Method for predicting risk of hysteromyoma
SUBSTANCE: invention represents a method for predicting a risk of isolated hysteromyoma involving sampling and studying peripheral venous blood, differing by the fact that DNA is recovered from the peripheral venous blood; genetic polymorphisms of interleukin genes are typed, and combinations of polymorphisms of interleukin-1 (-889 TT IL-1) and interleukin-5 (-703 C IL-5) genes are analysed; a high risk of isolated hysteromyoma is predicted, if observing a combination of -889 TT IL-1 allele and -703 C IL-5 allele in Russian females, native of the Central Black Earth Region.
EFFECT: invention provides obtaining criteria of the risk of isolated hysteromyoma as shown by the combination of the molecular-genetic markers 889 TT IL-1A and -703 C IL-5.
1 ex, 1 tbl, 2 dwg
The invention relates to the field of medical diagnostics, namely to gynecology, and can be used to predict the risk of formation of isolated uterine fibroids.
The problem of uterine fibroids in women of all age periods is one of the important in modern gynecology. This is because uterine fibroids - the most common benign tumor of the female genital organs, not only in the perimenopausal period, when it is found in more than 50% of women, but in their reproductive years. Most often uterine fibroids diagnosed in women aged 35 years, at the age of 35-55 years, accounts for 90% of the total number of diseases, and rarely uterine fibroids common in women younger than 20 and older than 70 years. Uterine fibroids cause menstrual disorders, anemia, chronic pelvic pain. From 60 to 95% of all surgical interventions in women of reproductive age with uterine cancer accounts for radical surgery, leading to loss of reproductive and menstrual function and expressed by vascular and psycho-emotional disorders. Early detection of uterine fibroids will allow for a more efficient conservative treatment and thereby reduce the risk of surgical intervention [Sidorova I. S. uterine Fibroids (modern problems of etiology, pathogenesis, diagnosis, and treatment). - M., 2003].
A special role in the mechanisms of tumor growth is given to the processes of apoptosis. Tumor growth is the sum of the dynamic balance between proliferation and cell death. Thus, growth can be associated with excessive proliferation and low index of cell death. Recently, data were obtained that the cell is able to receive signals how to divide, and to death, triggering a cascade of complex processes leading to fragmentation of the cell with the formation of the membrane surrounded the so-called apoptotic cells. This process is called apoptosis, i.e. programmed cell death. Important in the mechanisms of apoptosis is given to interleukins. Currently identified more than two dozen interleukins (interleukin-1, interleukin-2, etc.). They regulate intercellular and inter-system interactions that determine cell survival, stimulation or inhibition of their growth, differentiation, functional activity and apoptosis, and ensure the consistency of the action of the immune, endocrine and nervous system at the cellular level in normal conditions and in response to pathological effects [Golubeva O. V. Genetic factors predisposing to the development of adenomyosis / O. V. Golubeva, T. E. Ivashchenko, V. S. Baranov, E. K. aylamazyan // Journal of obstetrics and female diseases. 2007. - T. 1. - No. 2. - P. 2-38].
In the patent of Russian Federation №2355319, publ. 20.08.2012 on application No. 2007137713/14 from 11.10.2007 (Kulagina N. In., Kozlov V. V.) proposed a method of predicting the course of uterine fibroids with the development of clinical symptoms, including the determination of the age of menarche, the availability of medical abortions before the first birth, the presence of inflammatory complications of medical abortion, the presence of hypertension during the previous pregnancies, the presence of anomalies of labor activity, body mass index, presence of hypertension and presence of chronic diseases of the gastrointestinal tract and the calculation of the indicators predict the clinical course of uterine fibroids. On the basis of its value forecast for uterine fibroids with the development of clinical symptoms. The method does not allow to predict the formation of isolated uterine fibroids.
A prototype of the selected RF patent №2340897, publ. 10.12.2008 by application No. 2007126877/15 dated 16.07.2007 "a Method of predicting rapid growth of uterine leiomyoma" (Sotnikova N. Yu. (RU), A. Sosnina E. (RU), Boyko O. M. (RU)), where a method of predicting rapid growth of leiomyoma of the uterus, including the fence and the study of peripheral venous blood, determination in peripheral blood of women with uterine leiomyoma relative amount of IL-8+ monocytes and in the case that the values of this index equal to or greater than 63%, the forecast quickly�on the growth of leiomyoma during the year.
