Method for preparing sterile nanoemulsion of perfluororganic compounds

FIELD: medicine.

SUBSTANCE: invention represents a method for preparing a sterile nanoemulsion of perfluororganic compounds (PFOC) involving: adding a PFOC mixture to an aqueous solution of a stabilising agent; homogenising the PFOC mixture with the aqueous solution of the stabilising agent to produce a PFOC pre-emulsion; mixing the PFOC pre-emulsion with a salt-water solution to produce the PFOC nanoemulsion; keeping the PFOC nanoemulsion at a temperature from 2 to 10°C for at least 18 hours. The method can be also implemented as follows: pre-filling a circulation loop of a PFOC nanoemulsion generating plant with the aqueous solution of the stabilising agent; adding the PFOC mixture to the aqueous solution of the stabilising agent; homogenising the PFOC mixture with the aqueous solution of the stabilising agent to produce the PFOC pre-emulsion; mixing the PFOC pre-emulsion with the salt-water solution to produce the PFOC nanoemulsion.

EFFECT: higher stability of the PFOC emulsion and prolonging the storage life.

30 cl, 7 ex, 5 tbl, 1 dwg

 

The present invention relates to chemical-pharmaceutical industry and represents a method of producing a stable, sterile nano-emulsion performancesin compounds (PFOS) for use in human and veterinary medicine as gas substitutes of donor blood and multifunctional perfluorocarbon preparations for partial maintenance of the transport gas, the volume of circulating blood and some other physiological parameters, as well as in medicines for the treatment of systemic and local disorders of blood flow, hypoxic and ischemic conditions, improving the mass transfer of gases and metabolites between blood and tissues, maintain the function of isolated organs and tissues, reduce inflammation phenomena, conduct of infusion-transfusion therapy in shock and blood loss.

The level of technology

Perfluorocarbon emulsions are getparentname emulsion based performancesin compounds used in the biomedical field as multifunctional medicines. From the point of view of colloid chemistry perfluorocarbon getparentname emulsion is direct, concentrated, high - and freely-dispersed, heterogeneous, thermodynamically unstable lifornia colloidal systems with excess free surface�a combined energy and a huge area of gas exchange (sorption active phase boundary surface), in which the disperse phase is insoluble ultrafine chemically inert perfluorocarbon particles covered with the adsorption-solvate layer of surfactant and retains a certain time at low temperatures, aggregate stability and uniform distribution of the particles of the dispersed phase by volume dispersion of the structured environment.

High energy saturation perfluorocarbon particles and their ultradispersed leads to special properties and puts them in a special transitional region (10-8m) near colloidal (10-9m) and molecular (10-10m) state of matter. This is a special state perfluorocarbon particulate nanosystems is manifested in their high biological activity, reactivity, physical interaction with any substances and gases.

The main parameters of the emulsion PFOS, which determine their quality and potential use are:

1) the average particle diameter of emulsion PFOS;

2) distribution of emulsion particles by diameter;

3) the half-life of PFOS from the body;

4) the toxicity of the emulsion or the LD50;

5) the oxygen capacity of the emulsion;

6) the magnitude of the vapour pressure of PFOS;

7) the sterility of the emulsion;

8) the possibility of long-term storage of the emulsion;

9) toxicity stabilizing DOB�Ki;

10) reactogenicity of the emulsion.

The comparison of these indicators allows to estimate the resulting emulsion PFOS and to determine the advantage of one emulsion PFOS in front of the other.

The average particle diameter of emulsions depends on the properties of PFOS, and on the ability of stabilizing additives (emulsifier) reduce the surface tension at the boundary of PFOS water. The smaller the value of surface tension, the easier the process of emulsification and the smaller the average diameter of the particles in the resulting emulsion.

Emulsion PFOS with varying stabilizing additive should have a long shelf life and storage conditions should be simple. The rate of storage of the emulsion PFOS is the change (increase) the average diameter of the particles of the emulsion during storage.

The main tasks upon receipt of the gas-emulsion PFOS are:

1) the reduction in the average particle diameter of the emulsion PFOS and narrow the distribution of the emulsion particles by diameter;

2) reliable sterilization of the emulsion PFOS;

3) lack of toxicity of the emulsion PFOS;

4) the increase of terms and simplification of the conditions of storage of the emulsion PFOS without a significant change in the average particle diameter and the broadening of the distribution of particle diameters.

It is known that the quality of perfluorocarbon emulsions is determined by the physico-chemical properties select�nnyh PFOS physico-chemical properties and the nature of stabilizing additives and technology of emulsion.

PFOS is insoluble in water and are poor solvents for various water-soluble biologically active substances, therefore, the application as kislorodprovodyashchikh their environments is dispersed in an aqueous solution of stabilizing additives to the formation of finely dispersed emulsions.

The task of stabilizing additives consists in the formation of the adsorption layer around the particles perfluorocarbons, physico-chemical properties and the nature of stabilizing additives determine the stability of the dispersed system and its reactogenicity. The key parameters here are the bond strength of the surfactant with the "core" of the particles of the emulsion, the nature and density of the surfactant molecules on the surface of the particle.

Various methods for the obtaining of emulsions of PFOS. One of the technologies based on the method of grinding the oily phase with the aid of ultrasound. However, the energy and power of ultrasonic influence so great, that along with the dispersion breaks the link C-F. as a result, In the aqueous phase of the emulsion appear highly toxic ion concentration F-and of their excess content of the emulsion must be cleaned by passing through ion exchange resin. In addition, Taka� emulsion has an extremely broad size dispersion.

Mechanical dispersion by shaking or mixing, you will get only coarse emulsion PFOS with unacceptable for medical use size - 1 mm. To obtain a fine emulsion using the method of extrusion of the substance of the dispersed phase through a thin hole in a dispersion medium under high pressure (extrusion method), which leads to the rupture of a moving liquid jet into droplets. Dispersion is caused by pressure gradient forces and hydraulic friction. Usually the production of emulsions PFOS was performed at the high-pressure homogenizers, which allows to obtain a sufficiently thin calibrated emulsion.

Homogenization is achieved by passing the mixture of emulsifier and perfluorocarbon through the small holes with a cross section of 10-6-10-8m2under pressure from 10.1 to of 101.3 MPa. To obtain emulsions previously used single-loop method, where the perfluorocarbon from a separate container drip fed into the container of emulsifier (poloxamers), then the mixture was fed into a disintegrator and back from it in a container of PFOS and emulsifier. This cycle is repeated many times (5 to 15 cycles). However, the emulsion obtained in this way do not satisfy the requirements of particle size, as the particles remain with the size�m 400-450 nm, which increases the toxicity of the resulting emulsion.

A method of producing emulsions of PFOS for medical purposes, in which to reduce reactogenicity suggested to reduce the average size and increase the monodispersion of particles of the emulsion. This is achieved by intravenous injection of a mixture of two types of liquid PFOS in aqueous solution of a stabilizing agent that prevents macroscopic phase boundary between, increases the time and the contact surface of PFOS and stabilizing agent at the stage of obtaining predementia. Submicron emulsion is obtained in the double-circuit system of homogenization in the process 12x recirculated to the contours of the homogenizer with alternating pressure. However, the first and second loops of the homogenizer are used interchangeably, which slows down the process of homogenization, as the returns from the second circuit to the first lead to the introduction in crushed finely dispersed emulsion of large particles of predementia and even drops PFOS inevitably formed and retained on the chamber walls and pipes in the primary circuit.

In the patent of the Russian Federation 2122404 a method for producing emulsions by inkjet bandwidth of multicomponent mixtures of PFOS and the solution proxenol through a homogenizer under a pressure of 700-1000 ATM and cooled at a temperature of from 24 to 34°C with further cyclic the homogeneity of energy�ization under constant pressure 400-490 ATM and at a constant cooling. In the proposed method of producing emulsions due to jet passing the mixture of PFOS reduces the time by half of the first cycle of homogenization, and the regulation and monitoring of pressure in the homogenizer prevent the secondary process of particle agglomeration and provide gentle shaping the emulsion to a predetermined particle size of 0.03-0.05 microns.

