With agitation means and with heat exchange means (C12M1/02)

Bioreactor for biochemical processes // 2610674
FIELD: biochemistry.SUBSTANCE: invention relates to biochemistry. Bioreactor for biochemical processes is disclosed. Bioreactor includes cylindrical vessel with device for medium mixing. Device for medium mixing consists of impeller, round partition and position control mechanism of impeller in height relative to surface of mixing medium. Impeller is horizontally fixed on vertical rod in upper part of vessel. Control mechanism includes rod vertically installed along vessel axis, connected by means of retainer with round partition for its rotation. Round partition is made with side diffuser holes at angle of 30–45° to circle and with top holes at angle of 45–60° to horizontal. Wherein above round partition additional impeller is placed on rod.EFFECT: invention provides simplified design and elimination of power consumption.1 cl, 2 dwg

Bioreactor for growing methane-recycling microorganisms // 2607782
FIELD: biochemistry.SUBSTANCE: invention relates to biochemistry. Disclosed is a bioreactor for growing methane-recycling microorganisms with possibility of using methane-containing gas and oxygen-containing gas as substrates for cell growth. Bioreactor is a vertical cylindrical housing with a cover, bottom and central circulation pipe. In upper part of housing of bioreactor on opposite sides there are two closed sectors. Closed sectors form an external reaction volume, each sector is equipped with pipes to feed gaseous substrate for discharge of gas-liquid dispersion medium and to feeding a liquid stream into lower part of sector. Discharge pipe is connected with central circulation pipe, liquid stream feed pipe is connected with pipe for extraction of culture liquid from bottom of bioreactor. In each closed sector there is a mixing device with a logical device.EFFECT: higher efficiency with simultaneous reduction of power consumption, as well as possibility of making a bioreactor in proposed design of different volume.13 cl, 1 dwg
Heat exchange module for use in chemical, pharmaceutical or biological reactor system // 2606011
FIELD: biochemistry.SUBSTANCE: invention relates to biochemistry. Disclosed is a heat exchange module for use in chemical, pharmaceutical or biological reactor. Heat exchange module comprises a flexible container with mixing system and a body with a central chamber to accommodate flexible container, wherein body is configured to be arranged in reactor vessel. Body with a central chamber comprises an outer surface configured to match shape of reactor vessel, heat-conducting surface configured for contact with flexible container, and a solid partition passing in central chamber, and heat exchanger on outer surface of said body. Heat exchanger comprises a channel for fluid medium circulation through which heat exchange fluid medium circulates around periphery of body and into channel, and from channel made in solid partition.EFFECT: invention provides for removal of large amount of heat generated by microbial cell cultures.6 cl, 7 dwg

ethod of obtaining polypeptide and method of producing reduced amount of glycoform g(0) and/or amount of glycoform g(1) of polypeptide // 2595407
FIELD: biochemistry.SUBSTANCE: invention discloses a method for obtaining a polypeptide and a method of obtaining a reduced amount of G(0) glycoform and/or increased amount of G(1) glycoform of polypeptide. Method for producing polypeptide involves cultivating a cell comprising a nucleic acid encoding polypeptide in a cultivation vessel and recovering polypeptide from culture medium or from cells. Method of producing a reduced amount of G(0) glycoform and/or increased amount of G(1) glycoform of polypeptide involves cultivating a cell containing polypeptide coding nucleic acid in a cultivation vessel and recovering polypeptide from culture medium or from cells. Acidic feed solution and an alkaline feed solution are mixed added to culture medium.EFFECT: invention enables to maintain viability of cultivated cells for a long period of time, reducing time during which nutrient compound is held at pH which reduces stability thereof, use of nutrient solution of solid substances, dispersion and hard-to-mix compounds/solutions, as well as reducing concentration of host cell protein in cell culture supernatant.12 cl, 20 dwg, 3 tbl, 3 ex