The disadvantage of the prototype is that the proposed method allows to predict the growth of uterine leiomyoma, but makes it impossible to predict the formation of isolated uterine fibroids.
The object of the present invention is to provide a method for predicting the formation of an isolated form of uterine fibroids according to combinations of genetic polymorphisms: -889 TT IL-1 together with -703 With IL-5.
The technical result of the invention - preparation of risk assessment criteria for the formation of isolated uterine fibroids in women of Russian nationality, which urozhencami of the Central Chernozem region of Russia.
In accordance with the assigned task has been developed a method of predicting the formation of isolated uterine fibroids, including:
the fence and the study of peripheral venous blood;
- extraction of DNA from peripheral venous blood;
- typing of genetic polymorphisms of interleukin (-889 C/T IL-1 and -703 C/T IL-5);
- analysis of combinations of gene polymorphisms of interleukin-1 (-889 TT IL-1) and interleukin-5 (-703 With IL-5);
- prediction of high risk of development of isolated uterine fibroids in patients in case of detection of allele combinations -889 TT IL-1 and allele -703 With IL-5.
Novelty and inventive step lies in the fact that the prior art is unknown to the ability of the forecast time�itia isolated uterine fibroids by the presence of allele combinations -889 CT of the gene of interleukin-1 and allele -703 With interleukin-5 in women of Russian nationality, which urozhencami of the Central Chernozem region of Russia.
The invention is characterized by the following graphical image.
Fig. 1. Electrophoretic separation of the products of restriction gene -703 C/T IL-5., where 1, 3-8, 12, 15 homozygotes -703 MOP; 10, 11, 13, 14 heterozygotes -703 ST; 2, 9 homozygotes -703 TT.
Fig. 2. Electrophoretic separation of the products of restriction gene -889 C/T IL 1A., where 2, 3, 4 homozygotes -889 CT; 5, 6, 10 - homozygote -889 SS, 1, 7-9, 11-14 heterozygotes -889 CT.
The method is as follows.
DNA extracted from samples of peripheral venous blood of patients with proliferative diseases of the uterus (uterine fibroids with endometriosis, endometrial hyperplasia) by the method of phenol-chloroform extraction in 2 stages. In the first stage to 4 ml of blood add 25 ml of lyse buffer containing 320 mm sucrose, 1% Triton X-100, 5 mm MgCl210 mm Tris-HCl (pH 7.6). The resulting mixture was stirred and centrifuged at 4°C, 4000 rpm for 20 minutes. After centrifugation the supernatant decanted, to the residue add 4 ml of a solution containing 25 mm EDTA (pH 8.0) and 75 mm NaCl, resuspension. Then add 0.4 ml 10% SDS, 35 ál proteinase K (10 mg/ml) and the sample was incubated at 37°C for 16 hours.
In the second phase from the resulting lysate sequentially perform the extraction of DNA equal volumes of phenol, phenol-chloroform (1:1) � of chloroform by centrifugation at 4000 rpm for 10 minutes. After each centrifugation produce a selection of the aqueous phase. DNA is precipitated from the solution with two volumes of chilled 96% ethanol. Formed DNA is dissolved in twice-distilled, deionized water and stored at -200°C.
The selected DNA is then subjected to polymerase chain reaction using standard oligonucleotide primers (table 1).
Molecular genetic analysis is carried out by polymerase chain reaction of DNA synthesis. PCR is performed on the amplifier TP-PCR-01-TERZIC". Analysis of polymorphism of genes -889 C/T IL-1A, -703 C/T IL-5 is carried out by polymerase chain reaction of DNA synthesis using standard oligonucleotide primers by the method, described in reference [Hulkkonen, J. Inflammotory cytokines and cytokine gene polymorphisms in chronic lymphocytic leukaemia, in primary Sjögren''s Syndrome and Haelthy Subjects: [acad. diss.] / J. Hulkkonen; University of Tampere, Medical School Tampere University Hospital. - Tampere, 2002. - 81 p.].
