Biochemistry and beer and spirits and wine and vinegar and microbiology and enzymology and mutation or genetic engineering (C12)

C   Chemistry; metallurgy(315514)
C12            Biochemistry; beer; spirits; wine; vinegar; microbiology; enzymology; mutation or genetic engineering(33805)

ethods of increasing yield of agricultural crops resistant to 2,4-d // 2628504
FIELD: agriculture.SUBSTANCE: to increase the yield of soybean plants resistant to 2.4-D, they are treated with 2.4-D at the growth stage of V3 and R2 with the application rate from 1000 to 2000 g ke/ha. To increase the yield of a soybean plant resistant to 2.4-D: (a) soybean cells are transformed with the molecule of a nucleic acid comprising a nucleotide sequence encoding aryloxyalkanoate dioxygenase (AAD); (b) transformed cells undergo the selection; (c) soybean plants are regenerated from the transformed cells; and (d) 2.4-Dis applied at the growth stage of V3 and R2 of a 2.4-D-resistant soybean plant which is treated with 2.4-D at the application rate from 1000 to 2000 g ke/ha.EFFECT: invention allows to increase the soybean crop capacity.18 cl, 3 dwg, 44 tbl, 24 ex

icrobial inoculants and fertilisers composition containing them // 2628411
FIELD: biotechnology.SUBSTANCE: microbial inoculant for increasing of plant growth, plant productivity and/or soil quality contains strains of two or more species of bacteria selected from Lactobacillus parafarraginis, Lactobacillus buchneri, Lactobacillus rapi and Lactobacillus zeae. The microbial inoculant contains at least one bacterial strain selected from Lactobacillus parafarraginis Lp18, Lactobacillus buchneri Lb23, Lactobacillus rapi Lr24 and Lactobacillus zeae Lz26. Application of inoculant as a fertiliser. The fertiliser composition contains a microbial inoculant. Application of the fertiliser composition. A method for increasing plant growth and/or productivity. A method to improve the quality of soil. Method for degraded soil or pasture recultivation.EFFECT: inventions make it possible to obtain fertilisers based on microorganisms that are effective in provision of nutrients for plant growth, and are environmentally safe.35 cl, 11 dwg, 5 tbl, 9 ex
icroencapsulated bacterial consortium for gluten degradation in fermented dough and method for obtaining said fermented dough // 2628314
FIELD: food industry.SUBSTANCE: microencapsulated bacterial consortium for gluten degradation contains Lactobacillus plantarum ATCC 8014, Lactobacillus sanfranciscensis ATCC 27652 and Lactobacillus brevis ATCC 14869, encapsulating agents, prebiotics selected from the group including polydextrose, inulin and agave syrup, and trehalose in combination with a proteolytic enzyme of bacterial origin and a proteolytic enzyme of fungal origin commonly used for baking. The method for obtaining a consortium for gluten degradation comprises the stages of separately reacting each strain of lactic acid bacteria, separately cultivating each activated strain in a liquid culture medium until each strain reaches a predetermined concentration, removing for each strain the culture medium excess for concentrating the microorganisms, preferably by centrifugation, with obtaining granules, separately resuspending the granules for each strain in a suspension of the saline solution and adjusting to a predetermined volume, mixing the required amounts of each strain and adjusting to a final volume, dissolving the encapsulating agents in water at suitable proportions of dissolved dry substances from 20 to 30%, adding prebiotic and trehalose, inoculating the mixture of the three strains of lactic acid bacteria with obtaining approximately 1010 CFU of said mixture of three strains of lactic acid bacteria per gram of the encapsulated powdery substance, and spray drying with the inlet temperature of 110 to 160°C and the outlet temperature of 60 to 80°C, at a feed rate of 20 to 50 ml/min. Invention also relates to fermented dough, the method for its obtaining, and sweet baked products obtained from the fermented gluten-free dough.EFFECT: group of inventions allows to obtain a consortium which is stable and quickly activated.17 cl, 4 tbl, 6 ex

Single-chemical nucleic acid molecules for gene expression control // 2628311
FIELD: biotechnology.SUBSTANCE: invention relates to biochemistry, in particular to a single-stranded nucleic acid molecule that inhibits target gene expression. The said molecule in the order from the 5'-end to the 3'-end contains the 5'-end portion Xc, the linker portion Lx, the inner portion Z, the linker portion Ly and the 3'-end portion Yc. At that, the inner region Z consists of the inner 5'-end portion X that is complementary to the 5'-end portion Xc and the inner 3'-end portion Y that is complementary to the 3'-end portion Yc. At that, Z consists of 19-30 bases and contains a sequence that suppresses the target gene expression. Z-(Xc+Yc) from 0 to 4 bases, Xc from 1 to 11 bases and Yc from 1 to 11 bases. This invention also discloses a method, application and composition for target gene expression inhibition, method, use and pharmaceutical composition for treatment of a disease caused by an increase in target gene expression using the above-mentioned nucleic acid molecule and the use of such molecule to induce RNA interference.EFFECT: invention allows application of a single-stranded nucleic acid molecule for patients treatment, since it does not cause a side effect in the form of interferon induction.32 cl, 32 dwg, 7 tbl, 25 ex

