Chemistry and metallurgy (C)

C            Chemistry; metallurgy(312744)
C01 - Inorganic chemistry(20496)
C06 - Explosives; matches(2052)
C07 - Organic chemistry(60703)
C13 - Sugar industry(2220)
C21 - etallurgy of iron(23017)
C30 - Crystal growth(2071)
Vulcanisable compositions based on nitrile rubbers containing epoxy groups // 2614384
FIELD: chemistry.SUBSTANCE: invention relates to chemical industry. Vulcanisable composition contains one epoxy group-containing hydrogenated nitrile rubber, which contains repeating units of one conjugated diene, one α,β-unsaturated nitrile and one or more additional copolymerisable of monomers; one Lewis base and/or Bronsted base as a cross-linking agent; one cross-linking accelerator. Lewis base and/or Bronsted base differ from compounds, which form a group of cross-linking accelerators.EFFECT: invention makes it possible to limit use of peroxide or sulphur-containing cross-linking agents in vulcanisable compositions, optimise residual compression deformation at high temperatures during prolonged stress.12 cl, 10 tbl

Lining method for cathode assembly of electrolyzer for primary aluminium production (versions) // 2614357
FIELD: metallurgy.SUBSTANCE: method includes filling the cathode assembly casing with heat insulation layer, building up a refractory layer, followed by packing layers, installing hearth and side blocks, followed by sealing the joints between them with cold ramming paste. In accordance with the first alternative of the presented method an elastic element made of compact fibreboard with the thickness of (2.5-4)⋅10-4 of the cathode width is installed between the heat insulation and refractory layers. According to the second alternative of the presented method flexible graphite foil is installed between the heat insulation and refractory layers and an elastic element of the abovementioned fibreboard is mounted beneath it.EFFECT: reduced power consumption during the electrolyzer operation by improving the stabilization of the thermal properties of the heat insulation in the basement, extended life of electrolyzers.6 cl, 5 dwg, 1 tbl
Titanium-based alloy and product made from it // 2614356
FIELD: metallurgy.SUBSTANCE: titanium-based alloy contains, wt %: aluminium 1.5-4.5;. vanadium 13.5-19.0; chrome 2.0-5.0; tin 2.0-4.0; molybdenum 0.5-2.5; zirconium 0.5-2.5; niobium 0.01-0.40; yttrium 0.005-0.150; titanium and impurities - the rest.EFFECT: alloy is characterized by high values of ductility, thermal stability, and creep strength in the thermally hardened condition, while keeping the fracture viscosity values.5 cl, 2 tbl, 4 ex
Titanium-based alloy and product made from it // 2614355
FIELD: metallurgy.SUBSTANCE: titanium-based alloy contains, weight %: aluminium 6.0-8.0, molybdenum - 0.4-1.3, tin - 1.5-3.5, zirconium 1.0-5.0 , iron - 0.05-0.4, niobium - 0.4-1.4, silicon - 0.1-0.4, tantalum - 0.2-1.0, tungsten - 0.3-1.3, beryllium - 0.01-0.15, titanium - the rest.EFFECT: alloy is characterized by high values of short-term strength.2 cl, 2 tbl, 4 ex
Gamma titanium aluminide-based alloy // 2614354
FIELD: metallurgy.SUBSTANCE: gamma titanium aluminide based alloy contains wt %: aluminium 29.0-33.0 , niobium 5.0-9.0, tungsten 1.0-2.0, molybdenum 2.0-4.0, vanadium 1.0-3.0, borium 0.003- 0.1, titanium - the rest. The molybdenum and tungsten weight ratio in the alloy is 2:1. The alloy may additionally contain the iron in the amount from 0.003 to 0.3 wt % and/ or oxygen in the amount from 0.003 to 0.15 wt %.EFFECT: increase of the absolute and specific values of ultimate strength and yield strength at temperatures of 20 and 750 degrees, the working temperature of the alloy increase up to 800 degrees by providing thermal stability of structure and high stress rupture strength.3 cl, 2 tbl, 7 ex
Ceramic mass // 2614341
FIELD: construction.SUBSTANCE: ceramic mass includes loam, expanded clay, an iron-containing additive and a burnable additive. As the iron-containing additive, a grounded ammonia production waste - a spent catalyst - is taken, and as a burnable additive - a liquid expanded polystyrene production waste (mother liquor), with the following ratio of the ceramic mass, wt %: loam 69.35-70.5 ; expanded clay 23-23.5; an iron-containing waste - ground spent ammonia production catalyst - to a specific surface area of 200 m2/kg 1.25-1.5; a liquid expanded polystyrene production waste with the density of 1.014 g/l 4.5-6.4.EFFECT: increasing the product strength after drying, burning, reducing heat and power costs due to reducing the burning temperature, preserving the architectural appearance of products.5 ex, 2 tbl
Complex organic-mineral additive for concrete // 2614340
FIELD: construction.SUBSTANCE: complex organic-mineral additive for concrete, including a superplasticizer of multifunctional action on the basis of sodium salts of polymethylenenaphthalenesulfonic acid "PFM-NLK", additionally comprises: soda-alcaline melting - a caprolactam production waste, fine brown coal - an associated waste generated on wagons bottom during transportation or in warehouses basements with the following ratio, wt %: superplasticizer - 20.15-20.7; soda-alcaline melting - 51.7-53.11; brown coal - 26.74-27.6.EFFECT: reduction of material and heat power costs, shortening the time of the concrete mix setting end without reducing the concrete workability and durability effect.4 tbl
Composition for manufacture of expanded aggregate // 2614339
FIELD: chemistry.SUBSTANCE: invention relates to production of construction materials, specifically to production of water glass-based expanded aggregates, intended for manufacture of lightweight concrete, and fill insulation materials. Composition for manufacture of the expanded aggregate comprises as follows, wt %: sodium water glass with a density of 1.41 g/cm3 50-75, sodium chloride, ground to a particle size below 0.3 mm 1-3, burnt rock, ground to a particle size below 0.14 mm 12-34, drill cuttings, ground to a particle size below 0.14 mm, and oxides, wt %: SiO2 - 26.2; Al2O3 - 4.5; Fe2O3 - 5.6; CaO - 28.3; MgO - 1.2; R2O - 0.8; ignition loss - 33.4, 10-15.EFFECT: composition for manufacture of expanded aggregates, with improved compression strength and softening temperature, industrial waste recycling.3 tbl
Thermally conductive polymer composite material // 2614334
FIELD: physics.SUBSTANCE: invention relates to thermally conductive dielectric materials and can be used for manufacture of heat-removing gaskets, tapes, sealants, filling compounds for computer memory chips, power electronic products, portable devices, power supplies and power converters, for which it is necessary to provide heat removal from heat-loaded elements and assemblies. A thermally conducting polymer composite material comprising a matrix based on silicone rubber or epoxy material with a filler in an amount of 0.1-80% by total weight of the thermally conductive composite material, wherein the filler is made in the form of aluminum oxide particles, the dimension of which is expressed in nanometers and micrometers, in combination with alumina nanofibers in an amount of 0.1-50% by total weight of the thermally conductive composite material and in the form of alumina nanofibers only in an amount up to 80% of the total weight of thermally conductive composite material, at that, the nanofibers are randomly arranged in the polymer matrix or oriented along the direction of heat flow from the heat-loaded surface.EFFECT: invention provides thermally conducting composite material with high thermal conductivity.3 ex

