Method to produce biomass of aerobic microorganisms

FIELD: biotechnologies.

SUBSTANCE: method includes inspection of an inoculator with process equipment for tightness, sterilisation of the inoculator with steam via an aeration device at the pressure of 0.20 - 0.25 MPa for 30…40 min., its filling with nutrient medium heated by steam to the temperature of 100°C. Then the temperature of the nutrient medium is increased to 121 - 123°C at steam pressure of 0.10 - 0.15 MPa, and the nutrient medium is maintained at these parameters for 15-60 min., afterwards the pressure in the inoculator is reduced down to 0.03…0.05 MPa. The nutrient medium is cooled down to cultivation temperature of 31…32°C with cold water with temperature of 7 - 10°C. After cooling of the nutrient medium, it is seeded with a seeding material with simultaneous mixing and aeration with sterile air. Cultivation of the produced liquid seeding culture is carried out at pH 4.2 - 4.5 and temperature of 31 - 32°C to achieve the phase of exponential growth for 12 - 14 hours. Then it is sent by means of displacement with sterile air from the inoculator into the prepared fermenter in the amount of 3…10% of the nutrient medium amount with its filling by 7/10 of its volume, and the microorganism culture is grown at fermentation temperature of 28 - 40°C for 96 - 120 hours with continuous aeration with sterile air, mechanical mixing and supply of warm water with temperature of 27 - 47°C into a heating jacket of the fermenter. After fermentation the cultural fluid with accumulated biomass is supplied into previously sterilised collectors of finished culture.

EFFECT: increased yield of cultural liquid with accumulated biomass of aerobic microorganisms, reduced specific power inputs and provision of environmental safety at all stages of production.

1 dwg, 2 ex

 

The invention relates to the microbiological industry, and can be used in the process of deep aerobic fermentation in growing cultures of micro-organisms and the producers of enzymes.

Known methods of production of biomass of aerobic microorganisms [Fermentation apparatus for processes of microbiological synthesis / Aouina, Lasgorceix, Art, Weaponfire // M: Delhi print, 2005. - 191 S.], including the preparation of a liquid nutrient medium, inoculum and cultivation of microorganisms.

The disadvantage is that the known methods do not provide for the preparation of energy resources and their rational use in the cultivation of microorganisms and can not be efficiently implemented in terms of decentralized heating systems, when thermal energy is generated directly on the object of production, which is typical of the mini-productions and enterprises of small capacity. This eliminates the possibility of using low-temperature heat capacity, in particular waste heat of gas turbine plants and boiler units, which does not allow to fully solve the problems of energy saving.

The closest in technical essence and the achieved effect to the present invention is a method for the production of biomass aerobic, microor is Anisimov [U.S. Pat. Of the Russian Federation No. 2322488 C2, C12N 1/00, SM 1/00, Publ. 20.04.2008. Bull. No. 11, C], providing for the feeding of the culture liquid aerating agent, which is used as air, exhaust air and the culture fluid with the accumulated biomass.

The disadvantage is that in this way there is no training system energy resources, in particular "warm" and "cold" water in a closed thermodynamic cycle using steam jet refrigerating machines operating in heat pump mode, to stabilize the temperature regimes in the preparation of liquid seed culture in inoculator, with the direct cultivation of a culture of microorganisms in the fermenter and cooling ready culture in foster collections, which does not allow to consider the known method as energy-efficient, environmentally safe and provides a high yield of the culture fluid with the accumulated biomass.

An object of the invention is to increase the culture fluid with the accumulated biomass of aerobic microorganisms, reducing energy consumption and ensuring environmental safety at all stages of production.

