Indexing scheme associated with subclasses c12c-c12q and or c12s, relating to micro-organisms (C12R)

C   Chemistry; metallurgy(311501)
C12R              Indexing scheme associated with subclasses c12c-c12q; or c12s, relating to micro-organisms(2724)
Biological product for stimulating the growth of plants and their protection against phytopathogens on the basis of strains of trichoderma, strains of trichoderma for the production thereof (variants), method for producing a biological product on the basis of such strains // 2607785
FIELD: biotechnology.SUBSTANCE: group of inventions relates to biotechnology and can be used to create bioprotection of plants against phytopathogens and to stimulate their growth. Group of inventions comprises the strains of the fungus of Trichoderma longibrachiatum species (3 options); biological product for stimulating the growth of plants and their protection against phytopathogens on the basis of these strains and the method for producing a biological product. Strains of the Trichoderma longibrachiatum fungus are deposited at the ARRIAM FSBSI under registration numbers: RCAM 03324, RCAM 03323, RCAM 03325 respectively. Biological product contains spores and fragments of mycelium of above strains from 2×109 to 4×109 CFU/cm3; has antagonistic activity against phytopathogenic fungi of the Fusarium and Cladosporium species. Method of producing a biological product involves a two-stage joint cultivation of strains in a quantitative ratio of 1:1:1 consistently on two nutrient media for 4–5 days. This group of inventions provides increase in seed germination on 4–15 % and the increase of linear sizes of plants on 0.5–24 %.EFFECT: increased seed germination and the increase of linear sizes of plants.5 cl, 2 tbl, 5 ex

Strain of lactic acid bacteria, drug, food product, beverage, as well as fodder containing strain of lactic acid bacteria // 2607370
FIELD: biotechnology.SUBSTANCE: group of inventions relates to biotechnology. Strain Lactobacillus paracasei MCC1849 with high stimulating production of IL-12 activity.EFFECT: strain is used as ingredient of medicinal agent for immune stimulation, agent against influenza virus, food product, beverage, fodder and agent stimulating production of IL-12.8 cl, 2 dwg, 3 tbl, 3 ex
Strain of microorganisms achromobacter spanius 10-50-ts2 as agent improving plant resistance to chloride salinity // 2607028
FIELD: biotechnology.SUBSTANCE: invention relates to agricultural biotechnology. Invention represents strain of microorganisms Achromobacter spanius 10-50-TS2, deposited in Russian national collection of industrial microorganisms FGUPGosNIIGenetika under No. B-12405, as agent for increasing resistance of plants in conditions of chloride salinity of soils. Strain of bacteria Achromobacter spanius 10-50-TS2 No. B-12405 increases germination power of seeds by 40 % (p < 0.001), laboratory capacity by 35.2 % (p < 0.001), increases length of germs by 59 %, weight of seedlings in 2 times, increases number of roots by 13.3 %, root mass in 2 times under conditions of chloride salinity.EFFECT: higher resistance of plants.1 cl, 1 tbl
Biotransformation phenylmethyl sulphide in (r)-sulphoxide using immobilized cells gordonia terrae iegm 136 // 2607027
FIELD: biotechnology.SUBSTANCE: invention relates to biotechnology, in particular to production of (R)-phenylmethylsulfoxide. Method involves oxidation of phenylmethylsulfoxide using biocatalyst-immobilized in cryogel of polyvinyl alcohol of cells Gordonia terrae IEGM 136. Process is carried out in mineral medium, containing n-hexadecane in amount not exceeding 0.1 vol. %.EFFECT: invention allows to increase output of the end product and optimize the process of its production.3 cl, 2 dwg, 3 tbl, 8 ex
Dry bacterial starter for production of fermented milk products and production method thereof // 2607023
FIELD: biotechnology.SUBSTANCE: declared group of inventions relates to biotechnology. Method of production of dry bacterial starter for production of fermented milk products involves separate cultivation of strains Streptococcus thermophilus KD7 41 №2 VKPM V-10403 and Streptococcus thermophilus ZL-047 VKPM V-10707. Fermentation of each strain is carried out to produce a liquid biomass. Carried out concentrating of the resulting liquid biomass of each strain, mixing of each strain with a protective medium at a ratio of 1:2–1:4 with further separate drying and mixing the obtained biomass Streptococcus thermophilus KD7 41 №2 VKPM V-10403 and Streptococcus thermophilus ZL-047 VKPM V-10707 in ratio of – 3:7 with producing dry bacterial starter.EFFECT: invention allows to extend storage life of starter in the high content of active living cells of starter and cut the duration of milk fermentation.4 cl, 1 ex
Test strain leptospira of interrogans serogroup icterohaemorrhagiae serovar copenhageni for detection of antibodies to l icterohaemorrhagiae // 2607006
FIELD: biotechnology; medicine.SUBSTANCE: invention relates to medical biotechnology and can be used for detection of antibodies to L. icterohaemorrhagiae. Test strain Leptospira interrogans of Icterohaemorrhagiae serogroup copenhageni serovar with evident antigenic and immunogenic properties is deposited in State collection of pathogenic microorganisms and cell cultures "GKPM-Obolensk" under registration number V-7745.EFFECT: invention provides serological diagnosis of leptospirosis, monitoring of distribution of icterohaemorrhaghic leptospirosis among people and among different species of animals.1 cl, 1 tbl, 2 ex

