Preparation of polysaccharides not provided for in groups and c08b0001000000-c08b0035000000 and derivatives thereof (C08B37)

ethod for extracting pectin substances from sugar beet production wastes // 2628435
FIELD: food industry.SUBSTANCE: method for extracting pectin substances includes washing the raw materials with water, grinding, sonication, hydrolysis and extraction, precipitation of pectin substances and their purification from the pectin-containing extract with ethyl alcohol. The sonication is carried out at the fixed frequency of 350 Hz, with the subsequent hydrolysis and extraction at the temperature of 55°C with the 1% solution of ammonium oxalate for 45-50 minutes. The obtained pectin-containing extract is purified and pectin substances are precipitated with ethyl alcohol with the addition of sodium chloride salt in the amount not exceeding 10 g per litre of the alcohol. Wherein the sonication is carried out for 20 minutes at the temperature of 40°C.EFFECT: invention allows to increase the yield of pectin and does not require the use of strong mineral acids as an extractant.3 ex
ethod for producing low-molecular chitosan and chitosan oligomers // 2627870
FIELD: chemistry.SUBSTANCE: method for producing low-molecular chitosan and chitosan oligomers by a chemical depolymerization method, including hydrolysis of chitosan in the presence of an acid, followed by filtration, fractionation, purification and drying of products, characterized in the fact that hydrolysis of chitosan is carried out with 2.5-12.5% dilute nitric acid at a temperature of 70°C, followed by separation of the hydrolyzate into two fractions - a precipitate of low-molecular chitosan and a mother liquor, then chitosan oligomers are precipitated from the mother liquor during addition of isopropyl alcohol and cooling. Then, both products are washed with isopropyl alcohol and air-dried, finally, the products of chitosan depolymerization are redissolved in water and dried lyophilically.EFFECT: method is proposed for producing a product of a narrow fractional composition which is highly soluble in water.5 ex
ethod for producing nanocrystallites of low-molecular chytosan // 2627540
FIELD: chemistry.SUBSTANCE: method involves dispersing in water of high molecular weight chitosan with a molecular weight of 190-250 kDa with vigorous stirring, subsequent treatment in a 9.00-17.00% aqueous solution of sulfuric acid at 100-120°C for 20-55 minutes for homogeneous hydrolysis. The hydrolysis product is then precipitated from the sulfuric acid solution by introducing a 10% sodium hydroxide solution into the hot solution at a volume ratio of sodium hydroxide to sulfuric acid of 1:0.667. The product obtained is washed and dried at room temperature.EFFECT: invention makes it possible to obtain low molecular weight chitosan with a molecular weight of 5-20 kDa with a high degree of crystallinity, thermal stability, antimicrobial activity and biological compatibility.1 tbl, 3 ex

High-effective method for bacterial polysaccharide production, preparation of conjugate based thereon and immunogenic composition // 2627156
FIELD: biotechnology.SUBSTANCE: method for production of serogroup X Neisseria meningitidis capsular polysaccharide, its conjugate with a carrier protein and an immunogenic composition based on the conjugate for preparation of a vaccine against meningitis. The method is implemented using a fermentation medium comprising casaminoacid, an optimal strategy of nutrient solution addition and an improved process for polysaccharide isolation and purification, exclusive of any chromatographic methods. The polysaccharide and protein ratio in the conjugate is in the range of 0.2 to 0.6. Inventions allow to obtain purified serogroup X Neisseria meningitidis polysaccharide in a yield of 300 to 550 mg/l, average molecular weight of 400 to 550 kDa, yield of 60% to 65% at the purification stage and containing less than 0.5% of protein, less than 0.5% of nucleic acids and less than 5 EU/mg of endotoxins. X polysaccharide is adjusted to a size of 150 to 200 kDa and conjugated to a carrier protein.EFFECT: increased efficiency.9 cl, 4 dwg, 11 tbl
ethod for obtaining flax polysaccharides // 2625583
FIELD: food industry.SUBSTANCE: aqueous extract of polysaccharides is obtained by extraction under the influence of mechanical oscillations with the frequency of 10-20 Hz and the amplitude of 3-6 mm within 14-16 minutes. The extract is then cleaned and dried.EFFECT: invention allows to intensify the process of flax polysaccharides extraction, to improve the product quality, to reduce energy costs, to shorten the process duration, to improve the working conditions in comparison with the known production technology.1 cl

ethod of processing inulin-containing raw material with obtaining food inulin powder and method of obtaining ultra-pure inulin // 2623739
FIELD: food industry.SUBSTANCE: method comprises soaking and washing the raw material, cutting, drying, and grinding. The cut raw material is dried by blotting the surface moisture and subjected to infrared drying to the humidity of 10-12%, with the subsequent holding for 4-6 hours. The dried raw material is ground to the particle size of less than 100 mcm, and the resulting powder is re-dried to the humidity of 5-10%. Then it is divided into fractions according to the particle size: less than 15 microns - the first one, 15-25 microns - the second, and 25-100 microns - the third. The second fraction is dried to the humidity of 5-6% and subjected to re-grinding, followed by selecting the particles of less than 15 microns that are combined with the first fraction and used as food inulin. The residues of the second fraction are combined with the third fraction and used for producing ultra-pure inulin by preparing the aqueous suspension, holding it for at least 1 hour, and centrifuging. From inulin separated from membranes, suspension is prepared. The resulting suspension is cleaned from proteins with solutions of sodium chloride and sodium hydroxide, with the subsequent centrifugation, filtration and depigmentation of the obtained extract on anionite. Fructans are then concentrated and precipitated from the pre-heated concentrate with a solvent, preferably ethyl alcohol, taken in a volume equal to 1.5-2.5 of the concentrate volume, with the subsequent filtration, washing with ethanol and vacuum drying of the finished product.EFFECT: invention allows to obtain inulin by the dry method and to improve the efficiency of complex processing of inulin-containing raw materials.6 cl, 3 dwg
ethod of sulfate of xilane wood of birch with sulfamic acid // 2620597
FIELD: chemistry.SUBSTANCE: in the method of the invention, xylan sulfate is obtained by sulfating xylan with sulfamic acid in N,N-dimethylformamide. In N,N-dimethylformamide, with stirring, sulfamic acid, urea, xylan are added and kept with stirring at a temperature of 100°C for 0.5-6.0 h. The resulting reaction mass is neutralised with a 3% aqueous solution of sodium hydroxide and concentrated in vacuo until the solvent is completely removed. Then, the dry residue is dissolved in distilled water and filtered. The obtained filtrate is purified by dialysis. The aqueous solution of the sodium salt of xylan sulfate is concentrated in vacuo until the water is completely removed.EFFECT: increased method efficiency.7 ex, 1 tbl
ethod of sulfate of birch wood xilane // 2620595
FIELD: chemistry.SUBSTANCE: method for sulfating xylan birch wood with chlorosulfonic acid in an organic solvent is characterized in that the sulfation is carried out in 1,4-dioxane at a temperature of 20-40°C for 3-6 hours, and xylan sulfate is isolated after neutralisation of the reaction mass with a 3% aqueous solution of sodium hydroxide, followed by purification by dialysis.EFFECT: effective way of sulphating xylan.1 tbl, 9 ex

