(G01N33/543)

Immunochromatographic test system for pathogenic helicobacter pylori strains detection // 2642588
FIELD: biotechnology.SUBSTANCE: immunochromatographic test system for detection of pathogenic H. pylori strains based on cagA protein is a multimembrane composite consisting of a nitrocellulose membrane with a membrane impregnated with a monoclonal antibody conjugate, a HP-387 clone at a concentration of 20 mcg⋅cm-3 with nano-particles of colloidal gold with an average diameter of 30 nm, a sample membrane and an adsorbing membrane. Monoclonal antibodies, HP-1811 clone at a concentration of 12 mcg⋅cm-3 are applied to the analytical membrane in the test zone, and in the control zone - goat anti-species antibodies against mouse Ig are applied at a concentration of 10 mcg⋅cm-3.EFFECT: invention provides determination of the said protein in various biological materials.1 dwg, 1 tbl, 3 ex

ethods for selective quantitative assessment of a-beta aggregates // 2642314
FIELD: biotechnology.SUBSTANCE: method for quantitative assessment and characterisation of A-beta aggregates is described, comprising the following steps: a) entrainment molecules immobilisation on a substrate, b) application of a test sample and an internal standard to the substrate, c) addition of probes labeled for detection to label A-beta aggregates through specific binding to them, and d) determination of the number and size of labeled A-beta aggregates with spatial resolution in each case compared to the corresponding background, at that step b) can be carried out prior to step c). Also a kit for implementation of the described method, comprising a standard and one or more of the following: a glass substrate coated with a hydrophobic substance; an entrainment molecule; a probe; a substrate with the entrainment molecule; solutions; and a buffer, is presented. A method for determination of the efficacy of active substances and/or therapies for Alzheimer's disease treatment is provided, characterized by a) entrainment molecules immobilization on the substrate, b) test sample and internal standard application to the substrate, c) addition of probes labeled for detection, that label A-beta aggregates through specific binding to them, and d) determination of the number and size of labeled A-beta aggregates with spatial resolution in each case compared to the corresponding background. Step b) can be performed prior to step c), e) the results obtained on samples that are samples of a body fluid taken before or at various times after active substances administration and/or therapy are compared with the results obtained for control, which was not treated with the active substance and/or subjected to therapy, and based on the results obtained, active substances and/or therapies are selected, after which a reduction in the amount of A-beta aggregates was observed.EFFECT: invention expands the arsenal of means intended for definition of efficiency of Alzheimer's disease treatment.43 cl, 7 dwg, 3 ex
Elisa test system for segological diagnostics of cattle nodulous dermatitis - dermatitis nodularis bovum // 2640192
FIELD: biotechnology.SUBSTANCE: test system for serodiagnosis of cattle nodulous dermatitis contains a cultural positive antigen of cattle nodulous dermatitis virus of E-95 strain, cultural negative normal antigen, capturing antibodies - specific hyperimmune polyclonal rabbit serum, detecting antibodies -specific hyperimmune polyclonal guinea pig serum antispecies conjugate -immunoglobulins against guinea pig IgG, conjugated with horseradish peroxidase, 0.05M carbonate-bicarbonate buffer, tris-buffer solution, washing buffer solution, blocking buffer solution, buffer for sample and conjugate dilution, ABTS solution and staining stopping solution - 1% solution of SDS.EFFECT: invention provides creation of a modern, highly specific test system for detection of cattle nodulous dermatitis in virus antigen by solid indirect sandwich ELISA, due to application of E-95 strain when receiving specific reaction components for ELISA.2 cl, 6 dwg, 4 tbl, 6 ex

ethod for multispecific binding agent detection // 2636822
FIELD: medicine.SUBSTANCE: invention relates to a method for determination of the total volume of therapeutic multispecific antibody in a serum or plasma sample using a sandwich-type immunoassay, encompassing a stage of determination of the amount of complex formed between I) an anti-idiotypic antibody which specifically binds to the first binding specificity of the therapeutic multispecific antibody and II) therapeutic multispecific antibody by complex incubation with the anti-idiotypic antibody which specifically binds to the second binding specificity of therapeutic multispecific antibody, different from the first binding specificity, and thus the volume of therapeutic multispecific antibody is determined in the sample.EFFECT: improved immunoassay for determination of the multispecific antibody volume in a sample.2 cl, 4 dwg, 5 tbl, 3 ex

ethod for determination of free binding partner of multispecific binder // 2633488
FIELD: medicine.SUBSTANCE: method for in vitro determination of presence and/or concentration of the binding partner of the multispecific binder in which the binding partner can be specifically linked to the first binding region of the multispecific binder comprising the steps of: incubation of a sample comprising a binding partner and a multispecific binder with a monospecific binder that specifically binds to the second multispecific binding region of the multispecific binder different from the first binding region. Elimination from the sample of the monospecific binder-multispecific binder complex prior to determination of the presence or concentration of the free binding partner. And determination of binding partner concentration in the sample, from which the multispecific binder is eliminated. The group of inventions also relates to the use of an anti-idiotype antibody that specifically binds to the second binding region of the multispecific antibody in the above method.EFFECT: use of this group of inventions allows to eliminate the binding partner that is specifically associated with the multispecific binder from the sample, prior to determination of the presence or concentration of the free binding partner.35 cl, 7 dwg

