Bioreactor and method of culturing photosynthesising microorganisms using said bioreactor

FIELD: chemistry.

SUBSTANCE: bioreactor has a vessel (1) with a cover (2) and a device for mixing and aerating microorganisms, having pipes (3 and 4) on the cover for inlet of aerating gas and outlet of gaseous medium, respectively. The vessel (1) is fitted with multiple coaxially arranged and spaced apart annular partitions (12 and 13) with floats (14 and 15) which are open at the bottom, on a vertical hollow shaft (11) with possibility of rotation and back-and-forth movement thereon with formation of a gap between the wall of the vessel (1) and the annular partitions (12 and 13). The vessel (1) and the cover (2) of the reactor are made from transparent materials. The bioreactor has a meant (19) of holding the reactor afloat in a liquid medium and sources (20 and 21) of artificial light, which are mounted inside the floats (14 and 15) of the annular partitions (12 and 13). The latter are made from optically transparent material. The vessel is in form of a disposable or reusable dismountable envelope (22) and has means (23 and 24) of fastening it to the cover (2) and the bottom (17) of the vessel, respectively. The method of culturing photosynthesising microorganisms involves creating biochemical and physical conditions for growth of the microorganisms in a culture medium and maintaining culturing temperature conditions. The latter is carried out in the bioreactor, which is immersed and held afloat in a natural or manmade water body at water temperature which is favourable for growth of photosynthesising microorganisms in said water body, wherein the culture medium used is the filtered water from the water body in which the bioreactor is located.

EFFECT: high output of biomass of photosynthesising microorganisms, while reducing power consumption and simplifying maintenance.

6 cl, 1 dwg

 

The invention relates to a device for growing single-celled microorganisms such as green algae, in closed containers in a water suspension under natural or artificial light. The invention can find application in the biotechnology industry for "biofuels", pharmaceutics, Microbiology, food industry and agriculture.

Known cultivator of microalgae, which with the help of pump suspension of Chlorella or spirulina served in the spray where it is divided into the drip stream, flows into illuminated from below by light sources (USSR author's certificate No. 1289880, IPC SM 1/04,publ. 1987).

However, the cultivator because of the obvious foaming and fouling light box light source lighting culture is ineffective, and mechanical connections of the pump, spray destroyed cells grown culture.

Known bioreactor for photosynthesis of green algae inside the tank which has a water-cooled xenon lamp and mixing device with blades [German Patent No. 2502515, IPC A01G 33/00, publ. 20.11.1975,]. Bulb immersed in liquid culture and periodically irradiates it with opaque rotating cylinder with a longitudinal slot, put on the lamp. Another option is m this solution is periodic intermittent turn on the lamp.

However, this device provides insufficient biomass yield of microorganisms due to uneven mixing environment cultivation with a mechanical stirrer, a traumatized cells specified stirrer and inefficient aeration cells carbon dioxide. In addition, this reactor irrational uses a light source, decreasing the efficiency of irradiation with intermittent inclusion and reduced lamp life. And if the bearings are immersed in liquid culture, happen to damage and destroy cells of the crop, resulting in growing forms with weak cell membranes.

Known bioreactor for the cultivation of photosynthetic microorganisms (RF Patent No. 2035505, IPC SM 1/00, publ. 20.05.1995 g)containing capacity with heat exchanger, the supply of nutrients and discharge of the finished product, water-cooled light source placed in the cavity formed by two coaxial disks connected to each other at the periphery of the blades, and the lower disk provided with a hollow inverted Cup in the cavity of which is placed lower bearing mixing device, the rotation of which under the action of centrifugal forces liquid culture, up the walls of the bioreactor, forms a hydraulic funnel, into the cavity of the cat who Roy is the light source, thus deprived of contact with liquid culture. As the cooling water temperature is lower than the temperature of the liquid culture, the light source happen condensation of moisture vapour and dripping down, i.e. there is a constant erosion of the surface light source, eliminating the fouling micro-organisms. While in the funnel suspension is intensively mixed, the rapid release of metabolic gas from it and its saturation nutrient gas mixture due to the increased surface phase contact suspension and good circulation in the boundary layer at the effective irradiation formed in a funnel. At the same time due to the condensation of water vapor on the water-cooled flask sharply reduced the removal of water vapor from exiting the gas phase of the bioreactor. The placement of the lower support mixing device inside the inverted blank glass eliminates mechanical damage to cells grown culture, since the bearing supports mixing device during the lling of liquid is in the air tube, which is formed in the glass.

