Indexing scheme associated with subclasses c12c-c12q and or c12s, relating to micro-organisms (C12R)

C   Chemistry; metallurgy(318327)
C12R              Indexing scheme associated with subclasses c12c-c12q; or c12s, relating to micro-organisms(2819)
Recombinant producer of human omega-amidase nit2 based on escherichia coli // 2642323
FIELD: biotechnology.SUBSTANCE: invention relates to a recombinant strain of Escherichia coli BL21 (DE3; pQE-Nit2). This strain was obtained by transforming the Escherichia coli strain BL21 (DE3) by the plasmid construct pQE-Nit2, which is under the control of the T5 phage promoter and is designed to produce human ω-amidase (Nit2) when cultivated on nutrient media based on peptone, yeast extract and glucose. The peculiarity of the Nit2 gene in the pQE-Nit2 construct is optimized codon composition, as well as presence of an artificially introduced 6His- affinity tag immediately after the initiator codon ATG (Met), which allows one-step product purification by metal-affinity chromatography on Ni-NTA-agarose.EFFECT: invention allows to obtain human ω-amidase with an elevated yield.2 dwg, 1 tbl, 6 ex
Bacterial strains kit used for training on microbiology and plague laboratory diagnostics methods // 2642322
FIELD: biotechnology.SUBSTANCE: invention is a kit containing avirulent Yersinia pestis strains of pestis subspecies, orientalis biovar (KM 2011), pestis subspecies, antiqua biovar (KM 2008, KM 2012, KM 260 (12), km 130 (3)), pestis subspecies, medievalis biovar (KM 2010, 2014, 2024), caucasica subspecies (KM 2013), Yersinia pseudotuberculosis strains (KM 2004, 2005, 2006), Yersinia enterocolitica (KM 2007) and Pasteurella multocida strain (KM 2003), deposited in the State Collection of Pathogenic Bacteria "Microbe". A kit of strains can be used to train on microbiology and methods for laboratory diagnosis of plague.EFFECT: invention reduces the risk of students' infection.9 ex

Probiotic strains for treatment and/or prevention of diarrhea // 2642320
FIELD: biotechnology.SUBSTANCE: method for selection of a probiotic Bacillus strain, which includes assessment of the impact of the test strain on CFTR protein expression or NHE3 protein expression; Bacillus subtilis probiotic strain reducing CFTR protein expression and/or increasing NHE3 protein expression, deposited in the CNCM under accession number I-2745; cells obtained by cultivation of probiotic Bacillus strain, and a composition containing the effective number of cells for use in treatment and/or prevention of diarrhea.EFFECT: regulation of water absorption in the colon in the treatment and/or prevention of diarrhea.21 cl, 6 dwg, 2 ex
ethod for production of vaccine against brucellosis of small cattle // 2642316
FIELD: biotechnology.SUBSTANCE: method for production of vaccine against the brucellosis of small cattle provides seeding and growing strain of Brucella melitensis Rev-1 on a nutritional medium, containing pancreatic digest of casein of a high decomposition degree, yeast extract, sodium chloride, glucose, glycerol, and agar-agar in the specified component ratio within 72 hours at 37°C. The grown vaccine culture is washed from the surface of the agar by a drying medium and brought to a concentration of 80-140 billion m.c. per 1 ml. It is checked for purity and dissociation, packed in ampoules and subjected to freeze-drying.EFFECT: increase in the yield of Brucella melitensis Rev-1 strain in the S-form.1 tbl, 1 ex

Lactobacillus strain having inhibitory activity against yeasts and mould fungi (versions), and its application // 2642306
FIELD: food industry.SUBSTANCE: strain of bacteria Lactobacillus paracasei DSM 25832, a strain of bacteria Lactobacillus plantarum DSM 25833, a strain of bacteria Lactobacillus plantarum DSM 25834, a strain of bacteria Lactobacillus plantarum DSM 25835, a strain of bacteria Lactobacillus plantarum DSM 25836, and a strain of bacteria Lactobacillus plantarum DSM 25837 are proposed. These strains have inhibitory activity against yeasts and mould fungi. The versions of a bacterial composition containing selected strains in combination with a bacterium of the Propionibacterium genus or another strain of the Lactobacillus genus are also proposed.EFFECT: obtaining a dairy product, applying in the yeast and mould fungi growth control method.17 cl, 8 dwg, 6 tbl, 3 ex

Polypeptide for blood sugar level reducing on basis of human glucagon-like peptide-1, recombinant producing strain e. coli and method of obtaining this polypeptide // 2642260
FIELD: biotechnology.SUBSTANCE: recombinant modified human glucagon-like peptide-1 (rmGLP-1), a method for its preparation, and strain E.coli RNCIM B-12555 are proposed. rmGLP-1 has the sequence of SEQ ID NO 1. rmGLP-1 is produced by culturing a recombinant strain E. coli RNCIM B-12555 to prepare the rmGLP-1 peptide precursor, then isolating the resulting precursor, its autocatalytic cleavage and purification of the target polypeptide. The mouse model shows that with subcutaneous or intramuscular administration, rmGLP-1 has indicators of sugar reduction activity and duration of action similar to that of the commercial preparation "Lysxemia" obtained using chemical synthesis and, for a minimum of 3 hours after administration, is able to effectively reduce the level of glucose in blood almost twice as compared with the control.EFFECT: abovementioned strain allows biosynthesis of the precursor rmGLP-1 at 40 percent of the total protein of the cells, which corresponds to the biosynthesis of rmGLP-1 at 13 percent of the total cell protein.3 cl, 3 dwg, 7 ex