Arabinogalactan polysaccharide production method // 2620013
FIELD: food industry.SUBSTANCE: invention refers to food and medical industry. The proposed method implies that arabinogalactan is extracted during 30-40 minutes in an ultrasonic device with frequency 27-42 kHz at a temperature of 20-30°C with water duty of 1.3-7 to the dry weight of the raw material, then the extract is filtered, dispersed by ultrasonic spraying and concentrated in a receving tank up to dry matter concentration within 20-40%. The concentrate is precipitated by the addition of organic water-mixed solvent at the ratio 1:4. The precipitated arabinogalactan is washed in the same solvent, dried and ground. It is preferable to extract arabinogalactan without pre-extraction of dihydroquercetin. The arabinogalactan extract is condensed in serially connected vessels to obtain arabinogalactan with specified molecular weight. After arabinogalactan precipitation and washing, the extraction agent is filtered, distilled and reintroduced to the arabinogalactan manufacturing process.EFFECT: invention allows for the production of arabinogalactan of high purity with specified molecular weight and additional presence of dihydroquercetin, reduction of power inputs and of extraction process duration.4 cl, 1 dwg, 2 tbl, 3 ex
ethod for obtaining inulin from wild plants mixture // 2619758
FIELD: food industry.SUBSTANCE: method of obtaining inulin from chicory and elecampane is proposed, including preparing the inulin-containing raw material, its mechanical cleaning, washing the roots, rhizomes, stems, their grinding and mixing. The mixed and shredded pieces of raw material are extracted twice with hot water at the temperature of 75°C for 2-3 days at constant stirring. The resulting inulin extract in mass is treated with 96% ethanol at a ratio of 1:1 by volume, with the subsequent deposition of inulin at the temperature of minus 16°C. The resulting inulin is intended for use as a food additive in manufacturing functional meat, flour and dairy products.EFFECT: quality improvement.1 ex
ethod for producing pectin-containing product from flax grain // 2619385
FIELD: food industry.SUBSTANCE: method for producing a pectin-containing product from flax grain includes moistening flax grain, its softening and treatment by IR-rays. Moistening the flax grain to the humidity of 16-18% is carried out at the temperature of 18-20°C and softening durability of 6-8 hours. The treatment with IR rays is carried out at the wavelength of 0.9-1.1 mcm and the radiant flux density of 20-22 kW/m2 within 70-90 seconds before the grain reaches the temperature of 150-160°C and the final humidity of 7-8%.EFFECT: enhanced quality and high biological value.5 ex
ethod for oxidized dextran obtaining // 2618341
FIELD: pharmacology.SUBSTANCE: method involves hydrogen peroxide addition to dextran aqueous solution to a final concentration of 0.25 to 5.0% in the solution, depending on the desired depth of dextran oxidation. The mixture is stirred, poured into a container with a flat inert surface and distributed in a thin layer. Simultaneous oxidation of dextran and drying of the desired product is performed at 90-95°C for 1-2 hours. Preferably, a 10% aqueous dextran solution with a molecular weight of 40 kDa or 70 kDa is used. Pallets made of glass, aluminium alloys, stainless steel, with ceramic or Teflon coating are used as an inert surface. Preferably, mixture layer thickness before oxidation and desired product drying is 1-3 mm.EFFECT: improved method.4 cl, 2 tbl, 3 ex

Non-anticoagulant glycosaminoglycans with repeating disaccharide units, and their medical application // 2617764
FIELD: pharmacy.SUBSTANCE: invention refers to chemically modified glycosaminoglycan, which is heparin or heparan sulfate with anti-factor IIa activity less than 10 IU/mg, anti-factor Xa activity less than 10 IU/mg and average molecular weight from 4.6 to 6.9 kDa.EFFECT: invention also refers to a method for production of modified glycosaminoglycan and its medical application.45 cl, 3 dwg, 4 tbl
Chitosan production method // 2615636
FIELD: agriculture.SUBSTANCE: chitosan production method is proposed, comprising grinding of insect puparia, alkaline treatment of chitin-containing raw materials with constant strirring at elevated temperature and further washing of the remainder with distilled water. Musca domestica puparia are used. Alkaline treatment is carried out at a temperature of 80°C with 1-1.5% alkali. Two-stage treatment is additionally performed with 3.5-5% hydrochloric acid and 4.5-5% sodium hydroxide while stirring for 1.5-2 hours at each stage, followed by filtration and washing of dry extract obtained after each processing stage with distilled water. Puparia are pre-washed with water from foreign substances before grinding.EFFECT: increased finished product output.2 tbl, 3 ex

