Biochemistry and beer and spirits and wine and vinegar and microbiology and enzymology and mutation or genetic engineering (C12)

C   Chemistry; metallurgy(312744)
C12            Biochemistry; beer; spirits; wine; vinegar; microbiology; enzymology; mutation or genetic engineering(33233)
ethod for dielectric microcontainer administration into a mammal cell using femto-picosecond laser pulses // 2614269
FIELD: biotechnology.SUBSTANCE: optical trap for microcontainer is created using a focused laser beam with a wavelength of 830 nm. The microcontainer is brought into contact with the cell membrane by exposing it to laser pulses trains with a wavelength of 780 nm, with subsequent cutting of cell membrane by focused laser pulses and controlled introduction of the microcontainer into the cell using an optical trap.EFFECT: invention allows to deliver the microcontainer with genetic material to the given mammal cell coordinate with high precision.4 cl
ethod for determining toxicity of chemicals generating active oxygen forms // 2614267
FIELD: chemistry.SUBSTANCE: method provides adding riboflavin to the final concentration of 1⋅10-5 mg/ml - 1⋅10-3 mg/ml into the Escherichia coli K12 MG1655 culture with the pColD-lux plasmid, in which the bioluminescence lux operon of marine photobacteria Photobacterium leiognathi, Vibrio fischeri or Photorabdus luminescens is placed under the control of the pColD promoter. Adding the test substance and determining the luminescence intensity of the resulting suspension and the control are carried out. The damaging effect of the test substance is estimated by the suspension luminescence intensity deviation from the control.EFFECT: increasing the induction factor.4 cl, 1 dwg, 2 ex
ethod for preparing dosage for production of sparkling wines // 2614265
FIELD: food industry.SUBSTANCE: in bottling-stable white or red table wine stocks or in champagnised wine sugar at the rate of 700-800 g/dm3 and organic acids: citric, lactic and malic acid in a ratio of 1:1:0.5 are added n an amount providing the achievement of the mass titratable acids concentration in the dosage of 6.0-8.0 g/dm3. The mixture is separated into two parts, one of which is heated to 40°C, and into the other part the sedimentary yeast settling in an acratophore during champagnisation is introduced in an amount of 5-10% by volume of the dosage, and heated to 50°C. Both parts of the dosage are mixed at the ratio of 3:1, maintained for 30 days and filtered.EFFECT: invention allows to reduce the time for the dosage preparation and maintaining process up to 30 days instead of 100 days and to improve its organoleptic and physico-chemical parameters.3 ex

Optimized recombinant protein encoding gene - human interferon beta analog // 2614264
FIELD: biotechnology.SUBSTANCE: nucleotide sequence encoding a recombinant protein - interferon beta analog, optimized for expression in E. coli cells. E. coli BL-30-B cell, intended for interferon beta recombinant protein production is obtained by transformation of E. coli BL (DE3) cells by rET30a plasmid containing the said nucleic acid. E. coli BL-32-B cell, intended for interferon beta recombinant protein production is obtained by transformation of E. coli BL (DE3) cells by rET32a plasmid containing the said nucleic acid.EFFECT: invention provides a recombinant protein.3 cl, 5 dwg, 9 tbl, 9 ex
Trametes hirsuta strain - producer of ethanol // 2614263
FIELD: biotechnology.SUBSTANCE: Trametes hirsuta MT-24.24 basidiomycete strain has the ability to produce ethanol. Trametes hirsuta basidiomycete strain is deposited in the Russian National Collection of Industrial Microorganisms under accession number VKPM F-1288 and can be used in medicine and food industry.EFFECT: invention allows to obtain high quality alcohol containing no impurities such as acetone and isopropanol.1 tbl, 5 ex
Strain of bacteria micrococcus luteus 805 - producer of site-specific methyl-dependedn endonuclease mluvi // 2614262
FIELD: biotechnology.SUBSTANCE: strain Micrococcus luteus, deposited under registration number B-12410 in The All-Russia Collection of Industrial Microorganisms of GosNIIGenetika FSUE is the producer of the site-specific methyl-dependent endonuclease MluVI. The invention provides a new MD-endonuclease enzyme which recognizes and cleaves both strands of the DNA sequence 5'-GCGC^NGCGC-3'/3'-CGCGN^CGCG-5' before the central nucleotide (N) with formation of single-nucleotide 5'-protruding ends in the presence in a given sequence of at least six C5-methylized cytosines and can be used for large-site-specific hydrolysis of DNA containing C5-methyl-cytosine bases.EFFECT: increase the efficiency of strain application.2 dwg, 1 tbl, 3 ex
Two-layer, flat, transparent hydrogel substrate for long-term cell cultivation // 2614260
FIELD: biotechnology.SUBSTANCE: two-layer, flat, transparent hydrogel substrate is proposed for long term cell cultivation and the method to get it. The substrate in the hydrated state has a thickness in the range of 25-500 microns, and the radius of menisci curvature of 10-150 microns. The hydrogel substrate includes a structure forming layer consisting of a three-dimensional collagen hydrogel and an upper protein layer. The three-dimensional hydrogel comprises collagen of type I, collagen of type III and proteins that promote cell adhesion and migration. The upper protein layer contains collagen of type IV, laminin and fibronectin. The method of substrate preparing comprises mixing of solutions of collagen of type I, collagen of type III and proteins that promote cell adhesion and migration and further gelation and vitrification, followed by proteins immobilization on the surface.EFFECT: preservation of phenotype by endothelial cells and epithelial cells in their long-term cultivation.13 cl, 1 dwg, 3 tbl, 8 ex

