Chemical analysis of biological material, e.g. blood, urine and testing involving biospecific ligand binding methods and immunological testing (G01N33/50)

G   Physics(393877)
G01   easuring; testing(233827)
G01N33/50                     Chemical analysis of biological material, e.g. blood, urine; testing involving biospecific ligand binding methods; immunological testing (measuring or testing processes other than immunological involving enzymes or micro-organisms, compositions or test papers thereforprocesses of forming such compositions, condition responsive control in microbiological or enzymological processes c12q)(1274)
Diagnostic technique for near-graft infection of joints // 2642659
FIELD: medicine.SUBSTANCE: invention relates to medicine, namely to traumatology and orthopedics and can be used to diagnose periprosthetic joint infection in endoprosthetics. Essence of method: conduct a microscopic examination of smear of aspirate, stained by Gram, and further measuring the concentration of D-lactate using the D-lactam test system, test sample is prepared by adding to the 25 mcl aspirate from the endoprosthetic joint 45 mcl of the enzyme mix and 80 mcl of the substrate mixta, turbidity control is obtained by adding 45 mcl of distilled water and 80 mcl of a substrate mix to 25 mcl of aspirate from the endoprosthetic joint; resulting mixtures are incubated in a thermostat at a temperature of 37 °C±1 °C for 30 minutes and measure the optical density of the mixtures on a plate reader using a 570–600 nm filter. Then optical density of each test sample is subtracted from difference in optical density of turbidity control and empty cell and for each test sample, the concentration is calculated from the calibration schedule and at a D-lactate value of 1.2 mmol/L and more diagnosed as periprosthetic joint infection.EFFECT: highly accurate method for the diagnosis of periprosthetic joint infection has been proposed, which allows fast, within one hour, to identify the presence of infection in the endoprosthetic joint, caused even by hard-to-cultivate microorganisms, which will allow to widely use the claimed method in the departments of traumatology, orthopedics and endoprosthetics.1 cl, 1 tbl, 1 ex
ethod for prediction of cryoglobulinemic vasculitis development in patients with chronic hepatitis c // 2642626
FIELD: biotechnology.SUBSTANCE: method for prediction of cryoglobulinemic vasculitis development in patients with chronic hepatitis C (CHC) is proposed. A sample of the patient's genomic DNA is obtained, polymorphisms are detected in the ITGB3 1565 T/C and PAI-I-675 5G/4G genes, followed by analysis of their combination. The risk of cryoglobulinemic vasculitis development is determined by the formula.EFFECT: invention allows to isolate a high-risk group for cryoglobulinemic vasculitis development among patients with CHC and to recommend intensive antiviral therapy for these patients.4 dwg, 7 tbl, 6 ex

ethod for chronic endometritis diagnostics at medium stage of secrection phase // 2642621
FIELD: medicine.SUBSTANCE: invention is intended for chronic endometritis (CE) diagnostics at the medium stage of the secretion phase. Pipelle biopsy of the endometrium using an atraumatic aspiration curette is performed to take a fragment of the uterine cavity mucous membrane at the medium stage of the secretion phase. By quantitative RT-PCR in real time, the mRNA level of the VEGFA gene is determined. At a level greater than 0.250, a conclusion is made about the presence of CE.EFFECT: effective diagnosis of chronic endometritis on the profile of genes mRNA expression in the endometrium during the implantation window in women with tubal peritoneal infertility.2 dwg, 3 ex

Sensor membrane with low temperature coefficient // 2641966
FIELD: chemistry.SUBSTANCE: glucose sensor is encapsulated containing the glucose oxidase enzyme contained within the crosslinked hydrophilic copolymer in contact with the surface of the electrode. The copolymer has the first units of the monomer of 2-hydroxyethyl methacrylate, the second monomer units of the structural formula (V), where Y is -O-; R2 is methyl; and z is an average value of 2 to 250; and the third units of the methacrylate derivatives containing hydrophilic cross connection of the formula (IIIa), where w is in the range of 0-10. A method for manufacturing a glucose sensor and a method for measuring the glucose level in an analyzed object are also disclosed.EFFECT: creating a sensor, in which cross-linked polymer has a permeability for glucose, not dependent on temperature, and gives the ability to generate analytical signals, which are beyond the control of temperature in the range of temperatures.23 cl, 9 dwg, 3 tbl, 4 ex

ethod for multi analyses of rare cells extracted or isolated from biological samples by filtering // 2641595
FIELD: medicine.SUBSTANCE: circulating cancer cells are isolated or extracted from a biological sample by vertical filtration through a filter with a pore size between 3 and 100 mcm that allows free circulating tumour rare cells to be retained, but does not prevent passage of smaller cells through the filter. The isolated cells are analyzed by cytomorphology, molecular analysis or DNA and/or RNA analysis. The results of determination are used to diagnose invasive cancer, to select targeted treatment and to monitor development of drug resistance.EFFECT: non-invasive biological sample test for early diagnosis of invasive cancer in patients with suspected malignant tumours.20 cl, 4 tbl, 3 dwg, 4 ex
ethod for determination of genetic predisposition to atherosclerosis, ischemic heart disease and myocardial infarction // 2641568
FIELD: medicine.SUBSTANCE: DNA is extracted from the patient's blood, mitochondrial DNA (mtDNA) is genotyped, the degree of mtDNA heteroplasmia is determined by the m.652delG mutation. With a value above 22.5%, high genetic risk is judged.EFFECT: increased effectiveness and accuracy of determination of predisposition to atherosclerosis, coronary artery disease and myocardial infarction.4 ex
ethod for prediction of risk of multiple organ failure syndrome development in patients after heart bypass // 2641033
FIELD: medicine.SUBSTANCE: method for prediction of the risk of multiple organ failure syndrome in patients after heart bypass surgery is proposed. Clinical and anamnestic indicators are analyzed and molecular genetic testing is performed with determination of polymorphisms of TLR6 and TREM-1 genes. A rating score is assigned to each prognostic criterion. The minimum risk is predicted with a score of 0 to 2.5. The average risk - with a score of 3.0 to 4.0 points. The high risk - 4.5 to 5.5 points.EFFECT: effective prediction of the risk of multiple organ failure syndrome after heart bypass by determining polymorphisms of candidate genes and calculation of the total risk by an assessment scale based on point equivalent.7 tbl, 3 ex