Lactobacillus mucosae strain for producing fermented food products // 2606770
FIELD: food industry.SUBSTANCE: disclosed are Lactobacillus mucosae for producing fermented food products and a food product containing said strain. Lactobacillus mucosae strain is deposited in CNCM under number I-4429. Strain has capability to reduce permeability of intestinal barrier.EFFECT: invention can be used to alleviate conditions, including dysfunction of intestinal barrier, in particular conditions related to increase in permeability of intestinal barrier.3 cl, 5 dwg, 3 tbl, 5 ex

ethod of producing capsular polysaccharide with pneumococcal serotype // 2606022
FIELD: biotechnology.SUBSTANCE: present invention relates to biotechnology. Method of producing capsular polysaccharide with pneumococcal serotype is disclosed. Bacterial cells are cultured at culture fluid pH in range of 7.0 to 9.4. Then cultivation is stopped at the moment between culture fluid absorption coefficient is constant and absorption coefficient begins to fall. Additional cultivation is performed without pH regulation until culture fluid pH reaches value of 5.5 or less. Then lysing agent is added to obtained culture fluid for cell lysis. Precipitated proteins and cellular debris are removed to obtain purified cell lysate. Capsular polysaccharide is separated and purified from obtained lysate.EFFECT: invention eliminates process of depositing protein by increasing acidity by means of pH regulator, which simplifies process and does not lead to modification of capsular polysaccharide and formation of hazardous substances.13 cl, 6 dwg, 10 tbl, 3 ex
Recombinant plasmid dna pefn877, determining expression of the hybrid polypeptide with properties of 10-th fibronectin domain on the surface of escherichia coli cells, and strain of bacteria escherichia coli bl21(de3)plyss/pefn877 - producer of hybrid polypeptide with properties of 10-th fibronectin domain on the cells surface // 2606014
FIELD: biotechnology.SUBSTANCE: invention relates to biotechnology and can be used for producing the hybrid polypeptide alternative carcass protein 10-th human fibronectin domain. Recombinant plasmid DNA pEFN877 designed on the basis of plasmid pET20b(+) length 3.654 t.p.o. Obtained plasmid pEFN877 comprises hybrid of gene EFN877 with SEQ ID NO: 8, coding amino acid sequences of esterase P. cryohalolentis K5T, 10-th domain of human fibronectin and autotransporter P. cryohalolentis K5T in a single reading frame, arranged in accordance with fig. 1. Bacterial strain E. coli BL21(DE3)pLysS/pEFN877 is obtained by transformation of competent cells E. coli BL21(DE3)pLysS of plasmid DNA pEFN877.EFFECT: invention allows to obtain cell strain E.coli – producer of hybrid polypeptide with properties of 10-th human fibronectin domain on the cells surface.2 cl, 4 dwg, 9 ex

ethod for stimulating growth processes of chlorella microalgae // 2605636
FIELD: biotechnology.SUBSTANCE: invention relates to biotechnology and can be used for improving productivity of cultivation of microalgae Chlorella. Method comprises treating of microalgae Chlorella vulgaris IFR № C-111 by ozone-air mixture with ozone concentration of not more than 7.2 mg/m3 during 6 minutes a daylight on the first and second days after sowing.EFFECT: invention allows to increase output of biomass of microalgae Chlorella vulgaris IFR № C-111.1 cl, 4 dwg, 4 tbl

ethod for producing allergen for differentiating dried purified tuberculine nonspecific reactions for mammals // 2605633
FIELD: veterinary science.SUBSTANCE: invention relates to veterinary microbiology. Method for producing allergen for differentiating dried purified tuberculine nonspecific reactions for mammals is proposed. Method comprises growing Mycobacteria m.Avium strain 2282 (3rd Ranion group), M.scrofulaceum (2nd Ranion group) and M.fortuitum (4th Ranion group) separately on a liquid nutrient medium Soton at temperature of 37-38 °C for 4-8 weeks and their inactivation. Protein from a culture filtrate of each strain is separately deposited with trichloroacetic acid to the final concentration of 6-8 %, followed by dialysis of protein solution, filtration through membrane plates (Immersible CX-10), mixing protein solutions in equal portions by content of activity units (u), filtration, stabilisation and packaging.EFFECT: method provides higher purity of the preparation and higher quality of differential diagnosis of dried purified tuberculin nonspecific reactions for mammals.1 cl, 2 tbl

