Experimental method for elimination of pathogenic and opportunistic microorganisms

FIELD: medicine.

SUBSTANCE: invention refers to disinfection. The experimental method for the elimination of pathogenic and opportunistic microorganisms is characterized by the fact that a mixture containing the pathogenic and opportunistic microorganisms are processed with a disinfection composition which is metal oxyhydrate gel prepared by alkaline metal chloride deposition by 2% ammonia in a min. 5-litre reactor; fresh metal oxyhydrate 20-10-6 m3 is placed in an electrochemical cells with graphite electrodes of a charged cluster metal particle generator; what is added is a bacterial solution of the medium of 105 microbial cells in 1 ml dissolved in distilled water 10 ml; the medium is exposed to polarized currents of the cluster oxyhydrate particles of metal oxyhydrate gel for 2 to 6 hours; metal oxyhydrate gels are prepared by using metal chloride salts presented by zirconium or iron chloride salts with the electrode distance no more than 70 mm.

EFFECT: invention provides the more effective inactivation of pathogenic and opportunistic microorganisms that are infectious agents in human and animals.

7 tbl, 2 dwg, 6 ex

 

The invention relates to the field of disinfection and can be widely used in health food and pharmaceutical industries, public utilities, for the disinfection and preservation of drinking water for disinfection of swimming pools.

Known RF patent №2414912, in which the first proposed method for disinfecting an aqueous solution containing silver ions, obtained by electrolysis of distilled water and food acid, characterized in that it contains as a food acid, lactic acid, and 33%hydrogen peroxide in the following ratio of components, g/l: silver ions Ag+0,01-1,5; lactic acid - 1-50; hydrogen peroxide 33% - 0,1-3; distilled water-the rest, the electrolysis is performed with the use of two silver electrodes during periodic change their polarity.

With the second method proposed disinfecting aqueous solution containing silver ions, obtained by electrolysis of distilled water and food acid, characterized in that it contains as a food acid, lactic acid, copper ions, obtained by electrolysis, and 33%hydrogen peroxide in the following ratio of components, g/l: silver ions Ag+-0,01-1,5; copper ions Cu+-0,04-4; lactic acid - 1-50; peroxide water is sort of 33%-0,1-3; distilled water - the rest, the electrolysis is performed with the use of silver and copper electrodes during periodic change their polarity.

The disadvantage of this method is that during long-term storage bactericidal activity of this solution is reduced and loses its transparency. This is because silver ions interact with substances in the water, resulting in the concentration of silver ions decreases, and the interaction products precipitate.

Known RF patent №2176505, in which method the destruction of pathogenic and conditionally pathogenic microorganisms using water-soluble antiviral and antibacterial drugs, broad-spectrum based on the complex preparations ion and silver ion gold casinowin dye (methylene blue) in the following molar ratio: N,N'-tetramethylene:Ag:Au=2:1:1, corresponding to the composition: C32H36N9About9S2ClAgAu. The synthesis of the drug was carried out as follows: when the molar ratio of the reagents N,N'-tetramethylpyrazine nitrate:Ag:Au:C2H5HE=2:1:1:1 in water was dissolved methylene blue (N,N'-tetramethylpyrazine chloride), which were converted into the nitrate form by adding a solution of silver nitrate (AgNO3). Then the precipitated AgCl was filtered, and the floor is built to nitrate solution of methylene blue was again added to the silver nitrate solution, then the drug ion gold (ul3or HAuCl4·3H2O) and ethyl alcohol, heating is conducted to a temperature of 80-95°C, with the subsequent direction of the reaction mass, containing 10-20 wt.% solid reagents on a spray dryer or a vacuum rotary evaporator.

The disadvantage of this method is that the receipt of this medicinal substance presents certain technical difficulties.

The closest analogue, selected as a prototype, is a method of destroying pathogenic and conditionally pathogenic microorganisms, described in the patent of Russia №2430756, published on 10.10.2011, characterized by the fact that simultaneously process the area containing the microorganisms, the composition of a photosensitizer or photocatalyst, maintain within the time period required for efficient binding of the composition to the cells of the microorganisms;

then act on the specified area of the optical radiation with wavelengths corresponding to the maximum absorption of the photosensitizer or photocatalyst, and power density for the activation of the composition, while in use as a photosensitizer methylene blue or brilliant green, and as the photocatalyst used nanoparticles of titanium dioxide, while affected by shirokopolosnaya, laser or led optical radiation, the wavelengths of the radiation is chosen in the range from 390 to 440 nm and from 600 to 670 nm, the radiation power density is chosen in the range from 15 to 50 mW/cm2the duration of exposure is from 1 to 30 min, the concentration of methylene blue is 0,0025 to 0.0001%, the concentration of brilliant green is 0,000125 to 0,00001%, the concentration of nanoparticles of titanium dioxide is from 0.1 to 0.01%, the time period required for efficient binding of the composition to the cells of the microorganisms, is from 10 to 20 minutes

The disadvantage of this method is its lack of effectiveness, due to the fact that the implementation of this method presents certain technical difficulties associated with the use of laser or led optical radiation, and the use of methylene blue adversely affect the normal microbial composition of the medium influenced.

