Compositions and methods for oral cavity care

FIELD: medicine.

SUBSTANCE: group of inventions relates to medicine and is intended for oral cavity care. Compositions contain non-dialysable material of cranberry extract, in which non-dialysable material of cranberry extract is present in quantity, effective for inhibiting co-aggregation of oral cavity bacteria and/or inhibiting bacterial growth.

EFFECT: group of inventions makes it possible to increase efficiency of oral cavity care due to inhibition of bacterial growth and co-aggregation of bacteria.

15 cl, 5 tbl, 4 ex

 

Background of the invention

The present invention generally relates to compositions for the oral cavity, used to ensure hygienic oral care, and more particularly to containing adelitasway material (NDM) cranberry extract compositions for oral care, with improved efficacy against dental plaque.

Adhesion of bacteria to each other and to the surfaces of the structures of the oral cavity represents one of the main factors leading to the formation of plaque and tooth decay and periodontal diseases. It would therefore be useful to have compounds against aggregation, which can interrupt microbial adhesion and aggregation.

Adelitasway material (NDM) cranberry extract is a material with a high molecular weight, obtained from cranberry juice, as described in the publication Ofek, I., Goldhar J. and Sharon N. Anti-Escherichia coli adhesion activity of cranberry and blueberry juices. Adv. Exp. Med. Biol. 1996; 408: 179-183, and in U.S. patents Nos. 6303125 and 6843993, and each patent is fully incorporated into this description by reference.

In U.S. patent No. 5683678, the description of which is fully incorporated into this description by reference, discloses the anthocyanins isolated from cranberry. Weiss, E., Lev-Dor, R., Kashamn, Y., Goldhar, J., Sharon, N. and Ofek, I. JADA, 129, 1719 (1998) describe the inhibition of coaggregation bulk bacteria subno�about plaque with NDM cranberry extract. Weiss et al. also describe the in vitro assays to test the ability of cranberry NDM to inhibit or eliminate coaggregation.

In U.S. patents №№ 5840322, 6303125 and 6843993, the descriptions of which are fully incorporated into the present application by reference, describes compositions for oral care, containing NDM cranberry extract, which addresses coaggregation in in vitro assays at a concentration of 1250 ág/ml. it Was found that the means for rinsing the oral cavity, containing NDM cranberry extract, reduces the total number of bacteria. However, in a clinical trial, which investigated the effect of the composition for oral care, containing NDM cranberry extract, was not observed changes in plaque and gingival indices, and the results showed no clinical advantage over standard formulations for rinsing the oral cavity (Weiss, E., Kozlovsky, A., Steiberg, D., Lev-Dor, R., Greenstein, R., Feldman, M., Sharon, N. and Ofek, I., FEMS Microbiology Letters 232, (2004), pp.89-92).

A brief summary of the invention

In this area there is a need to provide compositions for oral care, is able to inhibit bacterial coaggregation and reduce the buildup of plaque.

In a first aspect the present invention relates to compositions for oral care, containing netalisire�th material of cranberry extract and an orally acceptable excipient, where adelitasway material of cranberry extract is present in the amount effective for inhibiting bacterial coaggregation. Preferably, the composition for oral care did not contain an ingredient or component that deactivates adelitasway material of cranberry extract. In another aspect, the invention relates to a method of inhibiting bacterial coaggregation in the oral cavity comprising introducing in the oral cavity the composition for oral care, containing an orally acceptable excipient containing a number of mediasizename material of cranberry extract, effective for inhibiting bacterial coaggregation.

Detailed description

It should be understood that the detailed description and specific examples, while indicating embodiments of the invention, are intended for illustrative purposes only and are not intended to limit the scope of the invention.

The following definitions and non-limiting guidelines must be considered when interpreting the description of the present invention described in this application. The headings (such as "Introduction" and "Summary") and subheadings (such as "Compositions" and "Methods") used in the present description, are intended only for General structuring of the discussed issues�owls within the description of the invention, and are not intended to limit the description of the invention or any aspect of it. In particular, subject matter disclosed in the "Introduction" may include aspects of technology within the scope of the invention and may not add up to a statement of the prior art. The subject matter disclosed in the "Summary" does not constitute a comprehensive or complete description of the entire scope of the invention or any embodiment. The classification or description of material within a section of this specification as having a particular application (for example, as being a ingredient in the form of "active substances" or "carrier") is for convenience, and do not mean to imply that the material, when used in any given composition, must necessarily or solely function in accordance with its categorization as described in the present description.

In the present description, references are not an admission that those references are prior art or have any relevance to the patentability of the disclosed in the present description of the invention. Any discussion of the content of the links listed in the Introduction, is intended simply to provide a General summary of assertions made by authors of the references, and not an assumption about the accuracy of the contents of such links.

Description and specific Prim�ry, while indicating embodiments of the invention, are intended for illustrative purposes only and are not intended to limit the scope of the invention. In addition, the enumeration of multiple embodiments having these features is not intended to exclude other embodiments having additional features, or other embodiments incorporating different combinations of these. Specific examples are presented for illustrative purposes of how to make and use the compositions and methods of the present invention, and unless there is other clear indications, they are not intended to represent the fact that these embodiments of the present invention were or were not obtained or tested.

Used in this description, the words "preferred" and "preferably" refer to embodiments of the invention, which provide certain benefits in certain circumstances. However, other options for implementation may also be preferred for the same or other circumstances. In addition, the enumeration of one or more preferred embodiments does not imply that there can be used other variants of implementation, and is not intended to exclude other variants of osushestvlenie� from the scope of the invention. In addition, the compositions and methods may include, consist essentially of or consist of the elements described in the present application.

Used in this description, the words "include", "contain" and their variants are not intended to limit, so that the enumeration of the items in the list is not intended to the exclusion of other like items that may also be used in the materials, compositions, devices and methods of the present invention.