The reaction is carried out in a 12.5 µl total volume of the mixture containing 33.5 mm Tris-Hcl (pH 8,8), 1.25 mm MgCl2, 0.5 μg of genomic DNA, 5 PM of each primer, 100 µm dATP, dGTP, dCTP, dTTP, and 1 unit of active Taq polymerase. After denaturation perform a 32 cycle amplification scheme: denaturation, primer annealing and elongation. Then, the samples incubated for 5 min at 72°C and cooled. The amplification products analyzed in 2% agarose gel, okrasa�nom with ethidium bromide, for 30 minutes at 160 V. as electrophoretic buffer used 1×TAE (Tris-acetate buffer). Then, the samples identified in transmitted UV light.
Genotyping of DNA markers (loci -889 C/T IL-1A, -703 C/T IL-5) produced by the method of analysis of the polymorphism of the lengths of restriction fragments (RFLP) of PCR products amplification of specific genomic regions using appropriate enzymes produced by "Simanim" (Novosibirsk).
After incubation, the restriction mixture for 16 hours at 37°C carry out the separation of DNA fragments by horizontal electrophoresis in an electrophoretic cell production company "Helikon" (Russia). Depending on the size of the partial DNA fragments using agarose gel 2-3% concentration, prepared on the basis of TBE buffer, stained with ethidium bromide solution (0,01%). Results visualization of trigram carried out in a dark box with a transilluminator company UVP (Sweden), shown in Fig. 1 and Fig. 2.
Creation of database and statistical calculations were performed using the program STATISTICA 6.0 [Borovikov, V. Statistica: the art of computer data analysis / V. Borovikov. - 2nd ed. - SPb.: Peter, 2003. - 688 p.: ill. - (For professionals)].
Study of the role of combinations of genetic variants of polymorphic markers �enow interleukins in the formation of isolated uterine fibroids was performed using the software APSampler [http:sources.redhat.com/cygwin/], using Monte-Carlo Markov chains and Biesbosch nonparametric statistics.
Assessment of the level of statistical significance of the obtained results was performed using the Bonferroni (pcor), i.e. amendments that minimizes the probability of false positive results [Rebrova O. Yu., Statistical analysis of medical data. The use of the Statistica / O. Y. Rebrova // M: media sphere. - 2006 - 312 p.].
The possibility of using the proposed method for assessing increased risk of isolated uterine fibroids confirms the results of the observations 713 patients with proliferative processes of the reproductive system, as well as 500 people population control. Among surveyed 713 women isolated uterine cancer was diagnosed in 117 patients. Patients were included in the relevant group of patients only after diagnosis of the disease, confirmed by clinical, laboratory and instrumental methods of examination and confirmed by histology data.
In the current study included individuals of Russian nationality who are nationals of the Central Chernozem region of Russia and have no relationship among themselves.
It is established that the combination of two genetic variants -889 TT IL-1A and -703 With IL-5 in the patient group�to with isolated uterine cancer occurs (14,47%) significantly more often (in 6.8 times), than in the control group (2,12%, p=0.0001, pcor=0,02). These data suggest a significant contribution of combinations of polymorphic variants of genes -889 C/T IL-1A and -703 C/T IL-5 in the formation of an isolated form of uterine fibroids (OR=7,78, 95% CI 2,61-23,21).
A specific example of the proposed method
Patient X Russian nationality, a native of the Central Chernozem region of Russia, were held the fence and the study of peripheral venous blood, extraction of DNA from peripheral venous blood; the typing of genetic polymorphisms of interleukins -889 C/T IL-1 and -703 C/T IL-5 and analysis of combinations of polymorphisms -889 TT IL-1 and -703 With IL-5. The results are presented in Fig. 1 and Fig. 2.
It is revealed that due to the presence of a combination of molecular genetic markers -889 allele TT IL-1A and allele -703 With IL-5 woman can be attributed to the risk group of the formation of isolated uterine fibroids and pick up individual tactics to conservative therapy and reduce the risk of surgical intervention.
