Compounds containing only hydrogen atoms and saccharide radicals having only carbon, hydrogen, and oxygen atoms (C07H3)

C   Chemistry; metallurgy(315514)
C07   Organic chemistry(61593)
C07H3                 Compounds containing only hydrogen atoms and saccharide radicals having only carbon, hydrogen, and oxygen atoms (preparation by hydrolysis of di-or polysaccharides c13; separation or purification of sucrose, glucose, fructose, lactose or maltose c13)(138)

Glycosylated polypeptides and medicinal compositions containing these polypeptides // 2627184
FIELD: biotechnology.SUBSTANCE: glycosylated somatostatin analogs containing two or three glycosylated amino acids are obtained.EFFECT: invention allows to increase the stability of somatostatin analogues in blood in comparison with somatostatin.14 cl, 20 dwg, 10 tbl, 91 ex

Glycosylated polypeptides and medicinal compositions containing these polypeptides // 2624034
FIELD: biotechnology.SUBSTANCE: invention relates to production of glycosylated polypeptides having affinity for somatostatin receptors and can be used in medicine for somatostatin-related diseases treatment or prevention. SRIF14 and SRIF28 glycosylated polypeptides are obtained by Asn or Cys glycosylation.EFFECT: invention provides preparation of glycosylated polypeptides having an affinity for somatostatin receptors and better stability in blood compared to somatostatin.14 cl, 19 dwg, 10 tbl, 88 ex

Immunogenic compositions of multiple antigen presentation, methods and applications relating thereto // 2619176
FIELD: biotechnology.SUBSTANCE: immunogenic composition against one or more polysaccharide antigen, peptide antigen or polypeptide antigen comprises at least one antigenic polysaccharide, at least one peptide or polypeptide antigen and at least one complementary affinity molecules pair. At that, the first affinity molecule is associated with at least one antigenic polysaccharide, and the complementary affinity molecule is associated with at least one peptide or polypeptide antigen, wherein the said first affinity molecule binds to the complementary affinity molecule for the compound of peptide or polypeptide antigen and a polysaccharide antigen.EFFECT: invention allows to induce both humoral and cellular immune responses to one or multiple antigens simultaneously.20 cl, 40 dwg, 4 tbl, 4 ex

Cellulose hydrolysis with ph adjustment // 2609001
FIELD: pulp and paper industry.SUBSTANCE: present invention relates to a method of processing lignocellulose and can be used in chemical industry. Disclosed method involves preparation of lignocellulose biomass, which comprises a first solid fraction, containing cellulose and lignin, a first liquid fraction; separation of said first solid fraction from said first liquid fraction; mixing said first solid fraction with water to form a suspension; wherein said suspension has pH from 3.0 to 4.5; raising said pH of said suspension by 0.5 pH units to 5.0 pH units, to obtain a suspension with adjusted pH; wherein said suspension with adjusted pH has pH from 5.0 to 8.0; optionally, preliminary heating of said suspension with adjusted pH to temperature lower than critical point of water; bringing said suspension with adjusted pH into contact with a fluid for a second reaction, containing supercritical or close to supercritical fluid, to obtain a reaction mixture which contains a second solid fraction containing lignin; and a second liquid fraction containing soluble C6-saccharide, selected from a group consisting of cellulo-oligosaccharides, glucose, galactose, mannose, fructose and mixtures thereof; wherein said supercritical or close to critical fluid contains water and, optionally, CO2 at temperature equal to 300 °C or higher, and pressure, at least sufficiently high to ensure that all fluid for second reaction is in liquid phase or supercritical phase; and where said bringing said suspension with adjusted pH into contact with said fluid for second reaction has duration of more than 2 seconds; optionally, reducing temperature of said reaction mixture to temperature lower than 280 °C; and optionally, hydrolysis of said second liquid fraction to form C6-saccharide, selected from a group consisting of C6-oligosaccharide, having links with lower degree of polymerisation, glucose, galactose, mannose, fructose and mixtures thereof.EFFECT: proposed method enables to efficiently prepare lignocellulose biomass, control degree of hydrolysis of cellulose and reduce level of decomposition of glucose.31 cl, 3 ex, 7 dwg, 2 tbl

Enhanced soluble c5-saccharide yields // 2609000
FIELD: chemistry.SUBSTANCE: present invention relates to a lignocellulose hydrolysis method and can be used in chemical industry. Disclosed method involves providing a fractionated lignocellulose biomass containing a fraction of solid substances, containing optionally insoluble C5-oligosaccharide, cellulose and lignin, and a first liquid fraction at a first temperature of not more than 240 °C, containing soluble C5-saccharides, selected from C5-oligosaccharides, xylose, arabinose, lyxose, ribose and mixtures thereof; contacting said first liquid fraction with solid acid catalyst to form a second liquid fraction at temperature not more than 240 °C; wherein said second temperature is lower than said first temperature; where said contacting shifts molecular weight distribution of said soluble C5-oligosaccharides to lower average molecular weight; optionally hydrolysis of said second liquid fraction using acid or enzyme to produce C5-oligosaccharides, selected from C5-oligosaccharides containing fewer monomer links, xylose, arabinose, lyxose, ribose and mixtures thereof; where said fractionated lignocellulose biomass is obtained by bringing said cellulosic biomass into contact with first reaction liquid, containing hot water under pressure and optionally carbon dioxide; where said first reaction liquid additionally contains acid, where said lignocellulose biomass includes soft wood; where said first reaction liquid is at temperature less than 100 °C under pressure sufficient to maintain said first reaction liquid in liquid form.EFFECT: novel efficient lignocellulose hydrolysis method, which increases level of soluble C5-saccharides preventing reverse condensation of soluble C5-oligosaccharides to insoluble high-molecular weight C5-oligosaccharides.61 cl, 4 dwg