Coke cooling plant // 2614332
FIELD: oil and gas industry.SUBSTANCE: coke cooling plant contains a water bath, a rotating drum installed therein, the means for clinker loading and unloading. The rotary drum is designed as installed obliquely at the angle α relatively to the cylinder horizontal axis with end walls of the elliptical shape, at that the end wall at the loading device is flat and installed at the angle ψ to the drum rotation axis, and the end wall at the loading device is placed at the angle β to the drum rotation axis and made conical at that the angle β is not equal to the angle ψ. The cylinder spring of the waveform with round cross-sections of coils is mounted along the entire length of the drum, and the direction of the coils coincides with the direction of the drum rotation, which is equipped with the device for pitch changing by its stretching or compressing.EFFECT: increase of the cooling efficiency, the simplification of the structure assembly.5 dwg

ethod for producing thin nanodiamond film on glass substrate // 2614330
FIELD: chemistry.SUBSTANCE: thin nanodiamond film is obtained on a glass substrate by spraying the target material by a pulsed laser in a vacuum. The target is represented by detonation nanodiamond tablets, and the pulsed laser radiation source is represented by the laser with a wavelength of 1064 nm of a series of 13 to 20 pulses, with a pulse energy from 3.8 to 5.8 J and a duration from 1 to 1.5 ms. The process is accompanied by dispersing the detonation nanodiamond conglomerates up to individual nanocrystals and purification from impurities in the process of high-energy laser pulse impact. The resulting film is a two-dimensional polycrystalline nanodiamond aggregate.EFFECT: obtaining thin-film hardening coatings and creating nanostructured materials.9 dwg, 2 tbl, 5 ex
Composite antifriction material and its manufacturing method // 2614327
FIELD: metallurgy.SUBSTANCE: invention refers to antifriction materials. Composite antifriction material based on bronze-filled PTFE consists of tin and lead bronze, PTFE and ultradisperse diamonds at the following component ratio, wt %: PTFE 5-6; ultradisperse diamonds 0.065-0.187; the rest is tin and lead bronze. Method of material manufacturing involves preliminary mixing of PTFE and ultradisperse diamond powders. Then the mix is mixed with tin and lead bronze powder. Obtained three-component mix undergoes cold pressing at 8000-9000 kgf/cm2 followed by hot pressing at 355-400 °C and pressure of 4500-5500 kgf/cm2. Hot-pressed workpiece is cooled down to room temperature under the pressure of hot pressing.EFFECT: enhanced mechanical strength and tribo-technical parameters of composite antifriction material.2 cl, 2 tbl, 1 ex
ethod for liquid-phase synthesis of multi-component ceramic material in zro2-y2o3-gd2o3-mgo system for production of electrolyte for solid oxide fuel cell // 2614322
FIELD: chemistry.SUBSTANCE: invention may be used for production of electrolyte for solid oxide fuel cell. Liquid-phase synthesis of multi-component ceramic material in ZrO2-Y2O3-Gd2O3-MgO system is carried out by selecting salts ZrO(NO3)2⋅2H2O, Y(NO3)3⋅5H2O, Gd(NO3)3⋅6H2O and Mg(NO3)2⋅6H2O as initial reagents. Above salts are used to prepare diluted solutions. Reverse precipitation of hydroxides from respective salts is carried out using aqueous ammonia solution NH4OH till complete precipitation of all hydroxides and occurrence of the precipitate. Gel-like precipitate is filtered and frozen at -25°C for 24 hours. Synthesized powder of solid solution (ZrO2)0.92(Y2O3)0.03(Gd2O3)0.03(MgO)0.02 is subject to thermal treatment at 800°C. Then compacts are formed by the method of uniaxial pressing under pressure of 150 MPa. Above compacts are burned at a temperature of 1400°C in the tube heater with isothermal holding for 2 h and heating rate of 350-400°C/h.EFFECT: invention allows to lower precipitate agglomeration degree, increase its dispersivity and homogeneity and also hydroxide precipitation completeness.4 dwg, 2 tbl
Weldable alloy based on aluminium for meteoroid protection // 2614321
FIELD: metallurgy.SUBSTANCE: alloy contains wt %: zinc 2-8.5, magnesium 1.5-3.5, manganese 0.1-0.5, chromium 0.05-0.3, zirconium 0.05-0.3, hafnium 0.05-1.5, beryllium 0.0001-0.01, at least one element from the group: copper, titanium, nickel, cobalt up to 0.30 each, aluminium and inevitable impurities in an amount not exceeding 0.7 are the rest.EFFECT: due to uniform grain structure provides high resistance to impact with increase in strength, satisfactory ductility and weldability.3 cl, 5 ex, 2 tbl, 6 dwg
Heat resistant metal-ceramic coating and method of its application // 2614320
FIELD: machine building.SUBSTANCE: invention relates to machine building and can be used for production of parts with increased heat resistance. In heat-resistant metal-ceramic coating, consisting of metals high-melting oxides alternating layers separated with plastic metal compensation layers, high-melting oxides layers are additionally contain not more than 40 % of plastic metal, and compensating layers are additionally contain not more than 20 % of high-melting oxides. Each plastic metal compensating layer is made with thickness of not more than 100 nm and in 1.5–20 times less than ceramics layer thickness. As high-melting oxides layers hafnium oxide or zirconium oxide can be used, and compensating layers contain up to 50 % of rare-earth metals.EFFECT: alternating oxide and compensating layers are sprayed with smooth change of composition.4 cl

