Including condition or time responsive control, e.g. automatically controlled fermentors (C12M1/36)

Bioreactor for growing methane-recycling microorganisms // 2607782
FIELD: biochemistry.SUBSTANCE: invention relates to biochemistry. Disclosed is a bioreactor for growing methane-recycling microorganisms with possibility of using methane-containing gas and oxygen-containing gas as substrates for cell growth. Bioreactor is a vertical cylindrical housing with a cover, bottom and central circulation pipe. In upper part of housing of bioreactor on opposite sides there are two closed sectors. Closed sectors form an external reaction volume, each sector is equipped with pipes to feed gaseous substrate for discharge of gas-liquid dispersion medium and to feeding a liquid stream into lower part of sector. Discharge pipe is connected with central circulation pipe, liquid stream feed pipe is connected with pipe for extraction of culture liquid from bottom of bioreactor. In each closed sector there is a mixing device with a logical device.EFFECT: higher efficiency with simultaneous reduction of power consumption, as well as possibility of making a bioreactor in proposed design of different volume.13 cl, 1 dwg

ethod for implementation of enzymatic processes and device therefor // 2596924
FIELD: biochemistry.SUBSTANCE: device for enzymatic processes and method for implementation of enzymatic processes using said apparatus. Apparatus comprises fermentation vessel with heat exchange jackets for liquid phase and solid-phase processes, aerator, buffer tank, transfer chamber, control device, pipelines with controlled valves and nozzles. Fermentation vessel for performance of solid-phase processes is equipped with temperature sensor, fermentation vessel for liquid-phase processes is equipped with pH sensor and temperature sensor. Fermentation vessel for liquid phase and solid-phase fermentation processes are interconnected with bypass pipeline, buffer tank is equipped with heat exchange jacket and coil. Gate chamber is equipped with bacterial filter of gate chamber supercharging and liquid level sensor. Upper cavity of gate chamber by means of pipelines with valves is interconnected with upper and lower cavity of buffer tank and bacterial filter for supercharging of gate chamber, and lower cavity is interconnected with liquid-phase fermentation tank and drain pipes fermentation products and sewer drain.EFFECT: invention ensures intensification of fermentative processes due to optimisation of conditions of enzymatic reactions.2 cl, 1 dwg

Bioreactor with membrane device for gas supply of microorganisms // 2596396
FIELD: biochemistry.SUBSTANCE: invention relates to biochemistry. Proposed is a bioreactor with a membrane device for gas supply of microorganisms. Bioreactor comprises a cylindrical housing, a cover, a bottom, a heat exchanger, a gas distributor, an aerator, a methane collector, tubular gas-permeable polymer membranes, branch pipes for the working liquid and gas flowing, an analyzer of exhaust gases CH4, O2, CO2, sensors of pH and T°C, electronic air flow and carbon dioxide meters, methane pressure regulator and enzymatic process controller. Gas distributor is equipped with guide plates and an aerating air diffuser, herewith it is made as a multichannel gas-lift pump. Methane collector is equipped with perforated holes for passage of liquid-gas mixture through the gas distributor. Separator of exhaust gases from liquid products of fermentation is equipped with working fluid level sensor and a pipeline connecting the lower part of the separator with the bottom part of the bioreactor.EFFECT: higher efficiency and explosion safety of bioreactor are provided.3 cl, 1 dwg

ethod of processing organic wastes and biogas plant therefor // 2595426
FIELD: biochemistry.SUBSTANCE: disclosed is a biogas plant and a method of processing organic wastes. Biogas plant comprises a receiving tank, biomass preprocessing unit, feed pipe, drain pipe, gas holder, a compressor, a group of concurrently operating bioreactors. Inlet pipes of bioreactors are connected to feed pipe through a system of distribution pipelines, outlet pipes of bioreactors are connected with drain pipe via a drain pipeline system. Gas discharge pipes of bioreactors are connected to gas holder via gas pipeline system, wherein drain pipe is equipped with a hydraulic pump and is connected to a drain vessel via a hydraulic lock. Each of bioreactors comprises bearing elements with populated microorganisms. Bearing elements are cylindrical frames. Cylindrical frames are maintained at a distance of 3-6 cm from each other. Method involves preparation of biomass, feeding prepared biomass into a group of single-type bioreactors, creating descending partial streams of biomass and maintaining constant level of biomass in bioreactors.EFFECT: invention increases amount of processed raw materials and increases specific capacity per unit volume of bioreactor.8 cl, 3 dwg

