Preparation of polysaccharides not provided for in groups and c08b0001000000-c08b0035000000 and derivatives thereof (C08B37)
C08B37 Preparation of polysaccharides not provided for in groups ; c08b0001000000-c08b0035000000; derivatives thereof (cellulose d21)(796)
FIELD: pharmacology.SUBSTANCE: conjugate of the invention is a compound of formula (C6O4H9NH2)m(C6O4H9NHX)n, wherein the remainder of the polyethylene glycol derivative, which is a monomethoxy-PEG succinate acts as a substituent on the amino group X, the molecular weight of chitosan used to prepare the conjugate is 15 to 120,000 daltons, am and n are the number of units in the molecule, where m/n is 6 to 19. The proposed method involves preparation of a solution of N-hydroxysuccinimide ester of PEG-hemisuccinate in dimethylformamide or dimethylsulfoxide, mixing of the solution prepared with an acidic solution of chitosan with a molecular weight of 15-120 kDa, and adjustment of the resulting mixture to a pH of 7.5-8.0, followed by purification by dialysis against buffer solution.EFFECT: new substance effective for liposomal suspensions stabilization is disclosed, and an effective method for its preparation.3 cl, 3 dwg, 4 ex
FIELD: biotechnology.SUBSTANCE: application of physiological lipopolysaccharide produced by the strain of the phototrophic bacterium Rhodobacter capsulatus VKM IBPM RAS B-2381D as a non-toxic factor enhancing the differentiating activity of 1α,25-dihydroxyvitamin D3 when monocytic-like cells are differentiated into monocytes, is proposed.EFFECT: invention can be used to further differentiate promonocytes and monocytes in clinical and experimental medicine and in development of drugs, in particular for oncology.2 dwg, 1 tbl
FIELD: agriculture.SUBSTANCE: method involves providing a carbohydrate-rich parenchyma tissue of a plant containing an apoplast at an agricultural crop temperature of 5 to 40°C. The said parenchyma tissue of the plant is combined with an aqueous solution containing yeast at a solution temperature of from 20 to 40°C. The said parenchymal plant tissue is influenced by the pressure of gaseous phase during preparation during the preparation time, or before the plant tissue is combined with the aqueous solution containing yeast, or after said combination. Wherein, the specified time of preparation varies from 1 minute up to 1 hour, but the pressure of gaseous phase in the preparation is less than the atmospheric pressure and ranges from 105 to 200% of the equilibrium vapour pressure at higher temperatures from the specified temperature of crop and the specified solution temperature. The said parenchymal plant tissue is influenced by the pressure of gaseous phase during infusions over time of infusions of from 1 minute up to 1 hour, where the specified yeast are infused into specified apoplast and where the specified pressure of gaseous phase at infusion is more than specified pressure of gaseous phase in the preparation. Free fluid is diverted, that was not drawn into the apoplast, then the pressure of the gaseous phase is supported at fermentation during fermentation time to obtain products of fermentation within the specified apoplast without re-entering the specified water solution containing yeast, in the specified parenchymal plant tissue. Wherein, the specified pressure of gaseous phase at a fermentation is greater than the specified pressure of gaseous phase at the preparation, specified fermentation time is from 6 hours up to 7 days, and the fermentation products are obtained by fermentation of carbohydrates, selected from a group consisting of simple sugars simple sugars obtained by hydrolysis of starch, and their combinations, and where carbon dioxide does not displace specified yeast from the specified apoplast during the fermentation.EFFECT: invention provides efficient fermentation of carbohydrate-rich crops.13 cl, 1 dwg, 5 tbl, 5 ex
FIELD: cosmetology.SUBSTANCE: invention is a process for preparation of a composition for use as a dermatological excipient in cosmetic and medical applications in the form of a gel comprising a crosslinked first polymer, optionally a second polymer that can be crosslinked or uncross linked, and water, wherein the first and second polymers are selected from polysaccharide, and the process comprises at least steps (i), (ii) and (iv), and optionally step (iii), wherein step (i) is to crosslink a mixture including the first polymer and water, step (ii) is to finish the crosslinking of step (i), step (iii) is to optionally blend the product obtained in step (ii) with the second polymer, step (iv) consists in dialysis of the product obtained in step (ii) or in step (iii), wherein the dialysis step (iv) comprises steps (iv.1)-(iv.3)(iv.1) which is extrusion of the product, obtained in step (ii) or (iii) through the first sieve and then extrusion of the product extruded through the first sieve through the second sieve, in which the second sieve hole size is smaller than the first sieve hole size; or extrusion of the product, obtained in step (ii) or (iii) through the first sieve and then extrusion of the product extruded through the first sieve through the second sieve, and then extrusion of the product extruded through the second sieve through the third sieve, in which the second sieve hole size is smaller than the first sieve hole size, and the third sieve hole size is smaller than the second sieve hole size, where step (iv.2) is to fill the dialysis membrane with the product obtained in step (iv.1), step (iv.3) is to process the filled membrane obtained in step (iv.2) with dialysis solution.EFFECT: obtaining of exclusively homogeneous compositions.14 cl, 14 ex