ethod for automated extraction with simultaneous purification of nucleic acids from several biological samples // 2595374
FIELD: biology.SUBSTANCE: invention relates to molecular biology. This is a method for automated extraction with simultaneous purification of nucleic acids from two or more biological samples. Method of extracting with simultaneous purification of nucleic acids is carried out in a single module on rotating platform separate for each sample. Besides the extraction with simultaneous purification of nucleic acids includes lysis step using dry reaction mixture of lysing buffer containing lysozyme, cell lysis step, microorganisms and viral particles using dry reaction mixture of lysing buffer containing chaotropic agent, proteolytic enzyme and detergent, adding alcohol to the obtained lysate, binding nucleic acids on a solidphase sorbent, dissolving the dry reaction mixture of washing buffer, washing of nucleic acids from impurities following with elution of nucleic acids.EFFECT: invention provides fast performance with high output of the procedure for simultaneous separating and purifying nucleic acids from two or more biological samples, as well as avoiding contact of personnel with infectious sample during extraction of nucleic acids.9 cl, 6 dwg, 1 tbl, 4 ex

ethod and process line for production of nisin // 2585521
FIELD: biotechnology.SUBSTANCE: group of inventions relates to production of nisin. Disclosed is method and process line for microbiological production of nisin. Method involves culturing of nisin producer in fermenter using nisin producer strain Lactococcus (Streptococcus) lactis, followed by extraction of nisin. Components of nutrient medium represented by brine after drying of dairy milk whey, milk whey, rinsings obtained after process equipment washing at dairy factories, as well as megaterin. Culturing is carried out using automatic pH-stating and thermal regulation, nisin extraction is carried out by acidification with subsequent nisin extraction by centrifugation. Process line contains fermenter with spherical bottom and cover. Fermenter is equipped with bubbler, jacket, mixer and automatic pH-stating and temperature controlling system. Inputs of fermenter are aligned with nutrient medium and seed material feed systems, and output by culture fluid is interconnected through extraction, centrifugation, ultrafiltration and nano filtration units with nisin preparation drying unit.EFFECT: simultaneously with production of nisin inventions provide recycling of previously not used milk production wastes.17 cl, 1 dwg

Fermentation apparatus for methane-assimilating microorganisms // 2580646
FIELD: biochemistry.SUBSTANCE: invention relates to the field of biochemistry. Disclosed is a fermentation plant for methane-assimilating microorganisms. Plant comprises a column fermenter and two reactors. Fermenter comprises a housing, an exhaust gas outlet pipe, a tube for feeding nutrient salts, process water, food components and seed biosuspension, a pipe for inlet of gas-liquid flow into fermenter from reactor and a pipe for outlet of biosuspension from fermenter into reactor. Each reactor comprises a sampling tube for gas-liquid flow in fermenter, a disc mixer with a drive, a pipe for feeding biosuspension from fermenter into reactor. First reactor comprises a pipe for feeding methane-containing gas, second reactor comprises a pipe for feeding oxygen-containing gas. Fermenter additionally contains temperature maintenance means, means of maintaining pH of medium, means of maintaining level of dissolved oxygen, exhaust gas analyser. Mixer drive in each reactor is equipped with power transducer connected via logical device controller with flow rate regulators of incoming gas flows and with control valves of supply of liquid in each reactor.EFFECT: invention reduces power consumption on process of aerobic fermentation, high degree of usage of oxygen and methane, increased efficiency, as well as possibility of making efficient industrial plants with volume of up to 100-400 m3.12 cl, 1 dwg

Device for cultivation of microorganisms // 2565557
FIELD: biotechnology.SUBSTANCE: device for cultivation of microorganisms is proposed. The device comprises a container, a mixing device inside it, made of agitators mounted on the shaft in tiers, the pipes for input of the nutrient medium, aerating gas and discharge of the exhaust gas and the finished product. The mixing device consists of three agitators. The upper agitator is made in the form of a solid disc with holes cut in it, at that the T-shaped blades with the wave-shaped slits in the visor are mounted on the disc. The central one is provided with vertical slits in the lateral sides, and the lower - in the form of a propeller.EFFECT: invention provides an intensification of the process of cultivation of microorganisms.4 dwg

ethod and plant for mixing of lignocelluloses with enzymes // 2564572
FIELD: process engineering.SUBSTANCE: invention relates to biochemistry. Invention discloses processing and liquefaction of lignocellulose. Processing comprises initiation of lignocellulose steam fracture reaction to get the suspension. Alkali-based material is added to said suspension. Enzymic solution is added to said suspension and mixed therewith to conduct the enzymic hydrolysis of said suspension. Liquefaction of lignocellulose comprises initiation of steam fracture reaction to get the suspension. Alkali-based material is mixed with said suspension to inject enzymes of enzymic solution to said suspension. Said suspension is mixed with addition of enzymic solution. System for liquefaction of lignocellulose comprises reactor for steam fracture, first and second screw conveyors, mixer communicated with outlet of second screw conveyor and reactor top for conversion of material thickness.EFFECT: accelerated liquefaction of lignocellulose.17 cl, 7 dwg