ethod of converting lignocellulose material into organic acid // 2592684
FIELD: biotechnology. SUBSTANCE: invention relates to biotechnology. Disclosed is a method of converting lignocellulose in an organic acid. Method comprises pretreating lignocellulose material with an alkaline agent comprising containing calcium or magnesium cations in presence of water at a temperature of pretreatment from 20 to 115 °C. Aqueous suspension of pretreated material is obtained. At least part of suspension is fed into a fermentation zone. Method includes enzymatic hydrolysis and fermentation to produce broth containing insoluble lignocellulose, precipitated and dissolved calcium or magnesium salt of an organic acid and an enzyme. Enzymatic broth is unloaded, followed by separation of a liquid phase containing dissolved salt of an organic acid, and a solid phase containing insoluble lignocellulose and a precipitated salt of an organic acid. At least part of liquid phase is recycled to pretreatment step with alkali, and/or to fermentation zone. EFFECT: invention enables to obtain a product in highly concentrated form with high output and purity. 16 cl, 1 dwg

ethod and process line for production of nisin // 2585521
FIELD: biotechnology.SUBSTANCE: group of inventions relates to production of nisin. Disclosed is method and process line for microbiological production of nisin. Method involves culturing of nisin producer in fermenter using nisin producer strain Lactococcus (Streptococcus) lactis, followed by extraction of nisin. Components of nutrient medium represented by brine after drying of dairy milk whey, milk whey, rinsings obtained after process equipment washing at dairy factories, as well as megaterin. Culturing is carried out using automatic pH-stating and thermal regulation, nisin extraction is carried out by acidification with subsequent nisin extraction by centrifugation. Process line contains fermenter with spherical bottom and cover. Fermenter is equipped with bubbler, jacket, mixer and automatic pH-stating and temperature controlling system. Inputs of fermenter are aligned with nutrient medium and seed material feed systems, and output by culture fluid is interconnected through extraction, centrifugation, ultrafiltration and nano filtration units with nisin preparation drying unit.EFFECT: simultaneously with production of nisin inventions provide recycling of previously not used milk production wastes.17 cl, 1 dwg

Fermentation apparatus for methane-assimilating microorganisms // 2580646
FIELD: biochemistry.SUBSTANCE: invention relates to the field of biochemistry. Disclosed is a fermentation plant for methane-assimilating microorganisms. Plant comprises a column fermenter and two reactors. Fermenter comprises a housing, an exhaust gas outlet pipe, a tube for feeding nutrient salts, process water, food components and seed biosuspension, a pipe for inlet of gas-liquid flow into fermenter from reactor and a pipe for outlet of biosuspension from fermenter into reactor. Each reactor comprises a sampling tube for gas-liquid flow in fermenter, a disc mixer with a drive, a pipe for feeding biosuspension from fermenter into reactor. First reactor comprises a pipe for feeding methane-containing gas, second reactor comprises a pipe for feeding oxygen-containing gas. Fermenter additionally contains temperature maintenance means, means of maintaining pH of medium, means of maintaining level of dissolved oxygen, exhaust gas analyser. Mixer drive in each reactor is equipped with power transducer connected via logical device controller with flow rate regulators of incoming gas flows and with control valves of supply of liquid in each reactor.EFFECT: invention reduces power consumption on process of aerobic fermentation, high degree of usage of oxygen and methane, increased efficiency, as well as possibility of making efficient industrial plants with volume of up to 100-400 m3.12 cl, 1 dwg

