Method of intravital collection of fertilised eggs (in vitro) from causative agent of parasitic zoonosis trichuris (=trichocephalus) vulpis

FIELD: veterinary medicine.

SUBSTANCE: method comprises selection of only living, mature females of Trichuris vulpis from colon, blind gut of wild and/or domestic carnivorous animals infected spontaneously with whipworms in the study with helminthological methods when autopsy, into separate tubes with officinal isotonic solution (0.9%) of sodium chloride (solutio Natrii chlorati isotonica) and the exposure of the tubes with the females of Trichuris vulpis at t = 37.5-39°C for 5 hours under conditions of a thermostat.

EFFECT: method enables to select a large amount of fertilised eggs of T vulpis, not contaminated with particles of undigested feed, the particles of destroyed tissue of genitals of female of T vulpis and secondary bacterial microflora.

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The invention relates to the field of veterinary medicine and, more particularly, to Parasitology (= helminthology) to "the Way of lifetime of collecting fertilized eggs (in vitro) from the pathogen parasitic zoonosis Trichuris (= Trichocephalus) vulpis", and is intended for use when a parasitological laboratory studies, in vivo (in an artificial environment and at a temperature characteristic of carnivorous) fees fertilized eggs from living, sexually Mature females Trichuris (= Trichocephalus) vulpis, the resulting opening by the method of K. I. Skryabin [9], intestine and the cecum spontaneously infected with trichuriasis wild and/or domesticated carnivorous.

It is known that Trichuris (= Trihocephalus) vulpis (Froelich, 1789) is the causative agent of trichuriasis Pets (dogs), wild (wolves, ferrets, foxes, brown bears in the conditions circuses) and predatory humans and the causative agent of parasitic zoonosis continues to be a cause of severe disease not only predatory, but man, sometimes resulting in the need to apply surgical methods (appendectomy) for its treatment [Pasechnik V. E. 3, 4, 5, 6, 7; K. I. Skryabin, Petrov A. M. - 10; Kenney, M.; Eveland, L. K. - 3; Kenney, M; Yermakov, V. 3].

Worms: Trichuris (= Trichocephalus) vulpis remains in the spotlight and who at this stage of time, as it can affect people, especially children, along with another parasite Trichuris trichira, L., 1771 - the causative agent of trichuriasis humans, monkeys and in larval rearing wild and domestic pigs [Pasechnik V. E. - 3, 5; T. Sakano, et al. - 11; S. Singh, et al. - 12].

Morbidity of population with trichuriasis in 2004 were recorded in all subjects of the Russian Federation [Romanenko N. And., Malysheva N. With. - 8, p. 30]. In the period 1999-2002 all incidence trichuriasis in Moscow, Nenets, Khanty-Mansi Autonomous districts, the Republic of Sakha (Yakutia), Kamchatka, Tambov regions were formed at the expense of imported cases: in 81.3% of cases - in St. Petersburg; 63.3% - Leningrad; 60% - Kostroma; 52% - Volgograd; 50% - Ivanovo regions [8].

However, if you take into account the number infected with trichuriasis people (especially children under the age of 14 years) and other pathogen parasitic zoonosis Trichuris (= Trichocephalus) vulpis, epidemiological situation, reflected Romanenko N. And., Malysheva N. With. [8], would be even more sad several times, as according to all-Russian research Institute of helminthology named after K. I. Scriabin (FIGIS) only in Moscow the number of dogs exceeds 1.000.000 million individuals.

Of which thousands, especially puppies, can be infected with a pathogen parasitic zoonosis Trichuris (= Trichocephalus) vulpis and especially those breeds of dogs that were brought from more southern countries (Mexico, Egypt, Greece, & C�incurred, Ukraine, Spain, Romania, France, Italy, etc.) or regions of the Russian Federation (Rostov, Saratov, Astrakhan region. Krasnodar territory, Republic Adygea, Dagestan, Crimea, etc.) that can serve as an additional source of infection trichuriasis people, especially in large cities: Moscow, St. Petersburg, etc., as dog owners often ignore the veterinary-sanitary rules on their content [Pasechnik V. E. - 5].

The increasing spread of many parasitic diseases due to increasing tourism, trade and the importation and breeding of exotic animals, including caracals, Cougars, leopards, lions, Bush dog, African foxes Fennec Fox and endemic to Russia animals: ferrets, Amur leopard, Amur tiger, wolves, red wolves, domestication in foxes Siberia, which are the definitive hosts of different species of Trichuris, and among these species often dominates the causative agent of parasitic zoonosis Trichuris (= Trichocephalus) vulpis[4, 5, 6, 7].

It is known that to obtain the fees of helminth eggs, researchers used the following methods:

flotation, combined from spontaneously infected with trichuriasis animals [Kotelnikov G. A. - 1; Pasechnik, V. E., and others - 4].

These methods have the following disadvantages:

a) unknown species of parasite, as the CRO�e Trichuris (= Trichocephalus) vulpis in training camp can be eggs and other species and genera of helminths, parasites of domestic and wild carnivores: Thominx aerophilus (Creplin, 1839);

b) they require large expenditures of time and labor, as fees for large quantities of eggs of trichocephalus respectively, and a large amount of faeces spontaneously and in heavily infected with trichuriasis animals;

b) severe pollution fees of helminth eggs remains undigested particles of feed and especially the presence of fats from predators, which complicates research in the laboratory, so it is more appropriate to conduct in the field;

g) the number of eggs collected by these methods during spontaneous trichuriasis often very small, with weak degree of infestation.

The known method of collecting eggs through the destruction of the genitals (vulva, vagina, uterus) of female worms [Pasechnik V. E. - 2, 3].

