Indexing scheme associated with subclasses c12c-c12q and or c12s, relating to micro-organisms (C12R)

C   Chemistry; metallurgy(312744)
C12R              Indexing scheme associated with subclasses c12c-c12q; or c12s, relating to micro-organisms(2724)

Optimized recombinant protein encoding gene - human interferon beta analog // 2614264
FIELD: biotechnology.SUBSTANCE: nucleotide sequence encoding a recombinant protein - interferon beta analog, optimized for expression in E. coli cells. E. coli BL-30-B cell, intended for interferon beta recombinant protein production is obtained by transformation of E. coli BL (DE3) cells by rET30a plasmid containing the said nucleic acid. E. coli BL-32-B cell, intended for interferon beta recombinant protein production is obtained by transformation of E. coli BL (DE3) cells by rET32a plasmid containing the said nucleic acid.EFFECT: invention provides a recombinant protein.3 cl, 5 dwg, 9 tbl, 9 ex
Strain of bacteria micrococcus luteus 805 - producer of site-specific methyl-dependedn endonuclease mluvi // 2614262
FIELD: biotechnology.SUBSTANCE: strain Micrococcus luteus, deposited under registration number B-12410 in The All-Russia Collection of Industrial Microorganisms of GosNIIGenetika FSUE is the producer of the site-specific methyl-dependent endonuclease MluVI. The invention provides a new MD-endonuclease enzyme which recognizes and cleaves both strands of the DNA sequence 5'-GCGC^NGCGC-3'/3'-CGCGN^CGCG-5' before the central nucleotide (N) with formation of single-nucleotide 5'-protruding ends in the presence in a given sequence of at least six C5-methylized cytosines and can be used for large-site-specific hydrolysis of DNA containing C5-methyl-cytosine bases.EFFECT: increase the efficiency of strain application.2 dwg, 1 tbl, 3 ex
ethod of detecting of cell culture contamination with viruses by immunoperoxidase method // 2614256
FIELD: biochemistry.SUBSTANCE: invention relates to biochemistry. Method of detecting contamination with viruses of cell culture by immunoperoxidase method is described, involving reaction of antigen with labelled antibodies in clean cell culture and accounting of reaction results by color density of formed complex under microscope. Wherein reaction uses tablets with analyzed cell culture, contaminated with diarrhoea virus, and after adding into tablet wells of 0.10–0.12 ml of specific homologous serum diluted by 1:64 – 1:128 it is incubated, washed, then 0.10–0.12 ml of anti-specific immunoenzymometric conjugate is introduced, consisting of peroxidase marked antibodies against globulins of cattle blood serum, it is incubated, washed, substrate mixture is introduced consisting of 5-aminosalicylic acid and hydrogen peroxide, and 30–40 minutes later results are accounted under light microscope by formation of brown color in virus-containing cells. Invention improves control system of cell lines latent contamination with viruses used in laboratory practice and biological industry.EFFECT: invention allows to prevent biological contamination and is extremely important stage in production of vaccines and diagnostic preparations; this method can be used for certification at absence of viruses in again coming cell cultures; invention is practically feasible, cheap method of monitoring of cell cultures for content of virus and may be recommended for extensive use both in scientific research and practical virological laboratories, and in production in making diagnostic preparations and vaccines.1 cl, 2 ex

icroorganisms producing o-acetyl homoserine and method for o-acetyl homoserine production using this microorganism // 2614253
FIELD: biotechnology.SUBSTANCE: this microorganism is characterized by weakened or inactivated endogenous citrate synthase activity. As a result of this modification, the is microorganism capable of producing O-acetyl homoserine with high yield. The invention also relates to a method for O-acetyl homoserine production. This method comprises culturing of the said microorganism and separation of O-acetyl homoserine produced by culturing of this microorganism.EFFECT: invention allows to obtain O-acetyl homoserine with high yield.7 cl, 2 dwg, 10 tbl, 4 ex
Yeast transformant schizosaccharomyces pombe, producing lactic acid (versions) method for production thereof (versions), method for lactic acid microbiological synthesis using this transformant // 2614233
FIELD: biotechnology.SUBSTANCE: transformant with gene ldh, encoding lactate dehydrogenase from Lactobacillus acidophilus or an enzyme with amino acid sequence homologous to it by at least 93% are proposed. Also a transformant with gene ldh, encoding lactate dehydrogenase from Lactobacillus acidophilus or an enzyme with amino acid sequence homologous to it by at least 93% are proposed, wherein one or several genes encoding enzymes involved in the ethanol biosynthesis pathway are deleted or inactivated. A transformant with gene ldh, encoding lactate dehydrogenase from Lactobacillus acidophilus or an enzyme with amino acid sequence homologous to it by at least 93% are proposed together with gene ldh encoding lactate dehydrogenase from Lactobacillus plantarum or Lactobacillus pentosus. A transformant with gene ldh, encoding lactate dehydrogenase from Lactobacillus acidophilus or an enzyme with amino acid sequence homologous to it by at least 93% are proposed together with gene ldh encoding lactate dehydrogenase from Lactobacillus plantarum or Lactobacillus pentosus,wherein one or several genes encoding enzymes involved in the ethanol biosynthesis pathway are deleted or inactivated. Also, versions of the preparation method for these transformants and the method for microbial synthesis of lactic acid are proposed.EFFECT: efficient production of lactic acid at low concentrations of by-product.13 cl, 10 dwg, 8 ex