In the patent of the Russian Federation 2206319 a method for producing the emulsion according to which before mixing the components of the mixture of liquid PFOS and an aqueous solution of a stabilizing agent is saturated with carbon dioxide, after which the heated aqueous solution of a stabilizing agent at a temperature not exceeding 75°C. Then the mixture of liquid PFOS administered multiple jets in a chilled aqueous solution of a stabilizing agent with vigorous stirring and blowing of carbon dioxide, simultaneously flowing the resulting coarse prizemoney several times through the first circuit of the homogenizer, and then chopped prizemoney is subjected to homogenization in the second circuit of the homogenizer to obtain the desired fineness while blowing carbon dioxide, and add water and salt composition.

The saturation of the blend components with carbon dioxide and heating the aqueous solution of stabilizing agent (proxenol) allows you to depyrogenation solution without passing through the sorbent, worsening ka�the ETS stabilizing agent and modifies its molecular weight distribution, and surface-active properties. Deviations from the specified temperatures degrade the properties of surfactant stabilizing agent. Bolus of liquid PFOS in aqueous solution of a stabilizing agent with vigorous stirring and simultaneous passage of the mixture through the homogenizer high pressure accelerates the process of obtaining predementia. The control the feed rate of PFOS, mixing and flow of the mixture through the homogenizer is carried out so as to prevent the formation of macroscopic phase boundary between PFOS/water, which is a necessary condition for obtaining a monodisperse emulsion.

In the patent of the Russian Federation2070033 a method for producing a perfluorocarbon emulsions for medical purposes, in which the mixture of PFOS is produced by mixing liquid components, and predementia receive, passing the mixture of PFOS using an aqueous solution of a stabilizing agent (proxenol), obtained at the same time letting prizemoney the working chamber of the homogenizer, after complete mixing is passed through the working chamber of the homogenizer with a temperature control under pressure 300-660 ATM, followed by the addition obtained in prizemoney sterile physiologically acceptable water-salt solution. The resulting nanoemulsion PF�With frozen.

The disadvantages of the above-described method of producing nano-emulsions PFOS are the complexity of producing a mixture of PFOS related to the fact that weigh the liquid components of different densities to obtain the desired volume of the mixture of PFOS; temperature control is carried out only working chamber of the homogenizer that does not provide the required temperature to obtain predementia with an average particle size of 30-80 nm; pressure in the working chamber of the homogenizer above 60 MPa is excessive and leads to zagliani aqueous solution poloxamer; the freezing of nano-emulsion PFOS immediately after production leads to a dramatic enlargement of the particles of the nano-emulsion PFOS, which reduces its stability, reducing the shelf life.

In the patent of the Russian Federation2393849 a method for producing the emulsion PFOS in which, prior to mixing, the mixture of PFOS and the aqueous solution poloxamer saturate with carbon dioxide, the process of preparation of predementia and subsequent homogenization is carried out in an atmosphere of carbon dioxide, in prizemoney adds a physiologically acceptable aqueous saline solution.

The disadvantages of this method of obtaining nano-emulsion PFOS are a mixture of PFOS and aqueous solution poloxamer carbon dioxide and implementation of the homogenization process in the atmosphere of carbon dioxide that the process�ski difficult to realize and appreciate the process of making nano-emulsion PFOS. The volume of production of nano-emulsion is limited by the performance of the homogenizer. Water-salt solution is added to prizemoney with higher density, which affects the mixing and increases the process. There is no stabilization nano-emulsion PFOS before freezing.

The aim of the present invention is to provide a simple, cost-effective method of producing nano-emulsions of PFOS, which allows to obtain a product with improved stability and simplify storage conditions of nano-emulsions PFOS without a significant change in the average particle diameter and the broadening of the distribution of particle diameters.

The authors present invention it was unexpectedly found that pre-filling the circulation circuit of the apparatus for producing nano-emulsion PFOS, which includes the capacity to obtain predementia PFOS, homogenizer, and piping connecting the tank and the homogenizer, the solution stabilizing additive mixture before adding PFOS reduces the average particle diameter of the emulsion and reduce the distribution of particle diameters, which, in turn, greatly increases the stability of the emulsion PFOS and as a result increases the shelf life of the product.

In addition, the authors present invention it was unexpectedly discovered that the exposure of the emulsion PFOS �ri a temperature of from 2 to 10°C for more than 18 hours before freezing the finished product also can significantly increase the stability of the emulsion PFOS.

Brief description of the invention

The present invention relates to a method of producing sterile nano-emulsion performancesin compounds (PFOS), which includes:

- add mixture to PFOS aqueous solution of a stabilizing additive;

- homogenization of the mixture of PFOS from aqueous solution of stabilizing additives with getting predementia PFOS;

- mix predementia PFOS from water-salt solution to obtain nano-emulsion PFOS;

- maintaining nano-emulsion PFOS at a temperature of from 2 to 10°C for at least 18 hours;

- optional freeze nano-emulsion PFOS.

In a preferred embodiment of the method according to the present invention is carried out pre-filling the circulation circuit of the apparatus for producing nano-emulsion PFOS aqueous solution of a stabilizing additive.

The present invention also relates to a method of obtaining sterile nano-emulsion performancesin compounds (PFOS), which includes:

- pre-filling the circulation circuit of an aqueous solution of a stabilizing additive;

- add mixture to PFOS aqueous solution of a stabilizing additive;

- homogenization of the mixture of PFOS from aqueous solution of stabilizing additives with getting predementia PFOS;

- mix predementia PFOS from water-salt solution with the floor�the increase of nano-emulsion PFOS;

- optional freeze nano-emulsion PFOS.

The circulation loop in the method of the present invention includes a container for receiving predementia PFOS, homogenizer, and piping connecting the tank and the homogenizer.

In a preferred embodiment of the method according to the present invention before processing, carry out leak test the piping system by flushing sterile a concentrated aqueous solution of a stabilizing additive in the amount of 2-4 times the working volume of the piping and the working chamber of the homogenizer at a pressure in the working chamber of the homogenizer 10-45,5 MPa, with subsequent draining of the solution.

In a preferred embodiment of the method according to the present invention homogenization of predementia PFOS carried out at a pressure in the working chamber of the homogenizer in the range from 10 to 60.8 MPa, preferably in the range of 43.1 to 45.1 MPa, carry out the temperature control of the working chamber of the homogenizer to provide process temperature in the range from 19 to 30°C, preferably from 20 to 26°C and carry out the temperature control of the circulation circuit piping to maintain the process temperature of the piping in the range of 5 to 20°C, preferably from 7 to 15°C.

In a preferred embodiment of the method according to the present invention qi�colazioni circuit contains one or more additional homogenizers, connected in parallel to the main homogenizer circulation loop.

In a preferred embodiment of the invention, the homogenizer has more than one working chamber, connected in parallel.

In a preferred embodiment of the method according to the present invention, a nanoemulsion PFOS before standing bottled in consumer packaging.

In the most preferred embodiment of the method according to the present invention consumer packaging with nanoemulsion PFOS before freezing gently shaken until the homogeneity of the content.

Consumer packaging, which is used in the method according to the present invention, is a glass bottle, vials, ampoules, syringe-tubes, plastic containers, metal containers, tubes.

In a preferred embodiment of the method according to the present invention the process is carried out in aseptic conditions.