This method has the following disadvantages:

1) it is necessary to destroy the genitals of females of worms;

2) egg collection is very heavily contaminated with the remains of the destroyed tissues of the female genital organs of females of the parasite that during the experiment causes the development of secondary bacterial microflora, the decay and destruction of helminth eggs;

3) and most importantly, in this method of collection obtain up to 100% of the fertilized eggs is impossible, since the bulk of the fertilized eggs is located in the distal part vaginae at the vulva (the vagina) and only partially in the uterus (known what in the uterus of the parasite eggs are fertilized unevenly);

4) removing the eggs from the gonads of the parasite by the above method additionally contaminate the material with a large number of unfertilized eggs;

5) in the course of experiments, researchers need to cultivate to the infective stage to study in the laboratory (biology, therapy, and epidemiology of trichuris) fees up to 100% of the fertilized eggs, and quite often the need arises (in experimental infection of laboratory animals monkeys, ferrets, rabbits, lambs, calves, etc.) to apply a dose of the infective eggs of trichuris with 1000's and even tens of thousands of eggs per animal.

Therefore, researchers for these reasons, be obtained by using these known methods egg gatherings: flotation, combined; the collection of eggs by destroying genital (= copulative) bodies of females of the pathogen parasitic zoonosis: Trichuris (= Trichocephalus) vulpis, maximum number up to 100% of the fertilized eggs of trichuris (= trichocephalus) for experiments in laboratory conditions is very difficult and often almost impossible.

The aim of the invention is the use for the first time developed a "Method of lifetime collecting fertilized eggs (in vitro) from the pathogen parasitic zoonosis: Trichuris (= Trichocephalus) vulpis", for experiments:�of steverivonia to the infective stage in the laboratory in the study of biology, therapy, epidemiology, conducting morphological studies to study the microstructure, ultrastructure of the eggs to infect laboratory animals, with the culture of eggs in the most sterile conditions, i.e. without contamination by tissue slices of the uterus, vagina, vulva and without entering secondary microflora of the genital organs and the external environment, and most importantly to receive lifetime; the most natural way (close to the conditions of life of females trichocephalus in the body of prey) to 100% fertilized eggs from living, sexually Mature females of the pathogen parasitic zoonosis: Trichuris (= Trichocephalus) vulpis.

This object is achieved in that during the collection of eggs from trichuris used designed us for the first time "Method of lifetime collecting fertilized eggs (in vitro) from the pathogen parasitic zoonosis: Trichuris (= Trichocephalus) vulpis, which is to have selection in tubes only living, sexually Mature females trichuris (= trichocephalus) Trichuris (= Trichocephalus) vulpis from the colon and caecum spontaneously infected with trichuriasis, when helminthological research methods at autopsy, wild and/or domesticated carnivorous [by K. I. Skryabin - 9] officinal in sterile isotonic solution (0.9%) sodium chloride (solutio Natrii chlorati isotonica); and the exposure tubes with females Trichuris (= Trichocephalus) vulpis at t=37.5°C-39°C for 5 hours�in the conditions of thermostat; different from the known methods egg collection: flotation combined method of destruction of the genital organs of the female worms, that:

no need for a large number of spontaneously infected with trichuriasis animals, as can be collected at autopsy several sick animals from 200 and more females Trichuris (= Trichocephalus) vulpis and, therefore, be obtained by the proposed method of collecting over 2,000 fertilized eggs (unlike methods chaprovskoye when the researcher has limited the possibility of using in the experiment a large number of animals, spontaneously infected with trichuriasis prey can be in a single copy or a small number (zoo, circus, hunting, rare and endangered species: American Bush dog, red wolves, white plains, ferrets, African foxes Fennec Fox or dog breeds: Sphynx, etc.);

sex (= copulative) bodies of females are not destroyed (not opened) for fertilized eggs from pathogen parasitic zoonosis Trichuris (=Trichocephalus) vulpis (unlike the way they collect eggs by destroying (= opening) of gonads of female worms), occurs in vivo, arbitrary, natural oviposition (= highlight) the fertilized egg alive, females Trichuris (= Trichocephalus) vulpis (unlike known�about the way they collect eggs of helminths, when opened (= destroy) genitalia female trichuris(= trichocephalus));

no pollution fees a large number of unfertilized eggs Trichuris (= Trichocephalus) vulpis (unlike the way they collect eggs by opening (= destruction) of gonads of female worms);

without pollution, the collection of eggs Trichuris (= Trichocephalus) vulpis particles of undigested food and especially fats from predators (unlike ways of collecting eggs of helminths: flotation, combined);

eliminates the need to explore a large number of faeces from spontaneously infected with trichuriasis wild and/or domesticated carnivorous to get the maximum number of fertilized (1000 and more) Trichuris eggs (= Trichocephalus) vulpis (unlike ways of collecting eggs of helminths: flotation, combined);

enough to have the experience of 200 or more living, sexually Mature females to get 2000 or more fertilized eggs Trichuris (= Trichocephalus) vulpis (unlike ways of collecting eggs: flotation, combined, in which it is necessary to investigate a large number of faeces from spontaneously infected with trichuriasis wild and/or domesticated carnivorous to collect 1000 or more fertilized eggs that almost extremely difficult, the presence in samples of feces of single eggs of trichuris);

without contamination of the collection of particles destroyed sex (= copulative) of the sa�OK (vulva, vagina, uterus): Trichuris (=Trichocephalus) vulpis (unlike the way they collect eggs: the autopsy (= destroy) genitalia female worms);

without the development of secondary microflora and thereby prevent putrefaction and destruction in the received collection of eggs of trichocephalus (= trichuris) species: Trichuris (= Trichocephalus) vulpis;

researcher (experimenter) is clean, lifetime fee of up to 100% of fertilized eggs from living, sexually Mature females of the pathogen parasitic zoonosis: Trichuris (= Trichocephalus) vulpis.

Comparative analysis of the claimed technical solution with the prototype: known methods of flotation, combined, gathering eggs when destroying the reproductive organs of females helminths, shows that the claimed method differs from the known fact that the conduct of separate (individual) has just recovered from cecum and large intestines method helminthological autopsy of wild and/or domesticated carnivorous collection of live, Mature females of parasitic zoonosis Trichuris (= Trichocephalus) vulpis in each test tube with the officinal isotonic solution (0.9%) sodium chloride (solutio Natrii chlorati isotonica) and exhibit vials with females Trichuris vulpis at t=37.5°C-39°C for 5 hours in the condition of thermostat.