Fungi strain from sordariomycetes class - producer of eremoxylarin a antibiotic // 2614126
FIELD: biotechnology.SUBSTANCE: ascomycetous fungi strain of Sordariomycetes INA 01108 class has the ability to produce eremoxylarin A antibiotic. Ascomycetous fungi strain of Sordariomycetes class is deposited in the Russian National Collection of Microorganisms of the Federal State Budget Institution Science Institute of Biochemistry and Physiology of Microorganisms named after G.K. Skryabin, RAS under accession number VKM F-4676D and can be used in medicine.EFFECT: invention allows to increase the yield of eremoxylarin A antibiotic.4 dwg, 2 tbl, 1 ex
Optimized gene encoding recombinant ipfiii protein // 2614124
FIELD: biotechnology.SUBSTANCE: nucleotide sequence encoding a recombinant interferon lambda protein is optimized for expression in E. coli cells and cloned into the rET30a plasmid. E. coli BL-30-L cell, intended for interferon lambda recombinant protein production is obtained by transformation of E. coli BL (DE3) cells by rET30a plasmid containing the said nucleic acid. The invention provides a recombinant interferon lambda protein with biological activity of 2.1*109 U/mcmol in MDBK/VSV system.EFFECT: increased biological activity.2 cl, 4 dwg, 3 tbl, 6 ex
Bifidobacterium adolescentis 150 strains and bifidobacterium angulatum gt 102, synthesizing gamma-aminobutyric acid // 2614110
FIELD: biotechnology.SUBSTANCE: invention group relates to B.adolescentis 150 B.angulatum GT 102 bacteria strains. B.adolescentis B.angulatum strains are deposited in the Russian National Collection of Industrial Microorganisms under accession numbers VKPM Ac-1974 and VKPM Ac-1973 and have the ability to synthesise gamma-aminobutyric acid (GABA). These strains can be used to obtain probiotic preparations.EFFECT: invention allows to expand the range of gamma-aminobutyric acid producers.2 dwg, 5 tbl, 6 ex
Plankton strain chlorella kessleri, intended for production of biomass // 2613424
FIELD: biotechnology.SUBSTANCE: strain is deposited in the Russian National Collection of Industrial Microorganisms under accession number All Russian Collection of Industrial Microorganisms Al-12 and can be used for production of biomass used for feeding of livestock animals, as well as the algolizant of water bodies in course of their biological rehabilitation and wastewater treatment.EFFECT: plankton strain of unicellular green alga Chlorella kessleri NF has a high productivity.1 dwg, 1 tbl, 1 ex
ethod of culture diagnosis of tuberculosis infection // 2613366
FIELD: biotechnology.SUBSTANCE: invention relates to biotechnology. Method involves following. Collected sputum is subjected to fast freezing at temperature from -20 to -25 °C without preserving agents and kept at this temperature from 16 to 24 hours. It is unfrozen at room temperature and treated with 4 % solution of sodium hydroxide containing N-acetyl-L-cysteine during 20-25 minutes. Sodium phosphate buffer 1 M is added and mixture is centrifuged at 3,000–3,100 g for 20–25 minutes. Sample is decanted to separate precipitate. Iron oxide is added to precipitate in final concentration of 0.04 M, while stirring, in sodium phosphate buffer 1 M, pH 6.8 and inoculated on nutrient medium Lowenstein-Jensen.EFFECT: invention allows reducing time for obtaining positive results of dense nutrient medium.1 cl

New bacteria and their extracts, and use thereof in dermatology // 2612790
FIELD: biotechnology.SUBSTANCE: group of inventions relates to biotechnology. Non-pathogenic strain of gram-negative bacteria is proposed, which is deposited in CNCM April 8, 2010 under number I-4290. Said strain is intended for use in treating or preventing skin inflammatory diseases. Bacterial extract is disclosed, which is obtained by culturing and lysis of above strain of bacteria. Bacterial extract has protein profile, which as shown by denaturation electrophoresis in polyacrylamide gel, includes 12 detectable strips, including three main strips corresponding to molecular weights in range between 30 kDa and 36 kDa, 41 kDa and 45 kDa, and 47 kDa and 51 kDa, respectively. Composition for treating skin inflammatory diseases is disclosed containing above strain and/or bacterial extract as active ingredient in effective amount and one or more dermatologically compatible fillers.EFFECT: group of inventions provides induction of cytokine IL-10, IL-12 and TNFα, inhibition of cytokine IL-4 and IL-6, activation of Langerhans cells, normalization of Th1/Th2 balance, reduction of expression of IgE, induction of formation of antimicrobial peptides and proteins hBD-2, hBD-3, S1007A, LL-31, PI3, RNase7 and NOD2.8 cl, 12 dwg, 9 tbl, 5 ex

New recombinant strain of filamentous fungi penicillium canescens cs15, producing cellulase clostridium thermocellum and method of its cultivation // 2612158
FIELD: biotechnology.SUBSTANCE: invention relates to a recombinant strain of filamentous fungi Penicillium canescens CS15 VKM F-4679D, secreting thermostable cellulase Cel48S from Clostridium thermocellum. The invention also discloses a method for culturing of the said strain in a particular nutrient medium. The said nutrient medium consists of 35 g/l of soybean hulls, 40 g/l of corn extract, 6 g/l of KH2PO4, 0.23 g/l of CaCl2, 0.3 g/l of MgSO4* 7H2O, 10 g/l of yeast extract and 10 g/l of wheat bran.EFFECT: invention allows efficient production of cellulase from Clostridium thermocellum in a heterologous host Penicillium canescens.2 cl, 6 dwg, 1 tbl, 4 ex
Penicillium vulpinum micromycetes strain f-1523 with antibacterial activity on anthrax agent bacillus anthracis // 2612150
FIELD: medicine.SUBSTANCE: invention relates to medical microbiology and biotechnology. Penicillium vulpinum micromycetes strain, having antibacterial activity on anthrax agent Bacillus anthracis, is deposited in State collection of pathogenic microorganisms and cell cultures (GKPM-Obolensk) under registration number F-1523. Fugate of cultural fluid of micromycetes strain Penicillium vulpinum F-1523 in dose of 100 mcl shows maximum activity in relation to Bacillus anthracis STI-1 on second day of cultivation.EFFECT: invention provides use of Penicillium vulpinum F-1523 in suppressing development of anthrax agent.1 cl, 3 tbl, 3 ex