Flavoured milk composition // 2641264
FIELD: food industry.SUBSTANCE: method to produce the flavoured milk composition, comprising the strain of Lactococcus lactis subspecies lactis biovar diacetylactis DL2126, VTT E-123249 as the diacetyl producing strain and diacetyl in the amount of at least 50 mg/kg in the milky medium, is carried out as follows. Provide the Lactococcus lactis strain subspecies lactis biovar diacetylactis DL2126, VTT E-123249 and the fermentation medium, containing lactose, citrate, yeast extract and/or casein hydrolysate, and mix the specified strain with the medium. Cultivate the mixture for 10 up to 28 hours under the conditions optimized for pH, aeration, mixing and temperature to provide the specified composition of flavoured milk. The flavoured milk composition application, while manufacturing of a milk product. The milk product contains the flavoured milk composition. The Lactococcus lactis subspecies lactis biovar diacetylactis DL2126, VTT E-123249 strain is used for the milk product manufacture. The Lactococcus lactis subspecies lactis biovar diacetylactis DL2126, VTT E-123249 strain is used, while the milk product manufacture. The milk product contains the Lactococcus lactis subspecies lactis biovar diacetylactis DL2126, VTT E-123249 strain. The butter manufacturing method from the raw milk is carried out as follows. Separate the raw milk into the cream, containing about 40% of fat, and the skim milk. Pasteurize the cream, beat them to separate the butter beans and buttermilk. Process the butter grains, add the flavoured milk composition to the grains, normalize the mixture and prepare the butter.EFFECT: manufacture of products with increased ability to produce diacetyl.24 cl, 5 dwg, 1 tbl, 6 ex
Probiotic strain lactobacillus gasseri and its composition with lactoferrin for prevention of diarrhea, nerrocessing enterherolite and sepisis caused by escherichia coli strains in prematurely born children // 2641258
FIELD: biotechnology.SUBSTANCE: Lactobacillus gasseri strain of BK-2918D VKM having antagonistic activity against Escherichia coli strains and a composition containing the strain of Lactobacillus gasseri ECM B-2918D and lactoferrin have been claimed. Probiotic strain lactobacillus gasseri and its composition with lactoferrin for prevention of diarrhea, nerrocessing enterherolite and sepsis caused by escherichia coli strains in prematurely born children.EFFECT: expansion of the assortment of probiotic drugs and nutrient mixtures that provide the formation of normal microflora of the gastrointestinal tract in prematurely born children.2 cl, 3 dwg, 3 tbl, 7 ex
ethod of purifying wastewater waters of oil-processing and petrochemical production from toluene // 2640260
FIELD: chemistry.SUBSTANCE: method of purifying toluene-containing wastewater from oil refineries and petrochemical enterprises is provided. The destruction of toluene is carried out with a concentration of not more than 50 mg/dm3 using a microorganism strain of Pseudomonas japonica PMBC in-11715 in conditions of immobilization of bacterial cells or in the absence of immobilization. The content of toluene decreases from 50 to 5 mg/dm3. Full purification from toluene is carried out within 48 hours.EFFECT: invention allows to increase the efficiency of the destruction of toluene.2 dwg, 3 tbl, 2 ex

Propionibacterium strain having inhibitory activity against yeasts and mould fungi (versions), and its application // 2640255
FIELD: food industry.SUBSTANCE: Propionibacterium acidipropionici strain DSM 25845, Propionibacterium freudenreichii strain of the subspecies shermanii DSM 25846, Propionibacterium freudenreichii strain DSM 25847, Propionibacterium thoenii strain DSM 25848, and Propionibacterium thoenii strain DSM 25849 are proposed. These strains have inhibitory activity against yeasts and mould fungi. The versions of a bacterial composition having inhibitory activity against yeasts and mould fungi and containing a selected strain in combination with a bacterium of the Lactobacillus genus or another strain of the Propionibacterium genus. A protective culture and a dairy product containing said strains are also proposed.EFFECT: obtaining a dairy product, applying separately or as part of a composition for controlling the growth of yeasts and mould fungi in the method of storing a dairy product.28 cl, 10 dwg, 3 tbl, 2 ex
ethod for production of nutrient medium for transportation of pathological material containing animal necrobacteriosis excitor // 2640251
FIELD: veterinary medicine.SUBSTANCE: method involves obtaining of a Staphylococcus aureus filtrate by sowing the daily culture of Staphylococcus aureus in meat-peptone broth with addition of 10% sodium chloride and culturing in a thermostat at a temperature of 37°C for 8-10 days, followed by inactivation in a water bath for 30 minutes at a temperature of 90-95°C. The supernatant is separated by centrifugation at 1000 rpm for 20 minutes and filtered through a Seitz filter.EFFECT: increased viability of the neobacteriosis excitor and increased effectiveness of primary diagnostics and treatment and preventive measures.13 dwg, 6 tbl, 5 ex
Biologically-active preparation, accelerating mineralization of remains, and acceleration method of remains mineralization by biologically active preparation (versions) // 2639729
FIELD: ecology.SUBSTANCE: biologically-active preparation that accelerates the mineralization of the remains includes a mixture of spores of non-pathogenic bacteria belonging to the Bacillus species, and additionally includes a moisture retention component - a clay nontoxic mineral, capable of swelling at a hydration by a factor of 14-16, nutrients, vitamins, trace elements and a source of thermal activation to support the vital activity of non-pathogenic bacteria in the form of potassium peroxide K2O2 and/or sodium peroxide Na2O2. Methods of the remains mineralization acceleration with the help of a biological preparation.EFFECT: inventions allow to accelerate the remains mineralization at low temperatures and under unfavourable hydrological conditions, and also contribute to the improvement of the environment.13 cl