ethod for measurement of antibodies to wt1 // 2633068
FIELD: medicine.SUBSTANCE: method comprises an immunoassay using a polypeptide with antigenicity against an antibody to WT1 selected from i) a polypeptide comprising a sequence with amino acids at 294-449 positions of the SEQ ID NO: 1 sequence and ii) a polypeptide comprising an amino acid sequence with deletion, substitution or addition of one to several amino acids in the amino acid sequence constituting the polypeptide i). The group of inventions also relates to a method for WT1-related disease diagnostics comprising measurement of an antibody against WT1 in a sample and diagnosing of the presence of a WT1-related disease if the concentration of an antibody against WT1 is significantly increased compared to the anti-WT1 antibody concentration in a healthy individual; a method for prediction of the patient's response to therapy with WT1 vaccine; a reagent for measurement of the antibody to WT1 in the sample.EFFECT: high accuracy and sensitivity in determination of a group of cancer patients.10 cl, 5 ex, 12 dwg, 1 tbl
ethod for bubble adsorption reduction // 2629811
FIELD: medicine.SUBSTANCE: to reduce the adsorption of bubbles on the side surface of the plastic cell in the immunoassay, the method involves an antigen-antibody reaction in a dry plastic cell, where the method comprises surfactant addition to the reaction system before or simultaneously with the onset of the said antigen-antibody reaction. The said surfactant is a poly(oxyethylene) ester of sorbitol and fatty acid or a polyoxyethylene tribenzyl phenyl ether. The group of inventions also relates to a reagent for immunoassay.EFFECT: use of this group of inventions allows to effectively prevent the adsorption of bubbles on the spectrophotometric surface of a dry plastic cell, as a result of which the measurement value is stabilized and the measurement accuracy in the immunoassay is increased by means of an automated analyzer.7 cl, 6 tbl
ethod for early diagnostics of cervical intraepithelial neoplasias (cin) based on metabolic markers study // 2629632
FIELD: medicine.SUBSTANCE: invention is intended for early diagnostics of cervical intraepithelial neoplasias (CIN). Cervix pathology is identified. The level of predictor markers expression is determined by means of a Western blot analysis with chemiluminescence detection and real-time PCR. At expression levels of HIF1alpha 0.029, GLUT1 782.9±156.5, IGFBP-3 0.038±0.0076, HK2 36.4, VDAC1 70, CIN1 (LSIL) is diagnosed. At expression levels of HIF1alpha 0.03, GLUT1 1154.2±88.7, IGFBP-3 0.02±0.005, HK2 44.9, VDAC1 87, CIN2-3 (HSIL) is diagnosed.EFFECT: effective diagnosis of cervical intraepithelial neoplasias at earlier stages.5 dwg, 1 tbl, 3 ex

Calibrational reagent and method // 2629310
FIELD: physics.SUBSTANCE: group of inventions refers to the technique of the calibration of the composite analysis, including: adding a calibration the reagent, consisting of at least two different binding molecule, where each molecule has the ability to link to a specific agent capture and the ability to communicate with the detecting molecule and where at least two of the linking molecules have different specificity and are present in different concentrations, addition of detecting molecules, detection of detection-related molecules, the establishment of the calibration curve, including a number of calibration points or intervals. A group of inventions refers to a set of reagents and analytical system.EFFECT: use of a group of inventions makes it possible to improve the accuracy of results due to the systemic variability of systematic analyzes over time.20 cl, 7 dwg, 4 tbl, 3 ex

ethod for colorectal cancer diagnosis/screening based on simultaneous quantification of protein nature tumour markers, glycans antibodies, g, a and m immunoglobulins in human blood on biological microchip // 2625018
FIELD: medicine.SUBSTANCE: method involves the simultaneous quantification of protein nature tumour markers, glycan antibodies, G, A and M immunoglobulins in human blood on a biological microchip. For this purpose, the biochip contains hydrogel elements with immobilized antibodies to protein oncomarkers, hydrogel elements with immobilized glycans, hydrogel elements with immobilized antibodies against human immunoglobulins of G, A, M classes. The change in the level of the analyzed markers in comparison with the level in the serum of healthy donors indicates colorectal cancer. The group of inventions also refers to a biological microchip, which is an array of three-dimensional hydrogel elements.EFFECT: application of this invention allows colorectal cancer diagnosis, screening, simultaneous quantification of protein markers, antibodies to glycans, G, A and M immunoglobulins in human blood on a biological microchip that allows successive reactions of various types.8 cl, 5 ex, 5 tbl, 7 dwg
ethod for detecting binding partner for multispecific binding agent // 2620563
FIELD: chemistry.SUBSTANCE: it provides a method for detecting free antigen biospecific antibodies in the sample, wherein the antigen to be determined can be specifically linked to the first binding site biospecific antibodies comprising the sample incubation step comprising free antigen and biospecific antibody to anti-idiotypic antibody which specifically binds to second binding bispecific antibody determinant different from the first binding determinant, wherein the anti-idiotype antibody bound to a solid phase.EFFECT: group of inventions can effectively determine the presence, concentration of free antigen biospecific antibodies in the sample.9 cl, 13 dwg, 9 ex

ethod for specific substance detection in milk // 2617400
FIELD: veterinary medicine.SUBSTANCE: group inventions concerns a immunochromatographic method for detection of a specific substance contained in milk, which includes milk processing with lytic enzyme or surfactant; milk contact with the test strip comprising the first portion containing the first labeled antibody, the second portion located below the first portion, in where the second antibody is immobilized, and the third portion located above the first or the second portion and containing voids to ensure removal milk fat globules; milk flowing through the third portion of the milk to remove some of the milk fat globules of milk; and milk flowing through the second portion or the subsequent portion below to obtain the detectable label signal in the second portion or the subsequent portion below. The group of inventions also relates to immunoassay devices for specific substance detection in milk; kit for specific substance detection in milk.EFFECT: ability to detect certain specific substances at dairy farms without the need for an additional device for milk fat globules removal.14 cl, 7 ex, dwg 7, 8 tbl
ethod for fat embolism syndrome diagnosis in case of lower limb bone fractures // 2611363
FIELD: medicine.SUBSTANCE: invention is intended for fat embolism syndrome (FES) diagnosis in case of lower limbs fractures. On the first day of the post-traumatic period, patient's venous blood is collected subsequently centrifuged. The concentration of surfactant protein D in the obtained serum is determined using the immunoenzyme technique. FES is diagnosed if the protein standard of 200 ng/ml is exceeded.EFFECT: early detection of fat embolism syndrome.1 tbl, 2 ex