The present invention is to develop a method for efficient and selective inactivation of pathogenic and conditionally pathogenic microorganisms cause infectious disease in humans and animals.

The technical result consists in selectivity inactivation of pathogenic and conditionally pathogenic microorganisms (in the absence of the negative impact nenormalnoi flora and the body's own cells) and in increasing the efficiency of inactivation of pathogenic and conditionally pathogenic microorganisms.

The technical result is achieved in that in the method of destroying pathogenic and conditionally pathogenic microorganisms, characterized in that the medium containing pathogenic and conditionally pathogenic microorganisms, process disinfecting composition according to the invention, the disinfectant composition is a gel oxyhydrate metal, which is obtained by alkaline precipitation from a salt solution of the metal chlorides 2%solution of ammonia in a reactor volume of at least 5 l of freshly prepared gel oxyhydrate metal volume 20·10-6m3placed in the electrochemical cell with graphite electrodes for the formation of charged cluster particles of metal and add bacterial solution environment 105microbial cells in 1 ml diluted with 10 ml of distilled water and affect the environment of the polarization streams cluster oxyhydrate gel particles oxyhydrate metal from 2 to 6 hours, while for the formation of gels oxyhydrates metals as salts of chlorides of metals selected salts of chlorides of zirconium or iron, the distance between the electrodes is not more than 70 mm

Due to the fact that the method of destruction of pathogenic and conditionally pathogenic microorganisms disinfectant composition is a gel oxyhydrate metal, which is obtained by alkaline precipitation from solution with the Lee metal chlorides 2%solution of ammonia in a reactor volume of at least 5 l, the fresh gel oxyhydrate metal volume 20·10-6m3placed in the electrochemical cell with graphite electrodes for the formation of charged cluster particles of metal and add bacterial solution environment 105microbial cells in 1 ml diluted with 10 ml of distilled water and affect the environment of the polarization streams cluster oxyhydrate gel particles oxyhydrate metal from 2 to 6 hours, while for the formation of gels oxyhydrates metals as salts of chlorides of metals selected salts of chlorides of zirconium or iron, the distance between the electrodes is not more than 70 mm, increases the selectivity of inactivation of pathogenic and conditionally pathogenic microorganisms (absence of negative impact on normal flora and the body's own cells) and increases the efficiency of inactivation of pathogenic and conditionally pathogenic microorganisms.

This technical result is ensured by the joint action of the aggregate used in the method of destroying pathogenic and conditionally pathogenic microorganisms gels oxyhydrates metals and polarization pulsed currents of self-organization of gel oxyhydrate metal from 2 to 6 hours

The claimed invention is not known from the prior art, this solution has novelty.

Given the above, can make the substance of the conclusion, the proposed method of destruction of pathogenic and conditionally pathogenic microorganisms is not obvious from the prior art and the whole set of essential features showing a new feature that enables you to achieve the specified technical result, that is, the invention meets the eligibility criterion of "inventive step".

The claimed invention meets the criterion of "industrial applicability", as it will find wide application in health care, food and pharmaceutical industries, public utilities, for the disinfection and preservation of drinking water for disinfection of swimming pools.

The invention consists in the following.

The invention is illustrated by drawings:

figure 1 - diagram of the process of cultivation of microbial culture in sterile distilled water and the gel;

figure 2 - installation of the experimental colloid-chemical cell with graphite electrodes for the study of spike bursts nanoclusters (current self) oxyhydrate gels in the bacterial environment.

In the way of the destruction of pathogenic and conditionally pathogenic microorganisms suggested usage oxyhydrate gels of zirconium, iron for the disinfection of aqueous media from bacterial components, namely bactericidal action of gels OKS the hydrate of zirconium, iron on Escherichia and Pseudomonas aeruginosa, as well as to the causative agent of dysentery.

The instrument for the detection and measurement cluster particles - experimental colloid-chemical cell with graphite electrodes for the study of spike bursts nanoclusters (current self) oxyhydrate gels in the bacterial environment is a rectangular electrochemical cell, the ends of which are fixed graphite electrodes. The distance between the electrodes 70 mm or less (figure 2). The contact electrodes are connected to an electronic evaluation unit (if necessary). Installation is described in the patent of Russia №2300161 published 27.05.2007,

The fresh gel with bacterial medium was placed in such an electrochemical cell. Preparation of a gel oxyhydrate metal by alkaline precipitation from a salt solution of the metal chlorides 2%solution of ammonia in the reactor described in the same patent.

