Method of correction of immunobiochemical status in cows in prenatal and postpartum periods

FIELD: veterinary medicine.

SUBSTANCE: invention is a method of correction of immunobiochemical status of cows in the prenatal and postpartum periods. The invention comprises the following steps: the dry cows within 10-12 days prior to and 10-12 days after parturation are given orally the composite agent based on organic acids, containing fumaric, ascorbic, succinic and citric acid based on per dose, mg/kg body weight: fumaric acid - 3.0-5.0; ascorbic acid - 4.5-7.5; succinic acid - 6.0-10.0; citric acid - 1.5-2.5, in combination with dualfold intramuscular injection tetrahydrovit 30 days before and on the day of parturation at a dose of 10 ml/head, and threefold intramuscular injection of iron complex 30, 15 days before and on the day of parturation at a dose of 0.2 ml/10 kg bodyweight.

EFFECT: invention provides a correction of immunobiochemical homeostasis at the level providing for physiological parturation and the postpartum period and obtaining viable offspring.

14 tbl, 3 ex

 

The invention relates to veterinary medicine, relates to a method of correction immunobiochemical status of cows during the prenatal and postnatal periods and can be used for the treatment and prevention of cow protection from mass diseases of the reproductive organs and produce physiologically healthy offspring.

Pregnancy, following childbirth and the puerperium all mammals are accompanied by substantial changes in metabolism that may occur in clinical and subclinical metabolic disorders. The most vivid picture of the intensity of metabolic processes is formed at the final stage of pregnancy and the early postpartum period. Furthermore, it was reported that the basis for the development of obstetric pathology in animals is the disorder of the synthesis and metabolism of hormones, vitamins, proteins, carbohydrates, lipids, minerals and other biologically active substances. High physiological role of trace elements in the animal body characterized by the fact that they are closely associated with activation of hormones, enzymes, vitamins and other biologically active substances (BAS) that support homeostasis. Trace elements actively bind to the proteins, resulting in formation of new substances, which have high activity in the body. If the body does not grip�tons of minerals, the formation of hormones, vitamins and enzymes is significantly reduced and, accordingly, reduces the overall metabolism. A significant number of enzymes closely related to micronutrients. Thus, manganese is an active part of the enzyme arginase, which break down proteins. Trace elements stimulates the synthesis and activity of various vitamins, and in some of them they are included as components. For example, vitamin b12contains cobalt. Effect of trace elements on the body are made mainly by enzymes, vitamins and hormones. Increasing their activity, trace elements significantly improve the overall metabolism of proteins, carbohydrates, lipids, etc., increased breathing, better blood formation, synthesis of proteins and the absorption of feed nutrients, allowing increased growth and development of animals, increases their productive and reproductive performance. Vitamins and minerals play an important role in maintaining homeostasis in cows. So, zinc is involved in the metabolism of nucleic acids, carbohydrates, fats and proteins, is of great importance in the immune system and is part of the hormones. Iron is involved in redox processes. It is part of hemoglobin and some enzymes (oxidases, peroxidases, catalase), Chi�Le cytochrome, involved in biological oxidation. Manganese is involved in the formation of connective tissue and cartilage, functioning reproductive functions necessary for lipid and cholesterol metabolism, prevents fatty degeneration of the liver. Copper is an essential trace mineral involved in metabolism and tissue respiration, blood formation, is part of hormones and enzymes, has anti-inflammatory action. Cobalt is part of cyanocobalamin, promotes the release of water by the kidneys, blocking and removal of toxic elements from the body. Selenium protects the body from harmful substances and toxins, stimulates the metabolism and the body's immune defenses. Iodine regulates the speed of chemical reactions is part of thyroid hormones. Vitamin a plays an important role in the growth and reproduction of cells, provides a normal mucous membranes (barrier function), supports the function of the retina and the process of light perception. Vitamin D participates in the regulation of calcium-phosphorus metabolism. Vitamin E normalizes the functions of the organs of reproduction (promotes the fertilization and development of embryos), influences the activity of the pituitary gland and the thyroid gland, destroying the most reactive forms of oxygen and therefore prevents the destruction of the Pauline�asimenia fatty acids [1].

Disorders of mineral metabolism often lead to a weakening of the immune system, reproductive disorders and fertility [2]. When latent deficiency of macro - and microelements, in violation of the ratio of minerals in the body, the disease is no apparent clinical signs.

Not the last role in connection with the occurrence of various pathological conditions of peroxide oxidation of lipids. Currently it is proved that the processes of lipid peroxidation not only play an important role in normal physiology and biochemistry of cells, but also act as a universal link of nonspecific mechanisms of development of various pathologies, and depending on the level of their activity can turn into a key factor of pathogenesis of diseases.

The status value immunobiochemical status as a pathogenic factor of obstetric pathology cows is confirmed by the low efficiency of methods of prevention with the use of drugs excluding immunobiochemistry processes.

