Method of control of photoautotrophic microorganism cultivation process

FIELD: medicine.

SUBSTANCE: initial nutrient medium together with an inoculated autotrophic microorganism is supplied from a technological container into an input section of a photobioreactor with forming a suspension film of a photoautotrophic microorganism flowing down by gravity on an internal surface of transparent cylindrical tubes. Simultaneously, mixed air and carbon dioxide are reverse-flow supplied inside the tubes with using sleeves with suspension film outflow. The photoautotrophic microorganism suspension flowing in the internal surface of the transparent cylindrical tubes gets into a light section wherein it is continuously illuminated with a fluorescent tube. From the transparent cylindrical tubes, the photoautotrophic microorganism suspension flows down in an output section of the photobioreactor wherein it is bubbled to saturate the cells with carbon dioxide additionally and illuminated with a horizontal toroidal lamp. An external surface of the transparent cylindrical tubes is sequentially cooled in cooling air in the light section and in cooling water in the cooling section with cooling air and cooling water flowing in the respective recirculation loops. The photoautotrophic microorganism suspension is added with a nutrient medium of main and correction flows supplied into a technological container at first and then into the suspension recirculation loop at the input of the input section of the photobioreactor. Waste mixed air and carbon dioxide are supplied from the photobioreactor into the mixer by means of a compressor through the mixed air and carbon dioxide recirculation loop and temporarily collected in a gas tank. Post-bubble foam is continuously discharged from a lower section of the photobioreactor into an anti-foaming separator and separated into a suspension supplied into the input section of the photobioreactor and mixed air and carbon dioxide combined with waste mixed air and carbon dioxide in a regulation loop, while being temporarily collected in the gas tank and supplied into the mixer with extra saturation of waste mixed air and carbon dioxide with a required amount of carbon dioxide. Carbon dioxide saturated mixed air and carbon dioxide are discharged from the mixed by two ducts one of which being a main flow is reverse-flow directed inside the transparent cylindrical tubes; the other one is supplied into the output portion of the photobioreactor when bubbling the suspension. From the output portion of the photobioreactor, the microorganism suspension is discharged from the suspension recirculation loop with intermediate vented out oxygen release accompanying a cultivation process with using a desorber; another portion of the photoautotrophic microorganism suspension is discharged in a finished biomass collector to be measured for the required values for the purpose of creating optimal conditions for photoautotrophic microorganism cultivation.

EFFECT: invention provides higher effectiveness of photoautotrophic microorganism cultivation, enabled integration of the presented method into the current production lines, improved energy efficiency and performance of photoautotrophic microorganism cultivation.

2 ex, 1 dwg

 

The invention relates to the automation of microbiological processes of production and can be used to automate the process of cultivation photoautotrophic microorganisms.

The closest in technical essence and the achieved effect is the method of cultivation of photosynthetic microorganisms [RF Patent №2019564, IPC5C12Q 3/00. The method of cultivation of photosynthetic microorganisms and installation for its implementation / Volkovbot. No. 2005112085; Appl. 01.11.1990; publ. 15.09.1994. Bull. No. 26], including the regulation of the temperature of the suspension photoautotrophic microorganisms, the flow of suspension in the photoreactor, maintaining the maximum value of the intensity of photosynthesis by changing the temperature of the suspension and the alignment of photosynthesis with a controlled heat transfer, enrichment suspension of carbon dioxide in gazoobmenna, lighting photobioreactor artificial light source, cooling the suspension in the process of cultivation of cold coolant, remove emitted cultivation of oxygen with the help of dilution air, the measurement of oxygen concentration in suspension in the air, the temperature of the suspension, the regulation of the flow rate of cold fluid.

However, the known method has several disadvantages:

- does not allow to make full use of the camera is the implementation of the pH of the suspension during cultivation due to lack of operational flow of the nutrient medium and to create optimal conditions for biochemical reactions ensuring the stability of the growth of cells of microorganisms;

- does not provide for the discharge of the foam generated in the input gas mixture in suspension, making it difficult to move the slurry through pipelines and reduces the efficiency of the energy absorption of the light;

- when blowing the air suspension possible inhibition of photosynthesis by oxygen due to its incomplete removal;

- does not allow to fully increase the growth rate of the cells during cultivation photoautotroph microorganism, so as not supporting a certain ratio between the number of molecules of CO2and the number of quanta of light in accordance with the reaction of photosynthesis due to simultaneous changes of illumination from the light source and the concentration of carbon dioxide in the gas mixture;

- does not allow to reduce the field of tolerance on the temperature of the suspension and narrow it down variation in the cultivation process, which negatively affects the sustainability of biomass accumulation and the intensity of the process of biosynthesis for mesophilic and cryophilic photoautotrophic microorganisms;

- does not allow to reduce energy consumption per unit produced biomass due to the lack of accuracy and reliability of control of technological parameters during cultivation photoautotroph microorganism.

Technical is some object of the invention is to increase the efficiency of cultivation photoautotrophic microorganisms; intensification of synthesis of biologically active substances; creating opportunities for installation of the proposed method in existing production lines with alkalization manufactured products; reduce energy costs cultivation process photoautotrophic of microorganisms for the accuracy and reliability of control of technological parameters.