ethod of control of production of encapsulated enzyme preparations // 2556811
FIELD: biotechnology.SUBSTANCE: method comprises production of enzyme preparations by submerged cultivation of microorganisms of enzyme preparations with continuous aeration with sterile air and mechanical agitation throughout the volume of the fermenter; receiving the hot air in the condenser of the heat pump with discharge of one its part to the spray drier, and the other - for heating water; returning the exhaust air after the recuperative heat exchanger and evaporator of the heat pump to the condenser to form recirculation loop; alternate supplying the obtained culture fluid under pressure for filtration to two filters mounted in parallel with the countercurrent aqueous regeneration of the filter element, that alternately operate in the mode of separation with the discharge of the precipitate and regeneration; discharge of culture fluid filtrate with the content of solids of 5-8% first in the storage collector and then to the spray drier using a vapour-compression heat pump; draining the condensate from the evaporator, followed by feeding under pressure to the filter operating in the regeneration mode.EFFECT: invention enables to improve the quality and properties of the encapsulated enzyme preparations, to increase the shelf life of encapsulated enzyme preparations, as well as to improve energy efficiency.1 dwg, 2 ex
Cultivation method of photosynthesising microorganisms // 2550266
FIELD: biotechnologies.SUBSTANCE: invention proposes a cultivation method of photosynthesising microorganisms in a photobioreactor of a closed type with a working volume containing culture liquid. The method involves supply of a gas mixture containing carbon dioxide to the working volume of the photobioreactor, lighting of culture liquid from a source of artificial light and its mixing. Before the beginning of the cultivation process to the working volume of the photobioreactor to the culture liquid there added are granules of fluorescent dye with continuous afterglow in the amount of 10-30% of the culture liquid volume. Granules of fluorescent dye are provided with a transparent external cover from chemically and biologically inert material. At continuous or periodic sampling of the culture liquid from the working volume of the photobioreactor the granules of fluorescent dye are separated by means of a mesh filter.EFFECT: reduction of energy consumption for a cultivation process of photosynthesising microorganisms and increase of efficiency of the process.1 tbl, 2 ex

Bioreactor // 2540019
FIELD: agriculture.SUBSTANCE: bioreactor comprises a sealed vertical cylindrical tank mounted on the supports, the loading and unloading pipes, a pipe for removing biogas. The tank is provided with a bottom in the form of a truncated cone, covered with heating tape, the tank itself is also wrapped with heating tape and provided with a dome having the shape of a truncated cone facing with the smaller base upwards. Inside the tank the mixing device is mounted, which is a three-tiered paddle mixer made in the form of steel plates of rectangular cross section fixed crosswise on a vertical shaft (10), equipped immovably by fixed blades (11) in the form of flat steel plates of rectangular section, mounted parallel to the shaft. The upper paddles (7) of the mixer are mounted perpendicular to the shaft axis, each paddle is provided with a blade mounted perpendicularly on it, directed downwards, and the middle and lower paddles of the mixer repeat the shape of the tank bottom, the blades of which are attached to the paddles, and directed upwards. The middle paddles (8) of the mixer have one blade each, and the lower paddles (9) - three or more blades located with a gap over the entire surface of the paddle.EFFECT: increased productivity of the bioreactor and increase in the yield of biogas.3 dwg

odule bioreactor // 2532176
FIELD: agriculture.SUBSTANCE: tubular bioreactor is located horizontally and is divided by partitions-segments not reaching the bottom of the container, into three sections: loading 1, working 3 and unloading 13. In the loading section 1 of the bioreactor from above and in the upper part of the unloading section 13 the technological hatches 4 are made. The working section 3 is made of one or several modules. In each module of the working section 3 the stirrer 7 and the heat exchanger 6 are mounted. In the upper part of each module there is a technological hatch 4 and intake devices in the form of fittings for connecting the head flow meter and intake of biogas. All sections of the bioreactor are insulated. In the working section 3 between the modules the adjustable shut-off valve is mounted, which enables to isolate the modules hermetically from each other. In the unloading section 13 the stirrer-mixer is mounted.EFFECT: invention enables to create in the bioreactor safe and low energy intensity operation mode.4 cl, 5 dwg