FIELD: chemistry.SUBSTANCE: method involves obtaining a substrate of hyaluronic acid dissolved in the first liquid medium, which is an aqueous solution, without any stitching, the deposition substrate of hyaluronic acid, subjecting it to the influence of the second liquid medium containing one or more first water-soluble organic solvent in an amount to provide the conditions for the deposition of hyaluronic acid without any stitching. In these steps, the desired shape in the form of particles, fibres, a tape, a filament, a mesh, a film, a disc or granules is imparted to the hyaluronic acid substrate. Then the precipitated substrate of non-cross-linked hyaluronic acid in the desired form is subjected to the single-stage of cross-linking in the third liquid medium under the conditions suitable for obtaining a moulded product precipitated from cross-linked hyaluronic acid. The third liquid medium has a pH of 11.5 or higher and contains one or more polyfunctional cross-linking agents and a quantity of one or more second organic solvents to ensure conditions of hyaluronic acid deposition. Moulded products from cross-linked hyaluronic acid, an aqueous composition containing one of these products and the use of a moulded product or aqueous composition in a cosmetic surgical procedure are also provided.EFFECT: invention allows to obtain a moulded product from cross-linked hyaluronic acid with a high resistance to deformation.31 cl, 6 dwg, 11 tbl, 13 ex
FIELD: pharmacology.SUBSTANCE: invention relates to a method for production of hydrophobised hyaluronic acid (Formula I). R is H+ or Na+ and R1 is H or -C(=O)CxHy or -C(═O)CH=CH-het, where x is an integer ranging from 5 to 17, and y is an integer ranging from 11 to 35, and CxHy is a linear or branched, saturated or unsaturated C5-C17 chain and het is a heterocyclic or heteroaromatic group with an arbitrary content of N, S or O atoms with at least one repeating unit containing one or more R1 -C(=O)CxHy or -C(=O)CH=CH-het groups, and where n is ranging from 12 to 4000. A method for production of this hyaluronic acid derivative, a nanomicellar composition based thereon, a method for production of a nanomicellar composition and its application in pharmaceutical and cosmetic applications, as well as a method for production of its stabilized form are also proposed.EFFECT: ensured transfer of bound substances from the hydrophobic domains of nanomicelle to the cell.35 cl, 12 dwg, 1 tbl, 38 ex
FIELD: food industry.SUBSTANCE: method for producing a polysaccharide complex from flaxseed, comprising the linseed extraction in water, the mucilages separation, the extract concentration, the polysaccharide complex drying. The extraction is carried out in distilled or drinking water at a temperature of 40°C within 1 hour with stirring. The mucilages separation is performed by centrifugation or filtration through a filter of synthetic fibers, or by decantation. The extract concentration is carried out using a rotary evaporator at the temperature of 40°C. Drying the polysaccharide complex is carried out in a spray dryer or by means of drying under vacuum at 40-50°C, or freeze-drying.EFFECT: method allows to obtain a product belonging to the functional food ingredients widely used as thickeners, emulsifiers and stabilisers.3 tbl, 4 ex
FIELD: esthetic medicine.SUBSTANCE: group of inventions relates to a skin filler composition for treatment of wrinkles on the skin, containing hyaluronic acid cross-linked with a diamine crosslinker selected from lysine or a lysine methyl ester and a carbodiimide binder; to a method for preparation of the said composition, according to which cross-linking of hyaluronic acid with a diamine cross-linker selected from lysine or a lysine methyl ester is carried out in the presence of a carbodiimide binder; as well as a method of treatment of fine lines on the skin, comprising composition administration to the dermal region of a patient.EFFECT: group of inventions provides an injectable skin filler composition that does not lead to blueing after introduction into the skin region of the patient, is not cytotoxic, has good mechanical properties and improved biocompatibility.11 cl, 18 ex, 6 tbl, 2 dwg
FIELD: chemistry.SUBSTANCE: method involves treating the feedstock with an aqueous formalin solution, holding in hydrochloric acid acidified water, extracting with an aqueous solution of ammonium oxalate, filtering and concentrating the extract on an ultrafiltration column with simultaneous dialysis, lyophilic drying of the desired product. Vegetable feedstock is pre-soaked in water at room temperature for 20-24 hours. Holding in hydrochloric acid acidified water is carried out at the pH of 0.8-3.5 and the temperature of 45-52°C for 3-4 hours. And as a feedstock, dried upground part of Sosnovsky hogweed or Siberian hogweed is used.EFFECT: invention allows to obtain pectin with an increased yield of the desired product.1 tbl, 3 ex
FIELD: chemistry.SUBSTANCE: invention relates to the method for the producing a polysaccharide derivative that comprises: (a) converting, at least, one polysaccharide having a crystallinity (CI) of, at least, 20% measured by the XRD method to contact with, at least, one compound at the temperature of not more than 70°C; and (b) subsequently bringing the product produced in step (a) into contact with, at least, one aromatic isocyanate. The aromatic isocyanate is a polyisocyanate selected from the group consisting of: methylenediphenyl diisocyanate represented by its 2,4'-, 2,2'- and 4,4'-isomers, as well as mixtures thereof, mixtures of methylenediphenyl diisocyanates and their oligomers, or their derivatives having urethane, isocyanurate, allophonate, biuret, uretonimine, uretdione and/or iminooxadiazinedione groups, as well as mixtures thereof, to produce a polysaccharide derivative having a crystallinity index of, at least, 50% of the relevant value of, at least, one polysaccharide.EFFECT: increasing the yield.15 cl, 2 dwg, 2 tbl
FIELD: chemistry.SUBSTANCE: method of producing arginine of the sulfated arabinogalactan derivative involves the reaction of the acidic form of arabinogalactan sulfate in a solution of butanol with arginine dissolved in 70% ethanol at pH 8 of the reaction solution.EFFECT: new derivative of arabinogalactan, simplification of the method of producing its derivatives.1 ex
FIELD: food industry.SUBSTANCE: method for extracting pectin substances includes washing the raw materials with water, grinding, sonication, hydrolysis and extraction, precipitation of pectin substances and their purification from the pectin-containing extract with ethyl alcohol. The sonication is carried out at the fixed frequency of 350 Hz, with the subsequent hydrolysis and extraction at the temperature of 55°C with the 1% solution of ammonium oxalate for 45-50 minutes. The obtained pectin-containing extract is purified and pectin substances are precipitated with ethyl alcohol with the addition of sodium chloride salt in the amount not exceeding 10 g per litre of the alcohol. Wherein the sonication is carried out for 20 minutes at the temperature of 40°C.EFFECT: invention allows to increase the yield of pectin and does not require the use of strong mineral acids as an extractant.3 ex