Known method of correcting abnormalities of metabolism, treatment and prevention of their consequences, including the use of universal complex preparation containing sodium Selenite, vitamin E, succinic acid, sulphate of zinc and formazin, which is administered intramuscularly �and 45, 30, 15 days before delivery and on the day of birth [3].

Another known means of increasing reproductive capacity of farm and domestic animals on the basis of various biologically active compounds, positively influencing reproductive function, improves circulation, on the basis of micro - and macroelements and vitamins [4]. As a result of the correction of metabolic processes occurs metabolic reorientation of the body that have a significant impact on the level of natural resistance.

Also known means that provides antioxidant protection, promotes normalization of metabolism and increases the resistance due to the reduction and prevention of accumulation of toxic products of lipid peroxidation, such as a preparation containing succinic and ascorbic acid [5].

As the preferred analogue is a method of normalizing immunobiochemical status of the cows in the prenatal and postnatal periods, including a single intramuscular injection of 0.5% solution of sodium Selenite for 20-25 days before delivery, two doses before and two after childbirth with an interval of 10 days, terravita containing vitamins a, D3, E and C. it is administered orally for 20-25 days before and after childbirth succinic acid [6].

However, the known methods do not provide normalization immunol�khimicheskogo status in full, exposing selected indicators without taking into account the mechanisms of disorders of metabolism depending on the physiological state of the animal.

Technical solution to the problem is to increase the immune response of animals in the prenatal and postnatal periods, improved intrauterine fetal growth and security of the complex effect of BAS on the maximum number of protective devices in the body, including antioxidant defense system.

The problem is solved in that in the method of correcting immunobiochemical status of cows during the prenatal and postnatal periods, consisting in the appointment of a down-calving cows organic acid, vitamin and mineral complexes, organic acid used in the form of composite tools, dubbed "Bio-FAIAL" containing fumaric, ascorbic, succinic acid and citric acid, as vitamin complex - drug tetrahydrof, as a mineral complex - complexului.

The method is as follows.

Cows within 10-12 days before and 10 to 12 days after birth oral use compositional tool "Bio-FAIAL" containing fumaric, ascorbic, succinic acid and citric acid based on the dose, mg/kg of body weight: fumaric acid - 3,0-5,0; ascorbic acid - 4,5-7,5; amber� acid 6,0-10,0; citric acid and 1.5 - 2.5, in combination with a double intramuscular injection of tetrahedrality 30 days before and on the day of birth at a dose of 10 ml/goal. and three-time intramuscular injection complexului 30, 15 days before and on the day of birth at a dose of 0.2 ml/10 kg of live weight.

Composite agent based on organic acids, called "Bio-FAIAL", contains substances (fumaric, ascorbic, succinic acid and citric acid), possessing membrane-protective and immunity-boosting properties. Succinic acid and citric acid are important factors in the regulation of physiological condition and improve the overall resistance of the organism of animals and birds that activate the nervous system, kidney and intestine, have anti-stress effect. Succinic and citric acids have the properties of antidotes, cleansing the body from heavy metals, radionuclides, salts of nitric and nitrous acids. In addition, succinic acid has lactoproteins and antiviral action. Citric acid stimulates the formation in the intestine chelated forms of calcium, zinc, iron, etc., which are much easier to assimilate. It is known that citric acid acts as a synergist with amber, strengthening the biological effects of the latter. Ascorbic acid (vitamin C) plays an important role in the Oka�inflammatory-regenerative processes, reactions of carbohydrate and protein metabolism, lowers cholesterol and calcium in the blood, reduce the deposition of lipids and calcium in the organs, activates enzymes of the stomach, intestines, has antihemorrhagic effect, prevents the development of infection in the body, stimulates exocrine pancreatic function, erythropoiesis, and mononuclear phagocytes, provides anti-inflammatory effect ionized calcium, activates phagocytosis. Fumaric acid is a natural metabolite of living organisms, provides extra ATP synthesis, thereby counteracting the stresses at the cellular level. It is resistant to oxidation, temperature and compatibility with other BAS. The accelerated formation of ATP with the participation of fumaric acid contributes to the conservation of glucose for glycogen synthesis in the liver and ascorbic acid in the body. Accumulation in the liver of spare nutrients and ascorbic acid prolongs the period of active functioning and provides a cleansing of the liver from metabolic products.

Tetrahydrof - complex 4 vitamins containing 1 ml: vitamin A - 25000 ME; vitamin D3- 5,000 IU, vitamin E 25 mg, vitamin C - 50 mg, whereas deficiency of vitamins in animals [7].

Complexi�Lesa - a medicament in the form of solution for injection, intended for the prevention and treatment of iron deficiency anemia, lack of trace elements, the normalization of metabolism, increase productivity and safety of livestock of cattle, which includes complex ethylenediaminetetra acid and lysine iron, manganese, copper, zinc, cobalt, selenium, iodine and water for injections. In 1 ml of solution contains as active ingredients, mg: iron - 10,0; manganese - 7,0; copper 1,0; zinc - 9,0; cobalt and 0.12; selenium - 0,11; iodine - 0.26 in the form of chelates and water for injections [8].

