Fungi and culture media therefor (C12N1/14)

Trametes hirsuta strain - producer of ethanol // 2614263
FIELD: biotechnology.SUBSTANCE: Trametes hirsuta MT-24.24 basidiomycete strain has the ability to produce ethanol. Trametes hirsuta basidiomycete strain is deposited in the Russian National Collection of Industrial Microorganisms under accession number VKPM F-1288 and can be used in medicine and food industry.EFFECT: invention allows to obtain high quality alcohol containing no impurities such as acetone and isopropanol.1 tbl, 5 ex

Fungi strain from sordariomycetes class - producer of eremoxylarin a antibiotic // 2614126
FIELD: biotechnology.SUBSTANCE: ascomycetous fungi strain of Sordariomycetes INA 01108 class has the ability to produce eremoxylarin A antibiotic. Ascomycetous fungi strain of Sordariomycetes class is deposited in the Russian National Collection of Microorganisms of the Federal State Budget Institution Science Institute of Biochemistry and Physiology of Microorganisms named after G.K. Skryabin, RAS under accession number VKM F-4676D and can be used in medicine.EFFECT: invention allows to increase the yield of eremoxylarin A antibiotic.4 dwg, 2 tbl, 1 ex

System for cell culturing // 2612915
FIELD: biochemistry.SUBSTANCE: group of inventions relates to biochemistry. Cell culturing system, system for assessing intestinal effector agents, containing cell culturing system, are disclosed, methods of cell culturing, production of intestinal organoid and evaluation of treating of intestinal effector agents are also disclosed. Cell culturing system comprises device with liquid channel, liquid source, membrane in channel, intestinal epithelial cells on surface of membrane and bacterial cells adhering to intestinal cells. Method of cells culturing involves providing device with intestinal epithelial cells and bacterial cells, equipment it with culture medium at flow rate sufficient for removal of organic acids and unbound bacterial cells. Method for production of intestinal organoid includes delivery of cultural medium to system and cultivation of epithelial cells of intestine and bacterial cells in vitro. Evaluation method of treating intestine involves contact of intestinal epithelial cells or bacterial cells in system with potential intestinal effector agent and measurement of cell response.EFFECT: invention provides development in vitro model simulating mechanical, structural, absorbent, transport and pathophysiological properties of intestine.41 cl, 23 dwg, 1 tbl

New recombinant strain of filamentous fungi penicillium canescens cs15, producing cellulase clostridium thermocellum and method of its cultivation // 2612158
FIELD: biotechnology.SUBSTANCE: invention relates to a recombinant strain of filamentous fungi Penicillium canescens CS15 VKM F-4679D, secreting thermostable cellulase Cel48S from Clostridium thermocellum. The invention also discloses a method for culturing of the said strain in a particular nutrient medium. The said nutrient medium consists of 35 g/l of soybean hulls, 40 g/l of corn extract, 6 g/l of KH2PO4, 0.23 g/l of CaCl2, 0.3 g/l of MgSO4* 7H2O, 10 g/l of yeast extract and 10 g/l of wheat bran.EFFECT: invention allows efficient production of cellulase from Clostridium thermocellum in a heterologous host Penicillium canescens.2 cl, 6 dwg, 1 tbl, 4 ex
Penicillium vulpinum micromycetes strain f-1523 with antibacterial activity on anthrax agent bacillus anthracis // 2612150
FIELD: medicine.SUBSTANCE: invention relates to medical microbiology and biotechnology. Penicillium vulpinum micromycetes strain, having antibacterial activity on anthrax agent Bacillus anthracis, is deposited in State collection of pathogenic microorganisms and cell cultures (GKPM-Obolensk) under registration number F-1523. Fugate of cultural fluid of micromycetes strain Penicillium vulpinum F-1523 in dose of 100 mcl shows maximum activity in relation to Bacillus anthracis STI-1 on second day of cultivation.EFFECT: invention provides use of Penicillium vulpinum F-1523 in suppressing development of anthrax agent.1 cl, 3 tbl, 3 ex
Biological product for stimulating the growth of plants and their protection against phytopathogens on the basis of strains of trichoderma, strains of trichoderma for the production thereof (variants), method for producing a biological product on the basis of such strains // 2607785
FIELD: biotechnology.SUBSTANCE: group of inventions relates to biotechnology and can be used to create bioprotection of plants against phytopathogens and to stimulate their growth. Group of inventions comprises the strains of the fungus of Trichoderma longibrachiatum species (3 options); biological product for stimulating the growth of plants and their protection against phytopathogens on the basis of these strains and the method for producing a biological product. Strains of the Trichoderma longibrachiatum fungus are deposited at the ARRIAM FSBSI under registration numbers: RCAM 03324, RCAM 03323, RCAM 03325 respectively. Biological product contains spores and fragments of mycelium of above strains from 2×109 to 4×109 CFU/cm3; has antagonistic activity against phytopathogenic fungi of the Fusarium and Cladosporium species. Method of producing a biological product involves a two-stage joint cultivation of strains in a quantitative ratio of 1:1:1 consistently on two nutrient media for 4–5 days. This group of inventions provides increase in seed germination on 4–15 % and the increase of linear sizes of plants on 0.5–24 %.EFFECT: increased seed germination and the increase of linear sizes of plants.5 cl, 2 tbl, 5 ex