After this exposure, the experimenters receive up to 100% of the net fees of eggs released in vivo, naturally, at random from the genitals (�of Ulvi, vagina, uterus) of living, sexually Mature females trichuris, since the living, sexually Mature females whipworms forced to delay arbitrarily, naturally officinal in isotonic solution (0.9%) sodium chloride and, therefore, in tubes up to 100% of the fertilized eggs of the pathogen parasitic zoonosis: Trichuris (= Trichocephalus) vulpis.

These differences allow to draw a conclusion about conformity of the proposed technical solution the criterion of "novelty". The features distinguishing the claimed technical solution from the prototype (methods egg gatherings: flotation, combined; the destruction of the female genital organs of females), not identified in other technical solutions, and therefore they provide the claimed technical solution the criterion of "substantial differences".

Examples of specific performance of the proposed method

Example 1. In Soroka (Moldova) from 12 dogs, spontaneously infected with trichuriasis, collected eggs of trichuris (= trichocephalus) by known methods (control) flotation, combined and offered by way of lifetime of collecting fertilized eggs from 200 living, sexually Mature females Trichuris (= Trichocephalus) vulpis (experience) within 5 working hours.

When conducting duties of eggs known methods of chaprovskoye (flotation on Fulleborn; combined according to Darling - 1) (control) were obtained following� results:

the number of eggs in the collection amounted to only 890 eggs, which undoubtedly scanty for the infection of laboratory animals;

in the collection there were many particles of undigested food and especially fats, which greatly hindered research and the number of eggs in fees;

infected dogs it was necessary for the taking of fresh faeces in large numbers periodically fix that took considerable working time (over 45 minutes);

collected methods of flotation and combined eggs trichuris needed to be rinsed from salt solutions at least three times, which reduced the number of eggs in training camp, as part of the eggs were lost in the washing process;

the amount of faeces from infected dogs sometimes had very little for the production of samples for the collection of eggs of trichocephalus (= trichuris) methods: flotation, combined 5 hours of working time.

At the same time put experience to gain 200 living, sexually Mature females of eggs of trichuris (= trichocephalus) on the proposed "Method of lifetime collecting fertilized eggs (in vitro) from the pathogen parasitic zoonosis Trichuris (= Trichocephalus) vulpis" (the experience).

200 living, sexually Mature females Trichuris (= Trichocephalus) vulpis received from investigated at autopsy (from the colon, and cecum) spontaneously infected with trichuriasis dogs were placed in each test tube with about�icinalis isotonic solution (0.9%) sodium chloride (solutio Natrii chlorati isotonica) were exposed at t=a 37.5-39°C for 5 hours in the condition of thermostat (the experience).

After 5 hours the tube was removed from thermostat and summarized the experimental results for the proposed Method in vivo collection of fertilized eggs (in vitro) from the pathogen parasitic zoonosis Trichuris (= Trichocephalus) vulpis and in comparison with the results of verification: the collection of eggs by known methods: flotation, combined:

estimate the number of pending fertilized eggs alive, females Trichuris (= Trichocephalus) vulpis that according to our results amounted 4570 eggs (experience), but controlling for known methods of collecting flotation, combined - 890 eggs that in the latter case, certainly not enough to carry out experiments on the infection of laboratory animals;

no need when we proposed the Method of lifetime of collecting fertilized eggs (in vitro) from the pathogen parasitic zoonosis Trichuris (= Trichocephalus) vulpis" (experience) in a large number of spontaneously infected with trichuriasis animals, as can be collected at autopsy several sick animals from 200 and more females Trichuris vulpis (in contrast to the known methods egg collection: flotation, combined, (control), when the researcher has limited the possibility of using in the experiment a large number of animals, spontaneously infected with trichuriasis prey can be in the singular or in small numbers (zoo, circus,rare and endangered species). In our example, 12 dogs, and the living, sexually Mature females Trichuris (= Trichocephalus) vulpis - 200);

there is contamination on the proposed "Method of lifetime collecting fertilized eggs (in vitro) from the pathogen parasitic zoonosis Trichuris (= Trichocephalus) vulpis" (experience) particles of undigested food and especially fats from predators (unlike the known methods of collecting eggs of helminths: (control): flotation, combined);

eliminates the need for a study of a large number of faeces from spontaneously infected with trichuriasis wild and/or domesticated carnivorous, with the aim of obtaining the maximum number of (experience) fertilized (2000 or more) of Trichuris eggs (= Trichocephalus) vulpis (unlike the known methods of collecting eggs (control) worms: flotation, combined).

The results showed that when collecting eggs known methods: flotation, combined (control) had the following disadvantages:

in training camp there were many remnants of digested food, especially fats;

b) sampling of faeces individually from the rectum took up much of my time, since it was necessary to record each time the dogs;

b) samples of feces from each dog had to re-take, since it is not always the amount of excrement was enough for research;

d) the number of eggs of trichuris (= trichocephalus) in 1 g of samples p�and research were negligible.

Therefore, under field conditions, these known methods of collection (flotation, combined) Trichuris eggs (= Trichocephalus) vulpis may be used if the number of eggs in collecting enough for experiments, but during laboratory tests:

1) culturing of eggs before the formation of infective larvae;

2) infection of experimental animals when it is necessary to infect laboratory animals with increasing doses of infective material: 500, 1000 and even tens of thousands of parasitic eggs per animal, the number of eggs collected is clearly insufficient.

This example clearly shows the advantage of the proposed Method in vivo collection of fertilized eggs (in vitro) from the pathogen parasitic zoonosis Trichuris (=Trichocephalus) vulpis".

Example 2. In the study of the large intestine and the cecum at autopsy 9 spontaneously infected with trichuriasis dogs from private owners, stray dogs, dogs, shepherd dogs, 4 foxes in the Republic of Moldova were collected for the experiment in two ways:

1) the proposed method of collection (experience) of fertilized eggs, which were collected only living, sexually Mature females of the pathogen parasitic zoonosis: Trichuris (= Trichocephalus) vulpis;

2) by a known method of collection and the control of helminth eggs: the destruction of the gonads of female Trichuris vulpis, which are collected for p�of Ogadenia fees of helminth eggs in a row all discovered at autopsy parasites and, as a rule, collected in the period helminthological autopsy of prey can be Mature and immature (= young) female Trichuris (= Trichocephalus) vulpis.

