Method of automatically governing biotechnological process

FIELD: biotechnology and microbiological industry.

SUBSTANCE: invention concerns governing periodical air-intake biotechnological process carried out in bioreactor. Method comprises measuring oxygen content in effluent gas, working volume of culture medium, concentration of biomass, and concentration of intermediate product of its vital activity. Measured parameters allow specific oxygen consumption rate and velocity of intermediate product concentration change to be determined to enable regulation of feeding air used in aeration, supplying nutritional medium, and agitating culture medium. Moreover, temperature of culture medium, temperature of supplied and withdrawn cooling agent, and consumption of the latter are measured to use these parameters for determining biomass heat release rate and velocity of intermediate product amount change. The two latter parameters enable regulation of feeding air used in aeration and supplying nutritional medium. The following characteristics are thus improved: elevating power by 8.1%, maltase activity by 7.9% and resistance by 7.4%.

EFFECT: enhanced efficiency of governing biotechnological process and improved qualitative characteristics of process.

2 ex

 

The invention relates to the microbiological industry, and can be used to control the periodic air-inlet biotechnological process in the bioreactor.

The proposed method can be applied to industrial control and experimental biotechnological processes using microorganisms capable of producing and consuming intermediate product life, such as Saccharomyces cerevisiae, Acetobacter, Lactobacillus, Zymomonas and other

There is a method of automatic control of biotechnological processes (A.S. No. 1062262, MKI 12 Q 3/00), namely, that measure the oxygen content in the exhaust gas and the working volume of the culture medium, to determine the specific growth rate of microorganisms and regulate the flow of air for aeration and flow of the nutrient medium with the specific growth rate of microorganisms.

The disadvantage of this method is low efficiency of regulation and control accuracy of qualitative indicators as specific growth rate of microorganisms is not sufficiently informative indicator, and the error of its determination unacceptably high.

The closest in technical essence and the achieved result is a method of automatic control of biotechnological processes (A.S. No. 1786072, 10.07.90, IPC5C 12 Q 3/00), allcauses is to measure the oxygen content in the exhaust gas, the working volume of the culture medium, the concentration of the intermediate product resulting from biomass and the biomass concentration, determined according to the measured parameters, the specific rate of oxygen consumption and the rate of change of concentration of the intermediate product and regulate the flow of air for aeration, the flow of the nutrient medium and mixing of the culture medium on the specific rates of oxygen consumption and the rate of change in concentration of the intermediate product.

The disadvantage of this method is low efficiency of regulation and control accuracy of qualitative indicators, as the accuracy of determining the specific rate of oxygen consumption of unacceptably high due to the low accuracy of measurement of the oxygen content in the exhaust gas, and the rate of change of concentration of the intermediate product is insufficiently informative in terms of variable working volume of the culture medium when the concentration of the intermediate product may be caused not by the consumption of the product biomass, and dilution due to the supply of the nutrient medium.

The present invention is to increase regulatory effectiveness and the accuracy of control of quality indicators.

This object is achieved by those who, a method of automatic control of biotechnological process involving the measurement of the oxygen content in the exhaust gas, the working volume of the culture medium, the measurement of the biomass concentration and the concentration of the intermediate product of its activity, the definition of the measured parameters of the specific rates of oxygen consumption and the rate of change in concentration of the intermediate product and the regulation of air supply for aeration, feed nutrient medium and mixing of culture medium, the difference is that additionally measure the temperature of the culture medium, the temperature of the nutrient medium, the temperature and exhaust refrigerant flow rate and the measured parameters to determine the specific rate of heat biomass and the rate of change of the quantity of the intermediate product, and regulation of air supply for aeration and feeding of the nutrient medium is carried out in dependence on the specific rate of heat biomass and the rate of change of the quantity of the intermediate product.

Additional measurement of the indicated parameters (temperature, culture medium, the temperature of the injected medium, the inlet temperature and exhaust refrigerant flow rate) is necessary to determine the optimal conditions W is snipealot biomass and the rate of change of the quantity of intermediate product for the regulation of these indicators air supply for aeration and feeding of the nutrient medium.

The method is as follows. During biotechnological process in the bioreactor with biomass provide air to the aeration and nutrients. The process of fermentation biomass is accompanied by heat. To maintain the optimum temperature activity of the biomass it periodically cooled.

During biotechnological process may develop populations for the following routes of metabolism, the first of which is most favorable from the point of view of regulatory effectiveness and level of quality indicators, and the rest correspond to the major manufacturing deviations, resulting in deterioration of quality:

1. breath on the main substrate or oxidation growth corresponds to the best quality indicators: large specific heat release rate of biomass, the rate of change of the quantity of the intermediate product of zero and the maximum yield of the target product;

2. breath on the main substrate and the intermediate product (simultaneous consumption) - corresponds to the insufficient supply of the nutrient medium. The specific heat release rate of biomass decreases, the rate of change of the quantity of the intermediate product is negative, and the output of the target product decreases slightly;

3. breathing in the intermediate product with the full exhaustion of the main substrate corresponds to the insufficient supply of the nutrient medium. The specific heat release rate of biomass is further reduced, the rate of change of the quantity of the intermediate product is negative, and the output of the target product is significantly reduced;

