Method of monitoring microbiological process in the fluid flow (options)
(57) Abstract:The invention relates to pharmaceutical and biotechnological production, and can also be used in wastewater treatment, production using fermentation and fermentation. The method involves the measurement of pH and dissolved oxygen in the liquid sample at selected time intervals. Then determine the rate of change of pH and the rate of biological oxygen consumption. Fix metabolically important transitional point, they will judge the status of microbial populations and the content of organic and inorganic substances in the sample or determine the time of nitrification. The method has a wide application area, less time consuming and provides high efficiency of the process being monitored. 3 S. and 19 C.p. f-crystals. 13 ill., table 1. The invention relates to a method of monitoring metabolically significant transition points during microbial metabolism of organic and inorganic substances and regulation of microbiological process.THE EXISTING LEVEL OF TECHNOLOGY
Microbial use of organic and inorganic substances in metabolic processes can the ptx2">If nitrification is the dominant reaction in microbial culture, it can be expected that obtaining hydrogen ions (H+in the nitrification process will be significantly reduced by reducing the number of commonly used ammonium (NH4+below a certain critical metabolic level. Therefore, it can be expected that the activity of hydrogen ions in solution, i.e., the pH will also change.Similarly, it is expected that the consumption of oxygen microbial culture will be higher in the case when in the presence of a large number of exogenous organic matter than in the case when the amount of these substances is below a certain metabolically significant level. In both cases, the measured rate of change of pH, which is hereinafter sometimes referred to as "receive rate pH" or "pHPR", and the flow of oxygen, which is hereinafter sometimes referred to as "the rate of biological oxygen consumption" or "BOCR, will directly determine the rate of metabolism of the substance over time. Thus, if we assume that changes in pH and oxygen in the environment are the result only of microbial metabolic activity, pHPR and BOCR theoretically can be is determined as d(pH)/dt or -(pH)/t, and BOCR is defined as d(DO)/dt or -(DO)/t. A negative increment of pH and/or DO has resulted in the measurement of positive pHPR and/or BOCR.SUMMARY OF INVENTION
The method of the invention includes the selection of the sample liquid from the liquid source, for example, the wastewater in the treatment process. pHPR is calculated by measuring the pH of the liquid sample, and analyzed for the rapid determination of the occurrence of significant metabolic transition points. The analysis shows what stages of regulation are required and when they must be implemented to ensure the maximum efficiency of the process being monitored.BRIEF DESCRIPTION OF DRAWINGS
Fig. 1 graphically presents theory of reaction kinetics Mikelis-Menten.In Fig. 2 shows a graph of theoretical dependence of the rate of oxygen consumption (BOCR) and the rate of change of pH (pHPR) sample of the mixed liquid as concentrations of ammonia (NH4+and organic carbonaceous materials, collectively referred to as BOD (biochemical oxygen demand), from time to time in microbiological process.In Fig. 3 shows theoretical dependence of the rate of energy consumption is UP>+and organic carbonaceous materials, collectively referred to as BOD (biochemical oxygen demand), from time to time in microbiological process.In Fig.4 schematically shows a vertical projection device according to one variant of the invention, which can be used for the selection and monitoring of a sample of fluid from the fluid flow in the vessel of the bioreactor in accordance with this invention.Fig.5 graphically illustrates the relationship between the rate of change of the amount of oxygen in the period between cessation and onset of aeration and BOCR, expressed as the percentage change in oxygen saturation in a minute.Fig. 6 graphically illustrates the relationship between the change of the pH value during the period between cessation and onset of aeration and pHPR expressed as the change in pH per minute by changing the concentration of ammonia.Fig.7 graphically shows the relationship between pHPR expressed as the change in pH per minute, and the concentration of ammonia, where COD is not metabolically limiting factor.Fig.8 graphically shows the relationship between BOCR, expressed as the percentage change in oxygen saturation in mine which indicates changing pHPR, expressed as the change in pH per minute, under different conditions the presence of ammonia and COD.Fig.10 shows the relationship between pHPR expressed as the change in pH per minute, BOCR, expressed as the percentage change in the oxygen saturation per minute, the concentration of ammonia and COD under various conditions in the presence of ammonia and COD.Fig. 11 is a graph showing changes in DO and pH with respect to time at constant aeration.Fig. 12 is a graph showing the pH, the concentration of NH3-N and d(pH)/dt with respect to time.Fig.13 is a graph of DO and d(DO)/dt with respect to time.THE INVENTION