In the fresh gel oxyhydrate metal volume 20·10-6m3added bacterial solution environment 105microbial cells in 1 ml diluted with 10 ml of distilled water, and influenced the environment of the polarization pulse currents of self-organization of gel oxyhydrate metal from 2 to 6 hours. The cell was short-circuited, the value of the output resisting film to prevent the effect was insignificant. Nanotek occurring in the system, measured by a special electronic equipment with a sampling frequency of 5 times per second. The experiment lasted for 5-6 hours.

For bacteriological studies used such culture conditionally pathogenic bacteria like Escherichia coli (E. coli), Shigella Flexneri (the Shigella Flexner - the causative agent of dysentery) and Pseudomonas aeruginosa (Pseudomonas aeruginosa).

The determination of the number of sown bacterial cells was carried out on the optical standard turbidity. The essence of the method consists in comparing the turbidity of the used bacterial suspension with a turbidity control optical standard. Compare mist was in the tube with the same inner diameter, thickness and color of the glass. In each of the original culture was kept conditionally 10 microbial cells in 1 ml of suspension (M.L./ml).

From this suspension was performed serial 10-fold dilution in sterile distilled water (DW).

This took 3 sterile tubes, marked them DW No. 1 DW No. 2, LW No. 3, and in each of them poured in 9 ml of sterile distilled water. In a test tube LW No. 1 was made with 1.0 ml of the test tubes with a large suspension of microbial cells by optical standards, suspension is thoroughly mixed. For further breeding of the tubes LW No. 1 was transferred to 1.0 ml of the active ingredient No. 2, thoroughly mixed, is here 1.0 ml was transferred to the next tube.

Scheme cultivation culture standard from 105microbial cells are presented in table 1.

Table 1
Rooms tubesThe number of sterile distilled water, mlThe amount of the introduced suspension culture of the original mlBreedingThe approximate number of microbes in 1 ml of suspension, M.L./ml
LW No. 19,01,0 from the fraction containing the 105M.L./ml10-110000
LW No. 29,01,0 from LW No. 110-21000
LW No. 39,01,0 from LW No. 210-3100

Then spent the sowing of 0.02 ml of bacterial suspension in sterile distilled water test tubes DW No. 1 DW No. 3 after 2, 4 and 6 hours after finding bacterial cultures in distilled water and received the control experiments.

Then cooked again is edenia in oxyhydrate gels. This was placed in 9 ml of gel 3 tubes (G No. 1 - D No. 3) and did the following: 1 ml of bacterial suspension from the tube with the concentration of bacteria 105was added in the tube with the gel (G # 1) and received respectively dilution 10-1.

Then 1 ml of bacterial suspension from the tube LW No. 1 dilution 10-1added in the tube with the gel (G 2) and received respectively dilution 10-2. The same was done with the tube LW No. 2 and received the dilution 10-3in the gel (G №3). The process of cultivation is presented in figure 2.

Then did the sowing of 0.02 ml of bacterial suspension from each tube with gel after 2,4 and 6 hours after finding bacterial cultures in the gel.

Then prepared the bacterial environment in oxyhydrate gels, exploring the influence of the electrochemical cell. This was placed in 9 ml of gel in 3 of the cell unit (P # 1 - P # 3) and did the following: 1 ml of bacterial suspension from the tube with the concentration of bacteria 105added in the cuvette cell gel (P # 1) and received respectively dilution 10-1.

Then 1 ml of bacterial suspension from the tube LW No. 1 dilution 10-1added in a cuvette with gel (P # 2) and received respectively dilution 10-2. The same was done with the tube LW No. 2 and received the dilution 10-3in the device cell with gel (Item No. 3).

Then spent the sowing of 0.02 ml of bacterial usues the th of each cell of the device with gel after 2,4 and 6 hours after finding bacterial cultures in a cell of the device with gel.

Sowing was made on a nutrient medium Endo in Petri dishes. Then Cup thermostatically for days at 37°C, and made calculations grown bacterial colonies (CFU).

When analyzing the actions of gels oxyhydrates on bacteria, the action of the device with gel oxyhydrate bacteria compared the number of grown colonies from samples LW No. 1 - D No. 1 - P # 1, LW No. 2 - D No. 2 - P No. 2, LW No. 3 - G 3 - P No. 3. Electrochemical cell - the unit used in the studies presented in figure 2

The results of the study of antimicrobial activity of gel oxyhydrate Zirconia.

To study the antimicrobial action of oxyhydrate gel Zirconia used cultures of such bacteria as Escherichia coli (E. coli), Shigella Flexneri (the Shigella Flexner - the causative agent of dysentery and Pseudomonas aeruginosa (Pseudomonas aeruginosa) with the aforementioned method.