ethods and systems for gastrointestinal tract cancer risk assessment // 2640568
FIELD: medicine.SUBSTANCE: to assess the risk of gastrointestinal cancer, many classifiers are created in the database in accordance with the set of corresponding results of previous blood tests of many analyzed individuals. The assessmnet is carried out using the hardware risk processor for gastrointestinal cancer in the said target individual with the said combination of at least 10 different blood test characteristics using the said at least one classifier. Previous and current blood tests include the results of at least one of the following blood tests: RBC, hemoglobin (HGB), and hematocrit (HCT), and at least one of the following blood tests: analysis of hemoglobin mean content in an erythrocyte (MCH) and mean concentration of hemoglobin in an erythrocyte (MCHC). A quantitative measure of the gastrointestinal cancer risk is displayed on the client terminal.EFFECT: high accuracy of automated calculation of the quantitative risk factor for gastrointestinal cancer based on a set of blood test results.15 cl, 13 dwg
ethod for estimation of biomedical cellular products safety // 2640487
FIELD: medicine.SUBSTANCE: invention is intended to assess the safety of a biomedical cell product (BMCP). Control quantitative values are established that characterize the level of telomerase activity in cells with different tumourigenic potential. The levels of telomerase activity in the controls are compared. An evaluation of the safety of the test cells used for the production of BMCP or cells within the BMCP is conducted.EFFECT: increased accuracy due to the use of experimentally established control quantitative parameters - the level of telomerase activity in cells with different tumourigenic potential.2 cl, 3 ex
ethod for endogenous intoxication diagnostics in case of reperfusion syndrome after arterial circulation restoration // 2640187
FIELD: medicine.SUBSTANCE: while diagnosing endogenous intoxication in case of reperfusion syndrome before and after arterial circulation restoration, coefficient KACC/RN, reflecting the proportion of nitrogen-containing compounds (ACC) in residual nitrogen (RN) is calculated, according to the formula , where RN is residual nitrogen, U is urea, Cr is creatinine, UA is uric acid. If the specific gravity of nitrogen-containing compounds increases with respect to the residual nitrogen after arterial blood flow restoration in comparison with the parameters before restoration, development of endogenous intoxication is diagnosed.EFFECT: method allows to diagnose endogenous intoxication in case of reperfusion syndrome after arterial blood circulation restoration.1 tbl

