuno-functional reserve UKIP, at values UKIF above +1,0 consider the level of immuno-optimal functional reserve, at values UKIF from 0 to 1.0 is satisfactory, with values UKIF below 0 - unsatisfactory.

Said technical result is achieved in that in the method of determination of immuno-functional reserve of the organism to explore its overall characteristics, including induced dosed submaximal load adaptive capacity of the organism (SPNR, LEE), immunoreactivity (MIT, ITL) and interaction immunogenicity cells (lymphocytes and granulocytes) subject to the NEEDLES, which enables to get more reliable data and extends the capabilities of the known method. Lymphocytes and monocytes of the peripheral blood belong to the mononuclear cells, other white blood cells - granulocytes (Lymphocytes: Methods: TRANS. angl. / Edited by J. Claus. - M.: Mir, 1990. - 395 p.).

The method is performed as follows.

From peripheral blood by the conventional method in two-stage gradient of ficoll-verografin isolated fraction of mononuclear cells and granulocytes invaded by immunological and allergological methods in gigienicheskikh research. Fedoseyev V. N., Poryadin G. V., Kovalchuk L. V., Chiradeep A. N., Kogan V. Yu. M.: Promedic. - 1933. - 320 p.) before and after the metered speed physical �of agrusti on a Bicycle Ergometer with the achievement of submaximal heart rate.

Granulocytes in the amount of 4×106incubated on glass top in medium 199 for 15 min, released adhering to the glass non-adherent cells from by removing them together with a nutrient medium, added to a monolayer of granulocytes observed in the outer glass mononuclear cells (1×106incubated for 15 min, fixed with glutaric aldehyde, ethyl alcohol, washed with distilled water and stained with azure-eosin by Romanovsky-Giemsa. Under a light microscope to calculate the percentage of drug lymphocytes, monocytes, segmented and band neutrophils, basophils, eosinophils, and lymphocytes forming granulocytic sockets (GRL), and interactive contact with one granulocytes (GOFS). In parallel to determine the relative content of total and active T-lymphocytes (Novikov D. K., Novikov V. I. Evaluation of the immune status. Moscow, Vitebsk, 1996. - 282 p.), using the mononuclear cell fraction.

Based on these data, determine the coefficients adaptation immuno-functional condition (K) for each indicator, namely, in the system when the marker is set to 0.51 Kli is (-1,3), when the marker 0,52-0,57 - equal (-1,2), when the marker of 0.58 to 0.63 - equal (-1,1), when the marker 0,64-0,69 - equal (+1), when the marker 0,70-0,75 - equal (+1,1), when the marker 0,76-0,81 - equal (+1,2), PrimeCare more 0,82 - equal (+1,3); system SPNR when marker is set to 0.39 Xpdr equal to (-1,3), when the marker 0,40-0,50 - equal (-1,2), when the marker is 0.51 to 0.61 - equal (-1,1), when the marker 0,62-0,72 - equal (+1), in token of 0.73-0.83 to - equal (+1,1), when the marker is 0.84 to 0.94 - equal (+1,2), when the marker more than 0.95 - equal (+1,3); in the system of R & d in the marker set to 5.0 Kiir is (-1,3), when the marker 5,01-6,0 - equal (-1,2), when the marker 6,01-7,0 - equal (-1,1), when the marker 7,01-8,0 - equal (+1), when the marker 8,01-9,0 - equal (+1,1), when the marker 9,0-10,0 - equal (+1,2), when the marker more than a 10.1 - equal (+1,3), in the system it'll, when marker is set to 1.0 Kitl equal to (-1,3), when the marker 1,01-1,30 - equal (-1,2), when the marker of 1.31 to 1.60 - equal (-1,1), when the marker 1,61-1,90 - equal (+1), when the marker 1,91-2,20 - equal (+1,1), when the marker 2,21-2,50 - equal (+1,2), when the marker more 2,51 - equal (+1,3); in the system of NEEDLES when marker is set to 0.004 of Cigl equal to (-1,3), when the marker of 0,005 0,060 - equal (-1,2), when the marker 0,061-0,116 - equal (-1,1), when the marker 0,117-0,172 - equal (+1), when the marker 0,173-0,228 - equal (+1,1), when the marker 0,229-0,284 - equal (+1,2), when the marker more 0,285 - equal (+1,3), sum To all indicators, find the total immuno-functional state of the organism SPIFS by the formula SPIFS=Xpsr+Kli+French+Kill+Kill to load - SPIFS and after exercise - SPIVA, counting specific coefficient immuno-functional reserve UKIP by the formula UKIF=(SPIFS+SPIFS)/5 and ODA�make the level of immuno-functional reserve UKIP, at values UKIF above +1,0 consider the level of immuno-optimal functional reserve, at values UKIF from 0 to 1.0 is satisfactory, with values UKIF below 0 - unsatisfactory.

