Synergistic combination including glyphosate and one of dcoit or oit or bbit

FIELD: chemistry.

SUBSTANCE: group of invention relates to a synergistic antimicrobial composition which includes a zinc salt of glyphosate and 4,5-dichloro-2-n-octyl-4-isothiazolin-3-one, and is intended for controlling the level of fungi and algae. The group of inventions also provides a method of inhibiting growth or controlling growth of microorganisms in building material by adding said synergistic antimicrobial composition, a coating composition containing said synergistic antimicrobial composition and a dry film made from said coating composition.

EFFECT: use of the group of inventions increases efficiency of inhibiting growth of microorganisms, provides more precise control of the growth of microorganisms without affecting health and/or the environment.

12 cl, 7 tbl

 

The present invention offers a combination of biocides, which are characterized by unexpectedly higher activity compared with the use of both individual antimicrobial compounds.

The use of combinations of at least two antimicrobial compounds can expand the potential markets, to reduce the working concentration and cost, and reduce waste. In some cases, commercial antimicrobial compounds do not effectively control the growth of microorganisms, even at high working concentration due to their low activity against certain types of microorganisms, such as microorganisms resistant to certain antimicrobial compounds. A combination of different antimicrobial compounds in some cases are used to provide overall control growth of microorganisms in a particular environment. For example, in the application WO 1998/121962 described the combination of 3-iodine-2-propynylbutylcarbamate and pyrithione zinc, but in this application are not expected to use any of the combinations claimed in the present invention. Moreover, there is a need for additional combinations of antimicrobial compounds that have relatively low impact on the health and/or the environment.

The aim of the present invention is to develo the TKA such additional combinations of antimicrobial compounds.

In some cases, antimicrobial compounds include liquid compositions for coatings, which are applied to the substrate and into a dry film. It is necessary that such dry film provided level control of algae and fungi on the surface and had minimal adverse effects on health and the environment.

Below is a description of the essence of the present invention.

The first object of the present invention offers a synergistic antimicrobial composition containing a glyphosate and one of the following compounds: 4,5-dichloro-2-n-octyl-4-isothiazolin-3-one (DHOT) or 2-n-octyl-4-isothiazolin-3-one (OIT), or N-butyl-1,2-benzisothiazolin-3-one (BBIT). The second object of the present invention proposes a method of inhibiting the growth of or controlling the growth of microorganisms in the construction material adding a synergistic antimicrobial composition according to the first object of the present invention. The third object of the present invention features a composition for coating containing a synergistic antimicrobial composition according to the first object of the present invention.

The fourth object of the present invention offers a dry film obtained from the composition for coating according to a third object of the present invention.

Below p is the fractional description of the invention.

In this context, used the following terms, definitions are given below, unless otherwise indicated.

The term "antimicrobial compound" means a compound capable to inhibit the growth of or controlling the growth of microorganisms, antimicrobial compounds include bactericidal, bacteriostatic, fungicidal, fungistatic, algaecide and legistations drugs depending on the magnitude of the dosage used, the condition of the system and the required level control of microorganisms. The term "microorganism" includes, for example, fungi (such as yeast and mold), bacteria, and algae.

In the description of the invention uses the following abbreviations: ppm million - mass part per million, ATSS American type culture collection and MIC - minimum inhibitory concentration. Unless otherwise stated, values are expressed in weight percents (wt.%). The percentage of antimicrobial compounds in the compositions of the present invention are defined in the calculation on the total weight of the active ingredients in the composition, i.e. themselves, antimicrobial compounds, excluding any quantities of solvents, carriers, dispersing agents, stabilizers, or other materials that may be present.

Used in this context, the term "DHOYT" means 4,5-dichloro-2-n-octyl-4-isot azolin-3-one, the term "should" denotes 2-n-octyl-4-isothiazolin-3-one, and the term "BIT" denotes N-butyl-1,2-benzisothiazolin-3-one. If in this context indicates that the ratio is X:1 or more, this indicates that the ratio is equal to Y l, where Y is equal to X or more, and if in this context indicates that the ratio is X:1 or less", it means that the ratio is equal to Z 1, where Z is equal to X or less.

Glyphosate refers to N-(phosphonomethyl)glycine (registration number 1071-83-6). Glyphosate is a known herbicide, which has been approved in many organizations for use in the control of weeds in crops food crops. It was found that one of the estimates is relatively low impact of glyphosate on the environment is its relatively low activity against various weeds. Some results characterizing the activity of glyphosate against various algae, is presented below:

Evaluation activity(1)Algae72 h96 hDay 7
EbC30green algae (Selenastrum capricornutum) 485 mg/lto 13.8 mg/l
EbC30green algae (Selenastrum capricornutum)460 mg/l
EC50marine algae (Skeletonema costatum)1.3 mg/l0.64 mg/l
EC50diatoms (Navicula pelliculosa)42 mg/l
EC50blue-green algae (Anabaena flos-aquae)15 mg/l

(1)Notes.

ECn/EC50- Effective concentration ECndenotes the concentration at which a specified period of time the compound has an effect on n% of the population. The value of EC50widely used, since it represents the most accurate reference point on the curve steps from concentration.

EbC50denotes the average effective concentration in terms of biomass (fogoros is she): the concentration, when the specified period of time, the increase in biomass is reduced by 50% compared with the control sample.

ErC50denotes the mean effective concentration in terms of speed of growth (algae): the concentration at which a specified period of time, the growth rate of algae cells is reduced by 50% compared with the control sample.