FIELD: chemistry.SUBSTANCE: method for producing low-molecular chitosan and chitosan oligomers by a chemical depolymerization method, including hydrolysis of chitosan in the presence of an acid, followed by filtration, fractionation, purification and drying of products, characterized in the fact that hydrolysis of chitosan is carried out with 2.5-12.5% dilute nitric acid at a temperature of 70°C, followed by separation of the hydrolyzate into two fractions - a precipitate of low-molecular chitosan and a mother liquor, then chitosan oligomers are precipitated from the mother liquor during addition of isopropyl alcohol and cooling. Then, both products are washed with isopropyl alcohol and air-dried, finally, the products of chitosan depolymerization are redissolved in water and dried lyophilically.EFFECT: method is proposed for producing a product of a narrow fractional composition which is highly soluble in water.5 ex
FIELD: chemistry.SUBSTANCE: method involves dispersing in water of high molecular weight chitosan with a molecular weight of 190-250 kDa with vigorous stirring, subsequent treatment in a 9.00-17.00% aqueous solution of sulfuric acid at 100-120°C for 20-55 minutes for homogeneous hydrolysis. The hydrolysis product is then precipitated from the sulfuric acid solution by introducing a 10% sodium hydroxide solution into the hot solution at a volume ratio of sodium hydroxide to sulfuric acid of 1:0.667. The product obtained is washed and dried at room temperature.EFFECT: invention makes it possible to obtain low molecular weight chitosan with a molecular weight of 5-20 kDa with a high degree of crystallinity, thermal stability, antimicrobial activity and biological compatibility.1 tbl, 3 ex
FIELD: biotechnology.SUBSTANCE: method for production of serogroup X Neisseria meningitidis capsular polysaccharide, its conjugate with a carrier protein and an immunogenic composition based on the conjugate for preparation of a vaccine against meningitis. The method is implemented using a fermentation medium comprising casaminoacid, an optimal strategy of nutrient solution addition and an improved process for polysaccharide isolation and purification, exclusive of any chromatographic methods. The polysaccharide and protein ratio in the conjugate is in the range of 0.2 to 0.6. Inventions allow to obtain purified serogroup X Neisseria meningitidis polysaccharide in a yield of 300 to 550 mg/l, average molecular weight of 400 to 550 kDa, yield of 60% to 65% at the purification stage and containing less than 0.5% of protein, less than 0.5% of nucleic acids and less than 5 EU/mg of endotoxins. X polysaccharide is adjusted to a size of 150 to 200 kDa and conjugated to a carrier protein.EFFECT: increased efficiency.9 cl, 4 dwg, 11 tbl
FIELD: food industry.SUBSTANCE: aqueous extract of polysaccharides is obtained by extraction under the influence of mechanical oscillations with the frequency of 10-20 Hz and the amplitude of 3-6 mm within 14-16 minutes. The extract is then cleaned and dried.EFFECT: invention allows to intensify the process of flax polysaccharides extraction, to improve the product quality, to reduce energy costs, to shorten the process duration, to improve the working conditions in comparison with the known production technology.1 cl
FIELD: food industry.SUBSTANCE: method comprises soaking and washing the raw material, cutting, drying, and grinding. The cut raw material is dried by blotting the surface moisture and subjected to infrared drying to the humidity of 10-12%, with the subsequent holding for 4-6 hours. The dried raw material is ground to the particle size of less than 100 mcm, and the resulting powder is re-dried to the humidity of 5-10%. Then it is divided into fractions according to the particle size: less than 15 microns - the first one, 15-25 microns - the second, and 25-100 microns - the third. The second fraction is dried to the humidity of 5-6% and subjected to re-grinding, followed by selecting the particles of less than 15 microns that are combined with the first fraction and used as food inulin. The residues of the second fraction are combined with the third fraction and used for producing ultra-pure inulin by preparing the aqueous suspension, holding it for at least 1 hour, and centrifuging. From inulin separated from membranes, suspension is prepared. The resulting suspension is cleaned from proteins with solutions of sodium chloride and sodium hydroxide, with the subsequent centrifugation, filtration and depigmentation of the obtained extract on anionite. Fructans are then concentrated and precipitated from the pre-heated concentrate with a solvent, preferably ethyl alcohol, taken in a volume equal to 1.5-2.5 of the concentrate volume, with the subsequent filtration, washing with ethanol and vacuum drying of the finished product.EFFECT: invention allows to obtain inulin by the dry method and to improve the efficiency of complex processing of inulin-containing raw materials.6 cl, 3 dwg
FIELD: chemistry.SUBSTANCE: in the method of the invention, xylan sulfate is obtained by sulfating xylan with sulfamic acid in N,N-dimethylformamide. In N,N-dimethylformamide, with stirring, sulfamic acid, urea, xylan are added and kept with stirring at a temperature of 100°C for 0.5-6.0 h. The resulting reaction mass is neutralised with a 3% aqueous solution of sodium hydroxide and concentrated in vacuo until the solvent is completely removed. Then, the dry residue is dissolved in distilled water and filtered. The obtained filtrate is purified by dialysis. The aqueous solution of the sodium salt of xylan sulfate is concentrated in vacuo until the water is completely removed.EFFECT: increased method efficiency.7 ex, 1 tbl
FIELD: chemistry.SUBSTANCE: method for sulfating xylan birch wood with chlorosulfonic acid in an organic solvent is characterized in that the sulfation is carried out in 1,4-dioxane at a temperature of 20-40°C for 3-6 hours, and xylan sulfate is isolated after neutralisation of the reaction mass with a 3% aqueous solution of sodium hydroxide, followed by purification by dialysis.EFFECT: effective way of sulphating xylan.1 tbl, 9 ex