Summing up To all indicators, find the total immuno-functional state of the organism SPIFS by the formula: SPIFS=Xpsr+Kli+French+Kill+Kill, determine the total immuno-functional state of the organism to the load - SPIFS and after exercise - SPIFS expect specific coefficient immuno-functional reserve UKIP by the formula: UKIP=(SPIFS+SPIFS)/5 and determine the level of immuno-functional reserve: at values UKIF above +1,0 consider the level of immuno-optimal functional reserve, at values UKIF from 0 to 1.0 is satisfactory, with values UKIF below 0 - unsatisfactory.

Physical activity was performed using veloergometry. Stepwise increasing up to a submaximal workload was given depending on the patient's age, according to the Protocol, starting at 50 W and increasing every 2 min by 25 W, evaluating the condition of the examined ECG, indicate levels of systolic and diastolic blood pressure and heart rate (Instrumental methods of investigation of the cardiovascular system. Ed.T.With. Vinogradova. - M.: Medicine, 1986, 412 S.).

Based on the formulae, R & d, SPNR (1. Patent RU №2468753, AB 10/00 published: 10.12.2012), lymphocytic index (LI) was calculated by the formula:

LI=L (%) n/N (%) where L - lymphocytes, neutrophils,

index of immunoreactivity (MIT) - according to the formula:

MIT=L(%)+e(%)/M(%), where e - eosinophils, M - monocytes;

the adaptation index (SPNR) - according to the formula:

SPNR=L (%) (%), where C is segmented neutrophils.

Index granulocytopenia lymphocytes (NEEDLES) was calculated by the formula: NEEDLES=GRL/GOFS, where GRL - percentage granulocytecolony lymphocytes (lymphocytes, the contact associated with the three granulocytes), GOFS - the percentage granulocytecolony lymphocytes (lymphocytes, the contact associated with one of the granulocytes (GOFS)).

The essential difference of the proposed method is that to determine the total immuno-functional state of the organism SPIFS the results of the study of the relative abundances of different types of leukocytes and subpopulations (lymphocytes; lymphocytes, forming granulocytic sockets; lymphocytes, interactive contact with granulocytes; total T cells, active T-lymphocytes, granulocytes, neutrophils, eosinophils, basophils) to submaximal load - SPIFS and after exercise - SPIFS expect specific coefficient immuno-f�nctioning reserve UKIP by the formula: UKIP=(SPIFS+SPIFS)/5 and determine the level of immuno-functional reserve: at values UKIF above +1,0 consider the level of immuno-optimal functional reserve, at values UKIF from 0 to 1.0 is satisfactory, with values UKIF below 0 - unsatisfactory.

An example implementation of the method.

Example 1. Alexey V., age 24 years, at the time of the survey healthy. In the last 12 months, double ill ORVI. The results of the survey to load: lymphocytes - 36%, eosinophils - 2%, basophils - 1%, monocytes - 5%, segmented neutrophils - 50%, stab neutrophils - 6%, 53%, Cycle - 30%; GRL - 3%, RMK - 25%. Perform the calculation cytological leukocyte indices and their corresponding coefficients immuno-functional condition: IF=0,642 (Kli=+1); SPDR=0,72 (Cpnr=+1); R & d=7,6 (Kiir=+1); ITL=1,766 (Kill=+1); ILG=0,120 (Kill=+1). Determine the total indicator of immuno-functional state of the body to load: SPIFS=+5,0.