ultistage cellulose hydrolysis and quench with or without acid // 2608999
FIELD: pulp and paper industry.SUBSTANCE: present invention relates to methods of processing lignocellulose biomass. Proposed method comprises feeding lignocellulose biomass, including a first solid fraction of cellulose and lignin and a first liquid fraction; optionally separating said solid and liquid fractions; mixing said solid fraction with water to form pulp with preliminary heating of pulp to 210 °C–240 °C at 225–250 bar; contacting said pulp with a second reaction liquid to form a second reaction mixture, including a second solid fraction of lignin and a second liquid fraction of soluble C6 saccharide, selected from C6 monosaccharides, C6 oligosaccharides and mixtures thereof; wherein said second reaction liquid includes supercritical water and optionally carbon dioxide and is located at a temperature of at least 374.2 °C and pressure sufficient to maintain said second reaction liquid in supercritical state; lowering temperature of said pulp below 140 °C; optionally acid hydrolysis of said second liquid fraction with formation of a composition containing C6 saccharide, selected from C6 oligosaccharide, having fewer elementary links, glucose, galactose, mannose, fructose and mixtures thereof.EFFECT: novel method makes it possible to control rate of hydrolysis of cellulose, reduce rate of decomposition of glucose, increase content of C6 monosaccharides and C6 oligosaccharides.46 cl, 2 ex, 1 tbl, 2 dwg

Conjugation of staphylococcus aureus type 5 and type 8 capsular polysaccharides // 2603267
FIELD: biochemistry.SUBSTANCE: present invention relates to biochemistry, particularly to a method of producing a conjugate of S.aureus type 5 or type 8 capsular polysaccharide and a carrier protein. Method involves a step for depolymerisation of capsular polysaccharide by acid hydrolysis to obtain polysaccharide fragment, step for oxidising fragment for introduction of an aldehyde group into at least one saccharide residue in fragment to produce an oxidised saccharide residue and a step of binding oxidised saccharide residue with carrier protein through an aldehyde group. At oxidation step two vicinal hydroxyl groups of polysaccharide are converted to aldehyde. Present invention provides a conjugate of S.aurens type 5 or type 8 capsular polysaccharide and carrier protein, obtained using said method, and an immunogenic composition including said conjugate.EFFECT: present invention improves immunological properties of obtained conjugate.13 cl, 18 dwg, 8 tbl, 1 ex

Isomalto-oligosaccharide production and use thereof // 2589712
FIELD: food industry.SUBSTANCE: present invention relates to method of producing of isomalt oligosaccharide composition and can be used in food industry. Proposed method comprises interaction of starch suspension containing 15 % to 45 wt% of starch with 0.0001-10 % (mass fraction of solid substances) amylase fluidizing enzyme at temperature of approximately 95 °C to 125 °C and pH of approximately 5-7 for 60-210 minutes; interaction of obtained product with 0.000110 % (mass fraction) Beta-amylase maltogenic enzyme or fungal alpha-amylase maltogenic enzyme and 0.0001-10 % (mass fraction) invertase having trans glucoside activity, at temperature higher than 10 °C and below 85 °C and at pH higher than 2 and lower than 10, where maltogenic enzyme and invertase reacting simultaneously with said product for producing isomalt oligosaccharides.EFFECT: novel efficient method of producing valuable carbohydrates.13 cl, 4 ex, 1 tbl, 2 dwg

Tetravalent neoglycoconjugates with carbohydrate branching core and method for obtaining thereof // 2575925
FIELD: chemistry.SUBSTANCE: invention relates to obtaining tetravalent neoglycoconjugates with carbonhydrate branching core and derivative of L-glutamic acid in hydrophobic block, suitable for preparation of liposomes, of formula . Method includes conjugation of azidoethyl carbonhydrate derivative, selected from 1-O-(2-azidoethyl)-α-D-mannopyranozide or 1-O-(2-azidoethyl)lactoside, with bishexadecyl-N-({3-[N'-2,3,4,6-tetra-O-propynyl-β-D-glycopyranozyloxy]-carbamoyl}-propyonyl)-L-glutamate.EFFECT: increase of affinity of carbohydrate residuals to receptors, effective method of thereof obtaining.2 cl, 1 dwg, 8 ex

Glucooligasaccharides, containing (alpha 1-4) and (alpha-1-6) glycosidic linkages, their application and methods of their obtaining // 2571206
FIELD: biotechnologies.SUBSTANCE: method of obtaining of the mix of glucooligasaccharides containing two or more series (α1→6) glycosidic linkages and two or more series (α1→4) glycosidic linkages is offered. Poly-and/or oligosaccharide comprising on non-restorable end at least two (α-1→4)-linked units of D-glucose, are forced to contact with the enzyme α-glucanotransferase enzyme. The named enzyme is GTFB type of enzyme and splits (α1→4) and creates new (α1→4) and (α1→6) glycoside links. The released linear glucooligasaccharides and their mixes obtained by the named method, their use as fibre-prebiotic in food composition are offered. Also the genetically modified GTFA-type glucan sucrase enzyme including at least two mutations indicated in the invention formula and with GTFB-type activity is also offered. The named modified enzyme splits (α1→4) and creates new (α1→4) and (α1→6) glycosidic linkages, using as a substratum the poly-and/or the oligosaccharides containing (α1→4)-linked units of D-glucose on its non-restoring end.EFFECT: inventions allow to obtain partially indigestible derivatives of starch.19 cl, 2 tbl, 8 dwg

