Indexing scheme associated with subclasses c12c-c12q and or c12s, relating to micro-organisms (C12R)

C   Chemistry; metallurgy(315514)
C12R              Indexing scheme associated with subclasses c12c-c12q; or c12s, relating to micro-organisms(2819)
icroencapsulated bacterial consortium for gluten degradation in fermented dough and method for obtaining said fermented dough // 2628314
FIELD: food industry.SUBSTANCE: microencapsulated bacterial consortium for gluten degradation contains Lactobacillus plantarum ATCC 8014, Lactobacillus sanfranciscensis ATCC 27652 and Lactobacillus brevis ATCC 14869, encapsulating agents, prebiotics selected from the group including polydextrose, inulin and agave syrup, and trehalose in combination with a proteolytic enzyme of bacterial origin and a proteolytic enzyme of fungal origin commonly used for baking. The method for obtaining a consortium for gluten degradation comprises the stages of separately reacting each strain of lactic acid bacteria, separately cultivating each activated strain in a liquid culture medium until each strain reaches a predetermined concentration, removing for each strain the culture medium excess for concentrating the microorganisms, preferably by centrifugation, with obtaining granules, separately resuspending the granules for each strain in a suspension of the saline solution and adjusting to a predetermined volume, mixing the required amounts of each strain and adjusting to a final volume, dissolving the encapsulating agents in water at suitable proportions of dissolved dry substances from 20 to 30%, adding prebiotic and trehalose, inoculating the mixture of the three strains of lactic acid bacteria with obtaining approximately 1010 CFU of said mixture of three strains of lactic acid bacteria per gram of the encapsulated powdery substance, and spray drying with the inlet temperature of 110 to 160°C and the outlet temperature of 60 to 80°C, at a feed rate of 20 to 50 ml/min. Invention also relates to fermented dough, the method for its obtaining, and sweet baked products obtained from the fermented gluten-free dough.EFFECT: group of inventions allows to obtain a consortium which is stable and quickly activated.17 cl, 4 tbl, 6 ex
ethod for assessment of cholera vibrio biofilms sensitivity to antibacterial drugs // 2628098
FIELD: biotechnology.SUBSTANCE: method is proposed for assessment of cholera vibrio biofilms sensitivity to antibacterial drugs, including biofilm production on glass cylinders. Cylinders with the resulting biofilm are placed on agar in a Petri dish, antibacterial drug solutions at different concentrations are added in the middle of the cylinder. The results are taken into account after 18-20 h of incubation at 37°C by the presence or absence of cholera vibrios growth in the cylinders prints. Cholera vibrios biofilm sensitivity to antibacterial drugs is assessed by the activity index calculated by the formula: where AI is the antibacterial drug activity index; MICs is the maximum MIC of this antibacterial drug for sensitive strains in accordance with MUK 4.2.2495-09; MICb is MIC value of this antibacterial drug with respect to the biofilm of the strain under study. Biofilm sensitivity of the strain under study is directly proportional to the AI index.EFFECT: accuracy of evaluation of cholera vibrio strains biofilm sensitivity to an antibacterial drug.2 dwg, 4 tbl, 2 ex
Bacillus megaterium 2-06-ts1 microorganisms strain as photosynthesis and plant growth energy activator // 2627608
FIELD: biotechnology.SUBSTANCE: Bacillus megaterium 2-06-TS1 bacterial strain is deposited in the Russian National Collection of Industrial Microorganisms FGUPGosNIIGenetika under accession number VKPM B-12402.EFFECT: invention improves the energy of seed germination, plant germination, increases root growth, as well as chlorophyll b, carotenoids and the total amount of photosynthesis pigments in plant seedlings.3 tbl
ethod for water surface purification from oil pollution // 2627598
FIELD: biotechnology.SUBSTANCE: method comprises microbial preparation application to a water body; the preparation is based on a consortium of Acinetobacter sp. IB DT 5.1/1 and Ochrobactrum sp. IB DT 5.3/2 microorganisms with a titer of at least 108 CFU/ml in the culture fluid. The purification process is carried out by water body aeration.EFFECT: invention allows to reduce time and improve the efficiency of water bodies purification from oil pollution.2 ex

Bacillus pumilus strain and method for obtaining amikumacin a antibiotic with its application // 2627187
FIELD: biotechnology.SUBSTANCE: method for amikumacin obtaining involves cultivation of a Bacillus pumilus bacteria strain VKPM B-12548 on a liquid nutrient medium containing sucrose, glucose, yeast extract, K2HPO4, NaCl, MgSO4, (NH4)2SO4, FeSO4×7H2O, MnCl2×4H2O, CaCO3 and tap water at a predetermined ratio of components, followed by cultivation for 40-42 hours under aerating conditions. Cells from the culture liquid are precipitated by centrifugation to obtain a supernatant. Amberlite XAD-2 sorbent is added to the supernatant, followed by elution of amikumacin A with n-butanol-acetone-water system at a ratio of 1:1:1.EFFECT: invention allows to increase the yield of amikumacin A antibiotic.2 cl, 3 dwg, 1 tbl, 1 ex