Application of energy sources of sun and biomass in farming // 2520805
FIELD: power engineering.SUBSTANCE: invention relates to renewable energy sources to be used in farming. This method comprises application of the Sun renewable energy source and biomass of manure, biogas and slime as organic fertilisers in greenhouses and lawn-and-garden cultures. Firs, during April-October term, greenhouse is heated by water lines heated solely by sun energy to 60…70°C at continuous hot water circulation in closed circuit "greenhouse-reactor-greenhouse" when filtered manure filtrate is fed simultaneously with production of biogas to be discharged via communication pipelines into gas-holder and to household consumers and to boiler room heater. The latter allows circulation of hot water and regulation of constant temperature in said closed circuit "greenhouse-reactor-greenhouse". At a time, bioslime, organic fertilisers, are processed and fed via pipelines into greenhouse and garden-melons-gourds plots. Then, in November-March term two intermediate modes are used. First mode is used when greenhouse is not heated due to recultivation and other works and heated is solely the manure substrate by continuous circulation of hot water in closed water lines circuit "boiler room-reactor-boiler room". Proposed biomass is used like it is at the main mode while biogas is fed to household consumers. The latter allows circulation of hot water and regulation of constant temperature in said closed circuit "greenhouse-reactor-greenhouse". Second mode allows heating of both greenhouse and manure substrate by hot water circulation in closed circuits of "boiler room-reactor-green house" and "boiler room-reactor-boiler room". Processed biogas and bioslime are used similarly to the initial main mode. Then, the latter and two intermediate modes are used again.EFFECT: application of sun energy for production of biogas and fertilisers, non-polluting organic fertiliser.1 dwg

ethod to produce powdered ferment preparations // 2495122
FIELD: biotechnologies.SUBSTANCE: depth cultivation of microorganisms is carried out in two fermenters with heating jackets. Cultivation is carried out under continuous aeration with sterile air and mechanical mixing at temperature of 30…32°C in the entire volume of fermenters. Cultural fluid produced in two fermenters under pressure is alternately supplied for filtering in two parallel filters with counterflow water regeneration of a filtering element. Filters alternately operate in the mode of separation with residue removal and regeneration. The cultural fluid filtrate with moisture of 92…95% is sent into a spray dryer. The filtrate in the drying chamber contacts with the drying agent - hot air with temperature of 70…75°C. The powder of the ferment preparation is produced with moisture of 5…7%. Heating of air supplied into the spray dryer is carried out using a steam compression heat pump, comprising a compressor, a condenser, a heat control valve and an evaporator, which operate in the closed thermodynamic cycle. One part of heated air is sent into the spray dryer in the condenser of the heat pump. The other part of air is used as coolant for heating of water to temperature of 40…45°C. Water is then supplied into heating jackets of fermenters. The spent drying agent with temperature of 30…35°C and moisture content of 0.025…0.030 kg/kg is cooled in the evaporator to the temperature of 5…7°C and moisture content of 0.003…0.005 kg/kg. The drying agent with the spent coolant is again supplied into the condenser. The condensate produced in the evaporator is removed into the condensate collector with subsequent supply into the filter under pressure.EFFECT: production of a powdered target product with homogeneous particle size, increased energy efficiency.1 dwg, 2 ex

Biogas apparatus with metered microwave heating // 2490322
FIELD: chemistry.SUBSTANCE: invention relates to biotechnology and bioenergy and can be used in recycling livestock wastes and for producing biogas. The biogas apparatus has a vessel for preparing starting material, a pipe for feeding the prepared mass, a pump for feeding material, a pipe for removing the obtained sludge, a vessel for spent substrate, a system for treating, collecting, storing and processing biogas and a bioreactor, having at least three cylindrical concentrically arranged vessels: an outer vessel for conducting a psychrophilic step of anaerobic fermentation of the biomass, a middle vessel for the mesophilic step of fermentation and a central vessel for the thermophilic step of fermentation. The outer and middle vessels are linked to each other in the bottom part, and the middle and central vessels are linked to each other in the top part. The apparatus if equipped with a microwave radiator with radiation frequency of 2450 MHz, placed in the central vessel of the bioreactor such that it enables metered and uniform heating of the biomass on its entire volume. A bladed mixer is installed in each of the outer and central vessels.EFFECT: invention provides uniform heating on the entire volume of a bioreactor, high degree of decomposition of biological wastes and high output of biogas.1 dwg

