Method of determining fungicidal activity of chemical preparations

FIELD: agriculture.

SUBSTANCE: in agar plate which is in a Petri dish and inoculated with one of the fungal species of the genus Fusarium, circular holes are cut with the diameter of 8 mm, 0.05 ml of the working solution of the test preparation is applied into them, and placed in a thermostat at a temperature of 24.5-25.0°C, after 2-5 days depending on the type of fungus the diameter of the zone of growth inhibition of fungal mycelium is measured and the activity of the preparations is calculated according to the formula , and it is considered that when A=1 the fungicide is ineffective - the zone of growth inhibition is not formed (D=d), when A=2-3 the preparation activity is low, when A=4 it is average, and when A≥5 it is high.

EFFECT: invention provides accuracy of determining the activity of seed disinfectants and fungicides used in agricultural production.

1 tbl

 

The invention relates to agriculture and can be used to determine the fungicidal activity of chemical drugs against fungi causing plant diseases.

As a prototype the selected method used in medical research when studying the activity of the antibiotic agent is introduced into the wells on a nutrient medium, planted the studied species of bacteria or fungi. In the case of the presence of antibiotic action around the point of application of the drug is formed a rounded area of stunted growth of the microorganism. Largest diameter and evaluate the effectiveness of the test tools. This method allows to determine the activity of antibiotic substances against many types of microorganisms that cause disease in humans and animals (Birger M. O. and others Handbook of microbiological and virological research methods. - M: Medicine. - 1982; beat V. I., Methods of experimental Mycology: a Handbook. - Kiev. "Naukova Dumka". - 1982; Egorov N. With. Basic knowledge about antibiotics: a Training manual. - M.: Higher school. - 1979).

The main disadvantage of this method is that in medicine to evaluate the activity of the drug is used to the average diameter of the zone of restricted growth". The proposed method is based on determining the activity of a fungicide on a specially designed �matematicheskoi the formula and the assessment of this indicator is designed for scale, allowing you to more objectively evaluate the effectiveness of disinfectant.

Technical problem - ensuring the accuracy of the new method using mathematical formulas and scoring scales for determining the activity of seed disinfectants and fungicides used in agricultural production.

To achieve the technical objectives of the special experiments, the mathematical formula and is composed of the evaluation scale to determine the activity of fungicides.

The experiments were conducted as follows: in the agar plate in a Petri dish and sown with one species of fungi of the genus Fusarium, cut round holes with a diameter of 8 mm. they were added 0.05 ml of the working solution of the test drug. The working solution of fungicide were prepared the same concentration as in the production environment. Petri dishes with a culture of the fungus caused by the fungicide was placed in a thermostat at a temperature of 24.5-25,0°C. in 2-5 days depending on the type of fungus, carried out measurements of the diameter of the zone of stunted growth of fungal mycelium and the expected activity of the preparations according to the formula:

A=Dd,(1)

where A is the activity of the fungicide (in arbitrary units);

D - diameter of the zone of stunted growth of fungal mycelium (mm);

d - diameter of the application site fungicide solution (mm) equal to 8 mm.

Grading scale consists of the following values: if A=1 fungicide ineffective area of stunting is not formed (D=d); if A=2-3 activity of the drug is low; when A=average 4 and A≥5 high. Formula (1) shows how many times the diameter of the zone of stunting greater than the seat diameter of the preparation (the diameter of the hole).

The results of application of the method for determining the activity of fungicides is presented in the table.

td align="left"> 5,6
Activity of fungicides - seed disinfectants against fungi of the genus Fusarium
№ p/pDrug consumption normThe activity of the drug (A)
the fungal species
F. avenaceumF. culmorumF. graminearumF. poae
1Vincit SC, 2 l/t3,94,97,65,0
2Maxim COP, 2 l/t6,06,44,0
3Maxim old COP, 1.5 l/t5,85,18,23,9
4Kolfage super COP, 2 l/t2,44,26,93,5
5Vitaros FAC, 3 l/t2,63,92,02,5
6Raxil COP, 0.5 l/t2,01,42,92,5
7Dividend the old CS,1 l/t1,92,21,02,2
8Premis COP, 2 l/t1,02,81,01,0
9Control (sterile water) 1,01,01,01,0

As the study results show that the tested drugs can be divided into several groups. The first high and close to the average activity (3,9-8,2) includes Vincit IC, Maxim COP and the old Maxim of the COP. Second is Kolfage super COP. Its activity against species of Fusarium graminearum and F. culmorum was high and average (6,9 and 4.2), F. avenaceum and F. roue - low (2.4 and 3.5). The third group includes the fungicides Vitaros VSC and Raxil the COP. An indicator of the activity of the first drug against F. culmorum was close to the average of (3.9) with respect to other types vusalevich mushrooms these funds were at a low level (from 1.4 to 2.6). The latter group includes the Dividend of the old COP and Premis the COP. The activity of these drugs was low (A=1,9-2,8) or absent (A=1).

Thus, the use of the method for determining the activity of fungicides against fungi of the genus Fusarium with application of the formula and the new scale provides a more accurate assessment of the test drugs and recommend them for further study or use in agricultural production.

The effectiveness of this method is expressed in a more precise determination of the fungicidal activity of chemical drugs compared to the diameter of the zone delay� growth."

Method of determining the fungicidal activity of chemical preparations, characterized by the fact that in agar plate in a Petri dish and sown with one species of fungi of the genus Fusarium, cut round holes with a diameter of 8 mm, making of 0.05 ml of the working solution of the test drug and placed in a thermostat at a temperature of 24.5-25.0°C, 2-5 days depending on the type of fungus measurements of the diameter of the zone of stunted growth of fungal mycelium and calculate the activity of the preparations according to the formula:

where: a - active fungicide (in arbitrary units);
D - diameter of the zone of stunted growth of fungal mycelium (mm);
d - diameter of the application site fungicide solution (mm) equal to 8 mm,
and consider that if A=1 fungicide ineffective area of stunting is not formed (D=d), if A=2-3 activity of the drug is low, A=average 4 and A≥5 high.



 

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