Biodegradable wound coating and method for preparing biodegradable wound coating

FIELD: medicine.

SUBSTANCE: what is described is a coating in the form of a film which contains the following ingredients, wt %: low-molecular edible chitosan 5.3-5.7, glycerol 2.2-2.8, ceruloplasmin 0.06-0.08, L-asparaginic acid 0.04-0.06, a solvent with pH 5-7 - the rest. What is described is a method for preparing the coating consisting in the fact that a chitosan weigh is diluted in the solvent at 1000 mg of the weigh to 15 ml of the solvent, mixed and placed in a thermostat at a temperature of 37-42°C for 1-2 hours. The mixture is added with ceruloplasmin diluted in the solvent at 1:10 to form a homogenous hydrogel. L-asparaginic acid is diluted in the solvent pre-heated to 37-40°C and added to the mixture to provide pH of the mixture 5-7. A plasticising agent in the form of glycerol is added in the amount of 2.2-2.8% of total volume of the prepared biomass The prepared biomass is placed into containers to form a uniform layer of the coating 3-5 mm high and to form a film by drying the biomass for 18-24 hours in the thermostat at a temperature of 37-40°C.

EFFECT: wound coating provides the highest clinical effect.

6 cl, 6 dwg, 2 ex

 

The group of inventions relates to medicine, biology, veterinary medicine, pharmacology. These technical solutions can be used in the manufacture of wound dressing for the treatment of wounds of the skin of various origins.

Known wound dressings made in the form of sheets or films of a mixture of synthetic polymers and polymers of biological origin. Coverings made only of synthetic polymer component, such as polyurethane [patent SIEVE No. 2871218], or multi-component, for example from a mixture of polyurethane and polyallomer ether [the Federal Republic of Germany patent No. 34098558], they have good physical and mechanical properties, quite durable, waterproof and vapor-permeable, but only performs a protective function and do not have the ability to stimulate the regeneration process.

Also known wound dressings in the form of collagen sponges or bandages that are applied active substances. Known, in particular, porous sponges for the treatment of wounds of collagen [USSR author's certificate No. 561564]; mixtures of gelatin, chitosan and formaldehyde [patent PRC No. 1097980]; gelatin and formaldehyde with the addition of antibiotics [RF patent №2033149]; cellulose and chitosan [Japan's bid No. 0376029]; collagen and chitosan [PCT patent No. 8504413].

The main disadvantage of these sponges is relatively low wound healing is aktivnosti, due to sticky lips on the wound. Pure collagen sponges have poor supply of oxygen to the zone of repair.

We know quite a lot of samples of wound coverings, differing in chemical composition. From the current wide range of polymer wound coverings are all medico-biological requirements of the biodegradable coating of polysaccharides, which can be useful in the early stages of treatment of wounds and later. General properties of biocompatible materials from polysaccharides, including chitosan, are hydrophilic, causing high absorbing capacity (up to 5000%), good adhesion to the wound, the lack of toxicity and irritant, and hemostatic properties. Marked their stimulatory effect on the healing process of wounds and burns, which contributes to more rapid development of granulation tissue, accelerates epithelization. The presence in the wound of polysaccharide materials beneficial to the repair processes at all stages of treatment of wounds. The main problem of obtaining coatings of natural polysaccharides is to achieve a good mechanical strength of the coating and stability on the wound. Development of biodegradable polymer coatings with high sorbent is capable of being the stew and various terms resorption is currently the most current trends in the field of creation of effective biological dressings for the treatment of wounds. Coating of chitosan air and vapor permeable, prevent the invasion of microorganisms from the outside, create an optimal climate in the wound, promote cell growth and proliferation in the wound. Chitosan, in addition to stimulating proliferation in the early stages of wound healing, is very useful in the final phase of healing - the restructuring of the scar. His presence in the wound helps to avoid the formation of unsightly scars [RF patent №2468129].

Known for dressing, one option of which contains chitosan and made in the form of flexible perforated film [RF application No. 99100105].

However, this film is multi-layered and, consequently, impossible for her full congruence with the wound surface and the removal of wound by absorbing absorbing layer bandage that leads to the necessity of frequent change of dressings.

Also known biologically active sorbing polymer film, created on the basis of chitosan [kildeeva NR, Vikhoreva T.A., Larionov A.S., galbraikh PS // Modern approaches to the development of effective suture materials and polymer implants: Mater. III international. proc. M., 1998, 130-131 C.], which contains the enzymatic preparation trypsin and modified cross-linking reagent - sodium dodecyl sulfate, showing, in addition, antimicrobial properties.

However, the film is poorly modeled on the wound. Riskyproject bandages wet creates the potential for discharge of wound of the dressing in the wound. In the composition of the film is toxic to the human body, the reagent is sodium dodecyl sulphate.

Known polymer film on the wound based on chitosan, having a thickness of 5-50 μm, modified crosslinking agent epichlorohydrin, which may also contain 0-20% of polyvinyl alcohol or polyethylene glycol, and antibacterial or antiseptic substances [patent WO No. 2001/0141820].