ethod of controlling processes of obtaining and drying enzyme preparations // 2480520
FIELD: chemistry.SUBSTANCE: enzyme preparations are obtained using a fermenter with a heating jacket for submerged culturing of microorganisms of the enzyme preparations with continuous aeration with compressed air and mechanical agitation at culturing temperature of 30…32°C on the entire volume of the fermenter; the culture liquid obtained in the fermenter is cultured to remove the residue and the filtrate of the culture liquid with moisture content of 92…95% is fed into a vacuum-sublimation drier, in which the desublimator used is a two-section evaporator of a vapour compression refrigerating machine, the working and spare section of which alternately operate in condensation and regeneration modes, respectively; wherein "hot" water is obtained by heating thereof in the condenser of the refrigeration machine using condensation heat of the coolant to temperature of 68…73°C, one part of which is fed into the heating jacket of the fermenter and the other is first fed for thawing the evaporator section operating in regeneration mode, and then the water cooled to temperature of 5…7°C is then removed from the evaporator section into a storage tank together with the liquid formed from the ice cover thawed on the surface of the cooling element in an amount of moisture evaporated from the enzyme preparation, and further, in closed cycle mode, fed in two streams, one of which is mixed with waste water after the fermenter before the condenser, and the second with "hot" water before the fermenter, wherein excess water from the recirculation loop is removed through the storage tank, followed by measurement of the culturing temperature in the fermenter, the flow rate and temperature of the water at the inlet of the heating jacket of the fermenter, the flow rate and moisture content of the filtrate of the culture liquid, residual pressure in the working volume of the vacuum-sublimation drier, the moisture content of the dried enzyme preparation, the flow rate and temperature of water vapour removed from the vacuum-sublimation drier into the working section of the evaporator, the boiling point of the coolant in the working section of the evaporator, the temperature of uncondensed vapour at the outlet of the working section of the evaporator and the level of water in the storage container; mass and heat flow of the mixture of cooled and "hot" water into the heating jacket of the fermenter is established by changing the ratio of flow rates thereof with adjustment of the culturing temperature in the fermenter; the measured values of moisture content and flow rate of the filtrate of the culture liquid are used to determine the power of the drive of the compressor of the refrigeration machine and the required residual pressure in the working volume of the sublimation drier by action on the power of the controlled drive of the vacuum pump with adjustment of the residual pressure on the final moisture content of the enzyme preparation; the flow rate and temperature of water vapour removed from the vacuum-sublimation drier into the working section of the evaporator, the temperature of uncondensed vapour at the outlet of the working section of the evaporator and the boiling point of the coolant in the working section of the evaporator are used to determine the current value of the coefficient of heat transfer from the water vapour to the coolant on the cooled surface of the working section of the evaporator, and upon achieving minimum permissible value of the coefficient of heat transfer, power of the drive of the compressor of the refrigeration machine is increased first, and the working section of the evaporator is then switched from condensation mode to regeneration mode while simultaneously switching to condensation mode the section operating in regeneration mode.EFFECT: improved quality of enzyme preparations by increasing accuracy and reliability of controlling the process parameters, high energy efficiency and environmental safety of production processes and vacuum-sublimation drying.1 dwg

ethod to produce fuel and device for its realisation // 2480513
FIELD: oil and gas industry.SUBSTANCE: invention relates to the method to produce oil fuel, in which mixing is carried out and reaction of hydrolysis is done with water containing a ferment, which a hydrocarbon oil product, besides, water containing a ferment, is produced by means of mixing of a natural vegetable ferment, containing, at least lipase, in water. The natural vegetable ferment may additionally contain cellulase. The invention also relates to a device for production of oil fuel.EFFECT: increased efficiency of fuel, which is stable, and also suppression of hazardous substances formation.10 cl, 11 dwg, 1 ex