ethod of producing l-amino acids // 2614258
FIELD: biotechnology.SUBSTANCE: invention relates to biotechnology. Method of producing L-lysine using recombinant coryneform microorganism able to produce L-lysine is disclosed, where recombinant coryneform microorganism is transformed by means of insertion of acetate-inducible promoter below stop codon of target gene on chromosome for attenuation of target gene expression and enhancing of ability of recombinant coryneform microorganism to produce L-lysine, where target gene is gene located in nodal point of L-lysine biosynthesis path.EFFECT: invention increases production of L-lysine in comparison with parent strain of microorganism.4 cl, 2 dwg, 12 tbl, 13 ex
ethod of detecting of cell culture contamination with viruses by immunoperoxidase method // 2614256
FIELD: biochemistry.SUBSTANCE: invention relates to biochemistry. Method of detecting contamination with viruses of cell culture by immunoperoxidase method is described, involving reaction of antigen with labelled antibodies in clean cell culture and accounting of reaction results by color density of formed complex under microscope. Wherein reaction uses tablets with analyzed cell culture, contaminated with diarrhoea virus, and after adding into tablet wells of 0.10–0.12 ml of specific homologous serum diluted by 1:64 – 1:128 it is incubated, washed, then 0.10–0.12 ml of anti-specific immunoenzymometric conjugate is introduced, consisting of peroxidase marked antibodies against globulins of cattle blood serum, it is incubated, washed, substrate mixture is introduced consisting of 5-aminosalicylic acid and hydrogen peroxide, and 30–40 minutes later results are accounted under light microscope by formation of brown color in virus-containing cells. Invention improves control system of cell lines latent contamination with viruses used in laboratory practice and biological industry.EFFECT: invention allows to prevent biological contamination and is extremely important stage in production of vaccines and diagnostic preparations; this method can be used for certification at absence of viruses in again coming cell cultures; invention is practically feasible, cheap method of monitoring of cell cultures for content of virus and may be recommended for extensive use both in scientific research and practical virological laboratories, and in production in making diagnostic preparations and vaccines.1 cl, 2 ex

Automated extraction of nucleic acids by size // 2614255
FIELD: biochemistry.SUBSTANCE: invention relates to biochemistry. Invention is described, which includes method for selection of nucleic acids by size. Method involves stages of movement of nucleic acids from sample via channel by means of electrophoresis, automatic tracking of movement of reference fraction of nucleic acids via channel, assessment of estimated time of arrival of target fraction of nucleic acids in hole for extraction in channel, extraction of fluid medium containing target fraction from hole to extract at estimated time of arrival.EFFECT: invention extends range of products for selection of nucleic molecules by size.44 cl, 15 dwg

Diagnostic markers // 2614254
FIELD: biochemistry.SUBSTANCE: invention relates to biochemistry. Method of determining sensitivity of tumor cell growth to inhibition by EGFR kinase inhibitor is disclosed, which involves determining ERBB2 gene methylation status in tumor cell sample, reduced level of methylation ERBB2 gene means, that less than 50 % of possible methylation sites in part of ERBB2 gene are methylated, and indicates that growth of tumor cells is sensitive to inhibition by inhibitor EGFR. Method of detecting patient with malignant tumor is also described, favorable effect on which can have treatment with help of EGFR inhibitor. Method of selecting therapy for patient with malignant tumor is described. Invention extends range of methods for prediction of response to anticancer therapy.EFFECT: method of determining ERBB2 gene overexpression in cell is disclosed.31 cl, 8 dwg, 6 ex

icroorganisms producing o-acetyl homoserine and method for o-acetyl homoserine production using this microorganism // 2614253
FIELD: biotechnology.SUBSTANCE: this microorganism is characterized by weakened or inactivated endogenous citrate synthase activity. As a result of this modification, the is microorganism capable of producing O-acetyl homoserine with high yield. The invention also relates to a method for O-acetyl homoserine production. This method comprises culturing of the said microorganism and separation of O-acetyl homoserine produced by culturing of this microorganism.EFFECT: invention allows to obtain O-acetyl homoserine with high yield.7 cl, 2 dwg, 10 tbl, 4 ex

Composition for producing hydrogel based on polyvinyl alcohol for immobilization of microorganisms // 2614249
FIELD: chemistry.SUBSTANCE: composition for producing hydrogel based on polyvinyl alcohol for the immobilization of microorganisms is described, consisting of a polyvinyl alcohol and a catalyst of cerium-ammonium nitrate crosslinking, characterized in that the initial components are taken in the molar ratio of EVOH: (NH4)2Ce(NO3)6 (160-110):(1-1.3).EFFECT: ensuring formation of water-insoluble hydrogel for immobilization of microorganisms, improved accuracy and sensitivity of the assay using the biosensor.18 cl, 1 dwg,1 tbl, 1 ex
Yeast transformant schizosaccharomyces pombe, producing lactic acid (versions) method for production thereof (versions), method for lactic acid microbiological synthesis using this transformant // 2614233
FIELD: biotechnology.SUBSTANCE: transformant with gene ldh, encoding lactate dehydrogenase from Lactobacillus acidophilus or an enzyme with amino acid sequence homologous to it by at least 93% are proposed. Also a transformant with gene ldh, encoding lactate dehydrogenase from Lactobacillus acidophilus or an enzyme with amino acid sequence homologous to it by at least 93% are proposed, wherein one or several genes encoding enzymes involved in the ethanol biosynthesis pathway are deleted or inactivated. A transformant with gene ldh, encoding lactate dehydrogenase from Lactobacillus acidophilus or an enzyme with amino acid sequence homologous to it by at least 93% are proposed together with gene ldh encoding lactate dehydrogenase from Lactobacillus plantarum or Lactobacillus pentosus. A transformant with gene ldh, encoding lactate dehydrogenase from Lactobacillus acidophilus or an enzyme with amino acid sequence homologous to it by at least 93% are proposed together with gene ldh encoding lactate dehydrogenase from Lactobacillus plantarum or Lactobacillus pentosus,wherein one or several genes encoding enzymes involved in the ethanol biosynthesis pathway are deleted or inactivated. Also, versions of the preparation method for these transformants and the method for microbial synthesis of lactic acid are proposed.EFFECT: efficient production of lactic acid at low concentrations of by-product.13 cl, 10 dwg, 8 ex