Aneurinibacillus migulanus strain and its application for gramicidin s production // 2627182
FIELD: biotechnology.SUBSTANCE: invention can be used to produce gramicidin S using the strain of the Aneurinibacillus migulanus VKPMV-10212 producer.EFFECT: resulting strain allows production of gramicidin S in high yield.2 cl, 2 dwg, 2 ex

Bacterium strains lactobacillus rhamnosus, with a wide spectrum of antagonistic activity relating to pathogenic and conditionally pathogenic microorganisms // 2627166
FIELD: biotechnology.SUBSTANCE: strain of bacteria Lactobacillus rhamnoses 24 ds, which has antagonistic activity against pathogenic and opportunistic microorganisms, including against yeast fungi of the genus Candida, deposited in the All-Russian Collection of Industrial Microorganisms under the registration number VKPM V-12596 and can be used In the production of probiotic bacterial preparations, biologically active food additives, fermented and unfermented food products.EFFECT: improved strain properties.1 dwg, 7 tbl, 2 ex

Bacterium strains lactobacillus rhamnosus 7 ds, with a wide spectrum of antagonistic activity relating to pathogenic and conditionally pathogenic microorganisms // 2627165
FIELD: biotechnology.SUBSTANCE: strain of bacteria Lactobacillus rhamnosus 7ds, which has antagonistic activity against pathogenic and opportunistic microorganisms, including those with yeast fungi of the genus Candida, was deposited in the All-Russian Collection of Industrial Microorganisms under the registration number VKPM V-12598. The strain can be used in the production of probiotic bacterial preparations, dietary supplements, fermented and unfermented foods.EFFECT: improved strain properties.1 dwg, 7 tbl, 2 ex

Bacterium strains lactobacillus rhamnosus, with a wide spectrum of antagonistic activity relating to pathogenic and conditionally pathogenic microorganisms // 2627164
FIELD: biotechnology.SUBSTANCE: strain of bacteria Lactobacillus rhamnoses 1 ds, which has antagonistic activity against pathogenic and opportunistic microorganisms, including against yeast fungi of the genus Candida, deposited in the All-Russian Collection of Industrial Microorganisms under the registration number VKPM V-12597 and can be used in the production of probiotic bacterial preparations, biologically active food additives, fermented and unfermented food products.EFFECT: improved strain properties.1 dwg, 7 tbl, 2 ex

High-effective method for bacterial polysaccharide production, preparation of conjugate based thereon and immunogenic composition // 2627156
FIELD: biotechnology.SUBSTANCE: method for production of serogroup X Neisseria meningitidis capsular polysaccharide, its conjugate with a carrier protein and an immunogenic composition based on the conjugate for preparation of a vaccine against meningitis. The method is implemented using a fermentation medium comprising casaminoacid, an optimal strategy of nutrient solution addition and an improved process for polysaccharide isolation and purification, exclusive of any chromatographic methods. The polysaccharide and protein ratio in the conjugate is in the range of 0.2 to 0.6. Inventions allow to obtain purified serogroup X Neisseria meningitidis polysaccharide in a yield of 300 to 550 mg/l, average molecular weight of 400 to 550 kDa, yield of 60% to 65% at the purification stage and containing less than 0.5% of protein, less than 0.5% of nucleic acids and less than 5 EU/mg of endotoxins. X polysaccharide is adjusted to a size of 150 to 200 kDa and conjugated to a carrier protein.EFFECT: increased efficiency.9 cl, 4 dwg, 11 tbl
Bacterial strain tsukamurella tyrosinosolvens vkpm v-12342 - destructor of alkanes and biosas producer for cleaning territories, water areas and industrial waste from oil pollutants // 2626593
FIELD: biotechnology.SUBSTANCE: Tsukamurella tyrosinosolvens PS2 bacterial strain is able to utilise aliphatic hydrocarbons and produce biologically surface-active substances. The bacterial strain is deposited in the Russian National Collection of Industrial Microorganisms under accession number VKPM B-12342 and may be used for cleaning territories and water areas contaminated with oil hydrocarbons.EFFECT: increased efficiency of territories and water areas cleaning from oil contaminations.4 ex
Dense nutrient medium for cultivation and collection of biomass of plague microbe of ypestis ev vaccine strain // 2626568
FIELD: biotechnology.SUBSTANCE: nutrient medium for cultivation and growth of plague microbial biomass of the Y. pestis EV vaccine strain contains the enzymatic hydrolyzate of corn extract as a nutrient base, condensed, sodium chloride, 2-substituted 12-aqueous sodium phosphate, Mohr's salt, microbiological agar and drinking water at a specified ratio of ingredients.EFFECT: invention allows to increase the yield of biomass of the vaccine strain of plague EB microbe.3 ex

