Method of controlling undesirable herbaceous vegetation of class dicotyledones

FIELD: agriculture.

SUBSTANCE: invention relates to agricultural microbiology, in particular to protection of plants against invasive and perennial weed vegetation of class Dicotyledones. The suspension is used on the basis of vegetative mycelium of phytopathogenic micromycetes with chemical herbicides in sublethal doses. The plants are treated by spraying an aqueous solution of the herbicide with the active substance of glyphosate, and suspension of mycelium of foma-like fungi. Treatment is carried out at all stages of plant development, first with herbicide at the flow rate by glyphosate active substance of 0.02-0.32 kg/ha, then by a suspension based on mycelium fragments of foma-like fungi in a concentration of from 25 to 100 g/l. The consumption rate of the working fluid is from 50 to 300 l/ha. The proposed method enables to reduce 1.5-10 times or more the chemical load on the ecosystem, 1.5-4 times the infectious load (concentration of phytopathogenic fungi mycelium).

EFFECT: method can be applied at all stages of plant development, without requiring additional protection of the cultivated crop.

10 tbl, 5 ex

 

The invention relates to agricultural Microbiology, in particular to the protection of plants from invasive and weeds by successive application at the beginning of the chemical herbicide in sublethal doses, and then the suspension on the basis of vegetative mycelium of phytopathogenic Thomas-like micromycetes.

A known way of dealing with bipartite (class Dicotylédones) invasive and long-soboliferous weeds is a method of processing chemical herbicides (Public folder..., 2012 [7]). However, the negative consequences of the use of chemical herbicides, as well as the emergence of resistant populations of such plants affects the environment and leads to a decrease in the efficiency of this method.

As an alternative to chemical herbicides to combat hard-extirpated, the dominant species of weeds the most promising are the preparations on the basis of phytopathogenic fungi (TeBeest, 1996; Gasich, berestecky, 2007 [24, 4]). Their advantages include environmental security, high specificity, the duration of the aftereffect, as well as the ability to share with some chemicals and phytophagous (Leathers et all., 1993; Gressel, 1997 [21, 19]). However, the range of developing biological control agents such dicotyledonous (class Dicotylédones) Rast is s limited (berestecky, Sochorova, 2009; De Faria,Wright, 2007 [3, 17]), moreover the efficiency of their application is highly dependent on temperature and humidity conditions (Leathers et all., 1993; Sochorova, 2011; Sochorova, Berestecky, 2012 [21, 10, 11]).

Thomas-like fungi - a group of phytopathogenic fungi, United by common morphological features and ways of infection targets that currently includes the genus Phoma, Phomopsis, Stagonospora, etc. (Boerema et al., 2004 [14]). The suspension on the basis of fragments of mycelium Thomas-like micromycetes is environmentally safe in most cases, aggression is primarily to species in the same family of dicotyledonous plants (Kungurtseva et al., 2006; Gasich and others, 2010; Sochorova, 2011 [20, 5, 10]).

The known method of using fragments of myceliumPhoma macrostomato combat dicotyledonous plants of the Thistle field(Cirsium arvenseL.) and dandelion{Taraxacum officinaleF. H. Wigg) (Bailey&Derby, 2006, [12]). Mycelium was obtained in the grain substrate and 100% plant death was observed by adding crushed, overgrown with mycelium substrate into the soil with a consumption rate of 1 kg/m2and high consumption of mycelium - 100 mg/ml, which indicates the complexity of applications and the large consumption of the drug (Bailey&Derby, 2006, [12]).

Conducted ecofloristic studies have revealed a highly aggressive in terms of the Thistle field{Cirsium arvense L.) strain 163 micromyceteStagonospora cirsiiJ. J. Davis (berestecky, 2005 [1]) and strain 17.7 micromycetePhoma exigua var. exiguaSacc.{=Ascochyta sonchi(Sacc.) Grove) (Levitin et al, 2007 [22]), and in the case of Sosnowski's Hogweed{Heracleum sosnowskyiManden.) the strain of 1.40 micromycetePhoma complanata(Tode:Fr.) Desm.. (Gasich and others, 2010 [5]). It was found that strains of these Thomas-like micromycetes can virulent form mycelium able to successfully infect the target plants with a 24-hour period of high humidity and lower than the infecting spores, infection load (Gasich and others, 2010; berestecky, 2005 [5, 1]). It was also shown that these micromycetes have narrow specialization (Kungurtseva et al., 2006; Gasich and others, 2010; Sochorova, 2011 [20, 5, 10]). However, the use of suspensions on the basis of these micromycetes in the field, the observed efficacy of the use was in a strong dependence on temperature and relative humidity, and demanded high concentrations of fragments of mycelium suspension (berestecky, 2005; Gasich and others, 2010; Sochorova, 2011 [1,5,10]).