The inventive method is illustrated by examples.

Example 1. To study the effect of compositional means "Bio-FAIAL" immunobiochemical status on the principle of steam-analogues picked up four groups of down-calving cows for 15-20 days before giving birth: 3 groups, group 1 - control. All animals were housed under identical conditions of feeding and maintenance.

Cows first experimental group (n=9) was fed in mixture with forage compositional tool "Bio-FAIAL, containing per dose, mg/kg of body weight: fumaric acid - 4,0; ascorbic acid 6,0; succinic acid - 8,0; citric acid - 2,0; cows second experimental group (n=8) is a compositional tool "Bio-FAIAL, containing per dose, �g/kg live weight: fumaric acid 5,0; ascorbic acid - 7,5; succinic acid - 10,0; citric acid, 2.5; cows of the third experimental group (n=10) is a compositional tool "Bio-FAIAL, containing per dose, mg/kg of body weight: fumaric acid - 7,0; ascorbic acid - 10,5; succinic acid - 14,0; citric acid - 3,5; within 10-12 days before and within 10-12 days after birth. Animals of the fourth group (n=10) of the drugs were received and served as the control.

To assess immunobiochemical status of the experimental animals was carried out blood tests before you start experience - 15-20 days before the birth and after 10-12 days after birth. Immunobiochemical status was assessed by morphobiochemical indicators (table 1), indicators of natural resistance (table 2) and immune status (table 3).

The animals were observation, noted the nature of the prenatal, descent and post-Natal periods, conducted clinical and gynaecological examinations cows, take into account the timing of involution of the reproductive organs and insemination. The results are presented in table 4.

When analyzing the results immunobiochemistry of blood tests (tables 1, 2, 3) found that a compositional tool "Bio-FAIAL" not an eye�Ivalo negative influence on the studied parameters. Most physiological dynamics immunobiochemistry rates observed in cows of the first and second experimental groups. As seen from table 4, all the cows of the control group was observed pathology childbirth and the postpartum period. The use of composite tools "Bio-FAIAL" experimental animals has reduced the incidence of reproductive organs in cows at birth and the postpartum period 20.0-37.5% in comparison with the control, a significant shortening of involution of the genital organs after birth in cows of the first and second experimental groups by 7.0 and 10.3 days, respectively, in comparison with the control group, a significant decrease in the number of days of infertility and duration of the service period by 12.6 days and index fertilization 26.6% in cows of the second experimental group. Fertility of cows in the experimental groups compared with the control was above 10.0-18.9 per cent. Studies have confirmed the validity of the claimed doses composite tools "Bio-FAIAL".

Example 2. To justify the industrial applicability of the claimed method on the principle of steam-analogues picked up four groups of heavily pregnant cows 30 days prior to calving: 3 groups, group 1 - control. Cows of the experimental group was administered: the first group compositional tool "Bio-FAIAL, containing per dose mg/kg of body m�Ref: fumaric acid 5,0; ascorbic acid - 7,5; succinic acid - 10,0; citric acid, 2.5, during the 12 days before and 12 days after birth (oral), complexului in a dose of 0.2 ml/kg of body weight for 30 and 15 days before and on the day of birth (intramuscularly), tetrahydrof in the dose of 10 ml/goal. 30 days before and on the day of birth; the second group complexului in a dose of 0.2 ml/kg of body weight for 30 and 15 days before and on the day of birth (intramuscularly), tetrahydrof in the dose of 10 ml/goal. 30 days before and on the day of birth (intramuscularly); the third group compositional tool "Bio-FAIAL, containing per dose mg/kg of body weight: fumaric acid - 5,0; ascorbic acid - 7,5; succinic acid - 10,0; citric acid, 2.5, during the 12 days before and 12 days after birth (oral). The cows of the control group drugs were prescribed. The blood sampling was performed before the experiment for 30-35 days before the birth and after 10-14 days after birth. The findings were calculated as in example 1. The results are presented in tables 5, 6, 7, 8. Born calves were weighed on the first day after birth and on the 30th day of life and were evaluated according to the main clinical indicators. The results are presented in tables 9, 10, 11.

Studies have shown that the inventive method is provided for maintaining at an optimum level after the birth of total lipids, vitamin A (table 5) and bactericidal activity of blood serum, increased lysozyme activity of serum - 17.7% (table 6), Ig G - by 25.7%, Ig A - 40.0%, Ig M - 52.2% (table 7).

As seen from table 8, the incidence of cows in first, second and third experimental groups was lower than in controls by 55.6; 7,8 and 48.8%, respectively. More physiological for the generic and the postpartum period in animals of the first experimental group was led to the shortening of involution of the genital organs 18.7 days (p≤0,001) compared to 4 days for the second and 14.6 days (p≤0.001) in the third experimental groups, reducing the duration of infertility 16.6 days (p≤0,001) compared to 8.5 for the second and 13.7 days (p≤0.01) in the third experimental groups and index of fertilization by 25.6% (p≤0.05) compared with 8.4% in the second and 20.0% in the third experimental groups, respectively, compared to control. Fertility in animals of the experimental groups was at the level 80,0 is 88.9%, which is 3.3-22.2 percent higher than in the control. In the first and third experimental groups, there were no registered cases of detention of the placenta, whereas in the second experimental and control groups with retention of the placenta was 20.0 and 20.2% of cows, respectively. In addition�about, two cows of the control group recorded stillbirth.

