Hypoallergenic dermatological composition

FIELD: medicine, pharmaceutics.

SUBSTANCE: invention relates to chemical-pharmaceutical industry and deals with application of extract of above-ground part/parts of oats, excluding grains, containing from 2 to 15% of flavonoids and from 0.2 to 2% of A and B avenacosides, for preparation of cosmetic and dermatological composition.

EFFECT: invention represents dermatological and cosmetic composition, containing such extract and possessing hypoallergenic properties.

8 cl, 2 ex, 2 tbl, 3 dwg

 

The present invention relates to allergies to grains (cereals).

In the framework of the present invention cereals include family Roaseae (Cereals) and pseudospace.

The term "grains" refers to the family of Cereals, ripe grain (caryopsis) which are used in food, particularly due to the high content of starch (Paris R.R., Moyse N. Precis de matiere medicale. II Pharmacognosie speciale. Spermaphytes (continued): Angiospermes. Monocotyledones Dicotyledones. Apetales et Diapetales. 2ndEdition. Paris: Masson, 1981, p.16). The term "cereal" as a rule, refers to the seeds of such plants.

In botany grasses belong to the family Roaseae or Gramineae.

These grasses usually are herbaceous monocotyledonous plants with hollow stems between nodes (the Solomina by), bearing arranged in two rows of leaves are often split and ligularias the leaf sheath. The flowers are located in the secondary spikelets and often grouped in a compound spike or clusters called panicles". Flower reduced to stamens and ovaries: two stipules or two glumes protect the secondary spike, and two bottom floral scales, one of which is a front, inclined wrapping, often shetinkonogie, together with the formation of the flower. Three protruding stamens vary, attached to the back. The ovary has a single carpel with two op is Chenin pistils, directly adjacent to the ovule and developing each individual fruit called a caryopsis; abundant endosperm is starch.

This family includes about 700 genera and 12,000 species.

A large part of the cereal grasses used for food purposes (wheat, barley, rye, oats, sorghum, millet, maize, rice, etc).

The species most commonly used cereals are presented below in Table 1.

Table 1
The main species consumed cereals, belonging to the family Roaseae
Latin nameLocal name
Avena abyssinica Hochst.
Avena brevis Roth
Avena nuda LThe naked oats
Avena sativa L
Bromus mango E.Desv.
Coix lacryma-jobi var. ma-yuen (Rom. Caill.) Stapf
Dendrocalamus strictus (Roxb.) Nees
Digitaria exilis (Kippist) Stapf
Digitaha iburua Stapf
Echinochloa esculenta (A. Braun) H. ScholzBarnyard grass plushie millet
Eleusine coracana subsp.coracanaWeed styptic
Eragrostis tef (Zuccagni) TrotterPolovicka
Hordeum vulgare subsp. vulgareBarley ordinary multi-row
Oryza glaberrima Steud.African rice
Oryza sativa LRice planting
Panicum hirticaule J. Presl
Panicum miliaceum subsp. miliaceumMillet present
Panicum sumatrense Roth
Paspalum scrobiculatum L.
Pennisetum glaucum (L.) R. Br.Pearl millet (pearl millet)
Phalaris canariensis L.The Canary bird
Secale cereale subsp.cerealeThe rye crop
Secale strictum subsp. africanum (Stapf) K. Hammer
Setaria italica subsp. italicaMillet Italian, spickle Italian, sorghum ordinary
Setaria palmifolia (J. Koenig) Stapf
Setaria pumila subsp. pumilaSpickle small
Sorghum bicolor subsp. bicolorSorghum bicolor sorghum ordinary
×Triticosecale spp.Triticale
Triticum aestivum subsp. aestivumSoft wheat spring wheat
Triticum aestivum subsp. spelta (L.) Thell.Wheat culture (spelt)
Triticum aestivum subsp. sphaerococcum (Percival) Mackey
Triticum monococcum subsp. monococcumThe wheat anothername cultural, wheat anothername (spelt)
Triticum timopheevii subsp. timopheevii
Triticum turgidum subsp. carthlicum (Nevski) A. Love &D. LoveWheat Persian
Triticum turgidum subsp. durum (Desf.) Husn.Durum wheat
Triticum turgidum subsp. polonicum (L.) Thell. Wheat Polish
Triticum zhukovskyi A. M. Menabde & Eritzjan
Urochloa deflexa (Schumach.) H. Scholz
Urochloa ramosa (L.) T. Q. Nguyen
Zizania palustris L.
Zea mays subsp.mays LCorn sugar

Some crops that are members of other Botanical families, similar to cereals and called pseudoplane. Examples of these types relating to cereals, but not part of the family Roaseae, which are also used and can cause food allergies, include species listed below in Table 2.