Example 1 Experimental results of studies number of grown colonies of the bacteria Pseudomonas aeruginosa (Pseudomonas aeruginosa) are presented in table 2

Table 2
Initial concentration (the number of bacteria in 1 ml solution)The growth of microbial cells in distilled water, M.L./ml The growth of microbial cells in the gel without electrochemical cell, M.L./mlThe growth of microbial cells in the electrochemical cell, M.L./ml
2 hoursAfter 4 hoursAfter 6 hours2 hoursAfter 4 hoursAfter 6 hours2 hoursAfter 4 hoursAfter 6 hours
104CPF.* growthCPF. growthCPF. growthCPF. growthCPF. growthCPF. growthCPF. growthCPF. growthCPF. growth
103CPF. growthCPF. growthCPF. growthCPF. growthCPF. growthCPF. growthCPF. growthCPF. growthCPF. growth
102 CPF. growthCPF. growthCPF. growthCPF. growthCPF. growthCPF. growth688540282
* Solid growth

It is known that Pseudomonas aeruginosa (Pseudomonas aeruginosa) has low sensitivity to most antibiotics. As can be seen from table 2, either distilled water or investigational oxyhydrate gel, Zirconia does not have antimicrobial activity against this bacteria cultures. The number of colonies over time spent in the gel decreases only for the smallest initial concentration of bacteria 102under the influence of pulsed polarization self-organization current in the electrochemical cell.

Example 2 Experimental results of the number of grown colonies of coliform bacteria (Escherichia coli).

Table 3 summarizes the results of the influence of the gel (including in the electrochemical cell) on bacteria Escherichia coli (E. coli).

Table 3
Initial concentration (the number of bacteria in 1 ml of the solution is) The growth of microbial cells in distilled water, M.L./mlThe growth of microbial cells in the gel without electrochemical cell, M.L./mlThe growth of microbial cells in the gel in the electrochemical cell, M.L./ml
2 hoursAfter 4 hoursAfter 6 hours2 hoursAfter 4 hoursAfter 6 hours2 hoursAfter 4 hoursAfter 6 hours
104CPF. growthCPF. growthCPF. growthCPF. growthCPF. growthCPF. growthCPF. growth714118
103CPF. growthCPF. growthCPF. growthCPF. growthCPF. growthCPF. growth928 10526
102296302314100000

The analysis of table 3 showed that E. coli (Escherichia coli) is very sensitive to the incumbent on her to oxyhydrate gel Zirconia separately and in combination with the polarizing pulse currents.

Antimicrobial effectiveness of the gel in the electrochemical cell is observed for all initial concentrations of bacteria. Antimicrobial activity of pure gel was observed only at very small concentrations of bacteria. In the gel of zirconium oxyhydrate under the influence of pulsed partial nanoclusters zirconium oxyhydrate concentration of bacteria decreased in 10 times or more, and in the last breeding colony growth of bacteria is not observed.

Example No. 3 Experimental results of the number of grown colonies of bacteria of the Shigella Flexner - the causative agent of dysentery (Shigella Flexneri) are presented in table 4.

Table 4
Original to ncentrate (the number of bacteria in 1 ml of the original solution) The growth of microbial cells in distilled water, M.L./mlThe growth of microbial cells in the gel without electrochemical cell, M.L./mlThe growth of microbial cells in the gel in the electrochemical cell, M.L./ml
2 hoursAfter 4 hoursAfter 6 hours2 hoursAfter 4 hoursAfter 6 hours2 hoursAfter 4 hoursAfter 6 hours
104CPF. growthCPF. growthCPF. growthCPF. growthCPF. growthCPF. growthCPF. growthCPF. growthCPF. growth
103CPF. growthCPF. growthCPF. growthCPF. growthCPF. growthCPF. growthCPF. R is St 92896
102CPF. growthCPF. growthCPF. growthCPF. growth230064014040

In oxyhydrate gel, zirconium in the electrochemical cell the growth of bacteria of the Shigella Flexner significantly slows down and almost stops completely after a long (six-hour) exposure to relatively low concentrations of bacteria. However, the antimicrobial effect of oxyhydrate gel of zirconium on the culture of the Shigella Flexner - the causative agent of dysentery less compared to the effect on Escherichia coli, as without electrochemical cell and electrochemical cell.

Conclusions: the effect of oxyhydrate gel Zirconia on Pseudomonas aeruginosa is rather weak; antimicrobial activity gel of E. coli is very effective, leads to a complete halt its growth. For the Shigella Flexner-the causative agent of dysentery action gel better in comparison with Escherichia coli, but the complete cessation of growth of the bacterial culture solution has managed to achieve only after 6 time-keeping is of astora in the electrochemical cell.