However, this device also provides insufficient biomass yield of microorganisms due to uneven mixing environment cultivation with a mechanical stirrer, a traumatized cells specified mesalc the th and inefficient aeration cells with carbon dioxide.

The closest analogue (prototype bioreactor is for mixing and aeration, including for the cultivation of photosynthetic microorganisms (RF patent No. 2299903, IPC SM 1/04, publ. 27.05.2007, containing a container with a lid and a device for mixing and aeration of microorganisms, including nozzles, respectively, for supply of aerating gas and exhaust of gaseous medium, placed in the lid of the container, and configured to generate on the surface of the suspension swirling flow of aerating gas field velocity potential vortex at the periphery of the vessel and the axial flow in the paraxial area and the differential pressure between the periphery and center of the vortex and the annular wall. The annular wall has an open bottom floats and installed in the vessel on the vertical axis for rotation and reciprocating her movement and axial capacity with the formation of a gap between the cylindrical vessel wall and the annular wall.

The disadvantage of this bioreactor is insufficient biomass yield of microorganisms when the ratio of the height of the vessel to the diameter of the reactor is more than 1.0 due to the significant non-uniformity of illumination of the cells of microorganisms in different zones of the reactor vessel, especially toward the bottom. In the case of external light source transparent is E. the vessel wall will be overgrown with biomass photosynthesising microorganisms, what complicates the process of maintenance of the reactor. In addition, the bioreactor is not adapted for the cultivation of marine or lacustrine photosynthetic algae directly in terms of immersion of the device in the reservoir due to the lack of means to ensure the buoyancy of the bioreactor.

The closest analogue (prototype) method of cultivation is the method of cultivation of photosynthetic microorganisms, including temperature cultivation via a heat exchanger, the saturation of carbon dioxide suspension photosynthetic microorganisms and maintaining the maximum value of the intensity of photosynthesis of microorganisms by changing the intensity of the saturation of the suspension of microorganisms by carbon dioxide regulation of the flow rate of the injected gas containing carbon dioxide (RF patent No. 2019565, IPC C12Q 3/00, publ. 15.09.1994 year).

However, in this method, significant costs are energy costs on the operation of the cultivation of photosynthetic microorganisms in a certain temperature range, which increases the cost of the final product.

The technical result of the invention is the creation of such a bioreactor and method of cultivation of photosynthetic microorganisms, which would increase the biomass yield of Potosi desiroush microorganisms and would reduce energy costs for obtaining biomass of microorganisms by culturing these microorganisms in the reactor directly in a natural or artificial pond, and also simplify the maintenance process of fermentation equipment.

This technical result is achieved by the fact that in the bioreactor for cultivation of photosynthetic microorganisms containing a container with a lid and a device for mixing and aeration of microorganisms, including nozzles, respectively, for supply of aerating gas and exhaust of gaseous medium, placed in the lid of the container, and configured to generate on the surface of the suspension swirling flow of aerating gas field velocity potential vortex at the periphery of the vessel and the axial flow in the paraxial area and the differential pressure between the periphery and center of the vortex and an annular wall with an open bottom floats installed in the vessel on the vertical axis for rotation and reciprocating it displacement and axial capacity with the formation of a gap between the cylindrical vessel wall and the annular wall, according to the invention it is equipped with a second or several annular partitions, made and installed on the same vertical axis is identical to the first annular wall at a distance from each other, while the lid and the capacity of the reactor is made of translucent materials.