Recombinant strain of yeast pichia pastoris - producer of the secreted thermostable xyloglucanase coded by synthetic gene, and method for microbiological synthesis of secreted thermostable xyloglucanase based on this strain // 2605629
FIELD: biochemistry.SUBSTANCE: invention relates to biochemistry, genetic engineering and biotechnology, in particular, to strain Pichia pastoris VKPM Y-4269. This is a producer of secreted thermostable xyloglucanase of GH74 class. Said xyloglucanase encoded by synthetic gene corresponding to SEQ ID NO 1. Present invention also relates to a method for microbiological synthesis of xyloglucanase. Above method involves culturing a recombinant microorganisms, containing gene xyloglucanase, in aerobic conditions in a nutrient medium to maximum accumulation of the end product. This nutritional medium includes sources of carbon, nitrogen and mineral additives. Above method is characterised by that is as a producer is used a recombinant strain Pichia pastoris VKPM Y-4269.EFFECT: present invention enables to obtain secretable thermostable xyloglucanase of class GH74 with high output and purity.2 cl, 2 dwg, 3 ex

ethod for obtaining spore culture on basis of bacterial strain bacillus species 1839 // 2605543
FIELD: biotechnology.SUBSTANCE: invention relates to biotechnology, microbiology and can be used for production of spore material of bacteria strain Bacillus sp. 1,839. Method involves inoculation of nutrient medium with bacteria of strain Bacillus sp. 1,839 with further cultivation on it. Biomass is separated by centrifugation at 1,000-1,500 rpm. In obtained bacteria biomass hypertonic phosphate buffer is added, containing potassium chloride, sodium hydrogen phosphate, potassium dihydrogen phosphate and sodium chloride at given ratio. Bacillus sp. 1,839 incubation is performed until formation of spores. For removal of remained vegetative cells thermal treatment is performed by heating obtained precipitate of spores on water bath at least for 10 minutes at 70-90 °C, supernatant is centrifuged and removed.EFFECT: invention simplifies method and allows obtaining spore culture in any bacterial or veterinary laboratories.3 cl, 4 ex

Recombinant chimeric protein sumo3-apoa-i for producing mature human apolipoprotein a-i, pichia pastoris yeast strain - producer of recombinant chimeric protein sumo3-apoa-i and method of producing mature human apolipoprotein a-i // 2605326
FIELD: genetic engineering.SUBSTANCE: to increase yield of mature human apolipoprotein A-I it is produced by enzymatic hydrolysis of SUMO-specific protease SP2 of recombinant chimeric protein SUMO3-apoA-I, synthesised by Pichia pastoris yeast strain. Said strain is constructed by transformation of Pichia pastoris X-33 yeast strain with linearised plasmid DNA pPsecSUMO3-apoA-I. Recombinant chimeric protein SUMO3-apoA-I includes from N-terminal amino acid sequence of ubiquitin-like protein of human SUMO3, fused at C-terminal with amino acid sequence of mature human apoA-I protein.EFFECT: invention can be used for producing mature human apolipoprotein A-I.3 cl, 1 tbl, 5 dwg, 7 ex

Expression system and method of producing non-modified recombinant proteins in escherichia coli using it // 2604796
FIELD: biotechnology.SUBSTANCE: group of inventions relates to biotechnology. DNA construct is disclosed, which codes fused precursor protein, in which auxiliary amino acid sequence is bound to N-end of mature target polypeptide sequence with transition site intended for identification and splitting of hybrid precursor by specific protease to form non-modified protein of interest. Above site represents truncated at N-end by 23 amino acid residues SUMO protein of S. cerevisiae (Smt3), which includes site of splitting with SUMO-hydrolase (Ulp1). On basis of DNA construct coding version of precursor, which comprises glutathione-S-transferase (GST) as auxiliary sequence and negatively charged peptide and human interferon (IFN) α2b as target protein recombinant E. coli strain is obtained. Method of producing non-modified IFN α2b using above E. coli strain is also disclosed.EFFECT: group of inventions enables to obtain target protein with high purity and activity.7 cl, 9 dwg, 2 tbl, 5 ex

Culture medium for growing of bacteria // 2604789
FIELD: microbiology.SUBSTANCE: invention relates to microbiology. Nutrient medium for growth of bacteria is disclosed. Nutrient medium contains pancreatic casein overcook, meat pepsin overcook, heart pancreatic overcook, yeast extract, starch, water, violuric acid and infusion of beef.EFFECT: invention provides simultaneous growth of maximum possible number of bacteria in plated material.7 cl, 6 tbl

ethod of cleaning freshwater ecosystems from oil and oil products at high latitudes // 2604788
FIELD: biotechnology.SUBSTANCE: invention relates to biotechnology. Method of cleaning freshwater ecosystems from oil and oil products is proposed. Method comprises contacting oil hydrocarbons to be decomposed with a biopreparation, consisting of a mixture of oil-oxidising Microbacterium species VKM Ac-2614D, Pseudomonas migulae BKM B-2761D, Rhodococcus erythropolis VKM Ac-2612D, Rhodococcus erythropolis VKM Ac-2611D strains, taken in ratio of 1:1: 1:1. Cell titre in the ready preparation is, at least, 1010 cells per 1 g.EFFECT: method provides high rate of biodegradation of oil in aqueous media in temperature range from 0 to +25 °C, including ice conditions.5 cl, 4 dwg, 7 tbl, 6 ex