Glyphosate is non-selective systemic herbicide used primarily to combat perennial weeds class Dicotylédones. Apply by spraying of vegetating weeds in spring or summer (subject to the protection of culture) at a flow rate of working fluid - 25-200 l/ha WMO is it as active substances in drugs Tornado, Tornado 500, Alas, Default, Dominator, Foset, Fighter, Pan, cosmic, Gligor, Glyfos, Glyfos Premium, octopus, Torch, Argument, ground, Single, zero, Total, Round, Typhoon, glider, Glider, Glyph Alt, Zeus, Cayman, Hurricane Extras, octopus Extras and so on, the Concentration of the active substances in drugs used in the field, from 360 to 540 g/l (the State..., 2012 [7]). Thus, when applying glyphosate against dicotyledonous perennial soboliferous weeds and invasive plants recorded from 13.5 to 108 kg/ha

A known way of dealing with the Thistle field (Cirsium arvenseL.) adopted for the prototype, which used a suspension on the basis of mycelium phytopathogenic fungusStagonospora cirsiiDavis received on the grain substrate or Saharsa-soy medium, at a concentration of 75 mg/ml in conjunction with chemical herbicides based on glyphosate at concentrations of 0.1-0.4 mg/ml (Sochorova, 2011 [10]). The consumption rate in the prototype from 200 to 1000 l/ha the biological efficiency was close to 100%. However, the method requires a greater consumption of chemical herbicide and infectious load of the fungus, resulting in more chemical load on the ecosystem, leads to increased production of mycelium and complicates the application of the suspension by spraying. Furthermore, the prototype has not been studied selectivity of such use. At the same time, Russ is atrendy way maybe as in the prototype, applied at all stages of plant development.

The objective of the invention is to increase the efficiency of the method for invasive and long-soboliferous plants class Dicotylédones, by applying environmentally friendly means on the basis of vegetative mycelium Thomas-like fungi in minimal doses of herbicide and concentration of mycelium.

The problem is solved by a method consisting in spraying the plants with an aqueous solution of the herbicide with the active ingredient glyphosate and suspension of mycelium Thomas-like fungi with surfactant, wherein the treatment is carried out at all stages of plant development, sequentially, first with the herbicide at the rate applicable to the substance glyphosate 0.02 to 0.32 kg/ha, and then the suspension on the basis of fragments of mycelium Thomas-like fungi, containing a surface-active substances (surfactants) - tween-80 or Silwet L-77, and the following ratio, wt.%:

Fragments of mycelium Thomas-like fungi2,5-10
(if the initial concentration is not less than 0.5×106CFU/g)
Tween-80 or Silwet L77of 0.05 - 0.2
the ode the rest,

thus the rate of flow of the working fluid is 50-300 l/ha

The consecutive application at the beginning of chemical herbicides based on glyphosate, and then the suspension on the basis of the crushed mycelium Thomas-like fungi increases the effectiveness of actions in respect of all dicotyledonous plants within families, dedicated to the pathogenic fungi, the mycelium which is part of the suspension.

Practical applications illustrate the following examples, which demonstrate the high efficiency of this method of struggle against dicotyledonous undesirable vegetation that reduce the chemical load on the ecosystem and demonstrating the selective action within the collection.