However, this film has a relatively low adsorption capacity, rapid biodegradation of the film in the wound environment, which makes cleansing wounds, because adsorbed film of wound and products of biodegradation remain in the wound, the complete removal of the film is possible only when used on relatively dry wounds.

Known for dressing, representing a perforated film and containing chitosan in the form of salts of organic acids (acetic, succinic or glycolic acid), glutaric aldehyde, polyvinyl alcohol and biologically active additives in the form of CO2-extracts of medicinal plants [RF patent №2219954].

Disadvantages headbands: lack of congruence with the wound surface, low adsorption capacity, restricted permeability. In the case of use as biologically active additives CO2-extracts of medicinal plants, such as yarrow, about what Aichi, plantain, eucalyptus, wormwood, St. John's wort, coriander, increases the likelihood of allergic reactions. Disadvantages are also complex composition bandages, include toxic glutaraldehyde, low elastic properties due to the increased crosslinking between glutaraldehyde, chitosan and polyvinyl alcohol.

It is also known wound covering based on collagen-chitosan complex for repair of skin defects in the form of a sponge, a gel, a colloidal solution, film. Chitosan component contains chitosan with deacetylation of 0.95-0.99 and molecular weight of 100-1000 kDa in the form of ascorbate chitosan with ascorbic acid content of 1.8 g/l of dry chitosan and chondroitinase acid - 5-100 mg/g of dry chitosan, hyaluronic acid - 10-100 mg/g of dry chitosan, heparin - 2.5-5 mg/g of dry chitosan and whey growth factor cattle - 11-220 µg/g of dry chitosan [RF patent №2254145].

However, part of the wound cover whey growth factor cattle is a protein component providing additional allergenic effect.

The closest analogue to the claimed biodegradation wound coating is biodegradable film, which contains pectin, chitosan, water, odnokomntnuyu hydrochloric acid, PLA is typinator - glycerin and structure-former - three percent solution of methyl cellulose [RF patent №2458077].

However, this wound covering has its disadvantages. As is known, the addition of amendment (3% solution of methylcellulose) limits the possibility of the formation of films of different thickness, perimeter and, thus, creates some obstacles for a snug fit the film to the surface of the wound, thereby, reducing its adhesion to the wound surface. In addition, methylcellulose can cause mild allergic reactions that can lead to metabolic disorders, inhibiting regeneration of damaged tissues.

The second claimed in this application the invention is a method for biodegradable wound dressings.

A method of obtaining wound dressings "CHITOSKIN"described in the Russian patent for a utility model No. 8608. Sample of chitosan and collagen separately dissolved in acetic acid, then mixing the solutions, and expose the mixture to dialysis against distilled water with a periodic change of water up until pH valezuela mixture does not exceed 5.2, then add the biologically active substance and the amendment. The resulting solution was frozen in the freeze-drying chamber to -35°C and then subjected to freeze-drying.

The main nedostate the method of obtaining this wound dressings - a large percentage of defects in industrial production (70%). He expressed that during lyophilization is often observed plasma density sponge, consisting in compressing the sponge (reduction of the area of the sponge in the lyophilization up to 30% of the original) with the loss of the porous structure, as well as its spacelane and cracked with loss of flat shapes. As a result of these phenomena wound coating is hard, poorly simulates the wound surface. Apparently on the occurrence of the contraction of the jaws is strongly influenced by the rate of freezing of the polymer solution (gel). Used in industrial installations at the speed of freezing is greater than 4°/hour in the material there are temperature fluctuations, which provokes the formation of uneven patterns of long and non-equilibrium phase separation, resulting in further drying to the deformation of the plate material and loss of porosity.

Also known is a method of obtaining films based on chitosan, including the preparation of the molding mixture is air-dried sample of chitosan and solution of organic acid, coating the resulting solution on a substrate, followed by curing solution on the substrate before reaching the film structure. Forming solution comprises chitosan in the form of a salt of acetic or succinic acid. After keeping the film structure of the mod is functioning water vapor or an aqueous solution of 0.5 N. hydrochloric acid to the degree of swelling of the film 70-190%, while providing the relative elongation of the film at break of at least 20%. It also describes the way in which the film is placed in distilled water or physiological solution (0.9% NaCl) to the degree of swelling of the film is not less than 90%, while providing the relative elongation of the film at break of at least 30% [RF patent №2429022].

However, this method is time-consuming, has many sequential steps, including the preparation of the molding solution for 1 day with periodic shaking. Getting film is carried out in 2-3 days.

Closest to the claimed method for producing a biodegradable wound coverage is a method of obtaining a biodegradable film described in the patent of Russian Federation №2458077. Pectin is dissolved in distilled water. Then for better dissolution of the components of the solution are placed in a thermostat for 1 minute at a temperature of 37-38°C. the Chitosan dissolved in 1 BC (odnomomentnoe) hydrochloric acid. For better dissolution of the components of the solution are placed in a thermostat for 1 minute at a temperature of 37-38°C. Then the obtained solutions of pectin and chitosan preferably in equal proportions poured and stirred until complete dissolution of the formed clots. In order for the film was solid and evenly the CTD is alas from the substrate, after stirring clots in the resulting solution add the plasticizer and the amendment. The resulting mass is gently poured into a Petri dish. Film formed on the glass substrate (in a Petri dish) for 20-24 hours at a temperature of from 0 to 25°C.

