Method for determining expediency of immunoassay in animal-breeding workers

FIELD: medicine.

SUBSTANCE: chronic infectious-inflammatory diseases (CIIDs) are diagnosed. Clinical blood analysis and bacteriological tests are conducted. A sensibilisation index (SI) and an immune responsiveness index (IRI) are calculated; total microbial count per 1 m3 of the working space air is measured, and the total microbial number (TMN) is derived. If the TMN is less than 500 CFU/m3 with no CIIDs diagnosed accompanied by the SI of less than 1.08 standard units and the IRI of less than 13 standard units, the immunoassay is considered to be inadvisable. If the TMN falls within the range of 500-2,500 CFU/m3 with one CIID diagnosed accompanied by the SI from 1.08 to 1.3 standard units and the IRI from 13.1 to 15.7 standard units, the immunoassay with the first-level tests seems advisable. Whereas the TMN exceeding 2,500 CFU/m3 with at least two CIIDs accompanied by the SI of 1.4-1.5 standard units and the IRI of 15.8-18.3 standard units, the immunoassay with the second-level tests is thought expedient.

EFFECT: invention enables detecting the workers in need of further examination for the purpose of timely immune correction in the setting of mass routine examinations.

1 tbl, 3 ex

 

The invention relates to medicine, in particular to occupational hygiene, occupational pathology and immunology, and can be used to address the question of the feasibility of an immunological examination of workers of animal husbandry.

Specifics of technological processes in agriculture does not exclude the unfavorable impact on the body work of the complex production factors: unfavorable microclimate, dust, air pollution, high bacterial and fungal contamination of air in the working area, etc. [the Effect of microbial contamination of the air on the mucous membranes of the nose and throat in workers of the poultry industry / L. M. Mesyagutovo, A. B. Bakirov, G. A. Yanbukhtina, A. I. Sleptsova // Public health and health care. - 2011. - No. 4. - S. 37-41].

The combination of prolonged exposure to adverse factors of the working area, even at the level of maximum allowable concentration, increases the risk of formation of pathological conditions of various organs and systems. Basic mechanisms of action on the organism of production factors lead to changes in metabolic processes that are implemented at the molecular and cellular levels. Very relevant today are the issues of organization of medical provision in the village. This is due to both objective�and causes (world urbanization trend, the relocation of residents in the city), and specific for the Russian Federation. This and the reduction of financing of medical institutions, particularly in rural areas; and inadequate staffing and universal elimination of low-power rural health clinics and rural health posts in remote areas, which clearly limits the accessibility to professional care workers in the livestock complex. The above allows to conclude that the level of morbidity level of the population for medical care can be several times lower than the incidence of infection, supplemented with preventive examinations. In terms of occurrence and distribution of real and not hidden, as before, unemployment population health has become a factor of selection and occupational suitability. The latter leads to the fact that the economically active population for the purpose of maintaining employer before a certain professional image has drastically reduced the appealability for medical aid. Even greater rates being reduced uptake of this form of social protection of the population, as a temporary disability.

To improve the quality of medical services to the population engaged in agricultural production through improved methods have�management diagnostic and treatment process through effective forecasting and early diagnosis of diseases, caused by professional activities, especially in agriculture. Timely detection working, in need of a deeper study of the immune system, contribute to the formation of rational schemes of organization of health improvement activities.

A good measure of the health of the person continues to be the study of the peripheral blood, in which the dynamics is a reflection of the multifactorial external and internal processes that affect the body examined. Because certain combinations of indicators hemogram reflect the integral characteristics of homeostatic systems of the body, forming nonspecific adaptation reactions, diagnostic and prognostic capabilities integrated leukocyte indexes are currently assuming increasing importance [Ostrovsky, V. K., Mashchenko A. V., Angolina D. V., Makarov S. V. blood counts and leukocyte index of intoxication in assessing the severity and determining the prognosis of inflammatory, purulent and purulent-destructive diseases // Clin. lab. diagnosis. - 2006. - No. 6. - Pp. 50-53; Speransky, V., I. I. Dmitriev, P. M. Zaripova Immunological informative leucocythemia // Klin. lab. diagnosis. - 1999. - No. 12. - P. 6-7.].