Throughout the present description and the claims specifying a particular digital value (e.g., temperature, % of the mass. composition, etc.) is used to denote this value plus or minus an additional quantity that must be understood by the person skilled in the art, depending on the variable and the degree of measurement error, usually associated with this value. For example, the average person skilled in the art should be understood that this includes temperature variance of up to 10%, subject to the instrument used to measure temperature.

The expression "coaggregation" refers to the aggregation/adhesion of two or more bacteria, including bacteria of different species, and inhibition of coaggregation or adhesion relates in General to preventing the initial adhesion or aggregation of the bacteria.

The composition of the invention inhibit coir�delegation of one or more bacteria, selected from Streptococcus oralis, Fusobacterium nucleatum, Actinomyces maeslundii, A. viscosus and S. mutan. Other bacteria that can couragious in the oral cavity, also included in the scope of the invention.

In the method of inhibiting bacterial coaggregation the composition of the invention can be an integral part of the remedies for mouth rinse, toothpaste, dental cream or gel or tooth powder and applied during the regular brush, or the compositions can be formulated and packaged as a separate processing means and be applied separately before, after and/or in the interval between cycles regular brush. The applied composition can be applied with a toothbrush, rinsing, chewing and other means known in this field.

Composition

In one embodiment of the present invention relates to compositions for oral care, containing adelitasway material of cranberry extract and an orally acceptable excipient, where adelitasway material of cranberry extract is present in the amount effective for inhibiting bacterial coaggregation. In another aspect, the NDM cranberry extract is present in the composition in amounts effective to inhibit and/or prevent bacterial coaggregation in the oral cavity. Prefer�till then, NDM cranberry extract is present in amounts appropriate for the prevention or treatment of a condition caused by a bacterial Koirala, such as a condition selected from dental plaque, dental caries, bad breath, periodontal disease and gingivitis. Mainly, adelitasway material of cranberry extract is present in the composition at a concentration of 0.08-1,33 mg/ml. In a preferred embodiment of adelitasway material of cranberry extract is preferably present in a concentration of about 0.3% of the mass.

The composition according to the present invention inhibits bacterial coaggregation in the oral cavity. Components of standard formulations of the compositions for oral care may prevent the effectiveness of NDM cranberry extract in the inhibition of bacterial coaggregation. Some of these components renders NDM cranberry extract, and as a result, although some of the documents disclosed the use of NDM cranberry extract as antibacterial efficacy of the composition when tested in reality provide no improved efficiency, compared to standard formulations, means for rinsing the oral cavity, which did not contain NDM cranberry extract. Applicants have found�, what are some of the ingredients deactivated NDM cranberry extract. Accordingly, preferred embodiments of the invention relate to compositions that do not include components, a deactivation NDM cranberry extract. The applicants of the present invention surprisingly found a composition including NDM cranberry extract, which effectively inhibits bacterial coaggregation and does not interfere with the activity of NDM cranberry extract.

The applicants have found that a composition comprising adelitasway material of cranberry extract has inhibitory effect on coaggregation bacteria the oral cavity. It was found that certain components of standard compositions for oral care reduce the ability mediasizename material of cranberry extract to inhibit bacterial coaggregation, making it ineffective in reducing bacterial growth. Without intending to be bound by any theory of mechanism of action, applicants have found that compositions containing surface-active substances that inhibit the ability mediasizename material of cranberry extract to inhibit bacterial coadhesion. In particular, surface-active substances, such as poloxamer, inhibit the activity of NDM cranberry extract. The other component of the�options, such as certain flavors and certain essential oils that are present in conventional amounts. These components can be used in the context of the present invention, but in amounts smaller than normally used in compositions for rinsing the oral cavity. The person skilled in the art, using the presented in this description of the instructions can easily determine what ingredients commonly used in compositions of the means for rinsing the oral cavity (and their respective concentrations, inhibit or interfere with the activity of NDM cranberry extract.

In one embodiment of the orally acceptable excipient is a combination of water, alcohol and one or more humidifiers. In a preferred embodiment of the alcohol is an ethanol. Compositions for oral care also preferably include one or more humectants selected from sorbitol and glycerin, and combinations thereof.

The composition according to the present invention may also contain one or more additional funds, usually selected from anti-plaque, a bleaching agent, antibacterial agents, cleaning agents, flavouring tools, sweetener, adhesion agents, surfactants, modulate�s foam abrasive agents, agents which modify pH, humectants, agents, providing a pleasant feeling in the mouth, dyes, of agent for dealing with dental stone (anticellulite), the source of fluoride ion, a means of stimulating the production of saliva, nutrients, and combinations thereof. The various components that can be added to the composition include, for example, a sweetener, such as saccharin or saccharin sodium, alcohols, such as ethanol, sources of fluoride ions, such as sodium fluoride, as well as glycerin, sorbitol, propylene glycol, polyethylene glycols, alkylpolyglycoside (APG), Polysorbate, PEG40, castor oil, menthol and the like.