The above data indicate a relatively low influence of glyphosate on algae, indicating a relatively low effect of glyphosate on the environment. The above description is considered to be suitable for antimicrobial material that can be included in the composition for cover.

In the present invention proposes the use of glyphosate. Used in this context, the term "glyphosate" refers to glyphosate or salt of glyphosate. Salt of glyphosate denotes a salt of glyphosate and metal. Suitable metals include alkali metals, alkaline earth metals and transition metals. Salts of glyphosate are preferred compared to glyphosate. More preferred are salts of glyphosate and transition metals, the most preferred zinc salt of glyphosate.

Glyphosate is characterized by a relatively high solubility in water. Indicated relatively high solubility in water is not preferable for antimicrobial material, which can be included in the composition for coating or other building material, as dry coatings and building materials exposed to water that can wash the connection, characterized by a high solubility of the dry coating or building material. Preferred are salts of glyphosate, the solubility of which in water at 20°C is 3 g/l or less, more preferably 1 g/l or less, most preferably 0.3 g/l or less.

We can assume that salt of glyphosate have relatively low impact on health and the environment, as it is assumed that the action of salts of glyphosate on human health and the environment similar to the action of glyphosate.

The present invention features a composition that includes glyphosate and one of the following compounds: 4,5-dichloro-2-n-octyl-4-isothiazolin-3-one or 2-n-octyl-4-isothiazolin-3-one, or N-butyl-1,2-benzisothiazolin-3-one. Unexpectedly, it was found that this composition is characterized by the synergistic action as an effective biocide. First of all, it has been unexpectedly found that compositions which include a zinc salt of glyphosate and one of the following compounds: DHOYT or the RTO or BIT, characterized by the synergistic action as an effective biocide. Preferred ablauts the composition, which include glyphosate and DHOYT. Even more unexpectedly, it was found that compositions which include a zinc salt of glyphosate and DHOYT, characterized by the synergistic action as effective biocides.

If there is DHOYT, preferably the mass ratio of DHOYT/glyphosate 0.33:1 or more. If there is DHOYT, preferably the mass ratio of DHOYT/glyphosate is 2:1 or less. If the RTO, preferably the mass ratio of the RTO/glyphosate is from 0.3:1 to 0.4:1. If there is BIT, preferably the mass ratio of BIT/glyphosate is from 0.1:1 to 10:1.

If the composition includes more than one of the listed compounds isothiazolinone (i.e. DHOYT, Oita and BBIT), usually take into account the sum of masses of all these compounds isothiazolinone (i.e. the sum of: mass DHOYT (if present) + mass Oita (if present) + mass BIT (if present)). The preferred ratio of the sum of the masses of all these compounds isothiazolinone/mass of glyphosate ranging from 0.1:1 to 10:1.

A mixture containing glyphosate and one of the following compounds: DHOYT, or RTO, or BIT, can be included in the composition for coating. Glyphosate and one of the following compounds: DHOYT, or RTO, or BIT can be added to the composition for coating separately, the sludge is in the form of a mixture, or any combination of these compounds. Preferred compositions for coatings are liquid. Compositions for coatings are water or no water. Aqueous compositions for coatings containing 40 wt.% or more of water based on the weight of the composition for cover.

Among the embodiments of the present invention, in which glyphosate and one of the following compounds: DHOYT, or RTO, or BIT included in the paint composition or a different composition for coating, the preferred compositions for coatings are liquid compositions, especially compositions which contain dispersions of polymers in aqueous media.

In addition to inks and other compositions for coatings, combinations of biocides of the present invention is primarily suitable for the conservation of building materials, such as adhesives, sealants, seam sealants, insulation, upholstery cardboard and the like, polymers, plastics, synthetic and natural rubber, paper products, fiberglass, insulation, exterior insulation systems, roofing material and construction of cardboard, plaster, brick, mortar, plasterboard, timber and wood-plastic composite materials. If the combination of biocides of the present invention is present in the building material, it is preferable that some to whom Binali or all combination of biocides were on the surface of the construction material or close enough to the surface of the construction material, to suppress the growth of microorganisms on a given surface.

In some embodiments, use latex paint or other liquid compositions for coatings that contain combinations of biocides described in this context.

Compositions for coatings are designed for easy application of a layer of the composition for coating on a substrate and then drying or curing in air for drying, forming a dry film. Compositions for coatings contain a binder. Binders contain one or more of the following compounds: one or more polymers, one or more oligomers and/or one or more monomers. Oligomers and monomers in the binder composition intended for the polymerization and/or crosslinking during formation or after formation of the dry film. The polymers in the composition of the binder is not necessarily intended for stitching in the formation or after formation of the dry film.

Compositions for coatings optionally contain one or more dyes. The colorant is an inorganic or organic compound in the form of small solid particles. Dyes provide full or partial opacity of the dry film.

Combinations of suitable biocides for preservation of the dry film coating obtained after application of paint or other liquid compositions for covered who I am. Preferably the antimicrobial composition is an aqueous latex containing one or more combinations of biocides described in this context, or dry film coating, obtained by applying the paint to the surface. Water latex paint is a liquid aqueous composition for coating in which the binder polymer is in the form of a latex (i.e. in the form dispersed in water polymer particles). Preferred aqueous latex paint in which the binder contains one or more acrylic polymers.