Clinker cooler // 2614295
FIELD: oil and gas industry.SUBSTANCE: clinker cooler comprises a water bath, a rotating drum installed therein, the means for clinker loading and unloading. The drum is designed as the obliquely installed respectively to its horizontal cylinder axis of rotation with flat end walls of elliptical shape, parallel to each other, perpendicularly or obliquely placed at the angle β to the horizontal drum axis of rotation, while maintaining parallel to each other, at that the major axes of the ellipses end walls are turned relative to each other at the angle ϕ and the spring of barrel shape with a circular coils section is fixed along the entire length of the drum, which equipped with device to change the turns pitch by its stretching or compressing.EFFECT: increase of the clinker cooling degree and expansion of the cooler process capabilities.4 dwg
ethod of blades forgings manufacturing from titanium alloys // 2614294
FIELD: metallurgy.SUBSTANCE: forgings manufacturing method from titanium alloys includes extrusion of billet under isothermal conditions at the same temperature of heating the billet and die and subsequent isothermal forging of the extruded billet. The extrusion and isothermal forging is performed at the heating temperature of the die and billet 800-830 °C±40 °C at average strain rate not more than 0.3 mm/s.EFFECT: reduce of alloy deformation resistance and increase of dimensional stability of dies, manufacturing of blade forgings with a fine-grained structure.1 dwg,1 ex

ethod of low-autogenous raw material processing in flash smelting furnaces // 2614293
FIELD: metallurgy.SUBSTANCE: according to method, low-autogenous raw material processing in flash smelting furnace comprises supplying a metal-containing charge and flux as charge-gas flame to reaction zone of the said furnace by stream of oxygen or air enriched with oxygen, charge melting occurs to form a molten matte and slag, their separation by settling, separate liquid melting products and gases outputing. Charge composition comprises carbon containing reducer with fineness of 3-15 mm in an amount of 0.4 to 5 tons per hour in at volume ratio of 1:30 to 1:2 relative to flux.EFFECT: increased productivity of flash smelting process, reduced probability of scull formation in uptake and slag bath of furnace, and also reduced losses of non-ferrous metals with waste slag.2 cl, 3 tbl
ethod of cyclic gas nitration of structural alloy steel parts // 2614292
FIELD: metallurgy.SUBSTANCE: method of cyclic gas nitration of structural alloy steel parts includes part heating in the furnace up to the temperature of 540-650 °C, isothermal holding at the heating temperature, during which the saturating atmosphere replacement is performed cyclically in two stages in each cycle, and subsequent cooling together with the furnace in the ammonia-base atmosphere. The nanoscale copper film is prelimenary applied to the part surface, and the mentioned heating is conducted in the ammonia and carbon dioxide atmosphere at a volume ratio of these components 1:1. The first cycle stage of isothermal holding is carried out in saturating ammonia atmosphere in a mixture with water vapour at a ratio of volumes of mentioned components 1:1 and obtaining the copper oxide film on the part surface. The second step of the mentioned cycle is carried out in saturating ammonia atmosphere in the mixture with carbon dioxide at the ratio of mentioned gas volumes 1:1, and with the copper oxide recovery up to forming the pure copper on the part surface. In the invention particular case, the thickness of the applied nanoscale copper film is within the range of 150-200 nm.EFFECT: increase of metal ceramics solid area thickness up to a specified value in the diffusion nitrided layer, obtained at the parts surface from structural alloyed steel without the nitriding process duration increase and without reducing its hardness.2 cl, 1 tbl, 5 ex
ethod for producing prussian blue/polypyrrole composite coatings with stable electro chromic transition // 2614290
FIELD: chemistry.SUBSTANCE: invention relates to methods for preparation of stable electrochromic coatings based on Prussian blue and a conductive polymer component. It can be used for production of electrochromic coatings on optically-transparent electrodes for architectural, building and automotive applications. Disclosed a method for production of Prussian blue- polypyrrole nanostructured composite coating with stable redox- and electrochromic transition on the surfaces of optically-transparent electrodes by a single-stage sedimentation in a mixed solution of ferrous nitrate (III) and hexacyanoferrate (III) as oxidising agents, and pyrrole monomer with a nitrate supporting electrolyte as a reducing agent with pH 1.0±0.2, within 48 hours.EFFECT: invention provides a simple single-stage method for production of Prussian blue- polypyrrole electrochromic nanostructured composite coatings on optically-transparent electrodes.2 cl, 4 ex
ethod for producing graphene film on substrate // 2614289
FIELD: chemistry.SUBSTANCE: substrate - a X-cut-off of piezoelectric crystal, e.g., La3Ga5.5Ta0.5O14, with planes (110) parallel to the crystal surface is placed into a quartz reactor. The reactor is pumped out to 10-3-10-8 Torr and heated up to 900-1450°C. The reactor then filled with carbon gases, e.g. acetylene, methane or ethylene, to achieve pressure of 10-10-1 Torr. After 15-100 min. the reactor is pumped out again to achieve pressure of 3·10-6 Torr while cooling it down to the room temperature.EFFECT: process simplification, temperature reduction, production of uniform high-quality graphene films.5 cl, 2 dwg, 3 ex