Competitive and effective strains of bacteria // 2611199
FIELD: agriculture.SUBSTANCE: composition of inoculum for enhancing plant growth is disclosed, which is suitable for seeds covering. Above composition contains agronomically acceptable carrier and at least one strain of Bradyrhizobia japonicum. Wherein at least one strain of Bradyrhizobia japonicum is selected from group, consisting of strain Bradyrhizobia japonicum NRRL B-50592, strain Bradyrhizobia japonicum NRRL B-5059, strain Bradyrhizobia japonicum NRRL B-50586, strain Bradyrhizobia japonicum NRRL B-50588, strain Bradyrhizobia japonicum NRRL B-50587, strain Bradyrhizobia japonicum NRRL B-50589, strain Bradyrhizobia japonicum NRRL B-50591, strain Bradyrhizobia japonicum NRRL B-50590, strain Bradyrhizobia japonicum NRRL B-50594, strain Bradyrhizobia japonicum NRRL B-50726, strain Bradyrhizobia japonicum NRRL B-50727, strain Bradyrhizobia japonicum NRRL B-50728, strain Bradyrhizobia japonicum NRRL B-50729 and/or strain Bradyrhizobia japonicum NRRL B-50730. Covered bean plant seed is also disclosed, wherein cover contains composition of inoculum. Said seed is intended for production of bean plant containing composition of inoculum and having enhanced growth.EFFECT: group of inventions increases efficiency of stimulation of growth of bean plants.60 cl, 6 dwg, 7 tbl, 5 ex

ethod for extraction of lipids from biomass of microalgae chlorella and yeast yarrowia lipolytica // 2610675
FIELD: chemistry.SUBSTANCE: invention refers to biotechnology. A method is claimed for extraction of lipids from microalgae of Chlorella genus and yeast Yarrowia lipolytica for producing biodiesel fuel. The method includes supplementation of the culturing stage of microalgae Chlorella with culturing stage of yeast Yarrowia lipolytica W23. Lipid fraction is extracted from the microalgae and yeast biomass using organic solvents chloroform - methanol in ratio 2:1-1:2, the residual microalgae biomass and residual yeast biomass being hydrolyzed at a temperature of 80-140°C for 20-90 minutes, and this biomass in the form of aqueous suspension is used as a nutrient medium for culturing the yeast Yarrowia lipolytica W23.EFFECT: invention provides for high lipid output: up to 37% based on dry weight.6 cl, 1 dwg, 1 tbl, 6 ex

ethod of producing recombinant anti-tumor toxin based on protein barnase-barstar and targeted polypeptide darpin with effect of instant cancellation of cytotoxic action // 2610179
FIELD: biochemistry.SUBSTANCE: invention relates to biochemistry, biotechnology and medicine, namely to method of producing recombinant anti-tumor toxin-chimeric bifunctional protein, consisting of targeted polypeptide Darpin 9-29, specific to histochemical marker HER2/neu, and highly active bacterial ribonuclease barnase, connected with flexible peptide linker. For implementation of this method recombinant strain is produced Escherichia coli BL21(DE3)/pETDa by embedding into strain of E.coli BL21(DE3) plasmid pETDa. Said plasmid is produced by cloning of recombinant DNA fragment, coding said chimeric protein, in plasmid vector pET22 at restriction sites NdeI/HindIII.EFFECT: present invention enables to obtain targeted protein characterized by low risk of side effects due to instant cancellation of cytotoxicity for targeting on HER2/neu therapy of tumors overexpressing HER2 receptor.3 cl, 8 dwg, 3 tbl, 11 ex
icrobial composition for processing of organic wastes of human life, animal husbandry and poultry farming // 2609654
FIELD: biotechnology.SUBSTANCE: invention relates to biotechnology and microbiology, namely to biological preparations and microbial compositions for degradation of organic wastes, and can be used for fast, efficient processing of organic wastes of human life, animal breeding and poultry farming in high-quality organic fertilizer. Microbial composition contains Delftia lacustris BMCH-IB-C 142 BKM B-3032D, Cellulomonas persica BMCH-IB-C 35 BKM Ac-2727D, Rhodococcus qingshengii BMCH-IB-ONF 10 BKM Ac-2728D, Rhizobium nepotum BMCH-IB-ONF 5 BKM B-3028D, Bacillus subtilis BMCH-IB-ONF 14 BKM B-3029D, Paenibacillus endophyticus BMCH-IB-ONF 7 BKM B-3030D, Lactobacillus sp. BMCH-IB-M 3 BKM B-3031D, Bifidobacterium bifidum BMCH-IB-M 4 BKM Ac-2729D in given ratio.EFFECT: invention shortens duration of processing of organic wastes.1 cl, 2 tbl, 7 ex
Lactobacillus acidophilus bacteria strain used to obtain fermented milk products // 2609653
FIELD: biotechnology.SUBSTANCE: invention relates to biotechnology and can be used in dairy industry, medicine and veterinary science. Presented LIA-T-193 strain of Lactobacillus acidophilus bacteria possesses high acid-forming activity. Lactobacillus acidophilus strain is deposited in the ARRIAM under the registration number of RCAM01438. Strain can be used in production of “Ulcerine” acidophilic starter, also for preparation of acidophilous milk and cultured milk product. Can be used for feeding farm animals and poultry.EFFECT: invention enables to obtain cultured milk products with high antagonistic activity to undesirable microflora.1 cl, 3 ex