As can be seen from table 9, at 3-7 days after birth hemoglobin in calves, obtained from cows of first, second and third experimental groups was higher than control group on 45,3 (p≤0,001) and 44.9 (p≤0,001) and 60.6% (p≤0.001) respectively. The level of hemoglobin in calves of the control group amounted to $ 75.9±1.6 g/l, considerably below the physiological norm and indicated a state of hypoxia. The number of red blood cells in all experimental calves was consistent with physiological norm, while in animals of the first experimental group, the rate was significantly higher in comparison with the second group 15.8 (p≤0.05). Glucose levels in all experimental calves were characterized by high performance that was consistent with physiological norm in the early postnatal period (4,0-5,8 mmol/l), while the experimental calves indicators of glucose were higher than those of control calves. The copper content in the serum of calves first and second experimental groups exceeded the value of the corresponding indicator of the third experimental and control groups 11.4 (p≤0.05) and 8.3% (p≤0.05) and 14.7 (p≤0.01) and 11.5% (p≤0.05), respectively. The concentration of substances of low and medium molecular weight (lmmws level) in all animals except calves the second experimental group was at the level of 15.7 compared to 16.1% In young second experimental group IP�ledway index was 18.3±0,2 units, that 13.7 and 17.3% (p≤0,001) higher than in calves of other groups. The best indicators lmmws level recorded in the first and third experimental groups of 15.7±0,2 and 15.6±0.3 units., testified to the decrease in the level of endogenous intoxication as in cows and of their offspring. As seen from table 10, the bactericidal activity of blood serum in all experimental calves was relatively high, however, in the experimental groups the rates were higher than the 3.5 to 11.2%. Phagocytic index in calves of the first group exceeded the value of the corresponding parameter in the control group 22.2% (p≤0.05) and the retinol content was higher than the control 28.7% (p≤0,05).

As seen from table 11, no significant difference in live weight of newborn calves in groups was not, except for the second experimental group, in which the live weight of calves at birth was higher by 8.8% (p≤0,05) in comparison with the control. A month after the birth of the live weight of calves of the first and third experimental groups was higher than that of control animals, 11.0 (p≤0.01) and 8.0% (p≤0.05), respectively. Absolute average daily live weight gain in calves the first and third experimental groups made up 413.3 and 400.0 g, 26.5 and 22.4% higher than in the control.

As seen from table 12, the incidence of calves in the first experimental group was 22.2% compared with 50.0% of the second, to 30.0% in the third experience�'s group and 71.4% in the control. The first calves of the experimental group the first signs of gastro-intestinal diseases were observed on day 7-8 of life, and in calves of the control group - 2-3 days after birth. The disease in calves of the control group was characterized by more severe course. Calves experimental groups (first and third) were pereboleli overwhelmingly mild, the treatment was carried out by the appointment of a diet and feeding rehydration solution and binders. Safety in these groups was 100%.

The results confirmed that the combined use of compositional tools "Bio-FAIAL", complexului and tetrahedrality declared by the scheme helped correct immunobiochemical homeostasis at the level providing for physiological childbirth and the postpartum period and obtaining viable offspring with stronger vigor.

Example 3. To determine the comparative effectiveness declared and known methods according to the principle of analogues were selected four groups of heavily pregnant cows 30 days before the expected birth: 3 groups experimental, 1 control group. Cows of the experimental group was administered: the first group compositional tool "Bio-FAIAL, containing per dose mg/kg of body weight: fumaric acid - 5,0; ascorbic acid - 7,5; succinic acid - 10,0; citric acid, 2.5, over a 12 day� before and 12 days after birth (oral), complexului in a dose of 0.2 ml/kg of body weight for 30 and 15 days before and on the day of birth (intramuscularly), tetrahydrof in the dose of 10 ml/goal. 30 days before and on the day of delivery (the claimed method); the second group - 0.5% solution of sodium Selenite at a dose of 10 ml/goal. 20 days before birth (intramuscularly), terabit in the dose of 10 ml/goal. twice with an interval of 10 days before and after childbirth, succinic acid at a dose of 1.0 g/bird. and for 20 days before and after childbirth (oral) (similar); the third group - 0.5% solution of sodium Selenite at a dose of 10 ml for 20 days before delivery (intramuscularly), terabit in the dose of 10 ml twice with an interval of 10 days before and after childbirth (intramuscularly) (basic method). The cows of the control group drugs were prescribed. The light of the results, as in example 1. The results are presented in tables 13 and 14.