Device used for detecting of binding affinity // 2609184
FIELD: biotechnology.SUBSTANCE: invention relates to devices used for detecting binding affinity and can be used in biosensors. Device contains planar waveguide (2), arranged on substrate (3), and optical isolation (4) for output of coherent light (1) of preset wave length into planar waveguide. Coherent light passes through planar waveguide (2), and evanescent field (6) spreads along outer surface (5) of planar waveguide. Outer surface (5) of planar waveguide contains binding sites (7), able to bind target samples (8) on it with binding sites (7) so, that light of evanescent field (6) is scattered by target samples (8), associated with binding sites (7). Binding sites (7) are arranged along multiple specified lines (9), arranged so, that scattered light structurally interferes in specified detection position with difference of optical path length, being whole multiple of given wavelength.EFFECT: technical result is high efficiency of detecting, universality of design.24 cl, 21 dwg

Electrochemical biosensor for direct recording of myoglobin based on carbon nanotubes and molecular imprinted polymer based on o-phenylenediamine and preparation method thereof // 2604688
FIELD: chemistry.SUBSTANCE: group of inventions relates to analytical chemistry, electrochemistry and medical diagnosis and can be used for diagnosing early stages of myocardial infarction. Electrochemical biosensor for direct recording myoglobin in aqueous buffer solutions by measurement of peak height of electroreduction myoglobin by square-wave voltammetry is a graphite electrode, modified with carbon nanotubes and analogue of anti-myoglobin antibodies, which is an o-phenylenediamine polymer which is molecular-imprinted for myoglobin. Group of inventions also relates to a method of producing said biosensor, including modification of surface of graphite electrode with a suspension of carbon nanotubes, subsequent modification of obtained electrode by electropolymerisation of o-phenylenediamine in presence of myoglobin, performed on surface of nanotube-modified electrode, and incubation of obtained electrode in a solution containing ethanol and 0.25 M NaOH at ratio 2:1. Suspension of carbon nanotubes is produced by ultrasonic disintegration of carbon nanotubes in an organic solvent.EFFECT: group of inventions provides high sensitivity of biosensor.2 cl, 2 dwg, 2 ex

Improved coded microcarriers, test systems and analysis methods using them // 2599304
FIELD: micro-appliances.SUBSTANCE: invention relates to coded microcarrier and, in particular, to microcarrier containing spatial element; to test system and method for conducting chemical and/or biological analysis. Coded microcarrier (2) comprises readable code for its identification. Microcarrier includes body (3) having at least one detection surface (6) for detection of chemical and/or biological reaction. Microcarrier contains therein at least one spatial element (9) that projects from body (3) and have shape, which, when coded microcarrier (2) lies on plane (10) with detection surface (6) facing to plane (10), can provide for clearance (11) between plane (10) and detection surface (6). Contact surface (14) therein, contacting with plane (10) is located at distance from detection surface (6), and maximum distance (d) between detection surface (6) and plane (10) is more than 5 % of maximum height (H) of coded microcarrier (2), preferably more than 10 %. Test system (100) comprises multiple coded microcarriers and analyzing device (101) having at least one microfluidic channel (102), having shape allowing to place multiple coded microcarriers (2). Microfluidic channel (102) has at least one wall for observation (106), through which analytical control is performed. Microfluidic channel (102) and spatial elements (9) of each microcarrier (2) have such shape, that can ensure clearance (10) between detection surface (6) and wall for observation (106) in order to allow fluid circulation in said gap (10). Disclosed method comprises using at least one coded microcarrier (2). Control over chemical and/or biological reaction therein is carried out on detection surface (6) of coded microcarrier (2).EFFECT: providing fast quantitative analysis of analyzed drenching substance.13 cl, 9 dwg

ethod of functionalising surfaces for detecting analytes // 2597768
FIELD: chemistry; instrumentation.SUBSTANCE: group of inventions relates to diagnostics, specifically to a device for detecting analytes, including plastic substrate, partially or completely directly coated with binding polymers, fixed on substrate noncovalently, wherein said binding polymers contain polysaccharide frame equipped with: aromatic groups of form -X-CONH-Z, groups of carboxylic acid of form -X-COOH and reactive groups F, having form -X-CONH-Z′, where X denotes a straight or branched, substituted or unsubstituted alkyl chain containing from 1 to 6 carbon atoms, Z denotes an aryl function, Z′ denotes a group which is capable of binding with other molecule, as well as methods for production of said device, and also to binding polymer and method of its production.EFFECT: group of inventions enables to obtain a device for detecting different analytes.20 cl, 13 dwg, 34 ex

Analysis method and device using magnetic particles // 2595843
FIELD: medicine.SUBSTANCE: group of inventions relates to diagnostics. Method of detecting an analyte in sample involves application of device for lateral flow analysis (1) containing zone of addition of sample (2), reaction zone (4) and absorption zone (5), wherein said zone form flow path for said sample. Above device also comprises first and second capture molecules able to bind to analyte, wherein first grip molecule carries first mark, which is not magnetic mark, and second grip molecule is attached to magnetic particle. At least part of the flow path contains micro posts representing ledges, in fact, vertical relative to surface and having height, diameter and distance from each other to allow creation of lateral sample flow in said flow path. Method also involves use of movable magnetic force for movement of said second capture molecules with attached magnetic particle. Group of inventions also relates to lateral flow analysis device and devices for reading analysis result on said device.EFFECT: group of inventions allows simplifying movement of magnetic particles and improves flow control.45 cl, 5 dwg, 1 tbl, 2 ex
iniature magnetic flow cytometry // 2582391
FIELD: chemistry.SUBSTANCE: group of inventions relates to field of magnetic detection of cells, namely to magnetic flow cytometry. Device for magnetic flow cytometry includes magnetoresistive sensor, flow chamber, intended for passage of cell suspension flow, and concentration section for orientation and concentration of magnetically labelled cell sample. Concentration section is made in form of meander. Method for manufacturing device for magnetic flow cytometry and method for magnetic detection of cells with application of said device are also disclosed.EFFECT: device for magnetic flow cytometry is more compact due to application of concentration section, made in form of meander.14 cl, 5 dwg