Therefore, in many cases in the cultivation of eggs of trichuris to the infective stage in the laboratory, researchers are faced with many challenges, namely the main of them are:

the development of the putrefactive process, due to the presence of charges, and hence in the culture of eggs remains of the ruined tissues of the genital organs of females Trichuris; the availability of secondary bacterial microorganisms, parasitic fungi, which are always written from the environment, contamination of the collection of particles of undigested food, fat; the presence of a large number of unfertilized eggs (a result of the destruction of the gonads not only Mature, but also immature (= young) females Trichuris).

Collected 450 females Trichuris (=Trichocephalus) vulpis were used to obtain the fees of eggs in two ways:

1st (experience) of the claimed method, i.e. individual (in a separate vial) egg collection, only from every living, sexually Mature females Trichuris (= Trichocephalus) vulpis; active, lively, 225 Mature females trichocephalus, lifetime, arbitrarily, naturally lay their fertilized eggs in test tubes with officinal isotonic solution (0.9%) sodium chloride (solutio Natrii chlorati isotonica) in perio� exposure tubes with females Trichuris (= Trichocephalus) vulpis at t=37.5°C-39°C for 5 hours in the condition of thermostat.

And the 2nd (control) in a known manner, the collection of eggs by destroying the genitals (vulva, vagina, uterus) 225 females Trichuris (= Trichocephalus) vulpis collected in a row of the small intestine and the cecum, studied at autopsy wild (foxes) and domestic (dogs) predatory.

In the next selected row 2500 Trichuris eggs (= Trichocephalus) vulpis received:

(a) proposed method, the lifetime of collecting fertilized eggs (1st experience);

b) in a known manner of collection of eggs by destruction of gonads of female worms (2nd control).

Further compared the results obtained by these methods of data collection - fertility of eggs, and to this end:

(a) cultured eggs of Trichuris (= Trichocephalus) vulpis pathogen parasitic zoonosis (= trichuriasis) in a thermostat at t=20°-25°C for 26 days before stage infective larvae;

b) examined eggs of trichuris Trichuris vulpis under the microscope to compare our proposed "Method of lifetime collecting fertilized eggs (in vitro) from the pathogen parasitic zoonosis Trichuris (= Trichocephalus) vulpis (1 year experience) and known "Method of collecting eggs, when researchers destroy the genitals of females trichuris (= trichocephalus): the uterus, vagina, vulva, the causative agent of parasitic zoonosis Trichuris (= Trichocephalus) vulpis" (2nd control).

The results showed that in training camp 2500 Trichuris eggs (= Trichocephalus) vulpis by a known method, whereby on opening�s (= destroy) genitalia female worms: vulva, vagina, uterus (2nd control):

developed for 26 days of observation 37% (930) of fertilized eggs;

the collection was 63% (1570) unfertilized eggs, which greatly hampered the study;

all the eggs were contaminated with a great number of particles of the destroyed tissues of the genitals (vulva, vagina, uterus) female Trichuris (= Trichocephalus) vulpis;

a significant portion of working time in the period of research known method of collection through the destruction of gonads of female Trichuris vulpis took the sample from unfertilized fertilized eggs in the collection;

in collecting the evolved bacterial microflora and the parasitic fungi causing rotting eggs, which greatly hampered the experiment.

While the proposed Method (1st experience) the collection of fertilized eggs (in vitro) from the pathogen parasitic zoonosis Trichuris (= Trichocephalus) vulpis" 2500 eggs pathogen parasitic zoonosis: Trichuris (= Trichocephalus) vulpis:

in the collection of eggs was absent the particles of destroyed tissues of the genitals (vulva, vagina, uterus) female Trichuris (= Trichocephalus) vulpis;

in the proposed method of collection was missing unfertilized eggs;

up to 100% of cases 2500 fertilized eggs of Trichuris (= Trichocephalus) vulpis evolved without the presence of secondary bacterial microflora.

Thus, the results of culturing eggs collected on our "Way of lifetime sat�RA (in vitro) fertilized eggs (1st - experience), confirmed the data obtained using the proposed "Method of lifetime collecting fertilized eggs (in vitro) from the pathogen parasitic zoonosis: Trichuris (= Trichocephalus) vulpis" that were revealed for the first time.

Using the proposed "Method of lifetime collecting fertilized eggs (in vitro) from the pathogen parasitic zoonosis: Trichuris (= Trichocephalus) vulpis" from the wild and/or domesticated carnivorous provides in comparison with known methods: (3) flotation, combined; the collection of eggs by destroying the female genital organs female worms (vulva, vagina, uterus), with the following benefits:

known species of the pathogen: Trichuris vulpis and/or Thominx (= Capillaria) aerophilus (unlike the known methods of collecting eggs of helminths: flotation and combined, when an unknown species of parasite);

shortens the time and reduces the complexity of the research exception:

a) the need for studies of a large number of faeces from spontaneously infected with trichuriasis wild and/or domesticated carnivorous to collect the maximum number of fertilized eggs Trichuris vulpis (in contrast to the known methods egg collection: flotation, combined);

b) the need to conduct the autopsy of the female genital organs female Trichuris (= Trichocephalus) vulpis (in contrast to the known method of collection of eggs destruction of the vulva, vagina�, the uterus of worms);

b) necessary in the preparation and use of various chemical solutions for collecting eggs of Trichuris vulpis (unlike the known methods of collecting eggs of helminths: flotation and combined);

g) required in the centrifuge (in contrast to the known methods of collecting eggs of helminths: flotation, combined);

eliminates contamination of the egg collection scraps of tissue particles genitalia female Trichuris (= Trichocephalus) vulpis (unlike the way the destruction of the gonads of female worms);

eliminates contamination of collecting eggs from Trichuris (= Trichocephalus) vulpis particles of undigested food and especially fats from predators (dogs, foxes, etc.) (unlike ways of collecting eggs of helminths: flotation, combined);

eliminates decay, the development of secondary bacterial infection in the culture obtained by our proposed method in vivo collection of fertilized eggs from living, sexually Mature females of the pathogen parasitic zoonosis species of Trichuris (= Trichocephalus) vulpis;