Example 1.The influence of the sequence of application of chemical herbicide and suspension on the basis of mycelium Thomas-like fungus on the degree of destruction of ground part of dicotyledonous plants: creeping Thistle, field and Sosnowski's Hogweed

Virulent mycelium Thomas-like fungi strains 163S. cirsii,or strain 17.7Ph. exigua var. exigua,go strain 32.121Ph.complanatareceived on Saharsa-soy medium (Sokornova, Berestetskiy, 2007; Gasich and others, 2010; Sochorova, 2011 [23, 5, 10]). The resulting mycelium together with the remnants of the nutrient medium crushed blunderingly MR 6550 BC-HC-M 40 seconds at 10000 rpm Received a "breeding" suspension on the basis of fragments of mycelium Thomas-like fungi contained viable units of not less than 0.5×106CFU/g (table 1). Then this suspension was diluted with a solution of 0.01% tween 80 to a final concentration of fragments of mycelium of 0.5×104CFU/g (10% solution).

The number of viable units were determined by conventional mycological methods (Methods, 1982 [8]).

The pathogenicity of mycelium was estimated by the area of necrosis on the leaf disks (Berestetskiy et al., 2007 [13]).

The solution of the chemical herbicide Tornado BP (active ingredient: glyphosate) was prepared by diluting the concentrate (initial concentration of 360 g/l) to a concentration of 0.2 g/l Working solution was applied by spraying plants in the rosette with a consumption rate of 60 ml/m2as for herbicide and suspension of the fungus. Consistently applying the processing conducted by spraying in a row, without any time intervals. The efficiency of destruction of plants was estimated by the area of necrosis of leaves on day 7 after treatment in field conditions at the temperature of 20-25°C and 65-85% relative humidity.

As can be seen from table 2, most reliably defeat a bipartite target plants (Thistle field and Sosnowski's Hogweed) was observed consistently applying at the beginning of the chemical herbicide, the m suspension on the basis of mycelium Thomas-like micromycetes. In this area the defeat leaves of the Thistle field consistently applying the suspension on the basis of fragments of myceliumS. cirsiiand chemical herbicides based on glyphosate is 95%, which is commensurate with the prototype, but reducing the concentration of mycelium suspension by 25%, and glyphosate in the working solution of the chemical herbicide, more than 10 times (Sochorova, 2011 [10]). As can be seen from table 2, consistent application significantly increased the efficiency of the method of application that has opened the possibility to use other types Thomas-like fungi in this way decreasing the consumption norms.

Example2.The nature of the interaction of glyphosate and mycelium Thomas-like fungi consistently applying in respect of dicotyledonous plants: creeping Thistle, field and Sosnowski's Hogweed

The basis for working solutions were prepared as in example 1. Then it was diluted up are shown in table 3, 4, 5 concentrations. The rate of flow of working solutions and the environmental conditions were as in example 1. Processing plants in the rosette was carried out by spraying sequentially without any time intervals: glyphosate at a concentration of from 0 to 0.54 g/l, then the suspension on the basis of mycelium Thomas-like micromycetes concentrations of 0, 12, 5, 25 and 50 g/l

As can be seen from table 3, a synergistic effect when consistently is applied at the beginning of the chemical herbicide Tornado BP (active ingredient: glyphosate), and then the suspension on the basis of mycelium of strain 163S. cirsii,occurs when the concentration of glyphosate in the current matter, 0.05 and 0.1 g/l at a concentration of mycelium 25 and 50 mg/ml, allowing you to get the efficiency in respect of the Thistle field close to the observed in the prototype (90 and 94%), lowering the concentration of glyphosate in 6 times and concentration of mycelium 1.5 and 2 times as compared with the prototype. As can be seen from table 4, close to 100% efficacy against invasive plants of Sosnowski's Hogweed see consistently applying at the beginning of the chemical herbicide Tornado BP (active ingredient: glyphosate), and then the suspension on the basis of mycelium of strain 32.121Ph.complanata.The concentration of the chemical herbicide below 10 times, and the concentration of mycelium 1.5 and 2 times as compared with the prototype. As can be seen from table 5, close to 100% effectiveness against Thistle field see consistently applying at the beginning of the chemical herbicide Tornado BP (active ingredient: glyphosate), and then the suspension on the basis of mycelium of strain 17.7Ph. exigua var. exigua.The concentration of the chemical herbicide below 1.5 times, and the concentration of mycelium in 2 and 4 times compared to the prototype. In the case of chemical herbicide concentration more than 100 times below the recommended for dealing with these types of Megilat is through vegetation (State..., 2012 [7]).