Alkylated cyclodextrin compositions and processes for preparing and using the same related applications // 2615385
FIELD: pharmaceutics.SUBSTANCE: group of inventions relates to pharmaceutics and provides a process for producing sulfoalkyl ethers of cyclodextrin ensuring a low content of the components, degrading the drug in particular the chlorides due the stage of processing by activated carbon with a conductivity of 10 µs or less, as well as to the product, obtained using said process and composition for delivery of active agents sensitive to the content of chlorides.EFFECT: group of inventions allows to reproducibly obtain sulfoalkyl esters of cyclodextrin with reduced chloride content and provides improved stability of the composition with active agents that are sensitive to chloride content.33 cl, 10 dwg, 13 tbl, 21 ex
ethod of producing heparin // 2612813
FIELD: chemistry.SUBSTANCE: method of producing heparin involves thawing and homogenisation of raw material, filling with 0.6M sodium chloride solution, adding alcalase to a final concentration of 0.1-0.5%. Pigs' frozen mucous membrane of the small intestine (mucosa) with a shelf life of no more than 6 months at -20°C is used as raw material. Enzymatic hydrolysis is being performed at 65°C and pH 8.5-8.6 for 6 hours, then the enzyme is being inactivated at 90°C for 15 minutes. The produced hydrolyzate is cooled to temperature of 15-20°C and centrifuged. Concentrated hydrochloric acid is added to supernatant fluid to pH 3.0, heated to 70°C and being held for 30 minutes for additional hydrolyzate decontamination of protein origin ballast substances. The resulting residue is removed by way of centrifugation. Thereafter, end product sorption is performed in dynamic mode from the supernatant fluid on anion exchanger YMC-BioPro Q75. Anion exchanger is pre-equilibrated by 0.6M sodium chloride solution. After the sorption is complteted the anion exchanger is washed with 0.6M sodium chloride solution, ballast substances are removed from the anion exchanger with 0.9 M sodium chloride solution, heparin is desorbed from the anion exchanger with1.4 M sodium chloride solution.EFFECT: invention increases output of heparin, its specific activity, reduces the technology process.1 cl, 5 ex
ethod to produce pectin from plant raw materials // 2610312
FIELD: food industry.SUBSTANCE: invention relates to food, pharmaceutical and biochemical industries. Method of pectin production includes extraction of pectin-containing pomaces by solution of food or mineral acid with pH of 0.8-3.2 and hydraulic module of 1:(15-45) for 1-6 hours at temperature of 70-95°C, filtration of produced extract, its treatment. Then extract is concentrated on a cascade of ion-exchange and adsorption columns, ultrafiltration and diafiltration plants with polymer membranes with cut off at 20-100 kDa. Produced pectin solution is dried at temperature of 45-95°C to produce pectin in drying belt vacuum deviced with subsequent grinding of pectin or devices of spray type. Besides, removal of ballast substances in ion exchange and adsorption processes is carried out on cation exchange resins AMBERLITE® FPC23 H, anion exchange resins AMBERLITE® FPA90 Cl and adsorption resins AMBERLITE® FPX66.EFFECT: invention makes it possible to increase yield of pectin, extent of purity and to produce pectin with specified molecular mass.7 cl
Agent for binding fats obtained from biomass, formed during brewing // 2608233
FIELD: food industry.SUBSTANCE: invention relates to polysaccharides rich composition containing beta-glucan, chitin and chitosan, extracted from Saccharomyces cerevisiae cell wall from biomass, which is brewing process by-product. A method for preparing the composition involves the following stages: reactor preparation with NaOH solution with concentration from 0.25 to 3 M while stirring and temperature from 50 to 95 °C, addition biomass produced during brewing to said solution, maintenance of said conditions for at least 1 hour. Then, said solution is cooled down to room temperature, solution neutralization by at least single adding of acidic solution or water until pH 7. At that, in case of more than single adding stage of solid product separation from said solution is carried out between additions. Then solid product transfer to at least single washing with water is carried out and separation of obtained solid product, solid product drying to constant mass and fine grinding. Obtained composition can be prepared in form of edible, pharmaceutical or veterinary product.EFFECT: invention enables to obtain composition, which is capable of selective fats binding, and therefore it can be used for preventing and/or treating diseases, such as overweight, obesity, hypercholesterolemia, hypertriglyceridemia, hypertension and cardiovascular disorders.13 cl, 5 dwg, 18 tbl, 9 ex

ethod of producing water-soluble polysaccharides of common burdock roots // 2604934
FIELD: chemistry.SUBSTANCE: method comprises exhaustive extraction of starting material with water, separation of plant material, deposition of water-soluble polysaccharides, washing and drying. Starting material used is common burdock roots ground to linear size of 0.2-0.5 mm . Extraction is three-fold with hot purified water in ratio of raw material and extractant of 1:30 when processing in ultrasonic bath with frequency 35 kHz for 30 minutes at a temperature of 80 °C. Plant material is separated by filtration, and water-soluble polysaccharides are deposited three-fold amount of 95 % ethanol while stirring, cooling in freezing chamber at temperature -18 °C for 1 hour. Precipitate is filtered through a dry ash-free paper filter under vacuum at residual pressure of 0.4-0.8 atm. Filter cake is washed successively with a solution of 95 % ethyl alcohol in purified water (3:1) with ratio of raw material and solution of 1:15 and mixture of ethyl acetate and 95% ethyl alcohol (1:1) at a ratio of raw material and solution of 1:10. Filter with precipitate is then dried at a temperature of 100-105 °C to constant weight.EFFECT: invention reduces duration of extraction and increases output of water-soluble polysaccharides.1 cl, 3 dwg, 2 ex
ethod for producing agar // 2603912
FIELD: food industry.SUBSTANCE: invention relates to food industry. Disclosed is a method for producing agar, including its extraction from algae by ionic liquid, represented by the deep eutectic with melting point which is lower than 100 °C. At the end of the extraction process, the produced solution is being separated into algae residue and the agar solution in the deep eutectic, and alcohol is being added in the latter. Then, the hot solution is being filtered and separated into agar and alcohol-containing solution of the deep eutectic, from which the latter is regenerated and reused. Alga Ahnfeltia is used as an alga, preliminary cleaned from impurities, by electrohydraulic treatment in dilute alkali solution, then, inner steam destruction of algae thallomes is being performed, then, treated algae is being extracted by the deep eutectic. Alcohol, deep eutectic and water are being regenerated and reused, besides, the agar, produced by filtering, is being dehydrated.EFFECT: invention ensures producing high-purity agar, and the process duration is considerably reduced.9 cl
ethod of producing water-soluble oligosaccharides // 2600133
FIELD: food industry.SUBSTANCE: invention relates to food industry. Method of producing water-soluble oligosaccharides from hardwood shavings involves extraction of oligosaccharides at temperature of 170 °C, separation by filtration from shavings and centrifugation. Extraction of vegetable raw materials is carried out with treated hot water for 120 minutes, with subsequent separation on a chromatograph, to produce end product. Extraction is performed from birch or aspen shavings, with ratio of water to shavings of 1:30, method includes performing repeated extraction at ratio of water to shavings of 1:10, and separation, to output of end product, is carried out using gel-penetrating chromatograph.EFFECT: method increases output of water-soluble oligosaccharides.1 cl