In a preferred embodiment of the method according to the present invention an aqueous solution of a stabilizing additive is a 10-30 mass. % aqueous solution, preferably 10-20 wt.% aqueous solution and as a stabilizing additive used poloxamer, such as copolymers of polyoxyethylene and polyoxypropylene, preferably poloxamer with the trade names Amoxil 268 brand "A" and Killiphor P188 (Califor P188)10-C22fatty acid�you and/or their salts or triglycerides or phospholipids of egg yolk or soybean.

In the method according to the present invention as a mixture of PFOS use a mixture of at least one quickly withdrawn PFOS selected from C8-C10PFOS, for example, performanceline (PFD) or performancebased (PFOB) and at least one slowly withdrawn PFOS selected from C11-C12PFOS, such as performapply (PPTP), (PFMRP) or pftba (PFTB).

In a preferred embodiment of the method according to the present invention a mixture of PFOS for the preparation of predementia prepared by mixing one or more liquid PFOS.

In the method according to the present invention a water-salt solution comprises NaCl and water, and optionally KCl, MgCl2, NaHCO3, NaH2PO4, Na2HPO4, glucose.

In a preferred embodiment of the method according to the present invention, the nanoemulsion contains PFOS 4,8-7,2 g/l of sodium chloride; 0.31 and 0.47 g/l of potassium chloride; 0,15-0,23 g/l of magnesium chloride (in terms of dry substance); 0,55-0,83 g/l sodium bicarbonate; 0,16-0,24 g of sodium phosphate onesemester (in terms of dry substance); 1.6 to 2.4 g/l of glucose.

In a preferred embodiment of the method according to the present invention the pH of the nano-emulsion PFOS is from 5 to 8, preferably in the range from 7.2 to 7.8, and for the adjustment of pH change, the quantitative content of sodium hydrocarb�Nate and sodium phosphate onesemester.

In a preferred embodiment of the method according to the present invention, a nanoemulsion PFOS frozen at a temperature of from -4 to -18°C, preferably at temperatures from -10 to -18°C.

In a preferred embodiment of the method according to the present invention, a nanoemulsion PFOS maintained at a temperature of from 2 to 10°C, preferably at a temperature of from 3 to 6°C, most preferably at 4°C.

In a preferred embodiment of the invention, the emulsion can withstand before freezing for at least 18 hours, preferably for 18 to 30 hours, most preferably 24 hours.

Detailed description of the invention

Fig.1 shows a preferred circuit for receiving nanamoli PFOS for carrying out the method of the present invention.

In the presented scheme the container 1 is a container for a mixture of PFOS or solution stabilizing additive, the conduit 2 connects the container 1 with the container 3 circulation circuit to produce a mixture of PFOS in solution stabilizing additive pipes 4 and 6 circulation circuit connecting capacity 3 homogenizer with 5 high-pressure circulation loop, the conduit 7 connects the circulation circuit with capacity for 8 water-salt solution to obtain the prepared nano-emulsion PFOS, the conduit 9 is designed to remove gotovo� product and/or packaging of finished nanoemulsion PFOS in consumer packaging.

The method is as follows.

For more predementia PFOS mixture of PFOS is served drip and/or bolus from the tank 1 through the pipeline 2 into the container 3 with a solution of a stabilizing additive. In a preferred embodiment of the invention before the start of the process the aqueous solution of the stabilizing additive is fed from the tank 1 into the main circulation circuit, comprising a container 3, the pipes 4 and 6 and homogenizer 5 to completely fill the loop.

A mixture of PFOS in aqueous solution stabilizing additive is pumped basically a circulation loop through pipelines 4 and 6 through the homogenizer 5 at an operating pressure in the chamber of the homogenizer 10-60,8 MPa, cooling the working chamber of the homogenizer to 19-30°C and the temperature control piping 4 and 6 circulation circuit at a temperature of 5-20°C. is Carried out 9-15 cycles of homogenization to obtain predementia PFOS. Then prizemoney PFOS pumped through conduit 7 into the container 8 with a water-salt solution, where predementia, gradually passing through the water-salt solution, specific gravity less than the specific weight of predementia uniformly mixed with water-salt solution to obtain prepared nano-emulsion PFOS. Next, the finished product through the pipeline 9 is pumped in additional capacity or in consumer packaging, where optional stand �ri a temperature of 2-10°C for at least 18 hours for stabilization of the product, then the product is not necessarily frozen at a temperature of from -4 to -18°C.

In a preferred embodiment of the invention before the beginning of the process are checking the tightness of the circulation circuit by filling an aqueous solution of a stabilizing additive in the amount of 2-4 times greater than the working volume of the piping and the working chamber of the homogenizer when the homogenizer at a pressure of 10-45,5 MPa with subsequent draining of the solution.

The nanoemulsion of PFOS present invention includes at least one quickly withdrawing PFOS and at least one slow elimination of PFOS in the amount of 50 vol.%, preferably from 5 to 30 vol.%, even more preferably from 10 to 20 vol.%, the stabilizing additive in an amount up to 10 wt.%, preferably from 0.5 to 10 mass%, more preferably from 3.5 to 4.5 mass%, salts such as NaCl, KCl, MgCl2, NaHCO3, NaH2PO4, Na2HPO4and water.

The ratio quickly withdrawing and slowly withdrawing perfluorinated compounds may range from 10:1 to 1:10.

Water-salt part of the finished emulsion PFOS may contain 82-123 mm NaCl, and optionally a 4.2-6.3 mm KCl, 1.6 to 2.4 mm MgCl2, 6,6-9,9 mm NaHCO3That 1.3-2 mm NaH2PO4. Preferably, the ratio of Na and K ions is maintained at 10:1 to 30:1.

In the finished emulsion PFOS may also include gluco�and in the amount of 8.9 and 13.3 mm (or 1.6-2.4 g/l).

The pH of the finished emulsion PFOS is from 5 to 8.0, preferably from 7.2 to 7.8.

Emulsion PFOS has an average particle size of not more than 150 nm, preferably 30-100 nm, most preferably 30-80 nm.

To obtain emulsions of PFOS of the present invention use, as a rule, simultaneously two types performancesin compounds. One of them is selected from the group of C8-C10that includes, for example, perpendicular (PFD) or perforative (PFOB), the second group With11-C12containing, for example, performapply (PPTP), performatilicious-piperidine (PFMRP) or pftba (PFTB). The connection of the first type quickly (within a month) are eliminated from the body, but do not provide sufficient stability of the emulsions, the compounds of the second type, by contrast, give emulsions of high stability, allowing you to store them without freezing, but for a long time (8 months to 2 years) is not removed from the body.

Can also be used multicomponent mixture of two PFOS, for example, PFD/PFTB, PFD/PFMSP, PFOB/PFTB, PFOB/PFMSP or three PFOS, such as PFOB/PFD/PFMP, PFOB/PFD/PFTB or four PFOS, for example, PFOB/PFD/PFMP/PTBA.

Special stabilizing additives are added to reduce the average diameter of the particles of the emulsion, increasing the Mgr�resti and stability during prolonged storage.

As stabilizing additives are preferably used nontoxic nonionic high molecular weight surfactants (nonionic surfactants), in particular poloxamer (proxenol, pluronic, kollipara, amoxol). Their number in the emulsion PFOS as inclusive as possible, and is determined only by the need for satisfactory emulsification and homogenization of PFOS. Minimizing the amount of surfactant is associated with the fact that they affect the toxicity and reactogenicity of the emulsion PFOS.

Poloxamer are a copolymer of polyethylene oxide and polypropyleneoxide of the General formula HO(C2H4O)m(C3H6O)n(C2H4O)m,H, where (m+m') ranges from a few to a few dozen; the molecular weight is from 1,000 to 20,000, share polyoxyethylenated blocks can range from 10 to 80% by mass.