The results of the survey after exercise: lymphocytes - 38%, eosinophils - 3%, basophils - 1%, monocytes - 4%, segmented neutrophils 48%, stab neutrophils - 6%, 51%, Tact - 26%; GRL - 5%, RMK - 28%. Carry out the calculation of indices of nonspecific reactivity and their respective coefficients immuno-functional condition: IF = 0,703 (Kli=+1,1) + SPNR = 0,791 (Cspr=+1,1) + R & d 10,25 (Kiir=+1,3) + ITL 1,961 (Kitl +1,1) + ILG = 0,178 (Kill=+1,1). Determine the total indicator of immuno-functional state of the body after exercise: SPIFS=+5,7=1,14. Calculated specific coefficient immuno-functionality�form of further reserve UCIF: (5+5,7)/5=2,1. Characterize the level of immuno-functional reserve as the best.

The method for determining the immune functional reserve of the organism, including definition of leukocyte peripheral blood before and after exercise test, determination of the coefficient of adaptation for each indicator, the summation of these coefficients and the finding of a summary indicator of adaptive capacity, characterized in that as a stress test use speed submaximal workload, conduct differential allocation of various types of leukocytes from the blood to the drug, determine the level of total T cells and active T cells (TA), calculate the index Then/TA, mononuclear cells were incubated with granulocytes, evaluate the index granulocytopenia lymphocytes (NEEDLES) the ratio granulocytecolony (GRL pin associated with three granulocytes) and granulocytecolony lymphocytes (GOFS - pin associated with one of the granulocytes) in the product, determine leukocyte indexes: lymphocytic index (LI), the index of immunoreactivity (MIT), the adaptation index (SPNR), determine the coefficients adaptation immuno-functional condition (K) for each indicator, namely, when the marker value WHETHER: 0.51 establish the Kli is (-1,3), when the marker 0,52-0,57 - equal (-1,2), when the marker of 0.58 to 0.63 - equal (-1,1), when the marker 0,64-0,69 - equal (+1), when the marker 0,70-0,75 - equal (+1,1), when the marker 0,76-0,31 - equal (+1,2), when the marker more 0,82 - equal (+1,3); if the value of the token SPNR 0.39 install Xpdr equal to (-1,3), when the marker 0,40-0,50 - equal (-1,2), when the marker is 0.51 to 0.61 - equal (-1,1), when the marker 0,62-0,72 - equal (+1), in token of 0.73-0.83 to - equal (+1,1), when the marker is 0.84 to 0.94 - equal (+1,2), when the marker more than 0.95 - equal (+1,3); if the token value of R & d was set to 5.0 Kiir is (-1,3), when the marker 5,01-6,0 - equal (-1,2), when the marker 6,01-7,0 - equal (-1,1), when the marker 7,01-8,0 - equal (+1), when the marker 8,01-9,0 - equal (+1,1), when the marker 9,01-10,0 - equal (+1,2), when the marker more than a 10.1 - equal (+1,3), when the marker value ITL (T-lymphocytes) to 1.0 set Kitl equal to (-1,3), when the marker 1,01-1,30 - equal (-1,2), when the marker of 1.31 to 1.60 - equal (-1,1), when the marker 1,61-1,90 - equal (+1), when the marker 1,91-2,20 - equal (+1,1), when the marker 2,21-2,50 - equal (+1,2), when the marker more 2,51 - equal (+1,3); if the value of the marker NEEDLE set to 0.004 of Cigl equal to (-1,3), when the marker of 0,005 0,060 - equal (-1,2), when the marker 0,061-0,116 - equal (-1,1), when the marker 0,117-0,172 - equal (+1), when the marker 0,173-0,228 - equal (+1,1), when the marker 0,229-0,284 - equal (+1,2), when the marker more 0,285 - equal (+1,3), sum To all indicators, find the total immuno-functional state of the organism SPIFS by the formula SPIFS=Xpsr+Kli+French+Kill+Ki�l to load - SPIFS and after exercise - SPIFS expect specific coefficient immuno-functional reserve UKIP by the formula UKIF=(SPIFS+SPIFS)/5 and determine the level of immuno-functional reserve UKIP, at values UKIF above +1,0 consider the level of immuno-optimal functional reserve, at values UKIF from 0 to 1.0 is satisfactory, with values UKIF below 0 - unsatisfactory.



 

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3 dwg, 2 ex

FIELD: medicine.