To solve the technical problem of the invention, a method for production of biomass of aerobic microorganisms, characterized by those who, first verify the integrity of inoculator with the cooling jacket, with devices aeration and mixing, with lines of the exhaust sterile air and squeezing the liquid inoculum in the fermenter for deep aerobic fermentation, and then sterilize the dry inoculant steam through the device aeration under pressure...0,25 0,20 MPa for 30...40 min and filled with nutrient medium, which is heated by steam to a temperature of 100°C, raise the temperature of the nutrient medium to 121...123°C at vapor pressure...0,15 0,10 MPa and maintain it with these settings within 15...60 min, after which reduces the pressure in inoculator to 0.03...0.05 MPa, and a cooling jacket served "cold" water temperature 7...10°C and cooled medium to a temperature of cultivation 31...32°C, after cooling of the nutrient medium to stop the flow of sterile air in the dry inoculant, close the line exhaust sterile air, produce seeding nutrient medium seeds and ensure its uniformity through the device mixing with aeration with sterile air; the liquid seed culture grown at pH 4.2...4.5 and a temperature of 31...32°C until the exponential phase of growth in for 12...14 hours and then it is directed by squeezing sterile without the Hom of inoculator in a pre-tested for tightness and sterilized fermenter with a heating jacket, with devices aeration and mixing in the number 3...10% of the amount of the nutrient medium, and fill it at 7/10 of its volume and carry out the cultivation of a culture of the microorganism at a temperature of fermentation 28...40°C for 96...120 hours under continuous aeration with sterile air, mechanical stirring and the supply of warm water with a temperature of 27...47°C in the heating jacket of the fermenter; after fermentation culture liquid with the accumulated biomass is served in pre-sterilized collections of ready-made culture that supports its temperature level 8...10°C by the cooling system; for the preparation of "cold" and "warm" water use of the ejector refrigeration machine operating in heat pump mode, and comprising ejector, evaporator, hallodapini, condenser, thermostatic expansion valve, collection of wastewater, steam generator with electric heating elements and a safety valve, feed pump water to the steam generator, pump recirculation of refrigerant through hallodapini working in a closed thermodynamic cycle with a steam generator receive a working vapor and under a pressure of 0.05...0,06 MPa served in the nozzle of the ejector, involving ejected refrigerant vapors, which use water from the evaporator and to create below is Noah pressure 0,0009...of 0.001 MPa with a boiling point of the refrigerant 4...7°C; due to rezerwacji refrigerant through hallodapini get "cold" water temperature 7...10°C by recuperative heat exchange between the refrigerant and water and serve it in a cooling jacket inoculator and cooling system collections of ready-made culture; the heat of condensation that formed after the ejector vapor mixture of refrigerant and working of steam in the condenser, is used to produce warm water, which by recuperative heat exchange is heated to a temperature of 27...47°C and served in the heating jacket of the fermenter; part formed after the condenser water condensate is directed through the expansion valve into the evaporator for depositing in it the loss of water, and the excess part is removed from the closed cycle of the ejector refrigeration machines and together with waste water after inoculator, fermenter and collections of ready-made culture served in the collection of waste water, from which one part water is directed to replenish the loss of water in the steam generator, and the other part in two streams served in hallodapini and condenser steam jet refrigeration machine with the formation of a closed loop.

The technical result of the invention is to increase the culture fluid with the accumulated biomass of aerobic microorganisms, increasing energy efficiency and the oncologic safety of operations.

Figure 1 presents a scheme that implements the proposed method.

The diagram shows the fermenter 1 with a heating jacket 2, the mixing devices 3 and aeration 4; dry inoculant 5 with the cooling jacket 6, the mixing devices 7 and aeration 8; collections of ready-made culture 9 with the cooling system 10; the collection of waste water and condensate 11; steam generator 12 with electric heating elements 13 and the safety valve 14; the ejector 15; capacitor 16; thermostatic expansion valve 17; the evaporator 18; hallodapini 19; pumps 20, 21, 22, 23, 24; line material flow: 0.1 - sterile air; 0.2 - exhaust sterile air; 1.0 - cold water; 1.1 - waste water; 1.2 - recycling of refrigerant through hallodapini; 1.3 - water hallodapini; 1.4 - water in the condenser; 7.5 - warm water in the fermenter; 2.0 - working couple; 2.7 - pair in the dry inoculant and the fermenter; 2.2 - ejected vapor refrigerant; 2.3 - mix work and the ejected vapor; 2.4 - condensate; 2.5 - pressure relief; 3.1 - nutrient environment; 3.2 - seed; 3.3 - squeezing liquid seed culture; 3.4 - culture liquid with the accumulated biomass of aerobic microorganisms.

The method is as follows.

Technological cycle of production of biomass of anaerobic microorganisms begin with cooking liquid seed culture in Ino is ustore 5 with the cooling jacket 6, the mixing devices 7 (two - or three-tiered stirrer and aeration 8 (nozzles or bubblers).