Xanthomonas campestris bacteria strain - xanthan producer // 2639557
FIELD: biotechnology.SUBSTANCE: Xanthomonas campest is 2017 strain, which has the ability to produce xanthan, was deposited in the Russian National Collection of Industrial Microorganisms under the registration number of RNCIM V-12968. The strain can be used in medicine, food, cosmetic and pharmaceutical industries.EFFECT: invention allows to increase the yield of xanthan.1 dwg, 6 ex

Lactopsillus rhamnosus bacteria suitable for eating // 2639547
FIELD: biotechnology.SUBSTANCE: Lactobacillus rhamnosus strain, intended for removal and/or neutralisation of toxic products, is deposited in CNCM under registration number I-4719. A method for obtaining of a cell fraction comprising cells culturing and Lactobacillus rhamnosus strain CNCM I-4719 isolation from the culture medium is proposed. A fraction of Lactobacillus rhamnosus cells and a composition containing a Lactobacillus rhamnosus strain CNCM I-4719, suitable for use by direct introduction into a food product, are proposed.EFFECT: increased effectiveness of animals and humans detoxification.6 cl, 4 dwg, 1 tbl, 4 ex
Sinorhizobium fredii nodule bacterial strain for manufacturing of bacterial soya fertiliser // 2639516
FIELD: biotechnology.SUBSTANCE: invention refers to biotechnology. A strain of nodule bacteria Sinorhizobium fredii AmB-17 is deposited in the VKM under the registration number VKM B-3152D. The strain can be used in the manufacture of bacterial fertiliser for soy.EFFECT: invention improves soybean plant productivity.1 tbl
ethod of sylvinite constructions operation efficiency estimation // 2639511
FIELD: biotechnology.SUBSTANCE: method for evaluating the effectiveness of sylvinite constructions is proposed. The proposed method is that Petri dishes with blood agar and S. aureus culture are placed in the sylvinite and control rooms for a time equal to the duration of the halotherapy procedure for a period of 1 to 18 hours, then incubated for 24 hours at 37°C, after which a measurement diameter of the grown colonies is carried out, and when the diameter of the colonies is reduced in comparison with the control, a conclusion about the effectiveness of the functioning of sylvinite structures is made.EFFECT: method makes it possible to carry out a comprehensive evaluation of the effectiveness of sylvinite constructions taking into account all the factors of the internal medical environment.6 tbl, 3 ex
Escherichia coli strain - producer of modular nanoconveyor for delivery of pharmaceutical agents to nuclei of target cells // 2639501
FIELD: biotechnology.SUBSTANCE: invention relates to E. coli RosettaTM strain (pRARE2, pR752), RNCIM B-11308. This strain is a producer of a modular nanoconveyor to increase the specificity and effectiveness of antitumor agents of 6His-HMP-NLS-DTox-(Gly-Ser)5-EGF. The present invention makes it possible to obtain a modular 6His-HMP-NLS-DTox-(Gly-Ser)5-EGF nanoconveyor.EFFECT: increase in the specificity and effectiveness of antitumor agents.2 dwg, 2 ex

Recombinant pichia pastoris yeast strain - producer of secreted xyloglucanase from aspergillus cervinus fungi and method for xyloglucanase microbiological synthesis based on this strain // 2639248
FIELD: biotechnology.SUBSTANCE: method for microbiological synthesis of xyloglucanase encoded by the said gene is provided by culturing of the said recombinant strain under aerobic conditions in a suitable nutrient medium to maximize accumulation of the desired product.EFFECT: thermostable xyloglucanase with optimal activity.2 cl, 1 dwg, 4 ex

L-lysin-producing coryneformic bacterium with inactive ltbr genome and method for obtaining of l-lysin using this bacterium // 2639247
FIELD: biotechnology.SUBSTANCE: invention is an L-lysine-producing bacterium belonging to the Corynebacterium genus or the Brevibacterium genus and modified so that the ltbR gene encoding the transcriptional regulator is inactivated. This invention also discloses a process for L-lysine production by the said bacterium by culturing under suitable conditions.EFFECT: increased level of products.3 cl, 1 tbl, 4 ex