ethod of integrated municipal sewage water treatment and sediment reworking in villages withunsewered areas // 2614288
FIELD: chemistry.SUBSTANCE: method of integrated municipal sewage water treatment comprises equalization of consumption to hourly average consumption, mechanical sewage water treatment in sediment tanks, biological treatment in multistage bioreactors using biocenoses - microorganisms attached to the fiber brush nozzle, advanced sewage water treatment in the aerobic bioreactors and processing of precipitations excreted during sewage water treatment. Sedimentation is performed in the clarifier with flocculation chamber with natural aeration, precipitations of sewage water are fed from the clarifiers into anaerobic digester with a temperature of 15°C, while biological treatment is performed in nitri-denitrification bioreactors with biocenoses attached to the brush nozzle of polyamide super slim fibers with recirculation of treated water from the second nitrification stage to the denitrification stage, reclaimed water from the nitri-denitrification and advanced treatment bioreactors is fed into the consumptrion equalizing tank with supernatant water of digesters.EFFECT: quaility improvement of the water treatment, process simplification.3 dwg, 1 ex

Fluid cleaning system // 2614287
FIELD: technological processes.SUBSTANCE: invention relates to the cleaning systems and/or desalting the liquid, preferably water. Fluid purification system contains a supply line of the original fluid with mounted supply valve source fluid, connected to the filtration unit, which comprises liquid cleaning means with an inlet for source liquid and outputs for the purified and drain liquid; the fluid mixing device, pressure means support unit, mixing line for the source liquid concentrate, that is formed during the fluid treatment, in a liquid cleaning means, recycling line, the purified fluid line, the fluid drain line and the control unit associated with means of pressure support, pressure change control means, and source liquid supply valve . The filtration unit is adapted to stabilize the pressure in the fluid purification means at periodic fluid flow drainage to drain the fluid velocity, exceeding the performance of pressure support means, disposed on the source liquid line upstream of the device mixing fluid, made in the form of a pressure vessel, which is connected with a line mixing original liquid and concentrate, formed during the filtering process, and simultaneously adjusting of the concentrated fluid volume in the mixing apparatus due to the relationship of the control unit to support the pressure means in the fluid mixing apparatus, performed in a pressure vessel and with means of maintaining the circulation of fluid flow located in the line feeding the starting fluid mixture and concentrate The recirculation line through the connection node is connected to the source liquid supply line, after pressure suuport means and the mixing line of the source liquid and the concentrate, which is connected to the mixing device of the source liquid and the concentrate.EFFECT: invention increases the life of the fluid treatment system, increases efficiency, and reduces the initial fluid energy costs.7 cl, 1 dwg
Adhesive composition // 2614285
FIELD: chemistry.SUBSTANCE: adhesive composition contains shellac, ethyl alcohol, dibutyl phthalate at the following ratio, wt %: shellac 45, ethyl alcohol 45, dibutyl phthalate 10.EFFECT: adhesive composition is suitable for the temporary fixation of optical components groups together with the possibility of their multiple fastening for further processing.3 tbl