Strain of bacteria serratia marcescens m-10 is producer of extracellular non-specific endonuclease with marked antiviral action // 2604183
FIELD: biotechnology.SUBSTANCE: strain is deposited in VKM under number VKM B-2931D. Endonuclease activity of strain to 36 hours cultivation on meat-peptone infusion broth is 28,720 units/ml of cultural liquid.EFFECT: strain of bacteria Serratia marcescens, being a producer of nonspecific bacterial endonuclease.1 cl, 1 tbl, 1 ex

ethod of microalgae biomass flocculation // 2603733
FIELD: biotechnology.SUBSTANCE: invention relates to biotechnology and can be used in municipal (water treatment and wastewater) and agriculture, mining industry, medicine, biotechnological industry. Method of microalgae biomass flocculation from suspending medium is proposed. Method involves successive addition to suspending medium with biomass of microalgae coagulant six-water iron (III) chloride in concentration of 20-40 mg/l and flocculants: polyacrylamide and polyethylene oxide in amount, respectively, of 2-5 mg/l and 1-4 mg/l. Flocculation of biomass of microalgae is carried out, while stirring at temperature of 10-40 °C.EFFECT: invention provides increased rate of deposition of microalgae biomass.8 cl, 3 tbl

Strain escherichia coli bl21(de3)gold/petcypopti - producer of human recombinant cyclophilin a // 2603283
FIELD: biotechnology.SUBSTANCE: invention relates to biochemistry, genetic engineering and biotechnology, namely to strain Escherichia coli BL21(DE3)GoldpETCYPopti. This strain is producer of recombinant human cyclophilin A. Strain is produced by cell transformation of strain Escherichia coli BL21(DE3)Gold by pETCYPopti plasmid. Said plasmid has size of 5858 base pairs and contains fragment XhoI-NdeI of vector pET-22b(+) carrying gene of ampicillin resistance ampR, promoter and terminator of phage T7 RNA-polymerase and polylinker, wherein fragment, carrying gene of human recombinant cyclophilin A, is cloned at sites XhoI-NdeI with size of 498 base pairs, SEQ ID NO: 1.EFFECT: present invention enables to obtain human cyclophilin A with high output and purity.1 cl, 3 dwg, 1 ex
Bioinsecticide for controlling large bee moth // 2603271
FIELD: agriculture.SUBSTANCE: invention relates to agriculture, in particular, to bee keeping, and can be used for controlling large bee moth. Bioinsecticide for large bee moth contains a Bacillus thuringiensis ARRIAM RCAM 00045 strain and filler, for example, NaCl, in a given ratio of components.EFFECT: invention enables to recover bee families of large bee moth and preventive treatment of injuries of bee families of large bee moth larvae during entire honey harvesting season improves safety of bee families.1 cl, 2 ex

ethod for detoxification of white phosphorus using microorganism strain trichoderma asperellum vkpm f-1087 // 2603259
FIELD: biotechnology.SUBSTANCE: invention relates to biotechnology, specifically to a method for detoxification of white phosphorus in contaminated soil. Decontamination is performed by treatment of white phosphorus-contaminated soil with a strain of Trichoderma asperellum VKPM F-1087. Method enables detoxification of soil containing white phosphorus in concentration up to 1.0 wt%, exceeding the maximum allowable concentration 5,000 times.EFFECT: method enables detoxification of soil.1 cl, 1 dwg
Strain of bacteria desulfovibrio species vk-9 for purification of acidic wastewater from heavy metal ions // 2603249
FIELD: microbiology.SUBSTANCE: invention relates to industrial microbiology. Bacterial strain Desulfovibrio sp. VK-9 has high resistance to high concentrations of copper ions (up to 125 mg/l), nickel ions (up to 250 mg/l), cobalt ions (up to 350 mg/l) and cadmium ions (up to 60 mg/l) and can be used for purification of acidic wastewater from machine building, instrument-making, electrical, mining enterprises, electroplating industries, as well as for acidic mine water purification from high concentrations of copper ions and other heavy metals. Strain Desulfovibrio sp., is deposited in Russian National Collection of microorganisms of Institute of biochemistry and physiology of microorganisms RAS IBFM under registration number VKM V-3000D.EFFECT: invention allows to increase degree of wastewater purification from heavy metal ions.1 cl, 2 tbl, 4 ex

Strain of microalgae chlorella vulgaris beyerinck - producer of mixture of aromatic substances similar to resinoid of oakmoss // 2603099
FIELD: biotechnology.SUBSTANCE: invention relates to biotechnology, applied microbiology and can be used for use as a producer of aromatic compounds. Strain of microalgae Chlorella vulgaris Beyerinck SMP-1802153, capable of synthesis of a mixture of aromatic substances similar to resinoid of oakmoss, deposited in collection of microalgae IPPAS under registration number Chlorella vulgaris Beyerinck IPPAS C-2019.EFFECT: invention increases output of mixture of aromatic substances similar to resinoid of oakmoss.1 cl, 1 tbl, 3 ex