Lactobacillus paracasei 1 bacterial strain used for probiotic preparation production // 2608871
FIELD: biotechnology.SUBSTANCE: invention relates to microbiology and biotechnology. Strain Lactobacillus paracasei 1 has high antagonistic activity, high level of acid formation, improved adhesive properties, resistance to some antibiotic preparations and high rate of biomass accumulation. Strain Lactobacillus paracasei is deposited in Russian National Collection of Industrial Microorganisms under registration number VKPM V-11821 and can be used in production of probiotics.EFFECT: invention allows to normalize piglets intestinal microflora and enhance livestock livability.1 cl, 1 dwg, 7 tbl, 6 ex
Biological product for stimulating the growth of plants and their protection against phytopathogens on the basis of strains of trichoderma, strains of trichoderma for the production thereof (variants), method for producing a biological product on the basis of such strains // 2607785
FIELD: biotechnology.SUBSTANCE: group of inventions relates to biotechnology and can be used to create bioprotection of plants against phytopathogens and to stimulate their growth. Group of inventions comprises the strains of the fungus of Trichoderma longibrachiatum species (3 options); biological product for stimulating the growth of plants and their protection against phytopathogens on the basis of these strains and the method for producing a biological product. Strains of the Trichoderma longibrachiatum fungus are deposited at the ARRIAM FSBSI under registration numbers: RCAM 03324, RCAM 03323, RCAM 03325 respectively. Biological product contains spores and fragments of mycelium of above strains from 2×109 to 4×109 CFU/cm3; has antagonistic activity against phytopathogenic fungi of the Fusarium and Cladosporium species. Method of producing a biological product involves a two-stage joint cultivation of strains in a quantitative ratio of 1:1:1 consistently on two nutrient media for 4–5 days. This group of inventions provides increase in seed germination on 4–15 % and the increase of linear sizes of plants on 0.5–24 %.EFFECT: increased seed germination and the increase of linear sizes of plants.5 cl, 2 tbl, 5 ex

Strain of lactic acid bacteria, drug, food product, beverage, as well as fodder containing strain of lactic acid bacteria // 2607370
FIELD: biotechnology.SUBSTANCE: group of inventions relates to biotechnology. Strain Lactobacillus paracasei MCC1849 with high stimulating production of IL-12 activity.EFFECT: strain is used as ingredient of medicinal agent for immune stimulation, agent against influenza virus, food product, beverage, fodder and agent stimulating production of IL-12.8 cl, 2 dwg, 3 tbl, 3 ex
Strain of microorganisms achromobacter spanius 10-50-ts2 as agent improving plant resistance to chloride salinity // 2607028
FIELD: biotechnology.SUBSTANCE: invention relates to agricultural biotechnology. Invention represents strain of microorganisms Achromobacter spanius 10-50-TS2, deposited in Russian national collection of industrial microorganisms FGUPGosNIIGenetika under No. B-12405, as agent for increasing resistance of plants in conditions of chloride salinity of soils. Strain of bacteria Achromobacter spanius 10-50-TS2 No. B-12405 increases germination power of seeds by 40 % (p < 0.001), laboratory capacity by 35.2 % (p < 0.001), increases length of germs by 59 %, weight of seedlings in 2 times, increases number of roots by 13.3 %, root mass in 2 times under conditions of chloride salinity.EFFECT: higher resistance of plants.1 cl, 1 tbl
Biotransformation phenylmethyl sulphide in (r)-sulphoxide using immobilized cells gordonia terrae iegm 136 // 2607027
FIELD: biotechnology.SUBSTANCE: invention relates to biotechnology, in particular to production of (R)-phenylmethylsulfoxide. Method involves oxidation of phenylmethylsulfoxide using biocatalyst-immobilized in cryogel of polyvinyl alcohol of cells Gordonia terrae IEGM 136. Process is carried out in mineral medium, containing n-hexadecane in amount not exceeding 0.1 vol. %.EFFECT: invention allows to increase output of the end product and optimize the process of its production.3 cl, 2 dwg, 3 tbl, 8 ex
Dry bacterial starter for production of fermented milk products and production method thereof // 2607023
FIELD: biotechnology.SUBSTANCE: declared group of inventions relates to biotechnology. Method of production of dry bacterial starter for production of fermented milk products involves separate cultivation of strains Streptococcus thermophilus KD7 41 №2 VKPM V-10403 and Streptococcus thermophilus ZL-047 VKPM V-10707. Fermentation of each strain is carried out to produce a liquid biomass. Carried out concentrating of the resulting liquid biomass of each strain, mixing of each strain with a protective medium at a ratio of 1:2–1:4 with further separate drying and mixing the obtained biomass Streptococcus thermophilus KD7 41 №2 VKPM V-10403 and Streptococcus thermophilus ZL-047 VKPM V-10707 in ratio of – 3:7 with producing dry bacterial starter.EFFECT: invention allows to extend storage life of starter in the high content of active living cells of starter and cut the duration of milk fermentation.4 cl, 1 ex
Test strain leptospira of interrogans serogroup icterohaemorrhagiae serovar copenhageni for detection of antibodies to l icterohaemorrhagiae // 2607006
FIELD: biotechnology; medicine.SUBSTANCE: invention relates to medical biotechnology and can be used for detection of antibodies to L. icterohaemorrhagiae. Test strain Leptospira interrogans of Icterohaemorrhagiae serogroup copenhageni serovar with evident antigenic and immunogenic properties is deposited in State collection of pathogenic microorganisms and cell cultures "GKPM-Obolensk" under registration number V-7745.EFFECT: invention provides serological diagnosis of leptospirosis, monitoring of distribution of icterohaemorrhaghic leptospirosis among people and among different species of animals.1 cl, 1 tbl, 2 ex