Storage stable liquid detergent or cleaning agent containing protease and cellulase // 2614130
FIELD: biochemistry.SUBSTANCE: invention relates to biochemistry. Application of modified protease as agent for increasing storage stability of cellulase in liquid detergent or cleaning agent, involving cellulase and protease is disclosed.EFFECT: invention provides low deactivation of cellulase by said protease thus allowing to preserve high residual activity in said detergent or cleaning agent even after 8 weeks of storage.8 cl, 2 tbl, 1 ex
ethod for production of recombinant pseudoadenovirus particles concentrate, expressing hemagglutinin gene of influenza a/california/07/2009(h1n1) // 2614127
FIELD: biotechnology.SUBSTANCE: method for production of recombinant pseudoadenovirus particles (RPAN) concentrate, expressing the hemagglutinin gene of influenza a/california/07/2009(H1N1) is proposed. The method comprises: obtaining of a production cell culture; preparation of disaggregated starting RPAN culture samples; single-round infection of the production suspension cell culture, thereby obtaining a RPAN-containing crude suspension, with subsequent cell mass precipitation by centrifugation and spent culture medium diverting; precipitated cell mass resuspension in lysis buffer, then one-time freezing followed by suspension thawing the slurry and RPAN separation from the disrupted cells successively by centrifugation, ultrafiltration, anion exchange chromatography, size exclusion chromatography, sterile filtration to yield a sterile pharmaceutical grade RPAN concentrate with high level ofchromatographic purity.EFFECT: method allows to obtain an increased yield of RPAN and a possibility to obtain a concentrate with high titers and chromatographic purity, that meets the quality of a pharmaceutical product suitable for industrial production of influenza vaccines.1 dwg, 1 tbl, 5 ex

Fungi strain from sordariomycetes class - producer of eremoxylarin a antibiotic // 2614126
FIELD: biotechnology.SUBSTANCE: ascomycetous fungi strain of Sordariomycetes INA 01108 class has the ability to produce eremoxylarin A antibiotic. Ascomycetous fungi strain of Sordariomycetes class is deposited in the Russian National Collection of Microorganisms of the Federal State Budget Institution Science Institute of Biochemistry and Physiology of Microorganisms named after G.K. Skryabin, RAS under accession number VKM F-4676D and can be used in medicine.EFFECT: invention allows to increase the yield of eremoxylarin A antibiotic.4 dwg, 2 tbl, 1 ex

Reduction of level of lactate and increasing of production of polypeptide by inhibiting expression of lactate dehydrogenase and pyruvate dehydrogenase kinase // 2614125
FIELD: biotechnology.SUBSTANCE: invention relates to biotechnology and concerns method of reducing lactate production in mammalian cells, method of silencing or reduction in mammal cell of transcription of lactate dehydrogenase (LDH) and pyruvate dehydrogenase kinase (PDHK), method of producing mammal cells, which exhibits low production of lactate in culture, vector containing first heterologous nucleotide sequence coding small interfering RNA (siRNA), specific for lactate dehydrogenase, and second heterologous nucleotide sequence coding siRNA specific for pyruvate dehydrogenase kinase, each of which is connected with its promoter.EFFECT: presented inventions allow reducing in cultured cells production of lactate and increasing of production of heterologous polypeptide.40 cl, 6 dwg
Optimized gene encoding recombinant ipfiii protein // 2614124
FIELD: biotechnology.SUBSTANCE: nucleotide sequence encoding a recombinant interferon lambda protein is optimized for expression in E. coli cells and cloned into the rET30a plasmid. E. coli BL-30-L cell, intended for interferon lambda recombinant protein production is obtained by transformation of E. coli BL (DE3) cells by rET30a plasmid containing the said nucleic acid. The invention provides a recombinant interferon lambda protein with biological activity of 2.1*109 U/mcmol in MDBK/VSV system.EFFECT: increased biological activity.2 cl, 4 dwg, 3 tbl, 6 ex
ethod for determining chemicals genotoxicity // 2614122
FIELD: chemistry.SUBSTANCE: method provides adding riboflavin to the final concentration of 1⋅10-7 mg/ml - 1⋅10-5 mg/ml into the Escherichia coli K12 MG1655 culture with pColD-lux plasmid, in which the bioluminescence lux operon of marine photobacteria Photobacterium leiognathi, Vibrio fischeri or Photorabdus luminescens is placed under the control of the pColD promoter. Adding the test substance and determining the luminescence intensity of the resulting suspension and the control are carried out. The damaging effect of the test substance is estimated by the suspension luminescence intensity deviation from the control.EFFECT: increasing the induction factor.4 cl, 1 dwg, 2 ex