As seen from table 13, the inventive method has provided a more effective correction immunobiochemical status: in all examined parameters showed an increase with the exception of hemoglobin and the level of phagocytic activity of neutrophils, at the same time, these indicators were within physiological norms. The optimization of metabolic processes, increase overall natural resistance and immune status of cows contributed to the decrease in the level of obstetric pathologies 47.8% comparedwith the control, 17.8% compared with the baseline method and by 7.8% compared to the method-analogue, to reduce the duration of infertility 16.6 days in comparison with control, 8.1 days compared with the baseline method and 2.4 days compared to the method-analogue, the increased fertility by 28.9% compared with control, by 18.9% compared with baseline and with the method-analogue (table 14).

High efficiency of the method is related to the combined action and the best selection of dosages and patterns of use of the composite funds "Bio-FAIAL" on the basis of organic acids, complex vitamins in the form of the drug tetrahydrof and mineral complex in the form complexului, each of which has a broad spectrum of pharmacological activity.

Sources of information taken into account

1. Birch, T. T. Biological chemistry. - M.: Medicine, 1998. - 704 S.

2. Zinchenko, L. I., Pogorelov I. E. Mineral and vitamin nutrition of cows. - Leningrad: Kolos, 1980, 77 p.

3. RF patent №2268033 C2, 2006.

4. RF patent №2392824 C1, 2010.

5. RF patent №2289402 C2, 2006.

6. RF patent №2252767 C1, 2005.

7. www/vettorg.net/pharmacy

8. www/vetlek.ru/reestr/item.php

The method of correcting immunobiochemical status of cows during the prenatal and postnatal periods, consisting in the appointment of a down-calving cows organic acid, vitamin and mineral complexes, characterized in, Thu� fumaric, ascorbic, succinic acid and citric acid is used in the form of composite products containing fumaric, ascorbic, succinic acid and citric acid based on the dose, mg/kg of body weight: fumaric acid - 3,0-5,0; ascorbic acid - 4,5-7,5; succinic acid - 6,0-10,0; citric acid - 1.5 to 2.5, as a vitamin complex - drug tetrahydrof, as a mineral complex - complexului, according to the following scheme: composite product containing per dose mg/kg of body weight: fumaric acid - 3,0-5,0; ascorbic acid - 4,5-7,5; succinic acid - 6,0-10,0; citric acid and 1.5 - 2.5 for 12 days before and 12 days after birth (oral), complexului - in a dose of 0.2 ml/kg of body weight for 30 and 15 days before and on the day of birth (intramuscularly), tetrahydrofur - dose 10 ml/goal. before and on the day of birth (intramuscularly).



 

Same patents:

FIELD: medicine.

SUBSTANCE: minor amputation of the foot with the further necrectomy is performed. After the application of an antimicrobial bandage and drainage, the wound is hermetised from the environment by the creation of a negative pressure above the wound in a combination with drug treatment. The reatment is performed in two steps. At the first step the wound with the antimicrobial bandage and drainage is first hermetised from above with an adhesive film, with the creation and support of the negative pressure not lower than 80 mm Hg. Urokinase 500000 U is additionally introduced daily intravenously by drop infusion per 100 ml of physiological solution, Vessel-Due-F in a dose of 600 LU per 100 ml of physiological solution and VAP 20 - alprostadil in a dose of 40 mcg per 100 ml of physiological solution. In addition Antistax in capsules is introduced to the patient. At the second stage active 24-hour vacuum aspiration with the change of the negative pressure from 10 to 80 mm Hg within a day is carried out. Additionally introduced is Vessel-Due-F in a dose of 1 capsule with 250 LU 2 times per day between meals and Antistax. At the first and second stages Antistax is introduced in a dose of 2 capsules in the morning 30-40 minutes before meal, daily. Duration of each stage constitutes not less than 7 days.

EFFECT: increase of the treatment efficiency due to the complete and timely purification of the wound from pathological exudates, elimination of the progression of the purulent-necrotic process, increase of the regenerative activity of tissues, activation of local immunity, recovery of microcirculation and oxygenation of the affected tissues.

2 cl, 2 ex

FIELD: medicine.

SUBSTANCE: what is presented is a group of inventions; it involves a method for activating the health recovery of an involved respiratory epithelial cell an injury of which is related to cough, post-viral or -bacterial infection, acute/chronic bronchitis or chronic obstructive pulmonary disease (COPD) by administering a compound of formula and/or formula , a composition for the same application on the basis of the above compound, a respective method of treating an inflammatory respiratory condition, a pharmaceutical composition for treating the inflammatory respiratory condition, and using the compound of formula (1) and/or formula (2) for preparing the therapeutic agent for treating cough, post-viral or -bacterial, acute/chronic bronchitis or COPD.

EFFECT: health recovery of the respiratory cell for 48 hours as shown by sialylated glycoconjugates on its surface after treatment with influenza virus or bacterium neuraminidase, as well as reducing a rate of cough in guinea pigs sensitised with citric acid solution.