ixture for low-molecular sugar fermentation // 2563516
FIELD: food industry.SUBSTANCE: invention relates to a mixture for low-molecular sugar fermentation. The proposed mixture contains low-molecular sugar, preliminarily milled material of lignocellulosic biomass, having porosity value equal to at least 35%, and a solvent represented by water. The low-molecular sugar is represented by acarbohydrate or its derivative with molecular weight (from the formula) equal to less than 2000. The lignocellulosic biomass is subjected to radiation with an electron beam; as a result, biomass containing a carboxylic group is generated. The radiation dose is equal to at least 5 millirad.EFFECT: invention ensures effective processing of biomass for manufacture of such beneficial products as fuel.12 cl, 56 dwg, 78 tbl, 32 ex
Oligosaccharide mixture and food product containing said mixture, particularly infant formula // 2560190
FIELD: chemistry.SUBSTANCE: present invention relates to an oligosaccharide mixture and a food product containing said mixture. The oligosaccharide mixture contains 5-70 wt % of at least one N-acetylated oligosaccharide, 5-90 wt % of at least one galacto-oligosaccharide, 2-50 wt % of at least one sialylated oligosaccharide and 2-70 wt % of at least one fucosylated oligosaccharide. The fucosylated oligosaccharide is selected from a group which includes 2′-fucosyllactose, 3-fucosyllactose, difucosyllactose, lacto-N-fucopentaoses, lacto-N-difucohexaose I, monofucosyllacto-N-hexaose, difucosyllacto-N-hexaose I and difucosyllacto-N-neohexaose II. A food product containing said mixture is disclosed.EFFECT: use of said mixture enables to prevent or reduce the occurrence of food allergies and food allergy related health effects, and also raises oral tolerance to allergens.25 cl, 2 ex, 1 tbl

External cow lipopolysaccharide epitope h. pylori // 2558257
FIELD: chemistry.SUBSTANCE: present invention relates to biotechnology and provides a α1,6-glucan-containing compound of Helicobacter pylori. The present invention also discloses a conjugate for inducing immune response against H.pylori, which contains said compound conjugated with a carrier protein. The present invention also discloses an immunogenic composition, use of said composition and a method of inducing immune response against H.pylori using said composition. The present invention also discloses immune serum for neutralising H.pylori in mammals, which is obtained by immunising said mammal with an immunogenic composition containing said immunogenic composition. The present invention discloses an antibody which recognises said α1,6-glucan-containing compound of H.pylori, use of said antibody and a method of inducing complement-mediated bacteriolysis of H.pylori strains which express α1,6-glucan using said antibody.EFFECT: invention improves the effectiveness of immunogenic compositions against Hpylori.27 cl, 8 dwg, 21 tbl, 11 ex

Colon cleansing l-sugar agent and using it // 2536691
FIELD: medicine, pharmaceutics.SUBSTANCE: present invention refers to L-glucose monohydrate used for colon cleansing, to a method for preparing it, to compositions containing above monohydrate, to a method of colon cleansing and to a kit for colon cleansing. Generally, presented L-glucose monohydrate has an X-ray powder diffraction (XRPD) pattern containing one or more characteristic peaks at 9.24, 18.46, 19.78, 20.24 and 28.36±0.2 degrees 2θ, optical rotation from -52.5 to -53.3 degrees calculated with reference to anhydrous L-glucose, and purity of at least 99%. The method for preparing L-glucose monohydrate involves (a) adding L-glucose to water and heating at low pressure, and (b) cooling the prepared solution to form L-glucose monohydrate.EFFECT: preparing the colon cleansing composition.22 cl, 1 dwg, 2 tbl, 33 ex

ethods and compositions described as synthetic beta-1,6-glucosaminoligosaccharides // 2532911
FIELD: medicine, pharmaceutics.SUBSTANCE: invention refers to oligosaccharide carrier conjugates effective for treating and preventing infectious diseases and containing an oligosaccharide conjugated with the carrier through a linker of formulas VIIIa or VIIIb: wherein n is more than 1, m is specified in 1-10, p is specified in 1-20 and R represents H or alkyl; the linker is bound to an oligosaccharide oxygen atom through a terminal CH2 group, and the linker is bound through a terminal CO group with an amino group of the carrier compound by an amide bond; the oligosaccharide is β-1-6 related glucosamine, and the carrier is peptide, protein, polysaccharide, nucleic acid, lipid or tetanus toxoid.EFFECT: there are presented new conjugates effective to stimulate the immune response, and a method for preparing them.21 cl, 12 ex, 23 dwg, 1 tbl