Peptides suppressing respiratory viruse infections, their application and methods for obtaining // 2639559
FIELD: pharmacology.SUBSTANCE: inventions relate to a peptide synthesized chemically or genetically engineered, compositions comprising such a peptide, DNA coding a polypeptide, vector incorporating such a DNA, a host cell for expression of the peptide, a peptide screening Kit, capable of suppressing a respiratory virus infection and a method for screening of a peptide capable of suppressing a respiratory virus infection. The presented peptide contains 5 or more essential amino acids, 2 or more of these essential amino acids are located in the N-terminal or C-terminal region, and the N-terminal region contains a sequence of no more than 10 amino acids from the peptide N-terminal amino acid and the C-terminal region contains a sequence of no more than 10 amino acids from the peptide C-terminal amino acid, while the peptide consists of a sequence of amino acids, at least 90% identical to SEQ ID NO: 10.EFFECT: possibility of application of inventions to block infections of respiratory viruses such as influenza viruses or coronaviruses in the target cells for prevention and treatment of these infections.23 cl, 10 dwg, 3 tbl, 1 ex
ethod for diagnostics of severe liver fibrosis in patients with chronic hepatitis c with genotype 1 // 2639480
FIELD: medicine.SUBSTANCE: invention is a method for diagnostics of severe hepatic fibrosis in patients with chronic hepatitis C (HCV) of a natural course with genotype 1, characterized by activity of cytochemical enzymes lactate dehydrogenase (LDH), glucose-6-phosphate dehydrogenase (G-6-PDH) and nicotinamide adenine dinucleotide-diaphorase (NAD-diaphorase) determination in peripheral blood neutrophils and monocytes, and with a decrease in their activity in neutrophils and monocytes by more than three times compared to normal, severe liver fibrosis is diagnosed.EFFECT: method allows to detect liver fibrosis in chronic hepatitis C in patients who have not received treatment, with a possibility of timely initiation of antiviral therapy.6 tbl, 2 ex
ethod for caries prediction estimation by analysis of callekrein-4 gene polymorphism in mutation points g2664153a and g2142a in blood serum // 2639478
FIELD: medicine.SUBSTANCE: DNA is isolated from venous blood. To determine the point mutations of the KLK4 gene, the PCR method is used. In case of the presence of allele A in the mutation points G2664153A and G2142A of the KLK4 gene, caries development is predicted.EFFECT: allows to predict carious process in the absence of clinical signs of the disease.3 tbl, 2 ex
ethod for determination of direct causes of terminal outcome in case of ethanol poisoning at background of alcoholic liver disease // 2639435
FIELD: medicine.SUBSTANCE: to establish the direct causes of the terminal outcome for ethanol poisoning at the background of alcoholic liver disease (ALD) the complex of pathomorphological, forensic chemical and histochemical equivalents and their components is estimated in points. The points obtained are summarized, and for a total of 4-6 points, a brain type of dying is determined with the development of the cardiovascular and respiratory centres paralysis; for a total of 7-9 points, a hepatic type that causes acute liver failure, cerebral edema; for a total of 10-12 points a cardiac type in which the death is caused by the progression of cirrhotic cardiomyopathy (CCM) with an increase in heart failure of the left ventricular type; for a total of at least 13 points, a cardiac type based on alcoholic cardiomyopathy (ACMP), which leads to sudden cardiac death or acute circulatory insufficiency.EFFECT: method increases the accuracy of determination of the true cause of death and the reliability of information reflected in statistical documents.3 ex
ethod for differential diagnostics of hydrocephaly in newborns with extremely low body weight // 2639138
FIELD: medicine.SUBSTANCE: dead infants with extremely low body weight (ELBW) (from 500 up to 1000 g) are subject to immunohistochemical study of ventricular germinative zone, cytoarchitectonic field N6 projection, definition of the MMP-9 expression index (EI) in glioblasts, in addition, the reelin expression index in the Cajal-Retzius neurons is calculated. At zero values of both indices, hereditary hydrocephaly is diagnosed, at the MMP-9 EI equal to 0.25 conv. units or more, in combination with the reelin EI less than 2 conv. units - post-inflammatory hydrocephaly is diagnosed, at the MMP-9 EI equal to 0.17 conv. units or less, in combination with the reelin EI equal to 2 conv. units or more - posthemorrhagic hydrocephaly is diagnosed. The proposed method is highly-informative, implemented by the immunohistochemical method and allows to differentiate hereditary (associated with Sylvian aqueduct development abnormalities), post-inflammatory and posthemorrhagic hydrocephaly in dead infants with ELBW at the sub-cellular level. It is a morphological method for objective control of the clinical diagnosis quality, hydrocephaly treatment and the final clinical diagnosis.EFFECT: allows to optimize morphological hydrocephaly diagnosis depending on the etiological factor and considerably increases the quality of post-mortem diagnosis and epicurises.3 ex
ethod for estimation of professional risk to health related to arterial hypertension development for workers engaged in underground mining under conditions of labour with production noise at levels above permissible // 2639130
FIELD: medicine.SUBSTANCE: invention discloses a method for estimation of the professional risk to health related to arterial hypertension development for workers engaged in underground mining under conditions of labour with production noise at levels above permissible. The following laboratory indicators are determined in the blood of workers carrying out underground mining under the conditions of labour with productive noise above the permissible level: contents of high density lipoprotein, homocysteine, atherogenic index, malonic dialdehyde level, antioxidant activity level, vascular endothelium state through the degree of reduction of brachial artery diameter growth; in the case of confirmation for all laboratory parameters of the occupational conditioning, the probability pi1 of deviation of the vascular endothelium state and the probability pi2 of arterial hypertension development in workers with deviations from the normal state of the vascular endothelium are determined. The professional risk RiAG to workers health is calculated.EFFECT: increased method accuracy and reliability.2 tbl,1 ex
ethod for prediction of respiratory failure in patients with bronchial asthma // 2639122
FIELD: medicine.SUBSTANCE: genotyping of the polymorphic locus rs1837253 of the TSLP gene is carried out by the PCR method. When a CC genotype is identified, a risk of respiratory failure development in patients with bronchial asthma is predicted.EFFECT: obtaining of criteria for the prognosis of respiratory failure in patients with bronchial asthma.1 tbl, 2 ex
ethod for the determination of caffeine in biological material // 2638789
FIELD: biotechnology.SUBSTANCE: biological material is treated with acetone, the liquid extraction is separated by filtration, evaporated in an air stream at room temperature, the aqueous residue is diluted with water, saturated with ammonium sulfate, adjusted to pH 4.2-4.5, extracted once with a mixture of organic solvents of ethyl acetate and chloroform, taken in a volume ratio of 2:8, the organic extract is separated, chromatographed and physicochemically determined by calculating the quantitative content of the analyte, and characterised in that the treatment for a biological object, acetone is carried out repeatedly twice for 30 minutes, is extracted under conditions when the volume of the aqueous phase is equal to the volume of the mixture of organic solvents, before chromatography, the solvents are evaporated from the extract to obtain a dry residue, the residue is dissolved in a mixture of propanol-2-chloroform solvents taken in the ratio 5:5 by volume, chromatographed in a column of silica gel L 40/100 mcm using a mobile phase of propanol-2-chloroform in a ratio of 5:5 by volume, the eluate fractions containing the analyzed the substance is combined, the eluent is evaporated in an air current at a temperature of 18-22°C, then in a nitrogen stream until all the solvents are removed, the residue is dissolved in dichloromethane, as a physicochemical method, chromatography-mass spectrometry is used, the determination is carried out in a 25 m capillary column and an internal diameter of 0.2 mm with a fixed phase of 5% phenylmethyl polysiloxane using a helium carrier gas fed at a rate of 0.7 ml/min and a mass selective detector operating in the electron impact mode, the initial temperature of the column thermostat is 80°C, this temperature is maintained for 1 minute, then the temperature is programmed first from 80°C to 200°C at a speed of 40°C per minute, then from 200°C to 300°C at a speed of 12.5°C per minute, the temperature of the injector is 200°C, the interface temperature of the detector is 300°C, the intensity of the signal due to the charged particles formed during bombardment of the analyte that emerged from the capillary column and got to the ion source by an ionizing beam of electrons with an energy of 70 eV, the mass spectrum is recorded in full the equation of the calibration curve is calculated by the least-squares method using the method of least squares, which in this case has the form: S=5238700⋅C+43091, where S is the area of the chromatographic peak, and C is the concentration of the substance to be determined in the chromatographed sample, mcg, and the amount of caffeine is calculated by the area of the chromatographic peak.EFFECT: increased sensitivity of caffeine detection in biological material.4 tbl, 3 ex