However, the dissolution of chitosan in odnomomentnoe hydrochloric acid does not allow handling during manufacture of the film pH of the molding solution. It is known that the physiological pH of the coating allows to achieve optimal physiological conditions for the regeneration process.

Objective of the claimed invention regarding wound dressings is to increase its antioxidant properties, ensuring the physiological pH of the coating with the ease of use and low cost of its components.

Objective of the claimed invention regarding the method is a simplification of the process of obtaining wound dressings that do not require expensive equipment, ensuring maximum clinical effect when using cover.

The essence of the invention about the cover is that biodegradative wound covering, made in the form of a film containing chitosan, a plasticizer in the form of glycerol, the solvent further comprises ceruloplasmin and L-AC is Aracinovo acid in the following ratio of components, wt.%:

chitosan - 5,3÷5,7;

glycerin - 2,2÷2,8;

ceruloplasmin - 0,06÷0,08;

L-aspartic acid - 0,04÷0,06;

solvent - else

the use of chitosan of low molecular weight dietary chitosan, and a solvent with a pH of 5-7. States also biodegradative wound covering with the above described characteristics, in which the solvent used saline solution or distilled water. Moreover, there is also biodegradative wound covering with the above characteristics, which film has openings with a diameter of 0.2-0.5 mm and the concentration distribution in it 10-15 per 100 mm2.

The essence of the claimed invention regarding the method lies in the fact that in the production method of biodegradable wound dressings, including: sample preparation of chitosan; the last breeding in the solvent by adding a sample of chitosan in a solvent, mixing the resulting mixture and placing it in thermostat; adding to the mixture of plasticizer in the form of glycerol and placing the obtained biomass in the tank, ensuring the formation of a film by drying the biomass within 18-24 hours, the solvent used with pH 5-7, the addition of chitosan prepared from food low molecular weight chitosan and throw it in the solvent at the rate of 100 mg of sample in 15 ml of solvent, while the resulting mixture was placed in a thermostat at a temperature of 37-42°C for 1-2 hours, then the mixture was added diluted in solvent ceruloplasmin rate of 1:10 with ensuring the formation of homogeneous hydrogel, after dissolving L-aspartic acid in a pre-heated up to 37-40°C the solvent, providing the proportion of 100 mg L-aspartic acid in 10 ml of solvent, and added to the mixture to obtain a pH of the mixture 5-7; the plasticizer is introduced into a mixture of 2.2-2.8% of the total volume of the resulting biomass, the latter is stirred with a speed of 100-150 rpm within 30-60 seconds; the location of the biomass in the vessel is accomplished by providing education uniform coating layer with a height of 3-5 mm; drying the coating is performed in a thermostat at a temperature of 37-40°C.

Applying the method of obtaining biodegradable wound coverage with the above-described characteristics, in which the resulting film perforined with provision of hole diameter 0.2-0.5 mm and the concentration distribution 10-15 per 100 mm2.

The technical result of the claimed invention regarding coverage

It is known that in the wound, especially infected, report substantial metabolic disorders, including the activation of free radical oxidation products which additionally have a damaging de is the effect on the cell membrane. This leads to a slowdown of regenerative processes. This problem has been eliminated in the present invention is wound coverage by introducing into the composition of an antioxidant, such as ceruloplasmin, which reduces the level of toxic oxygen free radicals, thus contributing to the acceleration of regenerative processes.

Damage to the integrity of the skin lead to the development of a whole cascade of pathological reactions, and this, in turn, causes significant changes in the pH of biological liquids. In order to restore physiological levels of pH and stabilize them in the composition of the wound cover included sauterelle agent in the form of L-aspartic acid, in addition to actively affect the synthesis of DNA and RNA.

The use claimed in this application is the set of components developed by the authors in amounts to produce biodegradative wound covering having regenerating, antioxidant, sufficient antibacterial properties that prevent infection of wounds, with the wound cover quite flexible and provides the necessary adhesion to the wound surface.

Execution biodegradable elastic coating in film form, fully and reliably cover the wound surface, which does not require repeated on the Eseniya, repeating the spatial location of wound damage, you can also use it as a carrier for additional therapeutic components. The wound coated with the inventive composition and physical characteristics (elastic film) isolates the wound surface from penetration of pathogenic and conditionally pathogenic microflora, provides gas exchange and water balance, which is the most important factors to ensure the flow of the regeneration process. Use in the inventive biodegradation wound covering as the basis of chitosan eliminates the need for re-coating, the use of dressings and any additional impacts on the wound. Described in this application, the coating can be applied in a domestic environment, because chitosan and other components at their local application do not show toxic and allergic effects, so that the coating can be applied in all age groups of patients with different somatic status both in hospitals and at home. At the same time chitosan exhibits antibacterial and regenerative properties on the wound surface, creating optimal conditions for the activity of other components of the wound covering, providing a cumulative effect, p is Iisalmi activity of the source components.