Fine water filter // 2614284
FIELD: chemistry.SUBSTANCE: fine water filter comprises the housing with a cover and a bottom, the central vertical tube with the upper drainage and distribution system, the two-layered granular loading, the middle drainage and distribution system, the reservoir for supplying compressed air, the fitting for hydraulic discharge, the technological hatches, the plunger, and the pipe for removing filtered water. Wherein the filter is equipped with the additional drainage and distribution system, which is located under the middle drainage and distribution system and on which the single-layered granular loading is placed, the pipe for supplying water for flushing and the pipe for removing water after flushing the single-layered granular loading, wherein the pipe for supplying the water for flushing the two-layered granular loading is arranged lower than the middle drainage and distribution system.EFFECT: increasing the water purification efficiency.2 cl, 1 dwg
Process for continuous production of halogen-free thermoplastic elastomer compositions // 2614277
FIELD: chemistry.SUBSTANCE: invention relates to thermoplastic elastomer compositions and methods for continuous production of said compositions. continuous method for production of dynamically cured thermoplastic elastomer comprises mixing at first stage of a mixing device of continuous action a thermoplastic resin, a halogen-free elastomer, with polyolefin content of more than 3.5 mol%, which is modified with unsaturated carboxyl compound, and a curing agent. At second stage a curing system is added, which is suitable for use with halogen-free elastomer for dynamic vulcanisation.EFFECT: invention enables to obtain materials with improved relative elongation at break and ultimate tensile strength.15 cl, 2 tbl
ethod for preparation of collagen product // 2614273
FIELD: pharmacy.SUBSTANCE: invention refers to processing and cosmetics industris, namely to a method for production of a collagen product with the following characteristics: reception of skins of freshwater fish such as silver carp, carp, grass carp, mirror carp, manual or machine cleaning from scale, muscle tissue, fins and mechanical impurities, washing for 30 minutes in drum type machines with a solution of soap and then in water - to remove residual soap, the rinsed skins are subjected to peroxide and alkaline treatment, for which they are poured with a mixture of hydrogen peroxide with mass concentration of 3% and sodium hydroxide with mass concentration of 3 % and kept at 20-25°C for 1.5-2 hours and liquor ratio of 1:4, the treated skins are washed with flowing water at 8-12°C for 10-15 min, then the skins are poured with citric acid solution with mass concentration of 6% and kept for 6 days, the resulting mass is homogenized, packaged in polyethylene and stored at 0…+4°C for° max. 4 months.EFFECT: increase of collagen product quality indicators: increase in collagen output, improved organoleptic properties, reduced complexity and cost by reducing the number of process steps and reagents, intensification of the pre-treatment process and raw materials degreasing.1 tbl, 2 ex
ethod for dielectric microcontainer administration into a mammal cell using femto-picosecond laser pulses // 2614269
FIELD: biotechnology.SUBSTANCE: optical trap for microcontainer is created using a focused laser beam with a wavelength of 830 nm. The microcontainer is brought into contact with the cell membrane by exposing it to laser pulses trains with a wavelength of 780 nm, with subsequent cutting of cell membrane by focused laser pulses and controlled introduction of the microcontainer into the cell using an optical trap.EFFECT: invention allows to deliver the microcontainer with genetic material to the given mammal cell coordinate with high precision.4 cl
ethod for determining toxicity of chemicals generating active oxygen forms // 2614267
FIELD: chemistry.SUBSTANCE: method provides adding riboflavin to the final concentration of 1⋅10-5 mg/ml - 1⋅10-3 mg/ml into the Escherichia coli K12 MG1655 culture with the pColD-lux plasmid, in which the bioluminescence lux operon of marine photobacteria Photobacterium leiognathi, Vibrio fischeri or Photorabdus luminescens is placed under the control of the pColD promoter. Adding the test substance and determining the luminescence intensity of the resulting suspension and the control are carried out. The damaging effect of the test substance is estimated by the suspension luminescence intensity deviation from the control.EFFECT: increasing the induction factor.4 cl, 1 dwg, 2 ex
ethod for preparing dosage for production of sparkling wines // 2614265
FIELD: food industry.SUBSTANCE: in bottling-stable white or red table wine stocks or in champagnised wine sugar at the rate of 700-800 g/dm3 and organic acids: citric, lactic and malic acid in a ratio of 1:1:0.5 are added n an amount providing the achievement of the mass titratable acids concentration in the dosage of 6.0-8.0 g/dm3. The mixture is separated into two parts, one of which is heated to 40°C, and into the other part the sedimentary yeast settling in an acratophore during champagnisation is introduced in an amount of 5-10% by volume of the dosage, and heated to 50°C. Both parts of the dosage are mixed at the ratio of 3:1, maintained for 30 days and filtered.EFFECT: invention allows to reduce the time for the dosage preparation and maintaining process up to 30 days instead of 100 days and to improve its organoleptic and physico-chemical parameters.3 ex

Optimized recombinant protein encoding gene - human interferon beta analog // 2614264
FIELD: biotechnology.SUBSTANCE: nucleotide sequence encoding a recombinant protein - interferon beta analog, optimized for expression in E. coli cells. E. coli BL-30-B cell, intended for interferon beta recombinant protein production is obtained by transformation of E. coli BL (DE3) cells by rET30a plasmid containing the said nucleic acid. E. coli BL-32-B cell, intended for interferon beta recombinant protein production is obtained by transformation of E. coli BL (DE3) cells by rET32a plasmid containing the said nucleic acid.EFFECT: invention provides a recombinant protein.3 cl, 5 dwg, 9 tbl, 9 ex
Trametes hirsuta strain - producer of ethanol // 2614263
FIELD: biotechnology.SUBSTANCE: Trametes hirsuta MT-24.24 basidiomycete strain has the ability to produce ethanol. Trametes hirsuta basidiomycete strain is deposited in the Russian National Collection of Industrial Microorganisms under accession number VKPM F-1288 and can be used in medicine and food industry.EFFECT: invention allows to obtain high quality alcohol containing no impurities such as acetone and isopropanol.1 tbl, 5 ex
Strain of bacteria micrococcus luteus 805 - producer of site-specific methyl-dependedn endonuclease mluvi // 2614262
FIELD: biotechnology.SUBSTANCE: strain Micrococcus luteus, deposited under registration number B-12410 in The All-Russia Collection of Industrial Microorganisms of GosNIIGenetika FSUE is the producer of the site-specific methyl-dependent endonuclease MluVI. The invention provides a new MD-endonuclease enzyme which recognizes and cleaves both strands of the DNA sequence 5'-GCGC^NGCGC-3'/3'-CGCGN^CGCG-5' before the central nucleotide (N) with formation of single-nucleotide 5'-protruding ends in the presence in a given sequence of at least six C5-methylized cytosines and can be used for large-site-specific hydrolysis of DNA containing C5-methyl-cytosine bases.EFFECT: increase the efficiency of strain application.2 dwg, 1 tbl, 3 ex
Two-layer, flat, transparent hydrogel substrate for long-term cell cultivation // 2614260
FIELD: biotechnology.SUBSTANCE: two-layer, flat, transparent hydrogel substrate is proposed for long term cell cultivation and the method to get it. The substrate in the hydrated state has a thickness in the range of 25-500 microns, and the radius of menisci curvature of 10-150 microns. The hydrogel substrate includes a structure forming layer consisting of a three-dimensional collagen hydrogel and an upper protein layer. The three-dimensional hydrogel comprises collagen of type I, collagen of type III and proteins that promote cell adhesion and migration. The upper protein layer contains collagen of type IV, laminin and fibronectin. The method of substrate preparing comprises mixing of solutions of collagen of type I, collagen of type III and proteins that promote cell adhesion and migration and further gelation and vitrification, followed by proteins immobilization on the surface.EFFECT: preservation of phenotype by endothelial cells and epithelial cells in their long-term cultivation.13 cl, 1 dwg, 3 tbl, 8 ex