ethod for recombinant polypeptide production // 2628310
FIELD: pharmacology.SUBSTANCE: method for antibody production, a method for production of a pharmaceutical preparation containing an antibody obtained by this method, a nucleic acid molecule, application of a vector containing such nucleic acid molecule, and application of a cell where the said nucleic acid molecule or vector is artificially introduced in the method for antibody production. This method for antibody production involves culturing a cell that expresses an antibody-enhancing sequence (APES) that has been artificially inserted into a cell and into which an exogenous DNA encoding the desired antibody was introduced to produce the desired antibody, and wherein APES is a nucleic acid molecule containing a nucleotide sequence that can bind to DNA or mRNA of NfkBia gene derived from a human, mouse, rat, or hamster by base pairing, and can suppress expression of the NfkBia gene.EFFECT: invention allows production of an antibody at a high level with suppression of NfkBia expression.16 cl, 29 dwg, 8 ex

New fucosyltransferases and applications thereof // 2628307
FIELD: biotechnology.SUBSTANCE: bacterial host cell is provided comprising a sequence consisting of a polynucleotide encoding a polypeptide with alpha-1,2-fucosyltransferase activity (SEQ ID NO: 1) to produce 2'-fucosyllactose. Also methods for 2'-fucosyllactose production using the said polypeptide with alpha-1,2-fucosyltransferase activity, a donor substrate containing a fucose residue and an acceptor substrate comprising or consisting of lactose.EFFECT: group of inventions allows to facilitate production of fucosylated oligosaccharides of human milk, making it more efficient and economical due to the possibility of lactose application as a substrate for alpha-1,2-fucosyltransferase.9 cl, 13 dwg, 3 tbl, 1 ex

ethod and intermediate compounds for pregabaline production // 2628298
FIELD: pharmacology.SUBSTANCE: invention relates to a method for preparation of a compound of the formula and intermediates used to implement the method, as well as a method for pregabalin preparation.EFFECT: method can be implemented on a commercial scale using readily available, safe raw materials and reagents, and without the need for difficult separations.20 cl, 8 tbl, 41 ex

Insect resistant and herbicide tolerant soybean event 9582.814.19.1 // 2628099
FIELD: biotechnology.SUBSTANCE: selected nucleic sequence, which is the primer. A soybean plant that is resistant to Pseudoplusia includens (soybean moth), a seed for growing of the said soybean plant and a portion of a soybean plant that is resistant to Pseudoplusia includens (soybean moth) are disclosed.EFFECT: invention can effectively fight with lepidopteran insects.7 cl, 3 dwg, 8 tbl, 7 ex
ethod for assessment of cholera vibrio biofilms sensitivity to antibacterial drugs // 2628098
FIELD: biotechnology.SUBSTANCE: method is proposed for assessment of cholera vibrio biofilms sensitivity to antibacterial drugs, including biofilm production on glass cylinders. Cylinders with the resulting biofilm are placed on agar in a Petri dish, antibacterial drug solutions at different concentrations are added in the middle of the cylinder. The results are taken into account after 18-20 h of incubation at 37°C by the presence or absence of cholera vibrios growth in the cylinders prints. Cholera vibrios biofilm sensitivity to antibacterial drugs is assessed by the activity index calculated by the formula: where AI is the antibacterial drug activity index; MICs is the maximum MIC of this antibacterial drug for sensitive strains in accordance with MUK 4.2.2495-09; MICb is MIC value of this antibacterial drug with respect to the biofilm of the strain under study. Biofilm sensitivity of the strain under study is directly proportional to the AI index.EFFECT: accuracy of evaluation of cholera vibrio strains biofilm sensitivity to an antibacterial drug.2 dwg, 4 tbl, 2 ex

Recombinant cell for the production of phenolic compounds and process of producing phenolic compounds // 2628097
FIELD: chemistry.SUBSTANCE: proposed modified recombinant cell-host for the production of phenolic compounds and process for producing phenolic compounds (versions). Host cell contains three expression systems. The first expression contains at least one nucleotide sequence, coding aromatic polyketide synthase (PKS) that contains Synthase of 6-methylsalicylic acid (6-SMSA) or synthesis of orsellic acid (SOA), which may be expressed. The second system expression contains at least one nucleotide sequence encoding a holo-acyl-protein synthetases (APS), wherein APS synthase contains a pantothenic acid of specified PKS. The third expression system comprises at least one nucleotide sequence encoding a functional decarboxylase that catalyses decarboxylation for said production of a phenolic compound such that the product isolated from mentioned cell is a phenolic compound. In this case, either the first expression system of PKS and the second system of expression of APS are present on one vector or on separate vectors and the third functional decarboxylase expression system is either present on the same vector as the first expression system of PKS and/or the second expression system of APS or is present on a single vector, or the first, second or third expression system is integrated into the chromosome of the host cell or is expressed from artificial yeast chromosomes (AYC). The process for producing a phenolic compound involves providing a recombinant host cell containing three expression systems, preparing an intermediate phenolic compound in a recombinant host cell, and synthesizing a phenolic compound from an intermediate phenolic compound. Option method of production of phenolic compounds provide recombinant host cell containing the first and the second expression system, isolating the intermediate phenol compounds produced in recombinant cell-host, and decarboxylation of an intermediate compound for phenol formation of phenol compounds by heating with a metal catalyst with subsequent distillation.EFFECT: obtaining phenol compounds with high purity and output.17 cl, 5 dwg, 1 tbl, 7 ex