ethod for biological decontamination of liquid hydrocarbon-containing wastes and apparatus for realising said method // 2465218
FIELD: chemistry.SUBSTANCE: group of inventions relates to biotechnology. Disclosed is a method for biological decontamination of liquid hydrocarbon-containing wastes which are formed when cleaning natural gas and gas pipelines. A biopreparation of hydrocarbon-oxidising microorganisms is added to liquid hydrocarbon-containing wastes in amount of 1 kg of biopreparation per 10 kg of hydrocarbons. Water is simultaneously added to the working vessel to obtain a working suspension with volume ratio of wastes to water ranging from 1:4 to 1:50, depending on the initial concentration of hydrocarbons. Macroelements, trace elements and, optionally, an acidity regulator are then added. Decontamination is carried out for 12-15 hours while constantly stirring and feeding air. Temperature is kept in the range from 28°C to 36°C and pH is kept in the range 4 to 7. The decontamination product is then discharged from the working vessel, leaving not less than 25% of the volume of the working suspension. The whole decontamination cycle is repeated. The second and subsequent decontaminations are carried out without adding the biopreparation. Also disclosed is apparatus for biological decontamination of liquid hydrocarbon-containing wastes which are formed when cleaning natural gas and gas pipelines. The apparatus has a working vessel with process pipes, a mixer and a supply vessel mounted on top of the working vessel and linked to the working vessel by a connecting pipe. The mixer is fitted with an electric drive and is mounted with possibility of turning about a vertical axis. The working vessel is fitted with a level gauge, a loading bin for the biopreparation, a vessel for trace elements and macroelements, a vessel for the acidity regulator and a built-in heat-exchanger. The connecting pipe is fitted with a stop valve and a pipe for feeding water.EFFECT: shorter duration and higher efficiency of the decontamination process, wider field of using the method and the apparatus.4 cl, 2 dwg, 1 tbl

ethod of culturing phototrophs and apparatus for realising said method // 2450049
FIELD: chemistry.SUBSTANCE: when culturing phototrophs, the culture fluid is stirred and aerated through agitation by moving cultivators back and forth in the horizontal plane at given temperature and pH values. The cultivators are illuminated with a pulsed light source with pulse duration of 0.00001-0.001 s and pulse spacing of 0.01-0.1 s. In the apparatus used, the culture fluid is illuminated with diodes located under transparent bottoms of vessels of the same geometric shape and powered by a pulse generator with controlled frequency and light pulse duration.EFFECT: group of inventions enables to reduce power consumption when culturing phototroph biomass.2 cl, 2 dwg, 2 ex

Device for growing microorganisms // 2408720
FIELD: medicine.SUBSTANCE: device for growing microorganisms includes tower with detachable cover, nozzle of air exhaust, nozzle for drain of culture fluid and flushing waters, nozzles of nutrient medium, titrating agent and seed culture, multilevel disk mixer with drive installed in tower, aerator. Tower consists of two cylindrical reservoirs arranged one above other. Upper reservoir is arranged of larger diametre to contain lower reservoir installation in transport position for growing stock culture and has flange fixed in lower part with fixing bolts to seal reservoirs. Shaft of disk mixer is arranged as composite. Lower part of shaft is arranged as tubular and is installed in reservoir for growing stock culture. Upper part of shaft is arranged in the form of solid cylinder fixed in detachable cover of installation and inserted into lower tubular part of composite shaft of mixer with the possibility of displacement relative to each other. On upper cylindrical part of disk mixer shaft there is a disk with the possibility of displacement along shaft and its fixation by means of pins at specified height. Disks are arranged at the ends of lower tubular part on lower tubular part of disk mixer shaft. Installation is equipped with lifting mechanisms arranged under flange of upper reservoir to match reservoirs with each other in working position of installation.EFFECT: increased convenience of installation operation due to reduction of dimensions and possibility to use it in mobile complex of ecological biotechnology.2 dwg

Apparatus for suspension cultivation of tissue or microorganism cells // 2363729
FIELD: microbiology instruments.SUBSTANCE: invention relates to suspension cultivation of tissue or microorganism cells and can be used in food industry, biotechnologies and medicine. Proposed apparatus comprises cylindrical vessel with cover and device to aerate and mix suspension of mentioned biological substances. The said apparatus allows producing, on suspension surface, aerating gas swirled flow flowing along the vessel edges, potential vortex rate field being located at the vessel edges and axial countercurrent in near-axis zone. Note that the pressure difference between the vortex edges and centre varies from 10 to 2000 Pa. Axisymmetric body of revolution is arranged inside the said vessel, on its bottom and aligned with it, the body height being equal to or exceeding the level of the apparatus maximum filling. Side surface of aforesaid body of revolution smoothly mates the vessel bottom surface. Aforesaid body of revolution represents a cone or a cylinder with aerodynamic nose fairing arranged on its upper end face.EFFECT: higher efficiency of cultivation due to more regular distribution of cless culture in fluid substrate.2 cl, 4 dwg