ethods and systems for production of sugars from carbohydrate-rich substrates // 2600126
FIELD: chemistry.SUBSTANCE: method comprises providing carbohydrate-rich substrate and aqueous solution with hydrolysis catalyst. Evacuation pressure is maintained above substrate for introduction of hydrolysis catalyst into carbohydrate-rich substrate to produce reaction mixture. Said reaction mixture is exposed to high pressure of gas phase at step of hydrolysis reaction, reaction mixture is held to produce monomers and/or polymers of sugars in open space inside fibres of substrate. Reaction mixture is exposed to pressure of gas phase, which is lower than pressure of hydrolysis reaction, for removing said monomers and/or polymers of sugars from said open space inside fibres of substrate into said aqueous solution and held. Steps of hydrolysis and extraction are repeated in at least two cycles of changing pressure to extract maximum amount of monomers and/or polymers of sugars from open space inside fibres of substrate. Monomers and/or polymers of sugars are then separated from aqueous solution. Method does not include treatment with water vapour or breakdown with ammonia of fibres of carbohydrate-rich substrate.EFFECT: invention simplifies method of extracting sugars from carbohydrate-rich substrates.25 cl, 1 dwg, 5 tbl, 7 ex

ethod for obtaining chitosan // 2600005
FIELD: food industry.SUBSTANCE: present invention can be used for producing a natural chitosan biopolymer from waste generated by industrial processing of shellfish. Shell-containing raw material of shellfish is preliminarily mixed with water in the ratio of 1:15 and milled with simultaneous deproteinization by electrohydraulic shocks created by ultra-long discharges. Demineralisation of the precipitate is performed with a hydrochloric acid solution with the volume ratio of 2-4 % at the water duty of 1:10 and the temperature of 20-25 °C and continuous mixing for two hours. Precipitate is separated from the supernatant, washed to pH 7.0, then the obtained chitin is soaked in the sodium hydroxide solution with the weight ratio of 35-45 % for 1-2 hours at 95-98 °C.EFFECT: invention ensures a shorter process of chitosan production.1 cl, 1 dwg, 1 tbl, 2 ex

ethod of producing coordination compounds of oligogalacturonic acid with biogenic metals (ii) as delivery systems of biogenic metals (ii) and systems for removing heavy metals (ii) // 2599494
FIELD: medicine.SUBSTANCE: invention relates to pharmacology, and describes a method of producing coordination compounds of oligogalacturonic acid with biogenic metals (II). Method involves production of pectin-containing preparation of oligogalacturonic acid with polymerisation degree equal to no less than 10, subjected to reaction with metal salt (II): copper, cobalt, iron, zinc, manganese, magnesium in aqueous medium at neutral pH, exposing reaction mixture at room temperature, neutralisation of formed acid, water flushing, centrifugation and drying precipitate. According to invention to 1 l of 5.0·10-3 M aqueous solution of purified oligogalacturonic acid while stirring is added first 2 M aqueous solution of ammonia hydrate to pH 8, then 1 l of 4.5·10-2 M aqueous solution of metal acetate (II); formed gel-like precipitate is treated with 96 % ethanol at volume ratio of 1:2, held for 2 hours.EFFECT: invention ensures delivery of biogenic metals (II), as well as removal of heavy metal cations (II) from body.1 cl, 8 tbl, 4 dwg, 6 ex

Complex hyaluronic acid esters, their production and use thereof in dermatology // 2598552
FIELD: chemistry.SUBSTANCE: complex esters of hyaluronic acid and hydroxycinnamic acid derivatives are proposed. With that, hydroxycinnamic acid derivatives are selected from ferulic acid and caffeic acid. Method of producing esters of hyaluronic acid involves dissolving sodium salt of hyaluronic acid in formamide. With that, the said salt is introduced into the reaction in the presence of an equivalent amount of tertiary hydroxycinnamic acid derivatives with base pre-activated carbonyldiimidazole at the room temperature. Obtained viscous mass is diluted with water solution of NaCl, the reaction product is extracted by deposition of acetone followed by purification of methanol, filtration and drying in vacuum.EFFECT: invention allows obtaining esters of hyaluronic acid and hydroxycinnamic acid derivatives used for skin protection as the compounds that reinforce elasticity with moisturizing, softening action.15 cl, 6 dwg, 1 tbl, 9 ex

ethod for producing sialic acid-containing sugar chain // 2597975
FIELD: biotechnology.SUBSTANCE: group of inventions relates to biotechnology. Disclosed are methods of producing sialylated sugar chain. Method comprises reacting a sugar chain with CMP-sialic acid in presence of ST6Gal-I and phosphatase temperature from 20 °C to 37 °C for 8-48 hours to produce triantennary or tetraantennary N-linked sialylated sugar chain, containing sialic acid on each of its non-reducing ends. In another version, before said step, method involves reaction of sugar chain with UDP sugar in presence of glycosyltransferase. In third version, method involves reaction of agalacto-biantennary complex sugar chain with UDP-GlcNAc in presence of MGAT4 and MGAT5. Obtained product reacts with UDP-Gal in presence of β4GalT1, then with CMP-sialic acid in presence of ST6Gal-I and phosphatase.EFFECT: invention enables to obtain triantennary or tetraantennaryα2,6-sialylated on each of their non-reducing ends sugar chains with high output in single-reactor synthesis.15 cl, 9 dwg

ethod of functionalising surfaces for detecting analytes // 2597768
FIELD: chemistry; instrumentation.SUBSTANCE: group of inventions relates to diagnostics, specifically to a device for detecting analytes, including plastic substrate, partially or completely directly coated with binding polymers, fixed on substrate noncovalently, wherein said binding polymers contain polysaccharide frame equipped with: aromatic groups of form -X-CONH-Z, groups of carboxylic acid of form -X-COOH and reactive groups F, having form -X-CONH-Z′, where X denotes a straight or branched, substituted or unsubstituted alkyl chain containing from 1 to 6 carbon atoms, Z denotes an aryl function, Z′ denotes a group which is capable of binding with other molecule, as well as methods for production of said device, and also to binding polymer and method of its production.EFFECT: group of inventions enables to obtain a device for detecting different analytes.20 cl, 13 dwg, 34 ex