Cats calicivirus ashley virus strain for study of drug antiviral activity related to cats calicivirus // 2628096
FIELD: biotechnology.SUBSTANCE: ashley strain off cats calicivirus, deposited in FBIS SSC VB Vector under the number V-697 is obtain, having stable infectious activity, adapted to the transplanted cell cultures.EFFECT: strain can be used to study the antiviral activity of drugs against cats calicivirus.6 dwg, 4 ex

Rsv-specific binding molecules and means for their obtaining // 2628095
FIELD: biotechnology.SUBSTANCE: isolated antibody or a functional part thereof, capable of specifically binding the respiratory syncytial virus (RSV), is proposed. A nucleic acid molecule encoding the antibody is also provided. An expression vector and isolated cells comprising the said nucleic acid molecule for production of said antibody are described. In addition, the invention relates to a method for production of the said antibody, as well as to application of the said antibody in methods for RSV-related disorder treatment or prevention.EFFECT: invention allows to effectively bind RSV and can be used for RSV-related disorders therapy.27 cl, 16 dwg, 2 tbl, 5 ex
ethod for obtaining of msc-associated non-differentiated hemopoietic precursor cells with cd34+/cd133+ phenotype // 2628092
FIELD: biotechnology.SUBSTANCE: invention relates to production of cell cultures enriched with hematopoietic precursor cells with CD34+/CD133+ phenotype. The method includes preparation of a stromal sublayer, addition of an umbilical cord fraction, cultivation and selection. Multipotent stromal cells of adipose tissue (MSC) are obtained from the stromal-vascular fraction of adipose tissue, which are cultured at an O2 concentration of 5% in the environment. Mononuclear fraction of umbilical cord blood (MNF UB) is added to the MSC monolayer. After cocultivation, the undifferentiated hematopoietic precursors are selected from the mononuclear fraction of UB by adhesion to MSC at 20% O2, MSC is continued to be cultured with attached MNF UB for 96 hours at O2 concentration of 20% in the environment.EFFECT: invention allows to obtain a population enriched with undifferentiated hematopoietic umbilical cord blood precursors with this complex phenotype.9 dwg, 1 tbl, 1 ex
ethod for morphogenesis stimulation in barley tissue culture // 2628091
FIELD: biotechnology.SUBSTANCE: invention is a method for morphogenesis stimulation in a barley tissue culture, comprising callus obtaining on a dense Murasige-Skoog medium containing 2 mg/l of dichlorophenoxyacetic acid; passage for proliferation to the medium with 1 mg/l of dichlorophenoxyacetic acid, and three weeks later - for morphogenesis induction - passaging to the medium with 1 mg/l of kinetin, 0.5 mg/l of indolylacetic acid, 0.1 mg/l of gibberellic acid. One medium (for callus proliferation or morphogenesis induction) is prepared not dense, but semi-liquid (4 g/l of agar) with addition of 5 vol % of perfluorodecalin; callus cultivation in a semi-fluid medium at constant shaking (125 rpm) for 2-3 weeks prior to callus appearance and subsequent regeneration of plants on a dense medium.EFFECT: invention allows to increase the yield of barley regeneration plants by stimulating the laying of meristematic zones in callus tissue and increasing the morphogenetic potential in callus lines with reduced regenerative capacity.1 dwg, 3 tbl, 3 ex

ethod for the preparation of surfactant peptides // 2628088
FIELD: biotechnology.SUBSTANCE: expression cassette is constructed containing the sequence encoding the SP-C33(Leu) peptide coding a linker with a protease cleavage site and encoding MBP protein, at that, the said sequences are sequentially fused to a polynucleotide and the polynucleotide is operably linked to a promoter sequence suitable for expression in a prokaryotic cell. The expression cassette is used to prepare an expression vector that is introduced into E. coli cells, cultured under conditions allowing fusion protein expression, purification of the aid protein, followed by fusion protein cleavage with an appropriate protease, and isolation of the SP-C33(Leu) peptide.EFFECT: increased efficiency of recombinant peptide production.13 cl, 3 dwg, 1 tbl
ethod for producing low-molecular chitosan and chitosan oligomers // 2627870
FIELD: chemistry.SUBSTANCE: method for producing low-molecular chitosan and chitosan oligomers by a chemical depolymerization method, including hydrolysis of chitosan in the presence of an acid, followed by filtration, fractionation, purification and drying of products, characterized in the fact that hydrolysis of chitosan is carried out with 2.5-12.5% dilute nitric acid at a temperature of 70°C, followed by separation of the hydrolyzate into two fractions - a precipitate of low-molecular chitosan and a mother liquor, then chitosan oligomers are precipitated from the mother liquor during addition of isopropyl alcohol and cooling. Then, both products are washed with isopropyl alcohol and air-dried, finally, the products of chitosan depolymerization are redissolved in water and dried lyophilically.EFFECT: method is proposed for producing a product of a narrow fractional composition which is highly soluble in water.5 ex