The results of the study of antimicrobial activity of gel oxyhydrate iron.

To study the antimicrobial action of iron oxyhydrate gel used cultures of such bacteria as Escherichia coli (E. coli), Shigella Flexneri (the Shigella Flexner) and Pseudomonas aeruginosa (Pseudomonas aeruginosa) with the aforementioned method.

Example No. 4. Experimental results of the number of grown colonies of the bacteria Pseudomonas aeruginosa (Pseudomonas aeruginosa) are presented in table 5.

Table 5
Initial concentration (the number of bacteria in 1 ml solution)The growth of microbial cells in distilled water, M.L./mlThe growth of microbial cells in the gel without electrochemical cell, M.L./mlThe growth of microbial cells in the gel in the electrochemical cell, M.L./ml
2 hoursAfter 4 hoursAfter 6 hours2 hoursAfter 4 hoursAfter 6 hours2 hoursAfter 4 hoursAfter 6 h the owls
104CPF. growthCPF. growthCPF. growthCPF. growthCPF. growthCPF. growthCPF. growthCPF. growthCPF. growth
103CPF. growthCPF. growthCPF. growthCPF. growthCPF. growthCPF. growth19181192574
102CPF. growthCPF. growthCPF. growth86463249245013318

From the table it follows that the growth of colonies of Pseudomonas aeruginosa decreased under the influence of gel throughout the time of the study. Greater impact on this process is provided an electrochemical cell. After 6 hours exposure and growth of microbial cells is meiselsa to 18 M.L./ml However, the electrochemical cell with iron oxyhydrate gel did not lead to the complete destruction of Escherichia coli. Probably with longer exposure and a lower concentration of bacteria is possible to achieve complete destruction of bacterial culture of Pseudomonas aeruginosa in solution.

Example No. 5. Table 6 provides the results of the effects of gel oxyhydrate iron (including in the electrochemical cell) on bacteria Escherichia coli (E. coli), experimental results of the number of grown colonies of coliform bacteria (Escherichia coli).

During the whole time of the experiment, the growth of colonies of culture of Escherichia coli (E. coli) was decreased (especially in the electrochemical cell). It can be assumed that the gel oxyhydrate of iron in the cell inhibits the development and growth of bacteria in this culture, but not as effective as the gel of zirconium oxyhydrate.

Example No. 6. Table 7 contains the results of a research study of antimicrobial activity of gel oxyhydrate of iron in relation to culture bacteria of the Shigella Flexner, experimental results of the number of grown colonies of bacteria of the Shigella Flexner (Shigella Flexneri).

Table 7
Initial concentration (the number of bacteria in 1 ml solution)The growth of microbial cells in distilled water, M.L./MT.The growth of microbial cells in the gel without electrochemical cell, M.L./mlThe growth of microbial cells in the gel in the electrochemical cell, M.L./ml
2 hoursAfter 4 hoursAfter 6 hours2 hoursAfter 4 hoursAfter 6 hours2 hoursAfter 4 hoursAfter 6 hours
104CPF. growthCPF. growthCPF. growthCPF. growthCPF. growthCPF. growthCPF. growthCPF. growthCPF. growth
103CPF. growthCPF. growthCPF. growthCPF. growthCPF. growth CPF. growthCPF. growthCPF. growth520
102CPF. growthCPF. growthCPF. growthCPF. growthCPF. growthCPF. growth834580198

According to the development of bacterial colonies the Shigella Flexner you can say that in this case, the concentration of the bacterial culture is greatly reduced in the cell with iron oxyhydrate gel with a long six-hour exposure of the gel oxyhydrate iron.

According to the results of impact studies oxyhydrate gel iron on Escherichia coli and Shigella the Flexner we can conclude that only after prolonged exposure of the gel oxyhydrate iron, there is a slight decrease in the growth of bacterial cultures. Action gel oxyhydrate of iron to Pseudomonas aeruginosa much more effective action of oxyhydrate gel Zirconia.

Thus, the antimicrobial activity is increased in the range of oxyhydrate iron - oxyhydrate zirconium. The highest sensitivity with respect to the zirconium oxyhydrate gels and iron showed the T. E. coli.

The mechanism of action of gels oxyhydrates next.

Zirconium compounds and other heavy metals have known antimicrobial effect by themselves. Ions of zirconium, for example, can be included in the life cycle of bacteria, disrupting their activity.

When forming oxyhydrate matrix in unstructured colloidal environment leads to the formation of helical particles. Spiral particles consist of parallel polymer chains (or layers)that form in the space spiral of education, which have metadatamodel ordering. In the gel are structureless units, representing a low molecular weight polymer particles without the expressed order.