Bioreactor equipped with a means to keep the reactor in a liquid medium on plaussible to keep the reactor in a liquid medium afloat made in the form of one or more floats, attached to the top of the silo. The bioreactor is equipped with artificial light sources installed in the cavities of floats annular walls made of optically transparent material.

Cover the container has a rigid construction, and capacity made in the form of a disposable or reusable removable shell and provided with means for securing it to the lid of the reactor vessel.

The shell of the tank is made of soft optically transparent elastic polymer material, or of soft permeable woven or non-woven material.

This technical result is also achieved by the fact that in the method of cultivation of photosynthetic microorganisms by creating biochemical and physical conditions for their growth in a nutrient medium in the bioreactor, including the temperature of the cultivation of these microorganisms according to the invention the temperature of the process of cultivation of photosynthetic microorganisms is carried out by immersion and maintain in a floating condition of the bioreactor in a natural or artificial reservoir when the water temperature to ensure proper growth of photosynthetic microorganisms in the specified reservoir, and as a nutrient medium use filtered water specified what about the reservoir, where is the bioreactor.

The temperature of the water in a natural or artificial reservoir may be +4.0 to+98°C.

It is found experimentally that when the incomplete lling of the bioreactor (for example, in the case of plum harvest cells by continuous technological cycle of cultivation) or increase the speed of rotation of the bioreactor gas vortex over 1800 rpm, decreasing the efficiency of mixing in the presence of one floating ring partitions, since the latter is pushed to the surface of the culture fluid, where again gets axial resistance and closes the flow of the culture fluid. In the inventive bioreactor with multiple annular washers, by intensive mixing of the culture fluid (speed bioreactor gas vortex over 1800 rpm) through the bottom (lower) annular washers in the case of locking of the flow of the liquid upper annular partition.

In the prior art such a constructive reception is not known.

The reduction of energy consumption is achieved by using the temperature of the water natural water body (sea, lake, ocean) to maintain the temperature of the cultivation of these microorganisms.

Since the heat capacity of air is many times lower than the heat capacity of water, naznacite the performance communications the temperature of the air will not have a significant effect on decreasing water temperature.

It also assumes the use of a bioreactor in the Equatorial zone with a constant year-round temperature.

Sudden changes in temperature of fluid in the device developed at night, as the layer of air under the hood is a good heat insulator, and the body is in water that is cooled gradually.

Maintaining a temperature in the range of +4 to+98°C provided the temperature of the water natural water source, the sea, lake, ocean), in which the cultivation of these microorganisms. For example, the hot springs in Kamchatka (+98°C) or the North sea (+4°C).

The proposed approach to the placement of the floating careaction in the waters of seas, lakes, etc. solves the problem of experience with low-cost biomass of microalgae, using readily available natural resources (sea, lake water as the culture fluid) and allows the use to create them cheap "soft" materials - polymer film, etc. the Casing is made of soft thin transparent material, may be removable, disposable and easily replaced by a new one, which simplifies its maintenance.

Figure 1 presents a diagram of a bioreactor for the cultivation of photosynthetic microorganisms.