Implanted matrix material for regenerative medicine and method of its production (versions) // 2597085
FIELD: medicine.SUBSTANCE: invention relates to medicine, particularly to a new matrix material for tissue bioengineering and regenerative medicine and methods of its production. Matrix material is designed based on vegetal polysaccharide - pectin with etherification degree of not more than 50% and proteins of intracellular matrix-collagens I and IV types, which contains said components in the following concentrations: 0.5-2.0 wt%, 0.1-1.5 wt% and 0.01-0.5 wt%, respectively. Method of production of the implanted matrix material for regenerative medicine in the form of hydrogel involves mixing at a temperature of 5 °C max of calcium acetate solution of type I collagen to final concentration of 0.1-1.5 wt% with type IV collagen solution to the final concentration 0.01-0.1 wt%, then the produced mixture is mixed with previously prepared gel formation initiator, including calcium chloride solution in the concentration, providing a gel forming process, non-toxic buffer system in amount sufficient for neutralisation of the mixture, and sodium chloride solution up to physiological concentration; then the pectin solution is introduced with degree of esterification of 50% max to the final concentration 0.5-2.0 wt% and the gel is formed by temperature rise up to physiological. Invention also relates to a method of producing implanted matrix material for regenerative medicine in the form of hydrogel, which involves mixing at a temperature of 5 °C max of calcium acetate solution of type I collagen to final concentration of 0.1-1.5 wt% with IV type collagen solution to the final concentration 0.01-0.1 wt%, then the produced mixture is neutralised, stabilised by the non-toxic buffer system and the sodium chloride is added up to physiological concentration then the pectin solution with etherification degree of not more than 50 % to the final concentration 0.5-2.0 wt% is added, to obtain a fluid composition, from which the gel is formed.EFFECT: invention enables to obtain a biocompatible implantable materials with a low rate of biodegradation and ability to stimulate regenerative process, in particular, recovery of nerve conductors after a brain injury.10 cl, 8 dwg

Adducts of chitosan dodecahydro-closo-dodecaborate with nitrates or perchlorates of magnesium or aluminium and method for production thereof // 2596741
FIELD: chemistry.SUBSTANCE: invention relates to chemistry of compounds of chitosan dodecahydro-closo-dodecaborate B12H122−−anion, chitosan, magnesium and aluminium salts, specifically to adducts of chitosan dodecahydro-closo-dodecaborate with nitrates or perchlorates of magnesium or aluminium and a method for production thereof. Synthesised novel products may be used as energy-intensive additives of various compositions, for example pyrotechnical substances or primers. Adducts of composition (C6O4H9NH3)2B12H12×nMgA2 or (C6O4H9NH3)2B12H12×nAlA3, where 0<n<1, A−NO3− or ClO4−.EFFECT: wherein adducts have optimum properties, where n is equal to 0,18 ÷0,25.2 cl, 3 dwg, 4 ex

Glucan containing compositions // 2596496
FIELD: medicine.SUBSTANCE: group of inventions refers to medicine and pharmaceutics, namely, to immune stimulating composition in the form of a gel having the melting point of gel transition to sol above 37 °C and containing from 0.1 to 6 % of soluble beta-glucan, which is obtained from yeast and includes the main chain of β-(1,3)-bound glucose residues and side chains containing two or more β-(1,3)-bound glucose residues, herewith the side chains are connected to the main chain with β-(1,6)-link, and in fact does not contain repeating β-(1,6)-bound glucose residues; and a gelling agent, which represents carboxymethylcellulose or gellan gum, as well as to a method for its production and use to facilitate wound or ulcer healing, to treat oral mucositis, to treat cancer and to ensure skin cells proliferation in vitro.EFFECT: group of inventions provides a synergetic effect between glucan and the gelling agent.28 cl, 5 ex, 5 tbl, 9 dwg

ethod of producing low-molecular water-soluble chitin in electron-beam plasma // 2595162
FIELD: technological processes; chemistry.SUBSTANCE: invention relates to method of producing low-molecular water-soluble chitin. Method provides for treatment of high-molecular chitin powder with particle size of 10-100 mcm and viscosity-average weight of 1,000 kDa. Treatment is carried out in electronic beam plasma-chemical reactor with installed inside the working chamber reactor electromagnetic system in low-temperature strongly nonequilibrium electron-beam oxygen plasma, or steam, or nitrogen for 1-60 minutes at pressure of plasma forming medium 5-10 Torr, accelerating voltage 30 kV, current electron beam 1.5-2.5 mA, temperature in reaction volume of 50-90 °C.EFFECT: invention allows to produce sterile low-molecular water-soluble chitin without formation of toxic side compounds emissions and drains, polluting environment.1 cl, 1 dwg, 12 ex

ethod of producing pectin // 2593479
FIELD: food industry. SUBSTANCE: invention relates to food industry. Pectin production method envisages washing before hydrolysis-extraction of milled pectin-containing raw material with drinking water for 0.5-1.5 hour with simultaneous mixing at 10-30 rpm and t = 40-60 °C, separation of the obtained mixture into solid and liquid phases, mixing the solid phase with purified water in the ratio of 1:10-1:40. Herewith the drinking water is preliminarily purified on a two-stage reverse osmosis plant. Mixture obtained after washing is heated up to 50-80°C with simultaneous stirring and circulation, hydrolysis-extraction is performed by cavitation treatment in a rotary-oscillatory apparatus with further separation of pulp into solid and liquid phases in a vacuum drum filter. Obtained liquid phase is centrifuged with the relative centrifugal force of FR = 2,000-5,000 g followed by microfiltration at P = 0.1-0.3 MPa and V = 0.06-0.16 l/sec, ultrafiltration at P = 0.2-0.6 MPa and V = 0.15-0.30 l/sec, diafiltration at P = 0.2-0.6 MPa and V = 0.1-0.27 l/sec till pectin substances concentration reaches 1-2 %. Herewith ultrafiltration and diafiltration are carried out on tangential filters with ceramic membranes. Then it is thickened in a vacuum-evaporator till dry substances content reaches 3-8 % and spray-dried at the air temperature of 150-200 °C. EFFECT: output of the pectin is 70-84 % of theoretically possible one. 1 cl, 4 tbl, 3 ex