ethod of production of biomass of photoautotrophic microorganisms // 2577150
FIELD: biotechnologies.SUBSTANCE: method of production of a biomass of photoautotrophic microorganisms is offered. The method includes the preparation of liquid inoculum in an inoculator at continuous lighting and aeration by carbon dioxide with the subsequent supply of the obtained culture into the fermenter. Cultivation of the biomass in the fermenter is performed with the film-size flow of liquid in transparent tubes with simultaneous lighting by a lamp and aeration by carbon dioxide and supply of the ready culture of microorganisms into the ready culture collector. From the collector the biomass is supplied to the spray dryer where it is dried by warm air and the ready-made product is obtained in a powdery form with the content of solids of 95…97%.EFFECT: increase of the yield of the biomass, decrease of specific energy consumption, ensuring ecological safety, improvement of quality and increase of periods of storage of the finished product.1 dwg

ethod of producing biogas and apparatus therefor // 2561461
FIELD: chemistry.SUBSTANCE: group of inventions relates to biochemistry. A method and an apparatus for producing biogas from organic substances are disclosed. The method includes feeding a substrate into a container with a mixing mechanism, measuring rheological properties of the fermentation substrate in the region near impellers of the mixing mechanism. The measurement result is compared with a given value which is the desired value for a specific apparatus. The apparatus for producing biogas from organic substances comprises a container, a feeding system, a mixing mechanism, a sensor for measuring rheological properties of the fermentation substrate and a control unit.EFFECT: larger amount of the converted fermentation substrate and the generated amount of methane gas.18 cl, 2 dwg

ethod and apparatus for producing biogas // 2561460
FIELD: chemistry.SUBSTANCE: group of inventions relates to biochemistry. A method and an apparatus for producing biogas from organic substances are disclosed. The method includes feeding a substrate into a container with two mixing mechanisms with impellers. A common area is created in the container for mixing the fermentation substrate. The average speed of the fermentation substrate and/or average viscosity of the fermentation substrate in the mixing area are determined. The measurement data are sent to a control unit and controlled quantitative values are varied by the control unit. The apparatus comprises a container, a feeding system, a sensor for monitoring the process and mixing mechanisms, a control unit and mixing mechanisms with impellers. The impellers enable to generate horizontal streams of the contents of the container.EFFECT: larger amount of the converted fermentation substrate and the generated amount of methane gas.20 cl, 3 dwg

edical waste collection device // 2560578
FIELD: medicine.SUBSTANCE: what is presented is a medical waste collection device. The device comprises a load device and a former configured to be mounted in a tabletop hole. The load device comprises a branch pipe and a receiving unit configured to take the shape of a rectangle with rounded short sides in the former. The load device is thin-walled and single-space. The receiving unit of the load device belongs within the former, whereas the branch pipe is configured to be mounted in a waste vessel.EFFECT: laboratory assistant's safety when handling and recycling extremely hazardous biological materials, and reagents.4 dwg

ethod of growing colonies of microbial cells and device for its realisation // 2522005
FIELD: medicine.SUBSTANCE: group of inventions relates to biotechnology. Claimed is a method of growing colonies of microbial cells on a surface of a porous plate. The method includes supply of a nutrient solution from bottom to top through the porous plate into zones of growth of colonies of the microbial cells on its upper surface, supply of a suspension of the microbial cells onto the upper surface of the porous plate, creation of controlled conditions for the colony growth, performing observation of the colony growth, separation of the grown colonies of the microbial cells from the zones of growth and their transfer into external means of identification. The nutrient solution is supplied into the zones of growth of the colonies of the microbial cells by creation of a pressure difference between the hole input and output. Holes are made in the plate from an anode aluminium oxide orthogonally to its large plane and are topologically coded. The said zones of growth are formed in them in the form of porous membranes. The porous membranes are located at the same level as the upper surface of the plate or with formation of a hollow and do not pass the microbial cells. After supply of the nutritional solution, the suspension of the microbial cells of a specified concentration is supplied onto the upper surface of the plate until their homogenous distribution is achieved. Between the zones of growth on the surface of the plate a film, preventing attachment of the microbial cells, is formed. Separation of the grown microcolonies from the zones of growth is performed by hydroblow. A hydroblow is directed from the side of the input of cylindrical holes of the plate and spreads along them and farther through the pores of the porous membranes with force, which does not destroy the microcolonies but is sufficient for their separation from the growth zones. Also claimed is a device for growing the colonies of the microbial cells by the claimed method.EFFECT: providing conditions of automation of processes of the nutrient solution supply and processes of separation and transfer of the grown colonies, possibility of integration into miniature portable devices, and application in laboratories on a chip and provision of the device portability.6 cl, 14 dwg, 4 tbl, 2 ex