ethod for noninvasive prenatal diagnostics of fetal aneuploidy // 2627673
FIELD: medicine.SUBSTANCE: method for non-invasive prenatal diagnosis of fetal aneuploidy is proposed, which includes extraction of extracellular DNA (ecDNA) from a blood sample of a pregnant woman, selection of genome regions for amplification, preparation of genomic libraries, mapping of the resulting sequences to a reference genome or part of the human genome with coordinates determination, determination of the coverage value for each genome region, characterized by the openness of chromatin between the placenta and mother's blood cells, differing not less than 20%, and obtaining genome regions with the indicated openness of chromatin, after which a conclusion is made about the presence of fetal aneuploidy. A method is proposed to obtain genome regions for carrying out the above noninvasive prenatal diagnosis of fetal aneuploidy.EFFECT: simple and economical way of prenatal diagnosis of fetal aneuploidy in the early stages of pregnancy.7 cl, 3 dwg, 3 tbl, 5 ex
Polybacterial drug with advantages for health: with antioxidant effect, decreased cholesterol concentration, anti-inflammatory and immuno-modulating effect, and release of bioactive peptides inhibiting angiotensin-converting enzyme // 2627651
FIELD: biotechnology.SUBSTANCE: group of inventions refers to polybacterial probiotic drug including new strains of lactic acid bacteria Lactobacillus gasseri 7/12 NBIMCC No. 8720, Lactobacillus plantarum F12 NBIMCC No. 8722 and Lactobacillus helveticus A1, NBIMCC No. 8721, which has anti-inflammatory immunomodulatory, hypocholesterolemic, antioxidant and antihypertensive activity. The group of inventions also refers to the use of drug as a probiotic agent, as well as an agent which has anti-inflammatory immunomodulatory, hypocholesterolemic, antioxidant and antihypertensive activity.EFFECT: food additives, food and functional products, pharmaceutical preparations that include this drug, have a beneficial effect on the health of people and animals.9 cl, 8 dwg, 15 tbl
ethod for prediction of risk of subarachnoidal bleeding due to brain vessels aneurism rupture in persons of asian race // 2627643
FIELD: medicine.SUBSTANCE: DNA is isolated from the peripheral venous blood lymphocytes of the examinee, followed by determination of the A1166C polymorphism genotype of AGTR1 gene (rs 5186). Prediction of an increased risk of subarachnoid bleeding due to cerebral aneurism rupture in persons of the Asian race is accomplished by genomic typing of A1166C polymorphism of AGTRl gene (rs 5186). In the presence of the C allele and AC genotype of A1166S polymorphism of the AGTR1 gene (rs 5186), increased risk of aSAC is predicted.EFFECT: use of the method improves the accuracy of prediction of the risk of cerebral vessels aneurism development and rupture, leading to subarachnoid bleeding in persons of the Asian race.7 tbl, 3 ex
Bacillus megaterium 2-06-ts1 microorganisms strain as photosynthesis and plant growth energy activator // 2627608
FIELD: biotechnology.SUBSTANCE: Bacillus megaterium 2-06-TS1 bacterial strain is deposited in the Russian National Collection of Industrial Microorganisms FGUPGosNIIGenetika under accession number VKPM B-12402.EFFECT: invention improves the energy of seed germination, plant germination, increases root growth, as well as chlorophyll b, carotenoids and the total amount of photosynthesis pigments in plant seedlings.3 tbl
Identification method for a and b versions of human herpes virus 6 // 2627607
FIELD: biotechnology.SUBSTANCE: invention relates to the clinical laboratory diagnosis of virus infections, and may be used for genotyping herpes virus 6 (HHV-6). The method enables to determine one-nucleotide polymorphisms specific for HHV-6A and HHV-6B in the U67 HHV-6 gene via real-time PCR using primers HHV6F 5'-CGGATACAGTAAGACGGGATAT-3' and HHV6R 5'-ACGTAAGCTTGCACAATGC-3' and two fluorescently labeled probes HHV6AZ F1-GCAATAGATTTGAGAACGCGCGGCAT-Q1 and HHV6BZ F2-GCAATAGATTCGGAAATGCGGCAT-Q2, where the presence of HHV-6A or HHV-6B is indicated when the exponential accumulation of the fluorescence signal is registered in the respective probes channels. The invention may be used for genotyping herpes virus 6 (HHV-6).EFFECT: rapid and reliable identification of viruses HHV-6A and HHV-6B.2 dwg, 1 ex
ethod for lipoaspirate processing // 2627606
FIELD: biotechnology.SUBSTANCE: method for lipoaspirate processing is proposed. The method comprises addition of 0.25% ketotifen solution at a dose of 1 ml of solution per 20 ml of lipoaspirate prior to the lipoaspirate washing step.EFFECT: increased adipose tissue cellular fraction viability.1 ex
ethod for producing alcohol-free beer beverage // 2627600
FIELD: food industry.SUBSTANCE: wort is obtained, fermented, subjected to after-fermentation, and ripening is carried out. The beer beverage is clarified and held for 24 hours at the temperature of 4°C. During the holding process, carbon dioxide is removed. Dealcoholization is carried out by the thermal method to alcohol content in the beer beverage of 0% by volume, and, at the end of dealcoholization, aromatic substances are returned and bottling is performed.EFFECT: invention allows to obtain a beverage preserving most of the organoleptic characteristics of the initial product.3 cl, 2 ex
ethod for water surface purification from oil pollution // 2627598
FIELD: biotechnology.SUBSTANCE: method comprises microbial preparation application to a water body; the preparation is based on a consortium of Acinetobacter sp. IB DT 5.1/1 and Ochrobactrum sp. IB DT 5.3/2 microorganisms with a titer of at least 108 CFU/ml in the culture fluid. The purification process is carried out by water body aeration.EFFECT: invention allows to reduce time and improve the efficiency of water bodies purification from oil pollution.2 ex