10 cl, 37 ex

FIELD: medicine.

SUBSTANCE: invention represents an irrigation solution containing sodium chloride, mono- and dibasic sodium phosphate, glycosaminoglycans, glucose or its derivatives, water-soluble cellulose or its derivatives, neuraminic acid, an antihypertensive agent and water.

EFFECT: irrigation solution is balanced; it possesses the anti-inflammatory, anti-oedemic and antihypertensive properties.

3 ex

FIELD: medicine.

SUBSTANCE: present invention generally refers to immunology, particularly to bolstering the immune system in the elderly age and represents a composition. The composition for treating and preventing the diseases related to the altered immune system specified in a group consisting of infections, particularly bacterial, viral, mycotic and/or parasitic infections; the autoimmune diseases; the inborn immunity diseases, e.g. NK cell deficiency, phagocyte deficiency, such as leukocyte adhesion deficiency, cyclic neutropenia; and combinations thereof containing a protein fraction comprising acetylneuraminic acid related to a threonine-rich peptide/protein frame.

EFFECT: invention provides bolstering the immune system more effectively.

13 cl, 2 dwg

FIELD: medicine, pharmaceutics.

SUBSTANCE: invention refers to pharmaceutical industry and represents a composition for treating wounds, ulcers, erosions, burns, freeze burns adhesions, as well as infections caused by the gram-positive and gram-negative bacteria Staphylococcus spp., Streptococcus spp., Enterococcus spp., Shigella spp., Escherichia spp., Salmonella spp., Proteus spp., Acinetobacter spp., Citrobacter spp., Pseudomonas spp., Serratia spp., Klebsiella spp., Antracoides spp., Cryptococcus spp., pathogenic fungi of the genera Microsporum, Trichophyton, Nocardia, Aspergillus, yeast-like fungi of the genus Candida (including the multiresistant strains), as well as Actinomycetes and some pathogenic protozoa (Entamoeba histolytica, Trichomonas vaginalis), containing an active ingredient in the form of 0.001-5.0 wt % of collagenase, 0.001-5.0 wt % of lysozyme and/or 0.001-5.0 wt % of sangvitirine, and an carrier in the form of 0.05-1.0 wt % of p-cyclodextrine or liposomes, and acceptable excipients.

EFFECT: invention provides stability and maintained enzymic activity for a long period of time, deep penetration into an affected skin area, potentiated complex antimicrobial and anti-adhesion action.

2 cl, 10 ex, 4 tbl

FIELD: medicine, pharmaceutics.

SUBSTANCE: claimed are: synthetic nutritional composition, suitable for application in prevention of secondary infections after viral infection, characterised by neuraminidase activity; said composition contains sialylated oligosaccharide, selected from group, consisting of 3' cilalyllactose, 6' cilalyllactose and their combination and component, selected from group, consisting of N-acetyllactosamine, N-acetyllactosamine-containing oligosaccharide and their combination and its application for obtaining medication or therapeutic nutritional composition for prevention of secondary infection after viral infection, characterised by neuraminidase activity. It is shown that efficiency of viral neuraminidases can be reduced by introduction of excessive sialylated oligosaccharides thus reducing part of decialirated surfaces of epithelial cells until neutral oligisaccarides, which imitate preferable epithelial sites of binding pathogenic bacteria, are supplied in plewnty.

EFFECT: combined introduction of sialylated oligosaccharides and N-acetyllactosamine and/or N-acetyllactosamine-containing oligosaccharide is especially efficient in prevention secondary viral infections (otitis media) after such infection as influenza as a result of destruction of synergy between action of viral and bacterial pathogens.

13 cl, 1 tbl, 1 ex

FIELD: medicine, pharmaceutics.

SUBSTANCE: invention refers to pharmaceutical industry, particularly to a composition for treating rheumatoid arthritis. Compositions for treating rheumatoid arthritis containing saligenin or Salix ssp extracts, Andrographolide - enriched Andrographis paniculata extract, N-acetyl-glucosamine, and glucuronolactone or glucuronic acid. The use of a combination of saligenin or Salix ssp extracts, and Andrographolide - enriched Andrographis paniculata extract, N-acetyl-glucosamine, and glucuronolactone or glucuronic acid for preparing a drug for treating rheumatoid arthritis.

EFFECT: composition and combination are effective for treating rheumatoid arthritis.

7 cl, 3 ex

FIELD: medicine, pharmaceutics.

SUBSTANCE: invention refers to pharmaceutical industry, particularly to polyvitamin kit for women. The polyvitamin kit for women containing herbal microelements and micronutrients and comprising 2 formulations; the first formulation potentiates an oestrogen phase of menstrual cycle; the second form potentiates a progesterone phase of menstrual cycle with the first and second formulations containing specific ingredients taken in specific proportions.

EFFECT: kit is effective for stimulating menarche in disturbed menstrual function formation, rehabilitation of female adolescents and adults of reproductive age with menstrual dysfunction for preventing and treating ovarian-menstrual irregularities.