The proposed method suspension ku is tipirovaniya cells of photosynthetic microorganisms is carried out as follows. Capacity 1 (figure 1) collected from individual parts and fill with sterile nutrient medium so that the height of the layer of the nutrient medium, provided the conditions for the cultivation of microorganisms, and the height of the layer of the gas environment would provide buoyancy to the reactor in the pond. The buoyancy of the bioreactor can be achieved also through external funds 19 made in the form of a pontoon. Then install in the tank 1 is required for cell cultivation of microorganisms temperature by immersing the reactor in a natural or artificial reservoir, for example in the summer favorable growth of photosynthetic microorganisms in the specified reservoir and enter the sowing dose of microorganism cells (Chlorella or spirulina, or another type). The water temperature in the reservoir depending on the type of photosynthetic microorganisms can be +4.0 to+98°C. Then the container 1 is injected aerating gas (air with the addition of CO2) and continuously stirred cell suspension. Mixing the cell suspension is carried out by its steady-state rotational motion generated by aerating gas, which is fed to the tank above the surface of the cell suspension while simultaneously twisting in the flow field velocity potential vortex at the periphery of the vessel and the axial flow in the paraxial zone. And ryuushi the gas is fed into the vessel at a selected pressure, providing a pressure drop in the swirling flow between periphery and center, for example, in the range 10-2000 PA.

The differential pressure between the periphery and the axis of the vortex determines the magnitude of the tangential components of the velocity of the gas stream above the surface of the cell suspension. When the differential pressure less than 10 PA, the velocity of the gas is low and it creates energy in the cell suspension will be insufficient to create the conditions for mixing, the resulting cells will settle to the bottom of the tank. When the differential pressure above 2000 PA starts capturing droplets of the suspension from the surface and their subsequent release into the vessel wall. This leads to massive cell death due to their mechanical injury.

As a result of intensive swirl flow of aerating gas above the surface of the cell suspension in the vortex is the pressure difference between its periphery and the center. The pressure difference in the gas through the free surface of the suspension cells generates in the last axial movement downward in the peripheral zone of the tank and upward in the axial zone. At the same time, due to friction of the gas on the free surface of a liquid medium, there is rotational movement of the suspension. In the process of cell culturing microorganisms aerating gas interacts with the cell suspension through its free surface, we is based on its mixture of air with CO 2and not mingling with her. As a result, the cell suspension eliminates the formation of gas bubbles, which reduces trauma to the cells and the formation of foam. As specified eddy stirring of the suspension is a quasi-stationary process, it is provided by the intensification of mass transfer characteristics. Operating time after a sufficient number of cells of the cell suspension merges and added fresh nutrient medium.

Immersion of the reactor in the reservoir delivers significant energy savings while obtaining the biomass of microorganisms.

Bioreactor, which carry out the method of cultivation of photosynthetic microorganisms, includes (1) an optically transparent cylindrical container 1, the cover 2 and the device for mixing and aeration of microorganisms, including the supply pipe 3 aerating gas and the pipe 4 exhaust gaseous environment, placed in the lid 2 of the container, and configured to generate on the surface of the suspension swirling flow of aerating gas field velocity potential vortex at the periphery of the vessel and the axial flow in the paraxial area and the differential pressure between the periphery and center of the vortex. In addition, the cover 2 is connected respectively to the nozzles 5 and 6 of incoming raw material supply and removal of the finished product of Mouth is eusto for mixing and aeration of the medium with the microorganism cells are made in the form of an annular cavity 7, formed by a cylindrical wall 8 that is installed in the lid 2. From the bottom to the cylindrical wall 8 is attached to the radial plate 9 with a gap relative to each other, forming a tangential slit 10 for feeding into the container 1 aerating gas tangentially to the walls of the container 1. The feed pipe 3 aerating gas is located on the periphery of the cover 2 and connected with the annular cavity 7, and the nozzle 4 is located on priosevoy area of the cover 2 and connected with the inner cavity of the container 1. In addition, the device for mixing and aeration of the medium with the cells of microorganisms contains a coaxially arranged on a hollow vertical axis 11 at a distance from each other, the annular walls 12 and 13, made of optically transparent material, installed in the vessel for rotation, forming with the container wall 1 a gap and provided with optically transparent hollow floats 14 and 15, open at the bottom and supporting walls 12 and 13 afloat in the liquid. Between the partitions 12 and 13 are connected by vertical posts 16. The lower end of the hollow axle 11 is attached to the bottom 17 of the container 1 and has a channel 18 for connecting the internal cavity axis 11 with a capacity of 1. The upper end of the hollow axle 11 is fixed in the cover 2 and is connected to the exhaust pipe 6 of the finished product. The bioreactor is equipped with means 19 for holding the reactor in a liquid medium on the square the Wu, made in the form of pontoons attached to the lid 2. The bioreactor is equipped with springs 20 and 21 of artificial lighting, mounted respectively in the cavities of floats 14 and 15 of the annular walls 12 and 13. Cover 2 and the bottom 17 of the tank have a rigid structure, and the side wall 22 of the container 1 can be made as a disposable or reusable removable soft shell, for example, of soft optically transparent elastic polymer material, or of soft permeable woven or non-woven material (e.g., acrylic) and provided with means 23 for securing it to the lid 2 of the container of the reactor and a means 24 for attaching it to the bottom 17 of the tank, made for example in the form of a bolted connection of their ends. Removable reusable shell simplify the process of preparation of the bioreactor to work, simplify the removal process (laundering surfactants) growths biomass of microorganisms and disposable sheaths are replaced with new ones.