Mechanical rate at which biochemical reactions occur, can be partially described by theory Mikelis-Menten, as illustrated in Fig.1. According to this theory, the speed of biochemical reactions is very low at very low concentrations of substances, but the rate increases with increasing concentration of the substance to the point, above which it will rise slightly, regardless of the magnitude of the growing concentration of the substance. In other words, regardless of how much to increase the concentration of a substance above this point is to place a maximum reaction rate or Vmax. This linear extrapolation corresponding to the concentration of the substance is equal to 2Ks. Ksis the concentration of substance at which the rate of metabolic reactions is half the maximum reaction rate (Vmax).It follows that, from a metabolic perspective, 2Ksis a significant concentration of a substance. Microbial metabolism of the substance continues at concentrations above 2Ksat the maximum, and almost constant speed. The rate of metabolic reactions can be variable and limited availability of substances less than 2Ks. Therefore, you can expect changes some of the parameters measured, which is directly connected and related to the rate of microbial metabolism of specific inorganic and organic substances, due to changes in the concentration of specific substances. Characteristically, when the concentration of the substance is equal to or more than 2Ks, it is expected that the dependent measured parameter and/or the measured rate of change of this parameter over time will be relatively constant. When the concentration is reduced to below 2Ks, it is expected that the dependent measured parameter and/or the in, measured when the concentration of the substance is equal to or above 2Ks.For many biological reactions, it is desirable to determine the point at which the concentration of specific substances was below this metabolically significant concentration 2Ks. It is possible to determine changes in the model of the metabolic behavior of microbial culture by monitoring changes in certain dependent measured parameters when changing the concentrations of certain organic and inorganic substances.For example, in many processes of wastewater treatment is required to reduce the concentration of some organic and inorganic substances to very low levels. These substances typically include such organic substances that can generally be considered and measured as BOD (biochemical oxygen demand) or COD (chemical oxygen demand), and inorganic ammonium (NH4+). Assuming that the nitrification reaction and the reaction of reduction of BOD/COD were the two most dominant reactions can be expected characteristic changes in the rate of oxygen consumption (BOCR), and the rate of change in pH (pHPR) as BOD and ammonia were below their matched with the tsya, what a lengthy process wastewater of pH and DO in the liquid environment depend on many factors, such as the concentration of nutrients (biodegradable carbonaceous, nitrogenous, phosphorus compounds, etc.), biomass concentration, alkalinity, etc., These factors are constantly changing, while the waste water passes through the treatment equipment. Therefore, it is difficult to establish the relationship between the measured parameters and implementation of wastewater treatment due to mutual dependencies too many unknown and constantly changing factors. If only these interacting factors can be either defined or maintained at a constant level when measuring pH and DO measurement pHPR and BOCR will not provide more valuable information for the implementation of wastewater treatment.The use of the device for determination of biological activity are described in U.S. patent 5466604, allows the selection of in situ samples of wastewater from the main volume of wastewater during cleaning. Of course, can be used, and other devices in accordance with this invention. The term "in situ" is also used here to describe any method you who IU liquid, for example wastewater. In other words, there may be used a device that physically removes a sample (samples) from the main volume of the liquid at such a period of time that measurements can be made, in essence, "real-time" and "online".Theoretical response BOCR and pHPR on changes in the concentration of BOD and ammonia (NH4+) shown in Fig.2 and 3 and described below. The figures graphically presents the response of a single sample of the mixed liquid (i.e., wastewater) and microbes for biological Department of nutrients (BNR), isolated from the main volume of wastewater. The selected alternative sample is exposed and not exposed to aeration. Aeration begins and continues until it achieves the level of dissolved oxygen, which, with some margin, higher than the level of DO in the bulk wastewater. Upon reaching this level, the aeration is stopped and begins only when the level of dissolved oxygen in the sample reaches the level that with some margin below the level of DO in the bulk wastewater. During those periods when the aeration is not performed, BOCR, and pHPR determined and rasschitat what Sloboda, expressed as percentage saturation, measured over a period of time t, and
pHPR = -(pH)/(t)
where pH is equivalent to the change observed in pH during time period t.