ethod of producing l-amino acids // 2614258
FIELD: biotechnology.SUBSTANCE: invention relates to biotechnology. Method of producing L-lysine using recombinant coryneform microorganism able to produce L-lysine is disclosed, where recombinant coryneform microorganism is transformed by means of insertion of acetate-inducible promoter below stop codon of target gene on chromosome for attenuation of target gene expression and enhancing of ability of recombinant coryneform microorganism to produce L-lysine, where target gene is gene located in nodal point of L-lysine biosynthesis path.EFFECT: invention increases production of L-lysine in comparison with parent strain of microorganism.4 cl, 2 dwg, 12 tbl, 13 ex
ethod of detecting of cell culture contamination with viruses by immunoperoxidase method // 2614256
FIELD: biochemistry.SUBSTANCE: invention relates to biochemistry. Method of detecting contamination with viruses of cell culture by immunoperoxidase method is described, involving reaction of antigen with labelled antibodies in clean cell culture and accounting of reaction results by color density of formed complex under microscope. Wherein reaction uses tablets with analyzed cell culture, contaminated with diarrhoea virus, and after adding into tablet wells of 0.10–0.12 ml of specific homologous serum diluted by 1:64 – 1:128 it is incubated, washed, then 0.10–0.12 ml of anti-specific immunoenzymometric conjugate is introduced, consisting of peroxidase marked antibodies against globulins of cattle blood serum, it is incubated, washed, substrate mixture is introduced consisting of 5-aminosalicylic acid and hydrogen peroxide, and 30–40 minutes later results are accounted under light microscope by formation of brown color in virus-containing cells. Invention improves control system of cell lines latent contamination with viruses used in laboratory practice and biological industry.EFFECT: invention allows to prevent biological contamination and is extremely important stage in production of vaccines and diagnostic preparations; this method can be used for certification at absence of viruses in again coming cell cultures; invention is practically feasible, cheap method of monitoring of cell cultures for content of virus and may be recommended for extensive use both in scientific research and practical virological laboratories, and in production in making diagnostic preparations and vaccines.1 cl, 2 ex

Automated extraction of nucleic acids by size // 2614255
FIELD: biochemistry.SUBSTANCE: invention relates to biochemistry. Invention is described, which includes method for selection of nucleic acids by size. Method involves stages of movement of nucleic acids from sample via channel by means of electrophoresis, automatic tracking of movement of reference fraction of nucleic acids via channel, assessment of estimated time of arrival of target fraction of nucleic acids in hole for extraction in channel, extraction of fluid medium containing target fraction from hole to extract at estimated time of arrival.EFFECT: invention extends range of products for selection of nucleic molecules by size.44 cl, 15 dwg

Diagnostic markers // 2614254
FIELD: biochemistry.SUBSTANCE: invention relates to biochemistry. Method of determining sensitivity of tumor cell growth to inhibition by EGFR kinase inhibitor is disclosed, which involves determining ERBB2 gene methylation status in tumor cell sample, reduced level of methylation ERBB2 gene means, that less than 50 % of possible methylation sites in part of ERBB2 gene are methylated, and indicates that growth of tumor cells is sensitive to inhibition by inhibitor EGFR. Method of detecting patient with malignant tumor is also described, favorable effect on which can have treatment with help of EGFR inhibitor. Method of selecting therapy for patient with malignant tumor is described. Invention extends range of methods for prediction of response to anticancer therapy.EFFECT: method of determining ERBB2 gene overexpression in cell is disclosed.31 cl, 8 dwg, 6 ex

icroorganisms producing o-acetyl homoserine and method for o-acetyl homoserine production using this microorganism // 2614253
FIELD: biotechnology.SUBSTANCE: this microorganism is characterized by weakened or inactivated endogenous citrate synthase activity. As a result of this modification, the is microorganism capable of producing O-acetyl homoserine with high yield. The invention also relates to a method for O-acetyl homoserine production. This method comprises culturing of the said microorganism and separation of O-acetyl homoserine produced by culturing of this microorganism.EFFECT: invention allows to obtain O-acetyl homoserine with high yield.7 cl, 2 dwg, 10 tbl, 4 ex