In the composition of the inventive coatings are cheap components, produced in the territory of the Russian Federation, available in the open, over-the-counter access.

The technical result of the claimed invention regarding the method

The manufacture of wound dressings do not require the use of expensive equipment and special conditions, namely the homogenization of the starting components is due to the impact of temperature and uniform mixing. The end result is achieved by open drying under developed conditions, which allows to obtain ready-to-use wound coverage with maximum adhesion to the wound surface, plasticity, and hydrophobicity. Obtaining films with the given height and a uniform distribution of active components due to the sequence of the proposed method, temperature, time characteristics, and the presence of physical factors, such as mixing, merging components in normal atmospheric conditions, can achieve regenerative and preventive antibacterial properties against wounds of soft tissues. The use of the claimed coating requires no special skills and is available to the General population in everyday life.

The claimed group of inventions is illustrated using Fig, showing:

figure 1 - General view of the biodegradable wound dressings from her rough surface in contact with the wound;

figure 2 - General view of the biodegradable wound cover of a glossy surface;

figure 3 - biodegradative wound cover in a folded condition by illustrating its flexibility and plasticity;

figure 4 - General view of the biodegradable wound dressings on the biological object in 1 days after application;

figure 5 - General view of the biodegradable wound dressings on the biological object in 6 days after application;

figure 6 - General view of the biodegradable wound dressings on the biological object in 8 days after application.

Biodegradative wound covering is made in the form of a flexible translucent film. It consists of low molecular weight dietary chitosan, the plasticizer in the form of glycerol solvent, ceruloplasmin and L-aspartic acid. As the solvent used saline solution or distilled water with a pH of 5-7. The ratio of components in the film are as follows, wt.%: chitosan - 5,3÷5,7; glycerol - 2,2÷2,8; ceruloplasmin - 0,06÷0,08; L-aspartic acid - 0,04÷0,06; solvent - rest. In private cases, the film may have holes with a diameter of 0.2-0.5 mm with a concentration of their houses is adelene it 10-15 pieces 100 mm2.

A method of obtaining a wound cover is as follows.

Prepare a portion of the food of low molecular weight chitosan. Bred last in a solvent, in particular saline solution or distilled water, to which add cooked before addition of chitosan in the solvent, placed in containers, such as glass, made of polypropylene, at the rate of 1000 ml of sample to 15 ml of solvent. Stir the mixture and put it in a thermostat at a temperature of 37-42°C for 1-2 hours to ensure complete dissolution of chitosan in a solvent to form a translucent homogeneous mass of yellow-brown color. Then in a separate container bred ceruloplasmin in a solvent of 10 ml of ceruloplasmin in 1 ml of solvent. Divorced ceruloplasmin added to the mixture, ensuring the formation of homogeneous hydrogel. Then prepare the solvent of 10 ml, stirring it into thermostat to a temperature of 37-40°C. is Dissolved in a separate container in a heated solvent L-aspartic acid, providing a ratio of 100 mg L-aspartic acid in 10 ml of solvent. Add the prepared solution of L-aspartic acid in the mixture to obtain a pH of the mixture 5-7. After you add in the mixture of plasticizer in the form of gli is Wendy Erin of 2.2-2.8% of the total volume of the subsequently received biomass. Mix the past, for example, using a magnetic stirrer with a speed of 100-150 rpm for 30-60 seconds. Then distribute the biomass by longitudinally oriented containers, covering the entire surface of the latter with the formation of a uniform coating layer with a height of 3-5 mm In most cases for this as the tanks used Petri dishes made of polypropylene. Place these containers placed in them by the biomass in a thermostat at a temperature of 37-40°C for 18-24 hours prior to complete drying and the formation of a wound dressing in the form of films, having on one side a glossy surface, on the other hand rough.

In the future, give the film a form of RAS and placed them on the area of the pathological focus, drawing the rough surface inside the wound and insulating the entire wound surface from the external environment.

After the process is complete epithelialization in the presence of residual wound coating on the surface of the skin it carefully wash off with water. In the case of large wounds with copious exudation to improve the process of gas exchange and maintain optimal water balance in the damaged surface, necessary for the implementation of healing, film perforined with provision of hole diameter 0.2-0.5 mm and the concentration distribution 10-15 per 100 mm2.

Example 1.

The claimed drug b is l tested on 30 laboratory white rats-males weighing 160-220, The research was conducted in compliance with the research Protocol approved by the ethics Committee of SEI HPE "Saratov state medical University of the RF Ministry of health, - minutes No. 13 dated April 10, 2007 and is not contrary to the Geneva Convention of 1985 on the "International principles for biomedical research using animals."

All animals were simulated paleologou skin wound size 400 mm2. Why after pre-treatment of the skin, in aseptic conditions, anesthetized, shaved from wool in the interscapular region in rats was dissected skin with subcutaneous tissue in the form of a square 2×2 sm - 400 mm2the contour previously applied the stencil. Then laboratory animals were divided into two groups-experimental and comparison of 15 rats each.