ethod of making microbiosensor for determining glucose or lactate // 2580288
FIELD: biotechnology.SUBSTANCE: invention relates to biological sensors and can be used for analysing biological samples containing glucose or lactate. Method of making a microbiosensor based on iron hexacyanoferrate consists in that on a working electrode, coaxial with a comparison electrode, is applied ferrocyanide iron, and on top it is applied enzyme-oxidase, immobilised in a matrix based on a perfluorosulphonylated polymer or gamma-aminopropylsiloxane.EFFECT: achieving reliability and reproducibility of fixing enzyme on surface of electrocatalyst, while maintaining most of its activity; and interfacing electrode and enzymatic reactions.3 cl, 6 dwg, 2 tbl, 2 ex

ethod of coating nanoparticles // 2578439
FIELD: nanotechnology.SUBSTANCE: invention relates to the method of coating nanoparticles with minimum amount of binding agent, compositions of nanoparticles and a kit for detection by the method of localised surface plasmon resonance. Method includes the stage of determining the minimum amount of binding agent required to obtain stable nanoparticles in the presence of said non-ionic, cationic and/or zwitterionic detergent, and the stage of mixing the solution containing nanoparticles with the solution containing said binding agent. EFFECT: said solution containing nanoparticles and/or the said solution containing the binding agent includes non-ionic, cationic and/or zwitterionic detergent and provides an electrostatic interaction between nanoparticles and binding agent.15 cl, 6 dwg

Apparatus for detecting antigens and use thereof // 2574991
FIELD: chemistry.SUBSTANCE: group of inventions relates to detection of food pathogenic contaminants by determining positive or negative reaction to a target molecule. An apparatus for detecting a target molecule comprises a housing and a membrane detection system. The housing has an inlet opening for feeding a sample into the membrane detection system, a clamp, a movable lock in contact with the clamp, a connector in contact with the lock, and a sliding button whose movement moves the movable lock. The membrane detection system comprises a spacer for conjugation, a test membrane and an absorbent element, wherein parts of the membrane detection system are parallel to each other. The lock is in contact with the absorbent element and can apply pressure perpendicular to the membrane detection system. The invention also discloses a system and a kit comprising a detector and a method of detecting a target molecule using the disclosed detector.EFFECT: detector improves sensitivity and rate of detection.25 cl, 10 dwg, 1 ex

ethod of binding mycobacteria // 2573921
FIELD: biotechnology.SUBSTANCE: method of binding Mycobacteria and/or fragments of Mycobacteria is provided, which present in an aqueous liquid with the solid surface. The said liquid is added to a sufficient amount of a water soluble polymer in the presence of a solid surface to displace Mycobacteria and/or their fragments from the said liquid onto the solid surface. The surface may be provided in the form of a microsphere. The water-soluble polymer can be selected as, for example, dextran, polyethylene glycol, polyvinylpyrrolidone.EFFECT: invention enables to implement binding to a solid surface of Mycobacteria and their fragments that are not precipitated under conditions of centrifugation.19 cl, 11 ex

Immunochromatography devices, methods and kits // 2568875
FIELD: chemistry.SUBSTANCE: group of inventions relates to devices and methods for analysis using specific binding and is intended for determining the presence or amount of an analyte in a sample. The immunochromatographic device comprises a membrane carrying a capture antibody bound thereto in a test zone, and a structure having conjugates, fluidly coupled to the membrane at a proximal end. The structure comprises a detector antibody having a reporter moiety conjugated therewith. Each of said capture and detector antibodies is a monoclonal antibody produced by hybridoma cells deposited under access number DSM ACC3092 (monoclonal antibody LG96) or DSM ACC3093 (monoclonal antibody MG97). The analyte is a Z-AAT protein, present in the sample from a PiZ gene carrier.EFFECT: use of the group of inventions improves the efficiency of determining presence of an analyte, particularly a Z-AAT protein.25 cl, 33 dwg, 1 tbl, 5 ex

ethod of treatment of blood sample at immunological analysis of allergen-specific and total immunoglobulins e // 2568242
FIELD: biotechnologies.SUBSTANCE: before the immunoanalysis the immunoglobulins G are removed from the sample, in particular, by interaction of the sample with the sorbent which is specifically binds immunoglobulins G then the immunological analysis of allergen-specific and total immunoglobulins E is conducted using the allergens or mixes of allergens and antibodies against the immunoglobulins E immobilised in the elements of the biological microchip which is the array of three-dimensional hydrogel elements containing elements with covalently immobilised allergens and antibodies against the immunoglobulin E, while the array elements are microdrops obtained by the polymerisation immobilisation method.EFFECT: decrease of non-specific signals, increase of the ratio signal-background, increase of reproducibility of the measured specific signals and improvement of sensitivity and reproducibility at analysing for determination of total and allergen - specific immunoglobulins.3 cl, 2 dwg, 2 tbl, 2 ex