Recombinant dna plasmid pclcrfp, coding fluorescent protein rfp production, for determination of bioavailable chlorated catechohols, their analogs and heavy metals // 2639237
FIELD: biotechnology.SUBSTANCE: said pClcRFP plasmid encodes production of a hybrid fluorescent protein RFP to determine bioavailable chlorinated catechols, their analogs and heavy metals. The nucleotide sequence of pClcRFP plasmid is shown in Fig. 2. This plasmid is derived from the pGEM-T vector, with a promoter region of clc operon (421 bp), full-length clcR (912 bp) gene, clcR-clcA intergenic space (222 bp), the initial part of coding sequence clcA (252 bp) and red fluorescent protein rfp gene introduced under the T7 promoter. This rfp gene is combined with this part of the coding sequence of clcA gene in the reading frame.EFFECT: invention allows to detect bioavailable chlorinated catechols, their analogs and heavy metals in natural media.5 dwg, 2 ex

icroorganism, having high productivity in connection of dairy acid, and a method of obtaining dairy acid with using this microorganism // 2636467
FIELD: biotechnology.SUBSTANCE: modified microorganism is characterized by the fact that it reduces the activity of pyruvate decarboxylase, increases the activity of aldehyde dehydrogenase and acetyl-CoA synthetase, and introduces lactic acid dehydrogenase. As a result of these modifications, this microorganism is able to produce lactic acid in high yield. The invention also relates to a process for the production of lactic acid. The method comprises culturing said microorganism and isolating from the culture medium lactic acid produced by culturing this microorganism.EFFECT: production of lactic acid in high yield.6 cl, 1 dwg, 14 tbl, 10 ex

Advanced methods for recombinant proteins isolation // 2636353
FIELD: biotechnology.SUBSTANCE: method comprises culturing of a prokaryotic host cell producing a recombinant protein, isolation of the said recombinant protein, and purification to a filtered storage preparation (FBS). The step of isolation of the said recombinant protein comprises homogenization. This method is characterized by the level of dissolved oxygen in recovery of the said recombinant protein being maintained at 75% or more before homogenization and at 50% or more after homogenization. The invention also relates to a method for producition of a recombinant protein comprising prokaryotic host cell culturing with deletion of the menE gene. The method comprises culturing of the said prokaryotic host cell producing a recombinant protein, recombinant protein isolation and purification to FBS. The methods of the invention are characterized by the obtained FBS containing no detectable amounts of the recombinant protein adduct and 1,4-dihydroxy-2-naphthoate (DHNA) according to the data of ion-exchange chromatography (IEC) at 310 nm.EFFECT: invention allows to obtain a recombinant protein, the filtered preparation of which does not contain brown adduct and meets technical requirements.18 cl, 12 dwg, 3 tbl, 5 ex
Probiotic strains for application in osteoporosis treatment or prevention // 2636027
FIELD: biotechnology.SUBSTANCE: application of the probiotic strain Lactobacillus paracasei 8700:2, DSM 13434 and the use of the strain Lactobacillus paracasei 8700:2, DSM 13434 in combination with at least one of Lactobacillus plantarum strains for osteoporosis treatment or prevention, bone tissue treatment or prevention, for Ca2+ ions absorption increase in a mammal. Application of the probiotic strain Lactobacillus paracasei 8700:2, DSM 13434 in combination with Lactobacillus plantarum HEAL 9, DSM 15312 and Lactobacillus plantarum HEAL 19, DSM 15313 for osteoporosis treatment or prevention, bone tissue treatment or prevention, for Ca2+ ions absorption increase in a mammal is proposed.EFFECT: reduced cortical bone loss in treatment of postmenopausal osteoporosis.24 cl, 6 dwg, 3 tbl, 4 ex