Event das-40278-9 aad-1, related lines of transgenic maize and their event-specific identification // 2614120
FIELD: biochemistry.SUBSTANCE: invention relates to biochemistry, namely to transgenic maize, which is resistant to herbicides 2,4-D and quizalofop, its seeds and part. Method of detecting transgenic event in maize is also disclosed, characterized by SEQ ID NO: 29, in sample using polynucleotide, which is characterized by nucleotide sequence selected from group consisting of SEQ ID NOs: 1–28 and 30–33, or using set including primers. Invention also relates to polynucleotide, which provides resistance to herbicides 2,4-D and quizalofop, as well as to method of collecting offspring of corn plants containing SEQ ID NO: 29, method detection of offspring of corn plants containing feature of resistance to herbicides 2,4-D and quizalofop.EFFECT: invention makes it possible to efficiently obtain transgenic maize, which is resistant to herbicides 2,4-D and quizalofop.15 cl, 7 dwg, 29 tbl, 12 ex
Application of anabaena sp. pcc 7120 strain for silver nanoparticles production // 2614118
FIELD: biotechnology.SUBSTANCE: during biological recovery of silver with nanoparticles production, incubation is performed under constant illumination of the Anabaena sp. PCC 7120 in nitrogen-free medium with silver nitrate.EFFECT: invention provides a solution of silver nanoparticles, wherein the nanoparticles exist in a free state.
ethod for apaf1 polymorphism determination, associated with holstein cattle hh1 fertility haplotype // 2614117
FIELD: biotechnology.SUBSTANCE: method for polymorphism determination for genes associated with cattle fertility, namely with apoptotic protease-activating factor 1 (APAF1) gene, associated with Holstein cattle HH1 fertility haplotype. The method includes PCR-RFLP determination of CT polymorphism→ at 63150400 position (genome assembly UMD 3.1) of APAF1 gene. At that, amplification of the gene fragment containing a mutation is carried out using two primers. The nucleotide substitution leading to the elimination of non-specific BstC8I endonuclease non-specific restriction site located at a distance of 10 bp "down stream" of the investigated mutation, is introduced into the reverse primer followed by restriction PCR products hydrolysis with BstC8I endonuclease. Shorter fragments resulting from restriction correspond to Q allele, while a longer non-restricted fragment corresponds to X allele. Animal genotypes identification is based on the results of PCR-RFLP products electrophoresis in agarose gel.EFFECT: method can be used in farm animals genetics to detect polymorphism in the cattle APAF1 gene, associated with HH1 fertility haplotype, in order to use the results in population genetics, cattle breeding and selection.2 dwg
ethod of producing probiotic composition // 2614116
FIELD: biotechnology.SUBSTANCE: invention relates to biotechnology, food and medical industry and can be used in production of probiotics for direct consumption or biologically active additive to food products, in production of fermented milk products of preventive purpose, intended particularly for normalization of cholesterol level, and useful microflora of gastrointestinal tract and increasing general body resistance. Invention involves method of producing probiotic composition for lowering of blood cholesterol, containing dry biomass of probiotic bacteria strains B. bifidum GG-72 VKPM Ac-1884, L. fermentum LFM-2 VKPM V-10368, L. rhamnosus LC-52GV VKPM V-9475, L. plantarum GVI-1 VKPM V-8556, L. acidophilus AST-44. Above method involves separate cultivation of strains B. bifidum GG-72 VKPM Ac-1884 on Blaurock medium, strain L. fermentum LFM-2 VKPM V-10368, L. rhamnosus LC-52GV VKPM V-9475, L. plantarum GVI-1 VKPM V-8556 and L. acidophilus ACT-44 B. bifidum GG-72 VKPM Ac-1884, L. fermentum LFM-2 VKPM V-10368, L. rhamnosus LC-52GV VKPM V-9475, L. plantarum GVI-1 VKPM V-8556, L. acidophilus AST-44 VKPM-V-9647 on MRS broth at 37±1 °C up to stationary phase of development, concentration, mixing with protective medium, drying and mixing of dry biomass of probiotic strains in equal quantities.EFFECT: method of producing probiotic composition is disclosed.2 cl, 8 dwg, 6 tbl