Lactobacillus mucosae strain for producing fermented food products // 2606770
FIELD: food industry.SUBSTANCE: disclosed are Lactobacillus mucosae for producing fermented food products and a food product containing said strain. Lactobacillus mucosae strain is deposited in CNCM under number I-4429. Strain has capability to reduce permeability of intestinal barrier.EFFECT: invention can be used to alleviate conditions, including dysfunction of intestinal barrier, in particular conditions related to increase in permeability of intestinal barrier.3 cl, 5 dwg, 3 tbl, 5 ex

ethod of producing capsular polysaccharide with pneumococcal serotype // 2606022
FIELD: biotechnology.SUBSTANCE: present invention relates to biotechnology. Method of producing capsular polysaccharide with pneumococcal serotype is disclosed. Bacterial cells are cultured at culture fluid pH in range of 7.0 to 9.4. Then cultivation is stopped at the moment between culture fluid absorption coefficient is constant and absorption coefficient begins to fall. Additional cultivation is performed without pH regulation until culture fluid pH reaches value of 5.5 or less. Then lysing agent is added to obtained culture fluid for cell lysis. Precipitated proteins and cellular debris are removed to obtain purified cell lysate. Capsular polysaccharide is separated and purified from obtained lysate.EFFECT: invention eliminates process of depositing protein by increasing acidity by means of pH regulator, which simplifies process and does not lead to modification of capsular polysaccharide and formation of hazardous substances.13 cl, 6 dwg, 10 tbl, 3 ex
Recombinant plasmid dna pefn877, determining expression of the hybrid polypeptide with properties of 10-th fibronectin domain on the surface of escherichia coli cells, and strain of bacteria escherichia coli bl21(de3)plyss/pefn877 - producer of hybrid polypeptide with properties of 10-th fibronectin domain on the cells surface // 2606014
FIELD: biotechnology.SUBSTANCE: invention relates to biotechnology and can be used for producing the hybrid polypeptide alternative carcass protein 10-th human fibronectin domain. Recombinant plasmid DNA pEFN877 designed on the basis of plasmid pET20b(+) length 3.654 t.p.o. Obtained plasmid pEFN877 comprises hybrid of gene EFN877 with SEQ ID NO: 8, coding amino acid sequences of esterase P. cryohalolentis K5T, 10-th domain of human fibronectin and autotransporter P. cryohalolentis K5T in a single reading frame, arranged in accordance with fig. 1. Bacterial strain E. coli BL21(DE3)pLysS/pEFN877 is obtained by transformation of competent cells E. coli BL21(DE3)pLysS of plasmid DNA pEFN877.EFFECT: invention allows to obtain cell strain E.coli – producer of hybrid polypeptide with properties of 10-th human fibronectin domain on the cells surface.2 cl, 4 dwg, 9 ex

ethod for stimulating growth processes of chlorella microalgae // 2605636
FIELD: biotechnology.SUBSTANCE: invention relates to biotechnology and can be used for improving productivity of cultivation of microalgae Chlorella. Method comprises treating of microalgae Chlorella vulgaris IFR № C-111 by ozone-air mixture with ozone concentration of not more than 7.2 mg/m3 during 6 minutes a daylight on the first and second days after sowing.EFFECT: invention allows to increase output of biomass of microalgae Chlorella vulgaris IFR № C-111.1 cl, 4 dwg, 4 tbl

ethod for producing allergen for differentiating dried purified tuberculine nonspecific reactions for mammals // 2605633
FIELD: veterinary science.SUBSTANCE: invention relates to veterinary microbiology. Method for producing allergen for differentiating dried purified tuberculine nonspecific reactions for mammals is proposed. Method comprises growing Mycobacteria m.Avium strain 2282 (3rd Ranion group), M.scrofulaceum (2nd Ranion group) and M.fortuitum (4th Ranion group) separately on a liquid nutrient medium Soton at temperature of 37-38 °C for 4-8 weeks and their inactivation. Protein from a culture filtrate of each strain is separately deposited with trichloroacetic acid to the final concentration of 6-8 %, followed by dialysis of protein solution, filtration through membrane plates (Immersible CX-10), mixing protein solutions in equal portions by content of activity units (u), filtration, stabilisation and packaging.EFFECT: method provides higher purity of the preparation and higher quality of differential diagnosis of dried purified tuberculin nonspecific reactions for mammals.1 cl, 2 tbl

Recombinant strain of yeast pichia pastoris - producer of the secreted thermostable xyloglucanase coded by synthetic gene, and method for microbiological synthesis of secreted thermostable xyloglucanase based on this strain // 2605629
FIELD: biochemistry.SUBSTANCE: invention relates to biochemistry, genetic engineering and biotechnology, in particular, to strain Pichia pastoris VKPM Y-4269. This is a producer of secreted thermostable xyloglucanase of GH74 class. Said xyloglucanase encoded by synthetic gene corresponding to SEQ ID NO 1. Present invention also relates to a method for microbiological synthesis of xyloglucanase. Above method involves culturing a recombinant microorganisms, containing gene xyloglucanase, in aerobic conditions in a nutrient medium to maximum accumulation of the end product. This nutritional medium includes sources of carbon, nitrogen and mineral additives. Above method is characterised by that is as a producer is used a recombinant strain Pichia pastoris VKPM Y-4269.EFFECT: present invention enables to obtain secretable thermostable xyloglucanase of class GH74 with high output and purity.2 cl, 2 dwg, 3 ex

