Method of imaging fluorescence traces of object

FIELD: physics, optics.

SUBSTANCE: invention relates to means of analysing fluorescence traces of an object in a displayed image. The method involves generating a video sequence of frames, dividing said sequence into groups in which the inter-frame difference is less than a given threshold value, calculating the average value of digital codes for frame elements within the groups, determining the maximum value from the obtained average values of digital codes for corresponding frame elements, forming an image of fluorescence traces of the object with the maximum value of digital codes.

EFFECT: low level of fluctuation noise in the resultant image due to maximisation of digital codes of elements of average codes obtained beforehand.

2 dwg

 

The invention relates to a television and can be used to create systems television visualization and image analysis traces the illumination of objects in the process of observation. Such objects include, in particular, the glow of the gas discharge, the traces of glowing objects in the sky and other General properties of such images is the presence of a dark background, where there is a bright object, and the number of spatially coincident implementations of the signal from the object is significantly less than the total number of frames contained in the observed sequence. In other words, images of the traces of the glow of such objects have a certain dynamics in the presence of locally sustainable fragments between elements have fairly high degree of correlation.

Known way television visualization of traces glow gas, which consists in memorizing the digital code of one frame of the sequence generated by the television camera. This method is described in the patent of the Russian Federation No. 2141250 MCI A61. The disadvantage of this method is the low image quality due to low signal-to-noise ratio associated with the image sequence.

A method of obtaining images of suppressing fluctuation noise, for localsize in averaging the digital codes corresponding counts brightness all frames of the video sequence, generated by the television camera. This method is described, in particular, in the literature "Digital TV", Ed. by Migratoria. M: Communications. 1980. P.132. The disadvantage of this method is the low image quality due to low signal-to-noise due to the suppression of the desired signal, if the number of spatial matching implementations is small relative to the total number of frames of a video sequence. In other words, the lost features of the structure of the image track, manifested only in some frames of the sequence.

The closest to the technical nature of the claimed method is the visualization of the structure of gas discharge luminosity of an object in an electromagnetic field, which consists in receiving the image signal by a matrix photodetector, converting it into a digital code, the memory and displayed on the monitor screen, while the electromagnetic field is carried out by a single pulse at the end of the quenching pulse frame, and the digital codes of the elements of the frame is compared with a digital code corresponding elements of the frame obtained as a result of the previous comparison, and form the image of the discharge structure elements with the maximum values of the digital codes (Patent No. 2437132 C2, IPC G03B 41/00).

This method allows you with the wound of all traces of illumination of the object, manifested by at least one frame of the sequence. However, the disadvantage of this method is the low degree of suppression of the fluctuation of the noise, because the signal-to-noise image after processing sequence is not high enough.

The main source of noise is the noise output device Converter "light signal" (CCD), which at low contrast the traces of illumination of the object may be partially or completely mask the signal.

The objective of the invention is to improve the quality of the image by increasing the signal-to-noise in the resulting image.

To solve the problem in the visualization of traces of illumination of the object, which consists in receiving the image signal by a matrix photodetector, converting it into a digital code, the memory and displayed on the monitor screen, form a sequence of frames, which are divided into groups in which the inter-frame difference is less than a predetermined threshold value, calculates the average value of the digital codes for the respective frame elements within groups, determine the maximum value of the obtained average values of the digital codes for the corresponding elements of the frame and form the image of the traces of illumination of the object with the maximum digital value is W.

The technical result of the present technical solution is to reduce the level of fluctuating noise and increase the signal-to-noise in the resulting image due to the fact that in each group of frames of the video (i.e. inside almost static scenes) by the formation of the averaged frame, high in 4k times the signal-to-noise ratio, where k is the number of frames in the group. The resulting image is formed by maximizing the digital codes of the elements previously obtained averaged frames.

Technical solutions containing a collection of characteristics that are identical to the features of the invention, it is not revealed that defines the invention according to the criterion of "novelty".

The applicant has not identified any sources of information containing data on the impact of the distinctive characteristics to achieve a result that demonstrates compliance with the invention, the criterion of "inventive step". The method is as follows.

In the television camera 1 of the optical image of the glow through the lens is transferred to the matrix photodetector, an electrical signal which is fed to a standard video recorder - 2 connected to the personal computer 3 for conversion into a digital code, the memory and otobrazheniya screen computer monitor - 4.

The resulting digital codes of the elements of frames of the video sequence is processed in the computer programmatically.

The formation and division of the sequence into groups implement standard algorithms, sorting and searching, sequentially comparing the elements of each individual frame with other frames of the video sequence. If inter-frame difference for the two compared frames below a predetermined threshold, then the decision is made to set the compared frames in the same group. Thus, in each of the groups as a result of processing contains frames with images of the traces of luminous objects, between the elements of which there is a high degree of correlation. In turn, the degree of correlation is determined by a set threshold values for interframe difference.

Averaging corresponding samples of brightness within groups is performed in accordance with the following expression:

Ui=1kj=1kUjwhere k is the number of frames in the group, Ujdigital codes of the elements (counts brightness) of the current frame.

When maximizing the processing of the averaged frame is inside the groups of the video sequence in accordance with the following expression:

Umaxi={Ui,ewith alandUiUi-1Ui-1,ewith alandUi<Ui-1(1),

where i=1, 2...n, Uidigital codes of the elements (counts brightness) of the current frame, Ui-1digital codes of the elements (counts brightness) of the frame obtained by the previous comparison, n is the number of frames of a video sequence.

Get the resulting image memorize and display on a computer monitor.

As a matrix of a sensor can be used for virtually any CCD camera standard sensitivity, as the video capture device can be used for virtually any standard device, such as a type Aver EZ Capture firm Aver Media connected to the PCI bus of the computer.

The resulting image may be obtained, for example, by programming in the environment of the standard MATLAB package.

So the m way to implement this method used known materials and equipment, which leads to the invention according to the criterion of "industrial applicability".

Practically this method can be applied for visualization of traces of gas discharge glow, and other light objects on a dark background, for example for visualization of the luminous traces of moving objects in the night sky.

As an example, figure 2 shows the results of processing a video sequence containing a luminous object (planet Venus) in the night sky, the proposed method with the aim of forming a trace (trajectory) in comparison with the method of maximization and the method of averaging. As can be seen from the images and waveforms of a signal along a selected line, when processed by the proposed method achieves higher signal-to-noise compared with treatment by maximizing or averaging the entire sequence. Indeed, compared with the maximization of the entire sequence, the proposed method achieves a lower level of fluctuation noise at the same level of the useful signal. In comparison with the method of averaging the proposed method provides a higher level of the useful signal at slightly different largest level fluctuation noise./p>

The visualization of traces of illumination of the object, which consists in receiving the image signal by a matrix photodetector, converting it into a digital code, the memory and displayed on the monitor screen, wherein forming the sequence of frames, which are divided into groups in which the inter-frame difference is less than a predetermined threshold value, calculates the average value of the digital codes for the respective frame elements within groups, determine the maximum value of the obtained average values of the digital codes for the corresponding elements of the frame and form the image of the traces of illumination of the object with the maximum value of the digital codes.



 

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