Kluyveromyces marxianus vkpm y-4290 yeasts strains applied for production of ethanol on cellulose catalytic hydrolysates // 2626544
FIELD: biotechnology.SUBSTANCE: Kluyveromyces marxianus C1 thermotolerant yeasts strain is able to produce ethanol. The yeasts strain is deposited in the Russian National Collection of Industrial Microorganisms under accession number VKPM Y-4290 and can be used to produce ethanol by fermenting sugars mixtures, being products of the one-stage catalytic hydrolysis-dehydration of microcrystalline cellulose.EFFECT: invention enables to increase the yield of ethanol.2 dwg, 4 tbl, 5 ex
ethod for bioindication of surface-active substances in aqueous solutions // 2626533
FIELD: biotechnology.SUBSTANCE: invention may be used for bioindication of water contaminated with surface-active substances (SAS). The method involves incubation of maxillopods Epischura baicalensis Sars from Copepoda class in the tested and reference solutions with the following examination thereof through the microscope. At the same time the presence of surface-active substances in water is indicated through the changes in shape, size and quantity of grumous saccule.EFFECT: increased effectiveness of bioindication of surface-active substances in water.3 dwg

Immobilized bioacatalyst for fumaric acid production // 2626528
FIELD: biotechnology.SUBSTANCE: immobilized biocatalyst is proposed for fumaric acid production. The biocatalyst is the cells of the mycelial fungus of the Rhizopus genus introduced initially as a spore into the polyvinyl alcohol cryogel and sprouted to form mycelium. At that, the spores are included into the cryogel in the process of its formation. Germination of spores was carried out in a medium containing glucose 100 g/l, yeast extract 5 g/l and tryptone 10 g/l, or margarine 10 g/l and corn stalk extract 5 g/l, or potato starch 50 g/l and yeast extract 10 g/l, together with salts - (NH4)2SO4 2.36 g/l, MgSO4×7H2O 0.2 g/l, ZnSO4×7H2O 0.07 g/l, K2HPO4×3H2O 1.0 g/l, or in a medium containing barley malt 20 g/l or sucrose 30 g/l, yeast extract 10 g/l and soybean flour 10 g/l. The immobilized biocatalyst formed as a result of spores germination has the following composition (wt %): mycelium biomass (dry weight) - 6.5÷16.3; polyvinyl alcohol - 2.1÷6.0; water - up to 100.EFFECT: biocatalyst is stable in acid medium, characterized by high productivity, provides an increased yield of the target product, the period of use in the batch process reaches 2100 h.2 dwg, 2 tbl
Bacillus amyloliquefaciens ops-32 bacteria stain for the production of complete biopreparate for protection of agricultural plants from phytopathogenic mushrooms, stimulation of their growth and increase in yield // 2625977
FIELD: biotechnology.SUBSTANCE: strain of bacteria Bacillus amyloliquefaciens OPS-32, which has antagonistic activity against phytopathogenic microorganisms, was deposited in the All-Russian Collection of Industrial Microorganisms under the registration number RCIM V-12464. The strain can be used to create a biopreparation of complex action for use in crop production, including organic agriculturing.EFFECT: invention allows to increase crop yields.3 dwg, 5 tbl, 6 ex
Immunoenzymometric test system for animals anaerobic enterotoxemia serum diagnostics and postvaccinal immunity stress control // 2625031
FIELD: veterinary medicine.SUBSTANCE: invention relates to the immunoenzymometric test system for the anaerobic enterotoxemia serum diagnostics of agricultural animals, caused by Clostridium perfringens (Cl. perfringens) bacteria, as well as to control the postvaccinal immunity stress. The presented immunoenzyme test system contains the specific antigens of strains Cl. perfringens No.28 (type A), No.1 (type B), No.392 (type C), No.213 (type D), obtained by sounding the bacterial cells at the ultrasonic disintegrator, then precipitating the endotoxin with the ammonium sulfate and the dialysis against the piped water. The antigens are the specific protein in the concentration of 8-10 mcg/cm3, adsorbed on the surface of polystyrene wells in the carbonate-bicarbonate buffer. Also, the set contains the control positive serum, obtained for the antigens of Clostridium perfringens of types A, B, C, D with activity in ELISA 1:6400-1:12800, the control negative serum, the antispecies conjugate, the potassium phosphate monosubstituted, the potassium phosphate dibasic, the sodium chloride, the chromogen (orthophenylenediamine), the hydrogen peroxide, the stop reagent and the panels for the enzyme immunoassay reaction establishing.EFFECT: test system allows to provide diagnostic of animals anaerobic enterotoxemia and determine the postvaccination immunity stress.2 tbl, 5 ex
Reassortant strain of rn2/66-human a (h7n2) influenza virus for neuraminidase antibodies identification in case of influenza infection and vaccination // 2625024
FIELD: biotechnology.SUBSTANCE: diagnostic strain of RN2/66-human A (H7N2) influenza virus was obtained by crossing the equine influenza A/horse/Prague/1/1956(H7N7) virus with the cold-adapted vaccine strain A/17/California/66/395(H2N2) based on the attenuation donor A/Leningrad/134/17/57(H2N2). The strain contains the neuraminidase of the influenza virus subtype N2 A/California/1/1966 (H2N2) and influenza A/horse/Prague/1/1956 (H7N7) hemagglutinin. The RN2/66-human A(H7N2) strain actively multiplies in developing chick embryos at the optimum temperature of 33°C, which allows to accumulate viral material for subsequent purification and concentration. The RN2/66-human A (H7N2) strain can be used for solid-phase inhibition of neuraminidase activity to analyse background values of serum antibodies to influenza neuraminidase N2 in the human population and their changes as a result of infection/vaccination.EFFECT: improved strain properties.2 dwg, 1 tbl, 2 ex