Typically, the number of combinations of biocides of the present invention, providing control of microbial growth, is from 100 ppm million to 10000 ppm million of active ingredient. Preferably the amount of active ingredients in the composition is at least 300 ppm million, preferably at least 500 ppm million, preferably at least 600 ppm million, preferably at least 700 ppm million Preferably the amount of active ingredients in the composition is not more than 8000 ppm million, preferably not more than 6000 frequent./million, preferably not more than 5000 ppm million, preferably not more than 4000 ppm million, preferably not more than 3000 ppm million, preferably not more than 2500 ppm million, preferably not more than 2000 ppm mn, CR is doctitle not more than 1800 ppm million, preferably not more than 1600 ppm million above the concentration specified for liquid compositions comprising combinations of biocides, the content of biocides in dry film coatings is a higher value.

In the present invention it is also proposed a method of preventing microbial growth in building materials, especially in the dry film coatings, when included in the composition of the materials of any of the claimed combinations of biocides.

Typically, the antimicrobial composition is used to inhibit the growth of algae and/or fungi.

The composition according to the present invention includes glyphosate and one of the following compounds: DHOYT, or RTO, or BIT. It is assumed that in some embodiments, the composition includes one or more additional antimicrobial compounds.

It is assumed that the zinc salt of glyphosate is characterized by unexpected synergistic antimicrobial activity compared with the results expected for the combination of zinc and glifosato acid.

Below are examples of the implementation of the present invention.

Zinc salt of glyphosate was prepared as follows.

First pre-prepared reagents. Glifosato acid (15-20 g) were dried in an oven at 80-90°C during the night. Prepared 1 M solution of NaOH in deionized in the E.

The solution of glyphosate were prepared as follows. Deionized water (200 ml) measured in the beaker 600 ml containing magnetic Mielnik. Glifosato acid (15 g) was slowly added to a glass of water while stirring on the magnetic stirrer at moderate speed. For dissolution of glyphosate temperature was increased to 60-70°C, the temperature was monitored with a thermometer. pH is brought to 6.0 by adding 1 M NaOH solution. Glyphosate was dissolved when the pH value is approximately 2.4. The mixture was stirred for 5-10 minutes

The solution of the zinc salt was prepared as follows. Deionized water (100 ml) measured in the beaker with a volume of 400 ml, containing magnetic Mielnik. Zinc chloride (36,25 g, chemically pure, ≥98%, of the firm Sigma-Aldrich, catalog number 208086) was weighed and was slowly added to the water in a measuring Cup with stirring on a magnetic stirrer. The solution was heated to 65°C.

The solution of the zinc salt of glyphosate prepared as follows. A solution of zinc chloride was added to a solution of glyphosate using a Pasteur pipette (9 inches). After each addition of a small amount of a solution of zinc chloride in the solution of glyphosate before adding the next portion of the solution of zinc chloride precipitate was dissolved. When the precipitate did not dissolve, the pH was brought to 5. The remaining solution of zinc chloride was poured into the solution glyfo the ATA and the mixture was stirred overnight.

Note: control the pH of the solution is extremely important to obtain the desired product. Upon receipt of the reagents described in this context, it is necessary to ensure that the pH does not exceed 5.

Zinc salt of glyphosate was filtered as follows.

Collecting device for filtering comprising a Buechner funnel and flask Bunsen, United through the seal and is connected to a water-jet pump. The funnel was placed a filter paper (No. 41 firms Whatman™). To create a vacuum included pump and filter paper were filled with deionized water. Suspension zinc salt of glyphosate was slowly poured on filter paper and then washed once hot (about 50°C) deionized water and twice with isopropyl alcohol.

The final solution of the zinc salt of glyphosate prepared as follows.

The filter paper containing the precipitate of zinc salt of glyphosate, were removed and placed in a large bowl of percenage glass. The Cup was covered with a metal foil and the foil is pierced with several holes for ventilation. The precipitate was dried in an oven at 80-90°C during the night. Then the sediment was weighed into a clean pre-weighed glass vial with a label and record the mass of sediment.

Samples for the determination of antimicrobial active is ti was prepared as follows.

One biocide, or a mixture of biocides were added white acrylic latex paint that does not contain biocides, to receive the maximum total concentration of the investigated active ingredient/ingredients. Then the specified paint was diluted acrylic latex paint that does not contain biocides, to receive required for analysis of concentration. The total concentration of biocides changed depending on the type of the investigated mixtures of biocides in the range from 400 ppm million up to 3300 ppm million After adding or dilution of biocides each sample was stirred by hand for at least 1 min to form a homogeneous mixture. Each of the samples of paint, as control sample (not containing biocides)was used to obtain films on the black plastic panels of a copolymer of vinyl chloride and vinyl acetate (the Leneta company, Mahwah, NJ) using angular applicator (0,0762 mm, 3 mil). The panel carefully dried for at least 2 days, avoiding direct sunlight. From each panel cut out square disks (0.5 inch2, 1.27 cm2) and was used as substrate to determine the efficacy against fungi and algae. The specified size of the sample is allowed to surround the study disk agar by placing the disk sample in the hole analytical tablet. The analysis of each of which were acquired spent in two repetitions.

The analysis was performed under the following conditions.

To support the growth of microorganisms used in the appropriate environment (Bold's 3N for Chlorophyta, BG-11 for cyanobacteria and PDA for fungi). The investigated plates kept at room temperature (25-26°C) using the light / dark cycle for four weeks for algae. Records for the test fungi was kept at 30°C for four weeks. After completion of the incubation period was determined by the sample surface covered with visible gains microorganisms, in percent.