SUBSTANCE: invention deals with method of predicting level of arterial pressure in women of Russian nationality, born in Central Black Earth region of Russia. Method includes separation of DNA from lymphocytes of peripheral venous blood and analysis of genetic polymorphisms. +46G/A ADRB2 and 4a/4b eNOS by method of polymerase chain reaction Level of systolic arterial pressure in women in late pregnancy is predicted by results of multiple regression equation of the following type: Y1=15,455+2,544x1+9,946x2+0,736x3+4,716x4+0,185x5, where x1 is genetic variant in locus - 4a/4b eNOS, namely 4b4b=1; 4a4b=2; 4a4a=3; x2 is presence of preeclampsia in relatives: yes=0, no=1; x3 is level of systolic arterial pressure before pregnancy, mm Hg; x4 is presence of cardiovascular system pathology: yes=0, no=1; x5 is woman's weight before pregnancy, kg Level of diastolic arterial pressure in women in late pregnancy is predicted, for which purpose multiple regression equation of the following type is used: Y2=14,200+7,768x1-2,877x2+7,500x3+0,414x4+3,668x5, where x1 is genetic variant in locus - 4a4b eNOS, namely 4b4b+4a4b=1, 4a4a=0; x2 is genetic variant in locus +46G/A ADRB2, namely GG+GA=1, AA=0; x3 is presence of preeclapsia in relatives:yes=0, no=1; x4 is systolic arterial pressure before pregnancy, mm Hg; x5 is presence of cardiovascular system pathology: yes=0, no=1.

EFFECT: invention makes it possible to realise early prediction of increase of arterial pressure level in women in late pregnancy, will make it possible to form of women at the stage of pregravidal preparation and at early terms of pregnancy groups of high risk of developing hypertension in late pregnancy, as well as realise required therapeutic-preventive measures aimed at prevention of development of said pregnancy complication in due time.

2 dwg, 2 tbl, 1 ex

FIELD: medicine.

SUBSTANCE: method is based on contacting a membrane test strip with an analysed fluid sample and initiating thereby a motion along the test strip membranes of reagents being parts of the sample or coating the membrane, and forming the immune complexes to be detected in the course of reactions in the membrane pores or on the surface thereof. A distinguishing feature of the presented method for antigen detection is that the test strip is coated additionally within the test sample contact area with a certain amount of specific antibodies, which react to the detected antigen expected to be found in the sample, when a fluid front moves and block a certain number of binding sites. The number of the coating free antibodies is specified so that the low content thereof in the analysed sample being of no diagnostic importance ensures blocking the binding sites completely that prevents the antigen from binding in the analysed area of the test strip and from developing a destructive staining in the analysed area.

EFFECT: presented approach enables reliable diagnosing based on the detection results of the antigens of gastrointestinal disorders, avoiding the achievement of positive test results for the low-antigen samples, which testifies to no development of the disease in an individual.

1 tbl, 2 ex

FIELD: medicine.

SUBSTANCE: invention relates to field of medicine, in particular hepatology and infectious diseases, and can be used for determination of stage of fibrous process in monitoring of patients with chronic hepatitis C. To realise method levels of blood serum cytokins are determined in patients with chronic hepatitis C with diagnosed by means of biopsy or other non-invasive method stage of fibrous process 2 times a year, with further calculation of cytokine profile integral index (CPII) on their basis, at initial stage F0 growth of CPII higher than -8 testifies to debut of fibrous changes in liver (transition to stage F1), at initial stage F1, drop of CPII below -10 testifies to transition to stage F2, at initial stage F2 growth of CPII higher than -3 testifies to transition of fibrosis to stage F3, at initial stage F3 drop of CPII below -3 testifies to development of cirrhosis.

EFFECT: determination of stage of fibrous process in monitoring of patients with chronic hepatitis C.

3 ex, 1 tbl, 5 dwg

FIELD: medicine.

SUBSTANCE: invention relates to medicine, namely to a method of predicting spontaneous onset of pregnancy in women with I and II stage of external genital endometriosis. The essence of the invention consists in the fact that before treatment in peripheral blood of women with infertility, associated with I and II stage of external genital endometriosis determined is a relative quantity of IL- IL-1β + monocytes, and if the value of the said index is 50.0% and higher in the monocytic gate the spontaneous onset of pregnancy within a year after carrying out the surgical treatment of endometriosis is predicted.

EFFECT: application of the claimed method makes it possible to predict with high accuracy the spontaneous onset of pregnancy in the women with infertility in case of I and II stage of external genital endometriosis within a year after therapeutic laparoscopy, which makes it possible to select optimal tactics of the patients' management and estimate the necessity of administering them methods of assisted reproductive technologies.