ethod of simulating processes biocorrosion disasters of thin-wall sealed shells made of aluminium-magnesium alloys at operation of spacecrafts and mock composition for its implementation (versions) // 2603797
FIELD: microbiology.SUBSTANCE: invention relates to applied microbiology and biocorrosion tests, namely, to methods for simulating processes of biocorrosion disasters of aluminium-magnesium alloys, used in aerospace engineering. Method for simulating processes of biocorrosion disasters of thin-wall sealed shells made of aluminium-magnesium alloys at operation of spacecrafts, in which test and reference samples of AlMg6 aluminium-magnesium alloys are produces, prepared test and reference samples are dried, test samples are sterilized, mock composition based on organic acid is prepared, which is applied on the sterilized test samples, and at least two samples are placed in Petri dishes, they are closed, reference samples treated with distilled water are also placed in Petri dishes, Petri dishes with test and reference samples are placed in different desiccators, bottom of which is filled with water to maintain moisture content of more than 90 %, desiccators are closed and maintained at room temperature, exhibit of test and reference samples is performed, then samples are extracted from Petri dishes, they are washed in running water, where test and reference samples are used with machined surface, and preparation of the simulation composition is performed by distilled water and one of organic acids selected from the following carboxylic acids: gluconic or malonic, obtained composition is applied on the test samples of AlMg6 aluminium-magnesium alloys with the help of the sprayer, after washing of samples in running water they are rinsed with distilled water and are dried, then, using raster electron microscope initial stages of biocorrosion and its type are estimated, chemical composition of the disaster surface of samples is researched, herewith, the exhibit of test and reference samples is performed for 30-45 days. Mock composition for modelling of biocorrosion disasters of thin-wall sealed shells of aluminium-magnesium alloys is described.EFFECT: increased reliability and life of structural materials in the presence of technophile microorganisms under operating conditions of sealed enclosure.3 cl, 9 dwg, 1 tbl

ethod for detoxification of white phosphorus using microorganism strain trichoderma asperellum vkpm f-1087 // 2603259
FIELD: biotechnology.SUBSTANCE: invention relates to biotechnology, specifically to a method for detoxification of white phosphorus in contaminated soil. Decontamination is performed by treatment of white phosphorus-contaminated soil with a strain of Trichoderma asperellum VKPM F-1087. Method enables detoxification of soil containing white phosphorus in concentration up to 1.0 wt%, exceeding the maximum allowable concentration 5,000 times.EFFECT: method enables detoxification of soil.1 cl, 1 dwg

Strain of candida albicans varietas stellatoidea for producing diagnostic allergen // 2601123
FIELD: medicine.SUBSTANCE: strain of Candida albicans var. stellatoidea deposited in the State collection of pathogenic microorganisms of III-IV pathogenicity groups of FSBI “Scientific Center for Expertise of Means of Medical Application” of the Ministry of Health of Russia under registration numbers 295 produces allergens having sufficient immunogenic properties.EFFECT: invention can be used for producing a diagnostic allergen.1 cl, 1 tbl, 1 ex

Lecanicillium muscarium fungus strain having insecto-acaricidal and antibiotic activity for fighting against sucking pests, fungal and bacterial diseases // 2598251
FIELD: agriculture.SUBSTANCE: invention relates to agricultural microbiology, in particular to protection of plants from pests and diseases. Lecanicillium muscarium MP-4 strain has insecto-acaricidal, as well as antibiotic activity to a complex of plant pathogenic fungi and bacteria. Strain is deposited in the State Collection of Microorganisms pathogenic for plants and their pests under the registration number of Lecanicillium muscarium G-033 VIZR and can be used for producing a preparation against a complex of sucking pests, mushroom and plant pathogenic fungi and bacteria.EFFECT: invention allows accelerating and increasing the death rate of sucking pests and mites and reducing the morbidity of plants caused by phytopathogenic bacteria and fungi.1 cl, 8 tbl, 9 ex

Strain paraphoma sp. producer of feosferid a // 2596928
FIELD: biotechnology.SUBSTANCE: invention relates to biotechnology, particularly, to production of biologically active substances and can be used for producing of feosferid A. Stain of mushroom Paraphoma sp., deposited in FGBNU VIZR at registration number VIZR 1.46, is proposed as a producer of a feosferid A.EFFECT: invention allows to raise output of feosferid A.1 cl, 2 ex

