Agent for treatment of enteritis in animals

FIELD: veterinary medicine.

SUBSTANCE: agent comprises enrofloxacin and excipients. The agent additionally comprises humates of peat or sapropel, with the following ratio of the components, wt %: enrofloxacin substance - 0.5; alkaline solution of humates (pH=12-13) - 80; succinic acid - to pH 10-11; propylene glycol - the rest. The agent is administered with the liquid feed once a day for three to five days at a dose of from 0.5 to 1.0 ml per 1 kg of body weight.

EFFECT: manifestation of pronounced therapeutic effect.

5 tbl, 2 ex

 

The invention relates to agriculture, in particular to veterinary medicine and can be used in animal husbandry as feed additive for treatment of digestive disorders in animals and birds.

Known antibacterial agent from the group of fluoroquinolones is enrofloxacin used to treat Pets with bacterial infections, for internal (through the mouth), and injection applications.

A disadvantage of the known device is the lack of non-specific stimulant (tonic) of the action of the animal organism and the presence of negative action, which is manifested in the disruption of cartilage in young animals. When using enrofloxacin strictly prohibited concomitant use of nonsteroidal anti-inflammatory drugs, macrolides and tetracyclines. Contraindication to the use of the drug is also the detection of pathogenic strains of bacteria that are resistant to enrofloxacin.

Closest to the claimed remedy for the treatment of bacterial enteritis (colibacillosis, salmonellosis, necrotic enteritis and other) animals are drugs for oral (internal) dose on the active substance 2.5-5 mg/kg, 1 time a day, 3-5 days: "Enroflon®-10% solution for oral use, containing in 1 ml 100 mg Enraf�of oxazine and auxiliary substances: potassium hydroxide, benzyl alcohol, water, manufacturer: LLC "VIC - animal health", Russia, registration number PVR-2-2.9/00089 (cited: Medicinal products for veterinary use in Russia. Directory "Vidal expert". - M.: Attraversare. - 2013. - P. 360-361); "Enroflox in 1 ml 100 mg enrofloxacin and auxiliary substances: potassium hydroxide, benzyl alcohol, purified water, manufacturer INVESA, Spain, registration number PVI-2-3.5/00438 from 24.02.11 (cited: Medicines veterinary use in Russia. Directory "Vidal vet". - M.: Attraversare. - 2011. Pp. 419-420). All of these analogues are summarized by the similarity in that their solvents have high alkalinity (pH=9,5-11,5). In other sources - "Injection or infusion solution of enrofloxacin" (patent RF №214722, filing: 28.12.1995) and "Treatment of mastitis by using enrofloxacin (patent RF №2413514, applying 28.10.2005), which is predominantly acidic form of complex solvents.

It is known tool for the prevention of animal diseases by raising nonspecific resistance and accelerating growth under the General title "humates", derived from natural organic raw materials (coal, peat, sapropel) by alkaline hydrolysis, is used as a feed additive, non-antibiotic properties.

Blueblack to the claimed means is "Guvitane" - liquid product of alkaline hydrolysis of peat containing humic substances used in animal feed additives to enhance their disease resistance by enhancing the processes of digestion, normalizes metabolism, binding of toxins in the gastro-intestinal tract (TU 9283-00256462103-2010. Manufacturer LLC "Russ", Voronezh, 394088, 97). A method of producing and applying humanidades product (alkali hydrolysate of sapropel) in anemia of calves in combination with transcranial electrical stimulation (Russian Federation No. 2392945, application: 2008130210/13, 21.07.2008).

The lack of Guvitane and alkaline hydrolysate of sapropel is the complete absence or the presence of weak antibacterial action on pathogens, causing enteritis.

However, each of the above agents used for animals separately, and was not determined by their joint role in the treatment of animals with bacterial enteritis.

The aim of the invention is to provide a novel means for the treatment of bacterial enteritis in animals having anti-microbial and non-specific stimulating effect and for reducing death, disease severity, and the duration of treatment (not less than 10%).

The technical result is used to ensure the improvement of results of treatment of animals with enteritis, exp�jaydees to reduce the duration and severity of the disease, reducing the number of relapses and the value of the damage avoided from disposal of animals.