FIELD: food industry.SUBSTANCE: invention refers to food and medical industry. The proposed method implies that arabinogalactan is extracted during 30-40 minutes in an ultrasonic device with frequency 27-42 kHz at a temperature of 20-30°C with water duty of 1.3-7 to the dry weight of the raw material, then the extract is filtered, dispersed by ultrasonic spraying and concentrated in a receving tank up to dry matter concentration within 20-40%. The concentrate is precipitated by the addition of organic water-mixed solvent at the ratio 1:4. The precipitated arabinogalactan is washed in the same solvent, dried and ground. It is preferable to extract arabinogalactan without pre-extraction of dihydroquercetin. The arabinogalactan extract is condensed in serially connected vessels to obtain arabinogalactan with specified molecular weight. After arabinogalactan precipitation and washing, the extraction agent is filtered, distilled and reintroduced to the arabinogalactan manufacturing process.EFFECT: invention allows for the production of arabinogalactan of high purity with specified molecular weight and additional presence of dihydroquercetin, reduction of power inputs and of extraction process duration.4 cl, 1 dwg, 2 tbl, 3 ex
FIELD: food industry.SUBSTANCE: method of obtaining inulin from chicory and elecampane is proposed, including preparing the inulin-containing raw material, its mechanical cleaning, washing the roots, rhizomes, stems, their grinding and mixing. The mixed and shredded pieces of raw material are extracted twice with hot water at the temperature of 75°C for 2-3 days at constant stirring. The resulting inulin extract in mass is treated with 96% ethanol at a ratio of 1:1 by volume, with the subsequent deposition of inulin at the temperature of minus 16°C. The resulting inulin is intended for use as a food additive in manufacturing functional meat, flour and dairy products.EFFECT: quality improvement.1 ex
FIELD: food industry.SUBSTANCE: method for producing a pectin-containing product from flax grain includes moistening flax grain, its softening and treatment by IR-rays. Moistening the flax grain to the humidity of 16-18% is carried out at the temperature of 18-20°C and softening durability of 6-8 hours. The treatment with IR rays is carried out at the wavelength of 0.9-1.1 mcm and the radiant flux density of 20-22 kW/m2 within 70-90 seconds before the grain reaches the temperature of 150-160°C and the final humidity of 7-8%.EFFECT: enhanced quality and high biological value.5 ex
FIELD: pharmacology.SUBSTANCE: method involves hydrogen peroxide addition to dextran aqueous solution to a final concentration of 0.25 to 5.0% in the solution, depending on the desired depth of dextran oxidation. The mixture is stirred, poured into a container with a flat inert surface and distributed in a thin layer. Simultaneous oxidation of dextran and drying of the desired product is performed at 90-95°C for 1-2 hours. Preferably, a 10% aqueous dextran solution with a molecular weight of 40 kDa or 70 kDa is used. Pallets made of glass, aluminium alloys, stainless steel, with ceramic or Teflon coating are used as an inert surface. Preferably, mixture layer thickness before oxidation and desired product drying is 1-3 mm.EFFECT: improved method.4 cl, 2 tbl, 3 ex
FIELD: pharmacy.SUBSTANCE: invention refers to chemically modified glycosaminoglycan, which is heparin or heparan sulfate with anti-factor IIa activity less than 10 IU/mg, anti-factor Xa activity less than 10 IU/mg and average molecular weight from 4.6 to 6.9 kDa.EFFECT: invention also refers to a method for production of modified glycosaminoglycan and its medical application.45 cl, 3 dwg, 4 tbl
FIELD: agriculture.SUBSTANCE: chitosan production method is proposed, comprising grinding of insect puparia, alkaline treatment of chitin-containing raw materials with constant strirring at elevated temperature and further washing of the remainder with distilled water. Musca domestica puparia are used. Alkaline treatment is carried out at a temperature of 80°C with 1-1.5% alkali. Two-stage treatment is additionally performed with 3.5-5% hydrochloric acid and 4.5-5% sodium hydroxide while stirring for 1.5-2 hours at each stage, followed by filtration and washing of dry extract obtained after each processing stage with distilled water. Puparia are pre-washed with water from foreign substances before grinding.EFFECT: increased finished product output.2 tbl, 3 ex
FIELD: pharmaceutics.SUBSTANCE: group of inventions relates to pharmaceutics and provides a process for producing sulfoalkyl ethers of cyclodextrin ensuring a low content of the components, degrading the drug in particular the chlorides due the stage of processing by activated carbon with a conductivity of 10 µs or less, as well as to the product, obtained using said process and composition for delivery of active agents sensitive to the content of chlorides.EFFECT: group of inventions allows to reproducibly obtain sulfoalkyl esters of cyclodextrin with reduced chloride content and provides improved stability of the composition with active agents that are sensitive to chloride content.33 cl, 10 dwg, 13 tbl, 21 ex
FIELD: chemistry.SUBSTANCE: method of producing heparin involves thawing and homogenisation of raw material, filling with 0.6M sodium chloride solution, adding alcalase to a final concentration of 0.1-0.5%. Pigs' frozen mucous membrane of the small intestine (mucosa) with a shelf life of no more than 6 months at -20°C is used as raw material. Enzymatic hydrolysis is being performed at 65°C and pH 8.5-8.6 for 6 hours, then the enzyme is being inactivated at 90°C for 15 minutes. The produced hydrolyzate is cooled to temperature of 15-20°C and centrifuged. Concentrated hydrochloric acid is added to supernatant fluid to pH 3.0, heated to 70°C and being held for 30 minutes for additional hydrolyzate decontamination of protein origin ballast substances. The resulting residue is removed by way of centrifugation. Thereafter, end product sorption is performed in dynamic mode from the supernatant fluid on anion exchanger YMC-BioPro Q75. Anion exchanger is pre-equilibrated by 0.6M sodium chloride solution. After the sorption is complteted the anion exchanger is washed with 0.6M sodium chloride solution, ballast substances are removed from the anion exchanger with 0.9 M sodium chloride solution, heparin is desorbed from the anion exchanger with1.4 M sodium chloride solution.EFFECT: invention increases output of heparin, its specific activity, reduces the technology process.1 cl, 5 ex