Analysis of the effectiveness of the consistent application of chemical and biological agents is estimated to achieve a synergistic effect. A synergistic effect occurs when the effect of the active ingredients of the combination of compounds exceeds the total (additive) action of active ingredients applied individually. Classical calculation of the expected impact of the combination of the two herbicides (We) proposed Colby and conducted according to the following formula:

We=(100-XAnd)·(100-Min)/100,

where XAnd-effects when treatment with herbicide And at the rate equal to RAndexpressed in percent, XB-effects when treatment with the herbicide At the rate equal to RBexpressed in percent (Colby, 1967 [16]).

Estimated exposure is compared with the value observed in the joint application of herbicides a and b at the same rate of component pAndand RB, respectively. To do this, calculate the level of interaction R as the ratio of the expected and observed impacts. According to Colby additive interaction occurs when R=1, synergism is observed when R>1, and antagonism when R<1. However, biological systems are characterized by a wide range of options, therefore it is considered that significant synergies in R>1.5, and the antagonism of p and R< 0,5. Accordingly, when the values of R from 0.5 to 1.5 interaction is treated as additive (Gisi et all., [18]).

All the suspension on the basis of mycelium Thomas-like fungi compatible with chemical herbicide with glyphosate in sublethal doses. Moreover, if properly applied at the beginning of glyphosate, then composition on the basis of mycelium Thomas-like fungi in relation to the target dicotyledonous plants are observed to have a synergistic effect, leading to comparable with the prototype efficiency while reducing consumption rates of chemical and mycelium suspension.

Example 3.Consistent application of chemical herbicides based on glyphosate and suspension on the basis of fragments of mycelium Thomas-like micromycetes with the boundary and beyond the values of the component

The suspension on the basis of mycelium Thomas-like fungi: strain 163S. cirsii,or strain 17.7Ph. exigua var. exigua,or strain 32.121Phcomplanataand the working solution of chemical herbicides based on glyphosate were prepared as in examples 1, 2. Treated at the temperature of 20-25°C and 65-85% relative humidity of dicotyledonous plants in the rosette by spraying at a rate of 60 ml/m2at the beginning of the herbicide Lontrel (active ingredient glyphosate 360 g/l), then the suspension on the basis of fragments of mycelium of the fungus. As can be seen from the tables is 6, using the minimum amount of herbicide on the basis of glyphosate (0.02 kg/ha under current substance), 20 times smaller than the prototype, and the concentration of suspension on the basis of the mycelium is 25% higher than in the prototype, the area of necrosis in 15% higher than when using the suspension on the basis of mycelium Thomas-like fungi without chemical herbicide in the prototype, and 25% lower than when using 20 times as much chemical load. The maximum according to this invention the concentration of a chemical herbicide and concentration of mycelium suspension comparable to the prototype and cause 100% damage plants.

Reducing the lower limit concentrations (very high concentration) of the chemical herbicide and / or mycelium suspension leads to a low efficiency (area of necrosis of leaves less than 20%) and initiates a target resistance of plants to chemical herbicides based on glyphosate. The applied rates of chemical herbicide with the active ingredient glyphosate above the maximum limit, not increasing efficiency, leads to the expansion of a circle struck cultivated plants, to increase the chemical load on the ecosystem. Increasing the concentration of mycelium suspension above 100 mg/ml does not increase the efficiency, and therefore leads to unreasonable consumption of mycelium. In addition, due to the increase of the density of the suspension can occur is whether clogging of the nozzle of the spray equipment.

Example4.The selectivity of the method consistent application of chemical herbicide and suspension on the basis of mycelium Thomas-like fungi