As shown in the period a in Fig. 2 and 3, when the concentration as NH4+and BOD, above their respective values 2Ks, BOCR is constant at its highest relative level, as the use of BOD continues at maximum speeds and prevails over the nitrification reactions with oxygen depletion. Thus, pHPR is constant at the average level. This model BOCR/pHPR, as described above, it is expected, if we assume that 1) the reaction of nitrification and use of BOD are the dominant reactions in the biological sample, 2) production and activity of hydrogen ions associated with the reaction rate of nitrification, and 3) the reaction is not limited by the presence of oxygen.Subsequently, the continued metabolism reduces the content of existing NH4+below its value 2Ksand nitrification rate, hydrogen ions decreases from the maximum speed to a lower speed, when the concentration of ammonia is metabolically limiting factor. As pocataligo level, reflecting reduced demand and consumption of oxygen, due to the comparatively low reaction rate of nitrification. Changes in the concentration of ammonia from values above 2Ksto a value below 2Ksdepicts the transition between periods a and b in Fig.2.The period in Fig.2 and 3 shows that when the concentration of the available NH4+below its value 2Ksand after reducing BOD below its value 2Ks, pHPR increases very slowly, to reflect the change in pure metabolic behavior of mixed biological populations, and BOCR falls to its lowest speed to reflect the very low consumption of oxygen by reaction with a flow rate of BOD and nitrification. This transition is depicted between periods b and C in Fig.2.The period D in Fig. 3 shows that when the BOD concentration below its size 2Ksand the concentration of NH4+above its size 2Ks, pHPR increases to its highest level, reflecting the high rate of nitrification and BOCR falls to an average level, reflecting a net decrease in the total consumption of oxygen due to reactions with reduced flow BOD. Higher pHPR observed under this condition, because the buffer the effects of reaction is waista the sample through a system of carbonic acid. Thus, in the absence of reactions with a flow rate of BOD and obtain as a consequence, the CO2, pHPR is much greater than in other conditions.It is possible to identify relevant information regarding a biological sample on the basis of the above example by monitoring and comparing patterns and/or levels BOCR and pHPR, because they represent key measured the dependent parameters of microbial metabolic activity. Specifically, this example illustrates how to determine: 1) if nitrification and BOD removal at the same time at maximum speeds, 2) does nitrification, when BOD decreased to levels below its value 2Ks, 3) continue whether the reaction of removal of BOD, when the amount of ammonia decreased to below its value 2Ksand 4) decreased if the number and ammonia and BOD to below their respective values 2Ks.Immediate and constant comparison of measured parameters BOCR and pHPR leads to several conclusions regarding the status of wastewater. If the sample is mixed liquid is subjected to constant monitoring, and a significant increase in pHPR occurs simultaneously with a decrease in BOCR, it alasse many more. If the sample is mixed liquid is subjected to constant monitoring, and BOCR is reduced to the average level, while pHPR is reduced almost to zero, this indicates that the ammonia content decreased to below its corresponding value 2Kswhile the BOD is still significant. If the sample is mixed liquid is subjected to constant monitoring, and BOCR is reduced to a low level and as pHPR is reduced to a low level, it indicates that the content of ammonia and BOD was reduced to levels below their respective values 2Ks. This condition also indicates a decrease BOCR to a low level and a slight increase pHPR from approximately zero level to a slightly higher, but, in fact, low.The table summarizes these characteristics and illustrates how the comparison of the respective values and characteristics of the measured parameters BOCR and pHPR leads to obtaining the relevant information described above in connection with Fig.2 and 3.In Fig.4 shows an example of a preferred device used to allocate the sample of wastewater. The device 11 is immersed in the bath 2 (shown only a part of it) with the waste water includes definitive Kam 56, powered by Acme rod 57 connected to the motor 53. In the open position, rotation of the screw 48 provides for the exchange of waste water in the chamber 8 between the inward and outward directions definitive camera, and a key to the chamber 8 is filled with a new sample of wastewater. After a preset period of time, for example 30 seconds, the programmed motor 53 changes the direction of rotation, the movable cover 