The closest analogue of Fig�plants is a method of determining whether to conduct immunological examinations in patients with chronic infectious and inflammatory diseases (HYVS) of different localization, namely that perform clinical analysis of blood and determine the leukocyte index - the index of the ratio of neutrophils and lymphocytes (ICNL) and lymphogranulomatosis index (ILG). Carry out bacteriological examination, consisting in the identification of microorganisms by microscopy of leikola blood, and the detection of microbial associations, including gram-positive, gram-negative bacteria and yeast, or while a higher value ILG and reduced the value ISNL respectively compared with the reference values and the identification of any type of microorganisms by microscopy of leikola blood is considered an immunological study appropriate patients HIVS different localization [patent RU 2496108, 2013].

However, this method involves conducting research at the notoriously ill patients undergoing the treatment. Often, it is necessary a medical examination of large populations, in particular periodic medical examination of workers in the livestock sector, to decide on the feasibility of immunological examination.

The object of the invention is to provide a method of determining whether to conduct immunological examination that meets Tr�requirements of accessibility and reproducibility with a high degree of validity and reliability of the results.

The technical result when using the invention the expansion of the scope of application of the method due to the possibility of using mass periodic inspections.

The proposed method of determining the feasibility of immunological examination of workers in the livestock complex in the following way:

1. Determine the total number of microorganisms in 1 m3air in the working area.

Set the Cup with a dense nutrient medium MPA (mastopathy agar) in the sampler holder-impactor air microbiological flora-100".

Press the button marked select the desired volume of 250 l air, above the button will light red indicator light.

Produce a sample of air aspiration method, by pressing the [NETWORK] is located on the left side of the probe. After selection of a given volume of air the indicator light goes out.

The Cup of selected test mesopatamia agar is placed in a thermostat and incubated at 37°C for 24-48 hours.

Count the number of colonies on the plate, and multiply this number by 4. Get the value of the total microbial number (TBC). The results are expressed in colony forming units per 1 m3(CFU/m3).

2. Conduct medical MSDS�Tr working population (according to the Ministry of health and social development of the Russian Federation No. n April 12, 2011 "On approval of lists of harmful and (or) dangerous production factors and works at which performance preliminary and periodic medical examinations (surveys), and the Procedure for carrying out obligatory preliminary and periodic medical examinations of workers employed in heavy work and work with harmful and (or) dangerous working conditions"), reveal the presence of chronic infectious and inflammatory diseases.

3. Examine the indicators of the General analysis of capillary blood by calculating percentages of the cellular composition of peripheral blood. Calculate the index of sensitization [Soldatov A. A., Sobolev, A.V., Berenson M. V., M. Y. Kuts, Shcherbakov B. V. Bodrov, I. V., Shevtsova L. index of leukocytes in the evaluation of allergic process // Klin. lab. diagnosis. - 1997. - No. 11. 35-36] and the index of immunoreactivity (EMI) by D. O. Ivanov [Ivanov D. O. Clinical laboratory variants of the course of sepsis in newborns: author. dis. doctor. honey. Sciences. - SPb., 2002. - 62 p.].

When TBC value less than 500 CFU/m3, no HEVS, the value of IA is less 1,08 CONV. units, the value of R & d 13 cond. units considered it an immunological study. When TBC value 500-2500 CFU/m3, identifying one HEVS, the value of IA 1,08-1,3 CONV. units, the value of R & d is 13.1-15.7-CONV. units considered appropriate for an immunological study tests first level [Khai�s P. M., Pinegin B. V., Imamov H. I. Ecological immunology. - M.: Publishing house of VNIRO, 1995. - 219 p.]. When TBC value more than 2,500 CFU/m3, the presence of at least two HIUS, the value of IA 1,4-1,5 usl. units, the value of R & d 15,8-18,3 CONV. units considered appropriate for an immunological study tests the second level (table).

In the available scientific and medical and patent literature is not found identical with the method for determining the feasibility of immunological examination of workers in the livestock complex, working in conditions of high bacterial contamination of the air of the working area, which allows to make a conclusion about the novelty of the proposed technical solutions.

The research of the authors was first established and it is proved that the proposed method of determining the feasibility of immunological examination is a reliable criterion for determining the groups for further in-depth immunological examination for the timely prevention and timely conduct of immunomodulation. Thus, the claimed invention meets the criterion of "inventive step".

The proposed method is easily reproducible in terms of periodic medical examinations of large groups of workers in the livestock complex, working in conditions of microbial OSCE�tenneti the air of working zone, and when using it is achieved the specified technical result. Thus, the claimed invention meets the criterion of "industrial applicability".

A clinical examination 500 employees agriculture, working at JSC "Turbulence broiler and sue the Racetrack "Akbuzat.