Table 2
Most commonly consumed species related to crops, but not belonging to the family Roaseae
Latin nameLocal nameFamily
Amaranthus caudatus L.Cattail fire red
amaranth grain
amaranth tailed
Alopecurus
Amaranthaceae
Amaranthus cruentus LAmaranthus hybrid droopingAmaranthaceae
Amaranthus hypochondriacus LAmaranthaceae
Chenopodium album L.Pigweed whiteChenopodiaceae
Chenopodium berlandieri subsp. nuttalliae (Saff.) H.D. Wilson & HeiserChenopodiaceae
Chenopodium pallidicaule AellenChenopodiaceae
Chenopodium quinoa subsp. quinoaQuinoa quinoa
Peruvian rice
Chenopodiaceae
Fagopyrum esculentum MoenchBuckwheat sown
Buckwheat true
Polygonaceae
Fagopyrum tataricum (L.) Gaertn.Buckwheat TatarPolygonaceae
Helianthus annuus LThe sunflower is an annualAsteraceae
Sesamum indicum LSesamePedaliaceae

Cereals and pseudolocal contain a large number of be the Cove: structural proteins, biologically active proteins and reserve proteins. Osborne (1907) classified them according to their solubility:

is a water - soluble albumins,

- globulins, soluble in physiological solutions

the prolamins, soluble in water and ethanol

- glutamine, insoluble in these solvents, partially soluble in solutions of urea or guanidine.

Examples:

WheatOatsBarleyCorn
AlbuminLeucosin
GlobulinsDestinyAdenaline
ProlaminsThe gliadinsThe gliadinsGordiniSeine
GlutelinGluteninEveniniHordenineTheanine

In contrast, the protein content in the aboveground parts, in particular sheets, consists mainly of:

- membrane proteins of the chloroplast (proteins, often related to lipids and pigments),

soluble proteins of the chloroplast (stroma): RuBPCase.

The leaves of the quinoa quinoa, sunflower, oats, barley, and Zizania palustris, young Wheatgrass and sprouts sesame seeds, rice, wheat and maize are used for cosmetic purposes. For example, young green shoots of barley used as a means of slowing the aging process.

In addition, in the prior art disclosed:

- the extract of the leaves of amaranth (Amaranthus), used as anti-inflammatory drugs, in particular in the treatment of atopic dermatitis (patent document JP 2000143524),

the composition containing the extract, oat straw extract and rose-tea for the treatment of inflammatory reactions of the skin for local use (EP 1474159),

- cosmetic composition which protects against oxidation using oil rice germ (EP 1704898),

- cosmetic composition comprising an extract of wheat germ (WO 9526183),

- cosmetic product that has a regenerating effect on the skin, containing extract of stems of sunflower (FR 2487674). Flour from cereals, in particular wheat flour, as you know, when inhaled causes is stmu from flour and grain dust, and rhinitis. The strong IgE response was observed in the case of albumin and globulin proteins which are soluble in neutral solutions. However, some studies have also demonstrated the reactivity of proteins, soluble in ethanol or acidic environment (glutenins and gliadins). Recently immunoblot study of the blood serum of patients allergic to flour and grain dust showed that the major allergens in neutral fractions of wheat and barley flour refer to low molecular weight range (about 20 kDa).

The consumption of cereals sometimes leads to celiac disease and herpes dermatitis. Some studies have identified the involved antigen related to glutinosae fraction soluble in ethanol proteins.