After leak testing of sterile air supplied by line 0.1 pressure of 0.07 MPa, and soap foam, applied to the junction (cover, flanges, gaskets, valves, etc.), dry inoculant checked for leaks steam, supplied through lines 2.1 through the aeration device 8 at the closed line of exhaust sterile air 0.2. If there are gaps of air or steam and reducing the pressure in inoculator, eliminate defects, and again checks for tightness.

After leak testing the dry inoculant 5 sterilized by steam supplied through line 2.1 of the steam generator 12 through the aeration device 8 under pressure...0,25 0,20 MPa for 30...40 min, and then fill the nutrient medium on line 3.1, which is heated by steam to a temperature of 100°C when the outdoor exhaust line 0.2. After that close the line exhaust sterile air 0.2 and bring the temperature of the nutrient medium to 121...123°C at vapor pressure...0,15 0,10 MPa and incubated for 15...60 minutes, then reduce the pressure in inoculator to 0.03...0.05 MPa, and a cooling jacket 6 through line serves 1.0 "cold" water and cooled medium to a temperature of cultivation 28...40°C.

The village is e cooling of the nutrient medium to produce its sowing seeds through planting hole (not shown) in line 3.2. Before sowing stop the flow of sterile air in the dry inoculant on line 0.1 and simultaneously close the line exhaust sterile air 0.2. After planting resume the supply of sterile air, open line exhaust sterile air 0.2 and realize the growing culture of microorganisms.

The resulting liquid seed culture inoculate 5 cultivated at pH 4.2...4.5 and a temperature of 31...32°C until the exponential phase of growth within 12...14 hours, ensuring its uniformity through the mixing device 7.

After the time of cultivation liquid seed culture perelavlivaet sterile air through the line squeezing 3.3 from inoculator 5 pre-tested for tightness and sterilized fermenter 1 of 3...10% of the amount of the nutrient medium, which fills 7/10 its volume.

Check for leaks and sterilization of the fermenter perform similarly, as inoculator.

In the fermenter 1 realize the growing culture of the microorganism with the temperature of the fermentation 28...40°C for 96...120 hours under continuous aeration with sterile air through the aeration device 3, a mechanical stirring with a mixing device 4 and the flow of "warm" water line 1.3 in the heating jacket 2.

For the preparation of "cold" and "warm" water use of the ejector refrigeration machine operating in heat pump mode, consisting of the ejector 15; evaporator 18; hallodapini 19; capacitor 16; thermostatic expansion valve 17; the collection of waste water 11, the steam generator 12 with electroheater elements 13 and the safety valve 14; a pump supplying water to the steam generator 23; pump recirculation of refrigerant through hallodapini 20, operating in a closed thermodynamic cycle.

In the steam generator 12 through the electric heating elements 13 receive a working vapor and under a pressure of 0.05...0,06 MPa on line 2.0 is served in the nozzle of the ejector 15, involving on line 2.2 ejected refrigerant vapors, which use water from the evaporator 18, and to create a reduced pressure 0,0009...of 0.001 MPa with a boiling point of the refrigerant 4...7°C. due to rezerwacji refrigerant line 1.2 through hallodapini 19 get "cold" water temperature 7...10°C by recuperative heat exchange between the refrigerant and water is supplied through line 1.3 hallodapini 19 of the collector 11 by a pump 24.

The resulting "cold" water from hallodapini 19 on lines 1.0 is served in the cooling jacket 6 inoculator 5 and the cooling system 10 collections ready the th culture 9.

Formed after the ejector 15, the mixture of refrigerant vapor and a working steam line 2.3 is sent to the condenser 16. The condensation process is accompanied by release of heat, the heat of condensation in the condenser 16 is used to produce warm water through recuperative heat exchange between the water supplied from the collector 11 by pump 24 through line 1.4 in the condenser 16, and condensing vapors of the mixture in the condenser 16. Heated to a temperature of 27...47°C water is fed by line 7.5 in the heating jacket 2 of the fermenter 1.

Part formed after the capacitor 16 of the water condensate is directed through thermostatic expansion valve 17 in line 2.4 in the evaporator 18 to replenish it loss of water, and the excess part of the condensate is removed from the closed cycle of the ejector refrigeration machines and together with waste water after inoculator 5, fermentor 1 and collections of ready-made culture 9 serves on the lines 1.1 in the collection of waste water 11 from which part of the water is directed along the line of 7.2 to replenish the loss of water in the steam generator 12, and the other part on two threads 1.3 and 1.4 with the help of the pump 24 serves to hallodapini 19 and the condenser 16 the ejector refrigeration machine with the formation of a closed loop. By increasing the steam pressure in the steam generator actuates the safety valve, providing pressure relief.