To solve the technical problem of the invention in the process control method of cultivation photoautotrophic microorganisms, which feed suspension photoautotroph microorganism in photobioreactor, enrichment suspension carbon dioxide, lighting photobioreactor artificial light source, cooling the suspension in the process of cultivation of cold coolant, remove emitted from the cultivation of the oxygen concentration measurement dissolved in a suspension of oxygen and the temperature of the suspension, the regulation of the flow rate of cold fluid, it is new that the cultivation photoautotrophic microorganisms is carried out in the film of the suspension flowing along the inner surface of the cylindrical transparent tube installed in photobioreactors, while the outer surface of the tube is cooled successively cooling air and cooling water, which cold talonite is s; in the suspension photoautotroph microorganism at the entrance to photobioreactor continuously introducing nutrient medium, the preparation of which exercise of its constituent primary and corrective streams feed first in technological capacity, and then in the recirculation circuit of the suspension at the entrance to photobioreactor; as an artificial light source using two fluorescent lamps; receive a mixture of air with carbon dioxide in the mixer and remove it from the mixer in two streams, one of which is directed inward of the cylindrical transparent tube in counter-current mode with a flowing film of suspension photoautotroph microorganism for the implementation of the carbon dioxide absorption film of the suspension, and the other on the bubbling suspension, continuously filling the lower part of photobioreactor; an elaborate mixture of air with carbon dioxide from photobioreactor first output in the gas tank, and then return to the mixer in closed loop mode with additional feeding carbon dioxide; foam arising from the bubbling continuously removed from the bottom of photobioreactor in the separator-antifoam, where it is divided into the suspension returned to photobioreactor, and the mixture of air with carbon dioxide, which combines with the proven mixture of air with carbon dioxide in the path of its recircula the AI before the mixer; after photobioreactor of suspension photoautotroph microorganism allocate formed during cultivation of oxygen in desorber with the subsequent return of one part of the suspension photobioreactor and output another part in the form of prepared biomass in the collection of harvest; additionally measure the optical density of the suspension photoautotroph microorganism outlet photobioreactor, the flow rate of the suspension photoautotroph microorganism in the recirculation circuit at the entrance to photobioreactor and in the feed line in the collection of the harvest; the flow of foam from the bottom of photobioreactor in the separator-antifoam; the pH of the suspension photoautotroph microorganism in the path of its recycling; the costs of the primary and corrective flow of the nutrient medium supplied to the recirculation circuit suspension the entrance to photobioreactor; costs of carbon dioxide and a mixture of air and carbon dioxide, guided in a cylindrical transparent tube and bubbling; the costs of cooling air and cooling water; the suspension level in the lower part of photobioreactor; and provide stabilization of the optical density of the suspension photoautotroph microorganism outlet photobioreactor in the range of specified values; and when the deviation of the optical density of the suspension photoautotroph microorganism from the interval specified value is in the lower side first synchronous increase the illumination of the cylindrical transparent tube, and the concentration of carbon dioxide mixed with air before reaching their maximum values, then increase the consumption of nutrient medium inlet photobioreactor before reaching its maximum value and then affect the cost of suspension photoautotroph microorganism in the recirculation circuit of the suspension and the drain line from the recirculation circuit in the collection of the crop by increasing the flow rate of the suspension in the recirculation circuit, while reducing the performance photobioreactor finish biomass, and when the deviation of the optical density of the suspension on an interval of the preset values in a big way at first affect the cost of suspension photoautotroph microorganism in the recirculation circuit of the suspension and the drain line from the recirculation circuit in the collection of the crop by increasing the flow rate of the suspension in the collection of the harvest, increasing the performance photobioreactor finish biomass, then reduce the consumption of the nutrient medium and the light to the output end of the optical density of the suspension at the upper limit of the specified range of values; the preparation of the nutrient medium are carried out by a given value of pH of the suspension to the contour of her recirculation effects on the ratio of costs of primary and corrective flow of the nutrient medium; establish a flow mixture of air and carbon dioxide in the flow of the suspension at the entrance to photobioreactor impact on consumption, ug is ecologo gas, supplied through the line feeding into the mixer, with correction for the concentration of carbon dioxide in a mixture of air and carbon dioxide at the outlet of photobioreactor; when exceeding the maximum rated pressure of the mixture of air with carbon dioxide in the gas tank and the mixer produces a discharge pressure of the mixture of air and carbon dioxide through the safety valve; the temperature of the suspension photoautotroph microorganism in photobioreactor regulate the cost of cooling air and cooling water for cooling the outer surface of the tubes; the current value of the concentration dissolved in the suspension of oxygen in the path of its recycling affect the flow of oxygen in the line of withdrawal of desorber; flow of foam from the bottom of photobioreactor set the drive power of the separator-antifoam.

The technical result of the invention is to improve the efficiency of cultivation due to the intensification of biomass growth and synthesis of biologically active substances by improving the accuracy and reliability of control of technological parameters, as well as by increasing the number of potential control actions on the process of cultivation photoautotrophic microorganisms; in addition, the technical result consists in creation of conditions for strayan the I of the proposed method in existing production lines with alkalization manufactured products, as well as to improve energy efficiency by reducing energy consumption.

Figure 1 presents a scheme that implements the proposed method of control the culturing process photoautotrophic microorganisms.