Recombinant plasmid dna pndctr1 coding hybrid polypeptide gst-ndctr1, escherichia coli bl21 (de3)/pndctr1 bacteria strain - producer of hybrid polypeptide gst-ndctr1 and hybrid polypeptide gst-ndctr1, making chelates of copper, silver and platinum ions // 2603092
FIELD: biotechnology.SUBSTANCE: invention relates to biotechnology and can be used for recombinant production of CTR1. Method comprises producing plasmid DNA pNdCTR1 coding hybrid polypeptide GST-NdCTR1, consisting of the N-terminal polypeptide fragment of glutathione-S-transferase (GST), connected via thrombin hydrolysis site with the polypeptide fragment of the N-terminal domain of a high-affinity human copper CTR1 (NdCTR1) importer. Size of the plasmid DNA is 5149 bps, the plasmid DNA comprises BamHI-XhoI - pGEX-4T-1 fragment with size of 4951 bps and SLC31A1 gene fragment with size of 198 bps, encoding the N-terminal domain of human CTR1 protein. Nucleotide NdCTR1 sequence and GST sequence are in the same reading frame. E.coli BL21(DE3) cells are transformed with the produced DNA pNdCTR1 to obtain an E.coli BL21 (DE3)/pNdCTR1 producer strain.EFFECT: invention allows producing hybrid polypeptide GST-NdCTR1 making chelates of copper, silver and platinum ions.3 cl, 4 dwg, 1 tbl, 5 ex
Strain of lactic bacteria enterococcus durans - producer of lactic acid and antibiotic substances // 2603087
FIELD: biotechnology.SUBSTANCE: invention relates to biotechnology. Disclosed is a strain of Enterococcus durans kos 37 b No. 19 with high antagonistic capacity with respect to opportunistic and pathogenic microflora. Strain is deposited in VKPM under registration number VKPM V-11960. Strain of Enterococcus durans VKPM V-11960 can be used in food industry, as well as a base for producing a probiotic starter.EFFECT: invention reduces duration of fermentation of milk.1 cl

Isolated strains of lactobacillus plantarum dsm mcc1 23881 and lactobacillus gasseri mcc2 dsm 23882 microorganisms, and use thereof // 2603059
FIELD: biotechnology.SUBSTANCE: group of inventions refers to the strains of Lactobacillus plantarum DSM MCC1 23881 and Lactobacillus gasseri MCC2 DSM 23882 microorganisms and use thereof as antioxidant proteolytic ingredients for food product or food additive preparation. Strains generate conjugated linoleic acid (CLA), hydrogen peroxide (H2O2) and nitrogen monoxide (NO). Food products and food additives containing the above strains together or separately, are hypoallergenic, allergy and reduced irritation symptoms of lower urinary tract in males adult individuals, accompanying benign prostatic hyperplasia, and associated with oxidative stress and inflammation. Invention also discloses methods for production of food product or food additive.EFFECT: group of inventions relates to biotechnology and can be used in food industry.13 cl, 6 dwg, 13 tbl, 6 ex

Strain of bacteria escherichia coli-succinic acid producer (versions) and method for preparing succinic acid using this strain // 2603004
FIELD: biotechnology.SUBSTANCE: disclosed versions of the bacteria Escherichia coli, which are succinic acid producers. Escherichia coli bacterium is modified so that the genes ackA, pta, poxB, ldhA, adhE, ptsG are inactivated in it, expression of genes galP and glk is strengthened, and bacterium possessing activity of pyruvate carboxylase. Also disclosed a version of bacteria Escherichia coli, having inactivated genes ackA, pta, poxB, ldhA, adhE, ptsG, icIR reinforced by expression of genes galP and glk, and activity of pyruvate carboxylase. Also disclosed a version of bacteria Escherichia coli, having inactivated genes ackA, pta, poxB, ldhA, adhE, ptsG, enhanced by expression of genes galP and glk, enhanced by expression of genes aceE, aceF and lpdA, and activity of pyruvate carboxylase. Also disclosed a version of bacteria Escherichia coli, having inactivated genes ackA, pta, poxB, ldhA, adhE, ptsG, icIR, enhanced by expression of genes galP and glk, enhanced by expression of genes aceE, aceF and lpdA, and activity of pyruvate carboxylase. Also disclosed a version of bacteria Escherichia coli, having inactivated genes ackA, pta, poxB, IdhA, adhE, ptsG, pflB, enhanced by expression of genes galP and glk, enhanced by expression of genes aceE, aceF and lpdA, and activity of pyruvate carboxylase. Also disclosed a version of bacteria Escherichia coli, having inactivated genes ackA, pta, poxB, ldhA, adhE, ptsG, pflB, icIR, enhanced by expression of genes galP and glk, enhanced by expression of genes aceE, aceF and lpdA, and activity of pyruvate carboxylase. Disclosed a method for preparing succinic acid with using the above versions.EFFECT: group of inventions provides higher yield of succinic acid.19 cl, 2 tbl, 2 ex