In a preferred embodiment of the present invention uses poloxamer with the trade name Proxenol 268, "Amoxol 268" brand "A", Kolliphor P188 (Califor P188), Pluronic F68 (Pluronic F68), Synperonic F68 (Synperonic F68), Lutrol F68 (Lotrel F68).

As stabilizing additives can also be used With10-C22fatty acids and/or their salts or triglycerides and phospholipids of egg yolk or soybean.

In a preferred method of the present invention used�t 10-30 wt.% an aqueous solution of a stabilizing additive, most preferably 10-20 wt.% water solution.

Water-salt solution used in the method according to the present invention is an aqueous solution of salts selected from NaCl, and optionally KCl, MgCl2, NaHCO3, NaH2PO4, Na2HPO4. Water-salt solution may contain glucose.

The number of components of a water-salt solution used for mixing with predementia PFOS is calculated in such a way as to ensure in the finished emulsion PFOS following salt content: 82,1-of 123.2 mm NaCl, 4.2 to 6.3 mm KCl, 1.6 to 2.4 mm MgCl2The 6.6-9.9 mm NaHCO3That 1.3-2 mm NaH2PO4and 8.9-13.3 mm D-glucose or 4.8-7.2 g/l of sodium chloride; 0.31 and 0.47 g/l of potassium chloride; 0,15-0,23 g/l of magnesium chloride (in terms of dry substance); 0,55-0,83 g/l sodium bicarbonate; 0,16-0,24 g/l sodium phosphate onesemester (in terms of dry substance); 1.6 to 2.4 g/l of glucose.

Below the invention is illustrated by examples, which in no way are intended to limit the scope of the claimed invention.

EXAMPLES

Example 1. Obtaining nano-emulsion containing 10 vol.% PFOS.

Before the homogenization process check the tightness of the circulation circuit by filling a 13.3% aqueous solution poloxamer Amoxil 268 brand "A" in the amount of 2-4 times greater than the working volume of the pipeline and working to�measures homogenizer, when the homogenizer at a pressure of 10-45,5 MPa, followed by drain. The receptacle 3 is filled 2,52 l 13,3% aqueous solution poloxamer Amoxil 268 brand "A". A mixture of PFOS, including 0,56 l PFD and 0.28 l PFMSP, gradually (drip) with 2,52 l 13,3% aqueous solution poloxamer Amoxil 268 brand "A". The pressure in the homogenizer is maintained in the range from 43,1 to 45.1 MPa, and cooling the working chamber up to 20-26°C. the Piping of the circulation loop was incubated for creating a temperature in the range of 7-15°C. is Carried out by 13 cycles of homogenization. Get 3,36 l predementia PFOS containing 25 vol.% PFOS with an average particle size of 50-80 nm 3,36 l received predementia add to 5.04 liters of water-salt solution containing 1.7 times more dissolved salts (95,5 mm NaCl, 5.0 mm KCl, 2.2 mm MgCl2, 8.1 mm NaHCO3, 1.5 mm NaH2PO4and 10.7 mm glucose) and the salt concentration in the nano-emulsion in the ratio 1:1.5 respectively. Get 8.4 l nano-emulsion PFOS containing 10 vol.% PFOS with an average particle size of 50-80 nm. A nanoemulsion PFOS poured into sterile consumer packaging and stabilize at 4°C for 24 hours, after which it was frozen at a temperature of -15°C.

In accordance with the methodology described in Example 1 experimental series nano-emulsion PFOS 4E with an average particle size of 64 nm.

Example 2. Obtaining nano-emulsion containing 10 vol.% PFOS.

Before filling the piping system with a solution of stabilizing additives check the tightness of the circulation circuit by filling a 10% aqueous solution poloxamer Amoxil 268 brand "A" in the amount of 2-4 times the working volume of the piping and the working chamber of the homogenizer, while the homogenizer at a pressure of 10-45,5 MPa, followed by drain. The circulation circuit is filled 3,36 l of 10% aqueous solution poloxamer Amoxil 268 brand "A", and then it gradually (jet) was added a mixture of PFOS, consisting of 0,56 l PFD and 0.28 l PFMSP. The pressure in the homogenizer is maintained in the range from 43,1 to 45.1 MPa, and cooling the working chamber up to 20-26°C. the Piping of the circulation loop was incubated for creating a temperature in the range of 7-15°C. is Carried out 10 cycles of homogenization. Get 3,36 l predementia PFOS containing 25 vol.% PFOS with an average particle size of 50-80 nm 3,36 l received predementia add to 5.04 liters of water-salt solution containing 1.7 times more dissolved salts (93,7 mm NaCl, 5.1 mm KCl, 1.9 mm MgCl2, 7.5 mm NaHCO3, 1.7 mm NaH2PO4and 11.2 mm glucose) and the salt concentration in the nano-emulsion in the ratio 1:1.5 respectively. Get 8.4 l nano-emulsion PFOS containing 10 vol.% PFOS with middle�m particle size of 50-80 nm. A nanoemulsion PFOS poured into sterile consumer packaging and stabilize at 4°C for 24 hours, after which it was frozen at a temperature of -150C.

In accordance with the methodology described in Example 2 experimental a series of nano-emulsion PFOS 3E with an average particle size of 53 nm.

Example 3. Obtaining nano-emulsion containing 10 vol.% PFOS.

Before filling the piping system with a solution of stabilizing additives check the tightness of the circulation circuit by filling a 10% aqueous solution poloxamer Lutrol F68 (Lotrel F68) in the amount of 2-4 times the working volume of the piping and the working chamber of the homogenizer, while the homogenizer at a pressure of 10-45,5 MPa, followed by drain. The circulation circuit is filled 3,36 l of 10% aqueous solution poloxamer of Amoxil 268 brand "A", and then it gradually (drip) was added a mixture of PFOS, consisting of 0,56 l PFD and 0.28 l PFMSP. The pressure in the homogenizer is maintained in the range from 10 to 81 MPa, and cooling of the working chamber to 19-28°C. the Piping of the circulation loop was incubated for creating a temperature in the range of 10-20°C. is Carried out 10 cycles of homogenization. Get the 4.2 l predementia PFOS containing 20 vol.% PFOS with an average particle size of 50-80 nm 4.2 l received predementia added in 4.2 l of water-salt solution, contains 2 times more dissolved salts (100,7 mm NaCl, 5.7 mm KCl, 2.1 mm MgCl2, 7.9 mm NaHCO3, 1.6 mm NaH2PO4, and 11.0 mm glucose) and the salt concentration in nano-emulsion, i.e. in the ratio 1:1, respectively. Get 8.4 l nano-emulsion PFOS containing 10 vol.% PFOS with an average particle size of 50-80 nm. A nanoemulsion PFOS poured into consumer packaging and stabilize at 4°C for 24 hours, after which it was frozen at a temperature of -16°C.

In accordance with the methodology described in Example 3 experimental series nano-emulsion PFOS: series 3C with an average particle size of 53 nm.

Methods method of producing nano-emulsion PFOS Examples 1, 2 and 3 represent the standard technology production, supplemented by stage of storage, the nano-emulsion before freezing in Example 1 or supplemented preliminary stage of filling the circulation circuit of an aqueous solution of a stabilizing additive in Example 2 or supplemented as the stage of storage, the nano-emulsion before freezing and stage pre-filling the circulation circuit of an aqueous solution of a stabilizing additive in Example 3.

Example 4 (control). Obtaining nano-emulsion containing 10 vol.% PFOS.