ethod for obtaining spore culture on basis of bacterial strain bacillus species 1839 // 2605543
FIELD: biotechnology.SUBSTANCE: invention relates to biotechnology, microbiology and can be used for production of spore material of bacteria strain Bacillus sp. 1,839. Method involves inoculation of nutrient medium with bacteria of strain Bacillus sp. 1,839 with further cultivation on it. Biomass is separated by centrifugation at 1,000-1,500 rpm. In obtained bacteria biomass hypertonic phosphate buffer is added, containing potassium chloride, sodium hydrogen phosphate, potassium dihydrogen phosphate and sodium chloride at given ratio. Bacillus sp. 1,839 incubation is performed until formation of spores. For removal of remained vegetative cells thermal treatment is performed by heating obtained precipitate of spores on water bath at least for 10 minutes at 70-90 °C, supernatant is centrifuged and removed.EFFECT: invention simplifies method and allows obtaining spore culture in any bacterial or veterinary laboratories.3 cl, 4 ex

Recombinant chimeric protein sumo3-apoa-i for producing mature human apolipoprotein a-i, pichia pastoris yeast strain - producer of recombinant chimeric protein sumo3-apoa-i and method of producing mature human apolipoprotein a-i // 2605326
FIELD: genetic engineering.SUBSTANCE: to increase yield of mature human apolipoprotein A-I it is produced by enzymatic hydrolysis of SUMO-specific protease SP2 of recombinant chimeric protein SUMO3-apoA-I, synthesised by Pichia pastoris yeast strain. Said strain is constructed by transformation of Pichia pastoris X-33 yeast strain with linearised plasmid DNA pPsecSUMO3-apoA-I. Recombinant chimeric protein SUMO3-apoA-I includes from N-terminal amino acid sequence of ubiquitin-like protein of human SUMO3, fused at C-terminal with amino acid sequence of mature human apoA-I protein.EFFECT: invention can be used for producing mature human apolipoprotein A-I.3 cl, 1 tbl, 5 dwg, 7 ex

Expression system and method of producing non-modified recombinant proteins in escherichia coli using it // 2604796
FIELD: biotechnology.SUBSTANCE: group of inventions relates to biotechnology. DNA construct is disclosed, which codes fused precursor protein, in which auxiliary amino acid sequence is bound to N-end of mature target polypeptide sequence with transition site intended for identification and splitting of hybrid precursor by specific protease to form non-modified protein of interest. Above site represents truncated at N-end by 23 amino acid residues SUMO protein of S. cerevisiae (Smt3), which includes site of splitting with SUMO-hydrolase (Ulp1). On basis of DNA construct coding version of precursor, which comprises glutathione-S-transferase (GST) as auxiliary sequence and negatively charged peptide and human interferon (IFN) α2b as target protein recombinant E. coli strain is obtained. Method of producing non-modified IFN α2b using above E. coli strain is also disclosed.EFFECT: group of inventions enables to obtain target protein with high purity and activity.7 cl, 9 dwg, 2 tbl, 5 ex

Culture medium for growing of bacteria // 2604789
FIELD: microbiology.SUBSTANCE: invention relates to microbiology. Nutrient medium for growth of bacteria is disclosed. Nutrient medium contains pancreatic casein overcook, meat pepsin overcook, heart pancreatic overcook, yeast extract, starch, water, violuric acid and infusion of beef.EFFECT: invention provides simultaneous growth of maximum possible number of bacteria in plated material.7 cl, 6 tbl

ethod of cleaning freshwater ecosystems from oil and oil products at high latitudes // 2604788
FIELD: biotechnology.SUBSTANCE: invention relates to biotechnology. Method of cleaning freshwater ecosystems from oil and oil products is proposed. Method comprises contacting oil hydrocarbons to be decomposed with a biopreparation, consisting of a mixture of oil-oxidising Microbacterium species VKM Ac-2614D, Pseudomonas migulae BKM B-2761D, Rhodococcus erythropolis VKM Ac-2612D, Rhodococcus erythropolis VKM Ac-2611D strains, taken in ratio of 1:1: 1:1. Cell titre in the ready preparation is, at least, 1010 cells per 1 g.EFFECT: method provides high rate of biodegradation of oil in aqueous media in temperature range from 0 to +25 °C, including ice conditions.5 cl, 4 dwg, 7 tbl, 6 ex

Strain of bacteria serratia marcescens m-10 is producer of extracellular non-specific endonuclease with marked antiviral action // 2604183
FIELD: biotechnology.SUBSTANCE: strain is deposited in VKM under number VKM B-2931D. Endonuclease activity of strain to 36 hours cultivation on meat-peptone infusion broth is 28,720 units/ml of cultural liquid.EFFECT: strain of bacteria Serratia marcescens, being a producer of nonspecific bacterial endonuclease.1 cl, 1 tbl, 1 ex

ethod of microalgae biomass flocculation // 2603733
FIELD: biotechnology.SUBSTANCE: invention relates to biotechnology and can be used in municipal (water treatment and wastewater) and agriculture, mining industry, medicine, biotechnological industry. Method of microalgae biomass flocculation from suspending medium is proposed. Method involves successive addition to suspending medium with biomass of microalgae coagulant six-water iron (III) chloride in concentration of 20-40 mg/l and flocculants: polyacrylamide and polyethylene oxide in amount, respectively, of 2-5 mg/l and 1-4 mg/l. Flocculation of biomass of microalgae is carried out, while stirring at temperature of 10-40 °C.EFFECT: invention provides increased rate of deposition of microalgae biomass.8 cl, 3 tbl