32 is pulled in the direction of arrow "b" to complete closure and sealing definitive chamber 8. The movable cover 32 and the screw 48 is driven by the same low-speed reversible motor 53, which coaxially connects the inner rod 56 and the outer rod 55. The coaxial connection is enclosed in the tube 54 made of stainless steel.The DO concentration is determined by the probe (10) after filling the definitive camera 8 new sample of wastewater, and if DO is less than the oxygen concentration in the primary effluent to a predetermined reserve amount, the air and/or oxygen is blown in the direction of the guide chamber 8 through the aeration pipe 13 to achieve such concentrations DO. The concentration of DO at a level that is higher or lower than the oxygen concentration in the bulk sewage on the school camera 8 will be the same as or similar to the process of nutrient removal in the primary effluent. In this way the probe 12 pH detects changes in pH. In addition, the screw 48 may be periodically or continuously rotate to maintain the sample in the well-mixed or suspended.The aeration device 11 is interrupted for a certain time interval for measurements after reaching maximum concentrations DO. During this period, the residual concentration of DO and pH, which in General is not affected by the aeration tanks with sewage, monitored using probes. Signals about the pH and residual DO from the respective probes 12 and 10 are sent to the controllers that convert changes in DO in relation to the time in BOCR, and changes in pH with respect to time in pHPR by numerical differentiation in accordance with the above equations.In most installations for wastewater treatment, the concentration of BOD and ammonia after the final cleaning is below the values 2Ksfor BOD and NH4+. When the BOD concentration and NH4+in determinative the chamber is reduced below the values 2Ksaerobic metabolic reactions for nutrient removal are completed with significant changes in LVEF is Elena with BOCR analysis and pHPR in accordance with the criteria shown in the table. For other biological processes of concentration of substances in the environment are usually significantly higher than the values 2Ksto maintain maximum speed and obtain the desired substance. Thus, determination of the complete metabolic reactions will signal the need to add nutrients, or about the time of the termination of the biological process, or about the time of collecting analyte obtained during the process.Information about aerobic metabolic reactions for removal of nutrients, such as completion time (NT) nitrification, denitrification (DNT), and so on, can be used for regulation and control of process wastewater and other aerobic metabolic processes. For example, the measured NT can be compared with an average hydraulic retention wastewater in the aeration tank in the effluent treatment plant. If NT is significantly less than the time hydraulic retention HRT, aerobic nutrient removal completed in the middle of the aeration tank. After that, the rest of the aeration tank is, in fact, inoperative and is not involved in the process of cleaning hundred is erotiska of operation for operating costs and/or (2) to accept a larger volume of wastewater and effectively increase the performance of the treatment plant, and/or (3) reduce the amount of air fed into the aeration tank, to reduce the rate of aerobic metabolic reactions to NT exactly HRT in the aeration tank and reduced energy consumption from the discharge fan.EXAMPLE 1
The mixed sample fluid taken from the aeration tank of an improved installation for the biological treatment of wastewater, located in oaks, Pennsylvania, was highlighted in the tank, equipped with devices for measuring pH levels, and saturation of dissolved oxygen, as well as devices for aeration and maintain the sample in the well-mixed condition. Data from devices that measure the levels of pH and saturation of dissolved oxygen was recorded and analyzed by computer to calculate the BOCR and pHPR. Then alternated periods of time when the sample was subjected or not subjected to aeration. Aeration was begun and continued up until not achieved this level of dissolved oxygen, which was comparable to its level in the bulk wastewater plus a certain margin in the allocation of the sample. Concentrations of NH4+and soluble carbonaceous organic matter was measured and represented as C and BOD. During periods of aeration, examples of which are marked by arrows in Fig.5 and 6, as BOCR and pHPR was calculated and was calculated by numerical differentiation, as described above.In Fig.5 shows the saturation of dissolved oxygen and BOCR in the study period, when the measured concentration of COD was consistently larger than 150 mg COD / l, which is significantly