method of lifetime collecting fertilized eggs (in vitro) from the pathogen parasitic zoonosis Trichuris (= Trichocephalus) vulpis from wild and/or domesticated carnivorous easily implemented in the laboratory;

the proposed method provides a clean collection (no scraps of particles of the tissues of the genital organs of the female (the vulva, vagina, uterus) - in contrast to the known method SRB�and eggs: the destruction of the gonads of females; without particles of undigested food and fat - in contrast to the known methods of collection of helminth eggs), with up to 100% of the fertilized eggs and in large enough quantities (if necessary, up to several thousand eggs and from the known species of helminth: Trichuris vulpis - unlike the known methods egg collection: flotation, combined) obtained in a natural way, arbitrary, lifetime oviposition alive, females pathogen parasitic zoonosis: Trichuris (= Trichocephalus) vulpis (unlike post-mortem (after death of the parasite!) method of collecting eggs of helminths - the destruction of the gonads of females), and is intended for use in experimental parasitological studies in the laboratory.

Sources of information

1. Kotelnikov G. A. Helminthological research animals and the environment. // The directory. - M. - spike - 1983 - 208.

2. Pasechnik V. E. Cross-contamination trihotsefalami prietary dogs and sheep in Moldova. Byull. All-Union Institute of helminthology. - M. - 1984. - Vol. - 37. - P. 57.

3. Pasechnik V. E. Diss. the candidate of veterinary Sciences. - M. - VYGIS. - 2000. - Pp. 124-125.

4. Pasechnik, V. E., Uspensky V. A., mikulec Y. I., Zhdanov V. Yu. / / guidelines for the diagnosis, prevention and combating helminthiasis circus animals. M. Ed. RAAS. (=RAAS). - 2008. - 50 S.

5. Pace�nick V. E. The first death from trichuriasis most valuable breeds of dogs registered in Russia. // Abstracts of scientific conference. "Theory and practice of prevention of parasitic diseases". - M. Ed. RAAS, 2010. Vol., 11. - S. 351-353.

6. Pasechnik V. E. Distribution and species composition of helminths and coccidia in brown bears in the Russian Federation. // Russian journal of Parasitology. M. Ed. RAAS. 2010, No. 1. P. 15-21

7. Pasechnik V. E. Helminthoses Squad Camivora (Prey) in the Moscow circus "big top". // Abstracts of scientific conference. "Theory and practice of prevention of parasitic diseases". - M. Ed. RAAS. - 2011. - Vol. 12. - P. 377-379.

8. Romanenko N. And., Malysheva N. With. Ecological framework for prevention of parasitic diseases. - M., 2006. - 327 p.: ill.

9. Skryabin, K. I. Method complete helminthological dissection of vertebrates, including humans. // Ed. 1-St Moscow state University. - M. - 1928 - 36 p.

10. Skryabin K. I., Petrov A. M. Trichocephalus carnivorous. // The basics of veterinary nematology. M. Ed. Ear.- 1964. - S. 476-478.

11. Sakano, T.; hamamoto's K.; Kobayayashi Y.; Sakata Y.; Tsuji, M.; Usui T. Visceral larva migrans causet by Trichuris vulpis. // Archves of Disease in Childhood. - 1980. - V. 55. - N 8. - P. 631-633.

12. Singh S, Samantaray J. C., Singh, H., Das G. B., Verma I. C. Trichuris vulpis infection in an Indian tribal population. // Journal of Parasithology. - 1993. - 79 (3). - P. 457-458.

Method of collecting fertilized eggs in vitro) from the pathogen parasitic zoonosis: Trichuris (=Trichocephalus) vulpis, which consists in selecting only the living, sexually Mature females Trichuris vulpis from the colon, caecum spontaneously infected trihotsefalami in the study helminthological methods at autopsy of wild and domestic prey in individual tubes with the officinal isotonic solution (0.9%) sodium chloride (solutio Natrii chlorati isotonica) and exposure tubes with females Trichuris vulpis at t=37.5°C - 39°C for 5 hours in the condition of thermostat.



 

Same patents:

FIELD: medicine.

SUBSTANCE: after thymomegalia has been excluded, tissue specimens of three-day-old mature newborns are studied to evaluate areas of inflammation changes in points in the placental umbilical cord (A), in the foetal placenta (B), in the maternal placenta (C), in extraplacental membranes (D); then thymomegalia is predicted by a discriminant equation: DE=-0.350×A-1.176×B-1.690×C-1.203×D, wherein DE is a discriminator function with a threshold equal to - 15.00. If DE is equal to or more than the threshold, the absence of thymomegalia is predicted; if D is less than the threshold, thymomegalia is predicted, whereas the score is taken at: (A) - 1 point - no inflammation, 2 points - amnionitis, 3 points - leukocytic infiltration in the Wharton's jelly, 4 points - phlebitis, 5 points - arteriitis, 6 points - a combination of two or more areas of inflammation: in blood vessels or in vessels and in the Wharton's jelly, (B) - 1 point - no inflammation, 2 points - chorioamnionitis, 3 points - villusitis, 4 points - vasculitis, 5 points - intervillesitis, 6 points - a combination of two or more areas of inflammation, (C) - 1 point - no inflammation, 2 points - villusitis, 3 points - vasculitis, 4 points - intervillesitis, 5 points - deciduitis, 6 points - a combination of two or more areas of inflammation, (D) - 1 point - no inflammation, 2 points - amnionitis, 3 points - chorioamnionitis, 4 points - deciduitis, 5 points - choriodeciduitis, 6 points - a combination of two or more areas of inflammation.

EFFECT: enabling the prediction of thymomegalia in the three-month-old mature newborns suffered prenatal influenza B complicated by placentitis.

2 ex

FIELD: medicine.

SUBSTANCE: after the ablation of the spleen, its weight is determined, the average area of a marginal zone of the spleen in histological cuts with coloration with hematoxylin and eosin is measured morphometrically. The obtained values are used to calculate the weight of the marginal zone and, if its values are ≤1.9 g, a favourable prognosis for the course of aplastic anaemia is made, if its value is >1.9 g, an unfavourable course of the disease is predicted.