In the comparison group, the treatment of the wound was performed by a single exposure on the wound surface isotonic sodium chloride solution and removal of necrotic tissue. In the experimental group after the same procedure on the wound surface has imposed a wound covering, completely covering the wound, taking his rough surface to the wound surface and lightly press it.

In the dynamics of the studied changes daily square RAS animals from the experimental group and the comparison group.

The daily reduction of the area of RAS at the Expo is imentally animals during treatment was %:

Day researchThe comparison groupExperienced group
34,6±1,210,8±0,8
p<0,001
5th12,4±2,133,1±0,5
p<0,001
7th16,4±0,524,2±1,7
p<0,001

Thus p is the confidence level differences in performance relative to the comparison group.

Complete wound healing was noted by the 10th day 7 animals of the experimental group, the 14-th day - the remaining 8 rats from the experimental group.

Throughout the experiment all animals were producing bacteriological examination of the wound surface. In all rats of the experimental group crops wound surface remained sterile during all periods of observation.

Wound healing in 11 animals in the comparison group were noted only on the 14th day. In 4 rats comparison group due to the contact of an open wound with conditionally pathogenic flora of the environment or native flora of the animal was noted wound infection 5 days E. coli - 2×105KOE. In 2 of them the animals were observed complete healing of wounds on the 21st day. 1-day in 2 other animals in the comparison group remained the same flora in the amount of 2×10 2SOME and the wound was cleansed of pathogens with the completion of the repair process only to the 26th day.

To assess the status of the processes of free radical oxidation was determined on the 14th day spectrophotometric biochemical parameters of blood of experimental animals: the content of malondialdehyde and activity of ceruloplasmin. The content of malondialdehyde in animals of the experimental group was statistically significantly (p<0,05) lower 2,83±0.1 µmol/l compared to the resulting figures of animals comparison group is 3.21±0.12 µmol/L.

In the experimental group of animals activity of ceruloplasmin was statistically significantly (p<0,001) higher 24,23±0,35 USD relative to the comparison group 19,43±0,65 $

For the evaluation of reparative processes in animals of both groups using cytological studies have investigated the dynamic change in the number of neutrophilic leukocytes and fibroblast cells of the row.

The results for the dynamic change of the average number of neutrophilic leukocytes and cells of fibroblastic number of smears-prints on the studied cells from the surfaces of experimental wounds in animals of both groups is given below.

Day researchThe number of neutrons is higher leukocyte
The comparison groupExperienced group
395,45±0,3292,40±0,75
p<0,05
5th90,55±1,0886,15±0,32
p<0,001
7th85,50±0,3258,25±3,34
p<0,001
9th76,05±0,64
p<0,01
34,20±1,4
p<0,001
Day researchThe number of fibroblastic cells of the row
The comparison groupExperienced group
30,9±0,031,1±0,07
5th1,75±0,282,5±0,75
p<0,001
7th6,9±0,6430.20mm±4,75
p<0,001
9th20,5±1,08
p<0,01
63,35±2,05
p<0,001

When this p - level authoritative once the ice performance relative to the comparison group.

Experimental animals were also carried out by histological examination.

By the 10th day in the experimental group had already noted the increase in the thickness of the epidermis by 42.3% compared to the comparison group. In the experimental group the number and structure of blood vessels located in the epidermal layer, had characteristics similar to the normal one, namely the ordered position, typical for organized granulation tissue with the formation of vascular loops. In areas of active circulation was noted by active proliferation of fibroblasts. Dermatovenerology connection was pronounced. The skin was movable, not fused with the underlying tissue. Note also the restoration of the structure of the sebaceous glands, and the presence of hair follicles of varying degrees of maturity and growth of hair. The orientation of the collagen fibers was horizontal.

In rats compared to the 10-th day the skin was tightly fused with the underlying tissue, preserved scab. The number of hair follicles and sebaceous glands per unit area of the slice, in this group there were significantly fewer than in the experimental group. Immature granulation tissue in the wound region was rich in fibroblasts, richly imbued vertically-going vessels. Noted that the arrangement of collagen fibers, mainly chaotic, less vertically along the vessels.

The process of healing in the experimental group were noted, what happened gradual biodegradation wound dressings, which ended almost simultaneously with the end of epithelialization of wounds. Experimental studies have confirmed the effectiveness of using the proposed wound dressings.

Example 2. A method of obtaining a biodegradable wound dressings

In the glass, made of polypropylene, with a volume of 50 ml was poured 15 ml of distilled water. Prepared a sample of food of low molecular weight chitosan (1 g, produced by CJSC "Bioprogress", Moscow in accordance with THE 9289-067-00472124-03. Added a portion of chitosan in a glass of distilled water. The resulting mixture was mixed and placed in a thermostat at 37°C for 2 hours. After 2 hours of chitosan was completely dissolved in distilled water, representing a translucent homogeneous mass of yellow-brown color. Was dissolved 10 mg of lyophilized ceruloplasmin produced by FSUE "Microgen" Russia, Moscow, 1 ml of distilled water, obtaining a clear solution pale blue color. Added to this solution in the mixture. Heated with 10 ml of distilled water in the incubator to 37°C and added 100 mg of L-aspartic acid, produced by JSC "Biosynthesis", Penza. The prepared solution of L-aspartic acid was gradually added to the mixture after each addition by measuring the pH of the solution. Upon reaching pH 5 the procedure of adding a solution of L-aspartic acid in the amount of 1 ml was stopped. Then to the mixture was added 0.5 ml of the plasticizer is glycerol, produced by JSC "Kazan pharmaceutical factory", which amounted to 2.8% of the total volume of the resulting biomass. The latter was mixed with a magnetic stirrer at a speed of 100 rpm for 60 seconds. Using Viscometer SV-10 measured dynamic viscosity of the biomass, which was 26.5 mPas×c. The obtained biomass was poured into a Petri dish, made of polypropylene, covering the entire diameter and forming a uniform layer thickness of 5 mm, and placed for 24 hours in a thermostat at 37°C for drying.