1 tbl, 3 ex

FIELD: medicine.

SUBSTANCE: peripheral blood serum interleukin-6 is measured by an immunoassay; if the measured level is more than 5 pg/ml, a biocontrol session of the heart rate variability is predicted to be effective and is expected to represent the total spectrum power gain of the heart rate variability combined with a drop in a regulatory system strain index in relation to references.

EFFECT: method enables the more accurate prediction of the length of the biocontrol course in order to intensify the vagal effect on the heart rate.

1 tbl

FIELD: medicine.

SUBSTANCE: invention relates to field of biotechnology and immunology. Described is antibody, which specifically binds denatured CD70. Claimed group of inventions can be used in medicine.

EFFECT: disclosed is method of diagnostics, prediction, prevention and treatment of malignant tumours of ovaries, pancreas and other malignant tumours with application of antibodies.

5 cl, 10 dwg, 2 tbl, 9 ex

FIELD: medicine.

SUBSTANCE: group of inventions relates to medicine, namely to immunology, and can be used for the ex-vivo determination of the cancer treatment efficiency. For this purpose, a level of activated T-lymphocytes (CD3+ CD69+) in an organism is measured after the introduction to a subject of one or more doses of an immunogenic composition. The level of the activated T-lymphocytes (CD3+ CD69+) higher than approximately 10.4% testifies to the fact that the subject is such that demonstrates successful clinical outcome for treatment; that is an increase of the survival rate.

EFFECT: application of the level of the activated T-lymphocytes (CD3+ CD69+) makes it possible to apply them as a biomarker for the monitoring, modification or correction of cancer treatment.

7 cl, 1 ex, 2 dwg

FIELD: medicine, ophthalmology.

SUBSTANCE: in lacrimal liquid one should detect the content of interleukin 8 (IL-8) and that of interleukin 1 beta (IL-1β) to calculate prognostic coefficient (PC) due to dividing the first value by the second one by the following formula: At PC value being below 10.0 one should predict favorable disease flow, and at PC value being above 10.0 - unfavorable flow.

EFFECT: higher accuracy of prediction.

2 ex

FIELD: medicine, medicinal microbiology.

SUBSTANCE: method involves growing microorganism culture to be studied in solid nutrient medium followed by preparing microbial suspension and its incubation in the presence of lactoferrin. Control sample is prepared in parallel series. Control and experimental samples are incubated, supernatant is removed from bacterial cells and lactoferrin concentration is determined in supernatant of experimental and control sample by immunoenzyme analysis. Then anti-lactoferrin activity is calculated by difference of concentrations of residual lactoferrin in experimental and control samples. This method provides enhancing the sensitivity and precision in carrying out the quantitative evaluation of anti-lactoferrin activity in broad spectrum of microorganisms that is urgent in diagnosis and prognosis of diseases with bacterial etiology. Invention can be used in determination of persistent indices of microorganisms for assay of their etiological significance in pathological processes.

EFFECT: improved assay method.

3 tbl, 3 ex

FIELD: medicine, biology.

SUBSTANCE: invention relates to nutrient medium used for accumulation of cells for the following cytological and/or immunocytochemical analysis carrying out. Invention relates to medium containing salts NaCl, KCl, anhydrous CaCl2, MgSO4 x 6 H2O, MgCl2 x 6 H2O, Na2HPO4 x 2 H2O, KHPO4, NaHCO3, and also glucose and Henx's solution, 10% albumin solution and polyglucin taken in the ratio 1:1:1. Invention provides enhancing the preservation of cells.

EFFECT: improved an valuable properties of nutrient medium.

3 ex

FIELD: medicine, cardiology.

SUBSTANCE: in peripheral blood one should detect the level of CD95(+) and CD16(+) neutrophilic granulocytes and at combination of increased level of CD95(+) neutrophilic granulocytes by 4 times and more and CD16(+) neutrophilic granulocytes by 0.6 times against the norm with ECG signs of myocardial infarction one should predict lethal result of large-focal myocardial infarction.

EFFECT: higher accuracy of prediction.

FIELD: medicine, parasitology.