The schema contains photobioreactor 1, consisting of the input section 2, section lighting 3, cooling 4 and output section 5, containing fluorescent lamps: 6 cylindrical and toroidal 7, a transparent cylindrical tube 8, the sockets for input into the tubes 8 a mixture of air and carbon dioxide 9 and bubbler 10; the collection of harvest 11 and technological capacity 12; ultrademocratic for the regeneration of the cooling air 13 and the cooling water 14; mixer air with carbon dioxide 15; gas tank 16; desorber oxygen 17; separator-antifoam 18; a circulating pump 19, the pump 20, the compressor 21, a fan 22 and 23; the collector 24; distributors threads 25 and 26; the microprocessor 27; contours recycling: suspension photoautotroph microorganism 0.1.1, a mixture of air and carbon dioxide 5.7, cooling air 3.2, cooling water 1.1; line feed: ready biomass in the collection of harvest 0.1.2, main 0.2.1 and corrective 0.2.2 flow of the nutrient medium, carbon dioxide 5.4, a mixture of air and carbon dioxide in a transparent cylindrical tube 5.7.1 and bubbler 5.7.2; line challenge: finish biomass from the collection of yield is 0.1.3, foam from the output section of photobioreactor 0.3, suspension from the separator-antifoam 0.1.4, a mixture of air and carbon dioxide from the separator-antifoam 5.7.3, oxygen 3.7, a mixture of air and carbon dioxide from the mixer 5.7.4 and from the gas tank 5.7.5; sensors: TE - temperature FE - flow, LE - level, pH, QE - optical density, CE - concentration; And enforcement mechanisms; ↓ input channels of control; ↑ - output control channels.

The process control method of cultivation photoautotrophic microorganisms is carried out as follows.

Source nutrient medium photoautotroph microorganism served from the process vessel 12 in the input section 2 film photobioreactor 1, where the inner surface of the transparent cylindrical tube 8 is formed pellicle suspension photoautotroph microorganism, which gravity flows down. At the same time inside a transparent cylindrical tube 8 through pipe 9 serves a mixture of air with carbon dioxide in countercurrent mode with expired film suspension. In the flow along the inner surface of the transparent cylindrical tube 8 suspension photoautotroph microorganism enters the lighting section 3, in which it continuously illuminate a cylindrical fluorescent lamp 6. As a result of joint effects on suspen the July mixture of air and carbon dioxide and light in the process of cultivation, characterized by the absorption of carbon dioxide from its mixtures with air (gas phase) film suspension. Absorption facilitates the movement of molecules of CO2cells photoautotroph microorganism and their assimilation. When this occurs the photosynthesis reaction, accompanied by the accumulation of organic matter in the cells, their growth and development.

Of the transparent cylindrical tube 8 suspension photoautotroph microorganism flows in the output section 5 photobioreactor where it is bubbling a mixture of air and carbon dioxide through the bubbler 10 and illuminated horizontal toroidal fluorescent lamp 7. The presence of the bubbler at the bottom of photobioreactor also allows us to saturate cells photoautotroph microorganism carbon dioxide, together with horizontal lighting lamp gives you the ability to perform photosynthesis cells and in the output section, and to prevent sedimentation of the cells on the inner walls of the output section of photobioreactor when using strains photoautotrophic microorganisms that do not have planktonic properties.

In the result of joint work of fluorescent lamps 6 and 7 generates heat, which should be given to ensure the necessary conditions of the cultivation process photoautotroph microorganism and high quality the STV prepared biomass. Therefore, the method provides consistent cooling of the outer surface of the transparent cylindrical tube 8 cold fluids - cooling air in the lighting section 3 and the cooling water in the cooling section 4. Thus the cooling air and cooling water are moved along the contours recirculation respectively 3.2 and 1.1 with the help of the fan 22 and pump 20 with regeneration in ultrademocratic 13 and 14.

An elaborate mixture of air with carbon dioxide from photobioreactor fed into the mixer 15 with the aid of the compressor 21 in the recirculation circuit mixture of air and carbon dioxide 5.7 intermediate in the gas collection tank 16. The foam arising from the bubbling of suspension in section 5 photobioreactor, continuously divert line 0.3 separator-antifoam 18. In the separator-defoamer foam divided into suspension, returned in section 2 photobioreactor line 0.1.4, and the mixture of air with carbon dioxide, which is in line 5.7.3 sent to the recirculation circuit mixture of air and carbon dioxide 5.7 and thereby combine with the proven mixture of air with carbon dioxide, leaving photobioreactor in the mixer 15. In the mixer additionally carry out the feeding of the waste mixture of air and carbon dioxide on line 5.4 the amount of carbon dioxide equal used for cultivation photoaptamer mnogo microorganism in photobioreactor.

From the mixer 15 the mixture of air with carbon dioxide using a dispenser threads 25 are divided into two streams, one of which is sent as a main flow line 5.7.1 in the manifold 24 with typing inside a cylindrical transparent tube, and the other on the line 5.7.2 in the bubbler 10. The collector 24 is structurally designed in such a way that allows you to divide the main stream into equal parts, the number of which corresponds to the number of transparent cylindrical tube 8.

From section 5 photobioreactor suspension photoautotroph microorganism display in the recirculation circuit suspension photoautotroph microorganism 0.1.1 circulating pump 19 with the release of the suspension in desorber 17 formed in the process of cultivation of oxygen and its outlet line 3.7 by fan 23.

One part of the suspension returns to photobioreactor the recirculation circuit suspension photoautotroph microorganism 0.1.1, and the other as a ready-to divert biomass in the collection of harvest 11. The selection of final biomass in the collection of harvest on line 0.1.2 carried out using the flow distributor 26, from line 0.1.3 she served in the main production (in the case of embedding the proposed method into an existing production line by alkalization manufactured products) or released as g the postal products to the consumer.

Information about the progress of the cultivation process photoautotrophic microorganisms using sensors is transmitted to the microprocessor 27, which is embedded programmable logic algorithm performs operational control of process parameters by means of the actuators with the accounting overlayed limitations as intensive obtaining biomass and economic feasibility.