Method for the production of biomass of aerobic microorganisms is carried out in the following sequence. First, cook the liquid seed culture inoculator in accordance with the production cycle (h):

washing and inspection apparatus1,0
check for tightness0,5
pressure testing and sterilization2,5
download nutrient medium0,5
sterilization of the nutrient medium1,5
cooling and seeding nutrient medium2,0
the cultivation liquid seed culture12...14
transfer supernatant in a fermenter0,5

The process of cultivation of microorganisms in the aerobic fermentor deep fermentation carried out with aeration with sterile air through the bubbler and stirring with a mechanical stirrer, which was dosed to the feed flow of the nutrient medium, inoculum (inoculum), p is oxidation of sulfhydryl air, "warm" water in the heating jacket for high-intensity mass and energy exchange of microbial cells inoculum with a nutrient medium due to the stabilization of process parameters on the level required for optimal development of producer and education of the target product. From the fermenter exhaust air, waste water and the culture fluid with the accumulated biomass.

The production cycle of the fermenter was (in part):

washing and inspection apparatus1,0
check for tightness0,5
pressure testing and sterilization1,5
filling nutrient medium4,0
culturing in the fermenter96...120
transfer the culture fluid
in the supply tank0,5

The fermentation process is carried out in a vertical fermenter firm "Sartorius Stedim Biotech" series BIOSTATc displacement of 100 litres, intended for the cultivation of microorganisms or cultures cleto is. Control of process parameters is provided by a microprocessor-based control system DCU (Digital Control Unit). To stabilize the temperature regimes in the preparation of liquid seed culture in inoculator, immediate growing culture of microorganisms in the fermenter and cooling ready culture in foster collections are preparing a "warm" and "cold" water using steam jet refrigerating machines operating in heat pump mode, with the technical characteristics:

cooling capacity, kW20
boiling point:
in the evaporator, °C4
in the steam generator, °C154
condensing temperature, °C127
the water temperature at condenser inlet, °C15
the coefficient of ejection4
the area of heat exchange surface
hallodapini, m2 8
the heat transfer coefficient of hallodapini, W/m2·°C92
the area of heat exchange surface of the condenser, m26
the heat transfer coefficient of the condenser, W/m2·°C49
the refrigerantwater

The design of the ejector refrigeration machine with no moving consumable items, thus ensuring trouble-free operation of the machine long cycles without direct service and thus minimized the volume of repairs, the cost and the need for spare parts and auxiliary materials.

Example 1

As the object of production used enzyme preparation inulinase received in-depth way using producer micromycete Aspergillus awamori 2250.

The dry inoculant with the cooling jacket, with devices aeration and mixing, with lines of the exhaust sterile air and squeezing the liquid inoculum in the fermenter for aerobic submerged culture is checked for leaks, then sterilize the dry inoculant steam under a pressure of 0.25 MPa for 40 minutes Zapovednik is both a nutrient medium, which is heated by steam to a temperature of 100°C, raise the temperature of the nutrient medium to 123°C at vapor pressure of 0.15 MPa and maintain it with these settings for 40 minutes, then reduce the pressure in inoculator to 0.05 MPa and cooled medium to a temperature of cultivation 31±0.5°C and produce seeding nutrient medium seeds. Liquid seed culture grown at pH 4.2 and a temperature of 31±0.5°C until the exponential phase of growth within 12 hours. Then it direct from inoculator in a pre-tested for tightness and sterilized fermenter at a rate of 4% of the amount of the nutrient medium, and fill it at 7/10 of its volume and carry out the cultivation of a culture of the microorganism at a temperature of fermentation 31±0,5°C for 96 hours under continuous aeration with sterile air, mechanical stirring. After fermentation culture liquid with the accumulated biomass is served in pre-sterilized collections of ready-made culture, which support its temperature at 8°C.