Flavoring agents among those that can be used in the present invention include any material or mixture of materials used to enhance taste of the composition. You can use any orally acceptable natural or synthetic flavoring, such as flavoring oils, flavoring aldehydes, esters, alcohols, and similar materials and combinations thereof. Flavoring agents include vanillin, sage, marjoram, parsley oil, peppermint oil Koloskova, cinnamon oil, oil of Wintergreen (methyl salicylate), peppermint oil, clove oil, Bay oil, anise oil, eucalyptus oil, citrus oils, fruit oils and essences including t�, which derived from lemon, orange, lime, grapefruit, apricot, banana, grape, Apple, strawberry, cherry, pineapple, etc., perfumes, obtained from beans and nuts, such as coffee, cocoa, Cola, peanut, almond, etc., adsorbed and encapsulated flavorings and mixtures thereof. Among the flavors that are included in used in the present invention, includes ingredients that provide fragrance and/or other sensory effect in the mouth, including cooling or warming effects. Such ingredients include menthol, methyl acetate, mutilated, camphor, eucalyptus oil, eucalyptol, anethole, eugenol, Cassia, Oksanen, [alpha]-irison, propanalol, thymol, linalool, benzaldehyde, cinnamaldehyde, N-ethyl-p-methane-3-carboxamid, N,2,3-trimethyl-2-isopropylmalonic, 3-1-methoxypropane-1,2-diol, cinnamaldehyde glycerinate (CGA), the Metonic glycerinate (MGA) and mixtures thereof. One or more flavoring agents optionally present in a total quantity from about 0.01% to about 5%, optionally in various embodiments from about 0.05 to about 2%, from about 0.1% to about 2.5%, and from about 0.1 to about 0.5%.

Sweeteners among those that can be used in the present invention include dextrose, Polydextrose, sucrose, maltose, dextrin, dried invert sugar, mannose,�iloso, ribose, fructose, levulose, galactose, corn syrup, partially hydrolyzed starch, hydrolyzed hydrolyzed starch, sorbitol, mannitol, xylitol, maltitol, isomalt, aspartame, neotame, saccharin and its salts, energy, intense sweeteners-based dipeptides, cyclamate, dihydrochalcone and mixtures thereof.

Providing a pleasant feeling in the mouth agents include materials, imparting a desirable texture or other sensation during the application of the composition. These may include agglomerated particles of silicon dioxide, which are designed to fracture under stirring, such as materials series SORBOSIL® BFG (for example, BFG 10, 50 BFG, BFG 100, etc.), silica CBT60S, CBT70 or AC33/43, produced by PQ Corporation, Valley Forge, Pennsylvania.

Colorants among those that can be used in the present invention include pigments, dyes, varnishes and agents, Shine or reflectivity, such as glazing agents. In various embodiments, the coloring agents are utilized to ensure the tooth surface with white or light color coatings for action as the indicator plots on the surface of the teeth, which effectively contacted by the composition, and/or to modify appearance, in particular color and/or opacity of the composition for increased�increasing attractiveness for the consumer. You can use any orally acceptable coloring agent, including dyes and pigments, FD&C (which were approved in the US and approved for use in food, pharmaceutical and cosmetic industries), talc, mica, magnesium carbonate, calcium carbonate, magnesium silicate, a magnesium silicate-aluminum, silica, titanium dioxide, zinc oxide, red, yellow, brown and black iron oxides, ammonium ferrocyanide and ferric iron, manganese violet, ultramarine, titaniu mica, bismuth oxychloride, and mixtures thereof. One or more coloring agents, optionally present in a total quantity from about 0,001% to about 20%, e.g., from about 0.01% to about 10% or from about 0.1% to about 5%.

The compositions of the present invention may further comprise an optional abrasive material used, for example, as a polishing agent. You can use any orally acceptable abrasive material, but the type, the grinding, the particle size and amount of abrasive should be selected so that tooth enamel is not excessively exposed to wear and tear during normal use of the composition. Suitable optional abrasive materials include silicon dioxide, for example in the form of precipitated silica or in the form of a mixture with alum, insoluble phosphates, carbon� calcium and mixtures thereof. Among insoluble phosphates that are used as abrasive materials, there are orthophosphate, polymetaphosphate and pyrophosphates. Illustrative examples include dicalcium orthophosphate dehydrate, calcium pyrophosphate, tricalcium phosphate, polymetaphosphate calcium and insoluble polymetaphosphate sodium.

The compositions of the present invention optionally contain a means to combat dental stone (anticellulite). Means to combat dental stone are among those that can be used in the present invention include salts of any of these funds, for example, their alkali metal salts and ammonium salts: phosphates and polyphosphates (for example pyrophosphates), polyaminopropyl acid (AMPS), polyolefin sulfonates, polyolefin phosphates, diphosphonates such as azacycloheptane-2,2-diphosphonates (e.g., azacycloheptane-2,2-difosfonovoy acid), N-methyl azacyclopenta-2,3-difosfonovoy acid, ethane-1-hydroxy-1,1-difosfonovoy acid (EHDP) and ethane-1-amino-1,1-diphosphonate, phosphonocrotonate acid. Useful inorganic phosphate and polyphosphate include monobasic, dibasic and tribasic sodium phosphates, sodium tripolyphosphate, metropolitical, pyrophosphates, mono-, di-, tri - and tetranitro, trimetaphosphate sodium, sodium hexametaphosphate and mixtures thereof.

The compositions of the present invention neobyazatel� contain a source of fluoride ion, used, for example, as a means against caries. You can use any orally acceptable source of fluoride ions in the form of particles, including fluoride and monophosphate potassium, sodium and ammonium, fluoride divalent tin, India fluoride, amine fluorides, such as olaflur (N'-octadecyltrimethylammonium-N,N,N'-Tris(2-ethanol)dihydrofolic) and mixtures thereof. One or more sources of fluoride ions are not necessarily present in an amount that provides a clinically effective amount of soluble fluoride ion in the composition for oral care.

The compositions of the present invention optionally contain stimulating salivation tool used, for example, in the relief of dry mouth. Can be used any stimulating salivation agent, including without limitation food acids such as citric, lactic, malic, succinic, ascorbic, adipic, fumaric and tartaric acid, and mixtures thereof. One or more stimulating salivation funds are not necessarily present in stimulating salivation effective total amount.