ethod for artificial tooth bud production in vitro and obtained artificial tooth bud // 2638783
FIELD: medicine.SUBSTANCE: method for production of an artificial tooth bud in vitro involves: a) obtaining of selected mesenchymal cells of the dental pulp; b) dental pulp mesenchymal cells culturing in a monolayer on a surface for adhesive cells; and c) dental pulp mesenchymal cells culturing under non-adhesive conditions in culture vessels with culture surface with ultra-low attachment of cells to form a cellular unit, which is an artificial tooth bud. An artificial tooth bud obtained by the above method, a transplant containing such artificial tooth bud and a pharmaceutical composition for treatment of tooth fracture and/or damage or tooth loss, containing the above artificial tooth bud or transplant, and at least one pharmaceutically acceptable excipient is proposed as well. In addition, a method for in vitro screening of substances which modulate the dental tissue properties comprising the steps: obtaining of a sample of the above artificial tooth bud, or cellular unit, or tissue derived from it; sample division into pieces; incubation of at least one part with the substance subject to screening, and comparison of parameters measured for the treated part with another part, which is not incubated with the substance subject to screening.EFFECT: method allows to produce an artificial tooth bud from adult cells to eliminate the need for embryonic cells or tissues, and only one cell type is required, namely the selected tooth pulp mesenchymal cells, or two cell types, namely the selected tooth pulp mesenchymal cells and keratinocytes; without application of any artificial biological or nonbiological frame or media that do not come from cells used in the production of the specified artificial tooth bud.15 cl, 1 ex, 16 dwg

ethod for estimation of risk of cancer development in generations of population of radionucleous pollution regions // 2637623
FIELD: medicine.SUBSTANCE: peripheral blood of patients is taken, RNA is isolated, the samples of which are used to simultaneously quantify the expression level of the ST13, IER3, BRCA1, LRDD, MRAS genes of the p53 protein gene network, then the detected markers are compared for mothers and their offspring and in the presence of patients not exposed to the radiation factor, decrease or increase of the expression level of these genes in mothers with clinical manifestations of malignant neoplasms, and unidirectional changes of the level of these genes expression in their offspring, high risk for cancer is assessed for children.EFFECT: invention can be used for early diagnosis of the cancer risk in patients living in the radionucleous contamination region with one of the parents having a clinical picture of cancer.2 dwg, 2 ex

ethod for fetal hypoxia diagnostics in general prenatal hypoxic hypoxia model for rabbits // 2636891
FIELD: veterinary medicine.SUBSTANCE: general prenatal hypoxic hypoxia in pregnant Chinchilla rabbits is determined for a period of 27-28 days. To do this, they are placed in a hypoxic chamber containing 10±2% oxygen and 90%±2% nitrogen for 60 minutes. The animals are then slaughtered and hysterectomy is performed after 15 minutes, fetal sacs are opened, samples of amniotic fluid are collected. The concentration of lactate and urea is determined in these samples with the calculation of the lactate/urea ratio. At a lactate/urea ratio greater than 2.0-2.5 mmol/l, fetal hypoxia is diagnosed.EFFECT: increased accuracy of the fetal hypoxia diagnosis, which is a key task in solving the problem of creation and evaluation of the effectiveness of therapeutic strategies for fetal perinatal hypoxia.5 dwg
ethod for vaginal atrophy treatment in women in postmenopause considering vaginal epithelium and microbiocenosis state // 2636619
FIELD: medicine.SUBSTANCE: bio material is taken using a vaginal or urethral probe by scraping the vagina. The number of genomic equivalents of microorganism and their fraction in the total bacterial mass are determined using the "Femoflor-16" reagent kit by real-time PCR. If the proportion of Lactobacillus spp. is more than 80%, normocenosis, characterized by the dominance of normoflora is diagnosed. If the proportion of Lactobacillus spp. is less than 80%, dysbiosis is diagnosed. At vaginal atrophy against a background of normocoenosis hormonal therapy is appointed. At a vaginal atrophy against a background of dysbiosis hormonal therapy and lactic culture containing preparations are appointed.EFFECT: invention provides development of individual approaches to vaginal atrophy treatment in postmenopausal women, taking into account the state of the vaginal biocenosis.5 tbl, 3 ex
ethod and test system for determination of share of fetal dna in plasma of pregnant woman using high-productive sequencing methods // 2636618
FIELD: medicine.SUBSTANCE: invention is intended to determine the share of fetal DNA in the plasma of a pregnant woman using high-productive sequencing methods. 53 single nucleotide polymorphisms (SNP) located on 1-12 chromosomes with a heterozygote frequency of 40-50%, and specific primers to them are used. The share of fetal DNA is determined as a median of the fetal DNA share value for all informative SNPs.EFFECT: effective determination of the share of fetal DNA in the blood plasma of a pregnant woman, regardless of the sex of the fetus.4 tbl, 2 ex

ethods for personalized medical testing ex vivo for hematological neoplasms // 2636614
FIELD: medicine.SUBSTANCE: for ex vivo determination of patient's cells sensitivity to drugs, the whole sample (without preliminary purification and separation) of the hematologic tumour is divided into 35-100 aliquots to which drug compositions containing cytotoxic and non-cytotoxic compounds or combinations thereof are added. A cytometric platform is used to measure apoptosis or decrease in the number of malignant cells in the cell population. The manifestation of apoptosis of malignant cells is prescribed in the action of non-cytotoxic drugs.EFFECT: invention provides ex vivo testing for any number of possible combinations of drugs with subsequent polytherapeutic treatment.21 cl, 4 tbl, 33 dwg, 22 ex