The maximum yield of the target product activity was achieved by the following mode of cultivation:

the nutrient medium composition, %:

the concentration of molasses5,0
the concentration of (NH4)2HPO41,0
the concentration of MGSO4·7H2O0,05
concentration KH2PO40,1
the pressure of sterile air when submitting
in the fermenter, MPa0,03
the rotational speed of the stirrer, with-13,5
the pH of the liquid phase4,2
the cultivation temperature, °C31±0,5
the solids content in the culture fluid, %.7,0±0,5
activity inulinase, u/cm325±3
the activity of β-fructofuranosidase, u/cm3100±5
specific activity, u/g protein1200
the duration of fermentation, h96

Example # 2

The method was carried out analogously to example 1, N. the deep aerobic cultivation is carried out micromycete Trichoderma harzianum F114 producer of the enzyme β-mannanase, which hydrolyzes the mannans carbohydrate-containing vegetable raw materials to mannose.

The dry inoculant with the cooling jacket, with devices aeration and mixing, with lines of the exhaust sterile air and squeezing the liquid inoculum in the fermenter for aerobic submerged culture is checked for leaks, then sterilize the dry inoculant vapor pressure of 0.20 MPa for 30 minutes to Fill a nutrient medium, which is heated by steam to a temperature of 100°C, raise the temperature of the nutrient medium to 121°C at a pressure of 0.10 MPa and maintain it with these settings for 40 minutes, then reduce the pressure in inoculator to 0.03 MPa and cooled medium to a temperature of cultivation 35±0,5°C and produce seeding nutrient medium seeds. Liquid seed culture grown at pH 4.5 and a temperature of 35±0.5°C until the exponential phase of growth for 14 hours. Then it direct from inoculator in a pre-tested for tightness and sterilized fermenter in the amount of 2% of the amount of the nutrient medium, and fill it at 7/10 of its volume and carry out the cultivation of a culture of the microorganism at a temperature of fermentation 35±0.5°C for 72 hours under continuous aeration with sterile air, mechanical peremeshivaniem fermentation culture liquid with the accumulated biomass is served in pre-sterilized collections of ready-made culture, in which maintain its temperature at 10°C.

The maximum yield of the target product activity was achieved by the following mode of cultivation:

the nutrient medium composition, %:

glucose40,0
MgSO40,5
NaNO34,0
KH2PO41,0
KCl0,05
FeSO40,1
the pressure of sterile air
when filing in the fermenter, MPa0,04
the rotational speed of the stirrer, with-13,6
the pH of the liquid phase4,5
the cultivation temperature, °C35±0,5
the solids content in
the culture fluid, %7,0±0,5
the asset is ity β-mannanase, u/cm32400±5
specific activity, u/g protein11800
the duration of fermentation, h72

Rational use of heat and electric energy in the system cooling and heating with the use of the ejector refrigeration machine operating in heat pump mode, considered from the point of view of increasing the output of the culture fluid with the accumulated biomass of aerobic microorganisms and reduce the cost of the obtained target product.

The stabilization temperature processes of preparing a liquid seed culture and deep aerobic fermentation by reducing the temperature spread of energy, which was used to "warm" and "cold" water in a closed thermodynamic cycle with the use of the ejector refrigeration machine, allows to increase the output of the culture fluid with the accumulated biomass of aerobic microorganisms.

The basic fundamental solution to reduce energy consumption in the proposed method of production of biomass of aerobic microorganisms is the optimal choice of temperature changes in the evaporator and condenser steam jet refrigeration machine when receive the NII "cold" and "warm" water. Deviation from these values will inevitably lead to an increase in consumption of energy: the lower the boiling temperature of the refrigerant in the evaporator 1°C will result in the need to increase the flow of steam to the ejector, and consequently a loss of power for 5...7%, while the increase in condensing temperature by 1°C will lead to increased energy consumption 2,0...2,5% [Thermal and structural calculations refrigeration machines / Ammoboxes, Nay, Ederation and others; Under the General Ed. Iascone. - L.: Engineering. Leningrad branch, 1987. - 423 S.].

The proposed method for the production of biomass of aerobic microorganisms with the use of the ejector refrigeration machine expands the boundaries of energy efficient pairing of objects of different temperature potentials based on the recycling and recovery of waste energy. When fully implemented universal approach in the creation of a competitive technology for the generation of heat and cold together for processes of preparing a liquid seed culture in inoculator, immediate growing culture of microorganisms in the fermenter and cooling ready culture in foster collections.