Single target immunoassay // 2566714
FIELD: medicine.SUBSTANCE: group of inventions refers to medicine, namely to laboratory diagnostics, and is applicable to visualise a single specific target unit in a sample wherein the above target is immobilised. That is ensured by incubating the sample containing a population of specific target units with a binding agent containing an enzyme; the binding agent is able to bind directly to the single specific target unit; the incubation process is followed by forming a single discrete target site with a fraction target sub-population, wherein each single discrete target site represents a complex of one specific unit of the above fraction sub-population and the binding agent. The sample is incubated in aqueous solution containing a peroxide compound in an amount making from 0.1 mM to 5 mM, a first enzymatic substrate bound to the single target sites, and a second enzymatic substrate, wherein the first substrate is a water-soluble electron-rich compound, to form a water-insoluble polymer product. The second substrate represents a conjugated molecule with a detectable label, wherein the detectable label is specified in a group consisting of fluorescent, luminescent, radioactive or chromogenic substances or members of specific binding pairs to form discrete deposits of the second substrate. Visualising the single target sites.EFFECT: a method for quantitative estimation of the immobilised target in the sample, a diagnostic technique, a method for prediction of a risk of disease development and assessment of the efficacy of therapeutic treatment in the patient are also presented.63 cl, 3 dwg, 5 tbl, 10 ex

ulti-layered analysis with horizontal flow with press for samples // 2564911
FIELD: medicine.SUBSTANCE: group of inventions relates to the field of medicine and can be used for diagnostics on a treatment site. A device with a horizontal flow for the detection of an analysed substance in a sample includes a press for samples, which contains an insert, a sampler, a chromatographic test-strip with the horizontal flow, including a zone of the sample application and a test zone; a conjugate, including the first binding partner for the analysed substance and a mark, the second binding partner for the analysed substance and the first control binding partner, placed on the insert of the press for samples. The test-strip additionally contains a control zone, which contains the second control binding partner, with the first control binding partner being the binding partner for the second control binding partner. The press for samples, the sampler and the test-strip form a vertical pile for the application of the sample on the said test-strip by pressing, with the sampler being placed in the vertical pile between the press for samples and the test-strip. The group of inventions also relates to versions of the said device with the horizontal flow, the press for samples for application in the said device and the method of applying the sample on the chromatographic test-strip with the application of the said press for samples.EFFECT: group of inventions provides the sensitive and fast method of detecting analysed substances in the sample.20 cl, 21 dwg
ethod for increasing diagnostic effectiveness of immunochromatographic systems of pathogen detection // 2557936
FIELD: medicine.SUBSTANCE: method is based on contacting a membrane test strip with an analysed fluid sample and initiating thereby a motion along the test strip membranes of reagents being parts of the sample or coating the membrane, and forming the immune complexes to be detected in the course of reactions in the membrane pores or on the surface thereof. A distinguishing feature of the presented method for antigen detection is that the test strip is coated additionally within the test sample contact area with a certain amount of specific antibodies, which react to the detected antigen expected to be found in the sample, when a fluid front moves and block a certain number of binding sites. The number of the coating free antibodies is specified so that the low content thereof in the analysed sample being of no diagnostic importance ensures blocking the binding sites completely that prevents the antigen from binding in the analysed area of the test strip and from developing a destructive staining in the analysed area.EFFECT: presented approach enables reliable diagnosing based on the detection results of the antigens of gastrointestinal disorders, avoiding the achievement of positive test results for the low-antigen samples, which testifies to no development of the disease in an individual.1 tbl, 2 ex

Target sensor // 2553626
FIELD: medicine.SUBSTANCE: group of inventions refers to medicine and can be used for laboratory diagnostics. A target sensor comprises a light source, a light receiver, a sample assembly for connecting the target placed between the light source and light receiver, a light selection unit making the light of pre-set wave length be received by the light receiver, and a detector configured to generate an electric signal an intensity of which reflects an amount of light received by the light receiver. The sample assembly comprises a carrier, a material on the carrier and forming a film with a structure formed by annealing, and a probe on the material and configured to connect to the target. The sample assembly is configured to as to be found on the passed light path emitted by the light source. The group of inventions also refers to a version of the above sensor, wherein the light selection unit is replaced by the light source, which emits the light of pre-set wave length.EFFECT: group of inventions provides the high-sensitivity detection of the target molecule over a short period of time.19 cl, 8 dwg, 3 ex

Target detection sensor // 2553371
FIELD: measurement equipment.SUBSTANCE: group of inventions relates to medicine and can be used for laboratory diagnostics. A target detection sensor includes a container, a probe located in the container and configured for connection to the target, a circulation device for circulation of substances in the container, a light source, a light receiver, a light selection unit and a detector configured for generation of an electrical signal, the value of which reflects the amount of light, which is received with the light receiver. The container is arranged between the light source and the light receiver; with that, the probe and the path passed by light emitted with the light source are located in different areas inside the container. The probe can be located in the upper part of the container, and the path that is passed by light is located in the lower part of the container, or the probe can be located in the first area of the upper part of the container and the path that is passed by the light is located in the second area different from the first area in the upper part of the container. The group of inventions also relates to a version of the specified sensor, in which instead of the light selection unit the sensor includes a light source that emits light of the specified wave length.EFFECT: group of inventions provides for detection of a target molecule during a short period of time.23 cl, 8 dwg, 6 ex

Sensor to detect target // 2553369
FIELD: measurement equipment.SUBSTANCE: group of invention relates to the field of immunological analysis and is designed to detect a target by interaction of a biomolecule with a target molecule. A target detection sensor includes a source and a receiver of light, a detector, a device for target binding. At the same time the target binding device comprises a container with a substrate, a material containing metal, arranged on the substrate, representing a film or an array of rods, configured to scatter light, a probe located on the material and configured for binding with the target, and also configured to scatter light emitted by the source of light as the target binds with the probe, and a drainage pump. At the same time the target binding device is placed between the source of light and is configured so that the probe is located on the way of light emitted by the source of light. Also the method of production and usage of the sensor with such design is disclosed.EFFECT: sensor provides for detection of a target molecule with high approximation during a relatively short period of time.19 cl, 6 dwg, 3 ex

Sensor for desired target identification // 2546020
FIELD: medicine.SUBSTANCE: invention relates to the field of immunoassays and is intended for the identification of a desired target by the interaction of a biomolecule with a target molecule. A sensor for the identification of the desired target includes a device for binding the desired target and a discharge device for the discharge of liquid from the device. The device for the desired target binding contains a container with a substrate, a metal-containing material, located on the substrate, representing a film or an array of rods, configured for light dispersion, and a probe, located on the material and configured for binding with the desired target, as well as configured for the dispersion of the light, emitted by a light source when the desired target is bound with the probe. The discharge device contains the first hole for connection with a pump, the second hole for connection with the device output, with the holes being located on opposite walls of the chamber and displaced with respect to each other. The discharge device also contains a blocking device, which extends from the wall, which contains the second hole, to the opposite wall.EFFECT: sensor provides the identification of the target molecule within a relatively short time period.18 cl, 12 dwg, 6 ex

