Method for prediction of developing atopic diseases in newborns

FIELD: medicine.

SUBSTANCE: for the purpose of prediction of developing atopic dermatitis in newborns, umbilical blood plasma is examined for a level of interleukin-18. If the related level is 34 pg/ml or lower, developing atopic dermatitis in the newborn is predicted.

EFFECT: use of the given method enables well-timed evaluation of a risk of developing atopic dermatitis in newborns and early preventive measures for prevention thereof.

2 ex

 

The invention relates to medicine, namely to Pediatrics and Allergy, and can be used for early prediction of atopic diseases in children.

A known method for predicting the development of atopic dermatitis in infants by definitions in the cord blood of newborns absolute number of mononuclear cells that synthesize interferon-gamma (INFγand without activation marker CD69 (phenotype cells - INFγ+ CD69-). When the value of this index is 5.3 cells/ml and below, predict the development of atopic dermatitis in infants [EN 2330291 C1, publ. 27.07.2008]. The method allows to determine the risk of development of atopic allergic diseases in infants, but is costly and time-consuming to conduct, as it requires the isolation and cultivation of cells.

Closest to the claimed method is a method of forecasting the development of atopic allergic diseases in the newborn, based on the determination of the concentration of total IgE in serum of umbilical cord or peripheral blood of a newborn using enzyme immunoassay [EN 2108749 C1, publ. 20.04.1998]. The known method is insufficiently informative, as it does not reflect the cellular mechanism propensity to atopy. Moreover, the prognostic securities shall be such laboratory criteria as the level of serum IgE ambiguous.

The task of the invention to provide an informative method for predicting the development of atopic diseases in infants.

The technical result is obtained when implementing the claimed invention:

- expanding Arsenal of diagnostic tools providing the ability to predict the development of atopic diseases in infants;

early prediction of the development of atopic diseases in children;

- information content, reliability and safety of the method.

The essence of the claimed method is that in the plasma of umbilical cord blood of a newborn enzyme-linked immunosorbent assay to determine the level of interleukin-18 (IL-18), and when the level is equal to 34 PG/ml or lower predict the development of atopic disease in the newborn.

Interleukin-18 (IL-18) is a regulatory cytokine, similar to the cytokine family, IL-1 (on the structure, nature of the reception, the type of signal, anti-inflammatory properties), as well as with IL-12 (as inducer of Th1 cytokines profile and as a stimulator of T-cell cytotoxicity). Stimulates production of interferon-gamma by Th1 cells type, activates the EC and monocytes, has a strong influence on the processes of proliferation and cytotoxicity of T-lymphocytes and EC compared with IL-12 [Found on the Internet at: http://allimmunology.org/

immunologicheskij-slovr/i/interlejkin-18-19-20-i-21-il-18 il-19 il-20 il-21]. It is known that IL-18 is a pleiotropic, constitutively expressed cytokine-containing substances that occur in many cell types, mainly by activated monocytes and dendritic cells. The main effects of IL-18 is directed to stimulation of the production of such cytokines as γ, α, IL-1β, as well as adhesion molecules and factors that stimulate apoptosis. Inhibits the activity of cells of the Th2 type. IL-18 is most actively involved in anti-infective and anti-tumor defense of the organism. Shown the protective role of IL-18 in the mechanisms of development of atopic diseases, which led to the choice of this index as a marker of risk for development of atopic diseases in children [Peter J. Barnes. 2008. The cytokine network in asthma and chronic obstructive pulmonary disease. The Journal of Clinical Investigation. 118: 3546-3556. Found on the Internet at: http://www.jci.org/articles/view/36130].

Threshold value (34 PG/ml) was obtained empirically on the basis of a comparative study of research data on the content of interleukin-18 in umbilical cord blood of newborns. Studies have shown that the level of IL-18 is equal to 34 PG/ml (border 25 quartile in the sample authors) or lower risk for development of atopic disease in the newborn should be considered high.

The method is as follows. At birth produce fence 1-2 ml of umbilical cord blood, which is the center of fugeret, then select the plasma in the amount of 0.5 ml of the prepared plasma determine the level of IL-18 enzyme-linked immunosorbent assay using commercially available reagents, for example, companies Bender Medsystems. When the level of IL-18 is equal to 34 PG/ml or lower predict the development of atopic allergic diseases in the newborn. These children belong to the risk group for the development of atopic diseases.

Under the proposed method surveyed 57 infants. 20 children identified low levels of IL-18 (≤34 PG/ml), which gave rise to attribute them to the risk of development of atopic diseases. At the same time the mothers of these newborns in history registered simultaneously atopic dermatitis and clinical manifestations of allergic rhinitis (mother with the presence of atopic phenotype).

Example 1. Newborn female. Studied umbilical cord blood on the proposed method. ELISA determined the level of IL-18 of 22.44 PG/ml (below 34 PG/ml). Newborn baby, according to the invention, is classified as high risk for development of atopic diseases.

Example 2. A newborn male. Studied umbilical cord blood on the proposed method. ELISA determined the level of IL-18 - 47,22 PG/ml (above 34 PG/ml). Newborn baby, according to the invention, is classified as low risk developed by the Yu atopic diseases.

The use of the invention allows to determine the risk of development of atopic allergic diseases in infants and conduct early preventive measures for their prevention.

A method for predicting the development of atopic diseases in infants by study of umbilical cord blood enzyme-linked immunosorbent assay, characterized in that in the plasma of umbilical cord blood of a newborn determine the level of interleukin-18 and when the level is equal to 34 PG/ml or below, predict the development of atopic disease in the newborn.



 

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