Recombinant escherichia coli strain - producer of xyloglucanase from aspergillus cervinus fungi and method for xyloglucanase microbiological synthesis based on this strain // 2625013
FIELD: biotechnology.SUBSTANCE: recombinant strain of Escherichia coli VKPM B-12683, a producer of GH12 family xyloglucanase encoded by the AsCeGH12b (SEQ ID NO 1) gene cloned from Aspergillus cervinus VKPM F-612 is proposed. The strain of Escherichia coli VKPM B-12683 was obtained by Escherichia coli BL21-CODONPLUS (DE3)-R1PL strain transformation with pET-AsCeGH12b (SEQ ID NO 3) plasmid. A method for microbial synthesis of GH12 family xyloglucanase encoded by the AsCeGH12b gene cloned from Aspergillus cervinus VKPM F-612 by Escherichia coli VKPM B-12683 strain culturing under aerobic conditions in a suitable nutrient medium to maximize the accumulation of the desired product is proposed.EFFECT: high level of target product biosynthesis.2 cl, 1 dwg, 4 ex

E. coli bl21 (de3)/ptev-tms strain - fusion protein trxtevrs-tms producer, destined for proteolytic cleavage with tumstatin anti-angiogenic peptide formation, human tumstatin fragment [l69k-95] derivative, and method for recombinant anti-angiogenic peptide production // 2625008
FIELD: biotechnology.SUBSTANCE: method for recombinant anti-angiogenic Tumastin peptide a - human tumstatin modified fragment [L69K-95] derivative with Ser-Gly-Ala-Met-Gly and Pro-Gly-Pro sequences attached to N- and C-termini, respectively, provides culturing of E. coli BL21 (DE3)/pTEV-TMS producing strain, destruction of cell biomass in the presence of protease inhibitors, cell lysate centrifuging and separation of cell supernatant containing fusion protein, fusion protein chromatographic purification on a column with an anion exchange sorbent, proteolytic cleavage by TEV-protease, chromatographic purification of the anti-angiogenic Tumastin peptide.EFFECT: invention provides high yield of anti-angiogenic Tumastin peptide.2 cl, 5 dwg, 3 ex
Strain salinibacterium amurskyense arc 14 vkpm ac-1993 - destructor of oil and oil products // 2624069
FIELD: biotechnology.SUBSTANCE: invention relates to biotechnology, microbiology, ecology, environmental protection. Salinibacterium amurskyense ARC 14 strain has the ability to destruction of oil and oil products in aqueous medium. Salinibacterium amurskyense bacteria strain is deposited in the Russian national collection of Industrial Microorganisms under registration number VKPM Ac-1993. Strain can be used for purification of water areas of water bodies, shore line contaminated with oil and oil products, at temperature from +4 to +20 °C and salinity of 30 g/l.EFFECT: invention increases efficiency of purification of water areas of water bodies from oil and oil products at +4 to +20 °C and salinity of 30 g/l.1 cl, 3 ex
Strain nocardia coeliaca arc12 vkpm ac-1990 is destructor of oil and oil products // 2624067
FIELD: biotechnology.SUBSTANCE: invention relates to biotechnology. Strain Nocardia coeliaca ARC 12 has oil-oxidizing ability. Nocardia coeliaca strain is deposited in Russian National Collection of Industrial Microorganisms under registration number VKPM Ac-1990. Strain can be used for cleaning of water areas of water bodies, coastal line contaminated with oil and oil products, at temperature from +4 to +20 °C and salinity of 30 g/l.EFFECT: invention increases degree of oil destruction in water reservoirs with high salinity in wide range of temperatures.1 cl, 3 ex
Strain arthrobacter rhombi arc 16 vkpm ac-1988 - destructor of oil and oil products // 2624063
FIELD: biotechnology.SUBSTANCE: invention relates to biotechnology, microbiology, ecology, environmental protection. Arthrobacter rhombi ARC 16 bacteria strain has the ability to destruction of oil and oil products. Arthrobacter rhombi bacteria strain is deposited in the Russian national collection of Industrial Microorganisms under registration number VKPM Ac-1988. Strain can be used for purification of water areas of water bodies, shore line contaminated with oil and oil products, at temperature from +4 to +20 °C and salinity of 30 g/l.EFFECT: invention increases efficiency of purification of water areas of water bodies, shore line from oil and oil products at +4 to +20 °C and salinity of 30 g/l.1 cl, 3 ex
Arthrobacter strain rhombi arc 15 vkpm as-1989 - destructor of oil and oil products // 2624061
FIELD: biotechnology.SUBSTANCE: invention relates to biotechnology, microbiology, environmental friendliness, environmental protection. Strain of bacteria Arthrobacter rhombi ARC 15 has ability of destruction of oil and oil products. Arthrobacter rhombi strain is deposited in Russian national collection of Industrial Microorganisms under registration number VKPM As-1989. Strain can be used for purification of water areas of water bodies, coast line contaminated with oil and oil products at temperature from +4 to +20 °C and salinity of 30 g/l.EFFECT: invention increases efficiency of purification of water areas of water bodies, coast line from oil and oil products at temperature from +4 to +20 °C and salinity of 30 g/l.1 cl, 3 ex
Strain cobetia marina arc 11 vkpm b-12350 is destructor of oil and oil products // 2624059