Seed algae

OrganismsAbbreviated nameTypeEnvironment analysis
Gloeocapsa sp.GsATCC 29159Unicellular, colony-forming cyanobacteriaBG-11
Oscillatoria sp.OsATCC 29135Filamentous cyanobacteriaBG-11
Nostoc communeNc CCAP 1453/29Unicellular, Chlorophyta CenobialBold
Trentepohlia aurea + Trentepohlia odorataTa + ToUTEX LB 429 + CCAP 483/4Filiform ChlorophytaBold
Chlorella sp. UTEX + Chlorella kessleriCs + CkATCC 30582 + ATCC 11468Unicellular chlorophyteBold
Calothrix parientinaCpUTEX LB 1952Filamentous cyanobacteriaBold

Seed fungi

OrganismsAbbreviated nameNo. ATCCMedia for cultivation and analysis
Aspergillus nigerAn9642PDA
Penicillium funiculosumPf11797PDA
Cladosporium herbarumC 11281PDA
Aureobasidium pullulansAp9348PDA
Trichoderma virideTv32630PDA
Alternaria alternataAa20084PDA

Determination of the effectiveness against algae was carried out as follows (modified method ASTM 5589).

Method ASTM 5589 is a standard accelerated method of analysis to determine the resistance of different coatings (including paints) damage to the algae. For performance analysis the sample size was reduced from Petri dishes to 6-well plates. One sample was placed with sterile forceps into the center of the agar layer (on the surface) painted side up. Seed algae were obtained by mixing equal concentrations (approximately 1×106CFU/ml) and equal volumes (depending on the number of samples, intended for planting) organisms with similar growth conditions.

In the study of synergism as inoculum for analysis received three mixed pool algae: a mixture of qi is nobacteria Gloeocapsa sp. and Oscillatoria sp., cultivated in the medium BG-11, single-celled Chlorophyta - Chlorella sp., Chlorella kessleri and Nostoc commune, which was mixed and cultured in medium Bold, filamentous algae Trentepohlia aurea, Tretepohlia odorata and Calotrix parientina, which were mixed and cultured in medium Bold.

To each well containing the sample, contributed seed, 400 μl of a mixture of organisms (approximately 1×106CFU/ml), providing uniform coverage of the entire surface (layer of paint and the surrounding agar) seeds. The tablets are kept at room temperature (25-26°C) using cycles of light (model OTT-Lite No. OTL4012P, 40 W, 26 kilolumen (KLM)) and dark for four weeks. At the end of each week was determined by the total covered area in percentage increments of 5%.

Determination of the effectiveness against fungi was carried out as follows (modified method ASTM 5590).

Method ASTM 5590 is a standard accelerated method of analysis to determine the resistance of different coatings (including paints) to damage by fungi. For performance analysis the sample size was reduced from Petri dishes to 6-well plates. For analysis at the bottom of each well of a sterile 6-hole tablet was placed a layer of agar. One sample was placed with sterile forceps into the center of the agar layer (on the surface) colored storona is up. Seed fungi were obtained by mixing equal concentrations (approximately 1×106CFU/ml) and equal volumes (depending on the number of samples for analysis), organisms with similar growth conditions. In the study of synergism as inoculum for analysis received three pool mixed mushrooms. Cladosporium herbarum was mixed with Trichoderma viride, Aspergillus niger was mixed with Penicillium funiculosum, a Alternaria alternata was mixed with Aureobasidium pullulans. To each well was made seed, 400 μl of a mixture of organisms (approximately 1×106CFU/ml), so that the entire surface (layer of paint and the surrounding agar) was uniformly coated seeds. The tablets are kept at a temperature of 30°C in the presence of moisture within four weeks. At the end of each week was determined by the total covered area in percentage increments of 5%.

The synergy index (IP) was calculated as follows.

IP was calculated by the method described in the article Kull F.C. and others, Applied Microbiology, v.9 (1961). In this study the IP was calculated by the formula below, and the minimum inhibitory concentration was determined by the inhibition (in %)that were recorded for individual biocide in respect of each of the investigated organism.

IP=Qa/QA+Q/QB,

where Qa denotes the concentration of A biocide in the mixture;

QA denotes the concentration of A biocide in quality is TBE only biocide;

Q denotes the concentration of biocide B in the mix;

QB denotes the concentration of biocide B as the sole biocide.

In this formula the value of IP<1 indicates synergism mixed biocides.

Note. If any active connection when the maximum investigated concentrations showed no overwhelming effect, this maximum concentration was used to calculate the estimated IP, and the sign "less than" ( < ) indicates that to achieve the desired level of suppression required a higher concentration of active compounds.

"OK" indicates the absence of the final values of the investigated concentrations, which satisfies the criterion of the percentage of suppression, prescribed for the calculation of each IP.

Listed below are compositions that contain zinc salt of glyphosate and one of the following compounds: DHOYT, or RTO, or BUT presented as examples of implementing the present invention. Other compositions are presented for comparison.

The results of the analysis of the zinc salt of glyphosate and glifosato acid is shown below.

Active connectionConc., part./millionInhibition (%) against different IP is legovanych organisms
Cs + Ck + NcCp + To + TaGs + OsAA + ArAn + PfTv + Ch
Zinc salt of glyphosate750100015402050
1500100072,547,527,575
2500100090703090
Glifosato acid75037,50157,502,5
150060 1072,520550
2500807,59067,51072,5
Control sample000000

The results of the analysis of the compositions of the zinc salt of glyphosate and DHOYT below.