Thus, the proposed method for the production of biomass of aerobic microorganisms allows you to:

- to increase the output of the culture liquid is accumulated biomass of aerobic microorganisms;

- to ensure environmental safety at all stages of production culture fluid with the accumulated biomass of aerobic microorganisms;

- allows you to reduce the specific energy consumption for 5...7% by rational enable inoculator, fermenter and collections of ready-made culture in heat production using steam jet refrigerating machines operating in heat pump mode;

to ensure the energy efficiency of fermentation processes due to the use of the heat of condensation of the refrigerant in the condenser of the refrigerating machine when water is heated and its subsequent submission to the heating jacket of the fermenter and capacity of the refrigerant in headpiece when the cooling water with the subsequent flow in the cooling jacket inoculator and cooling system receiving collections of ready-made culture;

- to create real conditions for utilization of low-pressure steam;

- as an energy carrier is used, the water vapor pressure of 0,05 0,06...MPa, resulting in savings of electricity, which is consumed only on the operation of controls, pumps refrigerant and water, electroheater elements of the steam generator.

Method for the production of biomass of aerobic microorganisms, characterized in that the first stateful tightness Ino is ulator with the cooling jacket, with devices aeration and mixing, with lines of the exhaust sterile air and squeezing the liquid inoculum in the fermenter for deep aerobic fermentation, and then sterilize the dry inoculant steam through the aeration device under pressure of 0.20 - 0.25 MPa for 30-40 min and filled with nutrient medium, which is heated by steam to a temperature of 100°C, raise the temperature of the nutrient medium to 121-123°C at vapor pressure of 0.10-0.15 MPa and maintain it with these settings within 15-60 minutes, then reduce the pressure in inoculator to 0.03-0.05 MPa, and a cooling jacket serves cold water with a temperature of 7-10°C and cooled medium to a temperature of cultivation 31-32°C, after cooling of the nutrient medium to stop the flow of sterile air in the dry inoculant, close the line exhaust sterile air, produce seeding nutrient medium seeds and ensure its uniformity through the device mixing with aeration with sterile air, the liquid seed culture grown at pH 4.2-4.5 and a temperature of 31-32°C until the exponential phase of growth for 12-14 h and then it is directed by squeezing sterile air from inoculator in a pre-tested for tightness and sterilized fermenter with abugre the surrounding shirt with devices aeration and mixing in the amount of 3-10% of the amount of the nutrient medium, and fill it at 7/10 of its volume and carry out the cultivation of a culture of the microorganism at a temperature of fermentation 28-40°C for 96-120 h under continuous aeration with sterile air, mechanical stirring and the warm water temperature 27-47°C in the heating jacket of the fermenter after fermentation culture liquid with the accumulated biomass is served in pre-sterilized collections of ready-made culture, which support its temperature at 8-10°C. by the cooling system, and for the preparation of cold and warm water using steam jet refrigerating machine running in heat pump mode, and comprising ejector, evaporator, hallodapini, condenser, thermostatic expansion valve, collection of wastewater, steam generator with electric heating elements and a safety valve, feed pump water to the steam generator, pump recirculation of refrigerant through hallodapini working in a closed thermodynamic cycle, thus obtained in the steam process steam under pressure 0,05 0,06...IPA served in the nozzle of the ejector with the involvement of the ejected vapor refrigerant, which use water from the evaporator, and create it decreased the pressure 0,0009-of 0.001 MPa with a boiling point of the refrigerant 4-7°C, due to rezerwacji refrigerant through hallodapini get cold water with a temperature of 7-10°C by recuperative heat exchange between the refrigerant and water and serve it in a cooling jacket inoculator and cooling system collections of ready-made culture, and the heat of condensation that formed after the ejector vapor mixture of refrigerant and working of steam in the condenser, is used to produce warm water, which by recuperative heat exchange is heated to a temperature 27-47°C and served in the heating jacket of the fermenter, the part formed by the condenser water condensate is directed through the expansion valve into the evaporator for depositing in it the loss of water, and the excess part is removed from the closed loop the ejector refrigeration machines and together with waste water after inoculator, fermenter and collections of ready-made culture served in the collection of waste water, from which one part water is directed to replenish the loss of water in the steam generator, and the other part in two streams served in hallodapini and condenser steam jet refrigeration machine with the formation of a closed loop.



 

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15 cl, 5 dwg, 2 tbl, 5 ex

FIELD: chemistry.