Before the homogenization process to check the integrity of circuit �UTEM fill a 13.3% aqueous solution poloxamer Amoxil 268 brand "A" in the amount 2-4 times greater than the working volume of the piping and the working chamber of the homogenizer, while the homogenizer at a pressure of 10-45,5 MPa, followed by drain. A mixture of PFOS, including 0,56 l PFD and 0.28 l PFMSP gradually (inkjet) with 2,52 l 13,3% aqueous solution poloxamer Amoxil 268 brand "A". The pressure in the homogenizer is maintained in the range from 10 to 81 MPa, and cooling of the working chamber to 19-28°C. the Piping of the circulation loop was incubated for creating a temperature in the range of 10-20°C. is Carried out by 13 cycles of homogenization. Get the 4.2 l predementia PFOS containing 20 vol.% PFOS with an average particle size of 50-80 nm 4.2 l received predementia added in 4.2 l of water-salt solution containing 2 times more dissolved salts (102,3 mm NaCl, 5.6 mm KCl, 2.0 mm MgCl2, 8.3 mm NaHCO3, 1.8 mm NaH2PO4and 11.2 mm glucose) and the salt concentration in nano-emulsion, i.e. in the ratio 1:1, respectively, Get 8.4 l nano-emulsion PFOS containing 10 vol.% PFOS with an average particle size of 50-80 nm. A nanoemulsion PFOS frozen at a temperature of -17°C.

According to the method described in Example 4, receive a control series of nano-emulsion PFOS 1K with an average particle size of 58 nm.

The method of method of producing nano-emulsion PFOS Example 4 is a standard production technology (control).

�reamer 5. Evaluation of the influence of aging of nano-emulsion PFOS or pre-fill the piping system with a solution of stabilizing additives on the stability of the product.

The stability of nano-emulsion PFOS determined to change the size of the parts during storage. Normal average size of the particles size is in the range from 30 to 150 nm.

The nanoemulsion obtained in Examples 1 (series 4E), 2 (series 3E) and 4 (series 1), thawed and determine average particle size when stored at 4°C and -18°C.

The average particle size of the emulsions and the distribution of particle diameter measured with an electron microscope.

The data obtained are presented in Tables 1 and 2.

Table 1
The stability of nano-emulsion PFOS during storage at 4°C
SeriesThe average particle size of the emulsion PFOS (nm)
0 weeks.1 week.2 weeks.3 weeks.4 weeks.5 weeks.6 weeks.7 weeks.8 weeks.
1K 5888117149-----
3E537396120147----
4E64758698114130151--

Table 2
The stability of nano-emulsion PFOS during storage at -18°C
SeriesThe average particle size of the emulsion PFOS (nm)
0 mo.6 months.12 months.18 months.24 months.30 m�S. Of 36 months.42 months.48 months.54 months.60 months.
1K5879105129150------
3E536886106124149-----
4E6471798898110125140153--

Presents measured average particle size of the emulsion PFOS show that the DDH�maintenance nano-emulsion PFOS before freezing at 4°C for 24 hours or prefilling circulation circuit solution stabilizing additives before mixing the components significantly increases the shelf life of the product compared to the control:

- shelf life nano-emulsion PFOS in Example 1 at a temperature of 4°C is 6 weeks, which is 3 weeks longer shelf life control series of Example 4 (table 1);

- shelf life nano-emulsion PFOS in Example 2 at a temperature of 4°C is 4 weeks on 1 week is more retention of control series of Example 4 (table 1);

- shelf life nano-emulsion PFOS in Example 1 at a temperature of -18°C is 48 months, which is 24 months longer than the shelf life of control series of Example 4 (table 2);

- shelf life nano-emulsion PFOS in Example 2 at a temperature of -18°C is 30 months, which is 6 more months of shelf life control series of Example 4 (table 2).

Example 6. Evaluation of the effect of pre-filling the circulation circuit solution stabilizing additives and curing nano-emulsion PFOS on the stability of the product.

The stability of nano-emulsion PFOS determined to change the size of the parts during storage. Normal average size of the particles size is in the range from 30 to 150 nm.

The nanoemulsion obtained in Examples 3 (series 3C) and 4 (series 1), thawed and determine average particle size when stored at 4°C and at -18°C.

The average particle size of the emulsions and the distribution�for the same particle diameter measured with an electron microscope.

The data obtained are presented in Tables 3 and 4.

Table 3
The stability of nano-emulsion PFOS during storage at 4°C
SeriesThe average particle size of the emulsion PFOS (nm)
0 weeks.1 week.2 weeks.3 weeks.4 weeks.5 weeks.6 weeks.7 weeks.8 weeks.
1K5888117149-----
3C5360688088101113127149

Table 4
The stability of nano-emulsion PFOS during storage at -18°C
SeriesThe average particle size of the emulsion PFOS (nm), interval 6 months
06121824303642485460
1K5879105129150------
3C5353535454535554555657

Data analysis of measurements of the average particle size of nanomoles�and PFOS shows that filling the circulation circuit solution stabilizing additives and aging of nano-emulsion PFOS before freezing at 4°C for 24 hours significantly increases the shelf life of the product compared to the control:

- shelf life at 4°With nano-emulsion of Example 3 is 8 weeks, 5 weeks more control (table 3);

- shelf life nano-emulsion of Example 3 at a temperature of -18°C is 60 months 36 months more control (table 4).

Moreover, the stability of nano-emulsion PFOS for use as fill the piping system with a solution of stabilizing additives and storage, the nano-emulsion PFOS before freezing at 4°C for 24 hours significantly increases the shelf life of the product not only in comparison with the control product, but also in comparison with the product obtained using only fill the piping system with a solution of stabilizing additives or stage of storage, the nano-emulsion.

The experimental data presented above clearly prove that the use in the method of producing nano-emulsions PFOS extra stage of pre-filling the circulation circuit of an aqueous solution of stabilizing additives and/or storage, the nano-emulsion PFOS for those�ture 2-10°C for more than 18 hours can significantly increase the emulsion stability and shelf life.

Example 7. Evaluation of the effect of pre-filling the circulation circuit solution of stabilizing additives on the distribution of particle diameters.

The average particle size of the emulsions and the distribution of particle diameters prepared by direct measurement of nano-emulsion particles using electron microscopy. For sample preparation, method used negative contrast monolayer of nano-emulsion particles deposited on a collodion film, uranyl nitrate. Images were taken at an accelerating voltage of 80 kV in an electron microscope JEM 100B (JEOL, Japan).

The data obtained are presented in Table 5.

Table 5
SeriesThe average particle size, nmThe number of homogenization cyclesThe distribution of the diameters of the nano-emulsion particles in the range from 0 to 210 nm in %
0<d<3030<d<6060<d<9090<d<120120<d<150150<d<180180<d<210210<d<240
4E 64131,249,240,54,72,91,10,30,1
3E53102,251,942,53,30,1000

The data presented clearly show that the use of pre-filling the circulation circuit solution stabilizing additives to obtain a nano-emulsion with an average particle size in the range of 30-80 nm is required for three cycles of homogenization less, which significantly reduces the production time. Also, the nanoemulsion PFOS series 4E, obtained without pre-filling the circulation circuit solution stabilizing additive has a desired average particle size of 64 nm, but unlike nano-emulsion PFOS series 3E contains particles larger than 200 nm, which increases the number reactogenic reactions when using it.

1. Method of producing article�sterile nano-emulsion performancesin compounds (PFOS), including:
- add mixture to PFOS aqueous solution of a stabilizing additive;
- homogenization of the mixture of PFOS from aqueous solution of stabilizing additives with getting predementia PFOS;
- mix predementia PFOS from water-salt solution to obtain nano-emulsion PFOS;
- maintaining nano-emulsion PFOS at a temperature of from 2 to 10°C for at least 18 hours.

2. A method according to claim 1, further comprising pre-populating the circulation circuit of an aqueous solution of a stabilizing additive.

3. A method according to claim 1 or 2, wherein the circulation circuit includes a tank for receiving a mixture of PFOS with an aqueous solution of a stabilizing additive, a homogenizer and the piping connecting the tank and the homogenizer.