Gardnerella vaginalis (variants) strains, potentially important for bacterial vaginosis diagnostics, and strain including collection them for bacterial vaginosis diagnostics // 2636005
FIELD: biotechnology.SUBSTANCE: strains of Gardnerella vaginalis VKM B-3001D (genotype I) and Gardnerella vaginalis VKM B-3003D (genotype III), which have a pronounced pathogenic potential, an ability to colonize vaginal mucosa in women of reproductive age and cause severe clinical symptoms of bacterial vaginosis, and strain of Gardnerella vaginalis VKM B-3002D (genotype II), which has less pathogenic potential and colonizing ability, which is associated with asymptomatic bacterial vaginosis, and a collection containing these strains, are proposed. The collection is intended for selection of site-specific primers when creating a diagnostic PCR panel that allows to identify all three genotypes and evaluate the pathogenic potential of Gardnerella vaginalis directly in vaginal discharge specimens.EFFECT: increased accuracy of the vaginosis diagnosis.4 cl, 3 dwg, 3 ex, 1 tbl
icrobial preparation for hydrocarbon contamination disposal // 2634403
FIELD: biotechnology.SUBSTANCE: microbial preparation for hydrocarbon contamination disposal in the water bodies areas, shoreline at temperatures of +4 to +20°C and salinity up to 30 g/l. The preparation contains a biomass of psychroactive microorganisms: Psychrobacter maritimus ARC 7 strain and Arthrobacter rhombi ARC 15 strain (VKPM B-12347 and 15 VKPM As-1989 respectively) at a volume ratio of 1:1 with an allowable deviation from the specified value of not more than 10%.EFFECT: invention allows to increase the degree of water bodies areas and shoreline purification from oil and oil products at a temperature.2 ex
icrobial preparation for hydrocarbon contamination disposal // 2634399
FIELD: biotechnology.SUBSTANCE: microbial preparation for hydrocarbon contamination disposal in the water bodies areas, shoreline, at temperatures of +4 to +20°C and salinity up to 30 g/l. The preparation contains a biomass of psychroactive microorganisms: Psychrobacter cibarius ARC 9 strain, Cobetia marina ARC 10 strain and Nocardia coeliaca ARC 12 strain (VKPM B-12348, VKPM B-12349 and VKPM Ac-1990 respectively) at a volume ratio of 1:1:1 with an allowable deviation from the specified value of not more than 10%.EFFECT: invention allows to increase the degree of water bodies areas and shoreline purification from oil and oil products at a temperature.2 ex
icrobial preparation for hydrocarbon contamination disposal // 2634397
FIELD: biotechnology.SUBSTANCE: microbial preparation for hydrocarbon contaminants disposal in the water bodies areas, shoreline at temperatures of +4 to +20°C and salinity up to 30 g/l. The preparation contains a biomass of psychroactive microorganisms: Halomonas boliviensis ARC 3 strain, Psychrobacter fozii ARC 5 strain and Leucobacter aridicollis ARC strain (VKPM B-12344, VKPM B-123456 and VKPM As-1992 respectively) at a volume ratio of 1:1:1 with an allowable deviation from the specified value of not more than 10%.EFFECT: invention allows to increase the degree of water bodies areas and shoreline purification from oil and oil products at a temperature.2 ex
icrobial preparation for hydrocarbon contamination // 2634396
FIELD: biotechnology.SUBSTANCE: microbial preparation is proposed for the utilisation of hydrocarbon contamination in water areas of water bodies, shorelines, at temperatures from +4 to +20°C and salinity up to 30 g/l. The preparation contains biomass of psychoactive microorganisms: Arthrobacter rhombi strain ARC 8, strain of Psychrobacter cibarius ARC 13 and strain Salinibacterium amurskyense ARC 14 (VKPM Ac-1987, VKPM B-12351, VKPM As-1993 respectively) in a volume ratio of 1:1:1 with allowable The deviation from the specified value is not more than 10%.EFFECT: invention allows to increase the degree of cleaning of water areas of water bodies and shoreline from oil and oil products.2 ex
Strains of nematophage fungus arthrobotrys oligospora infecting eggs and larvae of cyst-forming golden nematode globodera rostochiensis in cysts // 2634390
FIELD: biotechnology.SUBSTANCE: strain of the nematophage fungus Arthrobotrys oligospora T-15, which has the ability to germinate inside cysts and infect the eggs, as well as the nematode larvae in them, destroying their contents, is deposited in the All-Russian Collection of Industrial Microorganisms under the registration number VKPM F-1303. The strain of nematophage fungus Arthrobotrys oligospora can be used to produce biological preparations for plant protection.EFFECT: increased potato yield.4 dwg, 3 ex

icrobacterium strain, compositions and methods to combat fusariosis // 2634386
FIELD: biotechnology.SUBSTANCE: isolated strain of the Microbacterium NRRL B-50470 species having suppressor activity against fusariosis, compositions and methods using this strain are proposed. A method for prevention of fusariosis development involves growing a microbial strain or its culture in a growth medium or plant soil. The treatment method against fusariosis development comprises application of an effective amount of a microbial strain or a culture thereof to the plant or its environment. A seed that is resistant to Fusarium fungus infection, coated by a composition comprising the strain, and a grain plant resistant to Fusarium fungus infection, artificially infected with the strain or its culture, are also proposed.EFFECT: inventions are effective in controlling fusariosis of cereal plants, such as wheat and barley.20 cl, 7 tbl, 11 ex
icrobial preparation for hydrocarbon contamination // 2633690
FIELD: biotechnology.SUBSTANCE: microbial preparation is proposed for the utilisation of hydrocarbon contamination in water areas of water bodies, shorelines, at temperatures from +4 to +20°C and salinity up to 30 g/l. The preparation contains the biomass of psychoactive microorganisms: Nocardia coeliaca ARC 1 strain, Cobetia marina ARC 11 strain and Arthrobacter rhombi ARC 16 strain (VKPM Ac-1991, VKPM B-12350, VKPM As-1988, respectively) in a volume ratio of 1:1:1 s The permissible deviation from the specified value is not more than 10%.EFFECT: increasing the degree of cleaning water areas and shorelines from oil and oil products.2 ex
Amycolatopsis orientalis strain - dimethylvancomycin antibiotic producer and method for antibiotic production // 2633511
FIELD: pharmacology.SUBSTANCE: group of inventions is claimed that can be used for production of antibacterial dimethylvancomycin sulfate antibiotic, effective for treatment of infections caused by gram-positive pathogens, including methicillin-resistant and anaerobic ones. Amycolatopsis orientalis strain, the producer of dimethylvancomycin deposited in VKPM under the registration number VKPM As-1984, and a method fordimethylvancomycin production are proposed. The method for dimethylvancomycin preparation involves cultivation of the Amycolatopsis orientalis strain VKPM-As-1984, antibiotic sorption from the native solution on the KB-2 (Na+) cation exchange, antibiotic desorption, eluate neutralisation with the KU-2-20 (H+) cation exchanger and antibiotic sorption (pH 6.8) on Amberlite CG-25 (NH4+) followed by elution of dimethyl vancomycin (0.05-0.3) n. with ammonia solution. The aqueous solution of the antibiotic is concentrated and precipitated with acetone.EFFECT: increased degree of purification.2 cl, 2 tbl, 2 ex