Inclusion complex of disulfiram and cyclodextrin // 2592625
FIELD: pharmaceutics. SUBSTANCE: invention refers to pharmaceutical industry and deals with inclusion complex of disulfiram (DSF) and cyclodextrin (CD), selected from a group consisting of unsubstituted cyclodextrin (uCD) or substituted cyclodextrin, such as methylcyclodextrin (MCD), dimethylcyclodextrin (DMCD), trimethylcyclodextrin (TMCD) and hydroxypropylcyclodextrin (HPCD) produced by the method, in which CD, taken in a solid or dissolved in water or alcohol form, when stirred at a temperature from 20 to 70 °C is combined with DSF in the form of a solid substance, fusion or alcohol solution, where alcohol is selected from methanol, ethanol, n-propanol or iso-propanol, where molar ratio of CD and DSF ranges from 2.01:1 to 2.40:1. Obtained mixture is cooled to 0-25 °C, subjected to sterilising filtration and lyophilized, or solvent is evaporated. EFFECT: reception of water-soluble inclusion complex of disulfiram with cyclodextrin, which can be extracted with high degree of purity. 1 cl, 5 dwg, 2 tbl, 8 ex

ethod of dextranal producing // 2592618
FIELD: technological processes; chemistry. SUBSTANCE: invention relates to a method of dextranal producing. Method envisages modification of dextran with molecular weight 20-75 kDa in the form of 5-25 % aqueous solution by mechano-activation processing initial dextran in apparatus with energy of activation of 16-85 kJ/mol. Preferably dextranal is extracted from its aqueous solution by ethanol and dried. EFFECT: invention enables to obtain easily soluble derivative of dextran for pharmaceutical, cosmetic and food industries, eliminating harmful impurities and simultaneously eliminating loss of product. 1 cl, 1 tbl, 3 ex
ethod of oxidised dextran producing // 2592617
FIELD: technological processes. SUBSTANCE: invention relates to method of oxidised dextran producing. Method involves oxidation of aqueous solution of dextran with molecular weight of 20-75 kDa with potassium permanganate solution in medium of acetic acid, settling and filtering solution from impurities, separation of oxidised dextran with ethanol and drying. At that, initial dextran in form of 20-25 % acetic acid aqueous solution in concentration of 32-34 % in amount of 0.005-0.1 % of initial volume of dextran solution, potassium permanganate solution in concentration of 2 % in amount of 20-25 % of weight of dry dextran in solution, and ethanol is in concentration of 95-96 % with ethanol to liquid ratio of (1.4-1.6):1. Preferably dextran oxidation is carried out at temperature of 60-70 °C, filtering of oxidised dextran solution from impurities is carried out on membrane micro filter, and drying of oxidised dextran is carried out in vacuum oven at temperature of 60-85 °C. EFFECT: invention enables to obtain oxidized dextran with higher output due to creation of conditions for maximum possible release of all fractions of oxidised dextran, not only medium and high-molecular but and low-molecular ones, eliminating loss of oxidised dextran and simultaneously increase organoleptic properties of oxidised dextran and its solubility in water. 4 cl, 3 ex
ethod of producing granules of cross-linked chitosan // 2590982
FIELD: chemistry.SUBSTANCE: invention relates to polymer materials and specifically to a method of producing granules of cross-linked chitosan, which involves cross-linking chitosan with glutaric aldehyde using hydrochloric acid solution containing glutaric aldehyde, with molar ratio of chitosan:hydrochloric acid:glutaric aldehyde, equal to 1:(0.5-1.0):(0.1-1.0), and then extrusion moulding gel in form of threads, which are mechanically cut into granules and dried at temperature 40-70 °C for 1-2 hours.EFFECT: invention provides decrease in method of amounts of acid, glutaric aldehyde and precipitation agents, enables to obtain chitosan granules of controlled size.1 cl, 6 ex

ethod of producing cross-linked gel // 2590558
FIELD: cosmetology.SUBSTANCE: invention relates to method of producing cross-linked gel polysaccharide and use thereof to long period of filling volume of skin defects. In method of obtaining aqueous gel containing polysaccharide in crosswise-cross linked form together with bifunctional or multifunctional epoxy cross-linking agent, at temperature below 35 °C. Obtained mixture is held at temperature lower than 35 °C during at least one hour. Cross-linking reaction of prepared mixture is stimulated. Then, if necessary restored formed cross-linked gel.EFFECT: invention ensures production of gels with improved viscoelastic properties.14 cl, 3 dwg, 2 tbl, 3 ex

Compositions and methods of aloe polysaccharides // 2587784
FIELD: pharmaceutics.SUBSTANCE: invention relates to pharmaceutical industry, specifically to fine powders of aloe and use thereof. Method of preparing fine-grained powder of aloe extract, which includes weighing freeze-dried aloe powder, where freeze-dried aloe powder has at least 25 % polysaccharides aloe; dissolution of freeze-dried aloe powder in deionised water to prepare a solution; addition of organic solvent in solution to obtain a first mixture; settling of first mixture to produce precipitate; extraction of part of supernatant fluid from first mixture and addition of organic solvent in amount exceeding volume of supernatant solution to obtain a second mixture; treatment of second mixture in a centrifuge; tracking appearance of sediment in second mixture; addition of organic solvent in first mixture, if there is formation of any sediment in second mixture; pumping supernatant fluid of first mixture, where pumping occurs only in case there is no any sediment in second mixture; filtration sediment from first mixture; extraction of powder aloe extract; placing aloe extract powder in flask for freeze-drying in freezing unit; freeze-drying frozen aloe extract powder in freeze-drying unit; and grinding freeze-dried aloe extract powder in the device for grinding to fine-grained texture, organic solvent is selected from a group of methanol, ethanol, isopropyl alcohol, propanol, under certain conditions. Use of fine-grained powder extract of aloe vera in manufacture of a medicament with immunomodulating and anti-cancer properties. Injectable composition having immunomodulating and anti-cancer properties, containing an extract of aloe vera and one or more pharmaceutical preservatives. Use of injection composition for preparing a drug for treating one or more cancer diseases, selected from a group comprising leukaemia, lymphoma, prostate cancer, breast cancer and colon cancer. Use of the injection composition for preparing a drug for immunomodulation or immune stimulation in a human with disturbed immune system or immune disease.EFFECT: aloe extract powder described above is characterised by content of polysaccharides with high molecular weight, possesses pronounced immunomodulatory and anticancer properties.37 cl, 7 dwg, 9 tbl