The compositions of the present invention do not necessarily contain the nutrient. Suitable nutrients include vitamins, minerals, amino acids and mixtures thereof. Vitamins include vitamins C and D, thiamine, Riboflavin, PA�totient calcium Niacin, folic acid, nicotinamide, pyridoxine, cyanocobalamin, para-aminobutanol acid, bioflavonoids, and mixtures thereof. Nutritional supplements include amino acids (such as L-tryptophan, L-lysine, methionine, threonine, levocarnitine and L-carnitine), lipotropic substances (such as choline, Inositol, betaine, and linoleic acid), and mixtures thereof.

In various embodiments, the oral composition in accordance with the present invention is not intentionally swallowed, but rather retained in the oral cavity for a time sufficient to make the intended impact. In other embodiments, portable (such as a lozenge, mint chewing gum, granule, wafer, liquid formulated for oral application of a small portable sprayer, liquid formulated for oral application of a small portable generating drops of the bottle or soft, plastic tablet oral composition intentionally swallowed, but not necessarily, after retention in the oral cavity for a time sufficient to make the intended impact.

Compositions for oral care of the various embodiments is preferably presented in the form of the means for brushing your teeth. The term "dentifrice", as used in sun�mu description, means paste, gel or liquid preparative form. The dentifrice may be in any desirable form, such as a toothpaste (including pasta with deep stripes, surface strips, multilayer, pasta with surrounding paste gel); powder; granules; means for rinsing the oral cavity; spolaskivateli the oral cavity; lozenge; dental gel; a periodontal gel; a liquid suitable for painting a dental surface; a chewing gum; a dissolvable, partially soluble or insoluble film or strip; a wafer; a wet cloth or gauze; implant; foam; pellet; dental floss, liquid, formulated for oral application in a small portable sprayer (spray bottle), liquid formulated for oral application in a small portable generating drops bottle, soft plastic tablet ("gum"), or any combination of them. Used in the present description, the term "orally acceptable carrier" refers to a material or combination of materials that are safe for use in the compositions of the present invention, commensurate with a reasonable ratio of benefit/risk.

The expression "orally acceptable basis" or "orally acceptable carrier" as used in the context of the present invention, the STP�denotes any basis, used in the preparation of any of the funds described above to clean the teeth. Suitable orally acceptable bases include, for example, one or more of the following substances: solvent, alkali agent, a moisturizer, a thickener, a surfactant, an abrasive material, means against Tartar, coloring agent, sweetening agent, a dye containing a potassium salt, an antibacterial agent, desensitizing agents, means of reducing color-changing dental plaque, and mixtures thereof.

The present invention also relates to a portable dispensing the product containing the composition for oral care in the form described above for cleaning teeth, where portable dispensing a selected product from the wafer, mint chewing gum, granules, pastilles, a small portable nebulizer containing this mixture in liquid formulated for oral application as a spray, a small portable bottle containing this mixture in liquid formulated for oral application in the form of drops, and soft, squeezable tablets.

Preferably, the specific materials and compositions to be used in the present invention, are respectively pharmaceutically or cosmetically acceptable, clinically effective and/or clinically spectacular�I. Used in the present description, such a "pharmaceutically acceptable", "cosmetically acceptable", "clinically effective" and/or "clinically effective" component is one that is suitable for use in humans and/or animals, and presented in an appropriate amount (clinically effective) amount to provide the desired therapeutic, prophylactic, sensory, decorative or cosmetic favorable effect without undue adverse side effects (such as toxicity, irritation and allergic response) commensurate with a reasonable ratio of benefit/risk.

Adelitasway material of cranberry extract (NDM) is prepared from concentrate cranberry juice. Cranberry juice contains materials of high molecular mass (NDM) that inhibit bacterial adhesion to host cells, as well as coaggregation many bacteria in the mouth. NDM cranberry extract was obtained according to the method described by Weiss E; Lev-Dor, R.; Kashmamn, Y.; Goldhar, J.; Sharon, N.; Ofek, Itzhak, J, Am. Dent. Assoc. 129, 1719 (1998).

Methods of application

The composition according to the present invention may be administered or applied to a human or other animal. The composition may be suitable for administration or application to the oral cavity of a human or animal for inhibition tion�hardware bacterial coaggregation. Accordingly, the present invention relates to compositions, as defined above, for use as a medicament or cosmetic agent.

The present invention also relates to compositions for oral care, containing adelitasway material of cranberry extract and an orally acceptable excipient, where adelitasway material of cranberry extract is present in the amount effective for inhibiting bacterial coaggregation. The present invention also relates to a method of inhibiting bacterial coaggregation in the oral cavity comprising introducing in the oral cavity the composition for oral care, containing an orally acceptable excipient containing a number of mediasizename material of cranberry extract, effective for inhibiting bacterial madheshi.

Composition containing NDM cranberry extract and an orally acceptable excipient, are also able to significantly inhibit bacterial coaggregation. The composition, in particular, is used for inhibiting bacterial coaggregation in the oral cavity. A medicament containing a composition in accordance with the present invention, may be administered to the patient.

Any and every reference cited in the present description, fully �clucene into it by reference. Now various options for implementation will be described with reference to the following non-limiting examples.

Certain embodiments of the invention

Example 1

Composition for mouth rinse containing cranberry NDM extract

NDM cranberry extract was obtained according to the method described by Weiss et al. J. Am. Dent. Assoc. 129(12), 1719 (1998). NDM cranberry extract were obtained by dialysis of cranberry juice using a dialysis bag with a cutoff of high molecular weight. The substance remaining in the pouch, which is not dialisys out, is adelitasway material (NDM). NDM cranberry extract is comprised in a composition for rinsing the oral cavity (shown in table 1).