The technical result is achieved by the combined expression of mutually reinforced therapeutic properties of the components and mutual funds eliminate the disadvantages of each component, which is impossible by the separate application of each component.

The distinctive features are that to achieve better therapeutic action of internal (oral) use with liquid feed enrofloxacin enteritis in animals it is dissolved in an alkaline solution of humates.

The essence of the proposed tools is that making money is by obtaining a solution of the antibacterial component of enrofloxacin (from 0.2 to 1.0 wt.% in an alkaline solution of humic substances (from 0.1 to 0.5 wt.%) sapropel (peat). Preferred alkaline solutions due to the fact that the enteritis recorded mainly in young animals (except enterotoxaemia piglets) in the diet which contain easily fermentable to lactate (lactic acid) lactose, manifested in the form of "acid catarrh of the bowel". For optimal pH is applied to the base (NaOH, KOH) and acid (succinic acid), to prevent crystallization of the solution used propylene glycol (1,2-propandiol). Cu�IU, succinic acid in the form of salts (succinates) and propylene glycol (dihydric alcohol) provide buffering environment in the gastro-intestinal tract, and imbibing, contribute to the normalization of energy metabolism, participating in the cycle (metabolism) tricarboxylic acids, thus preventing the development of metabolic acidosis. The combination of these properties offer the means provides increased therapeutic efficacy as a result of simultaneous complex influence of the components means on the patient's body of the animal and of the disease, manifested in the acceleration of the recovery of animal health and reducing the possible side effects of enrofloxacin. This reduces production costs: instead of producing two separate alkaline solutions of enrofloxacin and humates offers a simplified technology of obtaining one means by dissolving the powder substance enrofloxacin in an alkaline solution (pH=12-13) of humates with subsequent introduction into the solution of succinic acid to obtain pH=10-11 and adding propylene glycol to the masses.% 10-30.

Examples of obtaining funds for the treatment of bacterial enteritis in animals:

Table 1
P�emer 1
The name of the componentLimitsCompounding tools
AndEnrofloxacin substance, wt.%0.2 to 1.00,5
BA solution of humates, wt.%70-9080
- humic substances, wt.%0,1-0,50,3
- sodium hydroxide (or potassium hydroxide)to pH=12-13
is succinic acidbefore reaching pH=10-11
InPropylene glycol, wt.%10-30else

Table 2
Example 2
The name of the componentLimits Compounding tools
AndEnrofloxacin substance, wt.%0.2 to 1.00,5
B"Guvitane" TU 9283-00256462103-2010.70-9080
InSodium hydroxide (or potassium hydroxide)to pH=12-13
GSuccinic acidbefore reaching pH=10-11
DPropylene glycol, wt.%10-30else

Table 3
Example 3
The name of the componentLimitsCompounding tools
AndEnrofloxacin substance, wt.%0.2 to 1.00,5
B.�CNY hydrolysate of sapropel with pH=12-13, wt.% (patent RF №2392945)70-9080
InSuccinic acidbefore reaching pH=10-11
GPropylene glycol, wt.%10-30else

Clinical studies:

Example 1

Experience the study of therapeutic effectiveness of the proposed tools in comparison with the known "Enroflon®-10% solution for oral administration" and "Guvitane" was performed on the calves with a diagnosis of acute indigestion, bacterial (bacterial enteritis etiology) aged from 2-3 days up to 1 month at the dairy farms of the Omsk and Tyumen regions. In the experimental group means were given to calves individually inside mixed with milk (colostrum) when feeding once a day at a dose of 1.0 ml per 1 kg of body weight, with a daily dose of the active substance enrofloxacin was 5 mg/kg of body weight of calves. In the 1st control group calves was used for treatment "Enroflon" once a day with liquid feed at a daily dose of 5 mg/kg of body weight. In the 2nd control group "Guvitane" calves injected with liquid feed at 0.8 ml per kg of body weight of 1 times a day.