4. A method according to claim 1 or 2, which is carried out in aseptic conditions.

5. A method according to claim 1 or 2, wherein, before starting the process, carry out leak test the piping system by flushing sterile aqueous solution of a stabilizing additive in the amount of 2-4 times the working volume of the piping and the working chamber of the homogenizer at a pressure in the working chamber of the homogenizer 10-45,5 MPa, with subsequent draining of the aqueous solution.

6. A method according to claim 1 or 2, wherein the homogenization of predementia PFOS carried out at a pressure in the working chamber of the homogenizer in range�not from 10 to 60.8 MPa, preferably in the range of 43.1 to 45.1 MPa.

7. A method according to claim 1 or 2, in which the temperature control of the working chamber of the homogenizer to provide process temperature in the range from 19 to 30°C, preferably from 20 to 26°C.

8. A method according to claim 1 or 2, in which the temperature control piping circulation loop to maintain the process temperature of the piping in the range of 5 to 20°C, preferably from 7 to 15°C.

9. A method according to claim 1 or 2, wherein the circulation circuit includes one or more additional homogenizers connected in parallel to the main homogenizer circulation loop.

10. A method according to claim 1 or 2, wherein the homogenizer includes more than one working chamber, where the chambers are connected in parallel.

11. A method according to claim 1 or 2, wherein the nanoemulsion PFOS before standing bottled in consumer packaging.

12. A method according to 11, in which consumer packaging with nanoemulsion PFOS gently shaken until the homogeneity of the content.

13. A method according to claim 11, in which consumer packaging is a glass bottle, vials, ampoules, syringe-tubes, plastic containers, metal containers, tubes.

14. A method according to claim 13, in which the amount of consumer packaging for nano-emulsion PFOS intended for freezing, naprawiam 500 ml.

15. A method according to claim 1 or 2, wherein the nanoemulsion PFOS maintained at a temperature of from 2 to 10°C for not less than 18 hours, preferably at a temperature of 3-6°C for 18 to 30 hours, preferably at 4°C for 24 hours.

16. A method according to claim 1 or 2, wherein the nanoemulsion PFOS freeze.

17. A method according to claim 16, in which a nanoemulsion PFOS frozen at a temperature of from -4 to -18°C.

18. A method according to claim 1 or 2, wherein the aqueous solution of the stabilizing additive is a 10-30 mass. % aqueous solution, preferably 10-20 wt. % aqueous solution.

19. A method according to claim 1 or 2, in which the stabilizing additives are used poloxamer, such as copolymers of polyoxyethylene and polyoxypropylene, S10-C22fatty acids and/or their salts or triglycerides or phospholipids of egg yolk or soybean.

20. A method according to claim 19, in which the stabilizing additives are used poloxamer with the trade names Proxenol 268, Amoxil 268 brand "A", Kolliphor P188 (Califor P188), Pluronic F68 (Pluronic F68), Synperonic F68 (Synperonic F68), Lutrol F68 (Lotrel F68).

21. A method according to claim 1 or 2, in which the mixture of PFOS use a mixture of at least one quickly withdrawn PFOS,
selected from C8-C10PFOS, and at least one slowly withdrawn PFOS selected from C11-C12PFOS.

22. A method according to claim 21, in which quickly withdrawn PFOS choose from performanceline (PFD) or performancebased (PFOB) or their mixtures, and slowly withdrawn PFOS choose from performapply (PPTP), (PFMRP) or pftba (PFTB) or blends.

23. A method according to claim 1 or 2, in which the mixture of PFOS for the preparation of predementia prepared by mixing one or more liquid PFOS.

24. A method according to claim 1 or 2, wherein the water-salt solution comprises NaCl and water and, optionally, KCl, MgCl2, NaHCO3, NaH2PO4, Na2HPO4.

25. A method according to claim 24, in which the water-salt solution further comprises glucose.

26. A method according to claim 1 or 2, wherein the nanoemulsion contains PFOS 4,8-7,2 g/l of sodium chloride; 0.31 and 0.47 g/l of potassium chloride; 0,15-0,23 g/l of magnesium chloride (in terms of dry substance); 0,55-0,83 g/l sodium bicarbonate; 0,16-0,24 g/l sodium phosphate onesemester (in terms of dry substance); 1.6 to 2.4 g/l of glucose.

27. A method according to claim 1 or 2, wherein the pH of the nano-emulsion PFOS is from 5 to 8.0.

28. A method according to claim 27, in which to adjust the pH change of the quantitative content of sodium bicarbonate and sodium phosphate onesemester.

29. A method according to claim 1 or 2, wherein the nanoemulsion PFOS has
the average particle size of not more than 150 nm, preferably from 30 to 100 nm, most pre�respectfully from 30 to 80 nm.

30. A method of producing sterile nano-emulsion performancesin compounds (PFOS), including:
- pre-filling the circulation circuit of an aqueous solution of a stabilizing additive;
- add mixture to PFOS aqueous solution of a stabilizing additive;
- homogenization of the mixture of PFOS from aqueous solution of stabilizing additives with getting predementia PFOS;
- mix predementia PFOS from water-salt solution to obtain nano-emulsion PFOS.



 

Same patents:

FIELD: nanotechnology.

SUBSTANCE: shell of the nanocapsules is used as apple or citrus high- or low-esterified pectin, and the core - as L-arginine. According to the inventive method, L-arginine is suspended in benzene, the resulting mixture is dispersed into a suspension of apple or citrus high- or low-esterified pectin in benzene in the presence of the preparation E472s while stirring 1000 revolutions per second. Then carbon tetrachloride is added, the resulting suspension of the nanocapsules is filtered and dried at room temperature. The process is carried out for 15 minutes.

EFFECT: simplification and acceleration of the process of producing the nanocapsules, and increase in the yield by weight.

6 ex

FIELD: nanotechnology.

SUBSTANCE: method of production of nanocapsules of vitamin in sodium alginate is characterized in that the shell is used as sodium alginate, and the core - as the vitamin, in a weight ratio of core:shell as 1:3. According to the method of preparing the nanocapsules the vitamin is added to a suspension of sodium alginate in benzene in the presence of the preparation E472s while stirring at 1300 rev/sec. Then hexane is added, the resulting suspension is filtered and dried at room temperature.

EFFECT: simplification and acceleration of the process of production of the nanocapsules, and increase in the yield by weight.

3 dwg, 8 ex

FIELD: medicine.

SUBSTANCE: biocompatible implant (BI) made of magnetic material in a biocompatible matrix (BM) is placed loosely along a posterior surface of an injured spinal cord of an experimental animal (EA) The EA is immersed periodically into a constant magnetic field. Its magnetic vector is aligned with a craniocaudal direction of spinal tracts. The BM of the BI is animal or herbal gelatine, wherein ferromagnetic magnetite or ferromagnetic ferrite nanoparticles 18-42 wt % with a particle size of 2.0-38 nm and at a magnetic field intensity (N) of 5-10 mT are immobilised as a magnetic material of the BI. The magnetic exposure covering the traumatic spinal injuries involves the combined effect of the magnetic field of the BI and the external rotating magnetic field at a magnetic induction of 0.15-0.35 T. The external magnetic exposure frequency is 1 or 2 times a day; the length is from 2 to 8 minutes per one session; the number of sessions is from 2 to 4. Animal gelatine in the BM of the BI can represent agar-agar, whereas herbal gelatine is pectin. The BM of the BI can additionally contain polyamines contributing to cell growth and proliferation, e.g. spermine or spemidine, in an amount of 1-5 wt %.

EFFECT: method enables recovering the spinal cord function sufficiently completely in the distal direction from the injury region, providing the favourable conditions for the adequate neuroglial growth with recipient's long neuron penetration from the intact proximal portion of the spinal cord into the distal one to ensure its conductive function, reducing the cicatrisation within the spinal injury, and eliminating the cerebral tissue oedema.