The suspension on the basis of mycelium Thomas-like fungi and working solution on the basis of chemical herbicides based on glyphosate were prepared as in examples 1, 2. Evaluation of the selectivity of the method of application of chemical herbicide and suspension on the basis of the mycelium of the strain 163S. cirsii,or strain 17.7Ph. exigua var. exigua,or strain 32.121Phcomplanataconducted on the area of necrosis in a sequential processing by spraying plants in the rosette at the beginning of the suspension. Score "+++" corresponds to an area of necrosis of leaves from 75 to 100%, "++- " corresponds to an area of necrosis of leaves from 50 to 75%,"+--"corresponds to an area of necrosis of leaves from 25 to 50% and "---" less than 25%. As can be seen from tables 7, 8, 9, consistent application of chemical herbicides based on glyphosate and suspension on the basis of myceliumPhcomplanata, S. cirsiiandPh. exigua var. exigua,does not increase the number of plant families class Dicotylédones, smitten by the fungus, but extends the range of host plants within the family. As can be seen from tables 7, 8 and 9 consistent application of chemical herbicide (target substance glyphosate) and the suspension on the basis of mycelium of strain 163S. cirsii,or strain 17.7Ph. exigua var. exiguaallow you to plug the et also with efficiency, comparable to the prototype, to deal with such weeds like Thistle gray and pink sow Thistle, and suspension on the basis of strain 32.121Phcomplanatatwo species of giant Bolshevikov(Heracleum mantegazzianumSomm. et Levier,H. sosnowskyiManden). Thus, the effectiveness of compositions on the basis of mycelium Thomas-like fungi on dicotyledonous plants increases, and the selectivity does not change that allows discussed ways to deal with a lot of undesirable herbaceous vegetation. In the prototype and its analogues, this option was not considered.

Example5.The flow rate of the working solution of suspension on the basis of mycelium Thomas-like fungi of the consecutive application of chemical herbicide and mycelial suspensions

The suspension is based on the basis of the mycelium of the strain 163S. cirsii,or strain 17.7Ph. exigua var. exigua,or strain 32.121Phcomplanataand working solution-based chemical herbicide Tornado (active ingredient glyphosate at a concentration of 360 g/l) was prepared as in example 1. In the example used mycelial suspension at a concentration of 25 g/l, containing 0.2% solution of the surfactant Silwet L-77. The concentration of the target substance glyphosate in solution-based chemical herbicide Tornado was 0.2 g/L. For this Tornado was diluted with distilled water by after batelling cultivation in 1800 times.

As to the different phases of development of the target plants have different size ground part, moreover described in this invention giant Bolsheviki differ in that their ground part reaches 3 meters (Handbook..., 2013, [9]), the required volume of working solutions were calculated so that the suspension was drip distributed over the entire surface of the leaves. In the experience of cultivated plants of the Thistle field in the phase sockets (average plant height 10 cm) at a density of 9 plants/m2, Thistle field in the flowering phase (average plant height 25 cm) at a density of 4 plants/m2and Sosnowski's Hogweed in the flowering phase (average plant height of 1 m 20 cm) at a density of 4 plants/m2. As can be seen from table 10, when the manual spraying with a hand sprayer STIHL SG 10 (Germany) consumption working solutions, leading to the death of plants was 50 l/ha for Thistle field and 300 l/ha for Sosnowski's Hogweed.

Way of dealing with difficult icerenkoy unwanted dicotyledonous vegetation, such as long-soboliferous weed plants(Cirsium arvense(L.) Scop. s.I. (=C.setosum(Willd.) Besser ex M. Bíeb.),C. incanum(S. G. Gmel.) Fisch.),Sonchus arvensisL.) and invasive plants, in particular the giant Bolsheviki(Heracleum mantegazzianumSornm. et Levier,H. sosnowskyiManden andH. persicum Desf.), reduces chemical load on the ecosystem of 1.5 - 10 times, infectious load (concentration of phytopathogenic fungi mycelium) 1.5 - 4 times compared to the prototype. The method can be applied at all stages of plant development, not requiring additional protection of cultivated crops.

Table 1.The output of the mycelium Thomas-like fungi and pathogenic properties under cultivation during the3days on Saharsa-soy environment

Table 3.The nature of the interaction of glyphosate and myceliumS. cirsiiconsistently applying in respect of dicotyledonous plants of the Thistle field(Cirsium arvenseL.)

Table 4.The nature of the interaction of glyphosate and myceliumR. complánalaconsistently applying in respect of dicotyledonous plants Sosnowski's Hogweed(Heracleum sosnowskyiManden.)