According to the results of medical examination formed three groups:

1. Healthy.

2. Persons with chronic diseases, without exacerbation.

3. Individuals with the presence of clinical symptoms of chronic diseases in the acute stage.

All surveyed held an expanded immunological tests. In assessing the various elements of the immune system used immunological tests I-II levels. Immunophenotyping of lymphocytes was performed by indirect immunofluorescence using monoclonal antibody subpopulations (CD3+, CD4+, CD8+, CD 16+, CD 19+, CD95, HLA DR).

The definition of the content of IgA, IgM, IgG, IgE, secretory IgA (slgA), carried out by enzyme immunoassay (ELISA) using test systems appropriate classes (JSC "Vector-best",) measurements made on an automatic analyzer "Lazurit", USA.

The degree of balance in the system of immune protection are investigated on the basis of correlation analysis of indicators of immune status.

Further, analyzed proposals�nye the immune system depending on group membership.

It was revealed that the persons of the 1st group showed adequate reaction of the body, particularly the immune system of the factors of production. Studied immune parameters had no significant differences with the control group.

In individuals of the 2nd group observed the failure of adaptation, particularly of the immune system: impact on factors of production that can be realized in the development of chronic inflammatory reaction from the shock body. Statistical analysis of the results indicates a significant (p<0,05) increase of the number of persons with reduced indicators of cellular immunity compared with the control group. In individuals of the 3rd group is the development of clinical symptoms of immune deficiency due changes in both cellular and humoral (p<0,05) relative to control. At this stage it is necessary to conduct a deeper examination of the immune status in a hospital on the subject of a decision of a question on necessity of immunocorrection.

The invention is illustrated by the following examples.

Example 1.

G R. V., 1978, the employees of incubation, TBC in the working area of 400 CFU/m3, clinical examination of chronic infectious and inflammatory diseases have not been identified, estimated �ndexi allergies and the immune response - in the normal range. Conducted immunological study revealed no significantly significant changes in indicators of immune status, which indicates the inappropriateness of his conduct. Re-examination by the 1 year did not reveal any pathological processes in the organism of patients.

Example 2.

A. the employees of poultry processing, meat balls carcasses, and TBC in the working area of 2400 CFU/m3, clinical examination revealed chronic bronchitis beyond the acute stage, IA - 1,0, R & d and 13.2. Appointed immunological examination tests of the first level. Conducted immunological study revealed a statistically significant decrease in cellular immunity - phagocytic activity of leukocytes and NBT test (stimulated variant). Recommended restorative therapy.

Example 3.

Z. shop employee parent stock, attendant, TBC in the air of working zones 7000 CFU/m3, clinical examination revealed chronic allergic rhinitis and chronic tonsillitis beyond the acute stage, IA - 1,5, R & d is 18.0. Appointed immunological examination tests of the second level.

Conducted immunological study revealed a statistically significant decrease in both cellular and humoral immunity. Conclusion: you�been immune insufficiency, required additional tests to conduct immunotherapy.

Method of determining the feasibility of immunological examination of workers in the livestock complex, including the identification of chronic infectious and inflammatory diseases (HEVS), General blood test, bacteriological examination, characterized in that the counting allergization index (IA) and the index of immunoreactivity (MIT), determine the total number of microorganisms in 1 m3the air of the working area by counting the total microbial number (TBC), TBC value less than 500 CFU/m3, no HEVS, the value of IA is less 1,08 CONV. units, the value of R & d 13 cond. units consider inappropriate conduct immunological studies; TBC 500-2500 CFU/m3, identifying one HEVS, the value of IA 1,08-1,3 CONV. units, the value of R & d is 13.1-15.7-CONV. units considered appropriate for an immunological study tests the first level; when the value of TBC more than 2500 CFU/m, the presence of at least two HIUS, the value of IA 1,4-1,5 usl. units, the value of R & d 15,8-18,3 CONV. units considered appropriate for an immunological study tests the second level.



 

Same patents:

FIELD: medicine.