ammalian cells expressing cd40l ligand and their application // 2627597
FIELD: biotechnology.SUBSTANCE: co-culturing of a pool of rabbit B cells with BHK or CHO cells expressing CD40L, which are used as a feeder in the presence of IL-2 at a concentration of about 50 U/ml and IL-21 at a concentration of approx. 10 ng/ml to approx. 100 ng/ml.EFFECT: invention allows to obtain high concentrations of IgG in a short time.7 cl, 14 dwg, 16 tbl, 16 ex

ethod and structure for synthetic two-directional vegetable promoter scbv // 2627595
FIELD: biotechnology.SUBSTANCE: invention relates to synthetic construct double-stranded nucleic acid for expressing a plurality of heterologous genes in a plant cell, characterized by a bidirectional promoter consisting of a first circuit containing element minimal core promoter, and a second chain comprising a promoter bacillary virus sugar cane, as well as to a method of producing transgenic plant cells or tissues that express a plurality of heterologous genes with its use. The invention also relates to a plant cell, transgenic plant tissue and transgenic plants which express a plurality of heterologous genes and comprise mentioned bidirectional promoter.EFFECT: invention can effectively express a plurality of heterologous genes in a plant cell.29 cl, 56 dwg, 12 tbl, 19 ex
Underground reactor system // 2627594
FIELD: power industry.SUBSTANCE: method for fuel producing from an organic material in an underground reactor (versions) and an underground reactor for use in the above process (versions) is proposed. The underground reactor includes the first conduit for organic material injecting under the ground and converting it into fuel, the second bypass for raising the converted organic material, and a heat exchanger for generating heat to power the equipment where the heat transfer fluid contains piezo-thermal or piezoelectric particles. In another version the underground reactor also comprises a pump for holding the reaction zone at the desired temperature. The method includes sending the organic material under the ground through the first conduit, applying to an organic material in a pressure and temperature reaction zone for converting the organic material into fuel, raising fuel through the second pipeline, and circulating the heat transfer fluid. In another version the method also includes using a heat exchanger to generate heat for use in the equipment powering.EFFECT: obtaining fuel due to underground temperature and pressure.95 cl, 23 dwg, 5 tbl, 13 ex
ethod for c-kit gene l576p mutation diagnostic in melanoma tissue // 2627440
FIELD: biotechnology.SUBSTANCE: invention is intended to diagnose the presence of c-KIT gene L576P mutation in melanoma tissues. Estimation of the presence of mutation is carried out according to the ratios of the values of Ct threshold cycles in three real-time PCR reactions with allele-specific primers. Based on the results of three duplicate reactions, the mutant DNA proportion in the sample is calculated.EFFECT: invention provides optimisation of real-time allele-specific PCR for diagnosis of c-KIT L576P tyrosine kinase receptor mutation in melanoma tissues.6 dwg, 1 tbl, 4 ex

Exotoxin a pseudomonas with less immunogenic t-and/or b-cell epithopes // 2627216
FIELD: biotechnology.SUBSTANCE: A Pseudomonas (PE) ectotoxin is proposed with substitution of one or more amino acid residues at L423, F443 and L477 positions. Also a chimeric molecule containing a targeting agent fused to PE and a molecule containing a targeting agent conjugated to PE. In addition, nucleic acids encoding PE and chimeric molecule; a recombinant expression vector; a host cell for PE production; a pharmaceutical composition for cell growth inhibition; a method for cancer treatment or prevention in a mammal; a method for target cell growth inhibition; methods for production of PE, chimeric molecule containing a targeting agent fused to PE, and molecules where PE is covalently bound to the targeting agent are described.EFFECT: A Pseudomonas exotoxin of this invention exhibits reduced immunogenicity while maintaining cytotoxicity.23 cl, 12 dwg, 20 tbl, 16 ex