Regenerative cleaning of preprocessed biomass flow // 2508929
FIELD: process engineering.SUBSTANCE: invention relates to removal of furfural and acetic acid from the flow containing water, C5, C6 and compound selected from acetic acid and furfural. Proposed method comprises interaction of the first flow with adsorptive medium which interacted preliminary with the second flow containing the same components. Adsorptive medium was regenerated, with no contact with the second flow, so that at least 70% of adsorbed furfural and acetic acid were desorbed and at least 60% of C5, C6 were adsorbed in the medium. Said medium represents spherical particles of activated charcoal. C5 is the sum of arabinan and xylene while C6 is glucan.EFFECT: higher efficiency of byproduct removal.11 cl, 3 tbl
Oligosaccharide ingredient // 2497827
FIELD: chemistry.SUBSTANCE: oligosaccharide ingredient, which is intended to increase content of sialic acid in baby formula, contains glycosylated amino acids and peptides of general formula RnSacm, where R is an amino acid residue, Sac is a monosaccharide selected from a group comprising N-acetyl-neuraminic acid, N-acetyl galactosamine and galactose n has a value from 1 to 10, under the condition that if n=1, then R is a threonine residue or a serine residue and if n has a value between (2) and (10) the peptide contains at least one threonine or serine residue, m has a value from 2 to 4 and at least 20 mol % of the ingredient is N-acetyl-neuraminic acid, where the oligosaccharide component contains 10-25 mol % N-acetyl galactosamine, 10-25 mol % galactose, 20-50 mol % N-acetyl-neuraminic acid and 15-50 mol % threonine or serine or mixture thereof.EFFECT: obtaining an oligosaccharide ingredient intended for increasing content of sialic acid in baby formula.6 cl, 2 ex
ethod of catalytic conversion of cellulose in hexitols // 2497800
FIELD: chemistry.SUBSTANCE: invention relates to field of processing renewable raw material (in particular, cellulose) into raw material for chemical synthesis and biofuels. Method of catalytic conversion of cellulose in hexitols includes carrying out process of hydrolytic hydration of cellulose for 3-7 minutes at temperature 240-250°C under partial pressure of hydrogen 55-65 atm and with mixing of reaction medium in presence of ruthenium catalyst, according to invention as substrate for ruthenium catalyst applied is hypercrosslinked polystyrene of MN 270 grade, and content of ruthenium in catalyst constitutes from 1.0 to 1.5 wt % of catalyst weight. Mixing of reaction mixture is realised by propeller mixer, numbers of turns of which constitutes 580-620 rev/min.EFFECT: increased method efficiency.2 cl, 16 ex
Oligosaccharide ingredient // 2496782
FIELD: chemistry.SUBSTANCE: invention relates to an oligosaccharide ingredient for adding to nutritional compositions or food products, which contains glycosylated amino acids and peptides of general formula RnSacm, where R is an amino acid residue, Sac is a monosaccharide selected from a group comprising N-acetyl-neuraminic acid, N-acetyl-galactosamine and galactose, n has a value between 1 and 10 with the proviso that if n equals 1, R is a threonine residue or a serine residue and if n has a value between 2 and 10, the peptide contains at least one threonine or serine residue, m has a value between 2 and 4 and at least 20 mol % of the ingredient is N-acetyl-neuraminic acid, where the oligosaccharide ingredient contains 15-20 mol % N-acetyl-galactosamine, 15-25 mol % galactose, 20-50 mol % N-acetyl-neuraminic acid and 15-25 mol % threonine or serine or mixture thereof.EFFECT: obtaining an oligosaccharide ingredient.7 cl, 1 tbl, 2 ex

ethod of fermenting low-molecular weight sugar to ethanol // 2490326
FIELD: chemistry.SUBSTANCE: method of fermenting low-molecular weight sugar to ethanol involves mixing low-molecular weight sugar, one or more fermenting microorganisms and modified biomass, fermenting the low-molecular weight sugar in conditions that are suitable for converting sugar to ethanol. The modified biomass has bulk density of less than about 0.5 g/cm3 and contains cellulose fibres that have been substantially irradiated and contain carboxylic acid groups. The fermenting microorganism includes yeast selected from a group consisting of S. cerevisiae and P. Stipitis or Zymomonas mobilis bacteria.EFFECT: invention enables to obtain ethanol with output of at least 140%.17 cl, 40 dwg, 78 tbl, 32 ex

New sulphated oligosaccharide derivatives // 2483074
FIELD: medicine, pharmaceutics.SUBSTANCE: present invention refers to new compounds of general formula I [X]n-Y-ZR1R2, wherein the radicals are specified in the description, effective as heparan sulphate-binding protein inhibitors. The invention also refers to a pharmaceutical or veterinary composition having heparan sulphate-binding protein inhibitory activity for preventing or treating a disorder in a mammal, and to the use of these compounds and compositions for antiangiogenic, antimetastatic, anti-inflammatory, antimicrobial, anticoagulant and/or antithrombotic therapy in a mammal.EFFECT: preparing the new compounds of general formula I [X]n-Y-ZR1R2, wherein the radicals are specified in the description, effective as the heparan sulphate binding protein inhibitors.10 cl, 31 ex, 11 tbl, 40 dwg

Galactomannan containing compositions, and method for preparing them // 2481351
FIELD: medicine, pharmaceutics.SUBSTANCE: present invention refers to a novel compound of structural formula I representing low molecular galactomannan having molecular weight about 702 Daltons and molecular formula C24H46O23. The compound is prepared of parts of the plant Trigonella foenum graecum selected from the group consisting of roots, shoots, leaves, seeds; preferentially seeds. What is also declared is a composition comprising an effective amount of a compound, optionally together with the adjuvants, for improving the body composition, increasing strength and positive effect on the factors associated with pre-diabetic and diabetic conditions.EFFECT: invention refers to a method for preparing the compound of formula I, as well as to a method of treating pre-diabetic and diabetic diseased conditions in an individual in need thereof.23 cl, 7 ex, 2 tbl, 8 dwg