SUBSTANCE: invention relates to biochemistry. Disclosed is a method of delaying plant development processes, associated with ethylene biosynthesis involving exposing a plant or plant part to one or more bacteria which produce one or more enzymes selected from a group consisting of nitrile hydratase, amidase, asparaginase, and mixtures thereof and said one or more bacteria are selected from a group consisting of Rhodococcus, Brevibacterium ketoglutamicum, and mixtures thereof, and wherein said one or more bacteria act on the plant or plant part in an amount sufficient to delay the plant development process. Disclosed is an apparatus for delaying the plant development process associated with ethylene biosynthesis having several layers. At least one of the layers contains a catalyst which contains one or more bacteria selected from a group consisting of Rhodococcus, Pseudomonas chloroaphis, Brevibacterium ketoglutamicum and mixtures thereof, wherein the one or more bacteria produce one or more enzymes selected from a group consisting of nitrile hydratase, amidase, asparaginase and mixtures thereof.

EFFECT: invention increases efficiency of delaying plant development process associated with ethylene biosynthesis.

59 cl, 4 dwg, 9 tbl, 5 ex

FIELD: chemistry.

SUBSTANCE: enzyme preparations are obtained using a fermenter with a heating jacket for submerged culturing of microorganisms of the enzyme preparations with continuous aeration with compressed air and mechanical agitation at culturing temperature of 30…32°C on the entire volume of the fermenter; the culture liquid obtained in the fermenter is cultured to remove the residue and the filtrate of the culture liquid with moisture content of 92…95% is fed into a vacuum-sublimation drier, in which the desublimator used is a two-section evaporator of a vapour compression refrigerating machine, the working and spare section of which alternately operate in condensation and regeneration modes, respectively; wherein "hot" water is obtained by heating thereof in the condenser of the refrigeration machine using condensation heat of the coolant to temperature of 68…73°C, one part of which is fed into the heating jacket of the fermenter and the other is first fed for thawing the evaporator section operating in regeneration mode, and then the water cooled to temperature of 5…7°C is then removed from the evaporator section into a storage tank together with the liquid formed from the ice cover thawed on the surface of the cooling element in an amount of moisture evaporated from the enzyme preparation, and further, in closed cycle mode, fed in two streams, one of which is mixed with waste water after the fermenter before the condenser, and the second with "hot" water before the fermenter, wherein excess water from the recirculation loop is removed through the storage tank, followed by measurement of the culturing temperature in the fermenter, the flow rate and temperature of the water at the inlet of the heating jacket of the fermenter, the flow rate and moisture content of the filtrate of the culture liquid, residual pressure in the working volume of the vacuum-sublimation drier, the moisture content of the dried enzyme preparation, the flow rate and temperature of water vapour removed from the vacuum-sublimation drier into the working section of the evaporator, the boiling point of the coolant in the working section of the evaporator, the temperature of uncondensed vapour at the outlet of the working section of the evaporator and the level of water in the storage container; mass and heat flow of the mixture of cooled and "hot" water into the heating jacket of the fermenter is established by changing the ratio of flow rates thereof with adjustment of the culturing temperature in the fermenter; the measured values of moisture content and flow rate of the filtrate of the culture liquid are used to determine the power of the drive of the compressor of the refrigeration machine and the required residual pressure in the working volume of the sublimation drier by action on the power of the controlled drive of the vacuum pump with adjustment of the residual pressure on the final moisture content of the enzyme preparation; the flow rate and temperature of water vapour removed from the vacuum-sublimation drier into the working section of the evaporator, the temperature of uncondensed vapour at the outlet of the working section of the evaporator and the boiling point of the coolant in the working section of the evaporator are used to determine the current value of the coefficient of heat transfer from the water vapour to the coolant on the cooled surface of the working section of the evaporator, and upon achieving minimum permissible value of the coefficient of heat transfer, power of the drive of the compressor of the refrigeration machine is increased first, and the working section of the evaporator is then switched from condensation mode to regeneration mode while simultaneously switching to condensation mode the section operating in regeneration mode.

EFFECT: improved quality of enzyme preparations by increasing accuracy and reliability of controlling the process parameters, high energy efficiency and environmental safety of production processes and vacuum-sublimation drying.

1 dwg

FIELD: chemistry.

SUBSTANCE: when culturing phototrophs, the culture fluid is stirred and aerated through agitation by moving cultivators back and forth in the horizontal plane at given temperature and pH values. The cultivators are illuminated with a pulsed light source with pulse duration of 0.00001-0.001 s and pulse spacing of 0.01-0.1 s. In the apparatus used, the culture fluid is illuminated with diodes located under transparent bottoms of vessels of the same geometric shape and powered by a pulse generator with controlled frequency and light pulse duration.