7 cl, 2 dwg, 2 tbl, 8 ex

FIELD: medicine.

SUBSTANCE: invention relates to medicine, namely to traumatology and orthopedics and can be applied in complex treatment of posttraumatic, dystrophic, neurological, degenerative and scar-adhesion processes in joint area, deforming osteoarthrosis of large joints and osteochondrosis. Method of treatment includes introduction of drug mixture by ultraphonoresis for 5 minutes on a field, daily. To obtain said mixture applied are ointment "Indomethacyne", "Chondroxyde", gel "Essaven" in proportion 1:1:1, in dose, expressed in form of 3-10 cm long stripe of each ointment. These components are mixed with 50 mg of medication Karipasim, dissolved in 5 ml of physiological solution and 5 ml 2% solution of Trental. Treatment course includes 15-20 procedures.

EFFECT: method ensures fast achievement of antihydrophic and analgetic effect, reduction of total terms of patients' rehabilitation, increase of disease remission periods.

3 ex, 1 tbl, 4 dwg

FIELD: medicine.

SUBSTANCE: invention refers to medicine, namely to obstetrics and gynaecology. The method for prevention of adhesive process in peritoneal cavity in obstetric-gynaecologic surgeries involving administration of chitosan compounds and differing that the compound is applied in layer-by-layer suturing of an operative wound in preperitoneal and skin structure of anterior abdominal wall. The compound represents a sponge measured 60×100×4 mm to 240×180×4 mm and containing 2% collagen acetate, 2% chitosan ascorbate of degree of deacetylation 95% and more, molecular weight 100 to 700 kDa, ascorbic acid - 1.8 g/g of dry chitosan, chondroitin-sulphuric acid - 5-100 mg/g of dry chitosan, hyaluronic acid - 10-100 mg/g of dry chitosan, heparin - 2.5-5 mg/g of dry chitosan and bovine serum growth factor "Adgelon" - 11-220 mkg/g of dry chitosan.

EFFECT: method improves effectiveness of prevention of postoperative adhesive process in small pelvis in women, prevents development of exudative processes in the operative wound tissues and accelerates the healing.

2 tbl, 1 ex

FIELD: medicine, pharmaceutics.

SUBSTANCE: invention relates to the field of medicine and describes a method of obtaining ferrocene microcapsules, where xanthan gum is used as an envelope for the microcapsules, characterised by the fact that a suspension of 100 mg of ferrocene in 2 ml of benzene is dispersed into a suspension of xanthan gum in the presence of 0.01 g of E472 c preparation with mixing, with the addition of 5 ml of acetone and 0.5 ml of water; the obtained suspension is filtered and dried at room temperature.

EFFECT: invention provides the simplification and acceleration of the process of obtaining the microcapsules and increase of output by weight.

1 dwg, 2 ex

FIELD: chemistry.

SUBSTANCE: invention relates to mixed cobalt (II) salts of ketocarboxylic and mercaptocarboxylic acids of general formula (I):

where R=Alk, R'=H, Alk, NH2, NHCOCH3, m=0-3, R"=H, Alk, COOH, n=0-3, where Alk=alkyl C1-C3, or to such compounds as a cobalt (II) salt of mercaptoacetic acid and pyruvic acid, a cobalt (II) salt of mercaptoacetic acid and α-ketoglutaric acid, a cobalt (II) salt of N-acetyl-L-cysteine and pyruvic acid, a cobalt (II) salt of α-ketoglutaric acid and L-cysteine, a cobalt (II) salt of pyruvic acid and 2-mercaptopropionic acid or hydrates or solvates thereof. A method of producing salts of general formula (I) is also disclosed.

EFFECT: invention enables to obtain mixed cobalt (II) salts of ketocarboxylic and mercaptocarboxylic acids, having cyanide antidote activity.

7 cl, 7 ex

FIELD: chemistry.

SUBSTANCE: invention relates to antifungal preparation, containing effective quantity of methyl ether of 2-benzimidazolylcarbamic acid, or its salts with inorganic and organic acids, or their hydrates, or its complex compounds with organometallic and inorganic salts, containing transition metal. Claimed invention demonstrates antifungal activity with respect to causative agents of mycoses from the group, including Trichophyton mentagrophytes var. Interdigitale, Trichophyton rubrum, Microsporum canis, Trichophyton mentagrophytes var. granulosum, Candida albicans. Antifungal activity has been detected with respect to pathogenic and opportunistic fungi, causing mycoses of mucosa and skin in humans and animals.

EFFECT: increased activity of preparation.

9 cl, 4 tbl, 28 ex

FIELD: medicine, pharmaceutics.

SUBSTANCE: invention refers to medicine, particularly to a method for ferrocene encapsulation characterised by the fact that a microcapsule coating is carrageenan, whereas a non-solvent is ethanol when producing microcapsules by physical-chemical non-solvent addition.

EFFECT: implementing the invention enables simplifying and accelerating the process of encapsulation and increasing weight yield.

7 dwg

FIELD: medicine.