Table 1
Composition for rinsing the oral cavity, containing NDM cranberry extract as the active ingredient
Component% of the mass.
96% ethanol (95% ethanol)6,00
NDM cranberry extract0,30
Deeply purified water73,58
Saccharin sodiumSodium fluoride according to the USP0,05
Sodium benzoate0,05
Sorbitol 70% solution (NB, NC)10,00
Glycerin 99% organic kosher10,00
TOTAL100,00

The in vitro analysis showed that the composition for rinsing the oral cavity with NDM cranberry extract is effective against coaggregation bacterial pairs of S. sangius and F. nucleatum, with 8-fold dilution.

The in vitro analysis also showed that the composition for rinsing the oral cavity exerted inhibitory effect on the growth of A. viscosus when diluted 25 times (table 4 and table 5).

Example 2

Composition for rinsing of a mouth which defeats the NDM cranberry extract

An example of a compound that deactivates NDM cranberry extract.

Table 2
An example of the composition of the NDM cranberry extract, which not inhibits bacterial coaggregation or height
Component% of the mass.
96% ethanol (95% ethanol)NDM cranberry extract0,35
Deeply purified water71,53
Saccharin sodium0,02
Sodium fluoride according to the USP0,05
Sodium benzoate0,05
Poloxamer 338 according to the National Pharmacopoeia1,00
Poloxamer 497 according to the National Pharmacopoeia1,00
Sorbitol 70% solution (NB, NC)10,00
Glycerin 99% organic kosher10,00
TOTAL100,00

The in vitro analysis showed that the composition for rinsing the oral cavity, shown in table 2, is not effectively inhibited bacterial coaggregation. Accordingly, the presence poloxamer inhibited or interfered with the activity of NDM cranberry extract. Specialists in this field can easily test other components and their respective quantities, using the above Protocol, to establish each�x usually of the components used in the compositions for rinsing the oral cavity, which inhibit or otherwise interfere with the activity of NDM cranberry extract.

A specific experimental method of analysis of anticoagulation described in the publication Weiss EI, Lev-Dor R, Kashamn Y, Goldhar J, Sharon N, Ofek I, "Inhibiting interspecies co-aggregation of plaque bacteria with a cranberry juice constituent," J. Am. Dent. Assoc. 1988 Dec; 129(12): 1719-23.

Example 3

Analysis of anticoagulation

Composition for rinsing the oral cavity, containing 0.3% NDM cranberry extract, and has the effect against coaggregation, and the effect of inhibition of bacterial growth. The results of anticoagulation shown in table 3, indicate that NDM cranberry extract effectively inhibits coaggregation bacterial pairs.

A specific experimental method of analysis of anticoagulation described in the publication Weiss EI, Lev-Dor R, Kashamn Y, Goldhar J, Sharon N, Ofek I, "Inhibiting interspecies co-aggregation of plaque bacteria with a cranberry juice constituent," J. Am. Dent. Assoc. 1988 Dec; 129(12): 1719-23.

The results shown in table 3, indicate that NDM cranberry extract in the pure solution is an effective inhibitor of bacterial madheshi.

Table 3
The test results anti coaggregation NDM cranberry extract in pure solution
The final concentration (mg/ml)NDM I NDM INDMIINDMII
Act/SmSo/FnAct/SmSo/Fn
1,330000
0,660000
0,330000
0,161010
0,083434
Negative control3434
Note:
0 = no coaggregation (full inhibition);
4 = full coaggregation (no inhibition);
So/Fn: Streptococcus oralis/Fusobacterium nucleatum;
Act/Actinomyces naeslundii/S. muta.

Example 4

Analysis of inhibition of growth

The bacterium A. viscosus bred from a single colony growing on the Cup with blood agar. Them aseptically transferred into the centrifuge tube containing 30 ml of sterile TSB medium. Then the centrifuge tube was placed in an incubator at 37.5°C for growing during the night. The next day, the bacterial solution were stained with gram to determine the purity and then diluted to an optical density of 0.23 at 610 nm on a UV spectrometer. The volume of 9.6 ml of inoculum was added to the falcon tube with 0.4 ml of the test solution for rinsing the oral cavity to obtain the final dilution of the solution to rinse 1:25. Then the tubes were incubated in a shaken water bath at 37,5°C. After certain time intervals, 1 ml was removed from the tubes and placed in a cuvette to obtain a UV spectrum.

This in vitro analysis showed that the composition for rinsing the oral cavity with NDM cranberry extract had inhibitory effect on the growth of A. viscosus at 25-fold dilution (table 4 and table 5).

Table 4
The test data of inhibition of bacterial growth of A. viscosus
Sample0 hours2 hours 4 hours22 hours24 hours
Water0,23410,36030,57561,58481,7604
The solution for rinsing the oral cavity with placebo0,23410,33760,52341,45581,5740
The solution for rinsing the oral cavity with 0.3% NDM cranberry extract0,23410,17030,19180,78791,0405

Table 5
Interest rate reduction after 24 hours based on the data of table 4
The solution for rinsing the oral cavity with placebo10,6%
The solution for rinsing the oral cavity with 0.3% NDM cranberry extract40,9%

The invention has been described above with reference to illustrative examples, but it should be understood that the invention is not limited to the OPI�data available with the options of implementation. Changes and modifications will become apparent to the person skilled in the art after reading the description, also included in the scope of the invention as defined in the attached claims.

1. Composition for oral care, containing adelitasway material of cranberry extract and an orally acceptable excipient, where adelitasway material of cranberry extract is present in a concentration of from 0.66 to 1.33 mg/ml, where the composition does not contain surfactants, which deactivates adelitasway material of cranberry extract, and where the composition is a means for rinsing the oral cavity.

2. Composition for oral care according to claim 1, wherein the orally acceptable excipient selected from one or more of the group consisting of water, alcohol, humidifier, and combinations thereof.

3. Composition for oral care according to claim 2, where the alcohol is an ethanol.

4. Composition for oral care according to claim 3, wherein the alcohol is present in a concentration of about 6% wt./masses.