Device for obtaining nano-sized metal particles // 2518246
FIELD: chemistry.SUBSTANCE: invention relates to field of biotechnology. Claimed is device for obtaining nanoparticles by reduction of metals from initial salts in presence of cultivated cells of microorganisms. Device includes control computer (1), connected with it electronic block of regulation and control (2) of all functional units and blocks of fermenter (3), pH-stabilising block (4) with pH sensor (5) and hoses for supply of titering solutions by pumps (6, 7), block (8) for regulation of redox-potential of culture mixture, provided with redox sensor (9), independently controlled pumps (10, 11) for introduction of initial solutions of metal salts, reducing agents and growth factors into fermenter (3), block (12) for regulation of dissolved oxygen level with sensor pO2 (13), pump (14) for supply of growth substrate, block (15) for measurement of optic culture density with application of optic fibre sensor (16), block (17) for measurement of spectral characteristics of culture mixture with application of optic fibre sensor (18), isolated with impermeable for cells membrane with pore size 100-250 nm, block (19) for thermoregulation of fermenter (3), equipped with temperature sensor (20), block (21) for regulation of culture mixture mixing, which brings into motion blade mixer (22), block (23) for regulation of culture mixture illumination in case of cultivating phototrophic microorganisms and control of spectral parameters of submersible diode lamp (24), block (25) for ultrafiltration of sampled culture mixture with sterilising membrane with pore size 100-250 nm with possibility of output of only nanoparticle suspension from fermenter, condenser of output moisture (26), preventing loss of culture mixture.EFFECT: invention contributes to extension of arsenal of technological methods of obtaining nanoparticles of metals and makes it possible to achieve controllability of modes of nanoparticle formation.2 dwg, 3 ex

ethod to produce biomass of aerobic microorganisms // 2484129
FIELD: biotechnologies.SUBSTANCE: method includes inspection of an inoculator with process equipment for tightness, sterilisation of the inoculator with steam via an aeration device at the pressure of 0.20 - 0.25 MPa for 30…40 min., its filling with nutrient medium heated by steam to the temperature of 100°C. Then the temperature of the nutrient medium is increased to 121 - 123°C at steam pressure of 0.10 - 0.15 MPa, and the nutrient medium is maintained at these parameters for 15-60 min., afterwards the pressure in the inoculator is reduced down to 0.03…0.05 MPa. The nutrient medium is cooled down to cultivation temperature of 31…32°C with cold water with temperature of 7 - 10°C. After cooling of the nutrient medium, it is seeded with a seeding material with simultaneous mixing and aeration with sterile air. Cultivation of the produced liquid seeding culture is carried out at pH 4.2 - 4.5 and temperature of 31 - 32°C to achieve the phase of exponential growth for 12 - 14 hours. Then it is sent by means of displacement with sterile air from the inoculator into the prepared fermenter in the amount of 3…10% of the nutrient medium amount with its filling by 7/10 of its volume, and the microorganism culture is grown at fermentation temperature of 28 - 40°C for 96 - 120 hours with continuous aeration with sterile air, mechanical mixing and supply of warm water with temperature of 27 - 47°C into a heating jacket of the fermenter. After fermentation the cultural fluid with accumulated biomass is supplied into previously sterilised collectors of finished culture.EFFECT: increased yield of cultural liquid with accumulated biomass of aerobic microorganisms, reduced specific power inputs and provision of environmental safety at all stages of production.1 dwg, 2 ex