ethod for fungal spores packaging in modified atmosphere to increase fungi storage life // 2631826
FIELD: biotechnology.SUBSTANCE: method for fungal spores packaging, fungal spores packed by the above method, and a sealed package with viable fungal spores. The method includes reduction of the initial water activity in spores to less than 0.3, spores placement in a gas-tight and water vapour-tight packaging, spores storage in the package at a temperature between 15 and 25°C. The sealed package includes viable fungal spores, medium surrounding the fungal spores, oxygen absorption agent and moisture absorption agent in its interior. At that, relative humidity in the medium after spores placement in the package is reduced, so that the water activity in the spores is less than 0.1 in two days at a temperature between 15 and 25°C.EFFECT: increased period of maintenance of fungal spores viability.14 cl, 2 dwg, 6 tbl, 8 ex
ethod for cultivation of phaffia rhodozyma yeast cells for production of protein-vitamin additive containing astaxanthin carotinoid // 2631803
FIELD: biotechnology.SUBSTANCE: method for Phaffia rhodozyma yeast strains cultivation to obtain a protein-vitamin additive with astaxanthin. The method comprises sequentially growing of Phaffia rhodozyma yeast strains on an agar medium and in seed flasks on medium with starch fermentolysate, corn extract and salts, transfer of the obtained material to fermenters. Cultivation in fermenters is carried out in the absence of light energy, and inoculum cultivation carried out with a 10-fold increase in the volumes of fermenters. Singlet oxygen is used to induce carotinogenesis.EFFECT: possibility of usage of standard fermentation equipment, aseptic conditions of the process, industrial process implementation and minimized cost of lighting.2 cl, 10 ex
ethod for obtaining of nutrient medium for animal necrobacillosis pathogen identification // 2631795
FIELD: veterinary medicine.SUBSTANCE: method involves obtaining of a staphyococcus cultures filtrate by growing staphylococcus cultures on meat-agarone agar, followed by culturing in a thermostat at 37oC for 8-10 days. The staphylococcus culture is inactivated in a water bath at a temperature of 90-95oC for 30 min. It is centrifuged for 20 minutes, followed by supernatant separation and additional filtration through a Seitz filter.EFFECT: shortened period of seed cultivation and increased sensitivity of bacteriological examination.25 dwg, 4 tbl, 5 ex

ethod for trib-2mut recombinant elastromerial domain, production, gene-engineering pgdtrib2mut construction, determining trib-2mut biosynthesis in e.coli cells, e.coli m15/pgdtrib2mut producing strain and method for polymer material obtaining based on this protein // 2631004
FIELD: biotechnology.SUBSTANCE: based on the minimal recombinant elastomeric motif (tribolin-1, Trib-1mut) insect resilinTribolium castaneum (tribolin-2, TRib-2mut), a protein structure is obtained recombinantly, which is used in aggregation methods to form the basis of a biomatrix network.EFFECT: invention allows to obtain polymeric frame biomaterial for tissue engineering.5 cl, 2 dwg, 2 tbl, 4 ex

Compositions and methods relevant to mutant toxin from clostridium difficile // 2630671
FIELD: biotechnology.SUBSTANCE: polypeptide that induces formation of neutralizing antibodies to Clostridium difficile toxins, is characterized by an amino acid sequence represented by any of SEQ ID NO: 4 or SEQ ID NO: 6. This invention also relates to an immunogenic composition comprising an isolated polypeptide. The invention also relates to a recombinant Clostridium difficile cell. This recombinant cell is designed to express the isolated polypeptide. The invention relates to methods for Clostridium difficile culturing and for preparation of the said isolated polypeptide.EFFECT: invention allows to obtain an isolated polypeptide and an immunogenic composition that cause formation of neutralizing antibodies to Clostridium difficile toxins.39 cl, 59 dwg, 71 tbl, 46 ex
Streptomyces globisporus k-35/15 strain as means for plants protection against harmful insects - phytophages // 2630661
FIELD: biotechnology.SUBSTANCE: strain of Streptomyces globisporus K-35/15 actinomycete, possessing entomocidal and acaricidal properties, is deposited in the Departmental Collection of Useful Microorganisms of Agricultural Purpose of FSBSI All-Russian Research Institute of Agricultural Microbiology under the registration number RCAM 04205 and can be used to protect plants from harmful insects - phytophagous, including colorado beetle, spider mites, aphids.EFFECT: invention improves agricultirual plant productivity.6 tbl, 2 ex