Inclusion complexes // 2480987
FIELD: chemistry.SUBSTANCE: invention can be used in agricultural chemistry. A mixture of inclusion complexes of two cyclic compounds is obtained, where for the first of said cyclic compounds A is a double bond and R is a saturated C1-C4 alkyl and for the second of said cyclic compounds, A is a single bond and R is an unsaturated C1-C4 alky, wherein in said unsaturated C1-C4 alkyl, the double bond is adjacent to one carbon ring atom, into molecules of a macrocyclic compound where x=6, 7 or 8, or modified derivative thereof, through the following steps: 1) simultaneous production of said cyclic compounds by reacting a halogenated carbene with a base in the presence of cucurbituril or calixarene; 2) cleaning, if needed, the cyclic compounds obtained at step 1 from undesirable impurities; 3) reaction of the obtained cyclic compounds with said macrocyclic compound to form a mixture of inclusion complexes of two cyclic compounds into molecules of a macrocyclic compound. Steps 1) and 3) can be combined. The mixtures are used on plants and fruits.EFFECT: invention increases yield.2 cl, 4 tbl, 1 ex

icronisation of polyols // 2479587
FIELD: chemistry.SUBSTANCE: described is a method for micronisation of polyols, comprising the following steps: a) providing a solid polyol, having chemical formula CnH2n+2On, which is solid at 20-25°C, b) feeding the polyol into a jet mill and applying pressure using nitrogen, and c) collecting the micronised polyol. Micronised polyols obtained using the disclosed method, having particle size distribution (d50) from 20 to 60 mcm and a flowability below or equal to 5 s/100 g, preferably below 5 s/100 g, are disclosed. The micronised polyols, although having lower particle size distribution compared to the corresponding milled polyols, have improved flowability. Most preferably, the polyol is selected from a group comprising one or more of such compounds as maltitol, isomalt, mannitol, sorbitol, xylitol and erythritol.EFFECT: preferred polyols are characterised by a compressibility index equal to or higher than 40.14 cl, 4 ex

Dextrane functionalised by hydrophobic amino acids // 2476437
FIELD: medicine, pharmaceutics.SUBSTANCE: present invention refers to new biologically compatible polymers of dextran, namely functionalised dextran of general formula I. Dextran is functionalised by at least one residue of hydrophobic alpha-amino acid with said alpha-amino acid is cross-linked or coupled with dextran by a coupling branch R and one function F which have the values specified in the description. A residue of hydrophobic alpha-amino acid means an interaction product of amine of amino acid and acid of the coupling branch with dextran being amphiphilic at neutral pH, while a polymerisation degree of dextran makes 10 to 10000. According to one version of the implementation, hydrophobic amino acid is specified in phenylalanine, leucine, isoleucine and valine and their alcohol, amide or decarboxylated derivatives.EFFECT: invention also refers to a pharmaceutical composition containing one of dextrans under the invention.12 cl, 6 ex

ethod of extracting fructose from aqueous solutions using mixture of ethyl acetate and acetone // 2471806
FIELD: chemistry.SUBSTANCE: invention relates to analytical chemistry and biotechnology and can be used to extract carbohydrates from aqueous solutions for subsequent quantitative determination thereof. The engineering problem of the invention is designing a method of extracting fructose from aqueous solutions using a mixture of ethyl acetate and acetone, which increases extraction of fructose from aqueous solutions without using harmful organic solvents. To solve the engineering problem, the invention provides a method of extracting fructose from aqueous solutions using a mixture of ethyl acetate and acetone, characterised by that a weighed portion of fructose is put into a 100 cm3 measuring flask and, while stirring, filling up to the maximum level with a saturated solution of a salting-out agent in form of ammonium sulphate; a mixture ethyl acetate and acetone, taken in ratio of 4:1 is added to the obtained water-salt solution of fructose, and with volume ratio of the water-salt solution of fructose and the mixture of ethyl acetate and acetone of 15:1, fructose is extracted; after demixing, the aqueous phase system is separated from the organic phase and analysed via ascending thin-layer chromatography, the mobile phase used being the mixture: propyl alcohol - ethyl acetate - water - acetic acid in ratio of 5:5:3:1; the calibration curve of spot area versus fructose concentration is used to find fructose concentration in the aqueous solution; the aqueous phase is once more mixed with ammonium sulphate and fructose is re-extracted using the method given above; the aqueous phase is separated and fructose content therein is determined by ascending thin-layer chromatography; knowing fructose concentration in the aqueous and organic phase, distribution coefficient (DI), degree of extraction of fructose after the first extraction (RI, %) and degree of extraction of fructose after twofold extraction (Rtotal, %) are calculated using the formulae: where c0 and cv denote fructose concentration in the organic and aqueous phases; f is the ratio of volumes of equilibrium aqueous and organic phases, where RII is the degree of extraction of fructose after the second extraction, %.EFFECT: disclosed method of extracting fructose provides virtually complete extraction of fructose from aqueous solutions without using harmful organic solvents and enables to determine fructose in the concentrate.1 cl, 1 ex

ethod of obtaining catalyst on porous metal oxide carrier for carbohydrate oxydation // 2468861
FIELD: chemistry.SUBSTANCE: invention relates to method of obtaining and application of obtained gold catalyst for oxidation of aldehyde groups into carboxyl functional groups. By means of catalyst it is possible to oxidise glucose into gluconic acid, and lactose - into lactobionic acid. Method of obtaining catalyst on porous metal oxide carrier for carbohydrate oxidation is characterised by the fact that gold precursor is precursor, obtained by ionic float extraction from gold scrap, and precursor reducer is sodium hydrazine hydrate or borohydrate.EFFECT: use of wastes ensures material-preserving factor of the method.1 cl, 5 ex