Systems for individual dying // 2636531
FIELD: cosmetology.SUBSTANCE: method and system use one or more dispensers adapted to dispense the composition by individual order for the treatment of keratinous fibres, formulated, if desired, from tablets; an optical reader for obtaining sufficient characteristics of keratinous fibres, in order to make a realistic prediction of the treatment result; computing devices for predicting the treatment result, optionally, associated by means of an interface with the dispenser, and for selecting a custom treatment; as well as predicting the composition of tablets suitable for preparing a composition for treating keratinous fibres. Quickly-disintegrating tablets are proposed for use in the preparation of compositions for treating keratinous fibres.EFFECT: increased efficiency.19 cl, 47 dwg, 9 ex, 8 tbl

ethod of determining personal "implantation window" in women based on analysis of transcryption profile of genes // 2636527
FIELD: medicine.SUBSTANCE: method of determining personal "implantation window" in women involves measurement of expression levels of mRNA of functional human genes REIHER, DPP4, HLA-DOB, MSX1 in endometrial tissue samples obtained by pipelle biopsy in 7-8 day after the peak of luteinizing hormone. Further, the obtained values of mRNA expression levels are used to calculate the endometrial receptivity index. If the value of endometrial receptivity index is less than or equal to the threshold value, a conclusion is made about the incompatibility of endometrium of "implantation window" stage; if the value of endometrial receptivity index is greater than the threshold value, a conclusion is made about the correspondence of endometrium of "implantation window" stage, the threshold value is determined from the experimental data by ROC analysis.EFFECT: invention provides the cumulative assessment of violations of fertility and gives the ability to determine the personal implantation window through study only one biological sample, namely endometrial tissue, using only one research methods - real-time polymerase chain reaction.3 dwg, 1 ex
ethod for predicting risk of developing multiocular sclerosis in patients with subacute course of optic neuritis // 2636519
FIELD: medicine.SUBSTANCE: invention is designed to predict the development of multiocular sclerosis (MS) in patients with subacute course of optic neuritis (ON). In serum, the level of optical density for antibodies of IgM class to the antigen of the retina is determined. Magnitude of peak latency and amplitude of the R100 visual evoked potentials (VEP), amplitude of N95 pattern-electroretinogram (PERG), photopic negative response to the conicus ERG (FNR). When the level of optical density for IgM class antibodies to retina S-antigen is equal and more than 0.15, extending of peak latency of R100 VEP at 20% or more, reducing the amplitude of N95 ERG and/or amplitude of FNR to 20% or more the development of multiocular sclerosis is predicted.EFFECT: use of the invention makes it possible to provide specialized assistance in a timely manner in order to prevent the aggravation of the course of multiple sclerosis.3 ex