FIELD: food industry.SUBSTANCE: invention relates to food, pharmaceutical and biochemical industries. Method of pectin production includes extraction of pectin-containing pomaces by solution of food or mineral acid with pH of 0.8-3.2 and hydraulic module of 1:(15-45) for 1-6 hours at temperature of 70-95°C, filtration of produced extract, its treatment. Then extract is concentrated on a cascade of ion-exchange and adsorption columns, ultrafiltration and diafiltration plants with polymer membranes with cut off at 20-100 kDa. Produced pectin solution is dried at temperature of 45-95°C to produce pectin in drying belt vacuum deviced with subsequent grinding of pectin or devices of spray type. Besides, removal of ballast substances in ion exchange and adsorption processes is carried out on cation exchange resins AMBERLITE® FPC23 H, anion exchange resins AMBERLITE® FPA90 Cl and adsorption resins AMBERLITE® FPX66.EFFECT: invention makes it possible to increase yield of pectin, extent of purity and to produce pectin with specified molecular mass.7 cl
FIELD: food industry.SUBSTANCE: invention relates to polysaccharides rich composition containing beta-glucan, chitin and chitosan, extracted from Saccharomyces cerevisiae cell wall from biomass, which is brewing process by-product. A method for preparing the composition involves the following stages: reactor preparation with NaOH solution with concentration from 0.25 to 3 M while stirring and temperature from 50 to 95 °C, addition biomass produced during brewing to said solution, maintenance of said conditions for at least 1 hour. Then, said solution is cooled down to room temperature, solution neutralization by at least single adding of acidic solution or water until pH 7. At that, in case of more than single adding stage of solid product separation from said solution is carried out between additions. Then solid product transfer to at least single washing with water is carried out and separation of obtained solid product, solid product drying to constant mass and fine grinding. Obtained composition can be prepared in form of edible, pharmaceutical or veterinary product.EFFECT: invention enables to obtain composition, which is capable of selective fats binding, and therefore it can be used for preventing and/or treating diseases, such as overweight, obesity, hypercholesterolemia, hypertriglyceridemia, hypertension and cardiovascular disorders.13 cl, 5 dwg, 18 tbl, 9 ex
FIELD: chemistry.SUBSTANCE: method comprises exhaustive extraction of starting material with water, separation of plant material, deposition of water-soluble polysaccharides, washing and drying. Starting material used is common burdock roots ground to linear size of 0.2-0.5 mm . Extraction is three-fold with hot purified water in ratio of raw material and extractant of 1:30 when processing in ultrasonic bath with frequency 35 kHz for 30 minutes at a temperature of 80 °C. Plant material is separated by filtration, and water-soluble polysaccharides are deposited three-fold amount of 95 % ethanol while stirring, cooling in freezing chamber at temperature -18 °C for 1 hour. Precipitate is filtered through a dry ash-free paper filter under vacuum at residual pressure of 0.4-0.8 atm. Filter cake is washed successively with a solution of 95 % ethyl alcohol in purified water (3:1) with ratio of raw material and solution of 1:15 and mixture of ethyl acetate and 95% ethyl alcohol (1:1) at a ratio of raw material and solution of 1:10. Filter with precipitate is then dried at a temperature of 100-105 °C to constant weight.EFFECT: invention reduces duration of extraction and increases output of water-soluble polysaccharides.1 cl, 3 dwg, 2 ex
FIELD: food industry.SUBSTANCE: invention relates to food industry. Disclosed is a method for producing agar, including its extraction from algae by ionic liquid, represented by the deep eutectic with melting point which is lower than 100 °C. At the end of the extraction process, the produced solution is being separated into algae residue and the agar solution in the deep eutectic, and alcohol is being added in the latter. Then, the hot solution is being filtered and separated into agar and alcohol-containing solution of the deep eutectic, from which the latter is regenerated and reused. Alga Ahnfeltia is used as an alga, preliminary cleaned from impurities, by electrohydraulic treatment in dilute alkali solution, then, inner steam destruction of algae thallomes is being performed, then, treated algae is being extracted by the deep eutectic. Alcohol, deep eutectic and water are being regenerated and reused, besides, the agar, produced by filtering, is being dehydrated.EFFECT: invention ensures producing high-purity agar, and the process duration is considerably reduced.9 cl
FIELD: food industry.SUBSTANCE: invention relates to food industry. Method of producing water-soluble oligosaccharides from hardwood shavings involves extraction of oligosaccharides at temperature of 170 °C, separation by filtration from shavings and centrifugation. Extraction of vegetable raw materials is carried out with treated hot water for 120 minutes, with subsequent separation on a chromatograph, to produce end product. Extraction is performed from birch or aspen shavings, with ratio of water to shavings of 1:30, method includes performing repeated extraction at ratio of water to shavings of 1:10, and separation, to output of end product, is carried out using gel-penetrating chromatograph.EFFECT: method increases output of water-soluble oligosaccharides.1 cl