Divalent l-glutamic acid diether-based neoglycoconjugates and production method thereof // 2462471
FIELD: chemistry.SUBSTANCE: use of two carbohydrate residues in the structure of a divalent neoglycoconjugate enables sixfold increase in binding efficiency compared to the monovalent derivative of monolactosyl-L-glutamate-succinate-dihexadecyl-L-glutamic acid, which can be used to produce high-efficiency directed systems for delivering medicinal agents. EFFECT: high affinity of carbohydrate residues to proteins by forming new divalent ligands based on L-glutamic acid diether, which are specific to corresponding receptors which contain residues of different carbohydrates, via a 1,3-dipolar cycloaddition reaction.2 cl, 2 ex

ethod of producing tagatose using soya oligosaccharide // 2451688
FIELD: chemistry.SUBSTANCE: invention relates to a method of producing tagatose. The method involves the following steps: a) extraction of soya oligosaccharide from by-products of soyabean whey; b) hydrolysis of soya oligosaccharide using α-galactosidase which does not contain invertase; c) isomerisation of the hydrolysed sugar solution using arabinose isomerase to obtain an isomerised product containing mixed sugar containing saccharose, galactose and tagatose as the basic components; and d) separation of the isomerised solution via chromatography.EFFECT: use of the present method simplifies chromatographic separation of a sugar solution containing at least saccharose, galactose and tagatose.15 cl, 10 tbl, 11 ex, 18 dwg
ixture of oligasaccharides // 2442438
FIELD: food industry.SUBSTANCE: method of oligasaccharide mixture production from animal milk which has the same range of oligasaccharides as milk it was produced from includes the following stages: (a) concentration of deproteines milk material to the total content of dry substance from 50 to 75% given the condition that temperature shall not rise to the level at which hydrolisis or sialic acid reduction take place; (b) concentrated milk is subject to partial lactose removal and preparation of solution with lactose: oligasaccharide ratio less than 250, and (c) demineralization of milk material - at this the stage of demineralization is conducted either before concentration stage or after lactose removal. Also invention refers to the mixture of oligasaccharides recieved this way from animal milk and food products containing the mentioned mixture of oligasaccharides.EFFECT: receipt of mixture of oligasaccharides.18 cl, 2 tbl, 3 ex

ethod of regulating antigen immunogenicity // 2440141
FIELD: medicine.SUBSTANCE: method of antigen immunogenicity regulation includes incorporation of antigen into structure of immunostimulating complex (TI-complex) - antigen carrier. As protein antigen used is porin from Yersinia pseudotuberculosis, its carrier being immunostimulating complex in form of ultramicroscopic tubules (TI-complex), which consists of mixture of triterpene glycoside cucumarioside A2-2 (CD), cholesterol and polar lipid of monogalactosyldiacylglyceride (MGDG) from marine macrophytes with weight ratio CD:cholesterol:MGMD 6:2:4. Variation of lipid antigen surrounding is achieved due to application of MGDG with various fatty acid composition, separated from various species of marine macrophytes.EFFECT: method makes it possible to carry out targeted regulation of immune response to antigen not only due to change of quantitative but also qualitative composition of the complex, and obtain new optimal means of specific prevention against causative agents of pseudotuberculosis and other infectious diseases.3 cl, 6 dwg, 2 tbl, 6 ex
ethod of producing d-glucose labelled with carbon isotope in position 1 // 2425051
FIELD: chemistry.SUBSTANCE: invention relates to a method of producing D-glucose which is labelled with carbon isotopes 13C or 14C in position 1, involving condensation of D-arabinose with labelled nitromethane, taken in equimolar ratio, in dimethylsulphoxide in the presence of a base, followed by benzene extraction of excess dimethylsulphoxide, freeze-drying from benzene, cooling the reaction mixture of 1-desoxy-1-nitroalditol salts to liquid nitrogen temperature, adding water, followed by slow thawing while stirring and decomposition of 1-desoxy-1-nitroalditol salts contained in the obtained aqueous solution, and extraction of D-glucose via chromatography. Said method increases output and improves the process.EFFECT: obtaining products used in medicine as isotopic diagnostic agents, as well as in scientific research and other fields.3 cl, 4 ex

Arylsulphonamide-substituted hydroxamic acid derivatives as matrix metalloproteinase inhibitors // 2406721
FIELD: chemistry.SUBSTANCE: present invention relates to novel arylsulphonamide-substituted hydroxamic acid derivatives of formula (I) , where R1 is a C1-C7alkyl group substituted with at least two OH groups or one X-monosaccharide, where X is an O atom, R2 is selected from a group consisting of H, C1-C7alkyl substituted with one hydroxyl group, R3 is selected from a group consisting of oxy- C1-C7alkyl, phenyl, and to pharmaceutically acceptable salts thereof, having good solubility and selective inhibiting effect on matrix metalloproteinase MMP-12, MMP-8, MMP-13, MMP-1, to pharmaceutical compositions and cosmetic agents based on said compounds, as well as to methods of inhibiting MMP-12, MMP-8, MMP-13, MMP-1.EFFECT: obtaining novel arylsulphonamide-substituted hydroxamic acid derivatives.13 cl, 4 tbl, 8 ex