Thus, the proposed method solves the problem and ensures the achievement of the technical result - obtaining criteria for the assessment of risk of formation of isolated uterine fibroids on a combination of molecular genetic markers -889 TT IL-1A and -703 With IL-5 (OR=7,78).
The use of this method will allow you to identify the risk group R�svitil isolated uterine fibroids in patients of Russian nationality, of female of the Central Chernozem region of Russia, and to pick up individual tactics to take remedial measures to conduct conservative treatment and reduce the risk of surgical intervention.
A method of predicting risk of development of isolated uterine fibroids, including the fence and the study of peripheral venous blood, characterized in that from the peripheral venous blood DNA isolated, carried out the typing of genetic polymorphisms of interleukins and analysis of combinations of gene polymorphisms of interleukin-1 (-889 TT IL-1) and interleukin-5 (-703 With IL-5), predict a high risk of isolated uterine fibroids in case of detection of allele combinations -889 TT IL-1 and allele -703 With IL-5 in women of Russian nationality, of female the Central Chernozem region of Russia.
SUBSTANCE: method includes the preparation of smear from peripheral blood with preliminary fixation with methyl alcohol, drying, washing with distilled water. After that, the smears are placed in a potassium chloride solution in a ratio of 0.57 g of potassium chloride per 100 ml of distilled water for 20 min and washed with distilled water. Additionally prepared is a mixture of solutions, prepared ex tempore, containing a solution "A" and "B". The solution "A" includes a 50% silver nitrate solution in an amount of 5 g of silver nitrate + 5 ml of distilled water. The solution "B" includes a 2% solution of gelatin on a 1% formic acid solution in an amount of 15.8 ml of distilled water + 0.2 ml of 100% formic acid + 4.0 ml of 10% gelatin. The solutions "A" and "B" are mixed in an amount of 5 ml of each, in darkness, with further submergence of the blood smears for 20 min in darkness in the thermostat at a temperature of 37°C with the further submergence of the smears into distilled water for 2-3 seconds. After that, they are twice subjected to a 8 min exposure in a 5% sodium thiosulphate solution in darkness in the thermostat at a temperature of 37°C. After that, they are washed successively with tap water and distilled water, after-staining is performed in the Romanovskiy dye for 30 min. After that, the smears are washed again with tap water, air-dried, placed in the Canadian balm and covered with a coverslip.
EFFECT: increased quality of smear staining and provision of a possibility to identify and further evaluate parameters of nucleolus organiser regions.
4 tbl, 6 ex
SUBSTANCE: patients are assessed individually to establish the fact of chronic cardiac insufficiency (CCI) suffered by detecting: age, height, weight, Quetelet index, Brock constant, systolic blood pressure, diastolic blood pressure, triglycerides, total cholesterol, high-density lipoprotein cholesterol, low-density lipoprotein cholesterol, low-density lipoprotein cholesterol to total cholesterol ratio, very low-density lipoprotein cholesterol, high-density lipoprotein cholesterol to low-density lipoprotein cholesterol ratio, sum of the very low-density lipoprotein cholesterol and high-density lipoprotein cholesterol to low-density lipoprotein cholesterol ratio, atherogenic index, alanine aminotransferase, aspartate aminotransferase, aspartate aminotransferase to alanine aminotransferase ratio, lactate dehydrogenase, glucose, α-amylase, total protein, albumin, uric acid, carbamide, creatinine, creatinine kinase, alkaline phosphatase, total bilirubin, conjugated bilirubin, ADP-induced thrombocyte aggregation, thrombocyte count, mean thrombocyte volume, erythrocyte count, mean erythrocyte volume, erythrocyte volume distribution, haematocrit, haemoglobin, mean corpuscular haemoglobin, mean corpuscular haemoglobin concentration, leukocyte count, segmented neutrophil percentage, eosinophil percentage, basophile percentage, lymphocyte percentage, monocyte percentage and displacement constant. The derived discriminator functions are used to establish the fact of the absence of CCI, the first stage of CCI, or the second stage of CCI.
EFFECT: method enables assessing the patients to establish the fact of CCI by determining the significant parameters.