Bacteriophages, phage peptides and methods for application thereof // 2614114
FIELD: biotechnology.SUBSTANCE: invention relates to biotechnology and virology. Described is bacteriophage F510/08, comprising genome, which contains nucleic acid sequence SEQ ID NO: 4. Bacteriophage shows activity upon Pseudomonas aeruginosa. Invention also describes versions of pharmaceutical composition containing such bacteriophage, and methods of therapy for treatment and prevention of bacterial infection.EFFECT: presented group of inventions can be applied in medicine.23 cl, 327 dwg, 7 tbl, 7 ex
ethod for point mutation diagnosing in native dna using graphene oxide // 2614111
FIELD: biotechnology.SUBSTANCE: for point mutations (substitutions, insertions, deletions) diagnosis in the native DNA, allele-specific PCR carried out with two fluorescently labeled forward primers complementary to DNA sequences of different alleles near the mutation site, and reverse common primer, with subsequent addition of a graphene oxide suspension (as a selective nanostructured fluorescence quencher) to PCR products and measurement of resulting solution fluorescence intensities in two fluorescence channels corresponding to the forward primers fluorescent labels. For comparison and obtaining of diagnostic results, negative control samples not containing DNA on PCR step are applied. Comparison of fluorescence intensities in the final DNA analyte sample solution in two fluorescence channels with the same intensities for negative control, allows to determine the analyzed DNA sample genotype. At the same time, for 4582insT mutation diagnosis in exon 25 of the CUL7 gene causing 3M syndrome in Yakuts, 5'-FAM-CAGGGGTCCTCAAGATTTCG-3'; 5'-ROX-CAGGGGTCCTCAAGATTCG-3' oligonucleotides are used as forward primers; 5'-GATGAGGCAGTTCAGAAGATTCC-3' oligonucleotide is used as a reverse primer.EFFECT: development of a reliable method for point mutations diagnosis in the native DNA using graphene oxide as a selective fluorescence quencher, promising for development of methods for DNA diagnostics of hereditary and genetically predisposed diseases, pharmacogenetic tests.2 cl, 4 dwg, 3 tbl
Bifidobacterium adolescentis 150 strains and bifidobacterium angulatum gt 102, synthesizing gamma-aminobutyric acid // 2614110
FIELD: biotechnology.SUBSTANCE: invention group relates to B.adolescentis 150 B.angulatum GT 102 bacteria strains. B.adolescentis B.angulatum strains are deposited in the Russian National Collection of Industrial Microorganisms under accession numbers VKPM Ac-1974 and VKPM Ac-1973 and have the ability to synthesise gamma-aminobutyric acid (GABA). These strains can be used to obtain probiotic preparations.EFFECT: invention allows to expand the range of gamma-aminobutyric acid producers.2 dwg, 5 tbl, 6 ex
Bhk-21/13-02-transplantable monolayer-suspension subline of newborn syrian hamster kidney cells, intended for fmd virus and rabies virus reproduction // 2614074
FIELD: biotechnology.SUBSTANCE: invention relates to suspension culturing of BHK-21 cells transplanted crops. BHK-21/13-02 - adapted line to suspension method of cultivation using a nutrient medium for suspension cultivation based on hydrolysates: muscle and lactalbumin. BHK-21/13-02 cell culture is intended for industrial reproduction of FMD virus type A, O, C, Asia, rabies virus of "Shchelkovo-51" strain used for the manufacture of antiviral vaccines against FMD and rabies. BHK-21/13-02 cells are sensitive to the foot and mouth disease virus, accumulating in an amount of 7.01 g TCD50/ml, at immunogenic component content up to 1 mlg/ml and are sensitive to rabies virus, providing the infectivity titer of 6.5LD 50/ml.EFFECT: increased sensitivity of cells.

Insecticidal proteins // 2613778
FIELD: biotechnology.SUBSTANCE: invention relates to constructed insecticidal Cry1Ba protein, active against European corn borer, to the encoding nucleic acid, to the structure comprising the above nucleic acid, as well as insecticidal compositions containing the above protein. A recombinant expression vector, a host cell, a plant and a seed comprising the above structure are also disclosed. The invention also relates to a method for controlling corn borer, comprising corn borer bringing into contact with an effective amount of insecticidal Cry1Ba protein.EFFECT: invention can effectively fight the corn borer.14 cl, 2 dwg, 9 tbl, 10 ex

ethod of detecting mutations in complex dna mixtures // 2613489
FIELD: biochemistry.SUBSTANCE: in some applications non-mutant DNA is present in the sample in thousandfold excess. Method involves molecular bar-coding of DNA molecules, their amplification, sequencing of new generation and analysis of sequence data.EFFECT: present invention is aimed at methods for detection in sample of mutations in DNA, where mutant DNA is present on the background of a non-mutant DNA.3 cl, 5 dwg, 5 tbl, 4 ex
Plankton strain chlorella kessleri, intended for production of biomass // 2613424
FIELD: biotechnology.SUBSTANCE: strain is deposited in the Russian National Collection of Industrial Microorganisms under accession number All Russian Collection of Industrial Microorganisms Al-12 and can be used for production of biomass used for feeding of livestock animals, as well as the algolizant of water bodies in course of their biological rehabilitation and wastewater treatment.EFFECT: plankton strain of unicellular green alga Chlorella kessleri NF has a high productivity.1 dwg, 1 tbl, 1 ex
Bacteriophages production method // 2613423
FIELD: biotechnology.SUBSTANCE: method comprises culturing bacterial cells of host strain in the absence of extraneous microflora, phage lysate preparation and purification by precipitation and/or filtration. After 30-120 minutes after culturing initiation, at the optimum growth temperature for the culture of rapidly growing host strain, every 30-60 minutes, smears are made from the host strain culture from the surface of solid nutrient medium or from a liquid culture medium. The smears are stained with a solution of acridine orange in the final concentration of 0.001% to 0.02% or acridine yellow solution to the final concentration of 0.01% to 0.2%. The stained smear is microscoped in fluorescence microscope. Time is set for inoculating the mother bacteriophage at achievement in the stained smear of not less than 50% in relation to the total host strain cells proportion of orange acridine stained cells, fluorescing with orange or red shades, or the proportion of yellow acridine stained cells fluorescing with yellow or orange shades, at achevement of less than 10% in relation to the total host strain cells proportion of cells with non-uniform fluorescence that is paired pole adjoining cells in the form of rods with irregular or spherical cytoplasm fluorescent and spherical or ellipsoidal cells with slightly fluorescenting central section. Then mother bacteriophage is inoculated.EFFECT: stability of achieving high bacteriophage titer upon phage lysate production in a changing nutritive medium formulations, and increase in the range of variations of the indicator values of the host strain and bacteriophage culturing.11 ex