3 cl, 8 ex

FIELD: chemistry.

SUBSTANCE: disclosed catalyst for low-temperature oxidation of carbon monoxide, which is silver deposited on a silicon dioxide surface in amount of 1-16% of the weight of the catalyst. The catalyst contains silver in the form of nanoparticles with a size smaller than 6 nm, which are uniformly distributed on the surface of mesoporous silica gel with specific surface area of 50-200 m2/g and pore size of 3-60 nm, which is used as a support. The invention also relates to a method of using the catalyst to remove carbon monoxide from air, which is carried out using the catalyst by passing a stream of moist air containing up to 100-115 mg/m3 CO through the catalyst bed at room temperature.

EFFECT: improved catalyst properties.

4 cl, 1 tbl, 4 dwg, 5 ex

FIELD: chemistry.

SUBSTANCE: invention can be used in obtaining coatings, reducing coefficient of secondary electronic emission, growing diamond films and glasses, elements, absorbing solar radiation. Colloidal solution of nano-sized carbon is obtained by supply of organic liquid - ethanol, into chamber with electrodes, injection of inert gas into inter-electrode space, formation of high-temperature plasma channel in gas bubbles, containing vapours of organic liquid. High-temperature plasma channel has the following parameters: temperature of heavy particles 4000-5000K, temperature of electrons 1.0-1.5 eV, concentration of charged particles (2-3)·1017 cm3, diameter of plasma channel hundreds of microns. After that, fast cooling within several microseconds is performed.

EFFECT: simplicity, possibility to obtain nanoparticles of different types.

3 cl, 1 dwg

FIELD: chemistry.

SUBSTANCE: invention relates to field of nanotechnologies and can be used for obtaining composite materials with high electric and heat conductivity, additives to concretes and ceramics, sorbents, catalysts. Carbon-containing material is evaporated in volume thermal plasma and condensed on target surface 9 and internal surface of collector 7. Plasma generator 3, which includes coaxially located electrodes: rod cathode 4 and nozzle-shaped output anode 5, are used. Gaseous carbon-containing material 6 is supplied with plasma-forming gas through vortex chamber with channels 2 and selected from the group, consisting of methane, propane, and butane. Bottom of collector is made with hole 8 for gas flow to pass.

EFFECT: invention makes it possible to reduce energy consumption of the process, extend types of applied hydrocarbon raw material, simplify device construction and provide continuity of the process and its high productivity.

2 dwg, 3 ex

FIELD: chemistry.

SUBSTANCE: invention relates to synthesis of diamond nanoparticles, which can be used in various fields of technology. Claimed method of synthesis of ultradispersed diamonds includes generation of carbon plasma from carbon-containing substance and its condensation with cooling liquid under conditions of cavitation. As plasma-generating substance any hydrocarbon gas or organic carbon-containing liquid, including one which additionally contains substances, containing heteroatoms, as well as dispersions of carbon particles of non-diamond allotropic shape in organic fluids or water, can be used. Flow of liquid inside flow cavitation apparatus, providing additional cavitation impact on cooling liquid, is used as cooling liquid.

EFFECT: invention makes it possible to increase energy efficiency of realised synthesis of nanodiamonds and provides possibility of managing properties of synthesised nanodiamonds.

3 dwg, 1 tbl

FIELD: chemistry.

SUBSTANCE: method of obtaining a composite material includes the influence on a mixture of a carbon-containing material, filler and sulphur-containing compound by a pressure of 0.1-20 GPa and a temperature of 600-2000°C. As the sulphur-containing compound applied is carbon bisulphide, a compound from the mercaptan group or a product of its interaction with elementary sulphur. As the carbon-containing material applied is molecular fullerene C60 or fullerene-containing soot. As the filler applied are carbon fibres, or diamond, or nitrides, or carbides, or borides, or oxides in the quantity from 1 to 99 wt % of the weight of the carbon-containing material.

EFFECT: obtained composite material can be applied for manufacturing products with the characteristic size of 1-100 cm and is characterised by high strength, low density, solidity not less than 10 GPa and high heat resistance in the air.

11 cl, 3 dwg, 11 ex

FIELD: chemistry.

SUBSTANCE: preliminary devolatilisation and purification of a rolled film is realised in a mixture of an inert gas and nitrogen with its movement relative to plasma of a magnetron discharge in a vacuum chamber. The application of oxide is carried out in the chamber with, at least, one pair of magnets in the bipolar pack-pulse mode or their electrical power supply with the stabilised voltage and current, limit for the source of electricity.

EFFECT: creation of multilayer polymer films, high-barrier relative to the penetration of gases and vapours.

3 cl, 1 dwg, 1 ex

FIELD: chemistry.

SUBSTANCE: invention relates to method of obtaining L-arginine nanocapsules in sodium alginate envelope. In the process of method realisation L-arginine is suspended in benzene. Obtained mixture is dispersed into suspension of sodium alginate in hexane in presence of preparation E472c with mixing at 1000 rev/sec. After that, chloroform is added, and obtained suspension of nanocapsules is filtered and dried at room temperature. Process is realised for 15 minutes.

EFFECT: method in accordance with invention provides simplification and acceleration of process of obtaining nanocapsules and increased output by weight.

3 ex

Swirling emulsifier // 2556163

FIELD: process engineering.

SUBSTANCE: invention relates to making of fine dispersions in fluid-fluid systems. This emulsifier comprises vortex tube with two tangential pipes. Said tube is composed of cylinder its length being equal to its ten IDs. One of said pipe serves to feed the first component and is arranged at 20-30 degrees to horizontal line while second pipe discharges finished emulsion and is arranged nearby vortex tube lower end, Second component feed pipe is arranged coaxially in vortex tube nearby its lower end for axial displacement while spacing from second component feed pipe end to vortex pipe upper end makes 0.25-1 of second component feed pipe ID. Finished emulsion pipe features ID equal to half the vortex pipe ID, second component feed pipe has inner radius equal to first component feed pipe ID.

EFFECT: lower fluid pressure losses, increased cavitation zone, ruled out dead zones.

3 dwg

Hydrodynamic mixer // 2553861

FIELD: machine building.

SUBSTANCE: mixer comprises a casing with axial and radial branch pipes for component supply, mixing elements. The axial inlet branch pipe can move back-and-forth and is made as a conical-cylindrical nozzle. A mixing element consists of a conical insert with circular grooves on its surface. The insert is located in the conical part of the mixing element casing. A deflector in the form of an indentation is provided at the end face of the insert opposite the conical-cylindrical nozzle in the centre. The mixing element casing is fitted by through channels set on concentrical circumferences. The circular grooves are connected to the first mixing chamber by channels. There shall be at least two mixing elements. The total area of the channels connecting the circular grooves to the mixing chamber amounts to (5-20)% of the area of the cross section of the circular radial gap at the inlet to the mixing element. Diameters of the concentrical circumferences on which centres of the channels provided on the end face surface of the conical insert greater base are located, are determined according to a mathematical expression.

EFFECT: intensified hydrodynamic, physical-chemical and heat-and-mass exchange processes.

4 cl, 3 dwg

FIELD: engines and pumps.

SUBSTANCE: invention proposes an ultrasonic mixer of vegetable oil and mineral fuel, which includes ultrasonic emitter (1) and electronic control unit (3). Ultrasonic emitter (1) is arranged in a cavity of mixer housing (7) having inlet channels (8 and 9). Voltage of an on-board network of automotive equipment (+12 V) is supplied to electronic control unit (3) that shapes and supplies high-frequency signals to the ultrasonic emitter. Vegetable oil and mineral fuel are supplied through inlet channels (8 and 9) to the mixer and mixed under action of ultrasonic vibrations.

EFFECT: treatment of vegetable and mineral components of a mixed fuel by an ultrasound leads to quality mixing and obtainment of a homogeneous fine emulsion.