ethod of controlling processes of obtaining and drying enzyme preparations // 2480520
FIELD: chemistry.SUBSTANCE: enzyme preparations are obtained using a fermenter with a heating jacket for submerged culturing of microorganisms of the enzyme preparations with continuous aeration with compressed air and mechanical agitation at culturing temperature of 30…32°C on the entire volume of the fermenter; the culture liquid obtained in the fermenter is cultured to remove the residue and the filtrate of the culture liquid with moisture content of 92…95% is fed into a vacuum-sublimation drier, in which the desublimator used is a two-section evaporator of a vapour compression refrigerating machine, the working and spare section of which alternately operate in condensation and regeneration modes, respectively; wherein "hot" water is obtained by heating thereof in the condenser of the refrigeration machine using condensation heat of the coolant to temperature of 68…73°C, one part of which is fed into the heating jacket of the fermenter and the other is first fed for thawing the evaporator section operating in regeneration mode, and then the water cooled to temperature of 5…7°C is then removed from the evaporator section into a storage tank together with the liquid formed from the ice cover thawed on the surface of the cooling element in an amount of moisture evaporated from the enzyme preparation, and further, in closed cycle mode, fed in two streams, one of which is mixed with waste water after the fermenter before the condenser, and the second with "hot" water before the fermenter, wherein excess water from the recirculation loop is removed through the storage tank, followed by measurement of the culturing temperature in the fermenter, the flow rate and temperature of the water at the inlet of the heating jacket of the fermenter, the flow rate and moisture content of the filtrate of the culture liquid, residual pressure in the working volume of the vacuum-sublimation drier, the moisture content of the dried enzyme preparation, the flow rate and temperature of water vapour removed from the vacuum-sublimation drier into the working section of the evaporator, the boiling point of the coolant in the working section of the evaporator, the temperature of uncondensed vapour at the outlet of the working section of the evaporator and the level of water in the storage container; mass and heat flow of the mixture of cooled and "hot" water into the heating jacket of the fermenter is established by changing the ratio of flow rates thereof with adjustment of the culturing temperature in the fermenter; the measured values of moisture content and flow rate of the filtrate of the culture liquid are used to determine the power of the drive of the compressor of the refrigeration machine and the required residual pressure in the working volume of the sublimation drier by action on the power of the controlled drive of the vacuum pump with adjustment of the residual pressure on the final moisture content of the enzyme preparation; the flow rate and temperature of water vapour removed from the vacuum-sublimation drier into the working section of the evaporator, the temperature of uncondensed vapour at the outlet of the working section of the evaporator and the boiling point of the coolant in the working section of the evaporator are used to determine the current value of the coefficient of heat transfer from the water vapour to the coolant on the cooled surface of the working section of the evaporator, and upon achieving minimum permissible value of the coefficient of heat transfer, power of the drive of the compressor of the refrigeration machine is increased first, and the working section of the evaporator is then switched from condensation mode to regeneration mode while simultaneously switching to condensation mode the section operating in regeneration mode.EFFECT: improved quality of enzyme preparations by increasing accuracy and reliability of controlling the process parameters, high energy efficiency and environmental safety of production processes and vacuum-sublimation drying.1 dwg

ethod of culturing phototrophs and apparatus for realising said method // 2450049
FIELD: chemistry.SUBSTANCE: when culturing phototrophs, the culture fluid is stirred and aerated through agitation by moving cultivators back and forth in the horizontal plane at given temperature and pH values. The cultivators are illuminated with a pulsed light source with pulse duration of 0.00001-0.001 s and pulse spacing of 0.01-0.1 s. In the apparatus used, the culture fluid is illuminated with diodes located under transparent bottoms of vessels of the same geometric shape and powered by a pulse generator with controlled frequency and light pulse duration.EFFECT: group of inventions enables to reduce power consumption when culturing phototroph biomass.2 cl, 2 dwg, 2 ex