Total concentration, part./million
AaTvAnPfApChCp + To + TaCs + Ck + NcGs + Os
Zinc salt of glyphosate/DHOYT, 1:1
Total concentration, part./million1725 300575300300300
Suppression, %80904010080100
IP2,050,500,590,630,630,55
Zinc salt of glyphosate/DHOYT, 1:2
Total concentration, part./million222222444222222444
Suppression, %60,080701008070
IP 0,260,310,550,440,440,71
Zinc salt of glyphosate/DHOYT, 1:5
Total concentration, part./million6906906906906901035
Suppression, %8080708010060
IP0,810,78<1,031,271,281,42
Zinc salt of glyphosate/DHOYT, 1:10
632,5632,5632,5949949949
Suppression, %609090806070
IP0,740,64<1,021,711,711,21
Zinc salt of glyphosate/DHOYT, 3:1
Total concentration, part./million412412825412412412
Suppression, %70809080100 100
IP0,490,84<a 0.270,930,930,87
Zinc salt of glyphosate/DHOYT,5:1
Total concentration, part./million3848251650384384384
Suppression, %80907080100100
IP0,461,790,890,880,880,84
Zinc salt of glyphosate/DHOYT, 10:1
Total concentration, part./million825825825825825825
Suppression, %808070100100100
IP1,001,880,361,951.951,91
Zinc salt of glyphosate
Total concentration, part./million825412,53300412,5412,5412,5
Suppression, %4080 0808080
DHOYT
Total concentration, part./million287,51150575575575825,5
Suppression, %408040802060

When the ratio of zinc salt of glyphosate/DHOYT from 1:10 to 10:1 synergism is observed. When the ratio of zinc salt of glyphosate/DHOYT from 0.33:1 to 2:1 synergism is observed in a wide range.

The results of the analysis of the compositions of the zinc salt of glyphosate and Oita below.

AaTvAnPfApChCp + To + TaCs + Ck + Nc Gs + Os
Zinc salt of glyphosate/Oita, 1:1
Total concentration, part./million33003300OK16502475825
Suppression, %4040808580
IP<4,174,172,61of 4.380,96
Zinc salt of glyphosate/Oita, 1:2
Total concentration, part./million27006751350 135020251350
Suppression, %60,06040808080
IP<4,271,07<1,241,763,141,55
Zinc salt of glyphosate/Oita, 1:5
Total concentration, part./million21602160OK54021602160
Suppression, %60408010040
IP<4,114,11 0,552,872,44
Zinc salt of glyphosate/Oita, 1:10
Total concentration, part./million19801485OK19801980OK
Suppression, %5040100100
IP<4,053.04 from1,772,44
Zinc salt of glyphosate/Oita, 3:1
Total concentration, part./million 825825OK3300412412
Suppression, %5540608580
IP<0,650,656,610,860,49
Zinc salt of glyphosate/Oita, 5:1
Total concentration, part./million33001650OK33001650825
Suppression, %5040708590
The <to 2.061,037,07of 3.640.99
Zinc salt of glyphosate/Oita, 10:1
Total concentration, part./millionOK3300OK33001650825
Suppression, %50807080
IP1,587,493,800,99
Zinc salt of glyphosate
Total concentration, part./million330016503300412,5412,5825
Suppression, %0400808080
DHOYT
Total concentration, part./million4504509001350900900
Suppression, %10050408010080

The results of the analysis of the compositions of the zinc salt of glyphosate and BIT below.

Cp + To + Ta + Cs + Ck + Nc
Zinc salt of glyphosate/BIT, 3:1
Total concentration, part./million2475
Suppression, %0
IP<1,0
Zinc salt of glyphosate/BIT, 1:10
Total concentration, part./million1485
Suppression, %0
IP<1,04
Zinc salt of glyphosate/BIT, 1:10
Total concentration, part./million990
Suppression, %0
IP<0,69
Zinc salt of glyphosate/BIT, 1:5
Total concentration, part./million1620
Suppression, %0
IP <0,69
Zinc salt of glyphosate/BIT, 1:1
Total concentration, part./million1650
Suppression, %0
IP<0,86
Zinc salt of glyphosate
Total concentration, part./million3300
Suppression, %100, OK
Total concentration, part./million1350
Suppression, %0

When the ratio of zinc salt of glyphosate/BIT from 1:1 to 3:1 and 1:5 and 1:10 synergism is observed. Three additional separate analysis was performed using mixtures of algae: Gs + Os, fast-growing fungi: An, Aa, Pf, Tv, and slow-growing fungi: Ap+Ch. When conducting these analyses, a synergistic effect was observed.

1. A synergistic antimicrobial composition in an effective amount to provide level control of algae and fungi, including lnkoval of glyphosate and 4,5-dichloro-2-n-octyl-4-isothiazolin-3-one, where the mass ratio of the zinc salt of glyphosate/4,5-dichloro-2-n-octyl-4-isothiazolin-3-one is from 1:10 to 10:1.

2. The method of suppressing the growth of or controlling the growth of microorganisms in the construction material, and the method includes a step of adding the specified construction material synergistic antimicrobial composition according to claim 1.

3. Composition for coating comprising a synergistic antimicrobial composition according to claim 1.

4. Dry film obtained by the method, which is that the layer of the composition for coating according to claim 3 is applied to the substrate and dried specified composition for coating or withstand the specified composition for coating on the air for drying.