Apparatus to grow microorganisms // 2352626
FIELD: mechanics, microbiology.SUBSTANCE: apparatus comprises a vessel to receive culture liquid till a certain level. The said vessel has branch pipes to feed liquid mineral culture medium, air and to discharge accumulated biomasses, and, at least, one additional vessel making an adsorber of gaseous substrate. The aforesaid vessels have flanged rings fitted along the vessel axis to divide the vessel part filled with liquid into upward and downward channels. Air feed bubblers are arranged in the vessel upward channels. The vessels lower parts communicate via a liquid duct so that the main vessel downward channel communicates with the additional vessel upward channel, while the additional vessel downward channel communicates with the main vessel upward channel. Note that the liquid duct accommodates a liquid circulation agitator arranged between the vessels. The additional vessel ring upper flange can represent a funnel directed inside the downward channel and symmetrical to the downward channel axis. A surge pipe can be mounted at the flange center to communicate the vessel above-liquid part with the downward channel inner side. Note that another funnel can be mounted below and coaxially with the aforesaid funnel and surge pipe.EFFECT: producing protein masses of aerobic microorganisms in using gaseous substrate as nutrition.4 cl, 2 dwg

ethod and device for making aqueous solution of acrylamide using biocatalyst // 2347814
FIELD: chemistry.SUBSTANCE: proposed aqueous solution of acrylamide is obtained through hydration of acrylonitrile in an aqueous solution in the presence of a biocatalyst. To carry out the reaction, a device is used, comprising a reactor and a centrifuge attached to it. To separate the biocatalyst from the aqueous solution of acrylamide, the centrifuge operates in a semi-continuous mode, at least. The centrifuge has optical means of controlling parameters of the clarified liquid medium tapped from it. The control results are used for controlling operation of the centrifuge. The method has the highest possible environmental friendliness and allows for minimising quantity of by-products.EFFECT: design of an efficient method of making an aqueous solution of acrylamide.16 cl, 1 dwg

ethod of producing aqueous solution of acrylamide utilizing biocatalyst // 2317976
FIELD: industrial organic synthesis.SUBSTANCE: method consists in hydration of acrylonitrile in aqueous solution in presence of biocatalyst, which involves mixing reaction mixture containing acrylonitrile, biocatalyst, and water in reactor provided with closed circulation circuit through which a part of reaction mixture is pumped and wherein at least one heat-exchanger is incorporated. Acrylonitrile is continuously supplied to circulating stream in a point disposed upstream to the point from which reaction mixture is returned to reactor. In this case, concentration of acrylonitrile and/or acrylamide in circulating stream is measured in a point upstream to the point, in which acrylonitrile is added to circulating stream. As biocatalyst, strain Rhodococcus rhodochrous is used. Invention also relates to apparatus for production of acrylamide aqueous solution comprising reactor with circulation circuit incorporating at least one heat-exchanger. In circulation circuit, in the section located immediately before the point from which reaction mixture is returned to reactor, there is a point to receive acrylonitrile, while an on-line device designed to measure concentrations of acrylonitrile and/or acrylamide in circulating stream is incorporated in circulation circuit.EFFECT: prolonged lifetime of catalyst, reduced amount of biocatalyst, suppressed formation of by-products, and optimized level of conversion of acrylonitrile into acrylamide.19 cl, 4 dwg, 1 tbl

Stirrer for the process installation // 2312133
FIELD: chemical industry; food industry; microbiological industry; other industries; production of the stirrers for the process installations.SUBSTANCE: the invention is pertaining to the devices intended for gasification of the liquid medium, for example, by the air and may be used in the different branches of industry, including food industry, chemical industry and microbialogical industry. The stirrer for the process installation system contains, at least, one gas-liquid jet-type mixer with at least one fitting pipe for the gaseous medium and is kinematically connected with the drive gear. At that the fitting pipe of the gas-liquid mixer for the gaseous medium takes its beginning from the surrounding gaseous medium of the process installation disposed over the surface of the treated liquid medium. The presented stirrer for the process installation has more simple design at the expense of exclusion of the compressed air source.EFFECT: the invention ensures, that stirrer for the process installation has more simple design at the expense of exclusion of the compressed air source.12 dwg