5. Composition for oral care according to claim 2, where the humectant is selected from the group consisting of one or more of sorbitol, glycerol and mixtures thereof.

6. Composition for oral care according to claim 5, where the humectant is present at a concentration of about 10% wt./masses.

<> 7. Composition for oral care according to claim 1, where the composition further comprises one or more means selected from the group consisting of anti-plaque, a bleaching agent, preservative, sweetener, cleansing products, flavoring funds and their mixtures.

8. Method for inhibiting bacterial coaggregation in the oral cavity comprising introducing in the oral cavity the composition for oral care, containing an orally acceptable excipient containing adelitasway material of cranberry extract in a concentration of from 0.66 to 1.33 mg/ml, where the composition does not contain surfactants, which deactivates adelitasway material of cranberry extract, and where the composition is a means for rinsing the oral cavity.

9. A method according to claim 8, wherein the orally acceptable excipient selected from one or more of the group consisting of water, alcohol, humidifier, and combinations thereof.

10. A method according to claim 9, where the alcohol is an ethanol.

11. A method according to claim 10, wherein the alcohol is present in a concentration of about 6% wt./masses.

12. A method according to claim 9, where the humectant is selected from the group consisting of one or more of sorbitol, glycerol and mixtures thereof.

13. A method according to claim 12, wherein the humectant is present at a concentration of about 10% wt./masses.

14. A method according to claim 8, wherein to�position contains one or more additional funds, selected from the group consisting of anti-plaque, a bleaching agent, preservative, sweetener, cleaning agents and flavoring funds.

15. Composition for oral care, containing an orally acceptable excipient and a means for inhibiting bacterial coaggregation, where the specified means consists essentially of mediasizename material of cranberry extract in a concentration of from 0.66 to 1.33 mg/ml, where the composition does not contain surfactants, which deactivates adelitasway material of cranberry extract, and where the composition is a means for rinsing the oral cavity.



 

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3 ex

FIELD: medicine.

SUBSTANCE: opening of a carious cavity, ablation of overhanging enamel edges on the entire circumference, necrectomy and drug treatment with a 0.06% chlorhexidine solution are performed. The preparation "Lemifaren" is applied on walls and bottom of the carious cavity in a uniform 1 mm thick layer, which is left for 1 day under temporary filling. After the removal of the preparation irradiation in a pulse mode with the power of 5 W and frequency of 2000-3000 Hz is carried out for 5 min with the application of the laser apparatus ALST-01. After that, the cavity is restored with an account of functional and aesthetic parameters of the particular tooth.

EFFECT: effective treatment of deep caries prevents caries recurrences and pulp inflammation.

1 ex

FIELD: medicine.

SUBSTANCE: group of inventions relates to medicine and is intended for oral cavity care. Compositions contain: a) guanidine-based active substance, b) film-forming polymer, c) hydrophobic modifier of viscosity in quantity, sufficient for provision of composition for oral cavity care with time particle sedimentation more than 20 minutes, and d) oil carrier. Guanidine-based active substance preferably represents L-arginine. Film-forming polymer preferably represented GANTREZ. Hydrophobic modifier of viscosity preferably represents thickened mineral oil. Oil carrier preferably represents natural oil. Composition is enclosed in gelatine capsule. Method of teeth cleaning includes application on teeth of composition for oral cavity care in such a way that composition for oral cavity care cleans teeth. Device for oral cavity care, which contains: handle, fastened on handle head, where head has external surface and a row of teeth-cleaning elements, projecting outwards on external surface, and gelatine capsule, which contains composition for oral cavity care, located on head.

EFFECT: group of inventions makes it possible to increase teeth cleaning efficiency.

14 cl, 2 ex, 1 tbl

FIELD: medicine.

SUBSTANCE: implementing the presented method involves mixing gel Hyaludent 1 ml on a slide with Betaleukin 0.0005 ml dissolved in water for injections 1 ml; the produced content is applied with a single application syringe on a desalivated treated area of the involved periodontal tissues and left until completely absorbed for 1-3 minutes; the involved area is exposed to laser light generated by the laser semiconductor dental therapeutic apparatus Optodan with a periodontal tip in the mode II, with the exposure length for 3-5 minutes; the therapeutic course is 8 days daily.

EFFECT: using the method enables achieving the fast regeneration length, ensuring the intact periodontal state in the most patients.

4 tbl, 3 ex

FIELD: medicine.

SUBSTANCE: invention represents an agent for treating a pulp inflammation differing by the fact that it contains Bezornil ointment and Dycal ivory paste in ratio 1:1 blended until smooth.

EFFECT: invention provides relieving oedema and pain on the day of doctor's appointment, accelerating the pulp tissue regeneration process, creating the reparative dentin within 30 days, and ensures the higher clinical effectiveness in the pulp inflammations.

2 ex, 1 tbl

FIELD: medicine.

SUBSTANCE: a phytopreparation containing bee wax 1.9 g and a herbal extract 0.1 g with the above herbal extract containing common oak bark, common St. John's wort herb and creeping thyme (Thymus) herb in equal proportions is used.The preparation is used as a chewing substrate (a chewing gum) for two weeks 3 times a day 15 minutes after meals.

EFFECT: using the invention improves clinical manifestations of the disease; oral inflammations are supposed to relieve or reduce; the length of erosion and ulcer epithelisation is reduced; the oral fluid structural properties are normalised by increasing phosphorus and magnesium, reducing protein, calcium and TBA.

1 tbl, 1 ex

FIELD: medicine.

SUBSTANCE: vitamins A and E, novocaine blocades of parotid salivary glands, intake of potassium iodide solution, intake of galantamine, additionally administered is "Vezugen" in a dose of 2 tablets (capsules) 2 times per day for 30 days are administered.