5. A synergistic antimicrobial composition in an effective amount to provide level control of algae and fungi, including zinc salt of glyphosate and 2-n-octyl-4-isothiazolin-3-one, where the mass ratio of the zinc salt of glyphosate/2-n-octyl-4-isothiazolin-3 is 3:1.

6. A synergistic antimicrobial composition in an effective amount to provide level control of algae and fungi, including zinc salt of glyphosate and N-butyl-1,2-benzisothiazolin-3-one, where the mass ratio of the zinc salt of glyphosate/N-butyl-1,2-benzisothiazolin-3-one is from 1:1 to 1:10.

7. The method of suppressing the growth or to the control of microbial growth in building materials, and this method includes a step of adding the specified construction material synergistic antimicrobial composition according to claim 5.

8. Composition for coating comprising a synergistic antimicrobial composition according to claim 5.

9. Dry film obtained by the method, which is that the layer of the composition for the coating of claim 8 is applied to the substrate and dried specified composition for coating or withstand the specified composition for coating on the air for drying.

10. The method of suppressing the growth of or controlling the growth of microorganisms in the construction material, and the method includes a step of adding the specified construction material synergistic antimicrobial composition according to claim 6.

11. Composition for coating comprising a synergistic antimicrobial composition according to claim 6.

12. Dry film obtained by the method, which is that the layer of the composition for coating according to claim 11 is applied to the substrate and dried specified composition for coating or withstand the specified composition for coating on the air for drying.



 

Same patents:

FIELD: medicine.

SUBSTANCE: tableted antimicrobial composition contains polyhexamethylene guanidine hydrochloride (PHMH) in a combination with at least one ingredient specified in a group: quaternary ammonium compound (QAC), N,N-bis-(3-aminopropyl)dodecylamine and persol, as well as with an alkaline metal iodide and ammonium iodide. The antimicrobial composition has the following ratio of ingredients: PHMH - 10 to 70 wt %; QAC, N,N-bis(3-aminopropyl)dodecylamine and/or persol - 1 to 30 wt %; an alkaline metal iodide and ammonium iodide - 0.1 to 20 wt %.

EFFECT: using the invention provides the fast-acting tableted antimicrobial composition having a wide antimicrobial spectrum and a higher level of biocidal activity.

5 cl, 2 tbl, 2 ex

FIELD: agriculture.

SUBSTANCE: invention relates to the field of poultry industry and is intended for sanation of poultry hatching eggs. The method comprises processing the egg surface with aqueous solution of preparation based on milk whey with lactulose and quaternary ammonium compound trimethyloctadecyl ammonium bromide. As the aqueous solution of the preparation based on milk whey with lactulose and quaternary ammonium compound trimethyloctadecyl ammonium bromide 0.1-0.2% aqueous solution of the preparation is used, taken in equal proportions and diluted with water in a ratio of 1:10. Hatching eggs are processed 2-4 hours prior to their laying for incubation.

EFFECT: use of claimed method enables to carry out effectively sanation of poultry hatching eggs.

1 tbl, 1 ex

FIELD: cosmetology.

SUBSTANCE: agent comprising ethanol, glycerol, Carbopol ultrese and softened water according to the invention comprises aminomethylpropanol, essential oil selected from the group: eucalyptus essential oil, lemon essential oil, peppermint essential oil, anise essential oil, jasmine essential oil with the following ratio of components in g per 100 g of gel: ethanol 65.0-66.0; glycerol 1.0-2.0; Carbopol ultrese 0.16-0.18; aminomethylpropanol 0.06-0.08; essential oil 0.005-0.03; softened water - up to 100.0.

EFFECT: invention has a high antimicrobial activity against pathogenic bacteria, fungi and viruses and provides safety for life and health of people without causing allergy.

4 cl, 4 ex

FIELD: chemistry.

SUBSTANCE: biocidal composition for soaking napkins contains benzethonium chloride, sodium hydroxymethylglycinate, glycerine, decyl polyglucose, niacinamide, liposentol hydro, phenylmethanol and distilled water. Components are used in a defined ratio given in wt %.

EFFECT: invention improves biocidal properties of the napkin soaking composition.

3 tbl, 1 ex

Disinfectant // 2514097

FIELD: chemistry.

SUBSTANCE: invention discloses a disinfectant and can be used in prevention and treatment facilities, at first aid facilities, disinfection facilities, child care centres, public catering facilities, public utilities, transportation, veterinary, agriculture, food industry enterprises and other sectors, as well as for pre-sterilisation cleaning of medical items, including surgical and dental instruments, rigid and flexible endoscopes and other instruments. The disinfectant contains catamine AB, isobornyl acetate, isopropanol, α-pinene, glutaric aldehyde, glycerine, a nonionic surfactant and water in defined ratios.

EFFECT: disinfectant does not cause corrosion in articles made of metal and decomposition of metals and endoscope units; does not cause organic contamination.

3 tbl

FIELD: medicine, pharmaceutics.

SUBSTANCE: group of inventions refers to medicine, namely to clinical allergology, and may be used for extracorporeal allergen denaturation. That is ensured by treating a surface of objects with an allergen-denaturing composition. The composition contains calcium chloride or calcium acetate and lanthanum chloride or lanthanum nitrate in certain proportions.

EFFECT: combinations of the above metal salts selected in certain proportions provide the effective allergen denaturation ex vivo by a synergistic denaturation action of the given ingredients.

9 cl, 4 tbl

FIELD: medicine.