SUBSTANCE: DNA from peripheral venous blood is extracted. An analysis of a combination of genetic versions of polymorphous markers of genes of cytokines of the gene regulator of the activity of normal expression and secretion of T-cells (-403 G/A RANTES), macrophage protein -1β (+1931 A/T MIP 1β), factor of stromal cells (-801 G/A SDF1), interleukin -1 (-511 C/T IL-1B), monocyte chemoattractant protein -1 (C/G MCP-1), interleukin -4 (-590 C/T IL-4) is performed. An increased risk of development of a combination of uterine myoma with endometriosis and hyperplastic processes of the endometrium is predicted if the combination of alleles 403 A RANTES, G MCP-1,+1931 A MIP 1β, -590 C IL-4 or the combination of alleles -403 A RANTES,+1931 A MIP 1β, -801 G SDF1, -511 C IL-1B is identified.

EFFECT: application of the claimed method makes it possible to detect a group of patients with a risk of developing a combination of proliferative reproductive system diseases, which makes it possible to prescribe an adequate therapy to prevent further progressing of the diseases.

3 dwg, 2 ex

FIELD: medicine.

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EFFECT: invention makes it possible to realise early prediction of increase of arterial pressure level in women in late pregnancy, will make it possible to form of women at the stage of pregravidal preparation and at early terms of pregnancy groups of high risk of developing hypertension in late pregnancy, as well as realise required therapeutic-preventive measures aimed at prevention of development of said pregnancy complication in due time.

2 dwg, 2 tbl, 1 ex

FIELD: medicine.

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1 tbl, 2 ex

FIELD: medicine.

SUBSTANCE: invention relates to field of medicine, in particular hepatology and infectious diseases, and can be used for determination of stage of fibrous process in monitoring of patients with chronic hepatitis C. To realise method levels of blood serum cytokins are determined in patients with chronic hepatitis C with diagnosed by means of biopsy or other non-invasive method stage of fibrous process 2 times a year, with further calculation of cytokine profile integral index (CPII) on their basis, at initial stage F0 growth of CPII higher than -8 testifies to debut of fibrous changes in liver (transition to stage F1), at initial stage F1, drop of CPII below -10 testifies to transition to stage F2, at initial stage F2 growth of CPII higher than -3 testifies to transition of fibrosis to stage F3, at initial stage F3 drop of CPII below -3 testifies to development of cirrhosis.

EFFECT: determination of stage of fibrous process in monitoring of patients with chronic hepatitis C.

3 ex, 1 tbl, 5 dwg

FIELD: medicine.

SUBSTANCE: invention relates to medicine, namely to a method of predicting spontaneous onset of pregnancy in women with I and II stage of external genital endometriosis. The essence of the invention consists in the fact that before treatment in peripheral blood of women with infertility, associated with I and II stage of external genital endometriosis determined is a relative quantity of IL- IL-1β + monocytes, and if the value of the said index is 50.0% and higher in the monocytic gate the spontaneous onset of pregnancy within a year after carrying out the surgical treatment of endometriosis is predicted.

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1 tbl, 3 ex

FIELD: medicine.

SUBSTANCE: peripheral blood serum interleukin-6 is measured by an immunoassay; if the measured level is more than 5 pg/ml, a biocontrol session of the heart rate variability is predicted to be effective and is expected to represent the total spectrum power gain of the heart rate variability combined with a drop in a regulatory system strain index in relation to references.

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1 tbl

FIELD: medicine.

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5 cl, 10 dwg, 2 tbl, 9 ex

FIELD: medicine.

SUBSTANCE: group of inventions relates to medicine, namely to immunology, and can be used for the ex-vivo determination of the cancer treatment efficiency. For this purpose, a level of activated T-lymphocytes (CD3+ CD69+) in an organism is measured after the introduction to a subject of one or more doses of an immunogenic composition. The level of the activated T-lymphocytes (CD3+ CD69+) higher than approximately 10.4% testifies to the fact that the subject is such that demonstrates successful clinical outcome for treatment; that is an increase of the survival rate.

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7 cl, 1 ex, 2 dwg

FIELD: medicine.

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2 dwg, 2 ex

FIELD: medicine.

SUBSTANCE: invention aims at treating drug-induced dry eye syndrome (DI-DES). Treating DI-DES implies taking the past medical history, measuring tear production and eye xerosis values reduced and increased respectively in relation to the norm. Unpreserved ocular hypotensive medications are prescribed in the patient. Unpreserved artificial tears are also applied. The lachrymal fluid is analysed by a multicytokine technique. If the analysis shows increased concentrations of proinflammatory cytokines - interleukin-6, interleukin-8, interleukin-12, Th-1 - interleukin-2, interferon-gamma, and Th-2 - interleukin-4, by min 30% in relation to the patient's age norm, a chronic immune ocular inflammation is detected. That requires transpalpebral Blepharogel-1 phonophoresis and 1% hydrocortisone ointment phonophoresis on the sub-mastoidal region from both sides; the therapeutic course is 8-10 daily procedures.