Alpha-v beta-8-binding antibodies // 2614252
FIELD: pharmacy.SUBSTANCE: antibodies with high affinity for β8-subunit αvβ8 proposed. Pharmaceutical composition comprising such antibodies, and methods of their application are also described. The proposed group of inventions can be used in medicine.EFFECT: improved antibody properties.23 cl, 14 dwg, 19 ex
ethod for substituted quinolines production from aniline, 1,2-diols and ccl4 under iron catalysts // 2614251
FIELD: chemistry.SUBSTANCE: invention relates to a method for preparation of substituted quinolines of the formula shown below , where R1=CH3, R2 and R3=H; R1=CH2CH3, R2=CH3 and R3=H; R1=CH2CH3, R2=H and R3=CH3; R1=CH2CH2CH3, R2=CH2CH3 and R3=H; X=H, o-CH3, "м"-CH3, "п"-CH3, o-C2H5, o-Cl, "м"-Cl, "п"-Cl, "п"-OMe, o-OH, from aniline, wherein the substituted anilines of formula XC6H4NH2 where X is as defined above, is reacted with 1,2-diols (1,2-ethylene glycol, 1,2-propanediol or 1,2-butanediol) and CCl4 in the presence of a catalyst selected from FeCl3⋅6H2O, FeCl3, FeCl2⋅4H2O, Fe(C5H5)2, Fe(acac)3, Fe(OAc)2 and Fe2(CO)9, at a molar ratio [catalyst]:[aniline]:[CCl4]:[1,2-diol] = 1:100: 200:400, at 150 °C for 8 hours.EFFECT: process for preparation of quinoline derivatives that are raw materials for corrosion inhibitors, dyes and medicines production from available initial reactants.1 tbl, 35 ex
ethod for methyl n-[3,4-dialkyl-5-(n-methoxy-n-oxoalkyl)-2-furyl] alkanoates production // 2614250
FIELD: chemistry.SUBSTANCE: invention relates to a method for methyl n-[3,4-dialkyl-5-(n-methoxy-n-oxoalkyl)-2-furyl]alkanoates production by the general formula where R = C2H5, C3H7, C4H9; R'=(CH2)nCOOCH3, n=5, 6, by reaction of dialkyl acetylenes of the general formula R-C≡C-R, where R=C2H5, C3H7, C4H9, with carboxylic acid esters in the presence of magnesium (Mg, powder) and Cp2TiCl2 catalyst with methyl esters α,ω-dicarboxylic acids CH3CO2(CH2)nCO2CH3, where n=5, 6, at the molar ratio of RC≡CR : CH3CO2(CH2)nCO2CH3:EtAlCl2 : Mg:Cp2TiCl2 = 10:20(50-70):40:(1.8-2.2) in tetrahydrofuran under argon atmosphere at 60°C and atmospheric pressure during 4-8. Methyl n-[3,4-dialkyl-5-(n-methoxy-n-oxoalkyl)-2-furyl]alkanoates (1) yield is 54-76%.EFFECT: compound are important as initial synthons for biologically active compounds creation for medical and agricultural purposes, exhibiting antibacterial, antiviral, anti-inflammatory, antifungal, antitumor, analgesic, anticonvulsant properties.1 tbl, 1 ex

Composition for producing hydrogel based on polyvinyl alcohol for immobilization of microorganisms // 2614249
FIELD: chemistry.SUBSTANCE: composition for producing hydrogel based on polyvinyl alcohol for the immobilization of microorganisms is described, consisting of a polyvinyl alcohol and a catalyst of cerium-ammonium nitrate crosslinking, characterized in that the initial components are taken in the molar ratio of EVOH: (NH4)2Ce(NO3)6 (160-110):(1-1.3).EFFECT: ensuring formation of water-insoluble hydrogel for immobilization of microorganisms, improved accuracy and sensitivity of the assay using the biosensor.18 cl, 1 dwg,1 tbl, 1 ex

ethod for trimethyl substituted furodihydroquinolines synthesis // 2614248
FIELD: chemistry.SUBSTANCE: invention relates to a method for 7,7,9-trimethyl-6,7-dihydro-furo[3,2-ƒ]quinoline synthesis, comprising the 5-aminobenzofuran condensation with acetone in the presence of n-toluenesulphonic acid and magnesium perchlorate at a temperature of 70-125°C in an autoclave. At that, 5-aminobenzofuran is obtained by a sequence of steps consisting of: a - n-anisidine acylation with acetic anhydride to give N-(4-methoxyphenyl)acetamide; b - N-(4-methoxyphenyl)acetamide obtained in step interaction with chloroacetyl chloride to give N-[3-(chloroacetyl)-4-hydroxyphenyl]acetamide; c - dihydrofurane cycle circuit closure by treating the product obtained in step b by sodium acetate and subsequent intermediate reduction without isolation from the reaction mixture by sodium borohydride to yield N-(3-hydroxy-2,3-dihydro-1-benzofuran-5-yl)acetamide; d - dehydration of the product obtained in step c by treatment with n-toluenesulphonic acid and magnesium perchlorate to yield N-1-benzofuran-5-yl acetamide; e - acidic hydrolysis of the product obtained in step d to yield 5-aminobenzofurane. The method may be used as the basis for synthesis of various derivatives of furodihydroquinolines (FDHQ) trimethyl substituted by quinoline ring, that are of practical interest as potential sensitizers for application in PUVA-treatment of skin diseases, and they may be used in various fields of technology as antioxidants and antiozonants .EFFECT: simplification and cheapening of the desired product obtaining process and increase of its total output compared with the prototype.2 dwg, 2 ex