Hyaluronic acid derivatives capable of forming hydrogels, synthesis method thereof, hydrogels based on said derivatives, method for production and use thereof // 2586931
FIELD: chemistry.SUBSTANCE: invention relates to new derivative of hyaluronic acid of general formula (I), method for production thereof, to hydrogel based on it, to method of producing hydrogel and to use of hydrogel for producing preparations for cosmetic products, medicine or regenerative medicine. At that, Ar is phenyl and R1 is ethylene or Ar is indole and R1 is ethylene or Ar is indole and R1 represents carboxyethylene. R2 represents alkyl with 3-7 carbon atoms, and n is ranges from 1 to 7,500.EFFECT: invention enables to obtain material, sufficiently strong and elastic, not exhibiting significant changes of biological and physical properties and forming strong hydrogel.12 cl, 1 dwg, 1 tbl, 18 ex

ethod of producing chitosan-based films using ion cross-linking reagents // 2586697
FIELD: biotechnology.SUBSTANCE: invention relates to processing polymers and biomedicine, in particular to development of chitosan-based insoluble but swellable materials with low toxicity and controlled release of drug compounds. For making films chitosan is dissolved in acetic acid, then 1.0-5.0 % solution of chitosan is added with ion cross-linking agent potassium pyrophosphate and/or sodium tripolyphosphate. Ionic cross-linking reagents with concentration of 1.0-2.5% are added to chitosan solution at the stage of producing molding solution at room temperature or with concentration of 3.0-5.0 % at temperature 60 °C and molar ratio of ionic compound-amines of chitosan equal to 0.029-0.121 mol/mol. Film can also contain myramistin in amount of 0.5 % of the weight of chitosan.EFFECT: this method allows to produce insoluble in water, but highly swellable chitosan films, ensuring prolonged therapeutic effect when added with biologically active compounds.1 cl, 2 dwg, 9 ex
ethod for preparing composition based on modified sodium hyaluronate, composition based on modified sodium hyaluronate and use thereof // 2582702
FIELD: chemistry; medicine.SUBSTANCE: invention relates to a method for preparing a composition based on modified sodium hyaluronate and use thereof in various fields of medicine, veterinary medicine and cosmetology. Method of preparing a composition based on modified copolymer of sodium hyaluronate and heparin comprises forming transverse covalent bonds between hydroxyl groups residues of D-glucuronic acid and D-N-acetylglucosamine included into composition of sodium hyaluronate, and residues of α-D-glucosamine and uronic acid related to heparin, by introducing active cross-linking bifunctional agent - (polyethylene glycol) diglycidyl ether (PEGDE) in an alkaline medium. After chemical copolymerisation, reaction with cross-linking agent is stopped. Obtained reaction mixture is neutralised to pH 7.0, then subjected to tangential filtration using a membrane with cut-off threshold 0.22 mcm, concentrated and stirred until complete homogenisation. Starting material used is animal-derived sodium hyaluronate of pharmacopoeial purity, subjected to tangential ultrafiltration to remove low-molecular fragments capable of triggering inflammatory response.EFFECT: biocompatibility, biodegradability and non-immunogenicity of modified compositions based on sodium hyaluronate allows use thereof in various fields of medicine and veterinary medicine, including surgery and arthroplasty, orthopedics, traumatology, ophthalmology, dermatology, aesthetic medicine and cosmetology.1 cl, 4 dwg, 10 ex
ethod of producing pectin and cellulose from beet pulp // 2580884
FIELD: chemistry.SUBSTANCE: method for production of pectin and cellulose from beet pulp involves hydrolysis of milled beet pulp in the presence of hydrochloric acid while heating, squeezing the solid phase, multistage solid phase extraction, precipitation of pectin from liquid phases, followed by purification, dehydration and drying of pectin and cellulose. From raw beet pulp the pectin substances are extracted at the first stage by the first acid extraction with 0.3% solution of hydrochloric acid at pH~1.6-1.8 for 1 h at 90°C using hydromodulus 1:200. Second stage involves the second acid extraction with 1.2% solution of hydrochloric acid at 90°C using hydromodulus 1:200 during 4 hours. Obtained washed coarse cellulose of beet pulp after the second acid extraction is subjected to single-step alkaline extraction with 0.5% solution of sodium hydroxide at 90°C during 1 h using hydromodulus 1:100; for further treatment of cellulose from residues of pectin substances, hemicellulose, residual lignin and lipids.EFFECT: invention enables to obtain cellulose and pectin in series from fresh beet pulp subject to complete processing.1 cl
ethod of octa-galact-uronide production // 2576535
FIELD: chemistry.SUBSTANCE: method of producing octa-galact-uronide includes acid hydrolysis of pectin with subsequent separation of the liquid phase from insoluble residue of pectin and separating octa-galact-uronide. Pectin is used as low-esterified pectin with an esterification degree not exceeding 30 %. Acid hydrolysis is carried out in 0.1-2.0 mol/l solution of mineral acid at temperature 70-100 °C till concentration of octa-galact-uronide in liquid phase is not less than 0.25 mg/ml. To extract octa-galact-uronide from liquid phase, it is neutralised up to pH 4.0 minimum. Then calcium salt is added to the concentration of 0.06-0.12 mol/l. Then the sediment of octa-galact-uronide is separated from the liquid phase and dried.EFFECT: invention enables to extract high-purity octa-galact-uronide from pectin.2 cl, 6 ex

ethod of producing chitosan iodo-derivatives // 2575784
FIELD: chemistry.SUBSTANCE: method includes modifying a chitosan-containing substance at room temperature in iodine-containing vapour for more than 5 hours until the required physical and chemical properties of chitosan iodo-derivatives are obtained, and the chitosan-containing substance used is a film of high-molecular chitosan with = 200 kDa in a salt or basic form, or acid-soluble high-molecular chitosan powder with = 200 kDa, or water-soluble low-molecular chitosan powder with = 38 kDa, wherein the iodine-containing vapour is formed by crystalline iodine, aqueous iodine solution or water-alcohol solution of iodine.EFFECT: method simplifies the technique of producing iodine-containing chitosan derivatives in powder form, widens the range of modification media and iodine-containing preparations for eliminating iodine-deficient conditions and treating delicate tissue, and improves stability of the end product.4 cl, 25 ex, 1 tbl