higher than the values 2Ksfor COD, but when the concentration of ammonia was changed from the values above 2Ksto values below 2Ks. Fig. 5 discloses the relationship between the raw data on dissolved oxygen, which represent the rate of change of the amount of oxygen in the period between cessation and onset of aeration, as indicated, and BOCR. Fig.5 also illustrates the transition in the level BOCR from high to medium during metabolically significant transition, when the concentration of ammonia is reduced below its value 2Ks. BOCR expressed as the percentage change in oxygen saturation in a minute.In Fig.6 shows the pH and pHPR sample for the same period as in Fig.5. During this period the measured COD concentration was always greater than 150 mg COD / l, which was significantly above the values 2Ksfor COD, but the concentration of ammonia and the IDE between raw data about the pH level, i.e. change of the pH value during the period between cessation and onset of aeration, as indicated, and pHPR. Fig.6 also illustrates a shift in pHPR from mid-level to about zero during metabolically significant transition, when the concentration of ammonia is reduced below its value 2Ks. pHPR is expressed as the change in pH per minute.In Fig. 7 shows the change in the measured levels of ammonia and calculated pHPR over the same period, as shown in Fig.6. Fig.7 illustrates the transition in pHPR from the average level to near zero during the transition ammonia concentrations from magnitude approximately equal to the value 2Ksto a level below the value 2Ks. pHPR is expressed as the change in pH per minute.In Fig.8 shows the changes in measured levels of ammonia and calculated BOCR over the same period, as shown in Fig.5. Fig.8 illustrates the transition BOCR from high to medium level during the transition ammonia concentrations from levels above 2Ksto below 2Ks. BOCR expressed as the percentage change in oxygen saturation in a minute.Fig.9 graphically shows the constancy based pHPR from ammonia concentrations. This was achieved by adding a solution of ammonia in the mixed sample fluid in those moments, CT=195 min, COD concentration was significantly higher values 2Ks. About at T=90 min a significant transition can be observed in pHPR, when the concentration of ammonia decreases to below its value 2Ks.Subsequent addition of ammonia were made at T=120 min and T=170 min, when the pH was around zero. Fig.9 shows that pHPR jumped sharply to almost zero level immediately before each subsequent addition to a relatively average level that was observed between T= 0 min and T=90 min After subsequent additions of ammonia pHPR returned to almost zero or low level after reduction of the ammonia content below 2Ks. COD were present in sufficient quantity, and the reduction of the ammonia content decreased pHPR almost to the zero level in the case of the first addition of ammonia at T=120 min Reduction of ammonia occurred at a time when the COD concentration was reduced to below its value 2Ksin the second case, the addition of ammonia at T= 170 min In the pHPR decreased to low, but not zero level, as shown in the period in Fig.2 and 3.Fig. 10 gives a more complete picture of the data shown in Fig.9, and includes calculated pHPR calculated the adequate levels in the case of significant metabolic effects.You can quickly and accurately determine the moment when the concentration of organic substances and/or inorganic substances falls below their respective levels 2Ksas shown in this example, by monitoring the appropriate levels BOCR and pHPR, in accordance with this invention. Determination of reducing the number of specific substance below its corresponding and significant metabolic concentrations 2Ksoften shows a significant change in the state of the microbial population or its environment, or a change in the metabolic model and/or the behavior of the sample containing the active microbes.In response can be taken various regulatory actions depending on the specific process. For example, reducing the amount of a specific substance in microbial populations may signal a change in metabolism, so that it is possible to obtain the desired secondary metabolite, which indicates that the process should continue until the stage of separation, collection and/or purification. Similarly, in biological processes, the aim of which is to maintain the concrete scheme of stepwise feed of the substance in the microbial population, the ability to Opredelenie the concentration of a substance above 2Kscan be used to indicate that it is desirable to increase or decrease the supply of the substance.In Example 1 described aerobic biological wastewater treatment, the aim of which is often reduced through biological mechanisms of the amount of specific inorganic and