Strain escherichia coli bl21(de3)gold/petcypopti - producer of human recombinant cyclophilin a // 2603283
FIELD: biotechnology.SUBSTANCE: invention relates to biochemistry, genetic engineering and biotechnology, namely to strain Escherichia coli BL21(DE3)GoldpETCYPopti. This strain is producer of recombinant human cyclophilin A. Strain is produced by cell transformation of strain Escherichia coli BL21(DE3)Gold by pETCYPopti plasmid. Said plasmid has size of 5858 base pairs and contains fragment XhoI-NdeI of vector pET-22b(+) carrying gene of ampicillin resistance ampR, promoter and terminator of phage T7 RNA-polymerase and polylinker, wherein fragment, carrying gene of human recombinant cyclophilin A, is cloned at sites XhoI-NdeI with size of 498 base pairs, SEQ ID NO: 1.EFFECT: present invention enables to obtain human cyclophilin A with high output and purity.1 cl, 3 dwg, 1 ex
Bioinsecticide for controlling large bee moth // 2603271
FIELD: agriculture.SUBSTANCE: invention relates to agriculture, in particular, to bee keeping, and can be used for controlling large bee moth. Bioinsecticide for large bee moth contains a Bacillus thuringiensis ARRIAM RCAM 00045 strain and filler, for example, NaCl, in a given ratio of components.EFFECT: invention enables to recover bee families of large bee moth and preventive treatment of injuries of bee families of large bee moth larvae during entire honey harvesting season improves safety of bee families.1 cl, 2 ex

ethod for detoxification of white phosphorus using microorganism strain trichoderma asperellum vkpm f-1087 // 2603259
FIELD: biotechnology.SUBSTANCE: invention relates to biotechnology, specifically to a method for detoxification of white phosphorus in contaminated soil. Decontamination is performed by treatment of white phosphorus-contaminated soil with a strain of Trichoderma asperellum VKPM F-1087. Method enables detoxification of soil containing white phosphorus in concentration up to 1.0 wt%, exceeding the maximum allowable concentration 5,000 times.EFFECT: method enables detoxification of soil.1 cl, 1 dwg
Strain of bacteria desulfovibrio species vk-9 for purification of acidic wastewater from heavy metal ions // 2603249
FIELD: microbiology.SUBSTANCE: invention relates to industrial microbiology. Bacterial strain Desulfovibrio sp. VK-9 has high resistance to high concentrations of copper ions (up to 125 mg/l), nickel ions (up to 250 mg/l), cobalt ions (up to 350 mg/l) and cadmium ions (up to 60 mg/l) and can be used for purification of acidic wastewater from machine building, instrument-making, electrical, mining enterprises, electroplating industries, as well as for acidic mine water purification from high concentrations of copper ions and other heavy metals. Strain Desulfovibrio sp., is deposited in Russian National Collection of microorganisms of Institute of biochemistry and physiology of microorganisms RAS IBFM under registration number VKM V-3000D.EFFECT: invention allows to increase degree of wastewater purification from heavy metal ions.1 cl, 2 tbl, 4 ex

Strain of microalgae chlorella vulgaris beyerinck - producer of mixture of aromatic substances similar to resinoid of oakmoss // 2603099
FIELD: biotechnology.SUBSTANCE: invention relates to biotechnology, applied microbiology and can be used for use as a producer of aromatic compounds. Strain of microalgae Chlorella vulgaris Beyerinck SMP-1802153, capable of synthesis of a mixture of aromatic substances similar to resinoid of oakmoss, deposited in collection of microalgae IPPAS under registration number Chlorella vulgaris Beyerinck IPPAS C-2019.EFFECT: invention increases output of mixture of aromatic substances similar to resinoid of oakmoss.1 cl, 1 tbl, 3 ex

Recombinant plasmid dna pndctr1 coding hybrid polypeptide gst-ndctr1, escherichia coli bl21 (de3)/pndctr1 bacteria strain - producer of hybrid polypeptide gst-ndctr1 and hybrid polypeptide gst-ndctr1, making chelates of copper, silver and platinum ions // 2603092
FIELD: biotechnology.SUBSTANCE: invention relates to biotechnology and can be used for recombinant production of CTR1. Method comprises producing plasmid DNA pNdCTR1 coding hybrid polypeptide GST-NdCTR1, consisting of the N-terminal polypeptide fragment of glutathione-S-transferase (GST), connected via thrombin hydrolysis site with the polypeptide fragment of the N-terminal domain of a high-affinity human copper CTR1 (NdCTR1) importer. Size of the plasmid DNA is 5149 bps, the plasmid DNA comprises BamHI-XhoI - pGEX-4T-1 fragment with size of 4951 bps and SLC31A1 gene fragment with size of 198 bps, encoding the N-terminal domain of human CTR1 protein. Nucleotide NdCTR1 sequence and GST sequence are in the same reading frame. E.coli BL21(DE3) cells are transformed with the produced DNA pNdCTR1 to obtain an E.coli BL21 (DE3)/pNdCTR1 producer strain.EFFECT: invention allows producing hybrid polypeptide GST-NdCTR1 making chelates of copper, silver and platinum ions.3 cl, 4 dwg, 1 tbl, 5 ex
Strain of lactic bacteria enterococcus durans - producer of lactic acid and antibiotic substances // 2603087
FIELD: biotechnology.SUBSTANCE: invention relates to biotechnology. Disclosed is a strain of Enterococcus durans kos 37 b No. 19 with high antagonistic capacity with respect to opportunistic and pathogenic microflora. Strain is deposited in VKPM under registration number VKPM V-11960. Strain of Enterococcus durans VKPM V-11960 can be used in food industry, as well as a base for producing a probiotic starter.EFFECT: invention reduces duration of fermentation of milk.1 cl