3 cl, 2 dwg

FIELD: process engineering.

SUBSTANCE: invention relates to the device for preparation of off-shelf putty bulk by binder and hardener in compliance with genus proximus of invention clause 1. This device comprises vessel with bottom cover with outlet, plate-like dash-board fitted on bed plate to make inlets on one of the plate sides to feed the binder and hardener and outlet made at opposite plate side. Dash-board in the area of both outlets has the holder for plug-in attachment of said mixer. This device comprises hand-held drive for controlled feed of both piston rods depending on feed stroke to control required amount of components from vessels to mixer.

EFFECT: simplified and compact design.

28 cl, 48 dwg

FIELD: process engineering.

SUBSTANCE: invention relates to emulsification of oil and water for preparation of water emulsion of gluing agents for bulk or paper and cardboard separately. Continuous phase is fed at pressure through Venturi nozzle into mixing chamber. Disperse phase is optionally fed at pressure in Venturi nozzle mixing chamber. Emulsion produced in mixing chamber is forced through mixed phase nozzle and from Venturi nozzle. Venturi mixed nozzle diameter is larger than that for continuous phase at the ratio higher than 1:1 and lower than 4:1.

EFFECT: stable gluing emulsions, stable operation of apparatus and efficient gluing.

16 cl, 4 dwg, 7 ex

FIELD: oil-and-gas industry.

SUBSTANCE: proposed process comprises oil slime heating and cleaning. Cleaned and heated mix of hydrocarbons with water is fed into working vessel and separated into at least two flows. Separated flows are fed for their homogenisation into vibratory cavitation homogeniser at different flow rate magnitude of at least 1.5. Homogenisation is executed in vibratory cavitation homogeniser with spinning rotor with perforated surface and stationary stator at the mix specific flow rate not over 2.5 g/cm2 of rotor working surface per second and peripheral speed not over 20 m/s. Processing is conducted three times: primary processing is performed unless hydrated fuel emulsion with water drop sixe not over 15 mcm, subsequent processing is conducted unless water drop size does not exceed 5 mcm.

EFFECT: higher stability.

4 cl, 7 ex

FIELD: power engineering.

SUBSTANCE: in the method to prepare fuel oil for burning from the total volume of the main flow of fuel oil they take a volume of side flow of fuel oil equal to 1.5 - 2% of the volume of the main flow of fuel oil. The main flow of fuel oil is sent into the line of the side flow of fuel oil. Heating of the fuel oil flow, mixing of an additive and fuel oil is carried out in the line of the side flow of fuel oil, in parallel connected to the line of the main flow of fuel oil. Mixing of the volume of the side flow of fuel oil and the additive is carried out without participation of movable mechanical facilities by means of a mixer made in the form of a device for static mixing of fuel oil and additive. The additive is supplied into the line of the side flow of fuel oil to the process of static mixing of fuel oil and the additive.

EFFECT: simplified process circuit with preservation of high operational characteristics of fuel oil due to high homogenisation of the introduced additive and fuel oil by means of intensive mixing and automation of preparation of homogeneous mixture of fuel oil and additive, efficient preparation and burning of liquid fuel with release of smaller quantity of toxic substances, increased reliability, cost effectiveness and environmental safety of boiler plants.

4 cl, 1 dwg

FIELD: energy industry.

SUBSTANCE: installation comprises the sources of oil product and water, steam generator, pumps, steam lines, pipelines, water and oil product heaters, rotary machine, holding tank, oil product processing circuits, the system of preparing the metered components, the system of steam distribution, the system of drainage steaming and cleaning the equipment. The first circuit of oil product processing comprises a starting pump which inlet is in communication with a source of oil product and the outlet - with the inlet of the unit of rough cleaning. The outlet of the unit of rough cleaning is in communication with the inlet of the first pre-heaters of the cascade of the pre-heaters of oil product. The outlet of the last pre-heater is in communication with the intermediate damping capacity. The second circuit of oil product processing comprises a finishing pump which inlet is in communication with the first outlet of the intermediate damping capacity, and the outlet is in communication with the inlet of the first unit of fine cleaning. The second circuit of oil product processing comprises a preliminary mixer which outlet through the second unit of fine cleaning is in communication with the inlet of the rotary device. The system of preparing the metered components comprises a unit of oil product metering which inlet is in communication with the outlet of the first unit of fine cleaning and is equipped with a water pre-heater which inlet through the shut-off valve is in communication with a source of water, and the outlet through the unit of water metering is in communication with the inlet of the water pump, which outlet is in communication with the inlet of the third unit of fine cleaning. The outlet of the third unit of fine cleaning and the outlet of the unit of oil product metering are in communication with the inlet of the preliminary mixer. The steam generator is in communication with the heat transfer elements of the filters of rough and fine cleaning, the pre-heater and the water pre-heater. The cavities of filters of units of rough cleaning and the first and second units of fine cleaning, the pre-heater, the water pre-heater and the rotary machine with the modulation of flow are in communication with the steam generator.

EFFECT: high performance.

15 cl, 2 dwg

Emulsor // 2502549

FIELD: process engineering.

SUBSTANCE: invention relates to food industry, particularly, to making multicomponent mixes with addition of liquid ingredients. Two inclined feed flutes with their bottoms heated by hot water are mounted at top part of rectangular housing. Fluid components atomisers are arranged under said flutes at two opposite sidewalls. Rectangular housing is mounted vertically at trough-shape mixer. Mixer comprises two serial chambers, one arranged above said rectangular housing and another one at conical part extending beyond said housing. Low- and high-speed shafts are arranged coaxially inside said mixer. Note here that low-speed shaft is fitted inside high-speed shaft to extend through two chambers while high-speed shaft passes through first chamber only. Mixer taper section has dual casing with cold water feed and discharge pipes. Two different-diameter ribbon coils and driving screw are fitted on mixer high-speed shaft. Variable-pitch-and-diameter taper driving screw is fitted on low-speed shaft.

EFFECT: higher homogeneity.

1 dwg

FIELD: process engineering.

SUBSTANCE: invention relates to heat engineering and may be used for making water-fuel emulsions for industrial boilers, marine engines etc. Proposed device comprises fuel storage tank, emulsifier and pipelines to communicate both. Said emulsifier has two inlet pipes. Said storage tank has two intake pipes connected with emulsifier inlet pipes, one being fitted inside said tank through 1/3 of tank length and another one through 2/3 thereof. Emulsifier pressure pipe is communicated with tank top nearby rear bottom. Filters are arranged at intake pipe lines. Rotary mixing disperser makes said emulsifier.

EFFECT: power and time savings.

3 cl, 2 dwg

FIELD: medicine.

SUBSTANCE: for patient with alimentary obesity preliminarily determined are: clearance of osmotically free water (CFW) and colloid oncotic pressure (COP). If CFW level is lower than -0.45 ml/min and COP is lower than 18 mm Hg, therapy starts from intravenous introduction of 6% HES "Voluven" in dose 6.5 ml/kg/day at rate 350 ml/h. Immediately after the end of infusion introduction with syringe doser of 25% solution of magnesium sulphate in dose 0.75 ml/kg/day at rate 2.8 ml/h. For patient without obesity, if CFW level is lower than -0.6 ml/min and COP is lower than 21 mm Hg, therapy is started from intravenous introduction of 6% HES "Voluven" in dose 5.5 ml/kg/day at rate 300 ml/h. 25% solution of magnesium sulphate in dose 0.9 ml/kg/day at rate 2.4 ml/h is also introduced by means of syringe doser. Treatment is carried out during a day.

EFFECT: carrying out adequate therapy in said category of patients due to selection of mode of introduction of preparations, conditioning fast recovery of kidney function.

2 cl, 2 ex

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