Continuous culture device with mobile vessel, which allows for taking most suitable cell versions // 2373273
FIELD: chemistry; biochemistry.SUBSTANCE: device for speeding up reproduction, through faster reproduction and/or increased reproduction output of living cells in a suspension or any cultured organisms through a natural selection process has a flexible sterile pipe 7 with culture medium. A system of movable clamping apparatus 3, 4, 5 divides the pipe 7 into separate zones, containing spent culture (downstream zone), growing culture (growth compartment) and fresh growth medium (upstream zone). In the device there is an apparatus 13 for moving gates and the pipe such that, part of the growth compartment and the culture associated with it can be shut off by clamping apparatus and separated from the growth compartment. That way, part of the pipe which contains unused medium can be linked with part of the culture and medium associated with it, already present in the growth compartment.EFFECT: realising a method with high reproduction output of living cells or cultured organisms.36 cl, 10 dwg

ethod and device for producing phycoerythrin with high optical density // 2315094
FIELD: biochemistry, in particular, methods and devices for producing coloring substances, possible use in food and cosmetic industry, and also during various biological research.SUBSTANCE: phycoerythrin protein pigment is produced by extraction from seaweed. It is extracted from seaweed, selected from a group including Galaxaura oblongata, Halymenia ceylanica, Helminthocladia australis and Porphyra dentate.EFFECT: phycoerythrin has high optical density.2 cl, 27 dwg, 2 tbl

Light diode matrix for lighting boards with cell holes and automated rack system for processing them // 2315093
FIELD: equipment for growing plant tissues.SUBSTANCE: in accordance to the invention, unit for accelerating growth of plant tissues contains a set of boards, forming matrices of holes. Each hole contains a tissue sample. Support for boards is provided by a rack which contains a set of vertically stacked shelves, containing one or more holding recesses, which forcedly move boards to given positions. Light for tissue samples is provided by a set of matrices of light diodes, mounted on mounting plates. Light diodes emit white light. Each mounting plate is supported by corresponding end comber-type rack connector, so that light diodes are close to boards, supported by shelves, positioned lower. Matrix of light diodes preferably matches matrix of holes, supported by a lower positioned shelf in fixed position, so that each light diode is centered above a corresponding hole.EFFECT: creation of high capacity system for processing samples of tissues which require light for supporting cell reproduction.7 cl, 7 dwg
ethod for proximate determination of non-sterility of broth for microorganism, animal cell and virus cultivation in bioreactors // 2307166
FIELD: biotechnology, in particular biopreparation production.SUBSTANCE: claimed method includes feeding of sterilized broth into presterilized inoculator or bioreactor equipped with means for redox-potential (eH) controlling, including eH electrode and microprocessor unit for controlling and adjustment of eH and pH measurement of redox potential value for 1 h under stirring and comparison of steady-state eH values with steady-state values. When redox-potential value deviates from steady-state value of broth redox-potential by 10 % said broth is recognized as non-sterile one.EFFECT: process of decreased cost.4 tbl, 4 ex

Installation of biological treatment of gases // 2156805
The invention relates to the field of purification of gases and can be used for exhaust gas cleaning in microbiological, paint, chemical, food, petroleum refining, and processing of agricultural products

Plant for production of bio // 2123525
The invention relates to biotechnology, namely biotechnologische equipment used in the processes of cultivation of microorganisms

The method of obtaining microaerophilic conditions for cultivation of campylobacter spp. // 2055885
The invention relates to working with enzymes or microorganisms with control conditions or time and can be used for clinical and laboratory diagnosis of campylobacteriosis etiological factors of acute intestinal diseases (OKZ)
 
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