EFFECT: prognosis of the aplastic anaemia course after splenectomy independent on the severity of the disease with the possibility to approach therapeutic treatment in a differential way.

2 ex

FIELD: medicine.

SUBSTANCE: gastric content pH is dynamically estimated with the use of a nasogastric tube; the total gastric content pH is measured and the efficacy of the antisecretory preparations is estimated for one day, or a longer period of time if needed, every 3 hours. The pH value is measured by means of an analogue display unit on an analogue scale at a pitch of 1.0 within 1.0 to 12.0 for 15 s as shown by discolouration, with matching to the analogue scale. The acid production suppression is considered to be effective at pH of more than 4.0 after planned surgeries, and more than 6.0 after emergency surgeries. If the acid production suppression is found to be ineffective, the antisecretory preparation is supposed to be changed or increased in dose.

EFFECT: method enables measuring the gastric content pH and estimating the efficacy of the antisecretory preparations in the patients in need thereof over a short period of time.

2 dwg, 2 tbl, 3 ex

FIELD: medicine.

SUBSTANCE: method of neurosyphilis diagnostics includes the microscopic analysis of cerebrospinal fluid samples, with carrying out edge dehydration of the cerebrospinal fluid samples, and their microscopic analysis being carried out in a polarised light; in case of the detection of anisotropic structures in the form of dendrites or spherulites inside which oval-shaped formations, containing lipids, are located, an early form of neurosyphilis is diagnosed; and in case of the detection of a multitude of ovals, aggregated in the form of balls, included in the anisotropic structures and/or located separately, late meningovascular neurosyphilis is diagnosed. The invention task is to obtain objective criteria for the diagnostics of neurosyphilis, provision of early, including pre-clinical diagnostics of the disease, reduction of the terms for obtaining data, reduction of costs for carrying out analyses.

EFFECT: in a number of cases the method is the only one which makes it possible to obtain the valid criteria for diagnostics in the form of a picture of the brain structures destruction, which makes it possible to diagnose neurosiphilis confidently and prescribe specific therapy in due time.

4 dwg, 3 ex

FIELD: medicine.

SUBSTANCE: group of inventions relates to medicine, cosmetology, production of food products, vitamins, food supplements, drugs and describes versions of device for realisation of non-invasive potentiometric determination of oxidant/antioxidant activity of biological tissues, which includes device for measuring potentials and double-sided electrode, made in form of plate with similar working surface, covered with electricity-conducting gel, containing mediator system. Electrodes are fixed on biological tissue in such a way that one working surface, playing role of measuring electrode, is in direct contact with biological tissue via gel, second working surface pale role of comparison electrode. Electrodes contact with each other via gel, with oxidant/antioxidant activity being determined by formulae with application of difference between final and initial potentials.

EFFECT: simplification, as well as increase of accuracy and reliability of determination, is achieved.

14 cl, 3 tbl, 4 dwg

FIELD: medicine.

SUBSTANCE: testicular germ cells are measured quantitatively. That is ensured by 50-day oral administration of selexen and ascorbic acid into male white rats in doses 1.5 and 500 mg/kg of animal's body weight respectively once a day. 14 days later, administering the selenium-containing biocomplex is accompanied by the 30-minute daily exposure to microwave radiation at 42 GHz (λ=7.1 mm) for 30 days. Once the experimental exposures are completed, the corrective properties of the biocomplex as having an effect on the morphofunctional state of epididymal sperm cells are assessing by formula: MFSI=A+B, wherein MFSI is a morphofunctional state index, A is a portion of normal sperm cells in relation to the reference, and B is a portion of moving sperm cells in relation to the reference. If the MFSI value is 1.3 or more, the spermatogenesis correction is considered to be ineffective, while the MFSI value being more than 1.3 shows the effective spermatogenesis correction if exposed to microwave radiation.

EFFECT: invention enables assessing the spermatogenesis correction efficacy with underlying administration of the biocorrector.

2 tbl, 3 dwg, 3 ex

FIELD: medicine.

SUBSTANCE: test tray comprises a case 1 made of an optically transparent material. From one end face, the case 1 has a stop plug 2 with a hole 3, a connecting pipe 4 threaded to connect to either a haemofilter 5, or a cap 7. The case 1 comprises a movable piston 8 connected by a rod 10 to a handle 11. Electrically supplied electrodes 12 are arranged on surfaces of the stop plug 2, piston 8 and on the inner surface of the case 1. The electrodes 12 are connected to contact groups of a device - a laser analyser - through conductors 13, 14. What is disclosed is an alternative version of the structural embodiment of the test tray.

EFFECT: sterile measurement of the colloidal fluid sample.

17 cl, 5 dwg

FIELD: medicine.

SUBSTANCE: technique involves the three-stage diagnosis of all the patients suffering tumour diseases of various localisations. The first stage involves CT-densitometry performed every 6 months; if the CT-densitometry shows sites with varying spine bone density by 30% and more, the second diagnostic stage that involves a transpedicular biopsy is initiated. If the biopsy material appears to contain no tumour material, the third diagnostic stage starts with F-18 positron-emission tomography (PET-CT).

EFFECT: improving the early diagnosis of the spinal tumours.

1 ex

FIELD: medicine.

SUBSTANCE: clinical assessment of the oral mucosa state is ensured by examining non-stimulated oral fluid or swabs. That involves determining five parameters: content of yeast-like fungi Candida in the yeast or mycelia form (1), concentration of secretory immunoglobulin A (SIgA) (2) and lysozyme (3), emission light sum (S) 5 minutes before chemoluminescent examination (4); the Wood-beam beam luminescent examination covers marginal portions of the gum and apexes of interdental papillas, buccal mucosa within dental occlusion, dorsal surface of the tongue within the thread-like processes, and performing morphological examination of the multilayer squamous epithelium of the buccal mucosa along the dental occlusion line (5). The derived results enable diagnosing the absence of pathogenic microflora and oral mucosa pathology, Candida carriage or chronic oral candidiasis in the mycelia or yeast form in the form of hyperkeratosis or leukokeratosis.