ethod for preparing acrylamide aqueous solution using catalyst // 2289626
FIELD: organic chemistry, chemical technology.SUBSTANCE: invention relates to synthesis of organic compounds. Acrylonitrile is hydrated in aqueous solution in the presence of a biocatalyst. Control for carrying out the reaction is carried out by measurements of parameters of the technological process in "on-line" regimen by infrared spectroscopy method with Fourier-transformation. The reaction is carried out in reactor fitted by circulation contour wherein part of the reaction mixture is pumped in closed cycle. Device comprises infrared spectrometer with Fourier-transformation providing carrying out measurements in "on-line" regimen rapidly and to retain activity of biocatalyst at the practically constant level in the reaction course and to produce larger amount of the more pure product.EFFECT: improved method of synthesis.16 cl, 1 dwg

Apparatus for cultivation of microorganisms // 2245915
FIELD: equipment for cultivation of microorganisms.SUBSTANCE: the invention presents an apparatus for cultivation of microorganisms and is dealt with the equipment for cultivation of microorganisms and may be used in different industries and also in exploratory practice. The apparatus includes a horizontally located tubular fermentative container, devices for aeration and mixings of a culture broth and the heat exchanger. Each device contains a body connected to the fermentative container and a circulation circuit for the aerating gas and the culture broth consisting of pipelines, a gas-liquid mixtures pulsating device, a replaceable nozzle and a gas consumption regulator mounted on one of the pipelines. The fermentative container is made out of a transparent heat-resistant glass. At butts of the fermentative container there are protective screens made out of a perforated hydrophobic material. The invention ensures an efficient mass exchange by a uniform distribution of the aerating gas in the whole volume of the culture broth and also an increase of productivity of the apparatus.EFFECT: the invention ensures an efficient mass exchange in the whole volume of the culture broth and an increase of productivity of the apparatus.3 cl, 3 dwg

ethod for the production of fodder yeast and installation for its implementation // 2219237
The invention relates to biotechnology, in particular hydrolytic microbiological industry

Bioreactor // 2208004
The invention relates to biotechnology and can be used in the enzymatic processing of organic waste vegetable and animal household waste, including waste of timber and woodworking industry, etc

Device for the ejection liquid and the ejection method using this device // 2203128
The invention relates to a device and method of ejection of the liquid inside the vessel containing the liquid above the level of the last or towards the side walls of the container

Apparatus for temperature control suspension // 2157835
The invention relates to nutritional and microbiological industry, in particular the yeast production, and is used for cooling and temperature control of the commodity concentrate yeast

Device for culturing autotrophic microorganisms // 2148635
The invention relates to agriculture and microbiological industry

Installation for cultivation of halophilic microorganisms // 2105058
The invention relates to medical and microbiological industry, and specifically to systems for cultivation of microorganisms

Heat pump installation for energy-saving technologies // 2078808
The invention relates to biotechnology and can be used in the production of proteins, vitamins, enzymes, hydrolytic production, food processing, oil refining and t

Device for culturing microorganisms // 2077570
The invention relates to a device for growing sitoindosides microorganisms and intended for use in microbiological industry

The fermenter // 2076148
The invention relates to the field of Microbiology, in particular to the equipment for cultivation of microorganisms under anaerobic conditions, for example, in the production of therapeutic lactic acid products

Installation for cleaning and deodorization gas emissions fermentors // 2060273
The invention relates to methods of cleaning and deodorization gas emissions (GW) fermentors and can be used in microbiological, enzymatic and hydrolytic industry

Heat and mass transfer apparatus // 2055881
The invention relates to a heat and mass transfer equipment, in particular for microbiological, chemical, food industries, and can be used in the apparatus for growing microorganisms

Device for culturing microorganisms // 2031934
The invention relates to a device for working with microorganisms, namely, units for Kolb, and can be used in biotechnology and Microbiology, for example, by culturing microorganisms and cells of animals and plants in tanks and vessels

ixing device for multiphase media // 2021849
The invention relates to biotechnology and can be used when carrying out the processes of microbiological synthesis, automation of scientific research and the development of new technological processes associated with the cultivation of microorganisms, medical and food industries, as well as in the chemical industry

Apparatus for the cultivation of microorganisms // 2021346
The invention relates to the microbiological industry, and specifically to apparatus for growing microorganisms, and is intended for use in the production of fodder yeast, protein-vitamin concentrates and other products of microbiological synthesis
 
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