Recombinant target toxin specific to her2 receptor expressing cells // 2627215
FIELD: biotechnology.SUBSTANCE: recombinant targeted toxin, specific for HER2 receptor expressing cells is proposed. The recombinant target toxin comprises a HER2-specific targeting module represented by the HER2-specific darpin DARPin class coding sequence and a toxic module in the form of a fragment of pseudomonade exotoxin A ETA interconnected by a flexible hydrophilic 16-amino acid linker represented by the sequence of SEQ ID NO: 1, oligogistidine sequence His6 and KDEL sequence at the C-terminus of the molecule. The recombinant target toxin is represented by the sequence of SEQ ID NO: 2.EFFECT: increased toxic effect on the HER2 receptor expressing cells and increased effectiveness of targeted therapy.2 cl, 6 dwg
ethod for molecular-genetic intraspecific typing of toxygenic vibrio cholerae o1 eltor strains // 2627193
FIELD: biotechnology.SUBSTANCE: method for molecular-genetic intraspecific typing of toxigenic Vibrio cholerae O1 Eltor strains is claimed. The method involves isolation of DNA from the test Vibrio cholerae O1 Eltor strain, PCR conduction with the specific primers, and taking into account the results obtained. Amplification of the test DNA is performed by the designed primers to each of the ICE elements of Indian and Mozambican types, in the presence of intercalating Siber Green dye. The amplification products are then subjected to melting, the results are taken into account by temperature melting peaks and the amplicon size. At the melting point of 87°C and the amplicon size of 300 bp, presence of ICE of Indian type is detected. At the melting point of 79°C and the amplicon size of 149 bp, presence of ICE of Mozambican type is detected.EFFECT: invention allows to quickly and reliably determine the origin of isolated toxigenic of V cholerae O1 Eltor strains.1 dwg, 4 tbl, 3 ex
Transgenal aspen plant, sustainable to herbicides // 2627189
FIELD: biotechnology.SUBSTANCE: invention relates to a transgenic aspen plant having a resistance to phosphinothricin as compared to a non-transformed plant and not having somaclonal changes. This plant contains the BAR gene encoding the phosphinothricin-acetyltransferase enzyme and obtained by agrobacterial transfer of the said BAR gene to aspen Pt.EFFECT: invention makes it possible to efficiently obtain an aspen plant resistant to phosphotrichin.3 cl, 3 dwg, 2 tbl
Vaccine influenza virus a/17/south africa/2013/01 (h1n1)pdm09 for production of live intranasal influenza vaccine for adults and children // 2627188
FIELD: biotechnology.SUBSTANCE: submitted strain A/17/South Africa/2013/01 (H1N1)pdm09 is a reassortant obtained by crossing of wild virus A/South Africa/3626/2013 (H1N1)pdm09 with virus A/Leningrad/134/17/57 (H2N2) - donor of attenuation, harmless to humans. The strain of live influenza A/17/South Africa/2013/01 (H1N1)pdm09 is characterised by a set of symptoms: antigenic specificity of the epidemic virus A/17/South Africa/3626/2013 (H1N1)pdm09, temperature sensitivity and cold adaptation, harmless to laboratory animals, which correlates with human attenuation. The reassortant has inherited the genes coding surface virus antigens - hemagglutinin (HA) and neuraminidase (NA) from the epidemic parent virus, and six genes coding internal non-glycosylated proteins from the attenuation donor.EFFECT: strain can be used in practical health care to prevent the incidence of influenza in adults and children.5 tbl

Bacillus pumilus strain and method for obtaining amikumacin a antibiotic with its application // 2627187
FIELD: biotechnology.SUBSTANCE: method for amikumacin obtaining involves cultivation of a Bacillus pumilus bacteria strain VKPM B-12548 on a liquid nutrient medium containing sucrose, glucose, yeast extract, K2HPO4, NaCl, MgSO4, (NH4)2SO4, FeSO4×7H2O, MnCl2×4H2O, CaCO3 and tap water at a predetermined ratio of components, followed by cultivation for 40-42 hours under aerating conditions. Cells from the culture liquid are precipitated by centrifugation to obtain a supernatant. Amberlite XAD-2 sorbent is added to the supernatant, followed by elution of amikumacin A with n-butanol-acetone-water system at a ratio of 1:1:1.EFFECT: invention allows to increase the yield of amikumacin A antibiotic.2 cl, 3 dwg, 1 tbl, 1 ex

New binding molecules with anti-tumour activity // 2627185
FIELD: biotechnology.SUBSTANCE: binding molecule specifically binding to two different epitopes on HER2 and a method for its preparation, as well as a nucleic acid encoding it, expressing a vector comprising this nucleic acid molecule, a host cell for expression of a binding molecule transformed with the expression vector, and a pharmaceutical composition for cancer treatment comprising a binding molecule or a nucleic acid molecule encoding it, or an expression vector, are proposed. The proposed binding molecule comprises the first polypeptide comprising an amino acid sequence that is at least 90% identical to SEQ ID NO:1, and the second heavy and light chain antibody polypeptide comprising amino acid sequences at least 95% identical to SEQ ID NO:NO:154 and SEQ ID NO:155, respectively. The presented group of inventions is applicable in medicine for anti-tumour therapy.EFFECT: binding molecule effectively suppresses the tumour cells growth and shows high antiproliferative activity.17 cl, 13 dwg, 1 tbl, 14 ex