FIELD: biotechnology.SUBSTANCE: invention relates to biotechnology. Strain Cobetia marina ARC 11 has oil-oxidizing ability. Cobetia marina strain is deposited in Russian National Collection of Industrial Microorganisms under registration number VKPM B-12350. Strain can be used for cleaning of water areas of water bodies, coastal line contaminated with oil and oil products, at temperature from +4 to +20 °C and salinity of 30 g/l.EFFECT: invention increases degree of oil destruction in water reservoirs with high salinity in wide range of temperatures.1 cl, 3 ex
Strain nocardia coeliaca arc 1 vkpm ac-1991 is destructor of oil and oil products // 2624058
FIELD: biotechnology.SUBSTANCE: invention relates to biotechnology. Strain Nocardia coeliaca ARC 1 has oil-oxidizing ability. Nocardia coeliaca strain is deposited in Russian National Collection of Industrial Microorganisms under registration number VKPM Ac-1991. Strain can be used for purification of water areas of water bodies, shore line, contaminated with oil and oil products at +4 to +20 °C and salinity of 30 g/l.EFFECT: invention increases degree of oil destruction in water reservoirs with high salinity in wide range of temperatures.1 cl, 3 ex
Strain leucobacter aridicollis arc 6 vkpm as-1992 destructor of oil and oil products // 2624054
FIELD: biotechnology.SUBSTANCE: invention relates to biotechnology, microbiology, environmental friendliness, environmental protection. Strain of bacteria Leucobacter aridicollis ARC 6 has ability of destruction of oil and oil products. Leucobacter aridicollis bacteria strain is deposited in Russian national collection of Industrial Microorganisms under registration number VKPM As-1992. Strain can be used for purification of water areas of water bodies, coastal line contaminated with oil and oil products at temperature from +4 to +20 °C and salinity of 30 g/l.EFFECT: invention increases efficiency of purification of water areas of water bodies, coastal line contaminated with oil and oil products at temperature from +4 to +20 °C and salinity of 30 g/l.1 cl, 3 ex
Strain arthrobacter rhombi arc 8 vkpm as-1987-destructor of oil and oil products // 2624052
FIELD: biotechnology.SUBSTANCE: invention relates to the field of biotechnology, microbiology, ecology, environmental protection. Strain of bacteria Arthrobacter rhombi ARC 8 has the ability to destruction of oil and oil products. Strain of bacteria Arthrobacter rhombi is deposited in the Russian national collection of Industrial Microorganisms under registration number As-1987. Strain can be used for purification of water areas of water bodies, coastal line, contaminated with oil and oil products at temperature from +4 to +20 °C and salinity of 30 g/l.EFFECT: invention allows to increase efficiency of cleaning water area, coastal line contaminated with oil and oil products at temperature from +4 to +20 °C and salinity 30 g/l.1 cl, 3 ex
Strain cobetia marina arc 10 vkpm b-12349 is destructor of oil and oil products // 2624050
FIELD: biotechnology.SUBSTANCE: invention relates to biotechnology. Strain Cobetia marina ARC 10 has oil-oxidizing ability. Cobetia marina strain is deposited in Russian National Collection of Industrial Microorganisms under registration number VKPM B-12349. Strain can be used for cleaning of water areas of water bodies, coastal line contaminated with oil and oil products, at temperature from +4 to +20 °C and salinity of 30 g/l.EFFECT: invention increases degree of oil destruction in water reservoirs with high salinity in wide range of temperatures.1 cl, 3 ex
icrobiological specimen for increasing yield of agricultural crops in liquid form // 2624047
FIELD: biotechnology.SUBSTANCE: microbiological specimen for increasing crop yields consists of a mixture of a bacterial suspension concentrate of Micrococcus luteus PBT-1 bacteria strain and a suspension concentrate of Penicillium sp microscopic fungus strain. PBT-2, products of metabolism and a sterile nutrient medium with a final titre of viable cells in liquid Micrococcus luteus PBT-1 bacteria strain which is not less than 20.0×106 CFU/ml and Penicillium sp microscopic fungus strain. PBT-2 is not less than 10.0×103 CFU/ml.EFFECT: invention allows to increase crop yields.3 tbl, 2 ex
Probiotic bacterial strain (versions) and symbiotic composition containing strains for child nutrition // 2624043
FIELD: biotechnology.SUBSTANCE: strain of Bifidobacterium breve B2274 DSM 24707, Bifidobacterium breve B7840 DSM 24708 and Bifidobacterium longum subsp.longum B1975 DSM 24709 bacteria possessing antimicrobial activity against pathogens of E. coli, Salmonella enteriditis, Clostridium difficile and Campylobacter jejunii was proposed. A pharmaceutical composition having antimicrobial activity against E. coli, Salmonella enteriditis, Clostridium difficile and Campylobacter jejunii pathogens and containing a bacterial mixture is also provided. The said bacterial mixture comprises at least one bacterial strain selected from the group consisting of Bifidobacterium breve B632 DSM 24706, Bifidobacterium breve B2274 DSM 24707, Bifidobacterium breve B7840, DSM 24708 and Bifidobacterium longum subsp. longum B1975 DSM 24709. A food composition and a food additive containing the bacterial mixture are also provided. The bacterial mixture contains at least one bacterial strain selected from Bifidobacterium breve B2274 DSM 24707, Bifidobacterium breve B7840 DSM 24708 and Bifidobacterium longum subsp. longum B1975 DSM 24709.EFFECT: increased antimicrobial activity of the strain.14 cl, 5 dwg, 2 tbl