ethod of producing enantiomerically pure (s)-amino acids on basis of [(s)-bpb-gly]ni(ii) complex, directly bonded with fullerene core through α-carbon atom in form of chiral (f,ta) and (f,tc) 1,4-adducts of [60]fullerene // 2614247
FIELD: chemistry.SUBSTANCE: invention relates to a method for stereoselective synthesis of (S)-α-fullerenylglycine, wherein α-carbon atom of an acid is directly bonded with a fullerene core, in form of complex of Ni(II) of type of Schiff base with an auxiliary [(S)-BPB] ligand in form of chiral (f,tA) and (f,tC) 1,4-adducts of formula (I). Method includes steps A–E. Step A comprises modifying an initial amino acid by its incorporation in form of a Schiff base into coordination sphere of nickel complex with an auxiliary chiral ligand ((S)-2N-(N'-benzylprolyl)aminobenzophenone) [(S)-BPB-Gly]Ni(II). Step B comprises deprotonation of amino acid fragment of obtained complex. Step C comprises stereoselective reaction of anionic (deprotonated) complex with fullerene to form fullerene carbanion. Step D comprises reaction of fullerene carbanion with an electrophile, such as allyl- or benzyl halide, with formation of diastereomeric mixture of target fullerene (f,tA) and (f,tC) 1,4-adducts, containing (S)-α-amino acid fragment, directly bonded with fullerene core through α-carbon atom, in form of Schiff base in coordination sphere of Ni(II). Step E comprises separating and cleaning fullerene-containing adducts by column chromatography on silica gel to obtain a mixture of target fullerene (f,tA) and (f,tC) 1,4-adducts, containing (S)-α-amino acid fragment in coordination sphere of Ni(II). Invention also relates to a mixture of diastereomers, consisting of (f,tA) and (f,tC) 1,4-adducts, and optically pure (f,tA) and (f,tC) adducts.EFFECT: method enables stereoselective synthesis fullerenylglycine derivative having (S)-configuration of α-amino acid stereocentre.8 cl, 1 dwg, 6 ex (I)
Epoxyurethane binder with increased heat and temperature resistance // 2614246
FIELD: chemistry.SUBSTANCE: binder comprises an epoxy component and an aromatic polyisocyanate. The epoxy component is a mixture of the polyfunctional epoxy resin and the phenyl glycidyl ether, which reacts to the aromatic polyisocyanate in the presence of a amine hardener. Said amine hardener is triethanolamine.EFFECT: using said epoxypolyurethane bindings allows to produce organic, basalt, carbon and fiberglass plastics with high thermal and heat resistance, chemical resistance in corrosive media, as well as the low porosity; the invention also allows to improve the sanitary work conditions and the environmental situation at production due to the technology of manufacturing filler-reinforced plastics without the use of solvents.4 tbl, 2 ex
ethod for hybrid plasmon-luminescent markers production // 2614245
FIELD: chemistry.SUBSTANCE: method consists in formation of metal plasmon nanoparticles on the inorganic luminescent nanoparticles surface, where nanoparticles are previously doped by rare earth metal ions. Plasmon nanoparticles are prepared by reduction of liquid solutions. The colloidal solution of inorganic luminescent nanoparticles in 1.2-dichloroethane is mixed with a supramolecular complex solution [{Au10Ag12(C2Ph)20}Au3PPh2(C6H4)3PPh2)3][PF6]5 in 1,2-dichloroethane to obtain a liquid solution, the liquid solution is recovered by laser radiation with a wavelength corresponding to the absorption band of the supramolecular complex, a power density of from 0.1 to 1 mW/cm2at laser exposure time of 10-60 minutes.EFFECT: invention enables high chemical purity of markers and small number of processing steps.5 dwg, 3 ex
ethod for waste mineral motor oils purification // 2614244
FIELD: chemistry.SUBSTANCE: purification of waste mineral motor oil using monoethanolamine, orthophosphoric acid as coagulants and isopropyl alcohol, methyl ethyl ketone as solvents, purification is carried out in two stages, the first stage includes introduction of 1 vol. % of monoethanolamine and 1 vol. % of isopropyl alcohol into the waste oil, followed by heating to 110°C and centrifugation, and the second stage includes introduction of 1 vol. % of monoethanolamine and 1 vol. % of a reagent, consisting of 94% isopropyl alcohol, 3% phosphoric acid and 3% methyl ethyl ketone, into the purified oil, and the mixture is heated to 120°C and centrifuged.EFFECT: application of the proposed method for waste mineral motor oil purification allows to completely remove insoluble impurities, oxidation products, resins due to two-stage purification, the refined oil has a high degree of clarification.1 tbl
Yeast transformant schizosaccharomyces pombe, producing lactic acid (versions) method for production thereof (versions), method for lactic acid microbiological synthesis using this transformant // 2614233
FIELD: biotechnology.SUBSTANCE: transformant with gene ldh, encoding lactate dehydrogenase from Lactobacillus acidophilus or an enzyme with amino acid sequence homologous to it by at least 93% are proposed. Also a transformant with gene ldh, encoding lactate dehydrogenase from Lactobacillus acidophilus or an enzyme with amino acid sequence homologous to it by at least 93% are proposed, wherein one or several genes encoding enzymes involved in the ethanol biosynthesis pathway are deleted or inactivated. A transformant with gene ldh, encoding lactate dehydrogenase from Lactobacillus acidophilus or an enzyme with amino acid sequence homologous to it by at least 93% are proposed together with gene ldh encoding lactate dehydrogenase from Lactobacillus plantarum or Lactobacillus pentosus. A transformant with gene ldh, encoding lactate dehydrogenase from Lactobacillus acidophilus or an enzyme with amino acid sequence homologous to it by at least 93% are proposed together with gene ldh encoding lactate dehydrogenase from Lactobacillus plantarum or Lactobacillus pentosus,wherein one or several genes encoding enzymes involved in the ethanol biosynthesis pathway are deleted or inactivated. Also, versions of the preparation method for these transformants and the method for microbial synthesis of lactic acid are proposed.EFFECT: efficient production of lactic acid at low concentrations of by-product.13 cl, 10 dwg, 8 ex
Niobium-base alloy for forming 3d-products of complex shape and coatings // 2614230
FIELD: metallurgy.SUBSTANCE: composite niobium-based alloy, using for forming of 3d-products of complex shapes and the thermal barrier coatings contains wt %: zirconium: 1.36-2.04, vanadium: 2.64-3.96, indium: 1.0-1.6, cerium: 0.3-0.6, yttrium: 0.3-0.6, lanthanum: 0.3-0.6, tungsten carbide: 3.0-5.0, niobium - others. The alloy is obtained by introducing vanadium and zirconium in the form of intermetallic V2Zr in the amount of 4-6 wt %: indium - in the form of intermetallide InNb3 in the amount of 5.0-8.0 wt %, and tungsten carbide has fraction of 30-100 nm.EFFECT: the alloy is characterized by high microhardness, which provides high durability in a wide temperature range, in particular from positive to negative temperatures.2 cl, 1 tbl, 2 ex
 
2551085.
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