Bioreactor operates as follows. Preferably the reactor is placed in water (littoral zone of a lake or sea) in the warm season, when the water temperature during the whole time of day varies about 27.0-32,0°C, so that does not require energy for thermal stabilization of the cultivation process. Collecting tank 1 by sakralen the edges of the wall 22, made in the form of a sleeve (transparent elastic membrane or semi-transparent shell of the non-woven material such as acrylic), respectively on the lid 2 and the bottom 17 by means 23 and 24, made in the form of clamps, the ends of which are drawn together by bolting. Into the container 1 in sterile conditions set on the axis 11 of the annular walls 12 and 13 and filled with nutrient medium, for example filtered sea water through the pipe 5 so that over the surface of the medium in the upper part of the vessel 1 with the lid 2 remained the cavity for movement of the aerating gas, the annular wall 12 with floats 14 were placed in a nutrient medium at its surface, and the annular wall 13 with floats 15 - in the middle part of the volume of the nutrient medium, for example as shown in figure 1. Next, enter the sowing dose of photosynthetic cells of microorganisms, such as Chlorella (for example, in an amount of 0.1 g per 1 liter of nutrient medium.), and include a drive motor (not shown in figure 1), which feeds through the pipe 3, the air, enriched, for example, carbon dioxide (Chlorella), in the annular cavity 7 and further through the slit 10 tangentially into the tank 1 and through the pipe 4 exhaust gas. Treatment system air carbon dioxide figure 1 is not shown. Depending on the technology requirements install the necessary number is on the speed of the impeller. Above the surface of the cell suspension vacuum is created in the axial zone of the tank 1 and the increased pressure on the periphery of this capacity. Under the action of pressure difference between the peripheral and the axial zone of the gas cavity above the surface of the cell suspension creates a swirling flow of gas that forms in the culture fluid turbulent rotational motion with intensive stirring along the vessel axis (field velocity potential vortex at the periphery of the vessel and the axial flow in the paraxial area and the differential pressure between the periphery and center of the vortex). The annular walls 12 and 13 rotate in the same direction and with the same angular speed as the culture fluid and kept afloat by means of floats 14 and 15. By establishing the capacity of several annular walls, the height of the container 1 may exceed its diameter by 3-4 times. In the cultivation process gas AERONET cells on the surface of the liquid. As a result of such aeration in the culture fluid are not formed gas bubbles and foam and does not injury the cells. The speed of the gas vortex (3-6 m/s) does not cause detachment of drops of the culture fluid from the surface, which also reduces trauma to the cells. The traffic is to increase the upward and downward flow of the cell suspension is achieved midrange is t a rotating annular walls 12 and 13 and to cultivate cells without stagnant zones at the height of the tank 1 environment equal to or several times (3-4 times) larger diameter of this vessel. It provides optimal conditions for the cultivation of photosynthetic microorganisms. The process is conducted continuously to increase the biomass of photosynthetic microorganisms such as algae Chlorella up to 15-20 g per 1 liter of the suspension, and then through the hollow shaft 11 and the pipe 6 is drained part of the suspension (the harvest), through pipe 5 add fresh medium and continue the process of cultivation. In the day time (a period of about 10-11 hours) cultivation are using natural lighting due to the fact that the reactor is in an open reservoir and the cover 2, the wall 22 of the vessel walls 12 and 13 and the floats 14 and 15 are made of optically transparent materials. After sunset (or in cloudy weather) include sources 20 and 21 of artificial lighting. Because the sources 20 and 21 of artificial lighting are in the gas cavities of floats 14 and 15, and the annular walls 12 and 13 with floats 14 and 15 are in continuous rotational motion, the above items are not subject to fouling by algae.