SUBSTANCE: synergistic antimicrobial composition contains a zinc salt of glyphosate and thiabendazole, wherein the weight ratio of thiabendazole to the zinc salt of glyphosate is from 0.1:1 to 10:1. A method of inhibiting the growth or controlling the growth of microorganisms in a construction material involves the step of adding the synergistic antimicrobial composition. A coating composition comprises the given synergistic antimicrobial composition. A dry film is made by a process comprising applying a layer of the synergistic composition to a substrate and drying.

EFFECT: controlling the growth of microorganisms, fungi, algae on the finish surfaces of the construction materials.

6 cl

FIELD: chemistry.

SUBSTANCE: described is a method of producing a highly purified disinfectant which contains polyhexamethylene guanidine hydrochloride, characterised by that flush water from production of polyhexamethylene guanidine hydrochloride in solid form is used. The flush water is mixed with 25% NaCl solution in ratio of 1:1 (polyhexamethylene guanidine hydrochloride solution with impurities: NaCl solution) while stirring constantly for 1 hour at temperature in the range of 50-60°C. Stirring is stopped and the mixture is cooled to 5°C, thereby dividing the mixture into two parts; the bottom part is drained, neutralised and sent for recycling and the top part containing up to 60-70% polyhexamethylene guanidine hydrochloride is further diluted to 50% with an aqueous solution of quaternary ammonium salts (QAS) to QAS concentration of not higher than 5%.

EFFECT: extracting highly purified, concentrated high-molecular weight polyhexamethylene guanidine hydrochloride from flush water, reducing the amount and toxicity of waste water, improving disinfecting properties and increasing output of the commercial-grade product when producing polyhexamethylene guanidine hydrochloride in solid form, reducing cost.

1 cl, 1 ex, 2 tbl

FIELD: medicine.

SUBSTANCE: invention refers to disinfection. The experimental method for the elimination of pathogenic and opportunistic microorganisms is characterized by the fact that a mixture containing the pathogenic and opportunistic microorganisms are processed with a disinfection composition which is metal oxyhydrate gel prepared by alkaline metal chloride deposition by 2% ammonia in a min. 5-litre reactor; fresh metal oxyhydrate 20-10-6 m3 is placed in an electrochemical cells with graphite electrodes of a charged cluster metal particle generator; what is added is a bacterial solution of the medium of 105 microbial cells in 1 ml dissolved in distilled water 10 ml; the medium is exposed to polarized currents of the cluster oxyhydrate particles of metal oxyhydrate gel for 2 to 6 hours; metal oxyhydrate gels are prepared by using metal chloride salts presented by zirconium or iron chloride salts with the electrode distance no more than 70 mm.

EFFECT: invention provides the more effective inactivation of pathogenic and opportunistic microorganisms that are infectious agents in human and animals.

7 tbl, 2 dwg, 6 ex

Antiviral agent // 2496522

FIELD: medicine, pharmaceutics.

SUBSTANCE: group of inventions discloses an antiviral agent (versions) able to inactive a virus. The antiviral agent characterised by containing the basic substance, cuprous microparticles, and inorganic microparticles to fix the cuprous microparticles on the basic substance. The inorganic microparticles are coupled together by chemical bonds formed between silane monomers found on the surface, and each of the inorganic microparticles is coupled with the basic substance by a chemical bond between silane monomer and the basic substance for the purpose of forming the areas wherein the above cuprous microparticles are expected to be accumulated.

EFFECT: group of inventions provides high antiviral activity.

16 cl, 3 tbl, 7 ex

FIELD: medicine.

SUBSTANCE: synergistic antimicrobial composition contains a zinc salt of glyphosate and thiabendazole, wherein the weight ratio of thiabendazole to the zinc salt of glyphosate is from 0.1:1 to 10:1. A method of inhibiting the growth or controlling the growth of microorganisms in a construction material involves the step of adding the synergistic antimicrobial composition. A coating composition comprises the given synergistic antimicrobial composition. A dry film is made by a process comprising applying a layer of the synergistic composition to a substrate and drying.

EFFECT: controlling the growth of microorganisms, fungi, algae on the finish surfaces of the construction materials.

6 cl

FIELD: medicine.

SUBSTANCE: invention refers to disinfection. The experimental method for the elimination of pathogenic and opportunistic microorganisms is characterized by the fact that a mixture containing the pathogenic and opportunistic microorganisms are processed with a disinfection composition which is metal oxyhydrate gel prepared by alkaline metal chloride deposition by 2% ammonia in a min. 5-litre reactor; fresh metal oxyhydrate 20-10-6 m3 is placed in an electrochemical cells with graphite electrodes of a charged cluster metal particle generator; what is added is a bacterial solution of the medium of 105 microbial cells in 1 ml dissolved in distilled water 10 ml; the medium is exposed to polarized currents of the cluster oxyhydrate particles of metal oxyhydrate gel for 2 to 6 hours; metal oxyhydrate gels are prepared by using metal chloride salts presented by zirconium or iron chloride salts with the electrode distance no more than 70 mm.

EFFECT: invention provides the more effective inactivation of pathogenic and opportunistic microorganisms that are infectious agents in human and animals.

7 tbl, 2 dwg, 6 ex

Antiviral agent // 2496522

FIELD: medicine, pharmaceutics.