The microprocessor 27 is continuously compares the current value of the optical density of the suspension photoautotroph microorganism with an interval of the preset values. When the deviation of the optical density of the suspension photoautotroph microorganism on an interval of the preset values in the lower side of the microprocessor 27 first synchronous increases the illumination of the cylindrical transparent tube 8 lamps 6, 7 and the concentration of carbon dioxide in a mixture with air by increasing its flow into the mixer 15 through the line 5.4 to achieve their maximum values.

A synchronous increase in the illumination of the cylindrical transparent tube, and the concentration of carbon dioxide due to the necessary condition in the process of cultivation photoautotrophic microorganisms, based on the reaction of photosynthesis [Mokrousov A.T. and other Photosynthesis. Physiological-ecological and bio is himicheskie aspects. - 2006. - 448].

If a synchronous increase in the illumination of the cylindrical transparent tube, and the concentration of carbon dioxide does not allow to display the current value of the optical density photoautotrophic microorganism on the interval specified values, then the microprocessor increases the consumption of the nutrient medium at the entrance to photobioreactor 1 until reaching its maximum value.

If the result of comparison of the current value of the optical density of the suspension will not reach the interval specified values, then the microprocessor affects the cost of suspension photoautotroph microorganism in the recirculation circuit suspension 0.1.1 and line removal 0.1.2 from the recirculation circuit in the collection of harvest 11 by increasing the flow rate of the suspension in the recirculation circuit, while reducing the performance photobioreactor finish biomass.

When the deviation of the optical density of the suspension photoautotroph microorganism on an interval of the preset values in a big way, the microprocessor 27 the first is the effect on the ratio of costs of suspension photoautotroph microorganism in the recirculation circuit suspension 0.1.1 and the drain line from the recirculation circuit in the collection of harvest 0.1.2 by increasing the flow rate of the suspension in the collection of the harvest, increasing the performance photobioreactor finish biomass satisnet consumption of the nutrient medium in the lines 0.2.1 and 0.2.2, the light and the concentration of carbon dioxide in a mixture with air to exit the optical density of the suspension photoautotroph microorganism on the upper limit of the specified range of values.

The ratio of costs the main and correction of nutrient media, respectively, in lines 0.2.1 and 0.2.2 set to the current value of the pH of the suspension photoautotroph microorganism in the recirculation circuit 0.1.1. Optimal for most photoautotrophic microorganisms is neutral, alkaline or slightly alkaline environment. In the process of their cultivation, as a rule, there is a "alkalization" suspension, so the main nutrient medium should have an alkaline reaction, and correcting acid. As a corrective environment using ammonium nitrate NH4NO3.

The microprocessor 27 to the current flow of the main flow of the nutrient medium in line 0.2.1 sets the flow of the mixture of air with carbon dioxide in the contour of her recirculation 5.7.

On the current flow of the suspension photoautotroph microorganism in the recirculation circuit 0.1.1 microprocessor 27 sets the flow rate of the waste gas mixture in the mixer 15 through the recirculation circuit 5.7 impact on the adjustable drive of the compressor 21 with the correction current expenses of the gas mixture in the supply lines to the bubbling 5.7.2 and cyl is Harikesa transparent tube 5.7.1.

The microprocessor sets the flow rate of carbon dioxide in the mixer 15 through the line 5.4 on the current flow of waste gas mixture in the recirculation circuit 5.7 correction on the current concentration in the mixture of carbon dioxide.

When exceeding the maximum rated pressure of the mixture of air with carbon dioxide in the gas tank 16 and the mixer 15 is an emergency pressure relief mixture of air and carbon dioxide through the pressure relief valves respectively on the lines 5.7.5 and 5.7.4. The gas capacity of 16 due to the need to ensure stable and smooth collaboration of all elements of the scheme, in case of disturbing internal and external influences.

The temperature of the suspension photoautotroph microorganism in photobioreactor the microprocessor adjusts the cost of the cooling air and cooling water, respectively, in the circuits of the recirculation 3.2 and 1.1 impact drives the fan 22 and pump 20.

The use of cooling air and cooling water as cold fluids in various sections of photobioreactor due to its design features, in particular the presence in the section of the lighting of a fluorescent lamp does not allow the cylindrical tube cooled with water.

According to the current value of the concentration of alvarenga suspension of oxygen in the recirculation circuit 0.1.1 microprocessor sets the power adjustable fan drive 23 in line 3.7 removal of gaseous oxygen from desorber 17.

On the current flow of foam in line 0.3 microprocessor sets the required power adjustable drive cage-antifoam 18.

In case of exceeding the upper limit is the level of suspension photoautotroph microorganism in section 5 photobioreactor the microprocessor disables the flow of the nutrient medium from the process vessel 12 in the recirculation circuit 0.1.1, and when you reach the bottom of the set value of the level of suspension in section 5 performs a corresponding increase of consumption of the nutrient medium from the tank 12 into the recirculation circuit 0.1.1.

Examples of implementation of the method.

The process control method of cultivation photoautotrophic microorganisms implemented in pilot conditions on the territory of Voronezh experimental feed mill.