Biosensor // 2546018
FIELD: medicine.SUBSTANCE: group of inventions relates to the field of medicine and can be applied for the detection of a target molecule in a biological sample. A sensor for detecting the target of interest contains: the first electrode; the first molecule with electronic conductivity, configured for binding with the first electrode; the first probe, conjugated with the second molecule with electronic conductivity; the second electrode; the third molecule with electronic conductivity, configured for binding with the second electrode; the second probe, conjugated with the third molecule with electronic conductivity. The first probe is configured for binding with the target of interest in a solution, the second probe does not bind with the target, and the first and second molecules with electronic conductivity differ from each other. The group of inventions also relates to a method of detecting the target of interest, which includes contact of the said sensor with the sample, generation of the first signal when the target binds with the first probe and the second signal without binding of the target with the second probe. The target is detected by the comparison of the first and second signal value with a specified value, indicating the presence of the target in the sample.EFFECT: group of inventions provides an increased accuracy of detecting the target molecule in a biological sample.42 cl, 12 dwg, 3 ex

ethod of intraoperative visualisation of pathological foci // 2544094
FIELD: medicine.SUBSTANCE: invention relates to medicine, namely to surgery and diagnostic research methods, in particular to intraoperative visualisation. The targeted delivery of conjugates of nano-sized anti-Stokes phosphori (NAP) with molecules, selectively binding with a target biostructure, subjected to visualisation is carried out. Irradiation of a pathological focus by infra-red radiation in the range of 975-980 nm is carried out. Intraoperative visualisation of the luminescence of the surface and subsurface pathological foci is performed in the blue spectral range by the naked eye. Deep optic probing by means of an optic probe is performed to register the pathological foci, located at the depth, mainly, in the infra-red spectral range.EFFECT: method provides high sensitivity of the differentiation of the pathological foci from normal tissues, high resolution of visualisation, makes it possible to differentiate the surface and subsurface pathological foci by the naked eye, and the pathological foci, located at the depth, by means of the optic probe.4 cl, 3 ex, 7 dwg

Target-modified endopeptidase activity immunoassays // 2543650
FIELD: medicine.SUBSTANCE: invention refers to medicine and aims at detecting target-modified endopeptidase activity. A method according to the invention involves the stage of processing a cell of a stable cell line with a sample containing target-modified endopeptidase, recovering SNAP-25 component containing SNAP-25197 splitting product a carboxyl terminal of which correlates with P1 residue of a split bond in a BoNT/A-toxin restriction site, from the processed cell, contacting the SNAP-25 component and anti-SNAP-25 antibody immobilised on a solid-phase substrate, and detecting the presence of the antibody-antigen complex including the anti-SNAP-25 antibody and SNAP-25197 splitting product. Detecting the SNAP-25197 splitting product by the above antibody-antigen complex is indicative if the target-modified endopeptidase is active.EFFECT: invention provides detecting the target-modified endopeptidase activity effectively.6 cl, 9 dwg, 33 tbl, 17 ex

ethod of analysis and analytic device // 2538652
FIELD: chemistry.SUBSTANCE: group of inventions relates to an analysis of liquid samples. Claimed is a method of analysis of at least two analytes in a liquid sample; the said method contains the following stages: supply of a substrate, where at least two different types of binding molecules are immobilised on the substrate and where each type of the binding molecules possesses specific affinity to analyte, b) bringing the sample in contact with the said binding molecules, c) realisation of the contact between the binding molecules and a labelled identifiable molecule with specific affinity to analyte for at least one analyte to be analysed, and realisation of the contact between the binding molecules and a labelled version of analyte for at least one other analyte to be analysed, and d) measurement of an identified signal from the labelled identifiable molecule and labelled analyte on the substrate, where the concentration of the labelled analyte is selected in accordance with the analyte concentration in the sample. An analytic device is also claimed.EFFECT: simplification and increase of the analysis reliability is achieved.14 cl, 4 dwg

ethod and apparatus for antigen retrieval process // 2538022
FIELD: chemistry.SUBSTANCE: group of inventions relates to a method for retrieval of an antigen in a formaldehyde-fixed tissue sample, and to a kit used in said method. The method includes incubating the formaldehyde-fixed tissue sample in a first antigen retrieval solution at a temperature higher than 90°C; transferring the formaldehyde-fixed tissue sample to a second antigen retrieval solution; and incubating the formaldehyde-fixed tissue sample in the second antigen retrieval solution at a temperature higher than 90°C. The first antigen retrieval solution comprises a buffer solution having a pH range of between about 5 and about 7 and the second antigen retrieval solution comprises a buffer solution having a pH range of between about 7.5 and about 11. Alternatively, the first antigen retrieval solution comprises a buffer solution having a pH range of between about 7.5 and about 11 and the second antigen retrieval solution comprises a buffer solution having a pH range of between about 5 and about 7. The kit comprises a first antigen retrieval solution which retrieves at least a portion of unretrieved antigens in the sample, and a second antigen retrieval solution which retrieves at least some of another portion of unretrieved antigens in the sample. The first solution comprises citric acid, potassium dihydrogen phosphate, boric acid, diethyl barbituric acid, piperazine-N,N′-bis(2-ethanesulphonic acid), dimethylarsinic acid, 2-(N-morpholino)ethanesulphonic acid, or a combination thereof, and the second solution comprises tris(hydroxymethyl)methylamine (TRIS), 2-(N-morpholino)ethanesulphonic acid (TAPS), N,N′-bis(2-hydroxyethyl)glycine (Bicine), N-tris(hydroxymethyl)methylglycine (Tricine), 4-2-hydroxyethyl-1-piperazineethanesulphonic acid (HEPES), 2-{[tris(hydroxymethyl)methyl]amino}ethanesulphonic acid (TES), or a combination thereof.EFFECT: said steps of using the first and second antigen retrieval solutions improve antigen retrieval in tissue compared to use of the first solution without the second solution and vice versa.17 cl, 5 dwg
Device for identification of nucleotide sequences // 2537267
FIELD: chemistry.SUBSTANCE: invention relates to the field of molecular biology and biochemistry. A device consists of a light source, the radiation of which is directed on a transparent substrate with oligonucleotides immobilised on its surface and a system of detecting the intensity of light, which passed through the substrate, located under it. The substrate contains at least two zones, with a layer of oligonucleotides, non-specific to the nucleotide sequence under study, being immobilised on the surface of one of them, and a layer of nucleotides, specific to the nucleotide sequence under study, being immobilised on the surface of the other zone. The detection system contains at least two photosensitive independent sections, each of which is illuminated by the radiation, which passed through only one zone.EFFECT: device makes it possible to carry out the qualitative and quantitative analysis of the nucleotide sequences, increases the accuracy of the identification of the nucleotide sequences.3 cl, 5 ex