Table 6.Consistent application of chemical herbicides based on glyphosate and suspension on the basis of fragments of mycelium Thomas-like micromycetes the limit values of glyphosate and mycelial suspensions

,

Table 10.The flow rate of the working solution of suspension on the basis of mycelium Thomas-like fungi of the consecutive application of chemical herbicide and mycelial suspensions

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The method of combating unwanted vegetation class Dicotyledones by processing plants by spraying an aqueous solution of the herbicide with the active ingredient glyphosate and suspension of mycelium Thomas-like fungi with surfactant, wherein the treatment is carried out at all stages of plant development sequentially, first with the herbicide at the rate applicable to the substance glyphosate 0.02 to 0.32 kg/ha, and then the suspension on the basis of fragments of mycelium Thomas-like fungi, containing a surface-active substances (surfactants) - tween-80 or Silwet L-77, and the following ratio, wt.%:
Fragments of mycelium Thomas-like fungi

(if the initial concentration is not less than 0.5×106CFU/g)2,5-10
Tween-80 or Silwet L-77of 0.05-0.2
Waterthe rest,

thus the rate of flow of the working fluid is 50-300 l/ha



 

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FIELD: chemistry.

SUBSTANCE: group of inventions relates to biotechnology. A method of obtaining a granulated product includes growing filamentous fungi of a Monilialeae family, preferably Arthrobotrys conoides Dreschsler in a suitable liquid culture medium. The obtained culture of the filamentous fungus is mixed with at least one type of modified starch and starch flour, with the modified starch and modified flour being present in weight ratios, constituting from 30:70 to 60:40. Filling agents and in case of necessity nutritional substances are added to the obtained mixture with obtaining a paste. The granulation of the paste is carried out. The obtained granules are dried until the moisture level lower than 13% is achieved, preferably to the level constituting from 9 to 10%. The granulated product, obtained by the method described above, is used for the application in a pesticidal composition.

EFFECT: group of inventions provides obtaining the target product with high dispersability and a high percent of survival of the filamentous fungi propagules.

9 cl, 1 dwg, 4 ex

FIELD: chemistry.

SUBSTANCE: group of inventions relates to biotechnology. The method of obtaining a biological preparation for the protection of plants against phytopathogens and nematodes based on a genus Trichoderma fungus strain is realised by preparation of an inoculation material of the fungus strain, making the preparation in a liquid or friable form based on the inoculation material, and mixing the preparation with a mineral, organic or bacterial fertiliser.

EFFECT: biological preparation, resulting from the method, possesses the high antagonistic activity with respect to a wide spectrum of phytopathogens and nematodes.

6 cl, 5 ex

FIELD: chemistry.

SUBSTANCE: group of inventions relates to biochemistry. Claimed is a device for surface growing of a microorganism on a liquid culture medium. The device includes a dish with a drain pan for growing of the microorganism, provided with a transport net freely dropped on its bottom. The transport net is made from a neutral material and is fastened by its ends to drive drums, which ensure lifting of the transport net from the dish bottom. The device also contains a reception device with a valve for the connection to a mixer-doser and supply of the liquid culture medium mixture with mother culture into the dish, rotary flaps, placed on butt ends of the dish for the regulation of air exchange in the dish, a drain device for pouring out the culture liquid, a pipe branch for the supply of a drying agent under the net, a sampling instrument and removable colour filters on lamps for the regulation of illumination, placed on side walls of the dish, a lid, which hermetically closes the dish, made with a possibility of its opening and/or removal, equipped with injectors for spraying the SAS solution above the surface of biomass growing in the dish and washing heads for the realisation of sanitary processing of the device. Also claimed is a method of surface growing of a microorganism on a liquid culture medium with the application of the claimed device.

EFFECT: claimed inventions ensure growing of microorganisms on the liquid culture medium with the minimal application of manual labour.

7 cl, 1 dwg, 2 ex

FIELD: biotechnology.

SUBSTANCE: wound-healing agent is a concentrate of the culture liquid of strain Trichoderma harzianum Rifai deposited in the Russian National Collection of Industrial Microorganisms under the number of RNCIM: F-180, as a producer of L-lysine of alpha-oxidase, and can be applied as a wound-healing agent for skin lesions.

EFFECT: invention enables to expand the range of means that provide wound healing of skin lesions.