Bacterial strain, capable of oxalate metabolism // 2624033
FIELD: biotechnology.SUBSTANCE: strain of Lactobacillus paracasei spp. Paracasei LPC 09 DSM 24243 bacteria is proposed, which decomposes oxalic acid and/or its salts. The use of a pharmaceutical composition for prevention or therapeutic treatment of hyperoxaluria, urolithiasis, renal failure, cardiopathies, kidney stones, diverticulosis and diverticulitis is proposed. At that, the pharmaceutical composition comprises a bacterial composition comprising a plurality of cells of at least one bacterial strain, wherein at least one bacterial strain is Lactobacillus paracasei spp. paracasei LPC 09 DSM 24243. A food composition comprising a bacterial composition comprising a plurality of cells of at least one bacterial strain, wherein at least one bacterial strain is Lactobacillus paracasei spp. paracasei LPC 09 DSM 24243 is also provided.EFFECT: effective decomposition of oxalic acid or its salt in an amount of more than 60 percent.10 cl, 5 dwg, 2 tbl
Strains of bacillus cereus 875 ts microorganism as tools for plant productivity improvement // 2624032
FIELD: biotechnology.SUBSTANCE: bacillus cereus TS 875 strain has the ability to stimulate the growth of plants. Bacillus cereus strain is deposited in the Russian National Collection of Industrial Microorganisms of FSUE GosNIIGenetika under accession number VKPM B-12242, and can be used for stimulation of the physiological properties of plant, plant growth and photosynthesis.EFFECT: invention improves plant productivity.3 tbl

odified ras gene of escherichia coli bacterium, coding precursor of enzyme with penicillin g acylase activity, recombinant escherichia coli strain - producer of acylase penicillin g and method for microbiological synthesis of this enzyme // 2624022
FIELD: biotechnology.SUBSTANCE: nucleotide sequence of the gene is represented in SEQ ID NO: 17. This gene codes the precursor of penicillin G acylase enzyme consisting of the amino acid sequence of the signal peptide L-asparaginase Erwinia carotovora and structural elements of acylase penicillin G E.coli. The present invention also relates to a recombinant strain of E.coli BL21 (DE3)/pMD704. This strain is a producer of penicillin G acylase. The strain was obtained by introducing said modified ras gene within pSVH0107 vector into the recipient strain E.coli BL21 (DE3). The invention also relates to the process of microbiological synthesis of penicillin G acylase. The method includes the cultivation of E.coli BL21 (DE3)/pMD704 strain under suitable conditions.EFFECT: invention allows obtaining Penicillin G acylase enzyme with a high activity.3 cl, 5 dwg, 4 ex