organic substances, for example the reduction of soluble ammonia and carbonaceous organic substances. Thus, various regulatory actions can be taken in response to the reduction in the number of one or more of these substances below the level of concentration 2Ksas the concentration 2Ksoften below the low level of concentration required for many substances. For example, if you discovered that the concentration of organic and inorganic (ammonia) substances below their respective concentrations 2Ksthe flow velocity in wastewater treatment processes can be increased, which will increase treatment capacity. If found that the concentration of organic and inorganic substances above their respective concentrations 2Ksthe flow velocity in wastewater treatment processes can be reduced. If the content of Emesene due to reduced demand in nitrification. Finally, if the content of organic matter below the value 2Ksand ammonia is higher than the value 2Ksthe aeration can be increased to create more favorable conditions for nitrification.EXAMPLE 2
Example 2 a sample of the mixed liquid was separated in the same manner as described in Example 1. Constant aeration of the mixed sample fluid was maintained throughout the period during which the sample was selected. The rate of aeration was chosen so that the level of dissolved oxygen concentration in the sample was higher than the critical value required for biological removal of carbon nutrients and ammonia. Changes in oxygen concentration and pH were monitored using a probe dissolved oxygen and pH probe, as shown in Fig.11.Then a small amount of the mixed liquid was periodically allocated from the selected sample, and tested the concentration of ammonia. Fig.12 shows changes in the concentrations of ammonia and pH throughout the aeration period, during which the sample was selected. The end of nitrification (ammonia was lower than the detection level, i.e. 0.1 ppm) was accompanied IU the of ammonia close to zero, the value of d(pH)/dt has passed the second zero point. Characteristic d(pH)/dt in the second zero point can also be defined as the point where d(pH)/dt changes from negative values to zero. The time corresponding to this point is defined as the completion time of nitrification mixed liquid, or NT. In Example 2, as shown in Fig. 12, NT is measured at about 75 minutes. Dimension d(pH)/dt in Example 2 differs from the measurement in Example 1. In Example 1 d(pH)/dt was measured in the absence of aeration, whereas in Example 2, d(pH)/dt was measured with continued aeration. Due to the constant removal of CO2from the mixed liquid, it is possible to see the decrease in pH during the measurement. Thus, pHPR is sometimes negative.In Fig. 13 shows the curve of dissolved oxygen and its derivative, d(DO)/dt, for the same sample. When ammonia was used, the value of the first derivative of DO, d(DO)/dt, began to increase significantly. The time value of nitrification (NT) measured for DO, also accounted for approximately 75 minutes.Next will be described one practical application of NT measurement in regulating the biological process of nitrification. In one series of bioreactors, where the biological process nitrify the unintended NT shows when the existing biomass concentration and the dosage of ammonia will take time NT complete nitrification.Time hydraulic retention (HRT) of the mixed liquid in one of a series of bioreactors is calculated, taking into account the flow rate and flow regime of the mixed fluid and the geometry of the bioreactor. Then NT is compared with the time hydraulic retention of the mixed fluid. Should the nitrification process will have comparable values of NT and HRT in the daily work. When NT is much less than HRT, nitrification ends in a bioreactor or in some bioreactors previously given HRT, which means the process has additional capacity for nitrification. When other contaminants are removed before the ammonia is completely nitrification, the definition of the NT signals the end of the wastewater treatment process. This indicates that the process can be handled more wastewater in the given volume of the tank when the same operation, or that in the process you can use a smaller tank and to make certain savings in operating costs.On the other hand, if NT is greater than HRT, the concentration of ammonia Bud the treatment plant, increase the speed of aeration in the bioreactor (bioreactor) and/or the concentration of the mixed liquid. If NT is greater than HRT for a long period, this indicates that the process is overloaded for the removal of ammonia, and, most likely, the capacity of the equipment must be increased to handle this volume of wastewater.In General, when comparing NT and HRT such information, as the ability of the process to nitrification, the required speed of aeration in the bioreactor or the number of bioreactors and the quality of the effluent from the bioreactor effluent can be determined and sent to the operator to regulate the process of nitrification.This invention can be applied to any type of microbiological process, including (but not limited) wastewater treatment (urban, industrial, etc), pharmaceutical/biotech manufacturing, fermentation, fermentation or other processes involving pure or mixed populations of microorganisms. 