Strains of streptococcus pneumoniae (versions) and method of producing protective protein fraction, possessing intraspecific immunogenic activity // 2601158
FIELD: medicine.SUBSTANCE: present group of inventions relates to immunology. Streptococcus pneumoniae strains are deposited in the State collection of pathogenic microorganisms of pathogenicity of III-IV groups of the Federal state budgetary institution “Scientific Center for Expertise of Means of Medical Application” of the Ministry of Health of Russia under registration numbers 296, 297, 298. These strains have intraspecific cross-protective activity and can be used to produce protein-containing fractions. Disclosed is a method for producing protein-containing fractions from the above strains to develop pneumococcal vaccines. For this purpose the strain is cultured, microbial cells are separated by microfiltration and inactivated by dimethyl ketone with subsequent water extraction and ultrafiltration to obtain a protein fraction with molecular weight of 30-100 kDa and cool dehumidification.EFFECT: obtaining effective preparations with intraspecific immunogenic activity with respect to the respective serotypes of S.pneumoniae, which can be used as a antipneumococcal vaccine of a wide spectrum of action.4 cl, 1 dwg, 4 tbl, 1 ex

Composition of bacterial strains, mixture for bioremediation and using said composition for removal of contaminants from soil, as well as method of soil cleaning from contaminants // 2601155
FIELD: biotechnology.SUBSTANCE: group of inventions can be used for soil cleaning from contaminants. Composition of 11 bacteria strains capable to metabolize aromatic nitro compounds and resistant to antibiotics and heavy metals is proposed. Composition is deposited in Collection of Industrial Microorganisms IAFB - of Institute of agricultural and food biotechnology in Warsaw No. KPP 2041p. Method of soil treatment using vaccine containing composition of strains is proposed.EFFECT: group of inventions provides effective bioremediation of soil, contaminated with aromatic nitro compounds, heavy metals and antibiotics.24 cl, 1 dwg, 12 tbl, 4 ex

Strain of candida albicans varietas stellatoidea for producing diagnostic allergen // 2601123
FIELD: medicine.SUBSTANCE: strain of Candida albicans var. stellatoidea deposited in the State collection of pathogenic microorganisms of III-IV pathogenicity groups of FSBI “Scientific Center for Expertise of Means of Medical Application” of the Ministry of Health of Russia under registration numbers 295 produces allergens having sufficient immunogenic properties.EFFECT: invention can be used for producing a diagnostic allergen.1 cl, 1 tbl, 1 ex