The cultivation process photoautotrophic microorganisms was carried out according to the above method using a film photobioreactor [RF Patent №2363728, IPC7C12M 1/04, C12M 1/06, 3/28 B01D. Film camera / AIO, Essencia, Avjennings, Avemore No. 2008118450; Appl. 13.05.2008; publ. 10.08.2009, Bulletin No. 22] with the following technical characteristics:

the temperature of cultivation20...50°C

op the practical density of the suspension photoautotroph microorganism:

the initial suspension0,2...0,4% opt. square
willing suspension1,5...2,0% opt. square
the performance of the finished suspension400...600 DM3/d

the flow of the mixture of air with carbon dioxide:

in a transparent cylindrical tube0.30 m3/(h·l)
the sparging0,07 m3/(h·l)
the concentration of carbon dioxide in a mixture with air3...8%
the initial illumination10...50 KLX
the flow of the nutrient medium5 DM3/h
the initial flow of the cooling air1,0...1,5 m3/h
the initial flow rate of cooling waterof 0.05...0.15 m3/h

housing dimensions:

height3.2 m
diameter1.1 m

characteristics of cylindrical transparent tube:

height2.5 m
diameter0,07 m

The cultivation process photoautotrophic microorganisms was dosed supply of the primary and corrective flow of the nutrient medium, a mixture of air and carbon dioxide, cooling air and cooling water to compensate for thermal discharge from the lamps. From the recirculation circuit suspension photoautotroph microorganism took away the oxygen produced during photosynthesis cells. When the suspension photoautotroph microorganism given value of optical density due to the increase in the number of cells of the microorganism suspension part of it - ready suspension was taken in the compilation of yield with simultaneous supply is equivalent to the volume of the nutrient medium.

The temperature stability of the suspension photoautotroph microorganism during cultivation was carried out as follows. The only factor that caused the destabilization of the temperature, was heated suspension photoautotroph microorganism loom is ascentii lamps, therefore, the deviation of the current value of the temperature of the suspension, as a rule, occurs only in a big way on an interval of the preset values. For cooling was used for cooling air and cooling water with a temperature of 10°C. it is Known that fluctuations in the temperature of the suspension within 5°With a positive impact on the intensity of biomass growth [Konstantinov, A.S., etc. // Vestnik Mosk. University (ser. biology). - 1998. No. 1. P.47 - 50].

Example No. 1. As the object of cultivation used microscopic green Alga Chlorella vulgaris (strain UA-1-20).

In the protein Chlorella vulgaris contains all essential amino acids, various trace elements, natural antibiotic lorelin, arachidonic acid (conditionally essential, normalizes reproductive function), hlon "A" (induces interferon production). For cells of Chlorella vulgaris is the norm selection in suspension of various useful products of metabolism (ekzometabolitov) [Stanchev P. // Hydrobiology. - 1980. No. 10. - P.70-77.].

As a nutrient medium for cultivation of Chlorella vulgaris was used environment Tamiya (modification Kuznetsova and the Vladimirova)having the following composition, g/l:

1. KNO35,0
2. MgSO4·7H2O 2,5
3. KH2PO4·3H2O1,25
4. Na2EDTA0,037
5. FeSO4·7H2O0,009

6. Trace elements 1 ml

The composition of trace element solution, g/l:

6.1. H3BO32,860
6.2. MnCl2·4H2About1,810
6.3. ZnSO4·7H2O0,222
6.4. MoO30,015
6.5. NH4VO30,023

Cultivation of green algae Chlorella vulgaris was carried out on the proposed control method with the following process parameters:

given the range of values of temperature cultivation20...37°C

the optical density of the suspension:

the initial suspension0,4% of the protected area. square
willing suspension1,7 1,5...unit opt. square
the initial illumination10...15 klk
the initial flow of the cooling airthe 1.3...1.5 m3/h
the initial flow rate of cooling water...0,15 0,10 m3/h

The output of suspension of microscopic algae Chlorella vulgaris (strain UA-1-20) on the proposed method amounted to 470 510...DM3/day at a concentration of absolutely dry matter 3,5...3.7 g/l

Example No. 2. As the object of cultivation used cyanobacterium Spirulina platensis (Nordst.) Geitl. (spirulina).

The biomass of Spirulina platensis derived from its suspension, is used as a food additive in the diet of humans and animals. Cells of Spirulina platensis is a rich source of micronutrients (iodine, selenium etc). Spirulina is used in medicine, cosmetics, livestock, poultry, etc. From biomass of Spirulina platensis get amino acids, protein, carbohydrates, lipids, pigments, vitamins, etc. [Berestov, VA Spirulina: yesterday, today, tomorrow. - S.-Pb.: DOE, 2002].

As a nutrient medium for cultivation of Spirulina platensis was used environment Zaruka having the following composition, g/l:

1. NaHCO316,8
2. K2HPO4·3H2O0,66
3. NaNO32,5
4. K2SO4·4H2O1,0
5. NaCl1,0
6. Na2EDTA0,08
7. FeSO4·7H2O0,01
8. CaCl4·2H2O0,04
9. MgSO4·7H2O0,2
10. Trace element solution 1 ml

The solutions of trace elements, g/l:

tr>
Solution 1.Solution 2.
10.1. H3BO32,86010.6. With NISO4·7H2O0,045
10.2. MnCl2·4H2O1,81010.7. NH4VO30,023
10.3. ZnSO4·7H2O0,22210.8. Na2WO4·2H2O0,018
10.4. CuSO4·5H2O0,08010.9. CoCl2·6H2O0,044
10.5. MoO30,01510.10. K2Cr2(SO4)2·24H2O0,096

Cultivation of cyanobacteria Spirulina platensis (Nordst.) Geitl was held on the proposed control method with the following process parameters:

the temperature of cultivation:

given the range of values of temperature cultivation29...43°C

the optical density of the suspension:

the initial suspension0,2% of the protected area. square
willing suspension1,9 2,1...unit opt. square
the initial illumination25...30 club
the initial flow of cooling air, the 1,0...1.1 m3/h
the initial flow rate of cooling waterof 0.05...0.10 m3/h

The output of suspension cyanobacterium Spirulina platensis on the proposed method was 400...415 DM3/day at a concentration of 4.0...4.3 g ASV/L.