Despite the importance of cereals in the human diet suggest that they lead to the deterioration of atopic dermatitis, particularly in children. Similarly, immunological research (essentially, SPT test scratch test and RAST - radioallergosorbent test) lead to the identification of proteins involved in wheat, oats, rye, barley, rice, millet and maize and demonstrate the cross-reaction between wheat and barley, or rye (Varjonen E, Vainio E, Kalimo K, Juntunen-Backman K, Savolainen J Skin-prick test and RAST responses to cereals in children with atopic dermatitis. Characterization of IgE-binding components in wheat and oats b an immunoblotting method (Responses scratch tests and RAST on cereals in children with atopic dermatitis. Characterization de-binding components in wheat and oats by the method of Western blot turns) Clin Exp Allergy 1995, 25:1100-1107) (Palosuo K, Alenius H, Varjonen E, Kalkkinen N, Reunala T. Rye γ-70 and γ-35 secalins and barley γ-3 hordein cross-react with go-5 gliadin, a major allergen in wheat-dependent, exercise-induced anaphylaxis (Cross-reaction of γ-70 and γ-35 secalinum rye and hordein γ-3 barley with ω-5 gliadin, a major allergen caused wheat anafilaktichesky reactions physical stress). Clin Exp Allergy, 2001, 31:466-473). It is assumed that when absorbed into the grains are the cause of atopic dermatitis, involved the least soluble proteins (gliadins and glutenin). Many preliminary studies show that in the case of children with atopic dermatitis significant role of food hypersensitivity, and removal of food allergens leads to clinical improvement.

Reported some manifestation of contact Allergy to cosmetic products based on extracts of cereals (especially wheat protein, whether it be hydrolyzed or non-hydrolyzed, and sesame) in people with hypersensitivity. It has been shown that the hydrolysis of the protein of wheat, which could be a solution to alleviate allergies, is on the contrary much more sensitizing (increasing sensitivity), structural changes caused by hydrolysis, identify new places is binding antigen (Pecquet S, Lauriere M New allergens in hydrolysates of wheat proteins (New allergens in protein hydrolysates of wheat). Rev Fr Allerg Clin Immunol 2003, 43:21-23).

It is believed that people with allergies should avoid proteins of cereals as oral, and the local application, this applies in particular to children.

Thus, there is a need and strong demand for cosmetic products designed for people with hypersensitive or allergic to grains.

Unexpectedly and surprisingly the inventors have found that a cosmetic composition comprising the extract of the aerial parts of cereals or pseudolocal collected preferably before the appearance of the ear, does not lead to cross-reactions with the proteins that are responsible for allergies to grains.

Thus, the aim of the present invention is the provision of an extract of the aerial part (s) of cereal and/or pseudosasa, with the exception of grains, for use by people with allergies to protein grain.

The term aerial parts of cereal and/or pseudosasa in this context refers to any portion, located above ground level, except for the beans.

Preferably, the "above-ground parts of cereals and/or pseudolocal" consisted of leaves and/or stems and/or secondary spikelets, and/or flowers, excluding grains.

Before occhialino, to the extract according to the invention represented extract of the aerial part (s) of cereal and/or pseudosasa collected before the appearance of the ear.

In the context of the present invention, the phrase "aerial parts of the herb and/or pseudosasa collected until spike" refers to the aerial parts of the herb or pseudosasa harvested after seed germination (after approximately 2 weeks to 2 months after germination) during the stage of elongation up to, but excluding it, the appearance of the ear.

In the context of the present invention, the term "elongation" refers to the growth phase, the corresponding growth of the stem length and the entry in the phase of earing, before flowering.

Preferably, the extract according to the invention is obtained from the aerial part (parts) of wheat, rice, barley, or oats, more preferably oats.

Extract of the aerial part (s) of oats, mainly used according to the invention by people who are allergic to grain protein, contains from 2 to 15% flavonoids and from 0.2 to 2% of avenacosides A and B, preferably from 5 to 10% flavonoids isovitexin-2"-O-arabinopyranoside and isoorientin-2"-O-arabinopyranoside and/or less than 1 ppm (ppm) (ppm) protein, preferably less than 0.5 ppm and even more preferably less than 0.3 ppm of proteins.