Preparation for cleaning soil and water from oil contaminants // 2600872
FIELD: biotechnology.SUBSTANCE: invention relates to microbiology and biotechnology, specifically to technology for production of preparations for cleaning soil and water from oil contaminants. Preparation contains a solid carrier substrate and immobilised on its surface a biomass of bacteria Pseudomonas panipatensis VKPM V-10593 in concentration of 1×109 cells/cm3. Solid carrier substrate used is expanded vermiculite.EFFECT: invention reduces duration of treatment of soil and water from oil contamination in wide temperature range (from +8 to 37 °C).1 cl, 7 tbl, 2 ex
Saccharomyces cerevisiae vkpm y-4066 yeast strain-ethyl alcohol producer // 2599435
FIELD: biotechnology.SUBSTANCE: invention relates to biotechnology. Saccharomyces cerevisiae A-1 strain with high yield, is deposited in the Russian National Collection of Industrial Microorganisms under registration number Saccharomyces cerevisiae VKPM Y-4066. Saccharomyces cerevisiae VKPM Y-4066 strain can be used in production of mixed fermentation cultured milk products and low-alcoholic drinks from milk whey, as well as ethyl alcohol.EFFECT: invention increases the output of ethyl alcohol.1 cl
Kluyveromyces lactis yeast strain - producer of ethyl alcohol // 2599434
FIELD: biotechnology.SUBSTANCE: invention relates to biotechnology. Strain of Kluyveromyces lactis A-2, having high efficiency, is deposited in Russian National Collection of Industrial Microorganisms under registration number Kluyveromyces lactis VKPM Y-4067. Kluyveromyces lactis VKPM Y-4067 strain can be used in production of mixed fermentation milk products and low-alcoholic drinks from milk whey, as well as in production of ethyl alcohol.EFFECT: invention increases the output of ethyl alcohol.1 cl
Strain of lactic bacteria enterococcus thailandicus - producer of lactic acid and antibiotic substances // 2599433
FIELD: biotechnology.SUBSTANCE: invention relates to biotechnology. Disclosed is an Enterococcus thailandicus K25 XIV (I) strain, having high antagonistic activity on opportunistic and pathogenic microflora. Strain is deposited in VKPM under registration number VKPM V-11832. Enterococcus thailandicus VKPM V-11832 strain can be used in food industry, as well as a base for producing a probiotic starter.EFFECT: invention reduces duration of fermentation of milk.1 cl
Strain of lactic bacteria enterococcus hirae - producer of lactic acid and antibiotic substances // 2599432
FIELD: biotechnology.SUBSTANCE: invention relates to biotechnology. Disclosed is an Enterococcus hirae tur R37 VX strain, having high antagonistic capacity with respect to opportunistic and pathogenic microflora. Strain is deposited in VKPM under registration number VKPM V-11829. Enterococcus hirae VKPM V-11829 strain can be used in food industry, as well as a base for producing a probiotic starter.EFFECT: invention reduces duration of fermentation of milk.1 cl
Lactic bacteria enterococcus hirae vkpm v-11835 strain - lactic acid and antibiotic substances producer // 2599431
FIELD: biotechnology.SUBSTANCE: invention relates to biotechnology. Strain Enterococcus hirae MC 25 37 (1) 10, having high biological activity and high rate of milk coagulation, is deposited in the Russian National Collection of Industrial Microorganisms under registration number Enterococcus hirae VKPM V-11835. Enterococcus hirae VKPM V-11835 strain can be used in food industry.EFFECT: invention allows to reduce time of milk coagulation.1 cl
Strain of lactic bacteria enterococcus mundtii - producer of lactic acid and antibiotic substances // 2599430
FIELD: biotechnology.SUBSTANCE: invention relates to biotechnology. Disclosed is an Enterococcus mundtii tur S37III strain, having high antagonistic capacity with respect to opportunistic and pathogenic microflora. Strain is deposited in VKPM under registration number VKPM V-11828. Enterococcus mundtii VKPM V-11828 strain can be used in food industry, as well as a base for producing a probiotic starter.EFFECT: invention reduces duration of fermentation of milk.1 cl
Strain of lactic bacteria enterococcus hirae - producer of lactic acid and antibiotic substances // 2599429
FIELD: biotechnology.SUBSTANCE: invention relates to biotechnology. Disclosed is an Enterococcus hirae M25b No. 23 strain, having high antagonistic capacity with respect to opportunistic and pathogenic microflora. Strain is deposited in VKPM under registration number VKPM V-11834. Enterococcus hirae VKPM V-11834 strain can be used in food industry, as well as a base for producing a probiotic starter.EFFECT: invention reduces duration of fermentation of milk.1 cl
Strain of lactic bacteria enterococcus durans - producer of lactic acid and antibiotic substances // 2599428
FIELD: biotechnology.SUBSTANCE: invention relates to biotechnology. Disclosed is an Enterococcus durans K37 VIII (1) strain, having high antagonistic capacity with respect to opportunistic and pathogenic microflora. Strain is deposited in VKPM under registration number VKPM V-11827. Enterococcus durans VKPM V-11827 strain can be used in food industry, as well as a base for producing a probiotic starter.EFFECT: invention reduces duration of fermentation of milk.1 cl
Strain of lactic bacteria enterococcus thailandicus - producer of lactic acid and antibiotic substances // 2599427
FIELD: biotechnology.SUBSTANCE: invention relates to biotechnology. Disclosed is an Enterococcus thailandicus M25a No. 15 strain, having high antagonistic activity on opportunistic and pathogenic microflora. Strain is deposited in VKPM under registration number VKPM V-11833. Enterococcus thailandicus VKPM V-11833 strain can be used in food industry, as well as a base of a probiotic starter.EFFECT: invention reduces duration of fermentation of milk.1 cl
Strain of lactic bacteria enterococcus thailandicus - producer of lactic acid and antibiotic substances // 2599426
FIELD: biotechnology.SUBSTANCE: invention relates to biotechnology. Disclosed is an Enterococcus thailandicus tur K 45 III strain, having high antagonistic capacity with respect to opportunistic and pathogenic microflora. Strain is deposited in VKPM under registration number VKPM V-11826. Enterococcus thailandicus VKPM V-11826 strain can be used in food industry, as well as a base for producing a probiotic starter.EFFECT: invention reduces duration of fermentation of milk.1 cl
Strain of lactic bacteria enterococcus hirae - producer of lactic acid and antibiotic substances // 2599425
FIELD: biotechnology.SUBSTANCE: invention relates to biotechnology. Disclosed is an Enterococcus hirae M37a No. 13 strain, having high antagonistic capacity with respect to opportunistic and pathogenic microflora. Strain is deposited in VKPM under registration number VKPM V-11831. Enterococcus hirae VKPM V-11831 strain can be used in food industry, as well as a base for producing a probiotic starter.EFFECT: invention reduces duration of fermentation of milk.1 cl
Strain of lactic bacteria enterococcus faecalis - producer of lactic acid and antibiotic substances // 2599424
FIELD: biotechnology.SUBSTANCE: invention relates to biotechnology and can be used in food industry. Disclosed is an Enterococcus faecalis M-37A No. 5 strain, having high antagonistic capacity with respect to opportunistic and pathogenic microflora. Strain is deposited in VKPM under registration number VKPM V-11830. Enterococcus faecalis VKPM V-11830 strain can be used in food industry and can be used as a base for producing a probiotic starter.EFFECT: invention reduces duration of fermentation of milk.1 cl