SUBSTANCE: group of inventions discloses an antiviral agent (versions) able to inactive a virus. The antiviral agent characterised by containing the basic substance, cuprous microparticles, and inorganic microparticles to fix the cuprous microparticles on the basic substance. The inorganic microparticles are coupled together by chemical bonds formed between silane monomers found on the surface, and each of the inorganic microparticles is coupled with the basic substance by a chemical bond between silane monomer and the basic substance for the purpose of forming the areas wherein the above cuprous microparticles are expected to be accumulated.

EFFECT: group of inventions provides high antiviral activity.

16 cl, 3 tbl, 7 ex

FIELD: medicine, pharmaceutics.

SUBSTANCE: invention refers to disinfection. The composition contains hydrogen peroxide and an aqueous solution of copper or one of its derivatives, such as copper salts, as well as at least one salt of an anionic surfactant inhibiting decomposition of hydrogen peroxide in the presence of copper. The composition may be prepared immediately before use by mixing the aqueous solution of copper and the aqueous solution of hydrogen peroxide.

EFFECT: invention provides simultaneous disinfection and decontamination of the objects infected by special infectious agents (NCTA), such as prions, and common infectious agents (CTA), such as bacteria, yeast, viruses, mould spores, mycobacteria, bacterial spores.

23 cl, 5 tbl

FIELD: medicine.

SUBSTANCE: invention refers to public health, veterinary science, food processing, pharmaceutical and biotechnological productions. Disinfection objects are processed by the system of disinfectants consisting of at least two preparations, wherein at least one of the preparations is specified in a group of oxidants, namely oxygen-chlorine substances, and at least one of the preparations is specified in a group of non-oxidants, including, QACs - quaternary ammonium compounds, tertiary amines, aldehydes, guanidines. The system of disinfectants is packed within a package so that each preparation is placed separately, and each following preparation is used only after the previous preparation has been consumed completely.

EFFECT: invention provides the controlled rotation of disinfectants that prevents the developing acquired microorganism resistance.

3 ex

FIELD: medicine, pharmaceutics.

SUBSTANCE: invention refers to medicine, particularly to stabilised aqueous antimicrobial compositions. The antimicrobial composition contains 2-25 wt % of 2,2-dibrom-3-nitrilopropionamide, 5-30 wt % of an aliphatic compound containing 2 to 6 hydroxyl groups, 20-70 wt % of ethylene glycol, 2-30 wt % of water.

EFFECT: invention provides stability and low viscosity of the composition.

10 cl, 5 ex

Disinfectant // 2481126

FIELD: medicine, pharmaceutics.

SUBSTANCE: invention refers to medical microbiology and medicine, namely to disinfection, and may be used for preparing disinfectants for disinfection of the surfaces contaminated by microorganisms, medical, sanitary and microbiological equipment. The disinfectant contains hydrogen peroxide, a water suspension of silver nanoparticles and non-ionic detergent Triton X-100 in certain amounts.

EFFECT: invention provides improved microbicidal activity on vegetative and spore forms of microorganisms at the low concentrations of its ingredients.

4 dwg, 3 ex

Disinfectant // 2481126

FIELD: medicine, pharmaceutics.

SUBSTANCE: invention refers to medical microbiology and medicine, namely to disinfection, and may be used for preparing disinfectants for disinfection of the surfaces contaminated by microorganisms, medical, sanitary and microbiological equipment. The disinfectant contains hydrogen peroxide, a water suspension of silver nanoparticles and non-ionic detergent Triton X-100 in certain amounts.

EFFECT: invention provides improved microbicidal activity on vegetative and spore forms of microorganisms at the low concentrations of its ingredients.

4 dwg, 3 ex

FIELD: chemistry.

SUBSTANCE: group of inventions relates to disinfection and sterilisation. The acidic liquid composition used is a liquid composition which contains (a) an acidic oxidant, (b) nitric acid or salt thereof and (c) at least one carboxylic acid selected from a group consisting of monocarboxylic acids, dicarboxylic acids and tricarboxylic acids, or salt thereof. In the disinfection and sterilisation method, the acidic liquid composition contains an acidic oxidant having an excellent corrosion inhibiting property (property of preventing rust, property of inhibiting formation of an oxide film) for aluminium-based metals even in acidic conditions, without affecting the appearance or function of such metals.

EFFECT: efficiently used to disinfect or sterilise an object having a part made from aluminium-based metals.

12 cl, 20 tbl, 4 ex

FIELD: medicine.

SUBSTANCE: invention relates to disinfectants. An antiseptic disinfectant contains the film-forming preparation iodopyrone containing 6-8% of active iodine and water, contains ethanol in the following proportions, wt %: iodopyrone - 1.0-1.3 (per active iodine basis), ethanol - 20, water - the rest. The antiseptic disinfectant represents a ready-to-use preparation presented in the form of a solution or a spray.

EFFECT: invention provides the qualitative and quantitative stability of the active substance in the easily water-washable disinfectant which does not stain and has a non-irritant, prolonged effect If kept in an unopened container, it has a shelf-life of 2 years from the date of manufacture.

3 ex

FIELD: medicine, virology, pharmacy.

SUBSTANCE: invention relates to using derivatives of dithiocarbamate of the formula: R1R2NCS2H and oxidized forms of these compounds, in particular, their dimmers and their pharmaceutically compatible salts for preparing an agent used in treatment or prophylaxis of infection caused by RNA-containing viruses that damage respiratory tract and inducing disease. Also, invention relates to a disinfecting agent containing dithiocarbamate compound and a method for disinfection of surfaces, media and cell cultures.

EFFECT: valuable medicinal properties of compounds.

19 cl, 14 dwg, 14 ex

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