Used extract of the aerial parts) oats differs by the presence of the target flavonoid and saponin. The last analyzed by high-performance liquid chromatography, using one of two different techniques that are appropriate for each type of connection.

The number of these different molecules varies depending on the extraction conditions. The major flavonoids are isovitexin-2"-O-arabinopyranoside and isoorientin-2"-O-arabinopyranoside. The main saponins are avenacosides a and B. This bisdesmosides steroid saponins.

Preferably, the extract according to the invention is an extract of the aerial part (s) of wheat, rice or oats, collected before the advent of the ear, and preferably, the above-ground part (s) of oats harvested before the appearance of the ear.

Preferably, the extract according to the invention is an extract obtained in an organic solvent.

The extract according to the invention can be prepared by extraction with an organic solvent of the above-ground part (s) of cereal or pseudosasa (approximately 2 weeks to 2 months after germination during the growth stage up to, and excluding, the appearance of the ear) after drying and grinding. In particular, use of an organic solvent selected from the group consisting of ketones, esters, C1-C4-alcohols and their mixtures in any mixing ratio. Preferably the organic solvent, selected from the group consisting of acetone, methyl ethyl ketone, isobutyl ketone, ethyl acetate, C1-C4-alcohol and their mixtures in any mixing ratio.

The extraction is preferably carried out under stirring or statically.

The extraction is carried out at boiling under reflux or at room temperature.

Preferably, the extraction is carried out at a ratio plant/solvent in the range from 1:7 to 1:20, preferably from 1:8 to 1:12.

Preferably, the extraction is carried out for from 30 minutes to 48 hours, most preferably for 60 to 120 minutes. The extraction may be repeated 2 or 3 times.

The cake obtained in the extraction step, and then separated from the extract by centrifugation or filtration, after which the solution may be more or less concentrated to obtain dry mass.

The bleach processing may be carried out either by degreasing with concentration, precipitation and filtration, or by adding to a concentrated or non-concentrated extracting solution absorbent substrate, such as activated carbon or absorptive resin.

At the stage of drying may be added to the substrate in a weight ratio relative extractable dry matter in the range from 1 to 75%. The substrate may Ave is dostavljati a sugar, such as maltodextrin, lactose, silicon dioxide or any other kosmetologicheskii acceptable substrate.

Preferably, the extract was obtained by extraction with acetone or a mixture of acetone/water containing up to 20% water. Acetone or a water-acetone containing 20% water, the extract contains the target molecule, flavonoids and saponins and largely depleted proteins. In fact, the methods of analysis for the determination of proteins, are described in the European Pharmacopoeia, is inconclusive and electrophoretic migration during electrophoresis in polyacrylamide gel in the presence of sodium dodecyl sulfate after precipitation from acetone and symptoms with the help of Kumasi blue or silver nitrate shows no bands, indicating the presence of protein. The application of a control sample in the same assay allows to evaluate the detection limit of the protein, as appropriate, about 1 ng, and, thus, the protein content in the extract is less than 1 ppm (based on the amount of extract applied to the gel). Preferably, the specified protein content was less than 0.5 ppm, more preferably less than 0.3 ppm of proteins.

Another objective of the present invention is the use of the extract of the aerial part (s) of cereals and/or pseudolocal, IP the connection grains, people who are allergic to protein grain.

Another objective of the present invention refers to the use of the extract of the aerial part (s) of cereals and/or pseudolocal, with the exception of grains, for the preparation of hypoallergenic dermatological compositions, i.e. compositions that minimizes and/or prevents risks of allergic reactions in people who are allergic to protein grain.

Another objective of the present invention refers to the use of hypoallergenic cosmetic composition containing the extract of the aerial part (s) of cereals and/or pseudolocal, people who are allergic to protein grain.

Dermatological or cosmetic composition used according to the invention may, in particular, contain additives and auxiliary facilities for the preparation of dosage forms, such as emulsifying agents, thickeners, geleobrazovanie, water neutralizers, lyophilizers additives, stabilizers, colorants, fragrances and preservatives.

Dermatological or cosmetic composition used according to the invention, also includes traditional dermatologically compatible fillers.