Strain of fungus mortierella alpina for obtaining highly active plant growth regulator // 2596922
FIELD: biotechnology.SUBSTANCE: disclosed is a strain of fungus Mortierella alpina VKPM F-1134 for production of a complex of fatty acids with predominance of arachidonic acid, having growth-regulating effect in relation to plants. Strain is isolated from rhizosphere of winter wheat grown in Iglinsky district of Republic of Bashkortostan. Strain synthesizes a complex of fatty acids, having physiological activity with respect to plants, among which there is not less than 50 % by weight of arachidonic acid.EFFECT: invention provides growth-stimulating activity on a wide range of agricultural crops, enhancing seed germination, promoting increase of vegetative mass of plants and improved formation of photosynthesis mechanism, increasing crop yield.1 cl, 1 tbl

ethod of producing protein biomass of basidium fungus pleurotus pulmonarius // 2588474
FIELD: biotechnology.SUBSTANCE: invention relates to biotechnology and can be used in production of protein food additive. It is a method of producing protein biomass by means of deep cultivation of Pleurotus pulmonarius PP-3.2 on starch-ammonia medium under conditions of aeration at pH 5.5-6.0 and 23-25 °C. It is based on mobilization of deep mycelium on a polymer grid made of high-pressure polyethylene with cell size of 2×2 mm. Separation of biomass and drying are carried out together with polymer carrier.EFFECT: invention simplifies the process of biomass producing.1 cl, 4 dwg, 2 tbl, 1 ex

Phoma ligulicola var.inoxydabilis boerema fungus strain having mycoherbicidal activity against common wormwood // 2588470
FIELD: agriculture.SUBSTANCE: invention relates to agricultural microbiology, particularly to biological control of unwanted (weed) vegetation by plant pathogenic fungi. Fungus strain Phoma ligulicola var. inoxydabilis Boerema, having high mico herbicide activity with respect to common wormwood, deposited in State collection of FGBNU VNII protection of plants under registration number VIZR 1.43 and can be used for control of common wormwood and related species of this plant.EFFECT: invention allows to reduce number of common wormwood.1 cl, 7 tbl, 3 ex

Culture base for producing culture medium for growing producers of daunorubicin // 2588374
FIELD: biotechnology.SUBSTANCE: invention can be used for submerged cultivation of actinomycetes of Streptomyces type, which are producer of anti-tumour antibiotic daunorubicin. Culture base for producing culture medium contains starch, soya flour, (NH4)2C4H4O6, (NH4)2SO4, CaCO3, K2HPO4, glucose, perfluorodecalin and sorbitan monostearate at specified component ratio.EFFECT: invention increases output of biomass of actinomycetes of Streptomyces genus and higher yield of daunorubicin.1 cl, 2 tbl

Nutrient medium for submerged cultivation of mycelium armillaria mellea // 2586483
FIELD: biotechnology.SUBSTANCE: invention relates to biotechnology and microbiology. Nutrient medium contains glucose, peptone, disubstituted potassium phosphate (KH2PO4), magnesium sulphate 7-aqueous (MgSO4×7H2O), soya bean oil and water at a given ratio.EFFECT: invention allows to reduce time required for cultivation of Armillaria mellea mycelium.1 cl, 1 tbl, 3 ex

ethod of producing compactin // 2585234
FIELD: biotechnology.SUBSTANCE: invention relates to biotechnology. Disclosed is a method of producing compactin, involving culturing Penicillium citrinum VKPM F-1099 strain on a culture medium. Obtained culture liquid alkalised to pH 12.0 at 30-50 °C for 2-3 hours. Mycelium is then separated. Method then includes performing sorption of compactin from native solution on a column with hydrophobic nonionic sorbent with elution thereof with aqueous solution of isopropanol. Compactin is extracted from obtained solution with butyl acetate with acidification to pH 3.0-5.0. Extract is purified with activated carbon. Compactin is concentrated and lactonised with partial evaporation of solvent at residual pressure of 10-14 kPa and heating to 75-95 °C in presence of a mineral acid. Method includes crystallising compactin-lactone from aqueous solution of isopropanol at room temperature and periodic stirring for 1-2 hours, then at a temperature of 6-10 °C for at least 4 hours. Product is then recrystallised.EFFECT: invention provides degree of lactonisation of compactin of not less than 99 % and obtaining a product with compactin content of not less than 98 %, sum of impurities of not more than 1 %, single maximum dimer impurity of not more than 5/10 % and output of product of not less than 65 %.1 cl, 3 ex

Industrial method of producing compactin // 2585233
FIELD: biotechnology.SUBSTANCE: invention relates to biotechnology. Disclosed is an industrial method of producing compactin. Method comprises culturing Penicillium citrinum VKPM F-1099 producer strain under conditions of aeration in a nutrient medium containing carbohydrate and nitrogen sources and mineral salts. Obtained culture fluid is acidified with mineral acid to pH 2.5-3.5 and held for 1-2 hours. Compactin is extracted from mycelium with butyl acetate in three steps. Extracts are purified with activated carbon. Purified extract is concentrated by distillation of butyl acetate at low pressure. Method then includes adding mineral acid and heating to 85-90 °C at low pressure to complete lactonisation of compactin. Compactin-Lactone crystallisation is carried out. Method then includes recrystallisation of compactin-lactone from aqueous solution of isopropanol at room temperature and periodic stirring for 1-2 hours, then at 6-10 °C for at least 4 hours with extraction of compactin. Product is obtained with compactin content of not less than 97 %, sum of impurities of not more than 1.0 %, single maximum dimer impurity of not more than 0.5 %.EFFECT: output of product is not less than 65 %.1 cl, 4 ex