Thus, the proposed method of control the culturing process photoautotrophic microorganisms allows you to:

- to create optimal conditions for growth of the microorganism cells by stabilizing the pH of the suspension during cultivation;

to improve the movement of the slurry through pipelines and to improve the efficiency of energy absorption of the light due to the removal of the foam generated in the input gas mixture in suspension;

- to increase the growth rate of the cells during cultivation photoautotroph microorganism due to simultaneous changes of illumination from the light source and the concentration of carbon dioxide in the gas mixture;

- to ensure the sustainability of biomass accumulation and the intensity of the process of biosynthesis for mesophilic and cryophilic photoautotrophic microorganisms by narrowing the field of tolerance on the temperature of the suspension and reduce its spread in cultivation;

- reduce specific energy consumption by 10...12% due to increased accuracy and reliability and control of technological parameters during cultivation photoautotroph microorganism;

to embed the proposed technology in the composition of the existing production lines.

The process control method of cultivation photoautotrophic microorganisms, which feed suspension photoautotroph microorganism in photobioreactor, enrichment suspension carbon dioxide, lighting photobioreactor artificial light source, cooling the suspension in the process of cultivation of cold coolant, remove emitted from the cultivation of the oxygen concentration measurement dissolved in a suspension of oxygen and the temperature of the suspension, the regulation of the flow rate of cold fluid, characterized in that the cultivation photoautotrophic microorganisms is carried out in the film of the suspension flowing along the inner surface of the cylindrical transparent tube installed in photobioreactors, while the outer surface of the tube is cooled successively cooling air and cooling water, which is cold fluids, suspension photoautotroph microorganism at the entrance to photobioreactor continuously introducing nutrient medium, the preparation of which exercise of its constituent primary and corrective streams feed first in technological capacity, and then in the recirculation circuit of the suspension at the entrance to photobioreactor, to the operation of the artificial light source using two fluorescent lamps, receive a mixture of air with carbon dioxide in the mixer and remove it from the mixer in two streams, one of which is directed inward of the cylindrical transparent tube in counter-current mode with a flowing film of suspension photoautotroph microorganism for the implementation of the carbon dioxide absorption film of the suspension, and the other on the bubbling suspension, continuously filling the lower part of photobioreactor, an elaborate mixture of air with carbon dioxide from photobioreactor first output in the gas tank, and then return to the mixer in closed loop mode with additional feeding carbon dioxide, foam arising from the bubbling continuously removed from the bottom of photobioreactor in the separator-antifoam where it is divided into the suspension returned to photobioreactor, and the mixture of air with carbon dioxide, which combines with the proven mixture of air with carbon dioxide in the contour of her recirculation mixer before, after photobioreactor of suspension photoautotroph microorganism allocate formed during cultivation of oxygen in desorber with the subsequent return of one part of the suspension photobioreactor, and output another part in the form of prepared biomass in the collection of the harvest, additionally measure the optical density of the suspension photoautotroph microorganism to output the e from photobioreactor, the flow of suspension photoautotroph microorganism in the recirculation circuit at the entrance to photobioreactor and in the feed line in the collection of the harvest, the flow of foam from the bottom of photobioreactor in the separator-defoamer, pH of the suspension photoautotroph microorganism in its path recycling, the cost of primary and corrective flow of the nutrient medium supplied to the recirculation circuit of the suspension at the outlet in photobioreactor, the costs of carbon dioxide and a mixture of air and carbon dioxide, guided in a cylindrical transparent tube and bubbling, the cost of cooling air and cooling water, the level of suspension in the lower part of photobioreactor, and provide stabilization of the optical density of the suspension photoautotroph microorganism output from photobioreactor in the range of specified values, and the deviation of the optical density of the suspension photoautotroph microorganism on an interval of the preset values in the lower side first synchronous increase the illumination of the cylindrical transparent tube, and the concentration of carbon dioxide mixed with air before reaching their maximum values, then increase the flow rate of the nutrient medium at the entrance to photobioreactor before reaching its maximum value, and then affect the cost of suspension photoautotroph of the microorganism conture recirculation of the suspension and the drain line from the recirculation circuit in the collection of the crop by increasing the flow rate of the suspension in the recirculation circuit, while reducing the performance photobioreactor finish biomass, and when the deviation of the optical density of the suspension on an interval of the preset values in a big way at first affect the cost of suspension photoautotroph microorganism in the recirculation circuit of the suspension and the drain line from the recirculation circuit in the collection of the crop by increasing the flow rate of the suspension in the collection of the harvest, increasing the performance photobioreactor ready for biomass, then reduce the consumption of the nutrient medium and the light to the output end of the optical density of the suspension at the upper boundary of the specified ranges, the preparation of the nutrient medium are carried out by a given value of pH of the suspension to the contour of her recirculation effects on the ratio of costs of primary and corrective threads medium, set the flow mixture of air and carbon dioxide in the flow of the suspension at the entrance to photobioreactor impact on consumption, carbon dioxide supplied through the line feeding into the mixer, with correction for the concentration of carbon dioxide in a mixture of air and carbon dioxide at the outlet of photobioreactor, when exceeding the maximum rated pressure of the mixture of air with carbon dioxide in the gas tank and the mixer produces a discharge pressure of the mixture of air and carbon dioxide through the pre is protective valves, the temperature of the suspension photoautotroph microorganism in photobioreactor regulate the cost of cooling air and cooling water for cooling the outer surface of the tube, the current value of the concentration dissolved in the suspension of oxygen in the path of its recycling affect the flow of oxygen in the line of withdrawal of desorber, the flow of foam from the bottom of photobioreactor set the drive power of the separator-antifoam.