Compositions and methods for diagnosis and treatment of tumour // 2636461
FIELD: biotechnology.SUBSTANCE: variants of antibodies binding tumour-associated antigenic polypeptide TAT425 are proposed. Nucleic acids encoding the antibodies of the invention; a method for determining the presence of TAT425 protein in a sample and a method for diagnosing the presence of a tumour are also contemplated. The invention can find its further application in diagnosing and treating cancer.EFFECT: improved properties of the composition.26 cl, 10 dwg, 1 tbl, 16 ex
Oligonucleotide biochip for identification of genetic determinant of neisseria gonorrhoeae resistance to antimicrobial preparations, set of oligonucleotides used for immobilisation on biochip // 2636457
FIELD: biotechnology.SUBSTANCE: oligonucleotide biochip is proposed to identify the genetic determinants of N. gonorrhoeae resistance to antimicrobial drugs including fluoroquinolones, penicillin series antibiotics, cephalosporins, tetracyclines, macrolides, spectinomycin, and a set of oligonucleotides to be immobilised on a biochip. The biochip is a substrate with grouped elements containing 2 cells that do not contain probes to calculate the background signal, 3 cells with an immobilised marker for correct biochip positioning, as well as cells with immobilised oligonucleotides that determine the affinity of the analyzed DNA to N. Gonorrhoeae, and oligonucleotides providing identification of genetic determinants associated with pathogen resistance to antimicrobial agents, the oligonucleotides having the sequences of SEQ ID NO: 1-43.EFFECT: invention allows to accurately determine the genetic determinants of the compound resistance to antibacterial drugs in the clinical material in a short period of time.2 cl, 16 dwg, 2 tbl, 2 ex
ethod for detection of molecular-genetic markers associated with barrett esophagus progression // 2635964
FIELD: medicine.SUBSTANCE: methylation of the promoter regions of MGMT, CDH1, p16/CDKN2A, DAPK, RAR-β and RUNX3 genes in the esophagus mucosa samples obtained during biopsy in endoscopic study is determined by methyl sensitive PCR. If abnormal methylation of at least one of these genes is detected, a conclusion is made that the risk of oncological progression is increased by a factor of 10.EFFECT: increased accuracy in the diagnosis of the risk of malignant epithelium of the esophagus.1 dwg, 1 tbl, 1 ex
ethod for prediction of atopic dermatitis development in infants // 2635768
FIELD: medicine.SUBSTANCE: according to the invention, the intensity of histamine production is determined in children at risk for the occurrence of allergopathology by strains isolated from the intestine using a qualitative method, and when a histidine decarboxylating strain with a high intensity of symptoms (3 and 4 points), or more than two strains with insignificant intensity (1 and 2 points) are detected, development of atopic dermatitis in a child is predicted.EFFECT: timely detection of the risk of atopic dermatitis in infants.2 tbl
ethod for prediction of relapse development in patients with oral mucosa cancer // 2635535
FIELD: medicine.SUBSTANCE: in the tumor tissue taken during surgery, the level of the transcription factor kappa-B - NF-κB rb5 is determined and at its value of 16.0 CU/mg of protein per well and more, a high risk of relapse in a period of 6 to 24 months is predicted.EFFECT: prediction of the risk of disease recurrence, objective control of the malignant process development, the assumption of relapses development before their clinical manifestation and inclusion of patients into risk groups for intensive surveillance, timely measures for adjuvant treatment appointment.2 ex
ethod for predictiction of early recurrence of superficial bladder cancer // 2635534
FIELD: medicine.SUBSTANCE: method including an enzyme immunoassay and immunohistochemical examination of the tumour tissue is described, where the level of matrix metalloproteinase-2 is determined in patients with superficial bladder cancer in the morning urine collected prior to the operation, the ratio of the enzyme to the level of excreted creatinine, as well as in the tumour tissue removed during the operation is calculated, the level of matrix metalloproteinase-2 expression is determined. When the metalloproteinase-2 content is 7.3 pg/mcmol of creatinine and more in urine, and is 52% and more positive cells in the tumour tissue, a high risk of recurrence in a period of 3 to 12 months is predicted.EFFECT: prediction of the risk of disease recurrence, objective monitoring of malignant process development, a possibility of patients inclusion into risk groups for intensive monitoring, timely measures for appointment of adjuvant treatment.2 ex
Biomarkers for prediction of sensitivity to antitumor treatment // 2635193
FIELD: pharmacology.SUBSTANCE: invention is intended for treatment of cancer in a patient having AKT1 mutation at position L52 or D323. The presence of L52 or D323 mutation in the patient's AKT1 gene is determined. A patient with such mutation receives (S)-2-(4-chlorophenyl)-1-(4-((5R,7R)-7-hydroxy-5-methyl-6,7-dihydro-5H-cyclopenta[d]pyrimidine-4-yl)piperazin-1-yl)-3-(isopropylamino)propan-1-one or a pharmaceutically acceptable salt thereof.EFFECT: invention provides effective cancer treatment.17 cl, 18 dwg, 3 tbl, 4 ex
ethod for determination of liver disease form in case of severe alcoholic poisoning // 2634640
FIELD: medicine.SUBSTANCE: following signs are distinguished: alcohol concentration in the venous blood of the femoral, portal and inferior vena cava, portal vein diameter, the number of liver vessels with a diameter of 0.3-0.5 cm per unit area of 25 cm2, the thickness of the muscular wall of the left ventricle. The activity of alcohol-oxidizing enzyme systems is also determined: alcohol dehydrogenase in the hepatocytes of the beams region and in the hepatocytes of the central vein region; microsomal ethanol-oxidizing enzyme system: in periportal hepatocytes, in hepatocytes of the beams region, in hepatocytes of the central vein region, catalase-peroxidase system in the hepatocytes of the central vein of the liver acinus and the corresponding coefficients of classification functions. Further, 5 linear classification functions are used to determine the liver disease form f(1-5), using the mathematical expression. The obtained values of the classification functions are compared among themselves and the larger value of f in the series f(1-5) is used to predict the form of liver disease and the cause of death - general toxic or toxic effect of ethanol associated with heart or liver damage.EFFECT: method allows to identify the form of alcoholic liver damage and clarify the immediate causes of death in severe ethanol poisoning during forensic examination of corpses due to the definition of biochemical indicators.1 tbl, 2 ex
ethod for determination and prediction of woman's health in perimenopause and postmenopause // 2633926
FIELD: medicine.SUBSTANCE: severity of hot flashes symptoms and severity of sweating symptoms is determined on a 10-point visual-analogue scale. Deficiency of melatonin secretion is detected using a questionnaire test for melatonin deficiency. The metabolite of 6-sulfatoxymelatonin is determined in two portions of urine. Serum is analyzed and the follicle-stimulating hormone, luteinizing hormone, estradiol, prolactin, total testosterone, thyroid-stimulating hormone, insulin, glucose of blood are determined. The degree of climacteric syndrome severity is assessed. The period of woman's life is determined. The expected life expectancy is determined. The biological age is determined. The quality of life is assessed using the SF-36 questionnaire in conjunction with WHQ. In addition, progesterone is determined for women in the period of perimenopause. For women in the postmenopausal period, genitourinary menopausal syndrome and late metabolic disturbances are revealed.EFFECT: method allows to reliably determine the state of a woman's health in the climacteric period due to the evaluation of the complex of the most significant indicators.8 cl, 3 ex
ethod for hepatitis b virus dna identification in biological material with low viral load based on two-stage pcr // 2633755
FIELD: medicine.SUBSTANCE: method for hepatitis B virus identification at a low viral load is proposed by virus DNA determination in a bioassay using the PCR method with further fragments sequencing. Detection is carried out in plasma and/or serum, blood clots, oropharyngeal washings, sperm, liver tissues. Identification is carried out in two stages. At the first stage, asymmetric PCR with extended primers is used (direct primer: 5'-ctgcgcaccagcaccatgcaactttttcacctctgc-3', reverse primer: 5'-cagaccaatttatgcctacccccca-3'). The second stage uses primers to one of the four regions of the HBV genome.EFFECT: increased specificity and sensitivity of hepatitis B virus diagnosis.4 ex, 2 dwg, 2 tbl

ethod for molecular diagnostics of mitochondrial pathologies based on pyrosequencing technology // 2633752
FIELD: medicine.SUBSTANCE: pyrosequencing reaction is conducted with a system consisting of a forward primer, a reverse primer labeled with biotin, and a sequencing primer. A total of four primer systems were developed for four types of mtDNA mutations m.11778G> A, m.8993T> G, m.3243A> G, m.8344A> G, associated with hereditary mitochondrial diseases, presented as lyophilized substances.EFFECT: invention provides molecular genetic detection of pathogenic mutations of mitochondrial DNA associated with hereditary mitochondrial pathologies, and determination of heteroplasmia level from these mutations.2 cl, 2 dwg, 1 tbl