In such a constructive implementation of the bioreactor increased the biomass yield of photosynthetic microorganisms for improving the uniformity of illumination of the cells of the microorganisms, the possibilities under the Oia in larger containers, the height of which exceeds the diameter several times, and allows the cultivation of these microorganisms in the reactor directly into the reservoir, which significantly reduces the energy consumption for the process temperature of cultivation. Removable disposable or reusable transparent shell simplify the process of preparation of the bioreactor for use, and reusable shell is much easier to wash off growths biomass of microorganisms than when laundering permanent walls of the reactor.

The advantages of the proposed bioreactor method of cultivation of photosynthetic microorganisms over the known analogues are as follows: there was such a mass, which are not injured cells of microorganisms; efficient and uniform illumination of the entire volume of the suspension; minimum occlusion of the light transmitting surfaces and walls of the reactor; rapid replacement of the vessel wall of the reactor in case of fouling organisms; minimum foaming due gazovyhlopa mixing; the possibility of cultivation of different, including without shell forms photosynthetic microorganisms due to reduced traumatized cells; ability to produce large amounts of biomass; minimum power consumption by placing the reactor directly edstone in the pond.

Industrial applicability. The invention can be used in biotechnology, Microbiology, pharmacy and agriculture. Manufactured industrial designs of the proposed bioreactor reactor, which are used to implement the proposed method for the cultivation of photosynthetic microorganisms.

1. Bioreactor for the cultivation of photosynthetic microorganisms containing tank (1) with a lid (2) and device for mixing and aeration of microorganisms, including pipes (3), (4), respectively, for supply of aerating gas and exhaust of gaseous medium, placed in the lid (2) capacity, and ability to create on the surface of the suspension swirling flow of aerating gas field velocity potential vortex at the periphery of the vessel (1) and axial flow in the paraxial area and the differential pressure between the periphery and center of the vortex and the annular wall (12) with an open bottom hollow floats (14)installed in the vessel (1) coaxially her on the vertical axis (11) for rotation and reciprocating it move with the formation of the gap between the vessel wall (1) and an annular wall (12), characterized in that it is provided with a means (19) to keep afloat bioreactor in a liquid medium natural or artificial reservoir when the water temperature, ensure asiausa favorable growth of photosynthetic microorganisms, sources (20 and 21) artificial lighting mounted respectively in the cavities of floats (14 and 15) of the annular walls, and a second or several annular walls (13), made and installed on the same vertical axis (11) is identical to the first annular wall (12) at a distance from each other, and an annular wall (12 and 13) is made of optically transparent material and are connected by vertical struts (16)and cover (2) and the container (1) of the bioreactor is made of translucent material, and the cover (2) has rigid construction, and capacity (1) are designed as disposable or reusable removable sheath (22) and provided with means (23 and 24) for fastening respectively to the lid (2) and the bottom (17) of the capacity of the bioreactor.

2. The bioreactor according to claim 1, characterized in that the means (19) to keep the reactor in a liquid medium afloat made in the form of one or more pontoons attached to the lid (2) containers.