 

Same patents:

FIELD: chemistry; biochemistry.

SUBSTANCE: proposed method can primarily be used in biotechnology, biochemistry and industrial microbiology. Fermentation apparatus are used to study growth and metabolism of microorganisms and for solving several other tasks. Proposed solution involves measurement within given time intervals of flow of liquid and gaseous media through a fermentation vessel at the beginning of the fermentation process and during the said process after selected time intervals necessary for measuring heat production of microorganisms and evaluating destabilising inputs of heat power from operation of apparatus for moving the culture fluid. Heat production is calculated as the increment of current values of heat power to the initial value of the measured heat power while making corrections for the effect of the said destabilising inputs. The method is realised in a fermentation apparatus in which a fermentation vessel is placed inside a controlled thermostating screen and is fitted with an additional mixing device for controlling temperature of the fermentation vessel. Pipes running to the fermentation vessel are in thermal contact with the controlled thermostating screen.

EFFECT: more accurate measurement of heat production of microorganisms in a fermentation vessel in continuous or periodic processes.

4 cl, 5 dwg, 1 tbl

FIELD: biotechnology and microbiological industry.

SUBSTANCE: invention concerns governing periodical air-intake biotechnological process carried out in bioreactor. Method comprises measuring oxygen content in effluent gas, working volume of culture medium, concentration of biomass, and concentration of intermediate product of its vital activity. Measured parameters allow specific oxygen consumption rate and velocity of intermediate product concentration change to be determined to enable regulation of feeding air used in aeration, supplying nutritional medium, and agitating culture medium. Moreover, temperature of culture medium, temperature of supplied and withdrawn cooling agent, and consumption of the latter are measured to use these parameters for determining biomass heat release rate and velocity of intermediate product amount change. The two latter parameters enable regulation of feeding air used in aeration and supplying nutritional medium. The following characteristics are thus improved: elevating power by 8.1%, maltase activity by 7.9% and resistance by 7.4%.

EFFECT: enhanced efficiency of governing biotechnological process and improved qualitative characteristics of process.

2 ex

The invention relates to the microbiological industry, and specifically to the production of Baker's yeast

The invention relates to pharmaceutical and biotechnological production, and can also be used in wastewater treatment, production using fermentation and fermentation

The invention relates to the microbiological industry, and in particular to methods of automatic control of the process of growing microorganisms

The invention relates to the microbiological industry, and can be used in agriculture to control the fermentation process of organic raw materials

The invention relates to the microbiological industry, and in particular to methods of automatic control of the process of growing microorganisms, and can be used in the production of bakery yeast
The invention relates to microbiological control and can be used in microelectronics, bio - and medical technologies for the control of bacteria in ultrapure water

FIELD: agriculture.

SUBSTANCE: water is taken, which contains algae. The algae mass 8 is centrifuged together with water at the speed of not more than 1000 rpm. Afterwards the algae weight 8 is exposed to a light diode device 5 including radiators of blue, green and red colours. Light radiators 5 are installed at the distance of not more than 50 cm from the surface of the centrifuged algae mass 8. The algae mass 8 is dried with dry warm air without access of direct sunlight. The system to produce the mass of blue-green algae from water reservoirs comprises a water-intake device, a centrifuge 1, light radiators 5, a heat gun or a heat fan 4. The water-intake device sends the algae mass 8 into the centrifuge 1. The centrifuge 1 is made as capable of centrifuging the mixture at the speeds of up to 1000 rpm. Inside or near the centrifuge 1 there are radiators 5 of blue, green and red colours. Light radiators 5 are installed at the distance of not more than 50 cm from the surface of the centrifuged algae mass 8. The heat gun or the heat fan 4 are installed as capable of blowing through the algae mass 8. The system comprises a cover 6 or is made in the form of a box restricting access of direct sunlight to the algae mass 8.

EFFECT: higher efficiency in production of dry blue-green algae mass from a water reservoir.

2 cl, 15 dwg, 4 tbl

FIELD: chemistry.

SUBSTANCE: when culturing phototrophs, the culture fluid is stirred and aerated through agitation by moving cultivators back and forth in the horizontal plane at given temperature and pH values. The cultivators are illuminated with a pulsed light source with pulse duration of 0.00001-0.001 s and pulse spacing of 0.01-0.1 s. In the apparatus used, the culture fluid is illuminated with diodes located under transparent bottoms of vessels of the same geometric shape and powered by a pulse generator with controlled frequency and light pulse duration.

EFFECT: group of inventions enables to reduce power consumption when culturing phototroph biomass.

2 cl, 2 dwg, 2 ex

FIELD: chemistry.

SUBSTANCE: disclosed is a Saccharomyces cerevisiae VKPM Y-3415 yeas strain which produces ethyl alcohol. The strain is adapted to culturing on dairy cheese whey.

EFFECT: strain is capable of producing ethyl alcohol with high output and is antagonistic to accompanying microflora.

1 ex

FIELD: chemistry.