3 dwg, 2 tbl, 1 ex
SUBSTANCE: method of determining sensitivity of fluorescence spectra comprises cultivation of P. aeruginosa strains on nutrient media, stimulating the synthesis of pioverdine, centrifugation and filtration of cultures for obtaining samples, sample irradiation with spontaneous ultraviolet radiation in the wavelength range of 200-300 nm, obtaining the fluorescence spectra, the resistance marker of the strain to antibiotics is the presence in a band of fluorescence of the peak in the wavelength range of 435-445 nm. The sensitivity marker of the strain to antibiotics is the presence in a band of fluorescence of the peak in the wavelength range of 455-470 nm.
EFFECT: invention enables to simplify the technology of optical methods of the study and to optimise treatment of bacterial infections.
SUBSTANCE: diagnosing a cause of an abnormal uterine haemorrhage is ensured by analysing a biological material that is menstrual supernatant dropped on a glass slide in an amount of 0.01-0.02 ml. It is dried at a room temperature for one day. The prepared sample is microscoped. If flagellated structures are found, the abnormal uterine haemorrhage is stated to have a tumour nature; while lingular structures enable diagnosing the abnormal uterine haemorrhage caused by an inflammatory process. The presented diagnostic technique enables identifying a cause of the haemorrhage and determining the optimum extent of treatment in a relatively short time using low amounts of biological fluids with minimum costs and high diagnostic sensitivity. The diagnostic procedure involves analysing that particular biological fluid which outflows from the involved organ directly, namely: menstrual discharge.
EFFECT: possibility to determine the nature of the pathological process directly in the endometrium and indentify the accurate cause of the abnormal uterine haemorrhage; the method is easy to implement.
2 dwg, 3 ex
SUBSTANCE: determined are: prothrombin time (PTT, sec) and serum level of selenium (SE mkg/l), as well as ultrasonic indices of the portal vein diameter (dVP, mm). A coefficient k is calculated in units by formula the probability of lethal outcome within 20 days is evaluated on the basis of the obtained result. If the values of the said coefficient k are higher than 3.8 units, lethal outcome is predicted, if k value is lower or equal 3.8 units favourable outcome is predicted, which makes it possible to continue treatment in the volume of the somatic department.
EFFECT: method makes it possible to increase the efficiency and reliability of diagnostics of cirrhosis decompensation and provide the timely correction of the carried out therapy.
1 tbl, 3 ex
SUBSTANCE: laboratory research is carried out. Sensitivity of channel apparatus to antidiuretic hormone, represented by desmopressinum in dose 0.15 mcg/100 g, is determined in animals under conditions of water dieresis with further carrying out morphological examination of kidney tissues.
EFFECT: application of claimed method makes it possible to diagnose lead nephropathology in experimental animal in absolutely accurate way.
2 dwg, 1 tbl, 1 ex
SUBSTANCE: invention relates to medicine, namely to clinical laboratory diagnostics, microbiological methods of research, and can be applied for standardisation of preanalytic stage of method of cuneate dehydration/crystallography for analysis of mixed saliva. Device includes table top, produced from extruded acryl glass, in bottom of which mounted are 4 metal legs in form of bolt with screw with moving on them nut, which make it possible to change angle of inclination of horizontal surface of table top by means of nut rotation, 2 levels for control of table top surface inclination angle l by axes X and Y and isolating lid with openings in side surfaces for uniform drying mixed saliva drop.
EFFECT: increase of standardisation quality is reached.
2 ex, 5 dwg
SUBSTANCE: invention relates to the field of medicine, namely to a method of predicting the development of severe sepsis. The essence of the method consists in the following: on the first day after acute poisoning with substances of narcotic action the content of procalcitonin, interleukin-6 and interleukin-8 is determined in blood serum. If the value of procalcitonin is from 0.5 ng/ml to 5 ng/ml, interleukin-6 from 20 pg/ml to 150 pg/ml, interleukin-8 from 50 pg/ml to 300 pg/ml, the development of severe sepsis is predicted.
EFFECT: application of the claimed method ensures the high reliability of prediction of severe sepsis development.