EFFECT: using the invention enables increasing the differential diagnostic accuracy for keratolytic processes in the form of white manifestations.

10 dwg, 5 ex

FIELD: chemistry.

SUBSTANCE: method includes selection, and crushing of biomaterial, two-stage extraction of pesticides with n-hexane, purification of biomaterial from coextractive substances with concentrated sulphuric acid, formation of concentrate of n-hexane extract of pesticides, drying, sample formation by dissolution in 0.5-1 ml of n-hexane and carrying out gaschromatographic identification.

EFFECT: invention is characterised by higher effectiveness and accuracy of research and can be used in biology, ecology, medicine for gaschromatographic identification of organochlorine pesticides, namely α-HCCH, β-HCCH, γ-HCCH, DDT, DDD, DDE, in various biomaterials, such as lipids of internal organs and tissues, blood, milk, bird feathers.

2 cl, 1 dwg

FIELD: medicine.

SUBSTANCE: invention refers to medicine and biology, namely to a method for additional electron-dense contrast enhancement of acid groups of biomolecules accompanying the histochemical detection of sodium cations in pulmonary and tracheal cell and tissue ultrastructures. Substance of the method consists in fixing tissue slices, washing the surface in bidistilled water, placing into a diluted agent containing 4% osmium tetraoxide 1 ml and 2% potassium hexahydroantimonate 8 ml. The tissue slices are stained for 4 hours while stirring strongly and washed in bidistilled water. The tissue slices are further prepared by slicing thinner and dehydrated; the semi-thin and ultrathin slices are produced and studied by a transmission electron microscope, which is followed by computer processing to detect diffuse selective staining of the acid cell ultrastructures and intracellular substance.

EFFECT: using the declared method enables the additional electron-dense contrast enhancement of the acid groups of biomolecules accompanying the histochemical detection of sodium cations in the pulmonary and tracheal cell and tissue ultrastructures.

5 dwg, 1 ex

FIELD: electricity.

SUBSTANCE: preparation method of a dielectric specimen for investigation on a focused-beam electronic microscope of its micro- and nanostructure involves application of a current-carrying coating onto the specimen surface and provision of electrical contact of the specimen coating with the current-carrying object table. The current-carrying coating is applied by wetting of the specimen surface with a solution of hydrophilic non-evaporable non-flammable non-toxic current-carrying ionic liquid in the form of tetrachloroferrate of N-decylpyridinium in acetone and further drying of the specimen in the air till complete removal of a volatile component.

EFFECT: prevention of accumulation of electrical charges on the surface of dielectric specimens.

2 dwg

Sampling device // 2556851

FIELD: oil and gas industry.

SUBSTANCE: sampling device contains a main pipeline, a sampling section joined with the main pipeline with a possibility of sampling with coverage of liquid flow cross section, a sampling tap and a manometer. The sampling tap is designed as housing and bushing which is rigidly joined with a handle, and in a starting position the bushing shuts a drain hole of the housing, and the handle can move together with the bushing, opening the drain hole of the housing in working position. The hollow cylinder with the central channel is installed in the main pipeline, and the hollow cylinder from side of liquid flow movement is fitted with the input cone tapering the flow and the output cone expanding the flow on the other side of the hollow cylinder. The whirler fitted inside with tangential channels is installed in the central channel of the hollow cylinder from the input cone. The bushing of the sampling tap covers hermetically from outside the housing with the drain hole and can move restrictedly in axial direction with reference to the housing. The housing has the first and second external cylindrical grooves and the cutting spring lock ring is located inside. The bushing is fitted with internal ring sampler.

EFFECT: improvement of quality of liquid sampling, improvement of operational reliability of the sampling tap and increase of level of correctness of identified process parameters of wells and layers according to analyses of samplings.

2 dwg

FIELD: biotechnology.

SUBSTANCE: method comprises the steps: collecting from the bile ducts of liver of domestic and/or wild animals infected with fasciolas of only live adult F. hepatica. Placement them into individual tubes with filtered and centrifuged bile diluted with isotonic solution of sodium chloride 1:1. Exposure of tubes at t = 38-39°C if F. hepatica is from cattle, and t = 39-40°C if F. hepatica is from sheep and/or goats, under conditions of thermostat for 5 hours. Subsequent washing eggs in isotonic solution of sodium chloride.

EFFECT: invention enables to obtain up to 100 percent of fertilised eggs of Fasciola of species Fasciola hepatica and can be used for study in the laboratory or field experiments in solving fundamental and applied scientific tasks in the field of epizootiology, treatment and prevention of fascioliasis of domestic or wild animals.

2 ex

FIELD: agriculture.

SUBSTANCE: invention relates to the field of agricultural machinery industry. The device for sampling chopped straw from grain combine harvesters comprises collectors, a retainer, a bracket and a control lever. The collectors are arranged in rows, the number of which in the transverse direction is determined by the header coverage and the selected number of the studied areas, and in the longitudinal - replication of sampling. The collectors are interconnected in longitudinal rows by means of flexible connections equal in magnitude, so that the total length of the row does not exceed the distance to the ground mass descent area on the stubble. The first collectors in each longitudinal row have easily removed connections on the holes in the rod-float leveller which is located perpendicular to the direction of the combine movement and retained by the retainer driven by the control lever.

EFFECT: invention provides a boundary separation of the comparable areas in sampling and reduction of the probability of failure of experimental equipment.

2 cl, 1 dwg

FIELD: instrumentation.

SUBSTANCE: invention relates to the method of analysis of a set of ferromagnetic particles. The method is characterized by that the particles of the named set are levelled in such a way that each of the named particles is oriented practically in the same direction. Then the particles of the named set are fixed in this aligned direction and the internal areas of the named particles levelled in such a way are uncovered. After that the nature of the alloy comprised by each of the named particles is identified, the named particles are grouped by categories depending on their nature and the metallurgical structure and chemical composition of one or more of the named particles in each category are determined.

EFFECT: improvement of accuracy and reliability of the analysis of ferromagnetic particles.

14 cl, 6 dwg

FIELD: instrumentation.