FIELD: chemistry.SUBSTANCE: method comprises providing carbohydrate-rich substrate and aqueous solution with hydrolysis catalyst. Evacuation pressure is maintained above substrate for introduction of hydrolysis catalyst into carbohydrate-rich substrate to produce reaction mixture. Said reaction mixture is exposed to high pressure of gas phase at step of hydrolysis reaction, reaction mixture is held to produce monomers and/or polymers of sugars in open space inside fibres of substrate. Reaction mixture is exposed to pressure of gas phase, which is lower than pressure of hydrolysis reaction, for removing said monomers and/or polymers of sugars from said open space inside fibres of substrate into said aqueous solution and held. Steps of hydrolysis and extraction are repeated in at least two cycles of changing pressure to extract maximum amount of monomers and/or polymers of sugars from open space inside fibres of substrate. Monomers and/or polymers of sugars are then separated from aqueous solution. Method does not include treatment with water vapour or breakdown with ammonia of fibres of carbohydrate-rich substrate.EFFECT: invention simplifies method of extracting sugars from carbohydrate-rich substrates.25 cl, 1 dwg, 5 tbl, 7 ex
FIELD: food industry.SUBSTANCE: present invention can be used for producing a natural chitosan biopolymer from waste generated by industrial processing of shellfish. Shell-containing raw material of shellfish is preliminarily mixed with water in the ratio of 1:15 and milled with simultaneous deproteinization by electrohydraulic shocks created by ultra-long discharges. Demineralisation of the precipitate is performed with a hydrochloric acid solution with the volume ratio of 2-4 % at the water duty of 1:10 and the temperature of 20-25 °C and continuous mixing for two hours. Precipitate is separated from the supernatant, washed to pH 7.0, then the obtained chitin is soaked in the sodium hydroxide solution with the weight ratio of 35-45 % for 1-2 hours at 95-98 °C.EFFECT: invention ensures a shorter process of chitosan production.1 cl, 1 dwg, 1 tbl, 2 ex
FIELD: medicine.SUBSTANCE: invention relates to pharmacology, and describes a method of producing coordination compounds of oligogalacturonic acid with biogenic metals (II). Method involves production of pectin-containing preparation of oligogalacturonic acid with polymerisation degree equal to no less than 10, subjected to reaction with metal salt (II): copper, cobalt, iron, zinc, manganese, magnesium in aqueous medium at neutral pH, exposing reaction mixture at room temperature, neutralisation of formed acid, water flushing, centrifugation and drying precipitate. According to invention to 1 l of 5.0·10-3 M aqueous solution of purified oligogalacturonic acid while stirring is added first 2 M aqueous solution of ammonia hydrate to pH 8, then 1 l of 4.5·10-2 M aqueous solution of metal acetate (II); formed gel-like precipitate is treated with 96 % ethanol at volume ratio of 1:2, held for 2 hours.EFFECT: invention ensures delivery of biogenic metals (II), as well as removal of heavy metal cations (II) from body.1 cl, 8 tbl, 4 dwg, 6 ex
FIELD: chemistry.SUBSTANCE: complex esters of hyaluronic acid and hydroxycinnamic acid derivatives are proposed. With that, hydroxycinnamic acid derivatives are selected from ferulic acid and caffeic acid. Method of producing esters of hyaluronic acid involves dissolving sodium salt of hyaluronic acid in formamide. With that, the said salt is introduced into the reaction in the presence of an equivalent amount of tertiary hydroxycinnamic acid derivatives with base pre-activated carbonyldiimidazole at the room temperature. Obtained viscous mass is diluted with water solution of NaCl, the reaction product is extracted by deposition of acetone followed by purification of methanol, filtration and drying in vacuum.EFFECT: invention allows obtaining esters of hyaluronic acid and hydroxycinnamic acid derivatives used for skin protection as the compounds that reinforce elasticity with moisturizing, softening action.15 cl, 6 dwg, 1 tbl, 9 ex
FIELD: biotechnology.SUBSTANCE: group of inventions relates to biotechnology. Disclosed are methods of producing sialylated sugar chain. Method comprises reacting a sugar chain with CMP-sialic acid in presence of ST6Gal-I and phosphatase temperature from 20 °C to 37 °C for 8-48 hours to produce triantennary or tetraantennary N-linked sialylated sugar chain, containing sialic acid on each of its non-reducing ends. In another version, before said step, method involves reaction of sugar chain with UDP sugar in presence of glycosyltransferase. In third version, method involves reaction of agalacto-biantennary complex sugar chain with UDP-GlcNAc in presence of MGAT4 and MGAT5. Obtained product reacts with UDP-Gal in presence of β4GalT1, then with CMP-sialic acid in presence of ST6Gal-I and phosphatase.EFFECT: invention enables to obtain triantennary or tetraantennaryα2,6-sialylated on each of their non-reducing ends sugar chains with high output in single-reactor synthesis.15 cl, 9 dwg
FIELD: chemistry; instrumentation.SUBSTANCE: group of inventions relates to diagnostics, specifically to a device for detecting analytes, including plastic substrate, partially or completely directly coated with binding polymers, fixed on substrate noncovalently, wherein said binding polymers contain polysaccharide frame equipped with: aromatic groups of form -X-CONH-Z, groups of carboxylic acid of form -X-COOH and reactive groups F, having form -X-CONH-Z′, where X denotes a straight or branched, substituted or unsubstituted alkyl chain containing from 1 to 6 carbon atoms, Z denotes an aryl function, Z′ denotes a group which is capable of binding with other molecule, as well as methods for production of said device, and also to binding polymer and method of its production.EFFECT: group of inventions enables to obtain a device for detecting different analytes.20 cl, 13 dwg, 34 ex
