Method of extracting novocain from water solution

FIELD: chemistry.

SUBSTANCE: method of Novocain extraction from water solutions includes the preparation of an aqueous salt solution of Novocain by its dissolution in a saturated solution of a salting-out agent, extraction and analysis of an equilibrium water phase, with the application as an extragent of a solvotrophic reagent solution in chloroform with the concentration of 10 wt %, for which purpose the aqueous salt solution of Novocain with pH 8.0±0.5 is prepared due to the application of a saturated ammonium sulphate solution as the salting-out agent and addition of an ammonium buffer solution, Novocain is extracted for 5-7 minutes with the solution of the solvotrophic reagent in chloroform with the ratio of volumes of the aqueous salt solution of Novocain and extragent of 5:1, then the aqueous saline phase is separated from the organic one and analysed by a method of UV-spectrophotometry at a wavelength of 291 nm, the concentration of Novocain in the water solution is found by means of a calibrating graph; the coefficient of distribution (D) and the degree of extraction (R, %) of Novocain is are calculated by formulae.

EFFECT: method for the extraction of Novocain from the water solution, which is characterised by expressiveness, makes it possible to realise the practically complete single extraction of Novocain from the aqueous salt solution and can be applied in the analysis of Novocain-containing water solutions.

1 ex

 

The invention relates to analytical and pharmaceutical chemistry and can be used for the extraction of novocaine from aqueous media with the purpose of its further determination.

The closest in technical essence and the achieved effect is a method of extraction of novocaine from aqueous media with the use of chloroform without adding vicariates [Korenman Ya. I. distribution Coefficients of organic compounds. Guide. Voronezh: Publishing house of Voronezh state University. 1992. 336 p.]. The disadvantage of this method is the low distribution coefficient of novocaine (27,5) in the specified system.

The technical problem of the invention is to develop extraction systems with the aim of achieving a high coefficient of novocaine and almost complete extraction from aqueous solutions of chloroform with the use of allotropes reagent.

The technical result of the invention is to achieve a high distribution ratio of novocaine (up to 229,2), almost complete (98%) extract of novocaine from the aqueous solution with a solution of camphor in chloroform in the presence of ammonium sulfate.

Said technical result is achieved in that the extraction of novocaine from aqueous media involves the preparation of a solution of novocaine by dissolving in a saturated solution of vicariates, extraction and Ana�from the equilibrium water phase, the extractant solution using allotropes reagent in chloroform with a concentration of 10 wt. % why prepare a water-salt solution of novocaine with application as vicariates saturated solution of ammonium sulfate and add ammonia buffer solution, extracted with novocaine for 5-7 min with a solution allotropes reagent in chloroform in a volume ratio of water-salt solution of novocaine and extractant 5:1, separating the water-salt phase from the organic and analysed by UV-spectrophotometry at a wavelength of 291 nm, calibration chart to find the concentration of procaine in aqueous solution.

The implementation of the method is illustrated by the following example. Prepare a water-salt solution of novocaine, which has a pH of 8.0±0,5 due to the use as vicariates saturated solution of ammonium sulfate and add ammonia buffer solution, for which a portion of novocaine pharmacopoeial purity, taken on an analytical balance, placed in a chemical beaker 50 cm3was dissolved in a small volume (2-3 cm3) of distilled water, after complete dissolution of the sample (30-35 h) the solution was transferred to volumetric flask of 100 cm3, bring to mark with a saturated solution of ammonium sulfate, extracted with novocaine solution of camphor in chloroform, for this � received novocaine solution was added a solution of camphor in chloroform with a concentration of 10 wt. % when the volume ratio of water-salt solution of novocaine and extractant 5:1, is extracted on vibromaster before the establishment of the interfacial equilibrium (5-7 min). After stratifying system of water-salt phase is separated from the organic and analysed by UV-spectrophotometry. Measure the optical density of a water-salt solution on a UV spectrophotometer in a quartz cell with a thickness of the light-absorbing layer of 1 cm at a wavelength of 291 nm, build a calibration graph of optical density of water-salt solution from the concentration of novocaine, on schedule find the concentration of procaine in aqueous solution.

The distribution coefficient (D) and the degree of extraction of novocaine after extraction (R, %) is calculated according to the formulas:

,,

where Caboutand Cin- concentration of novocaine in the organic and aqueous phases, µg/cm3; f - the ratio of the equilibrium volumes of aqueous and organic phases.

The organic phase is transferred 98% of novocaine from its original content in the analyzed solution, the distribution coefficient equal 229,2.

As you can see, the high distribution coefficient (229,2) and almost complete (98%) removal of novocaine solution of camphor in chloroform, when the volume ratio of water-salt solution and EXT�agent of 5:1 and use as vicariates of ammonium sulfate. The analysis duration 30-35 minutes.

When used as vysalivatel sodium chloride and lithium sulfate the degree of extraction of novocaine does not exceed 50% when the volume ratio of aqueous and organic phase is 10:1 and 15:1 the degree of recovery of less than 40%.

The proposed method of extraction of novocaine from aqueous media allows you to reach high values of distribution coefficient (up to 229,2) and almost complete (98%) extract of novocaine from water-salt solution, characterized by rapidity (analysis time 30-35 minutes) and can be applied in the analysis of aqueous media containing novocaine.

Method of extraction of novocaine from aqueous solutions, comprising preparing a water-salt solution of novocaine by dissolving in a saturated solution of vicariates, extraction and analysis of the equilibrium aqueous phase, characterized in that the extractant solution using allotropes reagent in chloroform with a concentration of 10 wt.%, why prepare a water-salt solution of novocaine with pH 8,0±0,5 due to the use as vicariates saturated solution of ammonium sulfate and add ammonia buffer solution, extracted with novocaine for 5-7 min with a solution allotropes reagent in chloroform in a volume ratio of water-salt solution of novocaine and extractant 5:1, further oteleuton-salt organic phase and analyzed by UV-spectrophotometry at a wavelength of 291 nm, on the calibration chart to find the concentration of procaine in aqueous solution; calculate the distribution coefficient (D) and the degree of extraction (R, %) of novocaine by the formulas:
,
where Caboutand Cin- concentration of novocaine in the organic and aqueous phase after extraction, respectively, mg/cm3; f - the ratio of the equilibrium volumes of aqueous and organic phases.



 

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