1 tbl, 3 ex
SUBSTANCE: group of inventions refers to medicine, namely to molecular diagnostics. A device for exposing a liquid sample to acoustic energy by generating cavitation in a liquid sample comprises a high-intensity ultrasonic wave source and a cartridge comprising a liquid sample and a liquid-air interface. The device is configured to focus high-intensity ultrasonic waves so that the cartridge integrated into the device generates a liquid well head above the liquid-air interface inside the cartridge, while a well head drop is a nucleation element. The group of inventions also refers to a system and a method for exposing the liquid sample to acoustic energy, as well as to a machine-readable medium with a software program for implementing the above method.
EFFECT: group of inventions provides probe processing by focused acoustic energy to generate the homogenous cavitation in the sample with reducing the desired energy and power.
9 cl, 9 dwg
SUBSTANCE: invention deals with a method of evaluating a threat of hypoxia formation in pregnant women with the aggravation of the cytomegaloviral infection in the third trimester of gestation. The essence of the method: in peripheral blood a titre of antibodies to cytomegalovirus is determined, the activity of phosphoenolpyruvate dehydrogenase is determined by the histochemical method, the content of discocytes, echinocytes, target-shaped erythrocytes, degenerative forms of erythrocytes, oxyhaemoglobine quantity are determined in peripheral blood. If the titre of antibodies to cytomegalovirus is 1:1600, detection of the reduction of phosphoenolpyruvate dehydrogenase activity to 12.0±0.09 c. u.: reduction of discocytes in peripheral blood to 74.0±1.3%, increase of the number of echinocytes to 7.0-0.2, target-shaped erythrocytes to 8.5±0.3%, degenerative forms of erythrocytes to 10.5±0.15% and reduction of the oxyhaemoglobine quantity to 92.0±1.6% a threat of formation of haemic hypoxia is evaluated.
EFFECT: improvement of the method of the threat evaluation.
FIELD: medicine, psychiatry.
SUBSTANCE: one should isolate DNA out of lymphocytes of peripheral venous blood, then due to the method of polymerase chain reaction of DNA synthesis one should amplify the fragments of hSERT locus of serotonin carrier gene and at detecting genotype 12/10 one should predict the risk for the development of hallucino-delirious forms of psychoses of cerebro-atherosclerotic genesis.
EFFECT: more objective prediction of disease development.
FIELD: medicine, urology.
SUBSTANCE: one should conduct subcutaneous prevocational tuberculin test and, additionally, both before the test and 48 h later it is necessary to perform the mapping of prostatic vessels and at decreased values of hemodynamics one should diagnose tuberculosis. The information obtained should be documented due to printing dopplerograms.
EFFECT: more reliable and objective information.
1 ex, 1 tbl
FIELD: molecular biology.
SUBSTANCE: the suggested innovation deals with the fact that nucleic acids should be isolated directly out of the sample without pipetting stage but with the help of interconnected reservoirs being prepared beforehand. The above-mentioned vessels should be applied either separately or being interconnected according to standard microtitrating format. The sample should be mixed with a lyzing buffer and nucleic acids are bound with matrix in closed system including, at least, two interconnected reservoirs. Forced movement of sample's mixture and buffer back and forth from one reservoir into another one for several times through narrow passage provides their thorough intermixing. The method provides quick and safe isolation of nucleic acids.
EFFECT: higher efficiency.
44 cl, 4 dwg, 1 ex
FIELD: medicine, phthisiology, microbiology.
SUBSTANCE: diagnostic material is poured preliminary with chlorohexidine bigluconium solution, homogenized, kept at room temperature for 10-12 h and centrifuged. Precipitate is poured with Shkolnikova's liquid medium, incubated at 37oC for 3 days, supernatant part of Shkolnokova's medium is removed, fresh Shkolnikova's medium is added, and precipitate is stirred and inoculated on the dense cellular egg media. Sensitivity of the strain is determined in 3 weeks by the presence of growth in the control tube only. Invention provides enhancing precision and reducing time for assay. Invention can be used in assay for medicinal sensitivity of tuberculosis mycobacterium.
EFFECT: improved assay method.
FIELD: medicine, biotechnology, pharmacy.