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1 tbl, 2 ex

FIELD: medicine.

SUBSTANCE: senior guinea pigs are exposed to optical light generated by light emitting diodes or fluorescent tubes at a colour temperature of 4,500 K within the wavelength region of 360-460 nm for various time distances. The exposure is assessed by functional activity indices of neutrophilic granulocytes and a mononuclear cell count.

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3 tbl

FIELD: medicine.

SUBSTANCE: invention relates to laboratory diagnostics, namely to a method for the preservation of protein of urine, sampled for the diagnostics of infectious haemorrhagic fever with renal syndrome (HFRS) by PCR method. The essence of the method consists in the following: urine is sampled from a patient in an acute period of a disease, the protein is separated, precipitated by a method of salting-out with ammonium sulphate with its further drying and preservation at a temperature of 20-24°C. Diagnostics by means of PCR method is carried out after salt removal by a method of dialysis with flowing water for 24-48 hours of a mass, obtained by mixing 3-5 g of the dry protein and 8-12 ml of distilled water.

EFFECT: application of the claimed method makes it possible to realise HFRS diagnostics after many years with the application of urine protein, preserved in a dry form.

1 dwg, 1 ex

FIELD: medicine.

SUBSTANCE: method involves measuring blood leukocytes, blood leukocyte shift index (BLSI), neutrophil/lymphocyte ratio (NLR), leukocytal intoxication index (LII). If observing BLSI >4, NLR >5, LII >4 and detecting TNFα G-308A gene mutation, an increasing intensity of a systemic inflammatory reaction is predicted in the given category of patients.

EFFECT: using the given method enables predicting a degree of the intensity of the systemic inflammatory reaction in geriatric patients suffering from acute myocardial infarction, performing primary prevention in the patients with TNFα G-308A polymorphism detected and the positive medical history of the ischemic heart disease, as well as the secondary prevention of the complicated acute myocardial infarction.

3 ex

FIELD: medicine.

SUBSTANCE: method involves analysing the newborn's clinical and functional health indicators, namely the ventilation parameters: fractional oxygen concentration in the inhaled mixture, mean airway pressure, partial oxygen pressure in the arterial postductal blood, and calculating an oxygenation index. If the oxygenation index is 3.5 or less, non-invasive pulmonary ventilation is considered to be effective, whereas the oxygenation index of more than 3.5 shows therapeutic ineffectiveness.

EFFECT: method enables predicting a success rate of various techniques for non-invasive pulmonary ventilation in premature newborns with a very low and extremely low birth body weight diagnosed with respiratory distress syndrome.

2 ex

FIELD: medicine.

SUBSTANCE: method involves analysing the newborn's clinical and functional health indicators, namely the ventilation parameters: fractional oxygen concentration in the inhaled mixture, mean airway pressure, partial oxygen pressure in the arterial postductal blood, and calculating an oxygenation index. If the oxygenation index is 3.5 or less, non-invasive pulmonary ventilation is considered to be effective, whereas the oxygenation index of more than 3.5 shows therapeutic ineffectiveness.

EFFECT: method enables predicting a success rate of various techniques for non-invasive pulmonary ventilation in premature newborns with a very low and extremely low birth body weight diagnosed with respiratory distress syndrome.

2 ex

FIELD: medicine.

SUBSTANCE: patient's pre-operative health is assessed with taking into account the presence/absence of pulmonary and renal diseases, erythrocyte Rh C-antigen, hyperinosemia of more than 4 g/l and erythrocyte count of 4.81×1012/l and less; a haemolytic perfusion risk (HPR) is calculated by formula HPR=(6.38×Xpulm+8.93×Xhyp+3.32×XEr)-(4.10×Xren+2.95×XC), wherein the numerical values 6.38; 8.93; 3.32; 4.10; 2.95 are coefficients; Xpulm is the presence (1)/absence (0) of pulmonary diseases; Xhyp is the presence (1)/absence (0) of pre-operative hyperinosemia; XEr is the presence (1)/absence (0) of the pre-operative erythrocyte count 4.81×1012/l and less; Xren is the presence (1)/absence (0) of renal diseases; XC is the presence (1)/absence (0) of erythrocyte Rh C-antigen; if HPR>6.1, pronounced erythrocyte lysis is predicted; HPR<6.1 shows moderate erythrocyte lysis.