Compositions, method and kits for mucosal immune system stimulation // 2630299
FIELD: biotechnology.SUBSTANCE: nutritional compositions for stimulating mucosal immune system, method of manufacturing and applying them are proposed. The composition comprises a milk or cereal product fermented with probiotic bacterium Lactobacillus paracasei (CBA L74) BCCM/LMG LMG P-24778. Alternatively, the composition comprises L. paracasei LMG P-24778 in amount of from about 1×102 CFU/g to about 1012 CFU/g of bacteria on 1 gram of dry mass and a physiologically acceptable carrier. The method of manufacturing the composition comprises combining the food product with an effective amount of Lactobacillus paracasei LMG P-24778 and incubating the mixture at a temperature and for a time sufficient for fermentation. Nutritional kits are proposed, comprising compositions based on L. paracasei LMG P-24778 or fermented milk or grain product and one or more items, selected from group consisting of a packaging material, a package insert including instructions for use, a sterile liquid, and a sterile container.EFFECT: proposed compositions of the invention may be administered to a patient having gastrointestinal disorders or the risk their development, which refers to an inadequate development of immune system, infection or disease, to stimulate immunomodulatory reaction in mucosal immune system.30 cl, 34 dwg, 20 ex

ethod for detection of copper ions in the environment and biosensor for its implementation // 2628704
FIELD: biotechnology.SUBSTANCE: strain of bacteria Escherichia coli BL21 DE3 pClcRFP is suggested for detection of copper ions, biosensor and method for detecting copper ions in the liquid medium under analysis. The biosensor includes a housing with microchannels for the liquid medium under analysis, inside of trap microchannels in form of a series of micropillars with immobilized agarous spheres. Moreover, the agarose spheres contain genetically modified Escherichia coli BL21 DE3 pClcRFP bacterial cells, which are capable of synthesizing the fluorescent protein RFP. The method is carried out by means of the above biosensor and includes immobilizing bacterial Escherichia coli BL21 DE3 pClcRFP cells into agarous spheres with diameter of 50 to 70 mcm, placing them in biosensor traps, feeding the analyzed liquid medium into biosensor, measuring the glow of the agarous spheres, processing and comparing the results with the control ones.EFFECT: inventions provide detection of copper ions in medium and an assessment of contamination degree using a small sample volume and a small amount of genetically modified bacterial cells necessary for the detection of one sample.5 cl, 5 dwg, 3 ex
Biosorbent for soil and water purification from oil and oil products // 2628692
FIELD: biotechnology.SUBSTANCE: preparation has both adsorption capacity due to hydrophobic peat-mineral sorbent, and oil-destructive activity due to microorganisms immobilized on the sorbent. The biosorbent consists of a hydrophobic sorbent based on peat and association of Rhodococcus erythropolis VKPM AS-1660 and Rhodococcus sp. strains VKPM AC-1260 in a ratio of 1:1 by volume. The strains are immobilized on the sorbent by aerosol as part of a bioemulsion, which contains a strains association, vaseline oil, an emulsifier and a mineral additive. Biosorbent features are: aerosol method of bio-emulsion application to the sorbent; prolonged action; provided biodegradation of sorbed oil and oil products at a temperature of plus 2°C to plus 32°C; low desorption of sorbed oil and oil products, no need for additional measures for sorbed oil disposal.EFFECT: invention allows to improve the quality of soil and water surface purification from oil and oil products.3 tbl, 2 ex