Spiral fragments oxyhydrates metals is formed on the surface of the system of the electric double layers. As a result of drift of colloidal particles oxyhydrates and ions in the medium between the electrodes there is a potential difference. The flow of ionic clusters is accompanied by destruction of adsorption (on the stern) and the diffusion layers ECT (electric double layer) oxyhydrate fragments, which will lead to the polarization of the electrical double layer. This polarization ECT recurrent dissociative and conformational rearrangements occurring in the macromolecular oxyhydrates, metals, may decay with the emission of ion clusters.

As mentioned earlier, Jonah cast system due to conformational rearrangements occurring in the metal oxyhydrates, oscillate with a certain frequency and intensity (in conditions far from equilibrium). They are able to create turbulence in the reaction system. Perhaps these eddies are distributed in the cytoplasm of bacteria (bacterial reaction medium), complicating its vital function, in addition, those charged oxyhydrate clusters change while the charge of pathogenic bacteria that leads to their destruction.

Thus, the antimicrobial activity of nanoclusters oxyhydrates increases in the range of oxyhydrate iron - oxyhydrate zirconium. Less impact of zirconium oxyhydrate gels and iron shows Pseudomonas aeruginosa, and the most high - Escherichia coli (Sukharev SCI, apalikov YOU, Lebedev YU, Kuznetsov A. C. Impact of nanoclusters oxyhydrate colloids on bacterial culture. Butlerov communications. 2011. V.27. No. 15. - P.34-48.).

In the present method of destroying pathogenic and conditionally pathogenic microorganisms increases the selectivity of inactivation of pathogenic and conditionally pathogenic microorganisms (absence of negative impact on normal flora and the body's own cells) and increases the effect of what you want to make inactivation of pathogenic and conditionally pathogenic microorganisms, due to the fact that the disinfectant composition is a gel oxyhydrate metal, which is obtained by alkaline precipitation from a salt solution of the metal chlorides 2%solution of ammonia in a reactor volume of at least 5 l of freshly prepared gel oxyhydrate metal volume 20·10-6m3placed in the electrochemical cell with graphite electrodes for the formation of charged cluster particles of metal and add bacterial solution environment 105microbial cells in 1 ml diluted with 10 ml of distilled water and affect the environment of the polarization streams cluster oxyhydrate gel particles oxyhydrate metal from 2 to 6 hours, while for the formation of gels oxyhydrates metals as salts of chlorides of metals selected salts of chlorides of zirconium or iron, the distance between the electrodes is not more than 70 mm

Experimental method the destruction of pathogenic and conditionally pathogenic microorganisms, characterized in that the medium containing pathogenic and conditionally pathogenic microorganisms, process disinfecting composition, wherein the disinfectant composition is a gel oxyhydrate metal, which is obtained by alkaline precipitation from a salt solution of the metal chlorides 2%solution of ammonia in a reactor volume of at least 5 l of freshly prepared gel is cigerate metal volume 20·10 -6m3placed in the electrochemical cell with graphite electrodes for the formation of charged cluster particles of metal and add bacterial solution environment 105microbial cells in 1 ml diluted with 10 ml of distilled water and affect the environment of the polarization streams cluster oxyhydrate gel particles oxyhydrate metal from 2 to 6 h, while for the formation of gels oxyhydrates metals as salts of chlorides of metals selected salts of chlorides of zirconium or iron, the distance between the electrodes is not more than 70 mm



 

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FIELD: medicine, pharmaceutics.

SUBSTANCE: invention relates to bactericidal medication and can be applied for disinfection of livestock premises, placed in them technological equipment, implements for animal care, vehicles for transportation of animals, places where animals and other objects of veterinary supervision are concentrated. Medication includes alkyldimethylbenzylammonium chloride (ADMBAC), glutaric aldehyde, water and sodium hydroxide, taken in specified ratio. In case of specified ratio synergetic effect is observed and maximal bactericidal activity of medication, assessed by value of minimal bactericidal concentration (MBC), at which death of test-culture is observed, is reached.

EFFECT: invention ensures increase of bactericidal activity and reduction of medication toxicity with minimal content of active substances.

1 tbl, 15 ex

FIELD: medicine, pharmaceutics.

SUBSTANCE: invention relates to sanitation and can be applied in food, medical, veterinary, processing industry. Invention can be applied for obtaining preparations for disinfection of surfaces in premises, sanitary-technical equipment, items patient care, claimed disinfection preparation can also be applied for disinfection and pre-sterilisation cleaning of products of medical purpose, for disinfection and processing hands of medical personnel. Disinfection preparation includes quaternary ammonium compound, boric acid and represents water solution of quaternary ammonium compound, as such it contains diethyldichloropropenylammonium chloride or diethyldiallylammonium chloride, with the following ratio of initial components, wt %: said quaternary ammonium compound - 1.2 wt %, boric acid - 2.3 wt %, water - the remaining part.