EFFECT: method enables increasing accuracy, information value and accessibility of the erythrocyte lysis prediction.

2 ex, 1 dwg, 3 tbl

FIELD: biotechnologies.

SUBSTANCE: invention can be used for determination of atherogenicity of immune complexes containing multiply modified low density lipoproteins (IC-MMLDLP). For this purpose basic calcium superphosphate of IC-MMLDLP is prepared from human blood serum by treatment with the buffer containing 10% solution of polyethyleneglycol with a molecular weight 3350 (PEG-3350), in the ratio 1:2.5, then it is incubate within 10 minutes at a room temperature. IC-MMLDLP aggregates are pelleted, dissolved in the buffer without PEG-3350, analysed for the content of cholesterol in immune complexes (ChIK) and the level of guinea pig complement binding (EBC) by precipitated immune complexes. IC-MMLDLP atherogenicity is calculated as SSK to HIK ratio. If the value is below 24 units a high blood atherogenicity because of the reduced complement activating IgG function in IC-MMLDLP is stated.

EFFECT: method allows to assess IC-MMLDLP atherogenicity, diagnose atherosclerosis at a preclinical stage, and also to predict both the course of atherosclerotic process at individuals, and efficiency of the conducted therapy.

1 tbl

FIELD: medicine.

SUBSTANCE: invention can be used for the instant assessment of atherogenicity of the immune complexes (IC) of human blood serum. Precipitated ICs from human blood serum are prepared by treating with a buffer containing 10% polyethylene glycol of molecular weight 3,350 (PEG-3350) in ratio 1:3.5, incubating for 10 min at room temperature. Aggregated ICs are deposited by centrifuging and dissolved in PEG-3350 free buffer; the immune complex cholesterol (ICC) is measured, and a guinea pig's complement fixation (CF) by the precipitated immune complexes is determined. The IC atherogenicity is calculated as CF/ICC relation, and if the derived value is less than 4 units, the high blood atherogenicity is stated.

EFFECT: higher assessment accuracy.

1 tbl

FIELD: medicine.

SUBSTANCE: measured are: erythrocyte sedimentation rate, total bilirubin, prostacyclin, erythrocyte aggregation, blood viscosity in microcirculatory vessels, carbamide, adhesion of thrombocytes, plasminogen and nitrites. The measured data are used to assess the erythrocyte membrane resistance by coefficient (Kms). If Kms is -0.1 or less, the erythrocyte membrane appears to be ischemic resistant, while Kms of 0.0 or more shows the ischemic resistant membrane.

EFFECT: using declared method enables fast, accurate and objective assessment of the individual ischemic resistance of erythrocytes.

2 ex

FIELD: medicine.

SUBSTANCE: invention represents a method for predicting rhinosinusitis polyposa in the patients with bronchial asthma, which is implemented by analysing the patient's blood to measure endotoxemia parameters as follows: leukocytes, average weight molecules, creatinine, carbamide and erythrocyte sedimentation rate; the prediction is made by a discriminant equation: D=6.900×leukocytes(×10^9/l)+2.640×erythrocyte sedimentation rate (mm/h)+17.819×average weight molecules (absorbance units)+1.127×creatinine (mcmole/l)+24.801×carbamide(mcmole/l), wherein D is a discriminant function having a threshold value of -223.12; if D is equal or more than the limit, rhinosinusitis polyposa is predicted in the patients with bronchial asthma, and if D is less than the limit, the patients with bronchial asthma are predicted to develop rhinosinusitis polyposa.

EFFECT: provided high-accuracy prediction of rhinosinusitis polyposa in the patients with bronchial asthma.

2 ex

FIELD: medicine.

SUBSTANCE: invention relates to laboratory methods for blood analysis. Plasma is dropped in copper sulfate solution with density 1.023 g/cm3, not above, and time for drop falling on bottom of graduated cylinder with column height 243 mm is measured. The blood plasma density value is calculated by the formula:

wherein is the unknown blood plasma density (g/cm3); is copper sulfate solution density measured by areometer (g/cm3); t is average falling time of plasma drop in the copper sulfate solution (as seconds); 0.260130126 and 0.00290695 are correction coefficients. Temperature of plasma and copper sulfate solution is 20oC. Method is simple and suitable and allows carrying out analysis of small volumes of blood plasma and to reduce analysis time.

EFFECT: improved assay method.

2 ex

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