SUBSTANCE: one should carry out immunoenzymatic assay to detect diagnostic optic density and that of labeled immune complex in a plot's hole with tested serum measured in conventional units at wave length being 492 nm. One should calculate coefficient of antibodies concentration measured in conventional units by the following formula: CAC = (Odtsh - Odd) x 100, where CAC - coefficient of antibodies concentration, Odtsh - optic density of the hole with tested serum, Odd - diagnostic value of optic density, 100 - coefficient of serumal dilution. By CAC value one should detect the titer of antibodies to Lamblia intestinalis antigens to interpret results of the trial. The method enables to study the dynamics of disease flow.

EFFECT: higher efficiency and accuracy of diagnostics.

1 ex, 1 tbl

FIELD: medicine.

SUBSTANCE: the present innovation deals with studying and treating diseases of inflammatory, autoimmune and degenerative genesis. One should perform sampling of heparinized blood followed by its sedimentation to obtain blood plasma with leukocytes and centrifuging to isolate the latter which are washed against erythrocytic and serumal admixtures, and, also, it deals with calculating the number of cells in samples out of leukocytic suspension after incubation (B) for 1.5 h at 37 C in holes of plastic microplotting board, out of leukocytic suspension one should additionally prepare two samples, one should be applied to calculate total number of leukocytes before incubation (A), the second sample undergoes incubation at the same mode at addition of autoserum to calculate the number of cells remained after incubation (C). One should state upon adhesive properties of leukocytes by the index of spontaneous adhesion (D), where D=(A-B)/B.100%, and effect for enhanced cellular adhesion under the impact of autoserum should be detected by the value of K=(B-C)/C.100% at K ≥ 30%, where B - C - the number of cells undergone additional adhesion after addition of autoserum. The present innovation widens functional possibilities of the suggested method due to obtaining additional values depicting adhesive properties of blood leukocytes.

EFFECT: higher accuracy of detection.

FIELD: medicine, immunology.

SUBSTANCE: one should carry out reaction of blast-transformation, detect proliferation of T-lymphocytes activated with antibodies to CD3 in the presence of interleukin-7 (ACT IL-7) and in the presence of interleukin-7 and dexametazone (ACT IL-7 D), calculate the index for dexametazone action as the ratio of ACT IL-7 to ACT IL-7 D, moreover, the value of dexametazone action index being above 1.2 indicates increased production of cytokins that suppress T-lymphocytes in neonatals. The method enables to detect functional defect of immune system that characterizes neonatal period.

EFFECT: higher efficiency of detection.

2 ex

FIELD: medicine.

SUBSTANCE: method involves measuring forced exhalation volume per 1 s (FEV1) in l, full right ventricle evacuation time (RVE) in ms and angiotensin II value (AII) in ng/l. Discriminant relationship is built as D=0.504·RVE+3.038·FEV1 - 2.0·AII. D being less than 83.88, pulmonary hypertension occurrence is predicted within 1 year. D being equal to or greater than 83.88, no pulmonary hypertension is predicted to occur.

EFFECT: enhanced accuracy of prediction.

FIELD: medicine, medicinal immunology.

SUBSTANCE: method involves determination of heterophilic antibodies in human serum blood by the Paul-Bunnel's method relatively the level of circulating immune complexes, complement-activating properties of heterophilic antibodies by incubation of standardized ram erythrocytes with 0.8% serum for 30 ± 5 min and the following measurement of the erythrocytes lysis degree. The measurement of the effector function coefficient of heterophilic antibodies is carried out by the complement system Keff.f.h.a.-c.s. by the formula: Keff.f.h.a.-c.s. = Y/Tg.a. wherein Y means a lysis degree, %; Tg.a. means a reverse titer of heterophilic antibodies to ram erythrocytes. The damage assay is carried out by comparison of the immune status with the relative level of circulating immune complexes in serum. Method provides detection of preclinic from of immunodeficiency and autoimmune diseases that opens the possibility for their prophylaxis at most early stages of development. Invention can be used for assay of damage in the immune status in human serum blood.

EFFECT: improved method for assay.

5 tbl, 1 ex

FIELD: medicine.

SUBSTANCE: method involves concurrently examining anti-inflammatory IL-4 level in blood serum and lacrimal fluid. The value being within the limits of 60-70 pg/l in blood serum and 5-15 pg/l in lacrimal fluid, disease prognosis is considered to be unfavorable. The IL-4 concentration being within the limits of 90-100 pg/l in blood serum and 20-30 pg/l in lacrimal fluid, disease prognosis is considered to be favorable.

EFFECT: high accuracy of diagnosis.

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