acromolecular complex of bacterial origin and use thereof for preventing or treating rheumatoid arthritis // 2599422
FIELD: medicine.SUBSTANCE: proposed is a bacterial macromolecular complex for preventing or treating inflammatory rheumatic disease and osteoarthritis. Complex is produced by CNCM I-3994 strain of Bifidobacterium longum bacteria. Complex consists of chains combining lipoprotein and oligosaccharide. Herewith the lipoprotein has the molecular weight ranging from 30kDa to 60 kDa, and the oligosaccharide is less than 15kDa and preferably less than 10kDa. Lipoprotein component consisting of all lipoproteins of every chain is 75-99 %, preferably 80-98 %, more preferably from 85 to 95% by weight of the total weight of the complex, and oligosaccharide component consisting of all oligosaccharides combined with each of the chains ranges from 1 to 25 %, preferably from 2 to 20 % and more preferably from 5 to 15 % of total weight of the complex. Amino acid sequence of the lipoprotein is SEQ ID 2. Saccharides forming the oligosaccharide component of the complex are selected from galactose (Gal), N-acetylgalactosamine (Gal Nac), glucose (Glc), N-acetylglucosamine (Glc Nac), rhamnose (Rham) and mannose (Man) and mixtures thereof. Average mass fraction of Gal is 5-20 mcg/mg of the complex, Man is 1-10 mcg/mg of the complex, Glc is 10-50 mcg/mg of the complex, Gal Nac is 2-10 mcg/mg of the complex, Glc Nac is 1-5 mcg/mg of the complex, Rham is 1-5 mcg/mg of the complex. Lipids comprising the lipoprotein component of the complex are selected from a group consisting of saturated fatty acids with the length of C14:0, C16:0 and C18:0 and their mixtures.EFFECT: invention also discloses pharmaceutical, food and nutritional compositions based on the said complex.12 cl, 9 dwg, 5 tbl, 9 ex

Bacillus amyloliquefaciens subsp.plantarum bs89 strain as agent for increasing productivity of plants and protection thereof from diseases // 2599416
FIELD: biotechnology.SUBSTANCE: invention relates to biotechnology and agriculture. Strain Bacillus amyloliquefaciens subsp. plantarum BS89, having high fungicidal activity against phytopathogenic fungi and bactericidal activity against phytopathogenic bacteria, is deposited in Departmental collection FGBNU VNIISKHM No. RCAM03458. Strain Bacillus amyloliquefaciens subsp. plantarum RCAM03458 can be used in agriculture as an effective agent for protecting plants from plant pathogenic fungi and bacteria, improving nutrition of supply of crops and increasing productivity of plants. At that for effective usage of Strain Bacillus amyloliquefaciens subsp. plantarum BS89 in composition of different biologies titre of bacterium must be 104--109Vc/mL.EFFECT: invention increases crop yield.1 cl, 16 tbl

ethod for growth inhibition of methicillin-resistant staphylococcus aureus strain // 2598272
FIELD: medicine.SUBSTANCE: invention refers to experimental medicine and can be used for growth inhibition of methicillin-resistant Staphylococcus aureus strain. Method provides for radiation treatment of bacterial cell suspension of methicillin-resistant Staphylococcus aureus strain with ruby light (λ -660 nm). Power density is 100 mW/cm2, radiation time is 10, 15 or 30 minutes in a continuous mode. Radiant exposure is 60, 90 and 180 J/cm2 respectively.EFFECT: invention simplifies the method for staphylococci growth inhibition.1 cl, 1 tbl
New lactic acid bacteria and method for production of silage or fermented fodder with its application // 2598270
FIELD: biotechnology.SUBSTANCE: proposed are lactic acid bacteria strain Lactococcus lactis SBS-0001 (NITE BP-1107) and lactic acid bacteria strain Lactococcus lactis SBS-0002 (NITE BP-1108) for silage production. Said strains are able to suppress amylic fermentation in raw material for silage or a fermented fodder with low sugar content and without sufficient quality of fermentation at using standard lactic acid bacteria. Also proposed is a microbial additive for silage production, the preparation of which contains one of the specified strains and a strain of lactic acid bacteria Lactobacillus paracasei SBS-0003 (NITE BP-1109) having acid resistance. Said microbial additive is used in the method of producing silage involving addition of the microbial additive to raw material and fermentation of the raw material containing the microbial additive for silage production at the temperature from 5 to 30 °C during 1 week and more.EFFECT: group of inventions allows to improve quality of silage fermentation.4 cl, 15 tbl, 2 ex

Lecanicillium muscarium fungus strain having insecto-acaricidal and antibiotic activity for fighting against sucking pests, fungal and bacterial diseases // 2598251
FIELD: agriculture.SUBSTANCE: invention relates to agricultural microbiology, in particular to protection of plants from pests and diseases. Lecanicillium muscarium MP-4 strain has insecto-acaricidal, as well as antibiotic activity to a complex of plant pathogenic fungi and bacteria. Strain is deposited in the State Collection of Microorganisms pathogenic for plants and their pests under the registration number of Lecanicillium muscarium G-033 VIZR and can be used for producing a preparation against a complex of sucking pests, mushroom and plant pathogenic fungi and bacteria.EFFECT: invention allows accelerating and increasing the death rate of sucking pests and mites and reducing the morbidity of plants caused by phytopathogenic bacteria and fungi.1 cl, 8 tbl, 9 ex

Strain paraphoma sp. producer of feosferid a // 2596928
FIELD: biotechnology.SUBSTANCE: invention relates to biotechnology, particularly, to production of biologically active substances and can be used for producing of feosferid A. Stain of mushroom Paraphoma sp., deposited in FGBNU VIZR at registration number VIZR 1.46, is proposed as a producer of a feosferid A.EFFECT: invention allows to raise output of feosferid A.1 cl, 2 ex
 
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