Polysaccharide and its derivatives with activity to ficolin-3, method for obtaining and application thereof // 2575752
FIELD: chemistry.SUBSTANCE: polysaccharide possesses affinity to ficolin-3 and is characterised by presence of oligosaccharide repeating unit of general formula (I). In formula (I) n is integer number from 0 to 100, preferably smaller than 50; PDt stands for oligosaccharide repeating unitPD1t of formula (II),oligosaccharide repeating unit PD2t of formula (III) or oligosaccharide repeating unit PD3t of formula , (IV),PD stands for oligosaccharide repeating unit PD1 of formula ,(V),oligosaccharide repeating unit PD2 of formula ,(VI),or oligosaccharide repeating unit PD3 of formula , (VII) [Hep]-Kdo stands for oligosaccharide repeating unit of formula (VIII).Method for obtaining claimed polysaccharide includes cultivation of H. alvei strain and separation of bacterial lipopolysaccharide. Obtained lipopolysaccharide is cleaved and polysaccharide fraction is separated. Polysaccharide fraction, containing polysaccharides, formed of repeating units with molecular weight in the range from 2400 to 26000 kDa, is separated. Obtained polysaccharides are optionally reduced, in particular in presence of NaBH4, and preferably conjugated with known carrier-protein or chromatographic medium.EFFECT: invention makes it possible to obtain ligands, which specifically find with ficolin-3.14 cl, 10 dwg, 3 tbl, 13 ex

Anionic polysaccharides, functionalised by at least two hydrophobic groups, bound with at least trivalent intermediate grouping // 2575460
FIELD: chemistry.SUBSTANCE: invention relates to novel derivatives of anionic polysaccharides, partly functionalised by at least two vicinal hydrophobic groups, with said hydrophobic groups, being similar or different, being connected with at least trivalent radical or intermediate grouping. Invention also relates to methods for their synthesis.EFFECT: invention relates to application of polysaccharides, functionalised in accordance with claimed invention, for stabilisation of protein active substances, where active substance is selected from the group, including proteins, glycoproteins, peptides and non-peptide therapeutic compounds.21 cl,15 ex

ethods for producing microbial polymer containing fucosa, polymer and uses thereof // 2573574
FIELD: medicine, pharmaceutics.SUBSTANCE: group of inventions refers to biotechnology. There are presented methods for producing a polymer containing fucosa, the same polymer containing fucosa and uses thereof. The polymer is produced by Enterobacter DSM 23139 bacterial culture in one version with using glycerol or glycerol-rich mixtures as a carbon source. According to another version, the carbon source represents food or industrial waste containing one or more compounds, such as sugar, alcohol, organic acid, alkane. During the charge phase, the bacteria are cultured in a bioreactor, mixed and aerated in a medium containing the carbon source including glycerol, a nitrogen source and non-organic salts. The feeding phase involves culturing the bacteria with the carbon source, and in the nitrogen-free environment. The polymer is applicable as an anti-cancer or anti-inflammatory agent, as a moisturising or anti-age additive, as well as for producing biodegradable composite films or microspheres for controlled released of medicinal products.EFFECT: produced polymer has the flocculating and emulsifying activity and is applicable as a gelling material.25 cl, 3 dwg, 4 tbl, 4 ex
ethod for obtaining heptagalacturonide // 2570709
FIELD: chemistry.SUBSTANCE: method of heptagalacturonide obtaining includes acid pectin hydrolysis with the following separation of liquid phase from insoluble pectin residue and extraction of heptagalacturonide from it. As pectin applied is low-etherified pectin with degree of etherification not higher than 30%. Acid hydrolysis is carried out in 0.1-2.0 mole/l in solution of mineral acid at temperature 70-100°C until concentration of oligogalacturonide in each phase not less than 0.25 mg/ml is achieved. To separate heptagalacturonide from liquid phase it is neutralised to pH not lower than 4.0. Then ethanol is added with constant mixing to concentration 12-19 vol %, precipitated sediment is removed, and ethanol is added to liquid phase with constant mixing to concentration 21-28 vol %. After that heptagalacturonide sediment is separated from liquid phase and dried. To obtain product of higher purity before drying sediment of heptagalacturonide is washed with solution, which contains 0.1-0.5 mole/l of sodium chloride and 21-28 vol % of ethanol, and then washed with 70-85 vol % of ethanol solution.EFFECT: invention makes it possible to separate heptagalacturonide with high degree of purity from pectin.2 cl, 8 ex
ethod for obtaining octagalacturonide // 2570708
FIELD: chemistry.SUBSTANCE: method of obtaining octagalacturonide includes acid hydrolysis of pectin with the following separation of liquid phase from insoluble pectin residue and separation of octagalacturonide from it. As pectin, low-etherified pectin with degree of etherification not higher than 30% is applied. Acid hydrolysis is carried out in 0.1-0.2 mole/l solution of mineral acid at temperature 70-100°C until concentration of liquid phase not lower than 0.25 mg/ml is obtained. To separate octagalacturonide from liquid phase it is neutralised to pH not lower than 4.0. Then ethanol is added with constant mixing to concentration 12-19 vol %, after that, sediment of octagalacturonide is separated from liquid phase and dried. To obtain product of higher purity before drying sediment of octagalacturonide is washed with solution, which contains 0.1-0.5 mole/l of sodium chloride and 12-19 vol % of ethanol, and then washed with 70-85 vol % solution of ethanol.EFFECT: invention makes it possible to obtain octagalacturonide with high degree of purity from pectin.2 cl, 8 ex

ethod of producing glycosides of acrylate derivatives using polysaccharides and glycosidases or glycosyltransferases // 2570556
FIELD: chemistry.SUBSTANCE: invention relates to biotechnology. Disclosed is a method of producing an ethylenically unsaturated glycoside of formula The method includes reacting an ethylenically unsaturated compound of formula with a polysaccharide containing 10 to about 200000 monosaccharide links, such as starch, amylose, amylopectin, cellulose, in the presence of a glycosidase, such as amylase, cellulase, glucosidase and galactosidase, or a glycosyltransferase, such as cyclomaltodextrin glucanotransferase.EFFECT: high purity of the product.7 cl, 3 ex
 
2551381.
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