3. The bioreactor according to claim 1, characterized in that the shell (22) of the container (1) made of soft optically transparent elastic material.

4. The bioreactor according to claim 1, characterized in that the shell (22) of the container (1) made of soft permeable woven or non-woven material.

5. The method of cultivation of photosynthetic microorganisms by creating biochemic the ski and physical conditions for their growth in a nutrient medium in the bioreactor, including maintaining the temperature regime of the process of cultivation of these microorganisms, characterized in that the cultivation is carried out by means of a bioreactor according to claim 1, and temperature of the process of cultivation of photosynthetic microorganisms is carried out by immersion and maintain in a floating condition of the bioreactor in a natural or artificial reservoir when the water temperature to ensure proper growth of photosynthetic microorganisms in the specified reservoir, and as a nutrient medium use filtered water specified reservoir, which is located bioreactor.

6. The method of cultivation of claim 8, wherein the temperature of the water in a natural or artificial reservoir is 4.0-98°C.



 

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25 cl, 5 dwg

FIELD: medicine.

SUBSTANCE: venous blood filtration apparatus comprises a glass graduated cylinder 1 with an inlet 12 for venous blood supply and an outlet 13 for venous blood outflow, enclosed in a transparent plastic casing 2 with two tubes 3 and 4. The lower tube 4 is designed to deliver water heated to +36°C to the casing 2, while the upper tube 3 is used for air outlet from the casing 2. At the bottom of the glass graduated cylinder 1, there is a plastic grating 5 whereon a calibrated filter 7 of pore diametre equal to 5 mcm is fixed with a metal ring 6. The cylinder 1 accommodates an in-built piston 8 with a rod 9, and the piston has two channels 10 of different diametres on a junction of which there is a metal ball 11 for air discharge from the cylinder 1.

EFFECT: accelerated venous blood examination result with maintained precision of blood cancer cell detection, simplified designed of the related apparatus.

3 dwg

FIELD: medicine.

SUBSTANCE: said device comprises a reservoir with a calibrated orifice, and an air chamber connected to two compressors. The reservoir is connected to the first compressor through a pressure regulator, while a distal end of the reservoir is mounted in the air chamber and four-sided clamped with screws in a distal sector of the air chamber. The air chamber represents a receiver and is connected to the second compressor through the pressure regulator and a rotametre. The calibrated orifice of the reservoir is mounted in the air chamber and opposed to a nozzle in the chamber so that the axis of the calibrated orifice of the reservoir is aligned with the axis of the nozzle aperture. The device makes ensures making capsules of desired dimensions without damages, of uniform and smooth surface that provides their transplantation through an injection needle.

EFFECT: improved viability of encapsulated cells and tissues.

4 dwg

FIELD: microbiology instruments.

SUBSTANCE: invention relates to suspension cultivation of tissue or microorganism cells and can be used in food industry, biotechnologies and medicine. Proposed apparatus comprises cylindrical vessel with cover and device to aerate and mix suspension of mentioned biological substances. The said apparatus allows producing, on suspension surface, aerating gas swirled flow flowing along the vessel edges, potential vortex rate field being located at the vessel edges and axial countercurrent in near-axis zone. Note that the pressure difference between the vortex edges and centre varies from 10 to 2000 Pa. Axisymmetric body of revolution is arranged inside the said vessel, on its bottom and aligned with it, the body height being equal to or exceeding the level of the apparatus maximum filling. Side surface of aforesaid body of revolution smoothly mates the vessel bottom surface. Aforesaid body of revolution represents a cone or a cylinder with aerodynamic nose fairing arranged on its upper end face.

EFFECT: higher efficiency of cultivation due to more regular distribution of cless culture in fluid substrate.

2 cl, 4 dwg

FIELD: engines and pumps.