Fungal strain of laetiporus sulphureus - producer of biologically active substances // 2584602
FIELD: biotechnology.SUBSTANCE: invention relates to biotechnology, particularly, to strains of fungi-producers of biologically active substances. Strain of Laetiporus sulphureus 3 having wide range of different biologically active substances, is deposited in Russian national collection of Industrial Microorganisms under registration number VKPM F-1188 and can be used in production of biologically active additives.EFFECT: invention increases output of true protein and content of carotenoids.1 cl, 3 tbl, 1 ex,

Biologically active preparation for protecting plants from pests, production method thereof, microcontainer for said preparation, method of making same and method of protecting plants from pests // 2581929
FIELD: agriculture.SUBSTANCE: group of inventions relates to protecting plants from pests. Disclosed is a method of producing biologically active preparation for protection of plants, biologically active preparation for protection of plants, method of producing micro containers for method implementation micro container for biologically active preparation and method of protecting plants from pests, where method involves activation of biologically active preparation and its application onto plants. Method of producing biologically active preparation involves introduction of entomopathogenic fungi and liquid phase in micro containers enclosed, decantation of liquid phase and drying micro containers together with entomopathogenic fungi spores. Method of making micro containers involves adding solvent polyisocyanate, stirring obtained solution in aqueous dispersion medium until micro emulsion state, production of microcapsules and heating of microcapsules with perforated shell and forming holes in it.EFFECT: invention enables minimising the effect of adverse factors on the active substance bio insecticides viability, improved consumer and commercial properties of biologically active preparations during mass use, wider range of bio insecticides application.10 cl, 16 dwg, 4 ex

ethod of producing biologically active additive // 2580050
FIELD: biotechnology.SUBSTANCE: invention relates to biotechnology and can be used in production of biologically active food additives, feed and medical purposes. Method includes two-step processing of mycelial biomass of fungus Aspergillus oryzae 12-84 (RCAM 01134) at natural pH value under action of intracellular enzymes. End product is obtained in form of enzymolysate. At 1st stage maintained at 48-50 °C for 1 hour 45 minutes - 2 hours 15 minutes, at 2nd step temperature is lowered to 38-40 °C for 2 hours 45 minutes - 3 hours 15 minutes and celloviridin is further used as a source of exo-β-glucanase. Temperature is then lowered to 30-32 °C and product is kept at said temperature for 5-6 hours.EFFECT: invention improves quality of obtained biologically active additive provide increase depth hydrolysis of polymers and cells get biologically active additive with a wide range of functional properties.3 cl, 4 tbl, 2 ex

Control of infestation by arthropods by application of entomopathogen and wax-containing particles // 2575617
FIELD: chemistry.SUBSTANCE: group of inventions relates to application of insecticidal complex particle for control of infestation by arthropods in grain storage places. Complex particle has diameter ≥10 mcm and includes wax particle, which has melting point ≥50°C, and spores of strain of entomopathogenic fungus Beauveria bassiana IMI 398548. Wax particle adheres to cuticle of at least one species of arthropods in granary and has weight, equal from 5 to 95 wt % of complex particle. Fungus spores adhere to hydrophobic wax particles and are capable of development on cuticle of at least one species of arthropods in granary. Also claimed is obtaining and application of dry powder composition, containing said complex particles and, optionally, additional excipients, for control of infestation by arthropods in places for grain storage.EFFECT: group of inventions provides effective control of number of arthropods in granary Mortality of arthropods constitutes 80-95% on 28-th day after exposure.11 cl, 5 dwg, 1 tbl

Strain of micromycete clonostachys candelabrum f-1466 characterised by antibacterial activity with reference to tularemia activator francisella tularensis 15/10 // 2571938
FIELD: biotechnologies.SUBSTANCE: strain of the micromycete Clonostachys candelabrum is offered which is characterised by antibacterial activity with reference to the tularemia activator Francisella tularensis 15/10. The micromycete strain is extracted from soil and deposited in the State collection of pathogenic microorganisms (SCPM-Obolensk) under the registration number F-1466. Filtrate water of cultural liquid of the micromycete strain Clonostachys candelabrum shows high activity with reference to Francisella tularensis 15/10 from the third day of cultivation. The maximum activity is achieved at the fourth day of cultivation. The diameter of the zone of suppression of growth of Francisella tularensis 15/10 at the fourth day is 45±5 mm.EFFECT: increase of activity.2 tbl, 2 ex

ycoplasma (mycoplasma hominis) inhibitor producer // 2569150
FIELD: medicine, pharmaceutics.SUBSTANCE: invention refers to biotechnology, microbiology. The strain Trichoderma harzianum Rifai VKPM (Russian National Collection of Industrial Microorganisms) F-180 is applicable as Mycoplasma hominis inhibitor producer for treating mycoplasma infections.EFFECT: invention enables inhibiting growth of Mycoplasma hominis.1 tbl