Aneurinibacillus migulanus strain and its application for gramicidin s production // 2627182
FIELD: biotechnology.SUBSTANCE: invention can be used to produce gramicidin S using the strain of the Aneurinibacillus migulanus VKPMV-10212 producer.EFFECT: resulting strain allows production of gramicidin S in high yield.2 cl, 2 dwg, 2 ex
Expressional plasmid lentiviral vector for heterological expression of recombinant human protein cd47 // 2627181
FIELD: biotechnology.SUBSTANCE: invention relates to a plasmid lentiviral vector for heterologous expression of a recombinant human CD47 protein in mammalian cells. The vector contains a highly functional cytomegalovirus promoter (CMV), a CD47 receptor extracellular domain sequence, a TEV cysteine protease recognition sequence, a tagRFP fluorescent protein sequence acting as a selectable marker. All three sequences are in the same reading frame under the control of this promoter.EFFECT: invention allows to obtain a highly productive and stable protein expression system for the further production of its purified form.2 cl, 1 ex, 4 dwg
Test system for determination of interferon, il23 interleukine and mxa anti-virus protein rna // 2627179
FIELD: biotechnology.SUBSTANCE: test system for multiplex quantitative analysis of expression of interferon lambda, MxA antiviral protein and interleukin 23 genes based on reverse transcription combined with real-time polymerase chain reaction, including specific primers and their corresponding fluorescent hydrolysable probes, as well as negative and positive control samples.EFFECT: invention allows detection, identification and quantification of transcription levels of these genes RNA with high sensitivity, specificity and possibility of multiplex analysis, allows to reduce the cost of analysis by reducing labor, consumption of reagents and plastic and speed up the procedure; can be successfully applied in the control of treatment and prediction the course of human infectious, allergic and autoimmune diseases.2 tbl, 3 ex
ethod for bacillus thuringiensis strains genetic certification by means of multiplex express analysis of reference dna sequences // 2627178
FIELD: biotechnology.SUBSTANCE: invention provides a method for bacillus thuringiensis strains genetic passportization by means of multiplex express analysis of reference DNA sequences. Bacterial DNA is isolated, multilocus PCR is performed to prepare DNA reference complexes using primers containing the sequences of SEQ ID NO: 1-60 and combinations thereof, pyrosequencing the resulting DNA reference complexes to obtain unique genetic certificates of Bacillus thuringiensis strains sufficient to separate strains within one biovar.EFFECT: invention is a high-performance express method that allows determination of Bacillus thuringiensis bacterium specific strains within a single biovar, its application leads to a reduction in the analysis cost and a reduction in its implementation timing.13 cl, 1 tbl, 1 ex
ethod for defibrotide biological activity determination, based on euglobulin application // 2627177
FIELD: biotechnology.SUBSTANCE: defibrotide, mammal euglobulin and a substrate specific for the plasmin which, by reaction with the plasmin, gives a measurable product, are brought into contact. The substrate specific for plasmin is a compound of formula A1-A2-A3-X, where A1 and A2 are non-polar amino acids, A3 is lysine or arginine, and X is the measurable product. The measurable product is selected from the group consisting of para-nitroaniline and 2-naphthylamine. The amount of obtained product is measured at successive time points to determine defibrotide biological activity. The invention provides accurate and precise measurement of defibrotide activity even in the presence of impurities such as, for example, RNA, heparin, degraded defibrotide.EFFECT: sensitive method to detect defibrotide concentrations, and provide good correlation up to the maximum concentration values.14 cl, 3 dwg, 4 tbl

New modulators and methods of their application // 2627176
FIELD: biotechnology.SUBSTANCE: antibody specifically binding to PTK7 (human protein tyrosine kinase 7), as well as a conjugate of the said antibody with a cytotoxic agent, is claimed. Nucleic acids encoding the antibody are also provided and include expression vectors and host cells. In addition, methods for application of the said antibody, including conjugate, for cancer diagnosis and treatment are provided.EFFECT: effective targeting of tumour stem cells, which can be used to treat patients suffering from a wide range of malignant tumours.52 cl, 42 dwg, 5 tbl, 16 ex

Novel peptide with 5 linked ctl epitopes // 2627175
FIELD: biotechnology.SUBSTANCE: peptide made from 5 CTL epitopes of cancer antigens linked through amino acid linkers is obtained. The resulting peptide is used to stimulate peripheral blood lymphocytes in order to obtain a combination of CTL specific for the cancer antigen.EFFECT: invention provides a peptide vaccine for cancer treatment for wide range of cancer patients without the need for HLA typing and regardless of patients' HLA types.6 cl, 2 dwg, 2 tbl, 4 ex

Il-1 alpha and beta bispecific immunoglobulins with double variable domains and their application // 2627171
FIELD: biotechnology.SUBSTANCE: binding proteins, represented by immunoglobulins with double variable domains that bind IL-1α and IL-1β are claimed. In addition, the invention relates to methods for preparation, as well as application of these proteins, including as part of a pharmaceutical composition, for treatment of a disease or disorder in which the IL-1α and IL-1β or IL-1β activity is malicious.EFFECT: invention allows IL-1α binding with IL-1β with high affinity .32 cl, 2 dwg, 24 tbl, 2 ex