EFFECT: group of inventions enables to reduce power consumption when culturing phototroph biomass.

2 cl, 2 dwg, 2 ex

FIELD: chemistry; biochemistry.

SUBSTANCE: device for speeding up reproduction, through faster reproduction and/or increased reproduction output of living cells in a suspension or any cultured organisms through a natural selection process has a flexible sterile pipe 7 with culture medium. A system of movable clamping apparatus 3, 4, 5 divides the pipe 7 into separate zones, containing spent culture (downstream zone), growing culture (growth compartment) and fresh growth medium (upstream zone). In the device there is an apparatus 13 for moving gates and the pipe such that, part of the growth compartment and the culture associated with it can be shut off by clamping apparatus and separated from the growth compartment. That way, part of the pipe which contains unused medium can be linked with part of the culture and medium associated with it, already present in the growth compartment.

EFFECT: realising a method with high reproduction output of living cells or cultured organisms.

36 cl, 10 dwg

FIELD: biochemistry, in particular, methods and devices for producing coloring substances, possible use in food and cosmetic industry, and also during various biological research.

SUBSTANCE: phycoerythrin protein pigment is produced by extraction from seaweed. It is extracted from seaweed, selected from a group including Galaxaura oblongata, Halymenia ceylanica, Helminthocladia australis and Porphyra dentate.

EFFECT: phycoerythrin has high optical density.

2 cl, 27 dwg, 2 tbl

FIELD: equipment for growing plant tissues.

SUBSTANCE: in accordance to the invention, unit for accelerating growth of plant tissues contains a set of boards, forming matrices of holes. Each hole contains a tissue sample. Support for boards is provided by a rack which contains a set of vertically stacked shelves, containing one or more holding recesses, which forcedly move boards to given positions. Light for tissue samples is provided by a set of matrices of light diodes, mounted on mounting plates. Light diodes emit white light. Each mounting plate is supported by corresponding end comber-type rack connector, so that light diodes are close to boards, supported by shelves, positioned lower. Matrix of light diodes preferably matches matrix of holes, supported by a lower positioned shelf in fixed position, so that each light diode is centered above a corresponding hole.

EFFECT: creation of high capacity system for processing samples of tissues which require light for supporting cell reproduction.

7 cl, 7 dwg

FIELD: biotechnology, in particular biopreparation production.

SUBSTANCE: claimed method includes feeding of sterilized broth into presterilized inoculator or bioreactor equipped with means for redox-potential (eH) controlling, including eH electrode and microprocessor unit for controlling and adjustment of eH and pH measurement of redox potential value for 1 h under stirring and comparison of steady-state eH values with steady-state values. When redox-potential value deviates from steady-state value of broth redox-potential by 10 % said broth is recognized as non-sterile one.

EFFECT: process of decreased cost.

4 tbl, 4 ex

The invention relates to the field of purification of gases and can be used for exhaust gas cleaning in microbiological, paint, chemical, food, petroleum refining, and processing of agricultural products

The invention relates to biotechnology, namely biotechnologische equipment used in the processes of cultivation of microorganisms

FIELD: biotechnologies.

SUBSTANCE: effluent treatment device comprises at least two baths (1, 2). Baths are connected to each other at the top and/or at the bottom by one or several connections (3, 4). One connection (4) is designed between areas of gas storage in the baths (1, 2). At least one of the baths contains a zone of gas storage (a), a zone of effluent treatment (c), comprising a fixed or movable substrate (10) to attach bacteria, a zone of effluent storage (b) and a decantation zone (d), a line (11) for drainage of sediments. The line (11) is functionally connected to the bottom of the baths (1, 2). The line (12) is designed for removal of gas from the device and is functionally connected to the top of the baths (1, 2). On the functional connection (3) between zones of decantation and the specified baths (1, 2) there is an isolating valve (5) and/or a control valve (6). On the line (4), which functionally connects the baths 1 and 2, there is a recirculation pump (7) for recirculation of fluid between baths and creation of level imbalance between baths. Baths are made so that the zone of gas storage (a) is above the zone of effluent treatment (b).

EFFECT: group of inventions makes it possible to provide for efficient monitoring of biofilm thickness, creation and preservation of optimal medium for development and activity of anaerobic bacteria.

12 cl, 4 dwg

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