SUBSTANCE: invention relates to studying technological processes in heterogeneous media using microorganisms, particularly, in biohydrometallurgical production of noble metals. The proposed laboratory unit represents a bioreactor mounted between the top and bottom frames. The bioreactor cylindrical housing and cover are screwed together. The top frame accommodates a motor, belt transmission and bearing assembly, the bearing being spaced apart on the shaft. The bioreactor cover is rigidly attached to the top frame via the said bearing assembly. The cover houses a tubular aerator and pickups. The bioreactor housing accommodates a tubular heat exchanger, deflectors and mixer driven by the motor via the bearing assembly shaft.

EFFECT: expanded performances, higher reliability, lower costs and improved ergonomic properties.

1 dwg

FIELD: biology.

SUBSTANCE: present invention relates to devices for growing tissue cells and microorganisms in null-gravity conditions and can be used in space biotechnology. The device has a cylindrical container with a cover and a device for aerating and moving suspension of the said biological objects, comprising connecting pipes for inlet of aerating gas and outlet of gaseous medium respectively, fitted in the cover of the container, and made with possibility of creating on the surface of the suspension, rotational flow of aerating gas with velocity field of the potential vortex on the periphery of the container and an axial counter-flow in the paraxial area and a 10-2000 Pa pressure drop between the periphery and the centre of the vortex. The inner surface of the container and the bottom are made from material with limiting wetting angle θ=90°, or part of the inner surface on the side of the bottom and the bottom itself are made from material soaked with a liquid substrate with limiting wetting angle θ<90°. The other part of the inner surface of the container, on the side of its cover, is made from material not soaked with liquid substrate with limiting angle θ>90°, where in degrees are values of wetting angles of aqueous substrates on solid surfaces. The inner surface of the container which is not wet, on the side of the cover, can be made in form of a cylindrical insert with a holder, holding it in position, the outer diametre of which corresponds to the inner diametre of the container and is put into the container with possibility of moving along its axis. In an alternative of making the device, the inner surface of the container which is not wet, made in form of a cylindrical insert, is made from Teflon, and the rest of the soaked part of the surface of the container is made from stainless steel.

EFFECT: process of growing cells or microorganisms in microgravity conditions due to provision for stable conditions of the boundary surface between liquid and gaseous phases.

7 cl, 7 dwg, 3 tbl

FIELD: biology.

SUBSTANCE: present invention relates to devices for growing tissue cells and microorganisms in null-gravity conditions and can be used in space biotechnology. The device has a cylindrical container with a cover and a device for aerating and moving suspension of the said biological objects, comprising connecting pipes for inlet of aerating gas and outlet of gaseous medium respectively, fitted in the cover of the container, and made with possibility of creating on the surface of the suspension, rotational flow of aerating gas with velocity field of the potential vortex on the periphery of the container and an axial counter-flow in the paraxial area and a 10-2000 Pa pressure drop between the periphery and the centre of the vortex. The inner surface of the container and the bottom are made from material with limiting wetting angle θ=90°, or part of the inner surface on the side of the bottom and the bottom itself are made from material soaked with a liquid substrate with limiting wetting angle θ<90°. The other part of the inner surface of the container, on the side of its cover, is made from material not soaked with liquid substrate with limiting angle θ>90°, where in degrees are values of wetting angles of aqueous substrates on solid surfaces. The inner surface of the container which is not wet, on the side of the cover, can be made in form of a cylindrical insert with a holder, holding it in position, the outer diametre of which corresponds to the inner diametre of the container and is put into the container with possibility of moving along its axis. In an alternative of making the device, the inner surface of the container which is not wet, made in form of a cylindrical insert, is made from Teflon, and the rest of the soaked part of the surface of the container is made from stainless steel.

EFFECT: process of growing cells or microorganisms in microgravity conditions due to provision for stable conditions of the boundary surface between liquid and gaseous phases.

7 cl, 7 dwg, 3 tbl

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