It can be prepared in the form of emulsion, water-in-oil (W/O) or oil-in-water (O/W), heterogeneous emulsion, such as, for example,emulsion water-in-oil-in-water (W/O/W) or emulsion oil-in-water-in-oil (O/W/O), or in the form of an aqueous dispersion or oil dispersion, gel or aerosol.

Dermatologically compatible fillers can be any fillers known to the person skilled in the art suitable for obtaining a composition intended for topical application in the form of a cream, lotion, gel, ointment, emulsion, microemulsion, means to spray, shampoo and the like.

Preferably, dermatological or cosmetic composition used according to the invention contain from 0.1 to 10 wt.% the extract of the aerial part (s) of cereal or pseudosasa, with the exception of grains, by weight of the total composition. Preferably, this composition includes an extract of the aerial part (s) of the cereal according to the invention in quantities of from 0.1 to 5 wt.% of the total weight of the composition.

A brief description of graphic materials

Figure 1: Comparison of profiles of total protein from the seeds and young shoots of oats by electrophoresis in polyacrylamide gel with sodium dodecyl sulfate (a 12.5% polyacrylamide gel) in the presence of sodium dodecyl sulfate in the manifestation of using Kumasi blue.

Figure 2: Western blotting, staining enzymes with polyclonal antibodies against proteins of the aerial parts.

Figure 3: Western blotting, staining enzymes with polyclonal who antibodies against proteins of grains. The following examples illustrate the invention without limiting it.

Example 1: Preparation of extract of the aerial parts of oats harvested before the appearance of the ear, extraction with acetone

400 kg of dried aerial parts of oats harvested before the appearance of the ear, grind and then placed in the reactor 10 by volume mixture of acetone/water with stirring for 1 hour at room temperature.

After phase separation solid/liquid receive the first extract. The cake is extracted again with 10 volumes of a mixture of acetone/water for 1 hour at room temperature with stirring. After separation of solid/liquid phases receive a second extract and combine it with the first. The resulting solution is concentrated to a water content of 1.33.kg, and then filtered. Thus obtained extract is dried using a microwave after adding maltodextrin substrate (25%/native extract, a sufficient number (qsp)).

In this way receive 36 kg powder pale brown color, containing 6% flavonoids (isovitexin-2"-O-arabinopyranoside and isoorientin-2"-O-arabinopyranoside), 0.3% avenacosides A and 0.6% avenacosides B, while the protein content in the thus obtained extract is less than 0.3 ppm

Example 2: Biochemical and immunological comparison of extract grain cereal and extract of the aerial hour is it the same cereal, collected before the appearance of the ear.

No cross reactions between extracts of cereals and extracts of the aerial parts was demonstrated by biochemical (extraction and determination of protein) and enzyme immunoassay methods (specific detection of the presence of the protein through the formation of antibodies).

Extract grain protein can be prepared as follows.

Grain milled and extracted with sodium hydroxide at pH 8. After filtration, the filtrate is precipitated by adding hydrochloric acid to pH 5.4. After centrifugation the precipitate is taken, placed in aqueous solution and cialiswhat against water in a dialysis bag with the switch-off of 6-8 kDa, at a temperature of 4°C during the night.

Extract of the aerial parts can be prepared as follows.

Fresh material:

- extraction by solubilization in Tris.HCl buffer solution with a pH of 8.0, filtered and the subsequent series of two sediment from acetone to remove salts,

or:

- extraction by solubilization in pampering buffer laemmli's method at pH 7.5, containing urea and thiourea, filtration and subsequent series of two sediment from acetone to remove salts.

Of dry material:

extraction for 27 hours by solubilization in pampering buffer laemmli's method at pH 7.5, coderamasino and thiourea, filtration and subsequent series of two sediment from acetone to remove salts.

Biochemical and immunological study

Biochemical methods

I. Electrophoresis

Principle of operation: a specific Method for studying protein is polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate in the presence of sodium dodecyl sulfate negatively charged proteins move in polyacrylamide gel in the direction of the cathode based only on their mass, protein is determined using dyes such as Kumasi blue or silver nitrate.

Used positive control corresponds either to extract the total protein in the aerial parts of oats before the appearance of the ear, or the extract total protein grains obtained as described above.