Resistant to glyphosate plants and methods related to them // 2627169
FIELD: biotechnology.SUBSTANCE: discloses a method of producing a transgenic plant, plant part, plant organ, plant seed or plant cell which is resistant to glyphosate and method provide resistance to glyphosate in a plant and the use of the aforementioned nucleic acid molecule. The invention also relates to a method of controlling weeds in cultivated crop area comprising herbicide-resistant plant or a herbicide-resistant plant seeds, comprising introducing into the crop area a sufficient amount of glyphosate to control weeds in cultivated crop area without substantially affecting the said seeds or plant.EFFECT: invention helps to efficiently obtain glyphosate tolerant transgenic plant.27 cl, 17 dwg, 9 tbl, 18 ex

Differentiation of human embryonic stem cells // 2627168
FIELD: biotechnology.SUBSTANCE: invention relates to methods for obtaining of a cell population where more than 85% of cells express markers characteristic for the formed endoderm line. This population of cells is obtained by differentiation of the population of human embryonic stem cells of H1 line. Differentiation takes place in a serum-free medium with addition of: (a) BSA, (b) or (i) GDF-8 and 14-prop-2-en-1-yl-3,5,7,14,17,23,27- heptaazotetracyclo [,12~]heptacose-1(25),2(27),3,5,8(26),9,11,21,23-nonane-16-one, or (ii) activin A and Wnt ligand; and (c) a factor selected from insulin and IGF-1.EFFECT: invention provides high efficiency of embryonic stem cells differentiation.20 cl, 9 dwg, 5 tbl, 5 ex

Bacterium strains lactobacillus rhamnosus, with a wide spectrum of antagonistic activity relating to pathogenic and conditionally pathogenic microorganisms // 2627166
FIELD: biotechnology.SUBSTANCE: strain of bacteria Lactobacillus rhamnoses 24 ds, which has antagonistic activity against pathogenic and opportunistic microorganisms, including against yeast fungi of the genus Candida, deposited in the All-Russian Collection of Industrial Microorganisms under the registration number VKPM V-12596 and can be used In the production of probiotic bacterial preparations, biologically active food additives, fermented and unfermented food products.EFFECT: improved strain properties.1 dwg, 7 tbl, 2 ex

Bacterium strains lactobacillus rhamnosus 7 ds, with a wide spectrum of antagonistic activity relating to pathogenic and conditionally pathogenic microorganisms // 2627165
FIELD: biotechnology.SUBSTANCE: strain of bacteria Lactobacillus rhamnosus 7ds, which has antagonistic activity against pathogenic and opportunistic microorganisms, including those with yeast fungi of the genus Candida, was deposited in the All-Russian Collection of Industrial Microorganisms under the registration number VKPM V-12598. The strain can be used in the production of probiotic bacterial preparations, dietary supplements, fermented and unfermented foods.EFFECT: improved strain properties.1 dwg, 7 tbl, 2 ex

Bacterium strains lactobacillus rhamnosus, with a wide spectrum of antagonistic activity relating to pathogenic and conditionally pathogenic microorganisms // 2627164
FIELD: biotechnology.SUBSTANCE: strain of bacteria Lactobacillus rhamnoses 1 ds, which has antagonistic activity against pathogenic and opportunistic microorganisms, including against yeast fungi of the genus Candida, deposited in the All-Russian Collection of Industrial Microorganisms under the registration number VKPM V-12597 and can be used in the production of probiotic bacterial preparations, biologically active food additives, fermented and unfermented food products.EFFECT: improved strain properties.1 dwg, 7 tbl, 2 ex

Anti-clusterin antibodies and antigen-binding fragments and their use for tumours volume reduction // 2627163
FIELD: biotechnology.SUBSTANCE: clusterin-binding antibody and an antigen-binding fragment thereof are proposed. Nucleic acids encoding variable regions of antibodies, an expression vector; a cell; a kit and a method for production of an antibody or an antigen-binding fragment thereof are described. Also a pharmaceutical composition for cancer cell growth reduction or tumour cells volume reduction; combination for cancer treatment, and a pharmaceutical composition comprising a chemotherapeutic agent; a method for cancer cell growth reduction or tumour cells volume reduction; a method for carcinomas treatment; kit for application in cancer treatment or detection; use of an antibody or an antigen-binding fragment thereof for tumour volume reduction. The invention may find further application for treatment of diseases associated with clusterin.EFFECT: antibody of the present invention is a humanized form of murine antibody 16B5 and has an approximately equal binding affinity for clusterin.28 cl, 16 dwg, 1 tbl, 6 ex

Insect-resistant and herbicide tolerant breeding stack of soybean event pdab9582.814.19.1 and pdab4468.04.16.1 // 2627161
FIELD: biotechnology.SUBSTANCE: invention relates to a method for controlling pests, such as Pseudoplusia includes (soybean looper), Anticarsia gemmatalis (velvet bean armyworm), Spodoptera frugiperda (antler moth). A transgenic soybean plant resistant to the specified pests, a transgenic soybean plant part, a soybean plant seed, soybean grain are also disclosed.EFFECT: invention allows to deal effectively with these pests.6 cl, 3 dwg, 12 tbl, 8 ex