Antibody for blys // 2613422
FIELD: medicine, pharmacy.SUBSTANCE: invention refers to biochemistry. The antibody for BLyS is stated. The invention also relates to the DNA molecule encoding the said antibody, the expression vector and the host cell to produce the said antibody. It is also proposed to use BLyS antibodies in a pharmaceutical composition and in the method for prevention and/or treatment of diseases caused by excessive proliferation of B-cells, such as systemic lupus erythematosus. The invention allows binding to BLyS with high affinity and inhibition of binding to its receptor with high BR3 specificity.EFFECT: invention adds a new BLyS antibody concept to biochemistry with a proposal to use the antibody for prevention and/or treatment of diseases caused by excessive proliferation of B-cells.15 cl, 10 dwg, 4 tbl, 10 ex

High-affinity human antibodies to cytomegalovirus (cmv) gb protein // 2613421
FIELD: biochemistry.SUBSTANCE: invention relates to biochemistry, namely to monoclonal antibody or its antigen-binding fragment, which specifically binds protein gB of cytomegalovirus (CMV). Invention also discloses nucleic acid coding above antibody, host cell producing above antibody, pharmaceutical composition for treating CMV or induction of resistance to CMV containing said antibody. Invention discloses method of producing above antibody and its antigen-binding fragment, their use for treating CMV or increasing resistance to CMV.EFFECT: invention is capable of specific binding with CMV, which provides effective treatment of diseases associated with CMV protein expression.11 cl, 4 dwg, 2 tbl, 5 ex
Recombinant protein mio-hsp, method of its production, injection preparation for muscle mass increase in farm animals, birds and animals of canids, as well as method of using preparation // 2613420
FIELD: biotechnology.SUBSTANCE: present invention relates to biotechnology and can be used for increasing muscle mass of farm animals, livestock and canines. Fused protein is obtained, consisting of myostatin, Gli-Ser spacer and glucan binding domain of alpha-glucan binding domain of Streptococcus mutans (Mio-HSP), that is used to produce injection preparation for subcutaneous or intramuscular injections in a dose of 50–200 mcg of said protein per one kg of animal body weight or poultry. For producing recombinant protein is grown cell strain E. coli M15 [rMio-HSP] - producer of recombinant protein myocardial HSP, which is obtained by transformation of cells of E. coli M15 plasmid rMio-HSP M15 containing a nucleotide sequence gene coding myocardial HSP, then bind protein myocardial HSP in paragraph 1 in the cell extracts strain E. coli M15 [rMio-HSP] with alpha-glucan binding sorbent by affine interaction incubation procedure, with further washing from unbound bacterial proteins and separating end product.EFFECT: invention allows to effectively induce synthesis of specific antibodies to myostatin, inhibit and, consequently, stimulate growth of muscular tissue.4 cl, 7 tbl, 6 ex
ethod of culture diagnosis of tuberculosis infection // 2613366
FIELD: biotechnology.SUBSTANCE: invention relates to biotechnology. Method involves following. Collected sputum is subjected to fast freezing at temperature from -20 to -25 °C without preserving agents and kept at this temperature from 16 to 24 hours. It is unfrozen at room temperature and treated with 4 % solution of sodium hydroxide containing N-acetyl-L-cysteine during 20-25 minutes. Sodium phosphate buffer 1 M is added and mixture is centrifuged at 3,000–3,100 g for 20–25 minutes. Sample is decanted to separate precipitate. Iron oxide is added to precipitate in final concentration of 0.04 M, while stirring, in sodium phosphate buffer 1 M, pH 6.8 and inoculated on nutrient medium Lowenstein-Jensen.EFFECT: invention allows reducing time for obtaining positive results of dense nutrient medium.1 cl
Strain of methane-oxidizing bacteria methylococcus capsulatus gbs-15 for obtaining of microbial protein mass // 2613365
FIELD: medicine, pharmaceutics.SUBSTANCE: invention refers to the biotechnologies and may be used for obtaining of a microbial protein mass. The strain of the methane-oxidizing bacteria Methylococcus capsulatus GBS-15 having the high growth rate under the conditions of continuous culturing, the resistance to methane homologs in natural gas, and the capability for heterotrophic fixation of carbon dioxide and for the growth at excessive pressure (up to 16 atm) is deposited into the All-Russian Collection of the Industrial Microorganisms under the number All Russian Collection of Industrial Microorganisms B-12549.EFFECT: strain of the methane-oxidizing bacteria methylococcus capsulatus GBS-15 providing the accumulation of biomass up to 32 g/l.3 ex
ethod for early neonatal sepsis diagnosis for newborns on first day of life based on mrna expression profile in buccal scrape cells // 2613297
FIELD: medicine.SUBSTANCE: invention is intended for early neonatal sepsis (ENS) diagnosis for newbornss of the first days of life. In the buccal scrape cells, expression levels of CD68 and IL12A genes are measured relative to the representation of mRNA reference V2M, GUS, TBP or HPRT genes. Based on the obtained expression levels, the canonical linear discriminant function (CLDF) value is calculated by the formula. If CLDF <0.45, a conclusion is made about the absence of infectious and inflammatory diseases. If CLDF> 0.45, ENS is defined.EFFECT: invention provides an effective non-invasive ENS diagnosis.3 tbl, 2 ex
ethod for producing whiskey // 2612917
FIELD: food industry.SUBSTANCE: grain and malt distillate, diluted to 62.5% v, are kept separately in the presence of oxygen at a temperature of 25-30°C for 2-4 months in a battery consisting of 4 enamelled containers loaded with staves in the specific surface amount of 450-500 cm2 per 1 dal of the distillate. The first and third containers are loaded with staves, tempered in cold water, steamed, followed by rinsing with hot and cold water and drying to a moisture of 60% at a temperature of 130-150°C. The first and third containers are loaded with staves, tempered in cold water, processed with an enzyme preparation Bryuzaym BGX in the amount of 1-2 ml per 1 kg of staves during 14 days at a temperature of 26-30°C, followed by rinsing with hot and cold water and drying to a moisture of 60% at a temperature of 130-150°C and charring over an open fire. Distillates are blended on the basis of whiskey derivatization with an alcohol of 40-45 vol%, the blend is subjected to rest, is cold processed and filtered.EFFECT: ready product quality improvement.1 tbl, 3 ex