1 tbl, 2 ex

FIELD: biotechnology.

SUBSTANCE: invention relates to the use of the concentrate of the culture liquid of the strain Trichoderma harzianum Rifai, deposited in the Russian National Collection of Industrial Microorganisms under the number of RNCIM F-180 as an inhibitor of Andis virus of potato mottling.

EFFECT: invention enables to reduce losses of potato from the plant infection with the Andis mottling virus.

2 ex

FIELD: biotechnology.

SUBSTANCE: nutrient medium for growing filamentous fungi-dermatophytes from clinical material comprises glucose, bacteriological agar, meat peptone, casein hydrolyzate, yeast extract, sodium chloride, sodium carbonate, L-cysteine, thioglycolic acid and distilled water in a predetermined ratio of components.

EFFECT: invention enables to reduce time of growing filamentous fungi-dermatophytes from clinical material.

1 tbl, 2 dwg, 4 ex

FIELD: biotechnologies.

SUBSTANCE: method provides for pre-seeding treatment of seeds, soil irrigation and treatment of vegetative parts of plants with cultural liquor of strain Lactobacillus plantarum 60-DEP deposited in the All-Russian State Collection of Strains of Microorganisms, which are used in veterinary science and animal breeding, with a titre of 106 CFU/ml at the consumption of 5-30 ml per 100 ml of water.

EFFECT: invention allows simplifying the method.

8 tbl, 3 ex

FIELD: biotechnology.

SUBSTANCE: use of the enzyme of ribonuclease Bp as an inhibitor of development of RNA-genomic phytoviruses is proposed. In processing tobacco plants with ribonuclease Bp enzyme solution with the concentration of 100 µg/ml of distilled water, 94% of the plants are resistant to X-virus of tobacco.

EFFECT: invention is highly effective in inhibiting RNA-genome phytoviruses.

11 cl, 8 tbl, 6 ex

FIELD: agriculture.

SUBSTANCE: method of production of plant growth stimulant is carried out by hydrolysis of cattle manure under aerobic and anaerobic conditions, using the seed material at different stages of the technological process of specially selected associations of lactobacilli and indigenous methanogenic microorganisms. All stages of the technological process are carried out continuously using a detachable-filling technology of the process that provides complete replacement of raw material in the bioreactor for 7-10 days.

EFFECT: obtaining liquid organic fertilisers, disinfected from pathogenic microflora, free from weed seeds and with low odour threshold, complete preservation of nutrients which are in the initial manure drain by storing all nitrogen in ammonium or organic forms, phosphorus is mainly in the form of phosphatites and nucleoproteins, and potassium is in the form of soluble salts, which provides their better accessibility by plants.

3 cl, 2 tbl

FIELD: chemistry.

SUBSTANCE: group of inventions relates to biotechnology. Disclosed is a Bacillus subtilis VKPM V-11353 strain, capable of splitting a wide range of mono- and di-sugars and a wide range antagonistic effect on pathogenic and opportunistic pathogenic bacteria and fungi, which cause diseases in plants and farm animals. Also disclosed are versions of using the Bacillus subtilis VKPM V-11353 strain as a bacterial preservative for silos, for producing agents for normalising intestinal microflora of animal farms and for producing agents for protecting plants from diseases.

EFFECT: group of inventions improves the quality of silos, improves safety of animal farms and increases effectiveness against pathogenic and opportunistic pathogenic microorganisms which cause wheat diseases.

4 cl, 8 tbl, 4 ex

FIELD: biotechnology.

SUBSTANCE: strain of bacteria Paenibacillus sp. IB-1 has antagonistic activity against phytopathogenic fungi. The strain is deposited in the Russian National Collection of Microorganisms under the registration number VKM B-2823D and can be used to produce the biological product for protection of plants against diseases caused by phytopathogenic fungi.

EFFECT: invention enables to reduce the duration of cultivation and increase the yield of microbial biomass.

4 tbl, 4 ex

FIELD: medicine, pharmaceutics.

