Method of culturing phototrophs and apparatus for realising said method

FIELD: chemistry.

SUBSTANCE: when culturing phototrophs, the culture fluid is stirred and aerated through agitation by moving cultivators back and forth in the horizontal plane at given temperature and pH values. The cultivators are illuminated with a pulsed light source with pulse duration of 0.00001-0.001 s and pulse spacing of 0.01-0.1 s. In the apparatus used, the culture fluid is illuminated with diodes located under transparent bottoms of vessels of the same geometric shape and powered by a pulse generator with controlled frequency and light pulse duration.

EFFECT: group of inventions enables to reduce power consumption when culturing phototroph biomass.

2 cl, 2 dwg, 2 ex

 

The invention relates to the field of biotechnology, in particular to methods of cultivation of phototrophs, and can be used for the cultivation of phototrophs to reduce energy consumption and simultaneous biological regeneration air of life support systems (WCS), in industrial, domestic and agricultural premises and obtaining biologically active substances (BAS) for the food, cosmetic, chemical industry and agriculture.

Cultivation of phototrophs with cost-effective lighting, with constantly rising energy prices and the deterioration of environmental conditions is an important task of the world level. One possible solution to the problem is the implementation of the method of cultivation of phototrophs using intermittent (pulsed) light.

There are various methods of cultivation of phototrophs. For example, the cultivation in open ponds (glodok L.P. Hardware technological design process of cultivation of cyanobacteria Spirulina. Dissertation, Moscow, 2000). This is the most simple and cheap way, but it is applicable only in areas with long Sunny periods and a slight temperature fluctuations.

However, in this method of cultivation there are drawbacks:

- inability took the treatment efficiency absorption of solar energy, because sunlight is the main limiting factor of biomass growth;

- in pools, which occupies a large area in order to achieve a certain degree of mixing, the thickness of the layer of suspension of microorganisms should be no more than 150 mm;

- obtained product is unstable chemical composition.

In countries with colder climates often use cultivation in photobioreactors. Photobioreactors have a different design. Can be a vertical glass column, the content of which is mixed with the air stream, enriched in CO2supplied through the bottom nozzle. A known method of cultivation in bioreactors in the form of a transparent plastic or glass tubes (http://www.bioprodukte-steinberg.de/index.php?op=algenfarm/ link to company website Bioprodukte Prof. Steinberg). The advantages of tubular reactors include the ability intense lighting, allowing it to achieve high biomass density, the possibility of continuous monitoring of gas exchange. At the same time, the constructive resolution of these devices complicates the implementation process with significant gas exchange. The negative impact of these deficiencies on the operation of the apparatus compounded with the increase in the ratio between the illuminated surface to the working volume of the reactor. All the light energy absorbed by the illuminated surface of the calling device and is not used in photosynthesis, is converted into heat.

Known methods of cultivation of phototrophs with the use of intermittent lighting using courses (Flash stimulation of plants. Edited by Shakhova A. M.: Nauka, 1971 - 368 C. Article FOV: Theoretical aspects of the transformation of light energy in a pulsed mode). However, application of the obturator does not reduce the cost of electricity.

Closest to the proposed installation implementing the method of cultivation of phototrophs is a shaker-incubator, New Brunswick Innova 42R (http://www.nbsc.com/flles/Innova_42.pdf - brochure of the firm, New Brunswick). In a shaker-incubator, New Brunswick Innova 42R transparent cultivators in the form of a series of vessels of the same geometric shape set with shaking by reciprocating movement (for aeration and mixing) in the horizontal plane and provided with a light source in the form of compact fluorescent lamps with light intensity above relative to the cultivators.

You can call the following disadvantages of this method. In the prototype used fluorescent lamps contain mercury, which creates the problem of disposal of these bulbs. Fluorescent lamps have a shorter life and lower efficiency compared with led sources osveshenie.otlichnie light in this way is at some distance, lighting not only the culture of phototrophs, but the environment around it, which reduces the overall efficiency of the entire installation.