EFFECT: invention makes it possible to stimulate the formation of own saliva in patients in case of drug sialoadenopathy, conditioned by chemotherapy of malignant tumours.

2 ex

FIELD: medicine.

SUBSTANCE: 20-25 minutes after surgical intervention, a mouth bath of an implantation area with the Tonsinal phytopreparation diluted in an amount of 2.5 g in 100 ml of boiled water of room temperature is executed. 20-25 minutes later the Plastin CM-1 phytopreparation is applied on the alveolar process mucosa within the implantation area from the vestibular side for 1.5-2 hours. That is preceded by the desalivation of the oral cavity and mouth rinsing carefully with the Tonsinal solution trying to avoid any dynamic movements. The above procedures are performed 3-4 times a day for at least 10 days.

EFFECT: more effective dental implantation by improving microcirculation, fast normalising of oral microbiocoenosis, wound surface healing by immediate union, reliable implant attachment to gums and providing the implant stability.

3 ex

FIELD: medicine.

SUBSTANCE: group of inventions relates to medicine, namely to dentistry, and can be used for the treatment of conditions, related with the increased expression of MMP-13, selected from the group, consisting of loss of one or more teeth fixation, teeth loss, teeth mobility, formation of recesses, bone tissue loss and a combination of two and more of the said conditions. For this purpose the application of 4-acetamidophenyl 2-isopropyl-5-methylcyclohexylcarbonate in the production of a medication for the treatment of the said conditions is claimed. A composition for teeth care, containing an orally acceptable carrier and a therapeutically effective quantity of a MMP-13 inhibitor in the form of 4-acetamidophenyl 2-isopropyl-5-methylcyclohexylcarbonate is also claimed.

EFFECT: group of inventions provides the effective treatment of the said conditions die to effective MMP-13 suppression.

3 cl, 5 tbl, 5 ex

FIELD: medicine.

SUBSTANCE: method involves professional oral hygiene is carried out consisting in ultrasonic removal of supra- and subgingival dental deposits and polishing of supragingival teeth. Bite splinting and recovery of dentition integrity may be required. After dissecting a mucoperiosteal flap according to the known technique, an incision area is sanitated by means of a photodynamic therapy (PDT). The PDT is conducted with the use of a diode laser at wave length 660±5 nm and emitting power 0.5-1.0 Wt. The photosensitiser "Photoditasin" in the form of 0.5% gel is introduced by means of a cannula into dental gaps, under the dissected segments of the flap and onto the mucosal tissue for 5 minutes. The photosensitiser is washed out, and the gingival pockets are repeatedly exposed to laser light for 2-3 min in the same environment. Sterile osteoplastic material is introduced into bone defects, and the flap is sutured together.

EFFECT: effective cleansing of the surgical area, eliminating the periodontal inflammation, stimulating tissue osteogenesis and regeneration, stabilising the processes of bone tissue absorption of alveolar interdental septa and preserving the tissues.

2 cl, 1 ex

FIELD: veterinary medicine.

SUBSTANCE: method comprises subcutaneous administration of antibiotic preparation enroxyl 5% at a dose of 0.1 ml/kg daily one time a day for 7 days and intramuscular administration of homeopathic preparation ovarinin at a dose of 1 ml/kg one time for 4 days, 4-fold.

EFFECT: use of the invention enables to increase the efficiency of treatment, to reduce treatment time and to restore reproductive function of dogs.

2 tbl, 1 ex

Black adler syrup // 2557929

FIELD: medicine.

SUBSTANCE: black adler syrup having laxative action, characterised by the fact that it is produced by preparing black adler tea by boiling the raw material with purified water; the tea is further mixed with refined sugar; the mixture is heated until the sugar is dissolved completely and brought to boil twice with skimming as often as it may be required; the prepared syrup is filtered through gauze in the certain environment.

EFFECT: syrup is high in anthracene derivatives and possesses the pronounced laxative action.

2 dwg, 1 ex

FIELD: medicine.

SUBSTANCE: invention refers to medicine and cosmetology, namely to deep cosmetic products, and can be used for normalising physiological functions of skin and eliminating endogenous or exogenous defects. The invention represents a cosmetic product for fast skin repair containing superoxide dismutase, dexpanthenol, succinic acid, trolox and L-arginine. The product is applied on the skin for 20 minutes with using a material holding the agent and feeding it to the skin over the period of exposure.

EFFECT: cosmetic product enables extending the range of products for improving the skin state, reducing the risk of lower controllability of the effect, and a probability of allergic and inflammatory reactions.

3 cl, 10 ex

FIELD: medicine.

SUBSTANCE: using a polyphenolic complex produced by extracting milled ash berry in 40% ethanol, condensing the alcohol-water extract, adding 95% ethanol, centrifuging the residue, filtering and condensing the supernatant in the certain environment, as an agent possessing anti-inflammatory action.

EFFECT: polyphenolic complex possesses pronounced anti-inflammatory action.

1 dwg, 9 tbl, 7 ex

FIELD: medicine.

SUBSTANCE: group of inventions refers to veterinary science and is applicable for treating bovine mastitis. What is declared is a nosode for producing a preparation for treating bovine mastitis. That involves taking mastitis milk 1 ml with clinical signs of purulent-catarrhal mastitis, filling it 70° alcohol 9 ml to produce a homogenous solution; the produced solution is diluted to "Д6" with 70° alcohol. What is also declared is a preparation for treating bovine mastitis containing an aqueous-alcoholic solution of components. The components are Belladonna, Sulphur, Apis mellifica, Conium, Phytolacca, Silicea and above nosode in the following proportions, wt %: Belladonna - 5, Sulphur - 20, Apis mellifica - 20, Conium - 10, Phytolacca - 10, Silicea - 5, nosode - 15, aqueous-alcoholic solution up to 100%. What is also declared is a method of treating bovine mastitis involving the intracisternal administration of the preparation. The preparation is administered in a dose of 5-10 ml per 1 animal once a day.