Fungal strain eremothecium ashbyi - producer of essential oil with fragrance of fresh flowers of rose // 2567675
FIELD: biotechnology.SUBSTANCE: strain Eremothecium ashbyi Guill. 503-ssa-II is a mutant, which was selected from the strain population Eremothecium ashbyi RNCIM F-36 (NRRL Y-1363) and deposited in the RNCM under the number RNCM F-4565D.EFFECT: fungal strain Eremothecium ashbyi RNCM F-4565D can be used as a producer of essential oil with the fragrance of fresh flowers of rose.1 tbl, 3 ex

Fungal strain eremothecium ashbyi - producer of essential oil similar to rose "otto" oil // 2567672
FIELD: biotechnology.SUBSTANCE: strain Eremothecium ashbyi Guill. 810-ssb-III is a mutant, it was selected from the strain population Eremothecium ashbyi RNCIM F-36 (NRRL Y-1363) and deposited in the RNCM under the number RNCM F-4566D.EFFECT: possibility to use the strain as a producer of essential oil similar to rose oil of Bulgarian origin.2 tbl, 3 ex

Strain of trichoderma citrinoviride bissett - producer of antibiotics-peptaibols with antifungal and antibacterial activity // 2564577
FIELD: biotechnology.SUBSTANCE: strain of Trichoderma citrinoviride RNCIM F-1228 is a producer of membrane-active antibiotics-peptaibols having antifungal and antibacterial activity. The strain was obtained by selection from a strain of Trichoderma citrinoviride TV4-1. The values of the diameter of the inhibition zone of growth of pathogenic fungi Candida albicans ATCC 2091 and Aspergillus niger INA 00760 with extracts of the culture fluid of the proposed strain are respectively 15±2 mm 25±2 mm. The value of the diameter of the inhibition zone of growth of methicillin-resistant strain of Staphylococcus aureus FDA 209R with the extract of the culture fluid of the proposed strain is 35±2 mm.EFFECT: improvement of the strain properties.2 dwg, 4 tbl, 5 ex

Shiitake mushroom strain lentinula edodes gna01 for producing fruit bodies // 2561471
FIELD: medicine.SUBSTANCE: group of inventions refers to microbiology, microbiology, particularly to edible mushroom growing. There are presented the shiitake mushroom stain Lentinula edodes (Berk.) Pegler GNA01 (account number: KCCM11135P) and a fruit body produced by culturing the above strain. The fruit body exhibits the new morphology and taste.EFFECT: advantage consists in producing the fruit bodies of shiitake mushroom possessing more solid flesh, tolerating the long storage well, which can be grown for the whole year.7 cl, 4 dwg, 3 ex

ethod of producing vaccine for prevention and therapy of trichophytosis in cattle // 2560572
FIELD: medicine.SUBSTANCE: invention relates to field of biotechnology and deals with method of producing vaccine for prevention and therapy of trichophytosis in cattle. Characterised solution includes inoculation of matrasses with culture of fungus Trichopyton verrucosum strain TF-130 L VGNKI on solid nutritional medium, placement of inoculated matrasses in thermostat in horizontal position with nutritional medium upward, collection of culture and realisation of fungal mass homogenisation, its connection with stabiliser in ratio 1:1 and prepackaging into flasks, with cultivation of fungus being realised within 8-10 days at temperature (27±0.5)°C.EFFECT: claimed invention makes it possible to reduce duration of vaccine production and increase viability of micronidia in finished vaccine.3 tbl

ethod of obtaining fermentation broth composition // 2560424
FIELD: chemistry.SUBSTANCE: invention relates to method of obtaining fermentation broth composition. Method comprises incubation of mixture, containing one or several fermentation broths, first organic acid component, second organic acid component, with pH from 3.5 to 5, temperature from 20°C to 50°C for 8-36 h. First organic acid component is selected from at least one of the following acids: acetic acid, acetic acid salt, formic acid, formic acid salt, propionic acid, propionic acid salt, or mixture of two or more of said acids in amount from 0.1 to 15 wt % of incubated mixture. Second organic acid component is selected from at least one of the following acids: benzoic acid, benzoic acid salt, cyclohexacarboxylic acid, cyclohexacarboxylic acid salt, 4-methylvaleric acid, 4-methylvaleric acid salt, phenylacetic acid, phenylacetic acid salt, or mixture of two or more of said acids in amount from 0.025 to 5 wt % of incubated mixture.EFFECT: invention ensures reduction of quantity of viable cells of filamentous fungi, which constitutes at least 4 log.35 cl, 8 dwg, 2 tbl, 2 ex

High-yield peptide antibiotics producing strain, preparation method and use thereof // 2560258
FIELD: chemistry.SUBSTANCE: invention relates to biotechnology, the high-yield antibiotics producing strain is Colephoma empetri, deposited in CGMCC with the accession number of CGMCC 4129. The obtained strain has stable genetic and producing properties, produces little impurities in fermentation, and is suitable for industrialisation.EFFECT: new strains for antibiotic production.7 cl, 2 tbl, 2 ex