Isolated strains of lactobacillus plantarum dsm mcc1 23881 and lactobacillus gasseri mcc2 dsm 23882 microorganisms, and use thereof // 2603059
FIELD: biotechnology.SUBSTANCE: group of inventions refers to the strains of Lactobacillus plantarum DSM MCC1 23881 and Lactobacillus gasseri MCC2 DSM 23882 microorganisms and use thereof as antioxidant proteolytic ingredients for food product or food additive preparation. Strains generate conjugated linoleic acid (CLA), hydrogen peroxide (H2O2) and nitrogen monoxide (NO). Food products and food additives containing the above strains together or separately, are hypoallergenic, allergy and reduced irritation symptoms of lower urinary tract in males adult individuals, accompanying benign prostatic hyperplasia, and associated with oxidative stress and inflammation. Invention also discloses methods for production of food product or food additive.EFFECT: group of inventions relates to biotechnology and can be used in food industry.13 cl, 6 dwg, 13 tbl, 6 ex

Strain of bacteria escherichia coli-succinic acid producer (versions) and method for preparing succinic acid using this strain // 2603004
FIELD: biotechnology.SUBSTANCE: disclosed versions of the bacteria Escherichia coli, which are succinic acid producers. Escherichia coli bacterium is modified so that the genes ackA, pta, poxB, ldhA, adhE, ptsG are inactivated in it, expression of genes galP and glk is strengthened, and bacterium possessing activity of pyruvate carboxylase. Also disclosed a version of bacteria Escherichia coli, having inactivated genes ackA, pta, poxB, ldhA, adhE, ptsG, icIR reinforced by expression of genes galP and glk, and activity of pyruvate carboxylase. Also disclosed a version of bacteria Escherichia coli, having inactivated genes ackA, pta, poxB, ldhA, adhE, ptsG, enhanced by expression of genes galP and glk, enhanced by expression of genes aceE, aceF and lpdA, and activity of pyruvate carboxylase. Also disclosed a version of bacteria Escherichia coli, having inactivated genes ackA, pta, poxB, ldhA, adhE, ptsG, icIR, enhanced by expression of genes galP and glk, enhanced by expression of genes aceE, aceF and lpdA, and activity of pyruvate carboxylase. Also disclosed a version of bacteria Escherichia coli, having inactivated genes ackA, pta, poxB, IdhA, adhE, ptsG, pflB, enhanced by expression of genes galP and glk, enhanced by expression of genes aceE, aceF and lpdA, and activity of pyruvate carboxylase. Also disclosed a version of bacteria Escherichia coli, having inactivated genes ackA, pta, poxB, ldhA, adhE, ptsG, pflB, icIR, enhanced by expression of genes galP and glk, enhanced by expression of genes aceE, aceF and lpdA, and activity of pyruvate carboxylase. Disclosed a method for preparing succinic acid with using the above versions.EFFECT: group of inventions provides higher yield of succinic acid.19 cl, 2 tbl, 2 ex

Strains of streptococcus pneumoniae (versions) and method of producing protective protein fraction, possessing intraspecific immunogenic activity // 2601158
FIELD: medicine.SUBSTANCE: present group of inventions relates to immunology. Streptococcus pneumoniae strains are deposited in the State collection of pathogenic microorganisms of pathogenicity of III-IV groups of the Federal state budgetary institution “Scientific Center for Expertise of Means of Medical Application” of the Ministry of Health of Russia under registration numbers 296, 297, 298. These strains have intraspecific cross-protective activity and can be used to produce protein-containing fractions. Disclosed is a method for producing protein-containing fractions from the above strains to develop pneumococcal vaccines. For this purpose the strain is cultured, microbial cells are separated by microfiltration and inactivated by dimethyl ketone with subsequent water extraction and ultrafiltration to obtain a protein fraction with molecular weight of 30-100 kDa and cool dehumidification.EFFECT: obtaining effective preparations with intraspecific immunogenic activity with respect to the respective serotypes of S.pneumoniae, which can be used as a antipneumococcal vaccine of a wide spectrum of action.4 cl, 1 dwg, 4 tbl, 1 ex

Composition of bacterial strains, mixture for bioremediation and using said composition for removal of contaminants from soil, as well as method of soil cleaning from contaminants // 2601155
FIELD: biotechnology.SUBSTANCE: group of inventions can be used for soil cleaning from contaminants. Composition of 11 bacteria strains capable to metabolize aromatic nitro compounds and resistant to antibiotics and heavy metals is proposed. Composition is deposited in Collection of Industrial Microorganisms IAFB - of Institute of agricultural and food biotechnology in Warsaw No. KPP 2041p. Method of soil treatment using vaccine containing composition of strains is proposed.EFFECT: group of inventions provides effective bioremediation of soil, contaminated with aromatic nitro compounds, heavy metals and antibiotics.24 cl, 1 dwg, 12 tbl, 4 ex

Strain of candida albicans varietas stellatoidea for producing diagnostic allergen // 2601123
FIELD: medicine.SUBSTANCE: strain of Candida albicans var. stellatoidea deposited in the State collection of pathogenic microorganisms of III-IV pathogenicity groups of FSBI “Scientific Center for Expertise of Means of Medical Application” of the Ministry of Health of Russia under registration numbers 295 produces allergens having sufficient immunogenic properties.EFFECT: invention can be used for producing a diagnostic allergen.1 cl, 1 tbl, 1 ex
 
2550871.
Up!