EFFECT: declared group of inventions is highly effective in treating bovine mastitis.

3 cl, 5 tbl, 1 ex

FIELD: medicine, pharmaceutics.

SUBSTANCE: invention relates to pharmaceutical composition, containing compound of formula or for prevention or treatment of diseases, associated with oxidative stress, selected from group, consisting of MELAS (mitochondrial myopathy, encephalopathy, lactic acidosis and stroke episodes), MERRF syndrome (myoclonic epilepsy with ragged red fibres) or Kearns-Sayre syndrome, arrhythmia, cardioplegia or myocardium infarction. in formula (1) na stands for 1 or 2, Aa represents 5-membered heteroaryl or heterocycle, each of which has 2 heteroatoms, selected from N, O and S, Rla represents R5a-Xa-Ba-X′a-, Ba represents direct bond, Xa and X′a independently on each other represent direct bond or -OC(O)-, R5a represents hydrogen or 6-9-membered monocyclic or condensed cyclic heterocycle or heteroaryl, each of which has from 1 to 3 heteroatoms, selected from N, O and S, and is optionally substituted with oxo or C1-C6-alkyl, R2a represents -(CR8aR9a)pa-Ya-R7a, pa stands for number from 0 or 1, Ya represents direct bond or -O-, R7a represents hydrogen or phenyl, R3a, R8a, R9a, R10a represent hydrogen, R4a represents -(CH2)pa-Da-R10a-, Da represents C5-cycloalkyl or 6-membered heterocycle, which has 1 heteroatom, selected from N, S and O. Radical values for formula (2) are give in invention formula.

EFFECT: obtaining compositions for prevention or treatment of diseases, associated with oxidative stress.

19 dwg, 5 tbl, 3 ex

FIELD: medicine.

SUBSTANCE: opening of a carious cavity, ablation of overhanging enamel edges on the entire circumference, necrectomy and drug treatment with a 0.06% chlorhexidine solution are performed. The preparation "Lemifaren" is applied on walls and bottom of the carious cavity in a uniform 1 mm thick layer, which is left for 1 day under temporary filling. After the removal of the preparation irradiation in a pulse mode with the power of 5 W and frequency of 2000-3000 Hz is carried out for 5 min with the application of the laser apparatus ALST-01. After that, the cavity is restored with an account of functional and aesthetic parameters of the particular tooth.

EFFECT: effective treatment of deep caries prevents caries recurrences and pulp inflammation.

1 ex

FIELD: medicine.

SUBSTANCE: method involves administering an anthelminthic agent in the period of stationary process stage; the anthelminthic treatment is preceded by administering chophytol 200 mg 3 times a day before meals for 14 days; the anthelminthic treatment involves administering Osinol food supplement 2 tablets 3 times a day during meals for 14 days; thereafter, chophytol is taken in a dose of 200 mg 3 times a day before meals for 14 days; 7 days later, the repeated course of the anthelminthic treatment with Osinol food supplement 2 tablets 3 times a day during meals is prescribed to be administered for 14 days; the entire therapeutic course involves washing the face with the Setaphyldermocontrol foaming facial cleanser 2 times a day followed by applying an SPF30 product on the face once after the morning wash.

EFFECT: reducing the number of complications and the high efficacy of the anthelminthic treatment.

1 ex

FIELD: chemistry.

SUBSTANCE: invention relates to cosmetic industry and provides a composition for depositing on keratin surfaces, which contains composition particles in the form of fused agglomerates having a part containing at least one dye and a part containing at least one transparent and translucent thermoplastic material, where the composition particles contain, based on the weight of all of the powdered composition material, about 10 to 90 parts of the dye part and about 90 to 10 parts of the transparent and translucent thermoplastic material part, and a method of producing the powdered composition material.

EFFECT: invention enables to obtain cosmetic compositions which will have an improved outer appearance on the skin and fewer SKU.

23 cl, 10 tbl, 11 ex

FIELD: medicine.

SUBSTANCE: group of inventions relates to medicine and is intended for oral cavity care. Compositions contain: a) guanidine-based active substance, b) film-forming polymer, c) hydrophobic modifier of viscosity in quantity, sufficient for provision of composition for oral cavity care with time particle sedimentation more than 20 minutes, and d) oil carrier. Guanidine-based active substance preferably represents L-arginine. Film-forming polymer preferably represented GANTREZ. Hydrophobic modifier of viscosity preferably represents thickened mineral oil. Oil carrier preferably represents natural oil. Composition is enclosed in gelatine capsule. Method of teeth cleaning includes application on teeth of composition for oral cavity care in such a way that composition for oral cavity care cleans teeth. Device for oral cavity care, which contains: handle, fastened on handle head, where head has external surface and a row of teeth-cleaning elements, projecting outwards on external surface, and gelatine capsule, which contains composition for oral cavity care, located on head.

EFFECT: group of inventions makes it possible to increase teeth cleaning efficiency.

14 cl, 2 ex, 1 tbl

FIELD: medicine, oncology, amino acids.

SUBSTANCE: invention relates, in particular, to the development of an antitumor preparation based on natural substances. Invention relates to an amino acid preparation comprising at least one modified essential amino acid obtained by treatment of amino acid by ultraviolet radiation (UV) at wavelength 250-350 nm for 12-80 h at temperature 15-30oC or with ozone at temperature 15-25oC. The modified amino acid has no toxicity for health cells. Also, invention relates to a method for preparing such preparation. Invention provides the development of an antitumor preparation based on modified amino acids and expanded assortment of antitumor preparations being without cytotoxicity for normal cells.

EFFECT: valuable medicinal antitumor properties of preparation.

8 cl, 4 tbl, 2 dwg, 4 ex

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