4. aerobic fermentation corresponds to overdose nutrient medium. The specific heat release rate of biomass is further reduced, the rate of change of the quantity of the intermediate product is greater than zero, and the output of the target product;

5. anaerobic fermentation at high concentrations of the main substrate corresponds to simultaneous overdose nutrient medium and there is insufficient air for aeration. The specific heat release rate of biomass is further reduced, the rate of change of the quantity of the intermediate product is greater than zero, and the output of the target product;

6. anaerobic fermentation at low concentration of the primary substrate corresponds to an insufficient supply of air for aeration. The specific heat release rate of biomass is further reduced, the rate of change of the quantity of the intermediate product is greater than zero, and the output of the target product;

7. complete cessation of growth of the biomass due to insufficient air supply for aeration (in culture medium no main substrate or intermediate about the ukta, not enough oxygen). The specific heat release rate of biomass is minimal and the rate of change of the quantity of the intermediate product and the yield of the target product is equal to zero.

The effectiveness of regulation can be assessed, for example, by total biomass yield (target product) from the main substrate. Qualitative indicators are, for example, such indicators of biomass as a lifting force, maltana activity and durability. The maximum value of biomass yield correspond to the best quality.

Determining the specific rate of heat biomass and the rate of change of the quantity of the intermediate product, you can clearly identify one of the six above-listed types of process deviations and implement appropriate regulatory action.

As examples used biotechnological process of cultivation of Baker's yeast for 13 hours in the bioreactor Poland-200 capacity 200 m3. The initial biomass concentration was 5 kg/m3. The initial substrate concentration was 0.5 kg/m3. The initial volume of culture medium was 70 m3. The concentration of the primary substrate (sugars) in the feed was 300 kg/m3. In the course of the process in the bioreactor was continuously supplied air is aratzio and nutrient medium. Mixing of the culture medium was carried out aerating air.

Example 1. The proposed method was implemented on the prototype. When this air supply for aeration and feeding of the nutrient medium was produced in accordance with the technological regulations, i.e. the regulation of the air supply for aeration and feeding of the nutrient medium was carried out in an automatic mode using as jobs regulators technological temporary programs. Correction programs carried out by the specific rate of oxygen consumption and the rate of change in concentration of the intermediate product (ethanol). During the process took place technological deviation of 2, 4, 5, 6.

Upon completion of the process yield of the target product (biomass yeast), which is a measure of the effectiveness of the regulation was 0.45, and the quality indicators respectively: lifting force to 40 minutes, maltana activity - 41 min, resistance - 121 PM

Example 2. The process was managed in accordance with the proposed method. In the process for short periods of time occurred technological deviation of 2 and 4.

Upon completion of the process yield of the target product (biomass yeast), which is a measure of the effectiveness of regulation, was 0.49, and the quality indicators respectively: lifting force 37 minutes, maltana activity - 38 min, a hundred and the bone - 130 hours

The use of the proposed method in comparison with the prototype allows to increase the efficiency of regulation, the measure of which is the output of the target product, 8.9% and to increase the accuracy of control of the quality indicators due to the fact that the accuracy and reliability of measuring temperature and flow greatly exceeds the accuracy and reliability of detection.

The proposed method will improve quality indicators: lifting force, 8.1%, maltezou activity - by 7.9%, resistance - by 7.4%.

Method of automatic control of biotechnological process involving the measurement of the oxygen content in the exhaust gas, the working volume of the culture medium, the measurement of the biomass concentration and the concentration of the intermediate product of its activity, the definition of the measured parameters of the specific rates of oxygen consumption and the rate of change in concentration of the intermediate product and the regulation of air supply for aeration, feed nutrient medium and mixing of culture medium, characterized in that it further measure the temperature of the culture medium, the temperature of the nutrient medium, the temperature and exhaust refrigerant flow rate and the measured parameters to determine the specific rate of heat biomass is the rate of change of the quantity of the intermediate product, and regulation of air supply for aeration and feeding of the nutrient medium is carried out in dependence on the specific rate of heat biomass and the rate of change of the quantity of the intermediate product.



 

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FIELD: automatic malting process.

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FIELD: biotechnology and microbiological industry.

SUBSTANCE: invention concerns governing periodical air-intake biotechnological process carried out in bioreactor. Method comprises measuring oxygen content in effluent gas, working volume of culture medium, concentration of biomass, and concentration of intermediate product of its vital activity. Measured parameters allow specific oxygen consumption rate and velocity of intermediate product concentration change to be determined to enable regulation of feeding air used in aeration, supplying nutritional medium, and agitating culture medium. Moreover, temperature of culture medium, temperature of supplied and withdrawn cooling agent, and consumption of the latter are measured to use these parameters for determining biomass heat release rate and velocity of intermediate product amount change. The two latter parameters enable regulation of feeding air used in aeration and supplying nutritional medium. The following characteristics are thus improved: elevating power by 8.1%, maltase activity by 7.9% and resistance by 7.4%.

EFFECT: enhanced efficiency of governing biotechnological process and improved qualitative characteristics of process.

2 ex

FIELD: chemistry; biochemistry.

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