The result was a translucent coating yellow-brown color in the form of a film on one side is glossy, the other rough. The coating is flexible, easily bent and repeated the elevation of the horizontal surface on which it is placed.

Experimental studies confirming the application of the wound dressings described above in example 1.

1. Biodegradative wound covering, made in the form of a film containing chitosan, a plasticizer in the form of glycerin, a solvent, characterized in that it further comprises ceruloplasmin and L-aspartic acid in the following ratio of components, wt.%:
chitosan 5,3 - 5,7
g is iceren 2,2 - 2,8
ceruloplasmin 0,06 - 0,08
L-aspartic acid 0,04 - 0,06
the solvent else
the use of chitosan of low molecular weight dietary chitosan and the solvent with a pH of 5-7.

2. The coating according to claim 1, characterized in that the solvent used saline solution.

3. The coating according to claim 1, characterized in that the solvent used distilled water.

4. The coating according to claim 1, characterized in that the foil has holes with a diameter of 0.2-0.5 mm and the concentration distribution in it 10-15 per 100 mm2.

5. A method of obtaining a biodegradable wound dressings comprising preparing a sample of chitosan, the last breeding in the solvent by adding a sample of chitosan in the solvent mixture and place it in thermostat, add in the mixture of plasticizer in the form of glycerol and placing the obtained biomass in the tank, ensuring the formation of a film by drying the biomass within 18-24 hours, characterized in that the solvent used with pH 5-7, the addition of chitosan prepared from food low molecular weight chitosan and throw it in solvent based 1000 mg of sample in 15 ml of solvent, while the resulting mixture was placed in a thermostat at a temperature 37-42°C for 1-2 hours, then the mixture was added times which can be found in the solvent ceruloplasmin rate of 1:10 with ensuring the formation of homogeneous hydrogel, after dissolving L-aspartic acid in a pre-heated up to 37-40°C the solvent, providing the proportion of 100 mg L-aspartic acid in 10 ml of solvent, and added to the mixture to obtain a pH of the mixture 5-7, the plasticizer is introduced into a mixture of 2.2-2.8% of the total volume of the resulting biomass, the latter is stirred with a speed of 100-150 rpm for 30-60 seconds; the location of the biomass in the vessel is accomplished by providing education uniform coating layer with a height of 3-5 mm, the drying of the coating is performed in a thermostat at a temperature of 37-40°C.

6. The method according to claim 5, characterized in that the film perforined with provision of hole diameter 0.2-0.5 mm and the concentration distribution 10-15 per 100 mm2.



 

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10 cl, 1 tbl, 8 ex

FIELD: medicine.

SUBSTANCE: invention relates to medicine. Described is patch for application in surgery, produced by method, which includes stages of selection of animals tissue, which contains substrate, diametrical linking and fixation of substrate, minimisation of substrate agents activity, substrate tanning and binding of active layer to substrate.

EFFECT: biological patch for surgery does not induce immune rejection and has good biological compatibility.

17 cl, 1 dwg, 1 ex

FIELD: medicine, pharmaceutics.

SUBSTANCE: invention relates to medicine. Described is biological wound covering, produced by method, which includes stages of selection of animal tissue, which contains substrate, of diametrical linking and fixation of substrate, minimisation of substrate agents activity and binding of active layer to substrate.

EFFECT: biological wound covering has good biological compatibility, long-term elasticity, is permeable for gases, but impermeable for bacteria.

20 cl, 1 dwg, 1 ex

FIELD: medicine.

SUBSTANCE: invention relates to medicine. Described is method of manufacturing biologically absorbable bandage, which includes envelope and biologically absorbable microspheres, in form of tourniquet. Additionally bandage envelope contains pores, formed by pore-forming system, which can be activated by external way relative to wound, or form in situ within wound surface. Bandage shape makes it possible to place the bandage on wound surface in such a way that it fills wound by shape and size. Described are bandages in form of tourniquet and their application in combination with treatment by reduced pressure is shown.

EFFECT: bandage in form of tourniquet is easy adapted to size and shape of any wound surface.

25 cl, 11 dwg

FIELD: medicine.