II. Illustrative results - 1

Comparison of profiles of total protein grain and aerial parts of oats before the appearance of the ear using polyacrylamide gel electrophoresis (a 12.5% polyacrylamide gel) in the presence of sodium dodecyl sulfate in the manifestation of using Kumasi blue.

The electrophoresis profile, shown in figure 1, reveals a significant difference in the protein composition of both the studied extracts, particularly in relation to core proteins. However, the only way to ensure it is the quality difference between the two series of proteins, is the use of immunological methods for the specific detection of antigen-antibody.

Immunochemical method

This method requires, first of all, obtaining antibodies against all proteins in the aboveground parts or beans. To this end, the extracts of all the proteins of the aerial parts and seeds of the same species are introduced into the organism rabbits. Then from the blood serum of animals are polyclonal antibodies.

The aim is to demonstrate that proteins aerial parts of oats until spike proteins differ from grains and that there is no cross-reaction between proteins of grains and proteins aboveground parts.

Antibodies directed against proteins of the aerial parts of oats before the appearance of the ear, in comparison with proteins of grains of oats,

Antibodies directed against proteins of grains of oats, in comparison with proteins aerial parts of oats before the appearance of the ear,

using the methods of ELISA (enzyme-linked immunosorbent assay) and Western blot.

I. Direct ELISA

ELISA (enzyme-linked immunosorbent assay) is an immunochemical method for determining the presence of antigen in solution. The principle consists in the fixation of proteins in the test solution in the well of the tablet with the subsequent processing antebellum polyclonal antibodies. Next, the second antibody anti-lgG rabbit, United with the enzyme, show the t after washing, the presence of the antigen in the well of the tablet using a simple colorimetric reaction.

II. Western blotting

Principle: the Method of Western blotting consists of polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate protein mixture followed by staining using the specific interaction between antigen-antibody. This will be identified only those proteins that are detected using polyclonal antibodies. The first antibody is then detected with a second antibody (in this context antibody anti-lgG rabbit), coupled with an enzyme such as alkaline phosphatase.

Illustrative result 2 and 3

Cross-reactions between proteins of grain and antibodies directed against proteins of the aerial parts of oats before the appearance of the ear (Figure 2), and cross-reactions between proteins aerial parts of oats until spike and antibodies directed against proteins of the grain (Figure 3).

Method Western blotting confirmed the first results obtained by ELISA, namely, that the proteins of the aerial parts of oats until spike proteins differ from grain and cross-reaction between them is missing.

The patient is immunized against protein grain oats with diet, will not, therefore, respond to the extract of the aerial parts (except grains), even if it contains proteins.

1. Application of the extract of the aerial parts/parts oats, excluded who eat grains, containing from 2 to 15% flavonoids and from 0.2 to 2% of avenacosides A and B, for use in food.

2. The use according to claim 1, in which the aboveground part/parts oats collected before the appearance of the ear.

3. The use according to claim 1 or 2, wherein the extract contains from 5 to 10% flavonoids isovitexin-2"-O-arabinopyranoside and isoorientin-2"-O-arabinopyranoside.

4. The use according to claim 1 or 2, wherein the extract contains less than 1 ppm of proteins.

5. Application of the extract of the aerial part/parts of oats, with the exception of grains, which contains from 2 to 15% flavonoids and from 0.2 to 2% of avenacosides A and B, for the preparation of hypoallergenic cosmetic compositions.

6. Application of the extract of the aerial parts/parts oats, except grains containing from 2 to 15% flavonoids and from 0.2 to 2% of avenacosides A and B, for the preparation of hypoallergenic dermatological composition.

7. Dermatological composition possessing hypoallergenic properties, containing as active ingredient an extract of the aerial parts/parts oats, except grains containing from 2 to 15% flavonoids and from 0.2 to 2% of avenacosides A and B.

8. Cosmetic composition having allergenic properties containing the extract of the aerial parts/parts oats, except grains containing from 2 to 15% flavonoids and from 0.2 to 2% of avenacosides A and B.



 

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FIELD: medicine, pharmaceutics.