Double antithrombotic inhibitors containing biotin residue // 2403259
FIELD: medicine, pharmaceutics.SUBSTANCE: claimed invention relates to antithrombotic compound of formula (I) (oligisaccaride-spacer-A(I)), where oligosaccharide is negatively charged pentasaccharide residue of formula given lower, where R5 - OCH3 or OSO3 -, charge is compensated by positively charged counterions, and where pentasaccharide residue is obtained from pentasaccharide which has AT-III mediated anti-Xa activity per se; spacer is, in fact, pharmacologically inactive movably bound residue, which has from 10 to 50 atoms long chain; A is residue of -CH[NH-SO2-R1] [CO-NR2-CH(4-benzamidin)-CO-NR3R4], where R1 - 4-methoxy-2,3,6-trimethyphenyl; R2 - H; NR3R4 is piperidinyl group or its pharmaceutically acceptable salt or derivative, where amino group of amidin residue is protected by hydroxyl or (1-6C) alcoxycarbonyl group; where spacer of formula I compound contains one covalent bond with biotin residue of formula -(CH2)4-NR-BT, where R - H or (1-4C)alkyl and BT is residue . Invention also relates to pharmaceutical composition based on formula I compounds for treatment or prevention of thrombosis or other connected with thrombin diseases.EFFECT: elaboration of antithrombotic compound for treatment or prevention of thrombosis or other, connected with thrombin diseases.6 cl, 3 tbl, 2 dwg, 3 ex

Sulphated derivatives of oligosaccharides // 2392281
FIELD: chemistry.SUBSTANCE: invention refers to sulphated oligosaccharides of the general formula X-[Y]n-Z-UR1, where X, Y and Z each are the same hexose monosaccharide fragment selected out of group including glucose, mannose, altrose, allose, talose, galactose, idose and gulose, adjoining monosaccharide fragments are bound in 1→2, 1→3, 1→4, and/or 1→6 pattern by glycoside bonds, and each carbon atom not binding X, Y and Z groups is bound by single bond with UR group, with exception for carbon atom in 1 position of Z monosaccharide, to which UR1 group is bound by single bond; where n is an integer within 0 to 6; U is O atom or NH; each R is independently C2-C6-alkenyl, benzyl, SO3M or H, where M is any pharmaceutically acceptable cation of alkali metal or organic amine, or R form N3 together with U; R1 is C1-C12alkyl, benzyl, PEG monomethyl ether or its derivative, C1-C12alkylazide, , or , in the form of ester, free acid, free base or hydrate. Also invention refers to pharmaceutical or veterinary composition based on claimed compounds, for disorder prevention or treatment for mammals in case of proliferate retinopathy, solid tumour and/or metastasis result, coagulation/thrombosis and/or virus infection of organism. Additionally invention refers to application of claimed compounds in medicine production for disorder prevention or treatment for mammals in case of proliferate retinopathy, solid tumour and/or metastasis result, coagulation/thrombosis and/or virus infection of organism.EFFECT: increased efficiency of compound application in medicine.11 cl, 3 tbl, 1 dwg, 16 ex

odified saccharides with improved stability in water // 2338753
FIELD: chemistry.SUBSTANCE: invention pertains to modified polysaccharide in particular to modified polysaccharide Neisseria meningitidis of serogroup A, which preserves immunogenicity, but has improved stability. The modified polysaccharide is obtained from reaction of capsular polysaccharide, or its fragment - oligosaccharide, with CDI type bifunctional reagent, accompanied by reaction with an amino-compound, such as dimethylamine. Description is also given of modified polysaccharide conjugates and vaccines, which are obtained from such conjugates.EFFECT: obtaining modified saccharide.70 cl, 17 dwg

Oligosaccharides modified with fluorophorous marker for determination of glycanase activities and fermentative method for obtaining same // 2333915
FIELD: chemistry.SUBSTANCE: claimed invention relates to new oligosaccharides modified with fluorophorous marker, namely, 4-O-methylumbelliferyl β-D-xylopiranosil-(1,4)-bis-[O-β-D-xylopiranosil-(1,4)]-β-D-xylopiranoside, 4-O methylumbelliferyl β-D-xylopiranosil-(1,4)-tris-[O-β-D-xylopiranosil-(1,4)]-β-D-xylopiranoside, 4-O-methylumbelliferyl β-D-xylopiranosil-(1,4)-tetrakis-[O-β-D-xylopiranosil-(1,4)]-β-D-xylopiranoside. It is intended for measuring activities of both pure and coarse preparations of β-xylanases. The invention also relates to fermentative synthesis of oligosaccharides with formula: , which lies in incubation of β-xylosidase from Aspergillus sp. at 37°C in neutral reaction medium in presence of aryl-β-D-xylopiranoside and 4-O-methylumbelliferyl-β-D-xylopiranoside.EFFECT: possibility to measure activities of both pure and coarse preparations of β-xylanases.4 cl, 1 dwg, 2 tbl

Carrier of antigens // 2322259
FIELD: biotechnology, immunology.SUBSTANCE: invention proposes a carrier of antigens as a lipid complex consisting of glycoside, cholesterol and lipid. As glycoside a carrier comprises holotoxin A1, and as lipid - monogalactosyldiacyl glycerides (MGDG) of sea macrophytes taken in the weight ratio 3 (holotoxin A1) : 2 (cholesterol) : (2-6) (MGDG). Prepared glycoside-cholesterol-lipid carrier shows stretched threadlike-tubular structure. Use of the prepared carrier of antigens allows enhancing the immunogenic activity of vaccine preparations, and to decrease or remove completely hemolytic toxicity of holotoxin A1, and to eliminate inflammatory, painful, toxic and hemolytic effects of vaccines.EFFECT: improved and valuable medicinal properties of carrier and vaccines.4 dwg, 11 ex