SUBSTANCE: invention refers to medicine and represents an antimicrobial haemostatic sponge made of dispersed collagen after sublimation drying, differing by the fact that it contains collagen, calcium alginate and quinoxidine in a certain ratio, and it is structured in volatile aldehyde steams, and to an antimicrobial haemostatic sponge made of dispersed collagen after sublimation drying, differing by the fact that it contains collagen, formaldehyde and mixed antimicrobial substances in the ratio 10:(0.03-0.10): (0.10-0.30); boric acid and furacyllin in the ratio (4.0-6):3.0 are used as mixed antimicrobial substances specified.

EFFECT: there is offered antimicrobial haemostatic sponge.

2 cl, 3 ex, 1 tbl

Haemostatic sponge // 2385726

FIELD: medicine.

SUBSTANCE: invention relates to medicine, particularly to haemostatic sponges for local haemostasis. The offered haemostatic sponge represents lyophilised 1% collagen in ammonium carbonate with pH 8.5-8.9 which contains feracryl in the following mixing ratio, %: collagen 98.0-99.2, feracryl - 0.8-2.0. The sponge can be additionally textured in vaporised aldehydes and can additionally contain to 3% of antiseptics and 10% of broad-spectrum antibiotics.

EFFECT: invention provides production of the haemostatic sponge exhibiting high haemostatic activity.

3 cl, 5 ex, 1 tbl

FIELD: medicine, pharmaceutics.

SUBSTANCE: invention refers to chemical-pharmaceutical industry and medical equipment and may be used in preparing multifunctional biological active structures for the fixation of dressings and objects. The fixation device consists of a paper carrier made of cellulose and viscous fibres impregnated with a special preparation, dried; with its one side of the prepared carrier coated with an adhesive and with its other side coated with a primer and an adhesive.

EFFECT: improved drape effect, enhancement, easier and faster usage.

6 cl, 1 tbl, 2 ex

FIELD: medicine, pharmaceutics.

SUBSTANCE: invention refers to medicine. What is described is a superabsorbent polymer composite containing superabsorbent polymers and cellulose nanofibrils having a diameter of 100 nm or less. The composite may be presented in the form of either particles, or foam. There are also described methods for preparing the composite and absorbent products containing the superabsorbent polymer composite.

EFFECT: cellulose nanofibrils improve the gel strength of the superabsorbent polymer.

23 cl, 6 dwg, 6 ex

FIELD: medicine.

SUBSTANCE: invention refers to medicine, particularly to surgery, burn and radiation therapy. A dressing contains rayon fabric which at the first stage of carbon cloth production is exposed to ionising radiation of a high-speed electron bunch in cathode-ray current (1-3) mca and power (0.5-0.7) MeV when transported through an electron accelerator exposure chamber at rate (1-4) m/min; the produced carbon cloth is characterised by density 1.3-1.4 g/cm3; surface density 2.5-3.5 m2/g; carbon content 99.6-99.9 wt %; ash content 0.1-0.4 wt %; chlorhexidine absorption 0.6-0.7 g/g if continuously coating the wound surface for 4 days.

EFFECT: dressing is characterised by high sorption dynamics, does not stick to the damaged skin, and does not scar after burns, cleans the wound surface so that reduces a time for wound preparation for closure in 1,5-2 times due to its high mechanical strength and chemical resistance of the carbon cloth after graphitisation at 2400°C; the dressings are re-useable.

3 ex

FIELD: biotechnologies.

SUBSTANCE: medicine is proposed for limitation of calorie consumption in a patient in need of that, including efficient quantity of a polymer hydrogel prepared by the method including stages of aqueous solution production, including polysaccharide and citric acid; and heating of this solution for removal of water and realisation of cross linkage of polysaccharide with citric acid to form the polymer hydrogel. Also the medicine versions are proposed, in which the polysaccharide is carboxymethylcellulose.

EFFECT: limitation of calorie consumption.

37 cl, 2 dwg, 8 tbl, 4 ex

FIELD: medicine.

SUBSTANCE: invention refers to medicine. What is described is a solution for preparing a chitosan material, methods for preparing the haemostatic material of the solution and a medical device with using chitosan fibres. The solution consists of the following ingredients in ratio of total amount of the solution, wt %: dry chitosan with a degree of deacetylation - not less than 80%: 4-8 at dry basis, an aqueous solution of a polymer or a mixture of polymers: 1-10 at dry basis, an aqueous solution of an organic acid or a mixture of organic acids in the concentration of 50 - 80% - the rest. The method for preparing the haemostatic material of the aqueous-acidic solution containing a polyelectrolyte complex of chitosan and a water-soluble polymer comprising the electrochemical treatment of the chitosan solution in an electric field with a conductive substrate. The electrospun fibres have the following characteristics: viscosity - 1.4-2.5 Pa·s, surface tension - 31-35 mN/m and electrical conductivity no more than 2.3 mSm/cm due to the use of a viscoelastic solution.

EFFECT: method enables the chitosan fibre of a thinner diameter.

45 cl, 20 dwg, 6 tbl, 16 ex

FIELD: medicine, pharmaceutics.

SUBSTANCE: invention refers to medicine. What is described is a haemostatic composition containing a biologically absorbable material and hyaluronic acid or a derivative thereof. There are described methods for preparing the given compositions, and there are described the applications of the given compositions. Particularly, the method for preparing the above haemostatic composition involves the dry heat treatment at temperature 110 to 200°C.