SUBSTANCE: biosensor contains photo-autotrophic microalgae cells, the fluorescent characteristics of the photosynthesis system of which vary in the presence of cytotoxic chemical compounds in their surroundings: heavy metal ions and herbicides. Cells of green and diatomic microalgae are immobilised in cryogenic gel of polyvinyl alcohol: a cell suspension is deposited on a surface and the cells enter macropores of the polymer carrier under the effect of centrifugal force (5000-14000 g) for 1-10 minutes. A highly sensitive and stable biosensor is obtained based on components taken in the following ratio in wt %: microalgae cells 0.015-1.1; polyvinyl alcohol 7-15; aqueous phase - up to 100. Low concentrations of heavy metals and herbicides in aqueous systems are determined at flow rate of up to 360 ml/h based on the change in the value of relative variable fluorescence chlorophyll cells in the biosensor. The biosensor can be used for a maximum of 60 days.

EFFECT: high sensitivity of the biosensor.

2 dwg, 5 ex

FIELD: biotechnologies.

SUBSTANCE: method consists in extraction of lipid fraction from Chlorella microalgae biomass. Cell membranes of Chlorella microalgae are destroyed in device that develops vortex electromagnetic field with chaotically moving ferromagnetic particles that affect raw materials. Produced suspension of biomass is exposed to extraction with organic dissolvent by application of pulse-cavitation effect in rotor pulse-cavitation device.

EFFECT: production of lipid fraction from biomass of microalgae with higher yield and its further application as raw material for biodiesel fuel.

1 tbl, 3 ex

FIELD: medicine; biotechnologies.

SUBSTANCE: way includes cultivation of fresh-water diatoms Synedra Acus in a photobioreactor on the medium containing an isotope 13C-containing component. The seaweed selected from a natural source is used for inoculation, or a biomass preliminary enriched by an isotope 13C. Cultivation is performed in the conditions excluding access of extraneous sources of carbon from the environment. In quality of isotope-containing reagent bicarbonate NaH13CO3 is used. Further biomass selection, its filtering, homogenising and extracting of the common lipids using an organic dissolvent is performed. Hydrolysis of the common lipids and the subsequent clearing of a target product by chromatography is carriedout. The way allows raising enrichment level with a stable isotope of received polynonsaturated fatty acids. Degree of enrichment by an isotope 13C of palmitoleic acid makes 60%, stearin acid - 18%, linolenic acid - 75%, eicosapentanoic acid - 64%.

EFFECT: rising of degree of enrichment by an isotope.

6 cl, 2 dwg, 1 tbl, 2 ex

FIELD: chemistry; biochemistry.

SUBSTANCE: proposed method involves continuous culturing of an auxotrophic marine microorganism in a fermenter in aerobic conditions, in a culture medium with Y (output), g/l of dry cell substance, CDM. Y equals 100-300 g/l. The culture medium contains a carbon and nitrogen source, added gradually. The carbon source is used in quantity (Y x h) g/l of cultural broth, where h equals 1.1-3.0. The carbon source limits formation of biomass. The nitrogen source is used in quantity (Y x h x f) g/l of culture broth, where f equals 0.002-0.2. The time of stay in the fermenter is 20-100 hours. At least 40% of the obtained biomass consists of components, capable of being extracted using a chloroform and methanol mixture. Output of polyene acids is 0.2 g DHA/l/hr.

EFFECT: high output of polyene acids.

13 cl, 5 ex

FIELD: biotechnology, microbiology.

SUBSTANCE: invention proposes the strain Arthrospira platensis (Nordst.) Geitl. 1/02-T/03-5 isolated form the clone culture Arthrospira platensis 1/02. The strain is stored in collection of NIL VIE geographic faculty of Moscow State University. The strain is characterized by the enhanced yield of protein biomass wherein the mass part of protein is 65.6%.

EFFECT: increased content and yield of protein.

3 tbl, 3 dwg, 5 ex

FIELD: food-processing and microbiological industry, in particular, processes for extracting of biological preparations from cyanobacteria, may be used for enrichment of food products and producing of biologically active substances.

SUBSTANCE: method involves providing aqueous extraction and flotation precipitation using ammonium sulfate having saturation concentration of 20-70%; upon termination of each stage, providing centrifuging and separation of protein sediment; processing the latter on diethylaminoethyl activated carrier using ion-exchange chromatography method, followed by processing on membrane installation until saline content is below 5%; drying ready product to moisture content below 3%; using dry spirulina biomass enriched with selenium, chromium and other essential microelements. Method allows protein product with increased purity extent to be obtained, said product being characterized by containing phycocyanin and essential microelements and being used as source of these microelements and/or as food colorant which may be produced in commercial scale.

EFFECT: simplified method, reduced expenses for obtaining of ready product, and provision for producing of high-purity phycocyanin preparation.

5 cl, 2 dwg, 6 tbl, 3 ex

FIELD: biochemistry, in particular, methods and devices for producing coloring substances, possible use in food and cosmetic industry, and also during various biological research.

SUBSTANCE: phycoerythrin protein pigment is produced by extraction from seaweed. It is extracted from seaweed, selected from a group including Galaxaura oblongata, Halymenia ceylanica, Helminthocladia australis and Porphyra dentate.

EFFECT: phycoerythrin has high optical density.

2 cl, 27 dwg, 2 tbl

FIELD: chemistry.

SUBSTANCE: biosensor electrode is made from platinum on a polypyrrole base with a mediator. The mediator used is a pyrrole derivative containing ferrocene. The bioreceptor used is an enzyme which is immobilised on magnetic nanoparticles of Fe2O4 with surface carboxyl groups for cross-linking with carbomide, wherein the biosensor electrode with the bioreceptor is placed in a housing made from semiconductor material with possibility of connection to a digital-to-analogue converter.

EFFECT: invention enables to obtain compact devices with short response time and high detection sensitivity.

3 tbl

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