Strain of micromycete trichoderma hamatum, having antibacterial activity against anthrax causative agent bacillus anthracis // 2558293
FIELD: biotechnology.SUBSTANCE: strain is deposited in the State collection of pathogenic microorganisms "SCIM-Obolensky" under the number F-11. The culture fluid centrate obtained as a result of culturing of said the strain on the liquid nutrient medium has high activity against anthrax causative agent. The zone of inhibition of growth of Bacillus anthracis is 20-25 mm and 35-45 mm when the culture fluid centrate of the said strain is used in an amount of 10 mcl and 100 mcl, respectively.EFFECT: strain of micromycete Trichoderma hamatum has antibacterial activity against anthrax causative agent Bacillus anthracis.4 tbl, 3 ex
Strain aspergillus niger-producer of citric acid // 2558228
FIELD: chemistry.SUBSTANCE: invention relates to biotechnology. The strain of fungus Aspergillus niger B-6 is the producer of citric acid. The strain Aspergillus niger is deposited in the joint Russian Collection of Agrocultural Microorganisms RAAS (RCAM) under registration number RCAM02149 and can be applied in the production of citric acid.EFFECT: invention makes it possible to increase the output of citric acid.2 tbl, 6 ex

Fungal strain aspergillus oryzae - producer of complex of proteinases and peptidases, nucleases, chitinase, beta-glucanase, mannanase and alpha-amylase // 2557300
FIELD: biotechnology.SUBSTANCE: strain Aspergillus oryzae 12-84, having a high level of synthesis of the complex of proteases and peptidases, nucleases, chitinase, β-glucanase, mannanase and α-amylase, is deposited in State Scientific Institution of All-Russian Scientific Research Institute of Agricultural Microbiology of the Russian Agricultural Academy under the registration number Aspergillus oryzae RCAM01134. The strain may be used to produce complex enzyme preparations with their subsequent application for hydrolysis of raw materials in the preparation of biocorrectors of food and feed, amino acid additives and biologically active additives with functional properties.EFFECT: invention enables to increase the yield of proteolytic, nuclease, chitinase, β-glucanase, mannanase and α-amylase activities.2 tbl, 2 ex

Fermentation medium and method of producing recombinant proteins // 2556120
FIELD: chemistry.SUBSTANCE: invention relates to biotechnology and specifically to a fermentation medium and a method of producing recombinant proteins using said medium. A fermentation medium for producing recombinant proteins selected from a group including GM-CSF, streptokinase and lipase, using microorganisms selected from a group including: E. Coli, Streptomyces sp. and Rhizomucor sp., is characterised by keeping concentration of urea or derivatives thereof in the range of 0.5 g/l to 2 g/l. The medium contains, per litre of water, basic salts in the following amounts: orthophosphoric acid (85%) 2.67-133.5 ml, calcium sulphate 0.093-4.65 g, potassium sulphate 1.82-91 g, magnesium sulphate-7H2O 1.49-74.5 g, potassium hydroxide 0.413-20.65 g, glycerine 4-200 g. The medium contains, per litre of water, trace elements in the following amounts: copper sulphate-5H2O 0.6-30 g, sodium iodide 0.008-0.4 g, manganese sulphate-H2O 0.3-15 g, sodium molybdate-H2O 0.02-1 g, boric acid 0.002-0.1 g, cobalt chloride 0.05-2.5 g, zinc chloride 2-100 g, iron (II) sulphate-7H2O 6.5-325 g, biotin 0.02-1 g, sulphuric acid 0.5-25 ml.EFFECT: invention increases the output of end products.6 cl, 27 dwg, 3 tbl, 16 ex
Fungal strain aspergillus foetidus - producer of complex of pectinases, β-glucanase, xylanase, cellulase, chitinase, mannanase and protease for destruction of polymers of plant and microbial raw material // 2549706
FIELD: biotechnology.SUBSTANCE: strain of micromycete Aspergillus foetidus 379-K-5-1 is proposed, producing a complex of pectinases, β-glucanase, xylanase, cellulase, chitinase, mannanase and protease for the destruction of polysaccharides of a plant and microbial material. The strain is deposited in the Departmental collection of useful microorganisms for agricultural purposes of the Russian Academy of Agricultural Science (RCAM) of the State Scientific Institution of the Russian National Research Institute for Agricultural Microbiology under the registration number of RCAM 01136. The advantage of the new strain is to obtain the more active complex of enzymes catalyzing the hydrolysis of polysaccharides, as well as endopolygalacturonase and pectinesterase. The strain Aspergillus foetidus RCAM 01136 is produced using efficient methods of selection and mutagenesis using UV irradiation and nitrosoguanidine from the known strain.EFFECT: invention enables to expand the range of the enzymes obtained, used for the destruction of polysaccharides.2 tbl

ethod of controlling undesirable herbaceous vegetation of class dicotyledones // 2543665
FIELD: agriculture.SUBSTANCE: invention relates to agricultural microbiology, in particular to protection of plants against invasive and perennial weed vegetation of class Dicotyledones. The suspension is used on the basis of vegetative mycelium of phytopathogenic micromycetes with chemical herbicides in sublethal doses. The plants are treated by spraying an aqueous solution of the herbicide with the active substance of glyphosate, and suspension of mycelium of foma-like fungi. Treatment is carried out at all stages of plant development, first with herbicide at the flow rate by glyphosate active substance of 0.02-0.32 kg/ha, then by a suspension based on mycelium fragments of foma-like fungi in a concentration of from 25 to 100 g/l. The consumption rate of the working fluid is from 50 to 300 l/ha. The proposed method enables to reduce 1.5-10 times or more the chemical load on the ecosystem, 1.5-4 times the infectious load (concentration of phytopathogenic fungi mycelium).EFFECT: method can be applied at all stages of plant development, without requiring additional protection of the cultivated crop.10 tbl, 5 ex