ethod for preparing sulfated derivatives of arabinogalactan possessing anticoagulating and hypolipidemic activity // 2319707
FIELD: chemical-pharmaceutical industry.SUBSTANCE: invention relates to a method for synthesis of arabinogalactan-base sulfated biopolymers wherein arabinogalactan is the main polysaccharide from Siberian larch. Invention describes a method for preparing sulfated derivatives of arabinogalactan that involves interaction of arabinogalactant in dimethylsulfoxide medium in the ratio 1:3 with a sulfating agent representing complex SO3 - dimethylformamide in the concentration of SO3 ≥18%. The process is carried out at continuous stirring, at temperature 20°C for 30 min followed by isolation of product both in acid and saline forms. The substitution degree of biopolymer macromolecule is 8.1-12.65% as measured for the quantitative content of sulfur. Synthesized sulfated derivatives of arabinogalactan retain the structural organization, water-solubility and membranotropicity of natural polysaccharide, and elicit high physiological (anticoagulating and hypolipidemic) activity also.EFFECT: improved method of synthesis.2 cl, 3 dwg, 3 ex

ethod for preparing carrier of antigens based on lipids from sea macrophytes and triterpene glycoside cucumarioside // 2319506
FIELD: biotechnology.SUBSTANCE: invention relates to carriers of antigens. Proposed carrier represents lipid-saponin complex consisting of a mixture of triterpene glycoside, cholesterol and glyceroglycolipid. Cucumarioside A2-2 is used as triterpene glycosides, and monogalacosyldiacylglycerides of sea macrophites are used as glyceroglycolipids. Method involves mixing solutions of cholesterol and glyceroglycolipid in chloroform, evaporation of mixture until dry under vacuum, addition of 3 weight parts of 0.4% aqueous solution of cucumarioside. Then the mixture is solubilized and the total concentration of cholesterol and glyceroglycolipid is brought about to 2 mg in 1 ml of suspension with phosphate-saline buffer at pH 7.2 followed by sonication of the prepared suspension by ultrasonic oscillation device at frequency 20 kHz for 5 min. Method provides preparing the effective adjuvant form of the carrier.EFFECT: improved preparing method of carrier.6 cl, 8 dwg, 13 ex

Associate containing trehalose and calcium chloride as crystalline monohydrate or anhydrous crystal // 2317299
FIELD: cosmetics, pharmacy.SUBSTANCE: invention relates to associates containing trehalose and calcium chloride as a crystalline monohydrate comprising above said components and water in the molar ratio = 1:1:1, or as anhydrous crystal containing above said components in the molar ratio = 1:2. Indicated crystals of two species of associates of trehalose and calcium chloride can be used in different branches in production of foodstuffs, cosmetic agents and others.EFFECT: valuable properties of compounds.3 cl, 20 tbl, 15 dwg, 30 ex

Enzymatic method for preparing 4-o-beta-d-galactopyranosyl-d-xylose // 2316593
FIELD: biotechnology, biochemistry, enzymes.SUBSTANCE: invention relates to a method for preparing 4-O-β-D-galactopyranosyl-D-xylose used for in vivo assay of lactase activity in human intestine. Method involves interaction of D-xylose with β-D-galactopyranoside for 2-48 h in a solution at pH 5-9, at temperature that changes from the mixture freezing point to 45°C, and the following addition of 10-1000 U of β-D-galactosidase per 1 g of β-D-galactopyranoside. Reaction is terminated by inactivation of enzyme followed by isolation and crystallization of fraction containing 4-O-β-D-galactopyranosyl-D-xylose in the crystallization mixture chosen from acetone/methanol = (5-1):(20-1) and acetone/water = (5-1):(20-1). Invention provides increasing content of 4-O-β-D-galactopyranosyl-D-xylose in the final mixture.EFFECT: improved preparing method.42 cl, 2 tbl, 9 ex

Pentasaccharide conjugate, method for its preparing and pharmaceutical composition based on thereof // 2266913
FIELD: organic chemistry, chemical technology, medicine, pharmacy.SUBSTANCE: invention describes a novel pentasaccharide conjugate of the formula (I): wherein R represents independently -SO- 3 or -CH3; insert represents a flexible insert of length 13-25 atoms. Charge of a pentasaccharide residue is equilibrated with positively charged counterions and the total amount of sulfate groups in a pentasaccharide residue is 4, 5 or 6. Also, invention relates to a method of its preparing and pharmaceutical composition based on thereof and used in treatment of diseases mediated or associated with thrombin.EFFECT: improved preparing method, valuable medicinal properties of conjugate.9 cl, 2 ex

Carbohydrate derivatives // 2210573
The invention relates to a carbohydrate derivative of the formula (I), where R1represents (1-4C)alkoxy; R2, R3and R4independently represent (1-4C)alkoxy or OSO3-; the total number of sulfate groups is 4-6; and their pharmaceutically acceptable salts

The method of receiving lactulose and installation for its implementation // 2203959
The invention relates to food industry, namely breast

Derivative benzopyran-2-it, the way they are received, containing pharmaceutical composition and the intermediate // 2194052
The invention relates to a derivative of benzopyran-2-it formula I, where R1denotes a hydrogen atom, a hydroxyl radical, optionally substituted alkyl radical, optionally interrupted by oxygen atom, sulfur or nitrogen, CNS radical or a radical NRcRdX denotes an oxygen atom or a radical N-N(CH3)2or radical NOalc2where alc2denotes optionally substituted alkyl radical, optionally interrupted by oxygen atom, sulfur or nitrogen,2denotes a hydrogen atom or halogen atom, R3denotes a hydrogen atom, an alkyl radical or a halogen atom, R4denotes a radical NRgRh, optionally substituted aryl or heteroaryl radical, R5denotes a hydrogen atom or O-alkyl, R6denotes alkyl or CH2-O-alkyl, R7denotes a hydrogen atom or alkyl
 
2550858.
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