SUBSTANCE: invention refers to biotechnology, namely to protective devices for humans and farm animals against blood-sucking mosquitoes. The strain Bacillus thuringiensis var. israelensis No. 7-1/23A possesses larvicidal properties. The strain Bacillus thuringiensis var. israelensis is deposited in the collection of State Scientific Institution All-Russian Research Institute of Agricultural Microbiology of Russian Agricultural Academy under No. RCAM00626 in the group of spore microorganisms. It can be used in creating a larvicidal biopreparation against blood-sucking mosquitoes.

EFFECT: invention enables providing higher death rate of blood-sucking mosquitoes.

5 tbl, 1 ex

FIELD: agriculture.

SUBSTANCE: agent for microbiological protection of plants comprises a mixture of culture liquids Trichoderma viride, Azotobacter chroococcum, Bacillus megaterium, Beauveria bassiana, Metarhizium anisopliae with the necessary amount of water. The ratio of cultures in the mixture is 1:1:1:1:1, with a titre of each culture is not less than 1×107 CFU/ml. Using the said agent the method of microbiological protection of plants is implemented, comprising 2-3-fold spraying of plants during the growing season at a dosage of 12.5 l of the agent per 1 ha of crops. Spraying is carried out when the average temperature of air is not less than 15°C with an interval of 2-3 weeks.

EFFECT: stimulation of plant growth, increase in plant yield, improvement of soil fertility.

2 cl, 5 tbl, 4 ex

FIELD: biotechnologies.

SUBSTANCE: Bacillus subtilis strain - I5-12/23 has the antagonist activity with reference to phytopathogenic funguses and bacteria. The strain is reposited in GNU VIZR State Collection of Microorganisms under registration number Bacillus subtilis 93 VIZR also can be used for protection of potatoes against illnesses during storage.

EFFECT: improved preservation of potato tubers.

5 tbl

FIELD: agriculture.

SUBSTANCE: invention relates to the field of agriculture and biotechnology, in particular to plant-growing, and can be used at cultivation of plants of leguminous cultures. The method consists in that seeds before sowing are wetted in 2 % water solution of ferment preparation Tsello-Lux F within 8-10 hours.

EFFECT: invention allows to improve germinating capacity of seeds and to increase intensity of growth at the beginning of vegetation of leguminous cultures.

2 tbl

FIELD: chemistry.

SUBSTANCE: as an anticoagulant the composition contains difetialon or brodifacoum or bromadiolone or diphenacum or ethylphenacine, as a gel-forming substance it contains mixture of xanthan or locust bean gum, taken in a weight ratio equal to (1-9):(1-9), respectively, or a mixture of carrageenan and locust bean gum, taken in a weight ratio equal to (1-9):(1-9), respectively, or a mixture of xanthan and guar gum, taken in a weight ratio equal to (1-9):(1-9), respectively, as a stabiliser it contains polyethyleneglycol, as a dye - carmine, it also contains water, or milk, or lactoserum, with the following component ratio, wt %: anticoagulant - 0.005-0.01, mixture of xanthan and locust bean gum, taken in a weight ratio equal to (1-9):(1-9), respectively, or a mixture of carrageenan and locust bean gum, taken in a weight ratio equal to (1-9):(1-9), respectively, or a mixture of xanthan and guar gum, taken in weight ratio equal to (1-9):(1-9), respectively, - 0.3-15, stabiliser - 3.0-20, dye - 0.02-0.05, water, or milk, or lactoserum - the remaining part.

EFFECT: invention makes it possible to efficiently apply the preparation at minus temperatures.

12 cl, 318 ex

Antibiotic peptides // 2472805

FIELD: chemistry.

SUBSTANCE: peptides and peptide derivatives have general formula Sub1-X1NX2X3PVYIPX4X5RPPHP-Sub2, where Sub1, X1, X2, X3, X5, Sub2 are given in the claim. The disclosed peptides or peptide derivatives have at least one of the following advantages compared to natural apidecine peptides: (i) longer half-life in mammal serum owing to higher protease stability, (ii) high antimicrobial activity with respect to one or more bacterial strains, particularly human pathogens or fungi, or other microbial infections, (iii) demonstrate a wide range of antimicrobial activity, (iv) cause slower development of resistance in microorganisms and (v) are not toxic for human cells, including erythrocytes.

EFFECT: improved properties of peptides.

21 cl, 8 dwg, 7 tbl, 6 ex

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