The method of automatic control of the process of growing microorganisms

 

(57) Abstract:

The invention relates to the microbiological industry, and in particular to methods of automatic control of the process of growing microorganisms. The method of automatic control of the process of growing microorganisms is to regulate the supply of chemical reagent to suppress wild microorganisms in the apparatus for growing microorganisms, depending on the difference between the current concentration of wild organisms and the specified allowable concentration. Regulation of the supply of a chemical reagent is carried out based on the values of the coefficient of efficiency, which is the ratio of the constant destruction of wild organisms to the constant, taking into account the adverse effect of the chemical reagent on the growth of beneficial microorganisms, and coefficient, which is the ratio of the work of the economic factor and the initial concentrations of wild organisms to the initial value of the nutrient substrate. Calculation of coefficient values is performed by the following expressions: a=K/KZ(1) where a is the coefficient of utility; K is a constant extinction of wild organisms; KZis a constant that takes into account pobo is B> - economic factor; y0- initial concentration of wild organisms; S0- the initial concentration of the nutrient substrate. The invention saves the consumption of nutrient substrate by reducing the consumption of wild microorganisms and losses caused by the decomposition of raw materials during the chemical exposure. 1 C.p. f-crystals, 1 Il.

The invention relates to the microbiological industry, and more specifically to methods of automatic control of the process of growing microorganisms.

There is a method of automatic control of the process of growing microorganisms, which consists in suppressing extraneous microflora by filing a chemical reagent in the device (Matveev C. E. fundamentals of aseptic technology pure microbiological preparations. - M: Light and food industry, 1981. - 312 S.; Bocharova, N. N., Kobrin Y. P., Razmanova N. In. Microflora yeast production. - M., 1972, ch. V, sec. 94-102).

However, the known method does not take into account the number of foreign flora and side negative effects of chemicals on the process.

The closest technical solution to the proposed method is a method of automatic control of proselitista extraneous microflora in the apparatus and comparing this number with some valid value (Bezryadin, N. The synthesis of control algorithms in a competitive interactions between populations of microorganisms (for example yeast production): Spec. 05.13.16 - Application of computer technology, mathematical modeling and mathematical methods in scientific research; 05.13.07 - automation of technological processes and productions; author. dis. Kida. technology. Sciences / Voronezh state technology.Acad. - Voronezh, 1997. - S. 8-9).

However, when using this method is not considered negative side effect of chemical reagents on the development of healthy culture, which consists in the decomposition part of the nutrient substrate and deterioration of its quality. In addition, this method does not determine the mode of supply of the chemical reagent, in which the total loss of nutrient substrate (decomposition and consumption of wild organisms) would be minimal.

The technical challenge is to improve the quality control process of growing microorganisms and reducing losses of nutrient substrate using a chemical reagent.

This object is achieved in that in the method of automatic control of the process of growing microorganisms, which consists in baking the dependency on the difference between the current concentration of wild organisms and the specified allowable concentration, what's new is that the control flow of the chemical reagent is carried out based on the values of the coefficient of efficiency, which is the ratio of the constant destruction of wild organisms to the constant, taking into account the adverse effect of the chemical reagent on the growth of beneficial microorganisms, and coefficient, which is the ratio of the work of the economic factor and the initial concentrations of wild organisms to the initial value of the nutrient substrate. Calculation of coefficient values is performed by the following expressions:

a = K/KZ, (1)

where a is the coefficient of usefulness;

K is a constant extinction of wild microorganisms;

KZ- a constant that takes into account the adverse effect of the chemical reagent on the growth of useful microorganisms;

A = YSy0/S0, (2)

where YS- economic factor;

y0- initial concentration of wild microorganisms;

S0- the initial concentration of the nutrient substrate.

The technical result of the invention consists in minimizing the total losses of nutrient substrate (decomposition and consumption of wild microorganisms) by controlling the supply of chemical Rea the system of automatic control, realizing the proposed method.

The system contains: sensors, respectively, the concentration of wild organisms in the apparatus 1 and the nutrient substrate 2; the unit allowable number of wild microorganisms 3; unit 4 definitions of constants extinction of wild organisms and constants, taking into account the adverse effect of the chemical reagent on the growth of useful microorganisms; block 5 determination of the coefficient of efficiency and coefficient, which is the ratio of the work of the economic factor and the initial concentrations of wild organisms to the initial value of the nutrient substrate; a logical unit 6 test conditions minimum total losses of nutrient substrate (decomposition and consumption of wild microorganisms); unit 7 determination of the difference between the current concentration of wild organisms and the specified allowable concentration; control device 8.

The system works as follows.

The signals proportional to current concentration values of wild microorganisms and a nutrient substrate, respectively, from sensors 1 and 2, proceed to unit 4 definitions of constants extinction of wild organisms and constants, the correction of these constants on the current values of the concentrations of the wild microorganisms and a nutrient substrate. From the output of block 4 is supplied to the input unit 5 definition of the coefficient of efficiency and coefficient, which is the ratio of the work of the economic factor and the initial concentrations of wild organisms to the initial value of the nutrient substrate. To the second input unit 5 receives a signal that is proportional to the initial concentration of wild microorganisms from the sensor 1, the third input signal, proportional to the initial concentration of nutrient substrate from the sensor 2. The economic value of the coefficient is set in advance in the block 5.

The output unit 5 is fed to the input of logical unit 6 test conditions minimum total losses of nutrient substrate (decomposition and consumption of wild microorganisms), which verifies the inequality

O < A A. (3)

If the condition is true, then the output signal of the logic unit 6 is fed to the input of block 7 determination of the difference between the current concentration of wild organisms and the specified allowable concentration. Therefore, two other input unit 7 receives signals proportional to the current value of the concentration of wild organisms in the apparatus and given say who drove to the input control device 8, generating in accordance with a value obtained difference by a control action, which is supplied to the actuator with the regulator, located on the supply line of the chemical reagent in the device.

If condition (3) is not satisfied, then the optimal mode of the process of growing microorganisms will be a constant supply of chemical reagent in the device. Therefore, from the second output of the logic unit 6 test conditions minimum total losses of nutrient substrate (decomposition and consumption of wild organisms) signal directly to the control unit 8, which provides a constant flow of chemical into the system.

The invention saves the consumption of nutrient substrate by reducing the consumption of wild microorganisms and losses caused by the decomposition of raw materials during the chemical exposure.

1. The method of automatic control of the process of growing microorganisms, providing for the regulation of the supply of the chemical reagent to suppress wild microorganisms during cultivation of microorganisms, depending on the difference between the current concentration of wild organisms and given dopustite extinction of wild organisms and the constant, taking into account the adverse effect of the chemical reagent on the growth of beneficial microorganisms, calculate the coefficient of efficiency and the coefficient defined depending on the relationship of the work of the economic factor and the initial concentrations of wild organisms to the initial value of the nutrient substrate, and the supply of chemical reagent to suppress wild microorganisms is performed with the consideration of the calculated coefficients.

2. The method according to p. 1, characterized in that the coefficient of efficiency is determined by the formula

a = K / KZ,

where a is the coefficient of usefulness;

It is a constant death wild microorganisms;

TOZ- a constant that takes into account the adverse effect of the chemical reagent on the growth of beneficial microorganisms.

 

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