Analysis of the test results (table 4) St�indicates the best therapeutic efficacy of the developed product. So, in the experience received 100% of therapeutic effect of the use of the proposed funds, and in the control groups, which were respectively applied "Enroflon" and Guvitane", the efficiency was lower and amounted to, respectively, 97% and 56%. In addition, in the experimental group recovery (on the basis of disappearance of the main symptom of the disease - diarrhea) occurred earlier at 34%, and there was no death of animals and the recurrence of the disease. The data obtained indicate that the proposed facility on medical action is superior to "Enroflon" and "Guvitane".

18
Table 4
Comparative effectiveness of treatment for bacterial enteritis calves proposed facility in comparison with the Enroflon" and "Guvitane"
Indicators of efficiency of treatment of calvesGroups of animals
ExperienceControl 1Control 2
Tool"Enroflon""Guvitane"
The number of animals in the group, goals4734
The amount of consumed doses of disinfectants tested on 1 calf and / total per group5 (235)5 (170)5 (90)
Came the disappearance of the main symptom of the disease (diarrhea) on average, 1 calf, days2,533,41-
Came the disappearance of the main symptom of the disease (diarrhea) in calves, heads / %:
on the second day, goals / (%)21 /459/270
- on the third day (cumulative number), heads / %39/8317/505/27
- on the fourth day, goals / (%)45/9628/8212/67
- on the fifth day, goals / (%)47/10034/10015/83
Left sick calves, a goal. / %00 3/17
The number of dead animals, heads / %001/6
The number of relapses of the disease, cases / %014/22
Therapeutic efficacy, %1009756

Example 2

Second experience-finding the effectiveness of preventive therapy (drug prevention) the proposed remedy for mass diseases of newborn calves was carried out in comparison with the two drugs, Enroflon" and Guvitane", as well as for General control were taken as statistical data on morbidity and mortality of calves that preventive therapy was not performed, and the causes of disease were similar in all groups: high bacterial contamination of animal pathogenic bacteria of group of an intestinal stick. Subjects funds asked the calves inside with liquid food (milk, colostrum) once daily in the morning feeding for 5 days, starting from the first feeding, doses, calculated as ml/kg body weight: medium - 1; Enroflon - 0.05 and Guvitane and 0.8.

The effectiveness of preventive therapy (table 5) in about�ITE and in two control groups were, respectively, 96%, 93% and 35%, and the ratio of reduction in the incidence of animals covered by groups respectively 11, 6 and 2 times. The results obtained indicate that the application of the proposed tools has a strong preventive action against bacterial enteritis calves and efficiency superior to the "Enroflon" and Guvitane".

Table 5
Preventive therapy (drug prevention) at a mass of bacterial enteritis in neonatal calves (with an incidence of campylobacteriosis in the first 10 days of life - 45% of the number of births)
Performance indicators preventive therapy calvesGroups of animals
ExperienceControl 1Control 2Control total
Tool"Enroflon""Guvitane"Without preventive therapy
The number of animals in the group, goals1205731 60
The amount of consumed doses of disinfectants tested on 1 calf and/ total per group5 (600)5 (285)5 (155)0
Sick calves for 10 days, a goal.54627
The incidence of calves in the first 10 days of life, goals for 100 calves471945
Palo calves, goal0014
The multiplicity of reducing the incidence, time1162-
Preventive effect on 100 calves, a goal.9693350

Analysis of the results of clinical trials proposed remedies for the treatment of pigs, chickens and rabbits, patients with bacterial enteritis, the�showed good therapeutic efficacy. On animals other types of testing not conducted.

Thus, the application of the proposed remedies for the treatment of bacterial enteritis in young farm animals (calves, pigs, chickens, rabbits) has a more pronounced therapeutic effect in comparison with the prototype "Enroflon" and Guvitane".

The tool is simple to use and effective. Its production does not require large expenditures.

Agent for the treatment of bacterial enteritis in animals, for with liquid feed once a day for three to five days at a dose of 0.5 to 1.0 ml per 1 kg of body weight, containing enrofloxacin and excipients, characterized in that it further contains humates from peat or sapropel in the following ratio of components, wt.%: enrofloxacin substance is 0.5; the alkaline solution of humates (pH=12-13) - 80; succinic acid to pH 10-11; propylene glycol - the rest.



 

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