FIELD: medicine.SUBSTANCE: invention relates to medicine, particularly to a new matrix material for tissue bioengineering and regenerative medicine and methods of its production. Matrix material is designed based on vegetal polysaccharide - pectin with etherification degree of not more than 50% and proteins of intracellular matrix-collagens I and IV types, which contains said components in the following concentrations: 0.5-2.0 wt%, 0.1-1.5 wt% and 0.01-0.5 wt%, respectively. Method of production of the implanted matrix material for regenerative medicine in the form of hydrogel involves mixing at a temperature of 5 °C max of calcium acetate solution of type I collagen to final concentration of 0.1-1.5 wt% with type IV collagen solution to the final concentration 0.01-0.1 wt%, then the produced mixture is mixed with previously prepared gel formation initiator, including calcium chloride solution in the concentration, providing a gel forming process, non-toxic buffer system in amount sufficient for neutralisation of the mixture, and sodium chloride solution up to physiological concentration; then the pectin solution is introduced with degree of esterification of 50% max to the final concentration 0.5-2.0 wt% and the gel is formed by temperature rise up to physiological. Invention also relates to a method of producing implanted matrix material for regenerative medicine in the form of hydrogel, which involves mixing at a temperature of 5 °C max of calcium acetate solution of type I collagen to final concentration of 0.1-1.5 wt% with IV type collagen solution to the final concentration 0.01-0.1 wt%, then the produced mixture is neutralised, stabilised by the non-toxic buffer system and the sodium chloride is added up to physiological concentration then the pectin solution with etherification degree of not more than 50 % to the final concentration 0.5-2.0 wt% is added, to obtain a fluid composition, from which the gel is formed.EFFECT: invention enables to obtain a biocompatible implantable materials with a low rate of biodegradation and ability to stimulate regenerative process, in particular, recovery of nerve conductors after a brain injury.10 cl, 8 dwg
FIELD: chemistry.SUBSTANCE: invention relates to chemistry of compounds of chitosan dodecahydro-closo-dodecaborate B12H122−−anion, chitosan, magnesium and aluminium salts, specifically to adducts of chitosan dodecahydro-closo-dodecaborate with nitrates or perchlorates of magnesium or aluminium and a method for production thereof. Synthesised novel products may be used as energy-intensive additives of various compositions, for example pyrotechnical substances or primers. Adducts of composition (C6O4H9NH3)2B12H12×nMgA2 or (C6O4H9NH3)2B12H12×nAlA3, where 0<n<1, A−NO3− or ClO4−.EFFECT: wherein adducts have optimum properties, where n is equal to 0,18 ÷0,25.2 cl, 3 dwg, 4 ex
FIELD: medicine.SUBSTANCE: group of inventions refers to medicine and pharmaceutics, namely, to immune stimulating composition in the form of a gel having the melting point of gel transition to sol above 37 °C and containing from 0.1 to 6 % of soluble beta-glucan, which is obtained from yeast and includes the main chain of β-(1,3)-bound glucose residues and side chains containing two or more β-(1,3)-bound glucose residues, herewith the side chains are connected to the main chain with β-(1,6)-link, and in fact does not contain repeating β-(1,6)-bound glucose residues; and a gelling agent, which represents carboxymethylcellulose or gellan gum, as well as to a method for its production and use to facilitate wound or ulcer healing, to treat oral mucositis, to treat cancer and to ensure skin cells proliferation in vitro.EFFECT: group of inventions provides a synergetic effect between glucan and the gelling agent.28 cl, 5 ex, 5 tbl, 9 dwg
FIELD: technological processes; chemistry.SUBSTANCE: invention relates to method of producing low-molecular water-soluble chitin. Method provides for treatment of high-molecular chitin powder with particle size of 10-100 mcm and viscosity-average weight of 1,000 kDa. Treatment is carried out in electronic beam plasma-chemical reactor with installed inside the working chamber reactor electromagnetic system in low-temperature strongly nonequilibrium electron-beam oxygen plasma, or steam, or nitrogen for 1-60 minutes at pressure of plasma forming medium 5-10 Torr, accelerating voltage 30 kV, current electron beam 1.5-2.5 mA, temperature in reaction volume of 50-90 °C.EFFECT: invention allows to produce sterile low-molecular water-soluble chitin without formation of toxic side compounds emissions and drains, polluting environment.1 cl, 1 dwg, 12 ex
FIELD: food industry.
SUBSTANCE: invention relates to food industry. Pectin production method envisages washing before hydrolysis-extraction of milled pectin-containing raw material with drinking water for 0.5-1.5 hour with simultaneous mixing at 10-30 rpm and t = 40-60 °C, separation of the obtained mixture into solid and liquid phases, mixing the solid phase with purified water in the ratio of 1:10-1:40. Herewith the drinking water is preliminarily purified on a two-stage reverse osmosis plant. Mixture obtained after washing is heated up to 50-80°C with simultaneous stirring and circulation, hydrolysis-extraction is performed by cavitation treatment in a rotary-oscillatory apparatus with further separation of pulp into solid and liquid phases in a vacuum drum filter. Obtained liquid phase is centrifuged with the relative centrifugal force of FR = 2,000-5,000 g followed by microfiltration at P = 0.1-0.3 MPa and V = 0.06-0.16 l/sec, ultrafiltration at P = 0.2-0.6 MPa and V = 0.15-0.30 l/sec, diafiltration at P = 0.2-0.6 MPa and V = 0.1-0.27 l/sec till pectin substances concentration reaches 1-2 %. Herewith ultrafiltration and diafiltration are carried out on tangential filters with ceramic membranes. Then it is thickened in a vacuum-evaporator till dry substances content reaches 3-8 % and spray-dried at the air temperature of 150-200 °C.
EFFECT: output of the pectin is 70-84 % of theoretically possible one.
1 cl, 4 tbl, 3 ex
SUBSTANCE: invention refers to pharmaceutical industry and deals with inclusion complex of disulfiram (DSF) and cyclodextrin (CD), selected from a group consisting of unsubstituted cyclodextrin (uCD) or substituted cyclodextrin, such as methylcyclodextrin (MCD), dimethylcyclodextrin (DMCD), trimethylcyclodextrin (TMCD) and hydroxypropylcyclodextrin (HPCD) produced by the method, in which CD, taken in a solid or dissolved in water or alcohol form, when stirred at a temperature from 20 to 70 °C is combined with DSF in the form of a solid substance, fusion or alcohol solution, where alcohol is selected from methanol, ethanol, n-propanol or iso-propanol, where molar ratio of CD and DSF ranges from 2.01:1 to 2.40:1. Obtained mixture is cooled to 0-25 °C, subjected to sterilising filtration and lyophilized, or solvent is evaporated.
EFFECT: reception of water-soluble inclusion complex of disulfiram with cyclodextrin, which can be extracted with high degree of purity.