SUBSTANCE: invention relates to forecast of ageing processes of the synthetic polymer materials (SPM) depending on duration of their operation or storage. Analysis of the volatile organic compounds (VOC) migrating from SPM is performed by active sampling for sorbent, with further thermal desorption and gas chromatographic analysis. Forecast of the ageing processes of the material and estimation of toxicity of the gas discharge are performed as per dynamic of the qualitative and quantitative composition of gas discharge components in SPM initial state, and during artificial climatic thermal-humidity ageing. Dynamics analysis of the total gas discharge (ΣT) from each material is performed for all substances migrating from studied SPMs. Change of toxicity is estimated and forecast of the material ageing is performed as per developed indices of total gas discharge (ΣT) and as per hygienic index P=(ΣTinitial/ΣTn)/V, where Tinitial and Tn are indices of toxicity of gas discharge of each substance in initial state and after ageing, respectively, and ΣTinitial and ΣTn are total indices of toxicity of gas discharge of all components of SPM in initial state and after ageing, V is duration of ageing (year, month).

EFFECT: invention ensures high accuracy of the method of VOC qualitative and quantitative composition determination in gas discharge during materials ageing and the analysis results repeatability.

3 tbl

FIELD: physics, acoustics.

SUBSTANCE: group of inventions relates to an apparatus for irradiating a sample with focused acoustic energy, a device which is part of said apparatus, a cartridge for said device and a method of irradiating a sample with focused acoustic energy. The apparatus comprises a device, a cartridge, a completely solid-state connector and a source for generating acoustic energy. The cartridge has a chamber for receiving a sample, and the completely solid-state connector provides a completely dry coupling of acoustic energy between the source and the cartridge. The device and the cartridge are adapted for inserting the cartridge containing a sample into the device and are separable, and the focused acoustic energy is focused high-intensity ultrasound. The device has a source for generating acoustic energy and the cartridge has a chamber for receiving a sample.

EFFECT: improved sample processing.

17 cl, 24 dwg

FIELD: motors and pumps.

SUBSTANCE: simulation device contains an oil batcher, a dispersion chamber and a lubricant oil decomposition chamber (1). At the air outlet downstream the chamber the diffuser (2) is located. On the chamber the heater (3) with the thermocouple (4) and the thermorelay (5) is installed. The device includes the air duct (6) supplying the pumped-over hot air into the lubricant oil decomposition chamber connected through the manometer (7) to the air compressor (8). The device contains the cylinder (13) filled with ultra-pure nitrogen, (23, 24) the sealed gage tank with air cavity with oil and a cover for oil filling, with the oil pipeline connected to it through the gas pipeline with the regulator (12), the adapter (11) and cap nuts. The gage tank (9) is connected through the adapter (11) with cap nuts (20, 21) to the measured capillary (15) in a cooling jacket (16) with circulating water through the thermostat with the pump (18) and radiators, attached to the decomposition chamber by means of the cap nut (22) and the sealing cone (25). Also the device comprises the additional chamber (26) screwed to the main decomposition chamber of (1) coaxially and sealed with a gasket (27), with the rod with a flywheel (17) installed inside, with threaded and non-threaded parts. Meanwhile the threaded part is implemented with a possibility of movement in the internal washer with a thread (28) for adjustment of the decomposition chamber volume and change of conditions of simulation of oil concentration, while the non-threaded part is sealed in a gland with graphite seal (29).

EFFECT: improvement of accuracy of simulation of composition of oil decomposition products in aviation gas-turbine engines.

1 dwg

FIELD: aircraft engineering.

SUBSTANCE: invention relates to aircraft cabin air samplers, gadgets for analysis of admixtures in aircraft cabin air samples for analysis of concentration of contaminants in aircraft air conditioning systems and for determination of the composition of harmful admixtures and dangerous concentrations of gases and vapours in air. This sampler comprises evacuated vessel as air consumption booster, absorption cartridge with sorbent-concentrator composed of sharpened steel tube with plugs of glass wool at tube ends, with side bore in said tube filled with sorbent and glass wool. Evacuated vessel is composed of cylindrical case with inlet and outlet pipes fitted at case ends. Outlet pipe is provided with tube of vacuum rubber and metal plug to be fitted in the tube free end after evacuation. Inlet pipe is welded to the case end and features ID larger than that of the case inlet and internal thread. Aforesaid absorption cartridge with sorbent-concentrator is fitted in said inlet pipe and, partially, in evacuated vessel used also as a sampler of admixtures not absorbed by concentrator. Locking device composed of the tube with air sample passage inlet is screwed via seal ring in evacuated vessel surface neck to tight fit. Cover with stiffness ribs and rubber washer is secured to said locking device and aligned therewith. Lever with triangular cam at the end is articulated with the device on opposite side from the cover attachment side to lift said cover to unseal the system and to bleed air. Said lever and cover are secured to be revolved in one plane relative to axles of rotation and attachment. Springs secured from one side to locking device case and to cover stiffness ribs on opposite sides. This makes said cover opened and cover closed at lowered lever and cover located at lever can outer leg. Lever shifted, cover goes up to unseal the system and to bleed air.

EFFECT: higher sampler sensitivity, accuracy of analysis, accelerated in-flight experiment.

3 dwg

FIELD: automatical aids for sampling liquids.

SUBSTANCE: system for sampling and delivering filtrate has filter submerged into tested medium and connected with collecting tank and vacuum pressure source which is connected with top hole of collecting tank by means of pneumatic pipe. System has sample receiving tank connected with collecting tank and control unit which has first output to be connected with vacuum pressure source. Collecting tank has two separated chambers - washing chamber and dispatching chamber. Lower hole of washing chamber has to be lower hole of collecting tank and side hole of dispatching chamber has to be side hole of collecting tank. Floating valve is installed inside washing chamber to shut off lower and top holes. Filter is connected with lower hole of collecting tank through sampling pipe. Side hole of collecting tank is connected with lower hole of tank for receiving samples through sampling pipe. Flow-type sensor and check valve are installed inside transportation pipe. Output of flow-type sensor is connected with input of control unit; second output of control unit is connected with control input of analyzer.

EFFECT: improved precision of measurement of sample ion composition; prolonged service life of filter.

1 cl, 1 dwg

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