EFFECT: compositions exhibit the enhanced haemostatic properties.

68 cl, 1 tbl, 8 ex

Wound tissue // 2483755

FIELD: medicine.

SUBSTANCE: invention refers to medicine, to preparing a therapeutic and preventing agent for treating radioreactions accompanying the course of radiotherapy. A wound tissue contains a textile fibre-porous material with a viscose component coated with a polymer and a drug preparation introduced therein; the novel is the fact that it is suggested to use the textile material which is made of flax-viscose fibres in ratio of components of flax:viscose 40 - 70 and 60-30, respectively. The fibre-porous material is prepared by needle suturing and has area density 180 g/m2 - 260 g/m2. The coating polymer is sodium alginate, while the drug preparation is introduced in the concentration of 0.25-30 wt %. The drug preparation coating the suggested flax-viscose carrier is presented by poorly soluble preparations of Furaginum, metronidazole, 5-fluoruracil, antioxidant Mexidol, biologically active substances - propolis, sea buckthorn and blackberry extracts.

EFFECT: presented drug preparation has proved itself to be easy to use, enabling higher clinical effectiveness, increasing a prolongation time.

6 ex

FIELD: medicine.

SUBSTANCE: present invention refers to medicine, particularly to a method for making hydrogel wound dressings of polyvinylpyrrolidone or its monomer, agar, distilled water, ethylene or propylene, polyglycols, silver using ionising radiation wherein a complex of cationic polysaccharide, preferably chitosan, and silver is prepared by mixing AgNO3 with chitosan powder characterised by an average molecular weight of 40000 to 200000, previously degraded by irradiation with beam energy 10 MeV; the deposition is filtered, washed with distilled water and added to a mixture consisting of, wt %: 2-10% of polyvinylpyrrolidone or monomer, max. 5% of agar, at least 75% of distilled water and 1-3% of ethylene or propylene polyglycols, and gel prepared thereby is cross-linked by ionizing radiation of a dose of 20-30 kGy, preferentially 25 kGy.

EFFECT: provided high biological activity and long effective use of surgical dressings.

3 cl, 6 ex

FIELD: medicine.

SUBSTANCE: composition of forming solution for formation of biopolymeric fibres includes polyethylene oxide, chitosan, organic acid and water. Or composition of forming solution for formation of biopolymeric fibres includes chitosan, water and component, selected from polyethylene oxide, polyvinyl alcohol or polyvinylpyrrolidone. Chitosan with molecular weight 30-40 kDa and polyethylene oxide with molecular weight 2000-8000 kDa are used. Composition additionally contains cellulose diacetate and biologically active substances. Method of forming solution preparation includes mixing of components in powder-like state and their dissolution with mixing to homogenous state. Linen of biomedical purpose, formed from biopolymer chitosan-based fibres, in order to obtain from it bandage for wound treatment, is subjected to swelling in physiological solution or distilled water. Additionally linen of biomedical purpose is subjected to thermal processing, after which it is placed into distilled water or physiological solution for swelling. Or linen is additionally processed with alkaline reagent, with further washing with distilled water, after which it is placed into distilled water for swelling. For wound treatment linen of biomedical purpose is applied on wound in form of biological bandage.

EFFECT: invention makes it possible to obtain biopolymer fibre with application of optimal compositions of textile composition based on chitosan and non-toxic polymer for stable electroformation of defect-free fibres, methods of modification of linen of biomedical purpose for obtaining bandage in form of wound covering are ecologically clean and economical.

2 tbl, 73 ex, 5 dwg

FIELD: medicine.

SUBSTANCE: invention relates to medicine, namely to method of obtaining medical napkin for treatment of wounds and burns. In method of obtaining medical napkin, which includes carrying out textile material padding in solution of medication with the following drying in the air, padding is carried out in solution of sodium alginate polysaccharide with content of 18-22% dimexide, and after drying by method of textile printing water solution of polymer composition containing 18-22% of urea, 5-7% of sodium alginate is applied on textile carrier, with following drying of processed material in the air. Method makes it possible to considerably increase concentration of medication in napkin, considerably extend spectrum of applied medications, including by low-soluble, as it does not require presence of functional groups in medication for immobilisation on functional groups of fibre.

EFFECT: method is simpler in realisation, less durative, does not require additional processing of textile material, ie more manufacturable and economical.

2 ex

FIELD: medicine.

SUBSTANCE: invention refers to medicine. What is described is using active ingredients as exemplified by GML (Glycerol Monolaurate) on a fibrous absorbent structure, such as viscose fibre, used for tampons production, in the very low concentrations enables maintaining effectiveness on the inhibition of synthesis of a toxic shock syndrome toxin 1 (TSST-1) produced by S.aureus, without evident microorganism elimination for the purpose of achieving the desired reduction of the toxin concentration and avoiding undesired impurities.

EFFECT: achieving the desired reduction of the toxin concentration and avoiding undesired impurities.

16 cl, 6 tbl, 3 ex

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