Sample plate // 2537234
FIELD: medicine.SUBSTANCE: group of inventions refers to medicine, namely to laboratory diagnostics. A sample plate comprises one or more wells having a base and one or more seats in the base and having a tapered hollow, as well as agent granules and microspheres inserted into the hollows. The agent granule or microsphere is substantially fixed inside the hollow by making a tight contact with the hollow taper or introducing therein along a friction fit, and forms a liquid-impermeable pack with the hollow taper. The group of inventions also refers to a method for using the plate for analysing the sample and to kits for analysing the sample, as well as to a method for making the above plate.EFFECT: group of inventions provides conducting various analyses in the single well, fixing the agent granules reliably in the required position, as well as enables reducing an amount of the liquid required for the analysis.11 cl, 18 dwg

Device and methods of detecting analytes in saliva // 2530718
FIELD: medicine.SUBSTANCE: group of inventions relates to a device and a method of detecting and/or quantitative determination of molecules in saliva and is intended for the determination of analytes in saliva. A microliquid device includes one or several areas of preliminarily processing, which include a surface, containing a molecule, capable of specific or non-specific binding with one or more of saliva components in a saliva sample, preventing the detection of one or more analytes, for specific or non-specific removal of a part of a fraction of the said saliva components in the saliva sample, preventing the detection of one or more analytes, with the molecule being hydroxyapatite or an antibody against mucin; and an area of detection, including a surface of a biosensor with the molecule, capable of specific binding of one or more analytes and providing the detection of one or more analytes, or one or more analytes and/or molecules, structurally relative to analyte and binding analyte-specific probes.EFFECT: application of the microliquid device provides the method of detecting the presence of one or more analytes.12 cl, 4 dwg, 4 ex

Analysis of troponin i with application of magnetic labels // 2530716
FIELD: medicine, pharmaceutics.SUBSTANCE: group of inventions relates to a method of measuring troponin I in a sample. For this purpose the sample is provided. After that, the sample is brought in contact with a monoclonal antibody to troponin I, bound with a magnetic label. Then, the sample is brought in contact with the polyclonal antibody or with two different antibodies to troponin I, bound with a sensor surface. The said antibodies are aimed at an amino acid sequence 30-110 of troponin I. After that, the detection of the magnetic label on the sensor surface is carried out. The group of inventions also relates to a device and a cartridge for measuring troponin I in the sample.EFFECT: inventions make it possible to carry out diagnostics of myocardium infarction within 5 minutes and with the application of the small-volume sample.14 cl, 2 dwg

ethod and device for quantification of target components with magnetic labels // 2530715
FIELD: measuring instrumentation.SUBSTANCE: group of inventions refers to quantification of a target component in a sample, where magnetic particles can have specific links with contact surface. Method of target component quantification in a sample with specific links of magnetic particles (2) to contact surface (4) of chamber (1) filled with the sample with kinetics depending on the amount of target component in the samples involves the following stages: a) at least two flushing stages when magnetic force affects magnetic particles (2) so that unlinked magnetic particles move away from contact surface (4); b) at least two measurement stages, each related to one of flushing stages, when total amount of magnetic particles (2) on contact surface (4) is measured during flushing stage a), while duration and/or number of further flushing stages are determined on the basis of measurement results from previous flushing and measurement stages; c) assessment stage when amount of the target component (2) in the sample is assessed by at least one measurement result from stage b). In addition, invention describes device of the same purpose, data carrier with PC software for implementation of this method, and application of this sensor device for molecular diagnostics and for biological or chemical sampling.EFFECT: improved reliability of quantification.14 cl, 9 dwg
ethod for preparing immunosorbent for diagnosing herpes simplex type i // 2528896
FIELD: medicine.SUBSTANCE: invention represents a method for preparing an immunosorbent for diagnosing herpes simplex type I virus involving preparing a polymer solid-phase carrier adsorbing a herpes type I virus antigen, aqueous 20% polyethylene glycol (PEG-20), adsorbing on the carrier in 0.2M carbonate-bicarbonate buffer at pH 9.6 of the lysate high-purity herpes type 1 virus antigen prepared of the herpes simplex type I virus strain - ATCC VR-539, washing, lyophilisation, incubation of the sorbed antigens with a blocking solution. Using the natural lysate high-purity herpes type I virus antigen ATCC-VR-539 enables providing considerably higher analysis sensitivity, tris-based blocking system ensures neutralising the undesired non-specified reactions. Besides, using the invention leads to better conditions of the agent activity on the surface of the solid-phase carrier, reducing the agent consumption.EFFECT: using polyethylene glycol in plate processing enables higher specificity and sensitivity of the kit.1 tbl, 1 ex

ethod for detecting analyte from particle solution and device for implementing it // 2528885
FIELD: medicine.SUBSTANCE: invention refers to biology and medicine, namely to qualitative and/or quantitative indication of analytes. A device for analyte collection from a solution by concentrating it on magnetic particles comprises a flow chamber consisting of upper and lower passages comprising electrodes for generating an electric field perpendicular to a fluid flow in a fluid passage from a semi-permeable dialysis membrane arranged between the upper and lower passages, a magnetic field concentrator and a magnet for generating a magnetic moment in the concentrator.EFFECT: ensured accelerated and higher detection sensitivity.3 cl, 1 dwg, 1 ex