New enzymes of methanol dehydrogenase from bacillus // 2624017
FIELD: biotechnology.SUBSTANCE: polypeptides are characterized by amino acid sequences set in SEQ ID NO: 2, 4, 6. The present invention also relates to nucleic acid molecules encoding these polypeptides. The nucleotide sequences of the said nucleic acid molecules are shown in SEQ ID NO: 1, 3, 5. The invention relates to expression vectors comprising these nucleic acid molecules. The invention also relates to prokaryotic host microorganism for expression of the said polypeptide alcohol dehydrogenases.EFFECT: invention produces new polypeptides from Bacillus methanolicus with alcohol dehydrogenase activity, including methanol dehydrogenase.9 cl, 13 dwg, 3 tbl, 18 ex
Haemophilus influenzae spb strain type b - highly active producer of capsular polyribosylribitolphosphate polysaccharide // 2624014
FIELD: biotechnology.SUBSTANCE: Haemophilus influenzae SPB strain type b, which is a highly active producer of capsular polyribosylribitolphosphate polysaccharide, is deposited in the National Collection of Pathogenic Microorganisms and Cell Cultures "GKPM - Obolensk" under number B-7884. The invention provides polyribosylribitolphosphate synthesis, 250-300 mcg/ml.EFFECT: minimum cultivation time.1 dwg, 1 tbl, 2 ex
ethod for estimation of cytotoxicity of burkholderia pseudomallei strains - melioidosis pathogen based on their influence on tetrahymena pyriformis ctr-formation activity // 2623175
FIELD: biotechnology.SUBSTANCE: method involves cultivation of Tetrahymena pyriformis on LB broth with antibiotics in the amount required for the experiment. Burkholderia pseudomallei is cultivated on LB medium. Burkholderia pseudomallei is combined with Tetrahymena pyriformis at a ratio of 100:1 to obtain a co-culture and incubated at 28°C in the climatic chamber during the period under study, with the count of the number of trophozoites and cysts formed in the Goryaev's chamber after preliminary disinfection with 10% formalin. Differences in the dynamics of Tetrahymena pyriformis cytogenesis in a co-culture with Burkholderia pseudomallei are revealed in comparison with the control. The cytotoxicity of the strains studied is determined by the quantitative index, which is calculated as the ratio of the number of cysts formed to the total number of tetrachimene cells per each time interval studied. In this case, strains with high cytotoxicity cause cysts formation and destruction at an earlier time compared to the strains with reduced virulence.EFFECT: invention allows to increase the method sensitivity.3 dwg, 2 tbl, 2 ex
icrobiological specimen for increasing yield of agricultural crops in liquid form // 2623156
FIELD: biotechnology.SUBSTANCE: microbiological specimen for increasing crop yields consists of a mixture of a bacterial suspension concentrate of Micrococcus luteus PBT-1 bacteria strain and a suspension concentrate of Penicillium sp microscopic fungus strain. PBT-2, metabolic products and sterile nutrient medium. The final titer of viable cells in the liquid form of specimen is not less than 5.0×106 CFU/ml.EFFECT: invention allows to increase crop yields.3 tbl, 3 ex

Dna-structure encoding modified version of protective antigene bacillus anthracis // 2622085
FIELD: biotechnology.SUBSTANCE: invention relates to an artificial gene, allowing to express in bacteria cells an isolated domain 1 of PAG protective antigen from Bacillus anthracis 55 VNIIVVIM. This gene is intended for intriduction into the chromosome of bacillus in composition of specific DNA-structure.EFFECT: invention allows to improve method of obtaining protective antigen of the anthrax pathogen Bacillus anthracis.2 dwg, 3 ex
Amycolatopsis orientalis strain - producers of ehremomitcin antibiotic and method for ehremomitcin production // 2621866
FIELD: biotechnology.SUBSTANCE: Amycolatopsis orientalis strain, and a method for ehremomitcin antibiotic production are proposed. Amycolatopsis orientalis strain with high activity of ehremomitcin antibiotic production is deposited in the Russian National Collection of Microorganisms of the Institute of Biochemistry and Physiology of Microorganisms named after G.K. Skryabin, RAS under accession number VKMAc-2717D. Method for ehremomitcin antibiotic preparation includes antibiotic sorption from the culture fluid to the carboxyl cation exchanger Purolite C104 or C106 in H+-form, antibiotic elution by ammonia solution, eluate concentration, followed by neutralisation and crystallization as by precipitation by means of isopropanol.EFFECT: high product yield.2 cl, 2 tbl, 4 ex
Application of the aspergillus awamori mycelial fungus as a recipient for the design of target enzyme producers // 2621279
FIELD: biotechnology.SUBSTANCE: invention relates to the use of the Aspergillus awamori mycelial fungus strain RCIM F-148 as a recipient for the design of target enzymes producers.EFFECT: invention allows the design of recombinant target enzymes producers, including producers of xylanases and mannanases.3 dwg, 2 tbl, 2 ex
Trichoderma asperellum strain - producer of hydrolytic enzymes and peptide metabolites and its application // 2620971
FIELD: biotechnology.SUBSTANCE: group of inventions refers to Trichoderma asperellum fungus strain VKPM F-1087 and its application as a promising drug for human antitumor cells activity suppression. Trichoderma asperellum strain VKPM F-1087 is a producer of hydrolytic enzymes and peptide metabolites possessing antibacterial, antitumor, fungicidal, enzymatic activities and can be used in biotechnology as well as in agriculture to inhibit phytopathogens.EFFECT: expanded spectrum of properties of the produced highly active strain metabolites.3 cl, ex 5
Nutrient medium for selective detection of pathogenic mannitol positive staphylococci // 2620965
FIELD: microbiology.SUBSTANCE: invention relates to sanitary and clinical microbiology and can be used for selective detection of pathogenic mannitol positive staphylococci in food products, clinical material and other objects. Nutritional medium contains pancreatic hydrolysate of casein with shallow degree of splitting, meat peptone, yeast extract, potassium phosphate, sodium chloride, mannitol, lithium chloride, glycine, red phenol, 2 % solution of potassium tellurite and agar in given ratio.EFFECT: invention increases sensitivity of nutrient medium to mannitol positive staphylococci.1 cl, 2 tbl, 5 ex
Recombinant plasmid pdualrep2 dna and strain transformed thereby for detection of substances and mixtures, inhibiting proteins biosynthesis and/or sos-response induction in bacteria // 2620074
FIELD: biotechnology.SUBSTANCE: genetic structure contains genes of two fluorescent proteins with distinct fluorescence spectra. Expression of one fluorescent protein gene is activated by stopped translation of the preceding genetically modified Escherichia coli tryptophan operon attenuator. Expression of the second gene is activated by de-repression of the preceding Escherichia coli sulA gene promoter. The genetic structure is a plasmid, transforming E. coli bacterial cells to obtain the E. coli tolC Δreporter strain.EFFECT: obtained strain allows efficient analysis of a large number of substances or mixtures to find antibacterial agents, while identifying those that inhibit protein biosynthesis or induce SOS-response to DNA damage.5 cl, 5 dwg, 1 tbl, 3 ex
Optimized recombinant protein encoding gene - human interferon alpha-17 analog // 2620073
FIELD: biotechnology.SUBSTANCE: nucleotide sequence encoding a recombinant protein - interferon alpha-17 analog, optimized for expression in E. coli cells by using codons optimal for the bacterium. The nucleotide sequence is cloned into pET30a and pET32a plasmids, which are used to produce E. coli producing strains. The invention allows expression of an interferon alpha-17 analog with an activity of more than 50% in the MDBK/VSV system of 2.1⋅109 Un/mcmol.EFFECT: possibility of obtaining of a recombinant protein - interferon alpha-17 analog.3 cl, 6 dwg, 3 tbl, 3 ex
A method for obtaining fermented vegetable raw material with crisp consistency // 2620062
FIELD: food industry.SUBSTANCE: method involves preparation of recipe components. Making ferment lactic acid bacteria, which are used as Lactobacillus casei and Lactobacillus plantarum in a ratio of about 1:1, and a fermentation of vegetable raw materials is performed. The cucumbers, red, Chinese, Peking, Sichuan, savoy, sheet, decorative, Brussels sprouts, cauliflower, broccoli or cauliflower can be used as plant raw materials.EFFECT: invention allows to expand the range used for the fermentation of vegetative raw material by maintaining a crispy consistency.5 ex