SUBSTANCE: invention refers to medicine, namely to haematology and enterology, and concerns treating anaemia in the patients with celiacia. That is ensured by an oral administration of iron bis-glycinate chelate in the form of an effervescent tablet, semi-solid or liquid dosage form in the range of doses of 5 to 200 mg a day that corresponds to 1 to 40 mg of an iron ion.

EFFECT: invention provides treating anaemia in the patients suffering celiacia by the effective absorption of orally administered iron bis-glycinate chelate.

11 cl, 3 tbl, 11 ex

FIELD: medicine, pharmaceutics.

SUBSTANCE: invention refers to veterinary science and aims at treating and preventing alimentary anaemia in young pigs. A preparation contains an iron dextran complex, nanosized and zero-valent selenium (Se0), vitamin E, vitamin B12 and water in the following proportions, wt %: iron dextran complex 0.0001-80.0, selenium (Se0) 0.0001-5.0, vitamin E 0.0001-20.0, vitamin B12 0.0001-10.0, water for injections - the rest.

EFFECT: using the declared method provides compensating iron deficiency in young pigs, improving animal's growth and development, increasing total immunity and rapid adaptation to the varying ambient environment.

4 tbl, 4 ex, 4 dwg

FIELD: veterinary medicine.

SUBSTANCE: method of normalisation of the thrombin time duration in newborn calves with iron deficiency anemia consists in the fact that ferroglukin 150 mg (2 ml) intramuscularly is prescribed to newborn calves with iron deficiency anemia, twice with the interval of 4 days, cresacyne 5 mg/kg per day, including it in the scheme of watering for 4 days, starting simultaneously with the first injection of ferroglukin and gamavit intramuscularly once a day in the morning of 0.05 ml/kg for 4 days, starting simultaneously with ferroglukin and cresacyne.

EFFECT: acceleration of normalisation of thrombin time duration, enables to reduce the risk of vascular complications in newborn calves with iron deficiency anemia, to revitalise the herd, to reduce mortality, to maintain the volume and quality of the meat and dairy products obtained from animals.

2 ex

FIELD: medicine, pharmaceutics.

SUBSTANCE: invention refers to chemical-pharmaceutical industry and represents a method for drug encapsulation by non-solvent addition; the method is characterised by the fact that the above drug is presented by ferrocene, while a coating is carrageenan precipitated from a butanol solution by adding ethanol as a non-solvent and water at room temperature.

EFFECT: invention provides simplifying and accelerating the microencapsulation process, reducing the microencapsulation loss (higher weight yield).

2 ex, 2 dwg

FIELD: medicine.

SUBSTANCE: invention refers to veterinary science, namely to clinical pharmacology and veterinary therapy. The method consists in administering the complex iron-dextran preparation Ferranimal-75M intramuscularly on the 5th day of calf's life in a dose of 3 ml in a combination with an intramuscular injection of the preparation Hydropeptone in a dose of 10 ml. Ferranimal-75M is injected 10 days later in a dose of 2 ml in a combination with an injection of Hyropeptone 5 ml intramuscularly in different points.

EFFECT: method provides higher antioxidative activity of calf's blood serum, reduced pro-oxidant action of iron and incorporated radionuclides, as well as higher iron accessibility in treating and preventing iron-deficiency anaemia in calves exposed to the chronic incorporated radiation.

FIELD: veterinary medicine.

SUBSTANCE: ferroglyukin is administered to new-born calves with iron deficiency anaemia at a dose of 150 mg (2 ml) intramuscularly, twice with an interval of 4 days. Crezacyne 5 mg/kg per day is included in the watering scheme for 4 days, starting simultaneously with the first injection of ferroglyukin. Gamavit is administered intramuscularly once a day in the morning at a dose of 0.05 ml/kg for 4 days, starting simultaneously with ferroglyukin and crezacyne.

EFFECT: method enables to normalise consistently the platelet activity in new-born calves with iron deficiency anaemia during a short period of exposure, transferring it to the level typical of healthy calves, after 4 days of treatment, and to provide long-term maintenance of platelet haemostasis in the optimal mode of operation, eliminating the risk of thrombotic complications in animals and contributing to their normal growth and development.

1 ex

FIELD: medicine, pharmaceutics.

SUBSTANCE: invention relates to veterinary and can be applied in pig-breeding for the stimulation of exchange processes, growth activity, prevention of hypomicroelementosis and diarrhoeas of piglets. An energometabolic composition for the stimulation of exchange processes represents a water solution, which contains succinic acid, iodinol, beet molasses and sulphates of iron, copper, cobalt, zinc, with the following component content in 1000 ml of the solution: succinic acid - 10.0 g, iodinol - 250 ml, beet molasses - 300 ml, iron sulphate - 2 g, copper sulphate - 0.1 g, cobalt sulphate - 1 g, zinc sulphate - 0.3 g, water- the remaining part.

EFFECT: application of the invention will make it possible to improve exchange processes in piglets.

4 tbl

Up!