Rotating magnetic field for improved detection in cluster analysis // 2528102
FIELD: medicine.SUBSTANCE: group of inventions refers to medicine, namely laboratory diagnostics and can be used for performing thecluster analysis. The analysis procedure involves the stages as follows: a) providing a suspension of superparamagnetic particles in a liquid to be analysed with the superparamagnetic particles coated with a biologically active agent; b) enabling the particles forming particle clusters with analytes found in the liquid; c) using a rotating magnetic field (B) with an angular frequency ensuring the magnetic field rotation only of the clusters having a size smaller or equal to a pre-set size, and d) detecting the rotating clusters. What is also presented is a device for performing the cluster analysis.EFFECT: group of inventions provides detecting the selectively activated clusters thereby increasing the analysis sensitivity.9 cl, 8 dwg
Immunological test for determining autoantibodies against testicular antigens // 2528060
FIELD: medicine, pharmaceutics.SUBSTANCE: present invention refers to an immunological test for the detection and specific determination of autoantibodies against testicular antigens which are associated with inflammatory genital disorders of male mammals in a biological sample of a male mammal, in particular the detection of testicular ER-60 autoantibodies and/or transferrin autoantibodies.EFFECT: presented immunological test enables detecting the presence of immunological and infection-related infertility effectively and simply in male mammals, particularly in humans.6 cl, 4 ex

Analysis device and method for performing biological analyses // 2527686
FIELD: medicine.SUBSTANCE: group of inventions refers to medicine, and can be used for multiplex analysis. An analysis device comprises a reaction space, two sets of individually coded microcarriers (2), and each microcarrier is functionalising, and each microcarrier of one of at least two sets has identical functionalisation, wherein the reaction space is a microchannel. The microcarriers (2) are shaped in relation to the microchannel (1) section which provides comprising two any microcarriers (2) arranged side by side out of contact with each other and with a perimeter of the microchannel (1) along the whole length of the microchannel (1). The device comprises the microcarrier (2) limit stop (4) longwise the microchannel (1), while fluids can still flow, and a code of the microcarriers is indicative of its functionalisation. The group of inventions also refers to a method for performing a multiplex analysis and the multiplex analysis microcircuit.EFFECT: group of inventions provides accelerated mass transfer, a decreased number of samples, simplifies preparing and performing the analysis and facilitates biological response readouts.17 cl, 18 dwg, 1 ex

Exciting magnetic spheres using feedback for ftir based biosensor // 2526198
FIELD: physics.SUBSTANCE: invention provides a method of controlling excitation of marker particles in a biosensor, particularly a biosensor employing frustrated total internal reflection (FTIR). When applying a predefined excitation force to the marker particles and determining the effect of the applied excitation force in space or on the binding surface of the sensor cassette of the biosensor device, control with excitation force feedback is used.EFFECT: providing a biosensor which is adapted to carry out the method according to the invention.9 cl, 2 ex, 3 dwg
agnetically controlled sorbent agent for bilirubin elimination from biological fluids // 2524620
FIELD: medicine.SUBSTANCE: invention refers to medicine and is applicable for treating endogenous intoxication caused by high plasma bilirubin concentrations accompanying various pathologies. Substance of a method: a surface of a magnetically controlled sorbent agent is coated with hydrophobic ligands either in the form of 3-mercaptopropionic acid methyl ester, or in the form of polymeric octadecylhydrosilicic acid, or in the form of polymeric hydrosilicic acid, containing covalently attached octadecyl ligands. Magnetite micro- and nanoparticles are presented by Fe3O4. The total bilirubin recovery rate in the sorbent agent is determined by a lyophilised bovine serum dry powder. The bilirubin content is determined by formula: AB(%)=100-(A460 nm test/A460 nm reference)×100, wherein AB(%) is a percentage of the absorbed bilirubin; A460 nm test is an optical density of the solution contacted the sorbent agent; A460 nm reference is an optical density of the solution before contact with the sorbent agent; 460 nm is a wave length whereat bilirubin has the maximum optical absorption.EFFECT: declared magnetically controlled sorbent agent possesses a developed specific surface, a reasonably good sorption capacity, as well as is characterised by a high degree of efficacy of bilirubin elimination from biological fluids, eg blood plasma (more than 30% lower bilirubin for one sorption cycle).7 cl, 3 tbl, 4 ex

Actuation with pulsed magnetic field for sensitive analysis // 2520607
FIELD: medicine.SUBSTANCE: group of inventions refers to medicine, namely to laboratory diagnostics, and may be used to control a magnetic or magnetisable object transfer in a biosensor cartridge. That is ensured by implementing the following stages of: (a) presenting the biosensor cartridge with a laterally extending sensor surface and at least a magnetic field generator to generate a magnetic field with the field gradient perpendicular to the sensor surface; (b) alternately actuating the magnetic field generator so that the generated magnetic field alternately direct the magnetic or magnetisable objects perpendicular to the sensor surface and thereto with pulse lengths of alternative actuation specified so that the above alternative actuation allows for no lateral motion of the magnetisable objects along the lateral direction of the sensor surface and (c) presenting a detection pulse with the magnetic field specified so that to allow for no transfer of the magnetic or magnetisable objects towards the gravity force. There are also presented biosensor systems.EFFECT: group of inventions provides more sensitive analysis ensured by the gravity force compensation.17 cl, 9 dwg
 
2550882.
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