The disadvantages include the inability to use in such a setting pulse mode light, light with a pulse duration of 0,00001 is 0.001 s, and the interval between pulses of 0.01-0.1 s, corresponding to the durations of light and dark phases of photosynthesis for phototroph, due to the technological characteristics of fluorescent lamps.

Object of the invention is the achievement of the technical result - the reduction of the energy consumption in the cultivation of phototrophs. The problem is solved in that in the method of cultivation of phototrophs, which consists in mixing and aeration of the culture liquid by shaking the flasks-cultivators by reciprocating movement, maintenance of set values of temperature, pH and the illumination light source according to the invention the light is a pulsed light source with a pulse duration of 0,00001 is 0.001 s, and the interval between pulses of 0.01-0.1 s, corresponding to the durations of light and dark phases of photosynthesis for phototroph. Installation for implementing the method includes cultivators in the form of a series of vessels of the same geometric shape with transparent bottoms, installed in what zmoznostjo reciprocating movement in the horizontal plane (for aeration and mixing) and is equipped with a light source, which according to the invention, the light sources is made in the form of a set of light emitting diodes located directly under the transparent bottoms of vessels and connected to the power source in the form of a pulse generator.

The invention is illustrated by figure 1 and 2, in which figure 1 shows a prototype, figure 2 is the requested installation. The installation includes the cultivator 1, a source of pulse lighting (light emitting diodes) 2 shaker 3 (for mixing and aeration by shaking by reciprocating movement of the platform with cultivators), the power - pulse generator 4.

Enablement of the claimed invention is shown in the following examples.

Example 1. The experiment was carried out at the facility, the scheme of which is shown in figure 2. The object of research was selected Chlorella sp., cultivation was carried out on the environment Tamiya within 10 days at a temperature of 35C. the Fermentation was cultivators (1) - Erlenmeyer flasks with a nominal volume of 250 ml with a volume of culture fluid 50 ml Mixing and aeration of the suspension was carried out by shaking by reciprocating movement of the cultivators of the shaker (3). The biomass growth was determined on the photocolorimeter ck-2. Illumination was carried out from the bottom. Each source pulse lighting (2) contains the 12 LEDs, power consumption of each is about 0.08 watts. The luminaires are connected to a power source, a pulse generator (4).

The experiment studied the following types of lighting: control (constant illumination received from fluorescent lamps) and experimental (dashed), with the duration of the light pulse 0,00001 with and the duration of the interval between light pulses of 0.01 C.

The result of the experiment to obtain 1 g of biomass in the control sample, the coverage was spent 8,21 Wh, experimental - 0.06 Wh

Example 2. The experiment was carried out in conditions similar to example 1, except that the duration of the light pulse amounted to 0.001 s, the interval between pulses was 0.1 C. the result of the experiment to obtain 1 g of biomass in the control sample was spent 8,21 Wh, experimental - 3,49 Wh

Thus, the method and plant for realizing it, can significantly reduce the cost of electricity for lighting during the cultivation of phototrophs and thus reduce the value of the final target product. The technical result is to reduce energy costs up to 0.06-3,49 Wh when receiving 1 g of biomass of phototrophs.

1. The method of cultivation of phototrophs, which consists in mixing and aeration of the culture fluid by vstrechi the project due to the reciprocating movement of the cultivators, maintaining desired values of temperature, pH, illumination light source, wherein the illumination is carried out by a pulsed light source with a pulse duration 0,00001 is 0.001 and the duration of the interval between pulses of 0.01-0.1 s, corresponding to the durations of light and dark phases of photosynthesis for phototroph.

2. Installation for implementing the method according to claim 1, including cultivators in the form of a series of vessels of the same geometric shape with transparent bottoms, in which mixing and aeration are shaking by reciprocating movement in a horizontal plane, and provided with a light source with adjustable intensity of light, wherein the light sources are made in the form of a set of light emitting diodes located directly under the transparent bottoms of vessels and connected to the power source in the form of a pulse generator with adjustable frequency and duration of the light pulse.



 

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