Cell and method of rhamnolipides production // 2619877
FIELD: biotechnology.SUBSTANCE: bacterial cell capable of forming at least one rhamnolipid and genetically modified in such manner that, compared with its wild type, it has an increased activity of two enzymes E1 and E2 or three enzymes E1, E2 and E3 and at least one enzyme E8 which catalyzes export of rhamnolipides from the cell into the environment is presented; at this enzyme E1 is capable of catalyzing the conversion of 3-hydroxyalkanoil-ACP via 3-hydrocyalkanoil-3-hydroxy alkane acid ACP into hydrocyalkanoil-3-hydroxy alkane acid, enzyme E2 represents rhamnosiltrasferaza I, and enzyme E3 - rhamnosiltransferaze II. A method for preparing rhamnolipides using said cell is presented.EFFECT: increase of rhamnolipides formation.12 cl, 5 tbl, 3 dwg, 28 ex
ethod for mycobacteria detection from surfaces // 2619220
FIELD: biotechnology.SUBSTANCE: method for mycobacteria detection from surfaces involves material collection from the surface using a swab moistened with saline and processing thereof by means of "Septustin" disinfectant in a 1% aqueous solution. At that, "Septustin" is mixed with a suspension of the studied material in a ratio of 1:4 for 30 minutes. Moreover, overall exposure incudes 15 minutes of centrifugation at 3000 rpm. The studied material is centrifuged for 15 min at 3000 rpm and the residue is washed with physiological saline twice by centrifugation under the same regime. Plating from the residue is carried out on a solid growth medium at the optimum temperature and time with subsequent colonies isolation and identification.EFFECT: invention improves the accuracy of laboratory research and reduces research time.12 tbl, 6 ex

Temperaturically intelligent mutant intein for the insoluble expression of the precursor of the target protein // 2619217
FIELD: biotechnology.SUBSTANCE: temperature-sensitive mutant PRP8 mini-intein from Penicillium chrysogenum insoluble for expression in Escherichia coli cells of the target protein precursor is provided. Mutant PRP8 mini-intein comprises a substitution of an amino acid residue leucine at position 93 to a proline residue, substitution of a cysteine residue at position 1 to an alanine residue and optionally contains a substitution of a cysteine residue at position 11 to tyrosine residue in the original sequence PRP8 mini-intein from Penicillium chrysogenum, consisting of 157 amino acid residues. When using a mutant intein combined and insoluble expression self-splitting ofpurified fusion protein from the refolding process that does not require use of proteases. The degree of processing for the modeling of SUMO target protein is more than 50%, and for neurotoxin CVIE - about 60%.EFFECT: improved properties.9 dwg, 8 ex
Pseudomonas libanensis b-3041d strain for soil and water media cleaning from petroleum hydrocarbons // 2619183
FIELD: biotechnology.SUBSTANCE: bacterial strain Pseudomonas libanensis VKM B-3041D, is proposed, intended for soil and aquatic ecosystems cleaning from petroleum hydrocarbons, including polyaromatic hydrocarbons and naphthenic compounds.EFFECT: strain is effective for oil-polluted soil and water media purification.1 dwg, 2 ex, 3 tbl
 
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