ethod of determining content of free gibberellic acid in vegetative plant organs by method of capillary electrophoresis // 2633486
FIELD: chemistry.SUBSTANCE: invention can be used to determine the content of free gibberellic acid (GA3) in vegetative plant organs of an apple, grapes, winter wheat. For this, samples of biological material are extracted with ethyl acetate, and then the extract is filtered and evaporated at 40°C. The dry residue is then dissolved in 60% aqueous acetone. Quantitative determination of free gibberellic acid content is carried out by capillary electrophoresis in a quartz capillary with effective length of 0.5 m and an internal diameter of 75 mcm with positive voltage polarity. A solution containing 0.33 wt % of boric acid, 0.05 wt % of sodium tetraborate and 0.5 vol. % of isopropanol, pH = 9.3 is used as an electrolyte. Detection is carried out at a wavelength of 254 nm.EFFECT: quantitative determination of gibberellic acid in plant raw materials.1 tbl, 3 dwg, 3 ex
ethod for determining physical efficiency of students // 2633346
FIELD: medicine.SUBSTANCE: subject is tested on a bicycle ergometer. After carrying out the bicycle ergometry, a bioimpedance study of the composition of the subject's body, loading tests in accordance with the standard of the GTO (Ready for Labour and Defense), a spectrophotometric urine analysis are carried out. The obtained results are assigned with native points. After that, the number of final points is calculated by the mathematical formula. The obtained result provides a basis to draw a conclusion about the satisfactoriness degree of physical efficiency.EFFECT: method allows to increase the objectivity of determining the student's physical efficiency by determining the degree of muscle mass development, analyzing the performance of certain muscle groups and the body's functional reserves which are spent to perform physical activity.7 tbl, 1 ex

ethod for processing tumour cells // 2632429
FIELD: biotechnology.SUBSTANCE: method for processing tumour cells with a composition containing sodium dichloroacetate (DCA) and caffeine sodium benzoate is proposed. The measurement of antitumour activity is based on the distribution of cells by DNA content, which confirms the apoptosis of tumour cells. The effectiveness of the combined effect is assessed by the level of cells with less DNA than the diploid set (sub-G1 population) that result from the fragmentation of the nuclei of apoptotic cells.EFFECT: invention provides high antitumour activity and selectivity of sodium dichloroacetate while reducing toxic effects in non-tumourous cells.8 dwg, 1 ex

ethod for determination of predictors of sinoatrial node preserved function in patients with long-term persistent atrial fibrillation // 2631601
FIELD: medicine.SUBSTANCE: essence of the method: for patients with long-term persistent atrial fibrillation, prior to operation while probing the heart cavities or intraoperatively after connecting the cardiopulmonary bypass, but prior to the main stage of the operation, without introduction of sympathomimetic drugs, blood samples are taken from the ascending aorta and coronary sinus with determination of the norepinephrine, metanephrine and normetanephrine level. When the threshold values for norepinephrine in the coronary sinus exceed 819.9 pg/ml, metanephrine in the ascending aorta exceeds 18.83 pg/ml, normetanephrine in the ascending aorta exceeds 15.27 pg/ml, as predictors of the preserved function of the sinoatrial node, such patient is subject to surgical treatment of long-term persistent atrial fibrillation with a high degree of efficacy. Use of the invention provides an increase in the effectiveness of surgical treatment of long-term persistent atrial fibrillation.EFFECT: predictability of the effectiveness of surgical treatment of atrial fibrillation and the release of patients from the need for conducting pacing and antiarrhythmic therapy in the postoperative period.1 ex
ethod for early diagnostics of reproductive capability of meat cows // 2630986
FIELD: veterinary medicine.SUBSTANCE: reproductive qualities of meat cattle cows in terms of productivity are determined according to the elemental composition of wool. Concentration of chemical elements of copper, iodine, selenium and zinc is determined, and at copper concentration of 5.51-7.57 mcg/g, iodine concentration of 0.332-0.575 mcg/g, selenium concentration of 0.496-0.797 mcg/g, zinc concentration of 116.0-146.0 mcg/g, reproductive qualities of cows are estimated as high, the animals are good at bulling and inseminated, at copper concentration of 3.54-5.50 mcg/g, iodine concentration of 0.111-0.331 mcg/g, selenium concentration of 0.348-0.495 mcg/g, zinc concentration of 90.0-115.0 mcg/g, reproductive qualities are assessed as low, animals are not good at bulling, not inseminated, require rations correction in terms of these elements.EFFECT: method allows to simplify evaluation of the reproductive ability of cows.2 tbl
ethod for diagnostics of congenital nemaline myopathy // 2630982
FIELD: medicine.SUBSTANCE: invention is a method for nemaline myopathy diagnostics in which a sample of a biopsy specimen of skeletal striated muscle tissue is taken from a patient with clinical picture of congenital myopathy, immunohistochemical detection of a factor induced by 1-alpha hypoxia (HIF-1α) is performed, for which histological specimens are cut, a fluorescent signal amplifier is applied, then a blocker protein is applied to the sections to reduce background non-specific staining, then the slices are incubated with the rabbit polyclonal antibodies to HIF-1α. After washing, the respective secondary fluorescent antibodies are applied to the cuts, coated with a photoconductive reagent, and luminescent microscopy is performed, and nemaline myopathy is diagnosed under the muscle fiber envelope when round and/or oval red-coloured loci 3-5 mcm in diameter are detected.EFFECT: increased reliability of nemaline myopathy diagnosis.2 dwg, 2 ex
ethod for early diagnostics of cervical intraepithelial neoplasias (cin) based on metabolic markers study // 2629632
FIELD: medicine.SUBSTANCE: invention is intended for early diagnostics of cervical intraepithelial neoplasias (CIN). Cervix pathology is identified. The level of predictor markers expression is determined by means of a Western blot analysis with chemiluminescence detection and real-time PCR. At expression levels of HIF1alpha 0.029, GLUT1 782.9±156.5, IGFBP-3 0.038±0.0076, HK2 36.4, VDAC1 70, CIN1 (LSIL) is diagnosed. At expression levels of HIF1alpha 0.03, GLUT1 1154.2±88.7, IGFBP-3 0.02±0.005, HK2 44.9, VDAC1 87, CIN2-3 (HSIL) is diagnosed.EFFECT: effective diagnosis of cervical intraepithelial neoplasias at earlier stages.5 dwg, 1 tbl, 3 ex
African swine fever prevention method // 2629399
FIELD: veterinary medicine.SUBSTANCE: proposed African swine fever prevention method, including the identification of animals with the infectious disease at the initial stage of development, the slaughter of the patients and further examination of the other animals. The rest of the animals in the African swine fever virulent disease site are examined during the day by the polymerase chain reaction with fluorescence detection in the real time. The animals virus carriers and healthy animals are moved to the separate premises and the premises sanitation with ozone-air mixture is provided in their presence: in a room with animals virus carriers - during one month not less than 3-4 times once a week with ozone concentration 4-6 g/m3 during 20-30 minutes, in the room with healthy animals - during not more than two weeks 2-3 times per week with ozone concentration of 3-4 g/m3 during 15-20 minutes.EFFECT: expansion of African swine fever prevention method.2 tbl, 1 ex
ethod for forecasting neurologic disorders in newborns with delay of internal development of fruit // 2629395
FIELD: medicine.SUBSTANCE: invention can be used to predict neurological disorders in neonates with intrauterine growth retardation (IGR). The essence of the method: the glutamic acid content and the insulin-like growth factor-1 are determined in the umbilical cord. With a glutamic acid value of less than 17.08 pg/ml and an insulin-like growth factor-1 below 30.2 pg/ml, neurological development in a neonate is predicted.EFFECT: increasing the accuracy and specificity of predicting neurological disorders in newborns with a delay in fetal development of the fetus and in a timely manner to carry out pathogenetic therapy.2 ex
ethod of rationale of biomarkers produced due to negative effects from influence of harmful production factors on workers of industrial manufactures // 2629351
FIELD: medicine.SUBSTANCE: quantitative and qualitative characteristics of the HPF affecting workers is determined. A list of likely workers' NE exposed to the installed HPF is established. An observation group (OG), which workplaces are influenced by the HPF is formed. A comparison group (CG), the workplaces of which are not influenced by the HPF is formed. Sampling of blood of the workers of the OG and CG are performed. The priority HPF is detached and a list of likely NE from its impact is established. The degree of cause and effect relationship of the development of the established NE from the impact of the priority HPF on the calculation of the relative risk index and in terms of production etiological fraction (EF) of the contribution of factors of working conditions in the development of NE. Special NE types is detached for which EF is more than 51.0% and among them the leading NE is detached based on the largest EF. Based on the established leading NE, a list of traditional laboratory indicators (LI) and additional LI is formed for it. Traditional and additional LI of the OG and CG workers is determined. Some of the indicated LI is detached, the level of which significantly differs from the level of the CG indicators, and they establish a specified NE. The functional indicators (FI) of this specified NE of the OG and CG workers is determined. The most informative FI in the OG are detached, the level of which significantly differs from the level of such FI in the CG. A quantitative assessment of the degree of cause-effect relationship of the FI of the specified NE with the identified priority HPF is performed. The more specified FI are detached, for which EF is more than 51.0%. The dependencies are modeled in the system of "exposure of the priority HPF-LI of the specified NE" through the determination of the calculated probability P'of the deviation LI of the specified NE from the norm. The modeling of the dependencies between the LI of the specified NE is carried out, for which a reliable dependence "exposure level of the priority HPF-LI of the specified NE" is established, performed separately from the OG and CG workers. Marker LI are detached. Separately for each marker LI, a modeling dependencies by the method of the non-linear regression analysis "marker LI - specified FI" is performed using the determining the calculated probability P" taking into account the duration of exposure of the investigated priority HPF. That marker LI, for which a reliable probability of deviation from the standard of the specified FI is established, is taken as a justified BM of the productive justified NE at the impact of the HPF on the workers.EFFECT: high accuracy.3 cl, 8 tbl