ethod of obtaining natural 9-decen-2-one by bioconversion of undecylenic acid by means of mould and application in perfumery as food fragrance // 2542388
FIELD: chemistry.SUBSTANCE: invention relates to method of obtaining 9-decen-2-one. Method includes cultivation of said mould, belonging to family Aspergillaceae or Mortierellaceae, addition of undecylenic acid as substrate in fermentation medium with consumption from 0.1 to 0.9 g/l/hour in presence of oil, substrate bioconversion in 9-decen-2-one, its extraction and purification. As oil, used is oil, selected from the group, containing traditional food oils or triglycerides, which contain short-chained fatty acids, or white oils.EFFECT: invention makes it possible to obtain 9-decen-2-one with output 8-16,5 g/l and high degree of purification 99%.13 cl, 1 dwg, 9 ex

Strain of macromycete trametes versicolor, used as producer for production of biologically active antifungal agent // 2541770
FIELD: biotechnology.SUBSTANCE: strain Trametes versicolor is proposed, used for production of antifungal agents against fungi of the genus Penicillium. The strain is deposited in the RCIM under the number F-1024.EFFECT: strain has high chitinase and fungicidal activity.4 dwg, 1 tbl, 5 ex
Producer of cucurbit bacterial spot agent (acidovorax citrulli) inhibitor // 2535983
FIELD: chemistry.SUBSTANCE: invention relates to biotechnology and can be used in agriculture. The Trichoderma harzianum Rifai strain is deposited in the Russian National Collection of Industrial Microorganisms under registration number VKPM F-180. The strain is capable of producing L-lysine-alpha-oxidase and can be used particularly as an inhibitor of cucurbit bacterial spot caused by Acidovorax citrulli bacteria.EFFECT: invention reduces loss of cucurbit crop.
ethod of solid-phase cultivation of aspergillus ochraceus to obtain proteinase - protein c activator of blood plasma // 2535872
FIELD: biotechnologies.SUBSTANCE: method for obtaining proteinase - protein C activator of blood plasma provides for solid-phase cultivation of fungus strain Aspergillus ochraceus BKM F-4104D on a substrate. The substrate contains the following components at a certain ratio: a solid substance or a carrier, glucose, amylum, hydrolysate of fish flour, peptone, NaCl, KH2PO4, MgSO4·7H2O, and water. As a solid substance or a carrier, vermiculite, polyurethane foam, silica gel or wheat middlings are used. Proteinase activity is up to 300 U/ml.EFFECT: invention allows obtaining a target product with improved activity.5 tbl, 15 ex

ethod of obtaining granulated product, granulated product for application in pesticidal composition (versions) and pesticidal composition containing thereof // 2534884
FIELD: chemistry.SUBSTANCE: group of inventions relates to biotechnology. A method of obtaining a granulated product includes growing filamentous fungi of a Monilialeae family, preferably Arthrobotrys conoides Dreschsler in a suitable liquid culture medium. The obtained culture of the filamentous fungus is mixed with at least one type of modified starch and starch flour, with the modified starch and modified flour being present in weight ratios, constituting from 30:70 to 60:40. Filling agents and in case of necessity nutritional substances are added to the obtained mixture with obtaining a paste. The granulation of the paste is carried out. The obtained granules are dried until the moisture level lower than 13% is achieved, preferably to the level constituting from 9 to 10%. The granulated product, obtained by the method described above, is used for the application in a pesticidal composition.EFFECT: group of inventions provides obtaining the target product with high dispersability and a high percent of survival of the filamentous fungi propagules.9 cl, 1 dwg, 4 ex
ethod of obtaining biological preparation for protection of plants against phytopathogens and nematodes based on genus trichoderma fungus strain and biological preparation obtained thereof // 2534213
FIELD: chemistry.SUBSTANCE: group of inventions relates to biotechnology. The method of obtaining a biological preparation for the protection of plants against phytopathogens and nematodes based on a genus Trichoderma fungus strain is realised by preparation of an inoculation material of the fungus strain, making the preparation in a liquid or friable form based on the inoculation material, and mixing the preparation with a mineral, organic or bacterial fertiliser.EFFECT: biological preparation, resulting from the method, possesses the high antagonistic activity with respect to a wide spectrum of phytopathogens and nematodes.6 cl, 5 ex