1. Method of monitoring microbiological process in the liquid stream containing the microbial population, providing steps (a) selection of sample fluid from okazuya the rate of change in pH for a given sample, d) measuring the amount of dissolved oxygen in the specified sample of the liquid in the selected time intervals, in essence, synchronously with the specified pH, and (e) determine the speed of the biological oxygen consumption for a given sample, (f) repeating steps b) to e) in the selected time intervals and q) comparing again a certain speed (velocity) changes in pH and speed (velocity) of biological oxygen consumption with the previously defined speed (velocity) changes in pH and speed (velocity) of biological oxygen consumption.2. The method according to p. 1, wherein the definition of said rate of change of pH is carried out in accordance with the following formula:
where pHPR - specified rate of change of pH;
dpH is the change in pH;
dt - change time
dpH and dt close to zero.3. The method according to p. 1, characterized in that the measurement of pH and dissolved oxygen provide essentially continuous.4. The method according to p. 1, wherein determining said speed of biological oxygen consumption produced in accordance with the following formula:
where BOCR - specified NEC time;
and dDO and dt close to zero.5. The method according to p. 1, wherein comparing the specified again a certain speed (velocity) changes in pH and the rate (speed) of biological oxygen consumption with the previously defined speed (velocity) changes in pH and speed (velocity) of biological oxygen consumption to determine whether the levels of organic and inorganic compounds in the specified thread above or below their respective concentrations 2Ks.6. The method according to p. 1, characterized in that the step of allocating the specified liquid sample is carried out in situ.7. The method according to p. 1, characterized in that the phases of the measurement of pH and dissolved oxygen is carried out in the liquid sample containing the desired amount of dissolved oxygen.8. The method according to p. 1, characterized in that the liquid sample is isolated in a chamber for a liquid sample, comprising a diffuser for supply air and/or oxygen in the specified sample fluid and a device for mixing the sample.9. The method according to p. 8, characterized in that it further includes aeration of the specified liquid sample is specified aerator in the continuation of ml and pH in the specified pattern measure essentially continuous with constant stirring of the sample.10. The method according to p. 8, characterized in that it further comprises the steps of aerating the specified liquid sample is specified aerator to achieve the specified sample fluid desired level of saturation of dissolved oxygen before the steps of measuring pH and dissolved oxygen in the specified sample of the liquid and periodically or continuously mixing the specified pattern in the specified device for mixing during measurement of pH and dissolved oxygen in the specified sample of the liquid.11. The method according to p. 1, characterized in that the microbiological process selected from the group consisting of wastewater treatment, pharmaceutical production and production using fermentation.12. Method of monitoring microbiological process in the liquid stream containing the microbial population, providing steps (a) selection of sample fluid from the specified fluid flow; (b) measuring the pH of the specified sample fluid at selected time intervals, (c) determine the speed of establishing pH for the specified sample, d) display the results of this determination and repetition of steps a) to C) at selected time intervals and comparing the pH value, e) determining when the speed of establishing the pH is changed from a negative value to zero and/or reaches a zero value for the second time, which is judged on time nitrification and/or stages: (f) measuring the amount of dissolved oxygen in the specified sample of the liquid in the selected time intervals synchronously with the specified pH, g) determine the speed of the biological oxygen consumption for a given sample, (h) repeating steps f) and g) at selected time intervals and compare again a certain speed (speed) biological oxygen consumption with the previously determined speed of the biological oxygen consumption, i) determining when the rate of biological oxygen consumption begins to increase significantly, which is judged on time nitrification.13. The method according to p. 12, wherein determining said speed setting of the pH is carried out in accordance with the following formula:
where pHPR - specified speed setting pH;
dpH is the change in pH;
dt versus time;
dpH and dt close to zero.14. The method according to p. 12, characterized in that the specified izmerenii dissolved oxygen to determine said speed of biological consumption of oxygen is carried out in accordance with the following formula:
where BOCR - specified rate of biological oxygen consumption;
dDO - change of dissolved oxygen;
dt versus time;
and dDO and dt close to zero.16. The method according to p. 12, characterized in that the said stage of the determination carried out in situ.17. The method according to p. 12, characterized in that the liquid sample is isolated in a chamber for a liquid sample, comprising a diffuser for supply air and/or oxygen in the specified sample fluid and a device for mixing the sample.18. The method according to p. 17, characterized in that it further comprises the steps of aerating the specified liquid sample is specified aerator before saturation of the specified sample liquid by dissolved oxygen to a level above with a stock level of dissolved oxygen in the sample after its release, before the steps of measuring the pH of the specified sample fluid, and periodic mixing of the specified sample of the specified device for mixing during pH measurement specified sample fluid.19. The method according to p. 12, characterized in that the specified microbiological process selected from the group consisting of wastewater treatment, formater CLASS="ptx2">20. The method according to p. 17, characterized in that it further comprises aeration of the specified liquid sample is specified aerator throughout the period of time when the sample is in the cell in which he is selected and dissolved oxygen and pH in the specified pattern are regularly measured with constant stirring specified pattern.21. The method according to p. 12, characterized in that it additionally contains essentially constant aeration of the specified sample fluid.22. The method of regulation of microbiological process in the liquid stream containing the microbial population, providing steps (a) selection of sample fluid from the specified fluid flow, (b) measuring the pH of the specified sample fluid at selected time intervals, (c) determine the speed of establishing pH for the specified sample, (d) determine when the specified speed setting pH changes from a negative value to zero and/or changed to a value of zero for the second time, and (e) regulation in response to changes in said speed (speed) setting the pH, and the specified stage regulation contains f) determine the time of nitrification as the time between the selection about the e value for the second time, g) measuring time hydraulic retention in the specified liquid sample and comparing the specified time nitrification with the specified time hydraulic retention and (h) increasing the rate of supply of fluid in the fluid flow, or a decrease in the rate of aeration of the fluid flow, if the specified time nitrification less than the specified time hydraulic retention, or improvement in the speed of aeration of the fluid flow, if the specified time nitrification more than a specified time hydraulic retention.
FIELD: biotechnology and microbiological industry.
SUBSTANCE: invention concerns governing periodical air-intake biotechnological process carried out in bioreactor. Method comprises measuring oxygen content in effluent gas, working volume of culture medium, concentration of biomass, and concentration of intermediate product of its vital activity. Measured parameters allow specific oxygen consumption rate and velocity of intermediate product concentration change to be determined to enable regulation of feeding air used in aeration, supplying nutritional medium, and agitating culture medium. Moreover, temperature of culture medium, temperature of supplied and withdrawn cooling agent, and consumption of the latter are measured to use these parameters for determining biomass heat release rate and velocity of intermediate product amount change. The two latter parameters enable regulation of feeding air used in aeration and supplying nutritional medium. The following characteristics are thus improved: elevating power by 8.1%, maltase activity by 7.9% and resistance by 7.4%.
EFFECT: enhanced efficiency of governing biotechnological process and improved qualitative characteristics of process.