Improved work of poultry farm // 2628691
FIELD: biotechnology.SUBSTANCE: composition, a method for poultry farm environment improvement, a method for ammonia formation and urease enzyme inhibition reduction, a method for pathogenic bacteria level reduction, a method for pest control in litter for poultry, a method for pododermatitis prevention in birds. The composition comprises at least one Bacillus subtilis strain in an amount of 1×104 Cfu/ml to 1×1013 Cfu/ml, a bioactive surfactant (surfactin or lichenisin, or fengicin, or iturin, or a mixture thereof) in an amount of 2 mg/l to 7000 mg/l, a surfactant (alkylbenzenesulfonic acid or alkylbenzene sulfonate). The methods comprise application of the proposed composition to the poultry litter.EFFECT: improved performance of poultry farms.18 cl, 1 dwg, 5 tbl, 15 ex
icroencapsulated bacterial consortium for gluten degradation in fermented dough and method for obtaining said fermented dough // 2628314
FIELD: food industry.SUBSTANCE: microencapsulated bacterial consortium for gluten degradation contains Lactobacillus plantarum ATCC 8014, Lactobacillus sanfranciscensis ATCC 27652 and Lactobacillus brevis ATCC 14869, encapsulating agents, prebiotics selected from the group including polydextrose, inulin and agave syrup, and trehalose in combination with a proteolytic enzyme of bacterial origin and a proteolytic enzyme of fungal origin commonly used for baking. The method for obtaining a consortium for gluten degradation comprises the stages of separately reacting each strain of lactic acid bacteria, separately cultivating each activated strain in a liquid culture medium until each strain reaches a predetermined concentration, removing for each strain the culture medium excess for concentrating the microorganisms, preferably by centrifugation, with obtaining granules, separately resuspending the granules for each strain in a suspension of the saline solution and adjusting to a predetermined volume, mixing the required amounts of each strain and adjusting to a final volume, dissolving the encapsulating agents in water at suitable proportions of dissolved dry substances from 20 to 30%, adding prebiotic and trehalose, inoculating the mixture of the three strains of lactic acid bacteria with obtaining approximately 1010 CFU of said mixture of three strains of lactic acid bacteria per gram of the encapsulated powdery substance, and spray drying with the inlet temperature of 110 to 160°C and the outlet temperature of 60 to 80°C, at a feed rate of 20 to 50 ml/min. Invention also relates to fermented dough, the method for its obtaining, and sweet baked products obtained from the fermented gluten-free dough.EFFECT: group of inventions allows to obtain a consortium which is stable and quickly activated.17 cl, 4 tbl, 6 ex
ethod for assessment of cholera vibrio biofilms sensitivity to antibacterial drugs // 2628098
FIELD: biotechnology.SUBSTANCE: method is proposed for assessment of cholera vibrio biofilms sensitivity to antibacterial drugs, including biofilm production on glass cylinders. Cylinders with the resulting biofilm are placed on agar in a Petri dish, antibacterial drug solutions at different concentrations are added in the middle of the cylinder. The results are taken into account after 18-20 h of incubation at 37°C by the presence or absence of cholera vibrios growth in the cylinders prints. Cholera vibrios biofilm sensitivity to antibacterial drugs is assessed by the activity index calculated by the formula: where AI is the antibacterial drug activity index; MICs is the maximum MIC of this antibacterial drug for sensitive strains in accordance with MUK 4.2.2495-09; MICb is MIC value of this antibacterial drug with respect to the biofilm of the strain under study. Biofilm sensitivity of the strain under study is directly proportional to the AI index.EFFECT: accuracy of evaluation of cholera vibrio strains biofilm sensitivity to an antibacterial drug.2 dwg, 4 tbl, 2 ex
Bacillus megaterium 2-06-ts1 microorganisms strain as photosynthesis and plant growth energy activator // 2627608
FIELD: biotechnology.SUBSTANCE: Bacillus megaterium 2-06-TS1 bacterial strain is deposited in the Russian National Collection of Industrial Microorganisms FGUPGosNIIGenetika under accession number VKPM B-12402.EFFECT: invention improves the energy of seed germination, plant germination, increases root growth, as well as chlorophyll b, carotenoids and the total amount of photosynthesis pigments in plant seedlings.3 tbl
ethod for water surface purification from oil pollution // 2627598
FIELD: biotechnology.SUBSTANCE: method comprises microbial preparation application to a water body; the preparation is based on a consortium of Acinetobacter sp. IB DT 5.1/1 and Ochrobactrum sp. IB DT 5.3/2 microorganisms with a titer of at least 108 CFU/ml in the culture fluid. The purification process is carried out by water body aeration.EFFECT: invention allows to reduce time and improve the efficiency of water bodies purification from oil pollution.2 ex

Bacillus pumilus strain and method for obtaining amikumacin a antibiotic with its application // 2627187
FIELD: biotechnology.SUBSTANCE: method for amikumacin obtaining involves cultivation of a Bacillus pumilus bacteria strain VKPM B-12548 on a liquid nutrient medium containing sucrose, glucose, yeast extract, K2HPO4, NaCl, MgSO4, (NH4)2SO4, FeSO4×7H2O, MnCl2×4H2O, CaCO3 and tap water at a predetermined ratio of components, followed by cultivation for 40-42 hours under aerating conditions. Cells from the culture liquid are precipitated by centrifugation to obtain a supernatant. Amberlite XAD-2 sorbent is added to the supernatant, followed by elution of amikumacin A with n-butanol-acetone-water system at a ratio of 1:1:1.EFFECT: invention allows to increase the yield of amikumacin A antibiotic.2 cl, 3 dwg, 1 tbl, 1 ex

Aneurinibacillus migulanus strain and its application for gramicidin s production // 2627182
FIELD: biotechnology.SUBSTANCE: invention can be used to produce gramicidin S using the strain of the Aneurinibacillus migulanus VKPMV-10212 producer.EFFECT: resulting strain allows production of gramicidin S in high yield.2 cl, 2 dwg, 2 ex

Bacterium strains lactobacillus rhamnosus, with a wide spectrum of antagonistic activity relating to pathogenic and conditionally pathogenic microorganisms // 2627166
FIELD: biotechnology.SUBSTANCE: strain of bacteria Lactobacillus rhamnoses 24 ds, which has antagonistic activity against pathogenic and opportunistic microorganisms, including against yeast fungi of the genus Candida, deposited in the All-Russian Collection of Industrial Microorganisms under the registration number VKPM V-12596 and can be used In the production of probiotic bacterial preparations, biologically active food additives, fermented and unfermented food products.EFFECT: improved strain properties.1 dwg, 7 tbl, 2 ex

Bacterium strains lactobacillus rhamnosus 7 ds, with a wide spectrum of antagonistic activity relating to pathogenic and conditionally pathogenic microorganisms // 2627165
FIELD: biotechnology.SUBSTANCE: strain of bacteria Lactobacillus rhamnosus 7ds, which has antagonistic activity against pathogenic and opportunistic microorganisms, including those with yeast fungi of the genus Candida, was deposited in the All-Russian Collection of Industrial Microorganisms under the registration number VKPM V-12598. The strain can be used in the production of probiotic bacterial preparations, dietary supplements, fermented and unfermented foods.EFFECT: improved strain properties.1 dwg, 7 tbl, 2 ex
 
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