EFFECT: invention ensures increase of bactericidal and fungicidal activity with reduction of concentration of working solutions of preparation, extension of action spectrum, including action on mycobacteria.

4 tbl, 1 ex

FIELD: medicine, pharmaceutics.

SUBSTANCE: invention refers to medicine. What is described is a composite material which contains a non-organic substance in the form of a layer or a layer ingredient with the non-organic substance leads to form hydrogen cations in contact with an aqueous medium that induces an antimicrobial effect with the substance being produced of MoO2, MoO3, molybdenum carbide, molybdenum nitride, molybdenum silicide, molybdenum sulphide, molybdenum hexacarbonyl and/or molybdenum acetylacetonate.

EFFECT: antimicrobial effect of the composite material is found through time almost with no limit.

32 cl, 3 tbl

FIELD: process engineering.

SUBSTANCE: invention relates to device applying electric field to pumped medium. Proposed device comprises two power supplies to generate electric voltage including voltage converter, electric charge accumulator and switchboard, nonconducting chamber for medium pumping including first and second electrode plates, and control unit to control said power supplies. Said control unit performs synchronized control over switchboards of every power supply by 0.1-100 ms-long pulse signal to establish serial connection between power supplies, first and second electrode plates. Resultant total voltage builds up between first and second electrode plates while said resultant total voltage generates electric field there between to subject pumped medium to electric field effects. Chamber medium pumping chamber is composed by piston pump, piston outer surface making one of said electrode plates. Note here that said pump is communicated with feed inlet and outlet tubes.

EFFECT: efficient processing.

27 cl, 4 dwg

FIELD: medicine.

SUBSTANCE: group of inventions refers to microorganism elimination from fluid or gas dielectric mediums. A method of microorganism elimination from dielectric mediums consists in the fact that said medium is passed through a current-conducting flat electrode system whereto DC high voltage ranging 5 to 25 kV is supplied pairwise. The electrodes are arranged with their surfaces facing each other at 2 to 6 mm, making thereby pairs of supplied negative and positive electrodes, and the first electrode from a side of medium feed is negative. A device for implementing said method comprises a cylindrical body at the ends of which there are covers with sleeve unions for dielectric medium supply and drainage and which integrates a electrode assembly detachable both from each other, and from the body by dielectric pads. The electrodes are connected pairwise to different poles of a DC high-voltage source ranging within 5 to 25 kV; and the negative electrode represents a slotted plate provided with pointed petals or needles, while the positive electrode represents a non-corrosive metal grid.

EFFECT: group of inventions provides microorganism elimination from fluid or gas dielectric mediums.

2 cl, 2 dwg

FIELD: medicine.

SUBSTANCE: invention refers to feed decontamination. The farm animal feed is exposed to electromagnetic field. Said electromagnetic field represents a low-frequency electromagnetic field of power 0.1-10 Wt for 30-90 min, frequency 4-500 Hz at temperature 4-40°C.

EFFECT: method provides more effective farm animal feed decontamination from infectious agents.

6 ex

FIELD: medical equipment.

SUBSTANCE: device for purification and sterilisation of passages of tubular elongated products contains central electrode mounted in longitudinally movable insulating bush, carrying second electrode, said electrodes being connected with source of electric impulse discharges are being placed into treated passage filled with pumped through working liquid. Central electrode is made with trough axial opening and is equipped with resting upon butt of isolating bush flange, mounted from rod electrode side. Rod electrode is fixed coaxially with central electrode by means of blackets of cylindrical current-supply, enveloping insulating bush and adjoining treated passage surface with guaranteed ring clearance. Discharge surface of central electrode flange is made of concave form.

EFFECT: compact and mobile device for automatic complex treatment which includes purification and sterilisation of passages of elongated tubular products.

2 cl, 1 dwg

FIELD: medicine.

SUBSTANCE: invention can be used in prevention of purulent process in the area of the post-operational osseous defects. 3% poviargol is added to the bioactive glass-ceramic spongeous byositall implant, at 18° ÷ 3°C by use of electrophoresis within 30 min at current intensity 50 mA; the saturation of implants with antiseptic is performed under the load until the hydrophilic gasket is firmly adhered to the implants.

EFFECT: long-lasting antibacterial effect is provided inside infected osseous wound.

3 dwg, 3 tbl

FIELD: medicine.

SUBSTANCE: method involves carrying out electrolyte electrolysis. The electrolyte contains alkaline metal salt solution in distilled water. Electrolysis is carried out with electrodes having 99.99 silver content 5-12 mm far from each other in electrolyte as solution of sodium acetate buffer of pH=3.6-5.6, current intensity of 0.15-5.0 mA/cm2 and voltage of 3-12 V. Free silver ions quantity grows in the solution to theoretically significant values.