SUBSTANCE: invention relates to the pharmaceutical industry and represents application of a composition, containing Bifidobacterium breve CNCM I-3865 (NCC2950) for preparation of a composition for prevention of allergic diarrhoea.

EFFECT: invention provides extension of an arsenal of means for prevention of allergic diarrhea.

7 cl, 1 dwg

FIELD: medicine, pharmaceutics.

SUBSTANCE: invention relates to chemical-pharmaceutical industry, particularly to method of treatment of dermatological allergic state. Proposed method comprises injection of efficient amount of [4-(5-aminomethyl-2-fluorophenyl)piperidine-1-silt][7-fluorine-1-(2-metoxyethyl)-4-trifluorometoxy-1H-indol-3-il]methanon or its appropriate N-oxide, pharmaceutically acceptable salt or solvate.

EFFECT: higher efficiency.

5 cl, 1 ex, 1 tbl

FIELD: chemistry.

SUBSTANCE: invention relates to an agent for preventing and/or treating an allergic disease selected from pollinosis, allergic rhinitis, allergic conjunctivitis, atopic dermatitis and asthma, which is a low-molecular polysulphated hyaluronic acid derivative.

EFFECT: obtaining a low-molecular polysulphated hyaluronic acid derivative.

15 cl, 103 dwg, 17 tbl, 55 ex

FIELD: medicine, pharmaceutics.

SUBSTANCE: invention refers to pharmaceutics and medicine and concerns preparing a fast-acting effective and safe agent for treating rhinitis. Solving the problem provides the agent for treating rhinitis, particularly allergi rhinitis containing C-type natriuretic peptide (CNP) and/or B-type natriuretic peptide (BNP) as an active ingredient.

EFFECT: invention provides the notable health improvement in rhinitis, particularly in allergic rhinitis, and besides, the therapeutic effect, ensures fast and prolonged action, and gives no local side effects.

21 cl, 7 ex, 7 tbl

FIELD: medicine, pharmaceutics.

SUBSTANCE: what is presented is using a composition containing galactooligosaccharide, fructooligosaccharide and uronic acid oligosaccharide in preparing a composition for oral administration into an infant for preventing the local administration of corticosteroids and/or preventing the administration of a calcineurin inhibitor into the above infant, wherein uronic acid oligosaccharide represents a pectin degradation product and/or an alginate degradation product, and wherein using the corticosteroids and/or administering the calcineurin inhibitor is applicable for treating eczema, infantile eczema, atopic dermatitis, dermatitis herpetiformis, contact dermatitis, seborrheic dermatitis, neurodermatitis, psoriasis and intertrigo. Particularly, the composition is a nutritional composition.

EFFECT: what is shown is reducing probability of the local administration of corticosteroids and dermatological preparations to be required for the purpose of preventing the above skin diseases, or reducing the length of using the corticosteroids.

5 cl, 2 ex

FIELD: medicine, pharmaceutics.

SUBSTANCE: invention refers to naphthalene carboxamide derivatives of general formula I which possess the properties of protein kinase or histone deacetylase inhibitors. The compounds can find application for preparing a drug for treating inflammatory diseases, autoimmune diseases, oncological disease, diseases of the nervous system and neurodegenerative diseases, allergies, asthma, cardiovascular diseases and metabolic diseases or disease related to hormonal diseases. In general formula I: , Z represents CH or N; each of the groups R1, R2 and R3 represents hydrogen, halogen, alkyl, alkoxy or trifluoromethyl; R4 represents or X represents a benzene ring or a pyridine ring; R5 represents one or more substitutes specified in a group consisting of hydrogen, halogen, alkyl, alkoxy or trifluoromethyl. The invention also refers to a method for preparing the above compounds, a pharmaceutical preparation and using them.

EFFECT: preparing the compounds which possess the properties of protein kinase or histone deacetylase inhibitors.

13 cl, 10 tbl, 6 dwg

FIELD: medicine, pharmaceutics.

SUBSTANCE: invention refers to pharmaceutical industry and represents a complex of biologically active substance for treating allergic diseases of various genesis, characterised by the fact that it has been recovered from cod liver oil by gradual fractionation from ballast lipids by extraction in a two-phase oil and water extractant, centrifugation and ultrafiltration or diafiltration through a material with separation limit 25 kDa, and contains peptides 30-55%, amino acids 40-65%, carbohydrates 2-8%, micro and macroelements 2-13%.