SUBSTANCE: invention relates to agents used for treatment of pathological states associated with disorder of synthesis of neuromediating substances. Method involves the development of pharmaceutical composition and a method for it preparing. Pharmaceutical composition represents subcellular synaptosomal fractions: synaptic membranes, "light" synaptosomes and "heavy" synaptosomes prepared from gray matter of cerebral hemispheres from experimental animals based on the goal-seeking modification of humoral mediators of nerve endings transformed to synaptosomes in development and regression of malignant processes. The composition provides inhibiting the growth of tumor cells, to elevate span-life of patients with ascite Ehrlich's sarcoma, breast adenocarcinoma Ca-755, Wolker's carcinosarcoma-256.
EFFECT: valuable medicinal and anti-tumor properties of composition.
12 cl, 3 tbl, 3 ex
SUBSTANCE: method involves carrying out microscopic examination of blood serum samples taken from femoral vein and cubital vein. Femoral vein sample is taken on injured side. The examination is carried out before and after treatment. The blood serum samples are placed on fat-free glass slide in the amount of 0.01-0.02 ml as drops, dried at 18-30°C for 18-24 h. The set of pathological symptoms becoming larger or not changed after the treatment in comparison to sample taken before treatment, and morphological picture of samples under comparison taken from the cubital vein showing no changes or being changed to worse, the treatment is considered to be effective.
EFFECT: enabled medicamentous treatment evaluation in course of treatment to allow the treatment mode to be changed in due time; avoided surgical intervention (amputation); retained active life-style of aged patients.
FIELD: medicine, clinical toxicology.
SUBSTANCE: at patient's hospitalization one should gather the data of clinical and laboratory values: on the type of chemical substance, patient's age, data of clinical survey and laboratory values: body temperature, the presence or absence of dysphonia, oliguria being below 30 ml/h, hemoglobinuria, erythrocytic hemolysis, exotoxic shock, glucose level in blood, fibrinogen and creatinine concentration in blood serum, general bilirubin, prothrombin index (PTI), Ph-plasma, the state of blood clotting system. The state of every sign should be evaluated in points to be then summed up and at exceeding the sum of points being above "+20" one should predict unfavorable result. At the sum of "-13" prediction should be stated upon as favorable and at "-13" up to "+20" - prediction is considered to be doubtful.
EFFECT: higher accuracy of prediction.
2 ex, 3 tbl
FIELD: medicine, juvenile clinical nephrology.
SUBSTANCE: disease duration in case of obstructive pyelonephritis should be detected by two ways: either by detecting the value of NADPH-diaphorase activity, as the marker of nitroxide synthase activity in different renal department and comparing it to established norm, or by detecting clinico-laboratory values, such as: hemoglobin, leukocytes, eosinophils, urea, beta-lipoproteides, lymphocytes, neutrophils, the level of glomerular filtration, that of canalicular reabsorption, urinary specific weight, daily excretion of oxalates, arterial pressure, and estimating their deviation against average statistical values by taking into account a child's age.
EFFECT: higher efficiency of detection.
7 dwg, 1 ex, 6 tbl
FIELD: medicine, urology.
SUBSTANCE: the present innovation deals with differential diagnostics of prostatic cancer and other prostatic diseases at the stage of primary inspection. The method includes the detection of PCA and calculation of probability coefficient for prostatic cancer (PCC) by the following formula: where e - the foundation of natural logarithm (e=2.718…), PCA - the level of total blood PCA in ng/ml, V - patient's age in years. At PCC value being above 0.2 one should diagnose prostatic cancer and to establish final diagnosis one should perform polyfocal prostatic biopsy. The method enables to increase accuracy of diagnostics at decreased number of unjustified prostatic biopsies.
EFFECT: higher efficiency of diagnostics.
FIELD: medicine, biology.
SUBSTANCE: invention relates to nutrient medium used for accumulation of cells for the following cytological and/or immunocytochemical analysis carrying out. Invention relates to medium containing salts NaCl, KCl, anhydrous CaCl2, MgSO4 x 6 H2O, MgCl2 x 6 H2O, Na2HPO4 x 2 H2O, KHPO4, NaHCO3, and also glucose and Henx's solution, 10% albumin solution and polyglucin taken in the ratio 1:1:1. Invention provides enhancing the preservation of cells.
EFFECT: improved an valuable properties of nutrient medium.