ethod of surface growing of microorganism on liquid culture medium and device for its realisation // 2532828
FIELD: chemistry.SUBSTANCE: group of inventions relates to biochemistry. Claimed is a device for surface growing of a microorganism on a liquid culture medium. The device includes a dish with a drain pan for growing of the microorganism, provided with a transport net freely dropped on its bottom. The transport net is made from a neutral material and is fastened by its ends to drive drums, which ensure lifting of the transport net from the dish bottom. The device also contains a reception device with a valve for the connection to a mixer-doser and supply of the liquid culture medium mixture with mother culture into the dish, rotary flaps, placed on butt ends of the dish for the regulation of air exchange in the dish, a drain device for pouring out the culture liquid, a pipe branch for the supply of a drying agent under the net, a sampling instrument and removable colour filters on lamps for the regulation of illumination, placed on side walls of the dish, a lid, which hermetically closes the dish, made with a possibility of its opening and/or removal, equipped with injectors for spraying the SAS solution above the surface of biomass growing in the dish and washing heads for the realisation of sanitary processing of the device. Also claimed is a method of surface growing of a microorganism on a liquid culture medium with the application of the claimed device.EFFECT: claimed inventions ensure growing of microorganisms on the liquid culture medium with the minimal application of manual labour.7 cl, 1 dwg, 2 ex
Wound-healing agent based on strain trichoderma harzianum rifai // 2528065
FIELD: biotechnology.SUBSTANCE: wound-healing agent is a concentrate of the culture liquid of strain Trichoderma harzianum Rifai deposited in the Russian National Collection of Industrial Microorganisms under the number of RNCIM: F-180, as a producer of L-lysine of alpha-oxidase, and can be applied as a wound-healing agent for skin lesions.EFFECT: invention enables to expand the range of means that provide wound healing of skin lesions.1 tbl, 2 ex
Inhibitor of andis virus of potato mottling // 2527899
FIELD: biotechnology.SUBSTANCE: invention relates to the use of the concentrate of the culture liquid of the strain Trichoderma harzianum Rifai, deposited in the Russian National Collection of Industrial Microorganisms under the number of RNCIM F-180 as an inhibitor of Andis virus of potato mottling.EFFECT: invention enables to reduce losses of potato from the plant infection with the Andis mottling virus.2 ex

Nutrient medium for growing filamentous fungi-dermatophytes from clinical material // 2527074
FIELD: biotechnology.SUBSTANCE: nutrient medium for growing filamentous fungi-dermatophytes from clinical material comprises glucose, bacteriological agar, meat peptone, casein hydrolyzate, yeast extract, sodium chloride, sodium carbonate, L-cysteine, thioglycolic acid and distilled water in a predetermined ratio of components.EFFECT: invention enables to reduce time of growing filamentous fungi-dermatophytes from clinical material.1 tbl, 2 dwg, 4 ex
ethod of restoring sensitive layer of biosensor // 2524438
FIELD: chemistry.SUBSTANCE: invention relates to biotechnology. The method involves treating the sensitive layer of a biosensor with solution of a common fraction of protease of hepatopancreas of king crab in a buffer comprising tris-HCl 50 mM, CaCl2 3 mM, NaCl 100 mM, pH 8.0 at solution temperature of 35-40°C. The process is carried out in multiple successive steps with a solution of a solution of a common fraction of protease of hepatopancreas of king crab in said solvent at temperature of 35-40°C followed by exposure. The sensitive layer of the biosensor is successively washed with dodecyl sulphate dissolved in the same buffer in concentration of 0.1% and then with water at each step. The treatment process is carried out three times.EFFECT: invention reduces the duration of the process.2 cl
Antiviral preparation obtaining method and antiviral preparation // 2522880
FIELD: chemistry.SUBSTANCE: method of obtaining an antiviral preparation is carried out by preparation of an inoculation mycelium of basidiomycete Enokitake Flammulina velutipes (Curtis) Singer, preparation of a liquid nutritional medium, which contains water, vegetable oil and molasses as a carbon source, as a nitrogen source - corn flour, as mineral salts - potassium dihydrophosphate and magnesium sulphate, its sterilisation, inoculation of the sterile nutritional medium with the prepared inoculation mycelium, cultivation of basidiomycete in it in an aerobic conditions; the obtained submerged culture is divided into basidiomycete biomass and a culture liquid, from which a clot is separated by addition to the latter of ethyl alcohol, which is pressed, dried and crushed with obtaining the antiviral preparation under specified conditions.EFFECT: method makes it possible to simplify technological process, increase the output of the antiviral preparation, possessing higher activity.2 cl, 1 tbl, 4 ex
Strain of filamentous fungus aspergillus oryzae - producer of maltogenic alpha-amylase // 2514224
FIELD: biotechnology.SUBSTANCE: strain of fungus Aspergillus oryzae Amy T-52-3-21 produces maltogenic α-amylase, and it is deposited in the All-Russian Collection of Microorganisms Institute of Biochemistry and Physiology of Microorganisms n.a. GK Scriabin RAS under the number F-4476D. The strain is made on the basis of strain Aspergillus oryzae of All-Russian Collection of Microorganisms F-3927D using the genetic engineering methods. Activity of α-amylase at 120 h of growth of the strain is 600-640 units/ml.EFFECT: higher level of activity of maltogenic α-amylase.1 tbl, 2 ex
 
2551357.
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