EFFECT: invention provides the drug spectrum broadening.

8 ex, 3 tbl

FIELD: chemistry.

SUBSTANCE: invention relates to novel of 2,4-pyrimidine diamine compounds of formula I, which inhibit degranulation of immune cells and can be used in treating cell reactions mediated by FcεRI or FcγRl receptors. In formula (I) each R2 and R4 is independently phenyl substituted with one or more R8 groups or a heteroaryl selected from a group consisting of , where the heteroaryl is optionally substituted with one or more R8 groups and at least one of R2 and R4 is a heteroaryl; R5 is selected from a group consisting of (C1-C6)alkyl, optionally substituted with one or more identical or different R8 groups, -ORd, -SRd, fluorine, (C1-C3)halogenalkyloxy, (C1-C3)perhalogenalkyloxy, -NRcRc, (C1-C3)halogenalkyl, -CN, -NO2, -C(O)Rd, -C(O)ORd, -C(O)NRcRc, -C(NH)NRcRc, -OC(O)Rd, -OC(O)ORd, -OC(O)NRcRc; -OC(NH)NRcRc, - [NHC(O)]nORd, R35 is hydrogen or R8; each Y is independently selected from a group consisting of O, S and NH; each Y1 is independently selected from a group consisting of O, S and NH; each Y2 is independently selected from a group consisting of CH, CH2, S, N, NH and NR37. Other values of radicals are given in the claim.

EFFECT: improved efficiency.

19 cl, 6 tbl.

FIELD: medicine, pharmaceutics.

SUBSTANCE: invention refers to new 2-S-benzylpyrimidine derivatives having CRTH2 receptor antagonist activity. In formula 1: R1 means -CO2H; R4a and R4b mean hydrogen; W means -C(O)NR7-; R2 and R3, each independently mean F; Cl; Br;-NR10R11 or (C1-C6)alkoxy, optionally substituted by 1-3 halogen atoms; R5 means hydrogen; R6 means (C1-C6)alkyl; (C6-C19)aryl or (5-15)-member heteroaryl containing nitrogen, oxygen or sulphur atoms as heteroatoms, wherein above aryl and heteroaryl are optionally substituted by one or more substitutes specified in a group consisting of halogen; (C1-C6)alkyl optionally substituted by 1-3 halogen atoms; and (C1-C6)alkoxy optionally substituted by one, two or three halogen atoms; R7 means hydrogen; R10 and R11, each independently mean (C1-C6)alkyl; or R10 and R11, together with N, whereto attached form a 3-8- member saturated or unsaturated ring optionally containing one or more O or S atoms, or one or more additional N atoms in the ring; k is equal to 0; m is equal to 1.

EFFECT: invention also refers to using the above compounds for preparing a drug for treating allergic and inflammatory diseases mediated by CRTH2 receptor activity, such as asthma, atopic dermatitis, allergic conjunctivitis, Churg-Strauss disease, sinusitits, basophilic leukaemia, and recurrent urticaria.

27 cl

FIELD: medicine, pharmaceutics.

SUBSTANCE: invention refers to biotechnology and immunology. The preparation contains an antibody, histidine and Polysorbate 80. Besides, a method of treating a subject with using the above preparation and a method of stabilising anti-human α-interferon antibody 13H5 are described. The invention can be used in medicine.

EFFECT: what is disclosed is a stable aqueous preparation containing the antibody or fragment thereof which are specifically bound to human α-interferon.

7 cl, 3 tbl, 2 ex

FIELD: chemistry.

SUBSTANCE: invention relates to cosmetic industry and provides a composition which facilitates production of type I collagen in human dermal fibroblasts, which contains one or more compounds selected from a group consisting of D-aspartic acid, D-alanine and/or salts thereof, and one or more pharmaceutically acceptable additives.

EFFECT: invention widens the range of agents which facilitate production of type I collagen in human dermal fibroblasts.

4 cl, 6 dwg, 6 ex

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