EFFECT: high biological activity; improved bactericide and regenerative properties; prolonged solution storage time.

2 cl, 1 tbl

FIELD: animal science, poultry science, fur farming, combined feedstuff industry.

SUBSTANCE: the present innovation deals with disinfecting fodders contaminated with, for example, the virus of plague, influenza, foot and mouth disease or the agents of other infections and , also, with detoxicating the fodders infected with fungal toxins (aflotoxin, zearalidone, T-2 and others). The present innovation deals with treating grain and coarse fodders with disinfecting solution as neutral anolyte at pH=7-8, concentration of oxidants ranged 0.02-0.06% and redox potential being +100050 mV obtained due to affecting 0.02-0.04%-sodium chloride with direct current, moreover, the treatment with disinfecting solution should be fulfilled at about 15-25°C for 60-120 min at 1.5-2.0l/kg fodder. The innovation enables to increase the efficiency of disinfecting fodders infected with pathogenic bacteria, viruses, fungi and their toxins.

EFFECT: higher efficiency of disinfection.

6 ex

FIELD: medical instruments.

SUBSTANCE: apparatus can be used for automated disinfections, pre-sterilization cleaning and for sterilization of medical equipment and facilities of laboratories and departments of clinics. Apparatus has electrochemical activator connected in series with ejector, rotameter, flow indicator, throttle, liquid pressure controller, flow detector, gas separator, controlled throttle, electronic control unit with built-in processor and anolyte level detector and automaton-meter disposed in common case. Automaton-meter has measuring hydraulic cylinder. Floating piston is placed inside cavity of hydraulic cylinder; piston has permanent magnet inside it. Control unit is electrically connected with piston position detectors, which are mounted onto side surface of hydraulic cylinder, and with electromagnet three-way valves mounted onto solution feeding and discharge lines. Piston position detectors are mounted in end points along its movement onto guiding plank of side surface of hydraulic cylinder and they are provided with fastening lock screw.

EFFECT: improved efficiency of production of neutral anolyte solution; improved productivity; ability of controlling productivity and amount of doses of anolyte solution.

3 dwg

FIELD: methods and equipment for sterilizing of liquids and surfaces contacting therewith.

SUBSTANCE: method involves heating liquid to temperature lower than temperature desirable for sterilization by pasteurization which is provided by applying electric field with intensity of 102 V/cm and higher, with liquid or solid object being exposed to acoustic vibration in the process of applying of electric field. Apparatus has liquid product transportation system and sterilization station positioned along said system. Sterilization system comprises electric power source which creates in liquid passed through sterilization station electric field of intensity ranging between 102 V/cm and 104 V/cm, and ultrasonic generator.

EFFECT: increased efficiency in sterilization of liquid product and surfaces contacting therewith.

31 cl, 12 dwg, 11 tbl, 13 ex

Electrolyzer // 2252921

FIELD: medical instrument making, namely apparatuses for preparing ecologically safe electrically activated water.

SUBSTANCE: electrolyzer includes two electrodes (one electrode of stainless steel and other electrode of carbon); bridge type voltage rectifier; electric circuit plug; membrane; second doubled membrane - cover, for example of tracing paper or canvas; glass vessel; low-resistance voltage divider with taps; array of light emitting diodes with additional resistors whose number corresponds to that of taps; housing of electrolyzer. Plug is connected with inlets of bridge type voltage rectifier; negative outlet of voltage rectifier is connected with electrode of stainless steel. Second positive-polarity outlet of voltage rectifier is connected through low-resistance voltage divider with taps to carbon electrode arranged in second membrane-cover. All cathode ends of light emitting diodes with additional resistors are connected in parallel and they are connected with positive outlet of voltage rectifier; second ends of said diodes are connected with respective taps of low-resistance voltage divider.

EFFECT: possibility for displaying information concerning activation degree of water at preparing it for medical purposes, preparation of ecologically safe anolyte.

2 dwg

Antiviral agent // 2496522

FIELD: medicine, pharmaceutics.

SUBSTANCE: group of inventions discloses an antiviral agent (versions) able to inactive a virus. The antiviral agent characterised by containing the basic substance, cuprous microparticles, and inorganic microparticles to fix the cuprous microparticles on the basic substance. The inorganic microparticles are coupled together by chemical bonds formed between silane monomers found on the surface, and each of the inorganic microparticles is coupled with the basic substance by a chemical bond between silane monomer and the basic substance for the purpose of forming the areas wherein the above cuprous microparticles are expected to be accumulated.

EFFECT: group of inventions provides high antiviral activity.

16 cl, 3 tbl, 7 ex

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