Isoprenesynthase and gene coding it, method of producing isoprene monomer // 2612916
FIELD: biochemistry.SUBSTANCE: group of inventions relates to biochemistry. Polynucleotide is disclosed, coding protein with isoprenesynthase activity. Protein is disclosed, which has isoprenesynthase activity. Expression vector is also disclosed, containing said polynucleotide or polynucleotide, coding said protein. Host cell for production of isoprenesynthase is disclosed, containing said vector. Method of producing protein with isoprenesynthase activity using above host cell is disclosed. Method of producing isoprene monomer from dimethylallyldiphosphate in presence of said protein or using above host cell in medium with dimethylallyldiphosphate is disclosed. Method of producing isoprene polymer is disclosed involving step of producing isoprene monomer using said method.EFFECT: group of inventions increases production of isoprene by several times in comparison with production of isoprene using isoprenesynthase produced from kudzu pilar or poplar.14 cl, 4 dwg, 33 tbl, 16 ex

System for cell culturing // 2612915
FIELD: biochemistry.SUBSTANCE: group of inventions relates to biochemistry. Cell culturing system, system for assessing intestinal effector agents, containing cell culturing system, are disclosed, methods of cell culturing, production of intestinal organoid and evaluation of treating of intestinal effector agents are also disclosed. Cell culturing system comprises device with liquid channel, liquid source, membrane in channel, intestinal epithelial cells on surface of membrane and bacterial cells adhering to intestinal cells. Method of cells culturing involves providing device with intestinal epithelial cells and bacterial cells, equipment it with culture medium at flow rate sufficient for removal of organic acids and unbound bacterial cells. Method for production of intestinal organoid includes delivery of cultural medium to system and cultivation of epithelial cells of intestine and bacterial cells in vitro. Evaluation method of treating intestine involves contact of intestinal epithelial cells or bacterial cells in system with potential intestinal effector agent and measurement of cell response.EFFECT: invention provides development in vitro model simulating mechanical, structural, absorbent, transport and pathophysiological properties of intestine.41 cl, 23 dwg, 1 tbl
ethod of biotechnological processing of droppings in poultry farming // 2612911
FIELD: biotechnology.SUBSTANCE: invention relates to biotechnology and can be used in processing of fresh chicken droppings. Method involves mixing of bird droppings with moisture-absorbing materials and composting stimulator based on microorganisms and its introduction into substrate. Composting is carried out at temperature of ambient medium and active aeration for 5–7 days. Wherein composting stimulator is consortium of soil microorganisms Trichoderma viridae, Azotobacter chroococcum, Azomonas agilis 1:1:2 with concentration of Azotobacter chroococcum – 2×105 CFU/ml, Azomonas agilis – 4.3×105 CFU/ml, Trichoderma viride – 1.5×104 CFU/ml. As water-absorbing material sawn off hardwood is used in amount, providing specified moisture content of compost mixture, and composting stimulator is introduced into substrate in form of mixture of excrements and sawdust or wood chips in ratio of 1:2.EFFECT: invention makes it possible to restore soil fertility, reducing environmental contamination in area of poultry enterprise.1 cl, 3 tbl

Quick method of cloning and expression of related antibody variable region gene segments // 2612910
FIELD: biotechnology.SUBSTANCE: method antibody production comprising antibody isolation from the eukaryotic cell culture medium consisting of nucleic acid encoding the antibody, wherein the encoding nucleic acid is obtained by amplification of nucleic acids encoding the relative variable domain, using the following as a template in a polymerase chain reaction (PCR): single stranded cDNA derived from RNA of antibody-secreting B cell, and nucleic acids encoding a variable domain, inserted into eukaryotic expression plasmid by ligation independent cloning, where a pool of nucleic acids encoding a variable domain of heavy and light antibody chains is used for insertion, where the B-cell is a single B-cell and where the B-cell and its progeny produce more than 20 ng/ml of antibodies for isolation of nucleic acids segments, encoding antibody variable domains for 7 days of their co-culturing with feeder cells, starting from a single cell; and introduction of the selected nucleic acids segments into eukaryotic expression plasmids is carried out without intermediate isolation and analysis of clonal intermediate plasmids.EFFECT: according to the inventionm the method does not require intermediate cloning, selection and analysis of intermediate plasmids.8 cl, 3 tbl, 4 ex
Vodka production method // 2612908
FIELD: food industry.SUBSTANCE: rectified ethyl alcohol "Alfa" and corrected drinking water are mixed so as to obtain vodka with strength of 40 vol.%. The resulting sorting is thoroughly mixed, sent to filtration through sorption-filtering carbon cells of cartridge type, impregnated with platinum of EPSF.U Pt trademark at filtration speed the next but one unit 250 mm high from 30 to 60 dal/hr. The sorting is cooled to 5-10°C and filtered on coal cleaning battery, consisting of 2 or 3 coal cores at filtration speed to 20.0 dal/h for one core loaded with fresh coal BAU-A, at cooling temperature. Vodka is bottled with a control filtration.EFFECT: improved organoleptic indices of the vodka and its high stability in case of storage.1 tbl, 4 ex