1 cl, 5 dwg, 2 tbl, 8 ex
FIELD: technological processes; chemistry.
SUBSTANCE: invention relates to a method of dextranal producing. Method envisages modification of dextran with molecular weight 20-75 kDa in the form of 5-25 % aqueous solution by mechano-activation processing initial dextran in apparatus with energy of activation of 16-85 kJ/mol. Preferably dextranal is extracted from its aqueous solution by ethanol and dried.
EFFECT: invention enables to obtain easily soluble derivative of dextran for pharmaceutical, cosmetic and food industries, eliminating harmful impurities and simultaneously eliminating loss of product.
1 cl, 1 tbl, 3 ex
FIELD: technological processes.
SUBSTANCE: invention relates to method of oxidised dextran producing. Method involves oxidation of aqueous solution of dextran with molecular weight of 20-75 kDa with potassium permanganate solution in medium of acetic acid, settling and filtering solution from impurities, separation of oxidised dextran with ethanol and drying. At that, initial dextran in form of 20-25 % acetic acid aqueous solution in concentration of 32-34 % in amount of 0.005-0.1 % of initial volume of dextran solution, potassium permanganate solution in concentration of 2 % in amount of 20-25 % of weight of dry dextran in solution, and ethanol is in concentration of 95-96 % with ethanol to liquid ratio of (1.4-1.6):1. Preferably dextran oxidation is carried out at temperature of 60-70 °C, filtering of oxidised dextran solution from impurities is carried out on membrane micro filter, and drying of oxidised dextran is carried out in vacuum oven at temperature of 60-85 °C.
EFFECT: invention enables to obtain oxidized dextran with higher output due to creation of conditions for maximum possible release of all fractions of oxidised dextran, not only medium and high-molecular but and low-molecular ones, eliminating loss of oxidised dextran and simultaneously increase organoleptic properties of oxidised dextran and its solubility in water.
4 cl, 3 ex
FIELD: chemistry.SUBSTANCE: invention relates to polymer materials and specifically to a method of producing granules of cross-linked chitosan, which involves cross-linking chitosan with glutaric aldehyde using hydrochloric acid solution containing glutaric aldehyde, with molar ratio of chitosan:hydrochloric acid:glutaric aldehyde, equal to 1:(0.5-1.0):(0.1-1.0), and then extrusion moulding gel in form of threads, which are mechanically cut into granules and dried at temperature 40-70 °C for 1-2 hours.EFFECT: invention provides decrease in method of amounts of acid, glutaric aldehyde and precipitation agents, enables to obtain chitosan granules of controlled size.1 cl, 6 ex
FIELD: cosmetology.SUBSTANCE: invention relates to method of producing cross-linked gel polysaccharide and use thereof to long period of filling volume of skin defects. In method of obtaining aqueous gel containing polysaccharide in crosswise-cross linked form together with bifunctional or multifunctional epoxy cross-linking agent, at temperature below 35 °C. Obtained mixture is held at temperature lower than 35 °C during at least one hour. Cross-linking reaction of prepared mixture is stimulated. Then, if necessary restored formed cross-linked gel.EFFECT: invention ensures production of gels with improved viscoelastic properties.14 cl, 3 dwg, 2 tbl, 3 ex
FIELD: pharmaceutics.SUBSTANCE: invention relates to pharmaceutical industry, specifically to fine powders of aloe and use thereof. Method of preparing fine-grained powder of aloe extract, which includes weighing freeze-dried aloe powder, where freeze-dried aloe powder has at least 25 % polysaccharides aloe; dissolution of freeze-dried aloe powder in deionised water to prepare a solution; addition of organic solvent in solution to obtain a first mixture; settling of first mixture to produce precipitate; extraction of part of supernatant fluid from first mixture and addition of organic solvent in amount exceeding volume of supernatant solution to obtain a second mixture; treatment of second mixture in a centrifuge; tracking appearance of sediment in second mixture; addition of organic solvent in first mixture, if there is formation of any sediment in second mixture; pumping supernatant fluid of first mixture, where pumping occurs only in case there is no any sediment in second mixture; filtration sediment from first mixture; extraction of powder aloe extract; placing aloe extract powder in flask for freeze-drying in freezing unit; freeze-drying frozen aloe extract powder in freeze-drying unit; and grinding freeze-dried aloe extract powder in the device for grinding to fine-grained texture, organic solvent is selected from a group of methanol, ethanol, isopropyl alcohol, propanol, under certain conditions. Use of fine-grained powder extract of aloe vera in manufacture of a medicament with immunomodulating and anti-cancer properties. Injectable composition having immunomodulating and anti-cancer properties, containing an extract of aloe vera and one or more pharmaceutical preservatives. Use of injection composition for preparing a drug for treating one or more cancer diseases, selected from a group comprising leukaemia, lymphoma, prostate cancer, breast cancer and colon cancer. Use of the injection composition for preparing a drug for immunomodulation or immune stimulation in a human with disturbed immune system or immune disease.EFFECT: aloe extract powder described above is characterised by content of polysaccharides with high molecular weight, possesses pronounced immunomodulatory and anticancer properties.37 cl, 7 dwg, 9 tbl
FIELD: chemistry.SUBSTANCE: invention relates to new derivative of hyaluronic acid of general formula (I), method for production thereof, to hydrogel based on it, to method of producing hydrogel and to use of hydrogel for producing preparations for cosmetic products, medicine or regenerative medicine. At that, Ar is phenyl and R1 is ethylene or Ar is indole and R1 is ethylene or Ar is indole and R1 represents carboxyethylene. R2 represents alkyl with 3-7 carbon atoms, and n is ranges from 1 to 7,500.EFFECT: invention enables to obtain material, sufficiently strong and elastic, not exhibiting significant changes of biological and physical properties and forming strong hydrogel.12 cl, 1 dwg, 1 tbl, 18 ex