ethod for noninvasive prenatal diagnostics of fetal aneuploidy // 2627673
FIELD: medicine.SUBSTANCE: method for non-invasive prenatal diagnosis of fetal aneuploidy is proposed, which includes extraction of extracellular DNA (ecDNA) from a blood sample of a pregnant woman, selection of genome regions for amplification, preparation of genomic libraries, mapping of the resulting sequences to a reference genome or part of the human genome with coordinates determination, determination of the coverage value for each genome region, characterized by the openness of chromatin between the placenta and mother's blood cells, differing not less than 20%, and obtaining genome regions with the indicated openness of chromatin, after which a conclusion is made about the presence of fetal aneuploidy. A method is proposed to obtain genome regions for carrying out the above noninvasive prenatal diagnosis of fetal aneuploidy.EFFECT: simple and economical way of prenatal diagnosis of fetal aneuploidy in the early stages of pregnancy.7 cl, 3 dwg, 3 tbl, 5 ex

ethod for step-by-step diagnostics of type 1 hereditary tyrosinemia in children // 2627647
FIELD: medicine.SUBSTANCE: for children of the first 3 months of life who have a combination of a symptom complex consisting of fever of unknown origin, edema, jaundice and dyspeptic syndrome, and children aged 4 months and older - hepato- or hepatosplenomegaly and clinical manifestations of acute rickets, a blood test is performed to assess the hemoglobin level and the number of erythrocytes, the number of platelets, the level of ALT, ACT, bilirubin and its fractions, the level of alkaline phosphatase, calcium, phosphorus, AFP, coagulogram. In case of detected anemia, thrombocytopenia, elevated ALT, ACT, bilirubin, AFP and laboratory signs of acute rickets, a study is made of the level of tyrosine in blood by tandem mass spectrometry and a study of succinyl acetone in blood and urine. In case of an increased tyrosine level, as well as an increased succinylacetone level in the blood (above 2 mmol/l) and more than 2 mmol/l of creatinine in the urine, a genetic test is carried out in terms of FAH gene mutations.EFFECT: use of the method allows to diagnose HT1 at the early stages with high accuracy.7 dwg, ex 1
 
2550974.
Up!