phosph1 peptides and vaccines containing them // 2612905
FIELD: biotechnology.SUBSTANCE: invention relates to biotechnology, namely to anti-tumor vaccines based on epitope peptides MPHOSPH1 and can be used in medicine. Peptide is obtained consisting of amino acid sequence SEQ ID NO: 120. Peptide can contain replacement of C- and/or N-terminal amino acid of above sequence to leucine or methionine. Obtained epitope peptide has ability to induce cytotoxic T-lymphocytes (CTL) in presence of antigen presenting cell (APC) carrying HLA-A*0201 or HLA-A*0206.EFFECT: invention allows inducing immune response against malignant tumor expressing MPHOSPH1 in individual, HLA antigen of which is HLA-A*0201 or HLA-A*0206.14 cl, 6 dwg, 3 tbl, 1 ex

ethod and microfluidic chip for cell culture or cell model // 2612904
FIELD: biotechnology.SUBSTANCE: microfluidic chip and method for cells culturing or cell model in this microfluidic chip are proposed. The microfluidic chip includes microfluidic channels with valves to overlap the channels, a compartment for culturing, a working chamber with a membrane and a means of creating a constant pressure in the closed circuit. At that, the membrane is adapted to change the volume of working chamber. The working chamber, the valves and the compartment inlet are connected by microfluidic channels to form a closed loop of nutrient medium circulation. The working chamber with valves and microfluidic channels is a routing pump. The method comprises filling of microfluidic channels and compartment with nutrient medium, insertion of cells or a cell model into the culturing compartment. At that, constant pressure and pulsing flow of the nutrient medium are created in the circuit.EFFECT: possibility of microcirculation modeling hydrodynamic component in vivo in a microfluidic, as well as organism systemic response simulation.39 cl, 15 dwg

ouse producing binding proteins containing vl-domains // 2612903
FIELD: biotechnology.SUBSTANCE: invention relates to biotechnology and immunology. Mouse for production of immunoglobulin chain is disclosed. Mouse is capable to produce immunoglobulin chain contains in its genome variable light chain domain, fused with constant domain of heavy chain, containing in its germ cells first non-rearranged Vκ-segment of human light chain and non-rearranged human Jκ-segment, functionally connected to endogenous nucleotide sequence of constant mouse heavy chain in endogenous mouse locus of heavy chain. Wherein first non-rearranged Vκ gene segment of human light chain and non-rearranged human Jκ gene segment replace all functional endogenous VH gene segments of mouse heavy chain, all functional endogenous DH gene mouse segments and all functional endogenous JH gene segments of mouse heavy chain in endogenous mouse locus of heavy chain. Use of such mouse for antibody production is also described.EFFECT: production of immunoglobulin chain.22 cl, 21 dwg, 5 tbl, 5 ex

ethods of detecting modification of nucleotides // 2612902
FIELD: biotechnology.SUBSTANCE: group of inventions relates to biotechnology, namely to methods of identifying modified cytosine residues innucleotide sequence. Population of polynucleotides is provided, which contain analyzed nucleotide sequence. First part of above population is oxidized or restored, then it is treated with bisulphite. Polynucleotides are sequenced in first and second parts of population. Residue corresponding to cytosine residue in investigated nucleotide sequence is identifying in first and second nucleotide sequences. In particular version method of identifying 5-methylcytosine in investigated nucleotide sequence is proposed. Population of polynucleotides is oxidized, it is treated with bisulphite, sequenced and residue is identified in treated nucleotide sequence. Presence of cytosine in treated nucleotide sequence indicates presence of 5-methylcytosine in investigated nucleotide sequence. Inventions enable to distinguish residues of 5-methylcytosine, 5-hydroxymethylcytosine and 5-formilcitozin from cytosine at mononucleotide resolution.EFFECT: methods are applicable to all sequencing platforms and can be used in analysis of genomic DNA and/or RNA.27 cl, 5 tbl, 11 dwg

Compositions and methods for diagnosing and treating hyperthyroidism of companion animals // 2612901
FIELD: biotechnology.SUBSTANCE: invention relates to biotechnology, namely to markers of hyperthyroidism, and can be used in medicine for diagnostics of hyperthyroidism in cats. Diagnostic technique for hyperthyroidism in cats contains: (a) determination of level of nucleic acid, coding peptide of sodium/iodine symporter in cat with SEQ ID NO: 2, biological sample of cat; and (b) comparison of nucleic acid level with reference level of specified nucleic acid, determined in control cats without hyperthyroidism, where biological sample comprises tissue, cell or biological fluid, contains DNA or RNA. Wherein high level of above nucleic acid in sample of cat in comparison with reference level is diagnostic for hyperthyroidism in cat.EFFECT: present invention enables more effective diagnosis of hyperthyroidism in cat.8 cl, 2 tbl, 12 ex

H1n1 influenza virus antigens with wide spectrum of activity, optimized using computer tools // 2612900
FIELD: biotechnology.SUBSTANCE: invention relates to biotechnology and virology. Production of optimized HA polypeptides of influenza virus H1N1 is described, causing immune response with wide spectrum of activity in relation to influenza virus H1N1 isolates. Optimized HA polypeptides were developed by series alignments of HA protein sequences and subsequent formation of consensus sequences based on structure of certain viruses H1N1 recovered from 1918 at 2011. Invention also describes composition, fused proteins and VLP containing HA polypeptides. Invention also describes sequence of nucleic acids, subjected to optimization of codons coding HA polypeptides and methods for inducing immune response against influenza virus in subject.EFFECT: disclosed group of inventions can be used in medicine.23 cl, 7 dwg, 3 ex
 
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