Plasma and serum (A61K35/16)

Combination of growth factors, cytokines, antibacterial/antiviral factors, stem cells factors, c3a/s4a complement proteins, immunoglobulins and chemotactic factors // 2642650
FIELD: pharmacology.SUBSTANCE: combination of cytokines, growth factors, chemotactic factors, stem cell factors, protein complement, immunoglobulins and antibacterial/antiviral factors, taken in a specific ratio and with a specific components composition for treatment of diseases, requiring reconstruction and regeneration of tissues. A pharmaceutical composition for treatment of diseases requiring restoration and regeneration of tissues.EFFECT: combination described above and the pharmaceutical composition can effectively regenerate and restore tissues.9 cl, 2 dwg, 11 tbl, 9 ex
ethod for treatment of heavy liver lesion with posttraumatic coagulopathy // 2639422
FIELD: medicine.SUBSTANCE: right gastro-omental vein is excised and catheterized, through which fresh frozen plasma and platelet concentrate in a ratio of 1:1 or fresh platelet-rich plasma is transfused into the portal vein system with additional administration of 1 g tranexamic acid for 10 minutes and further administration every 8 hours for 1 g till hyperfibrinolysis reduction. Treatment is carried out under the control of thromboelastography, aggregation, APTT, PT, MHO and fibrinogen level.EFFECT: method allows to achieve maximum hemostatic effect in the wound, to correct the key pathogenetic links of post-traumatic coagulopathy and to reduce the risk of early and distant complications.1 cl
ethod for local treatment of trophic ulcers of venous etiology // 2639123
FIELD: medicine.SUBSTANCE: method includes administration of platelet-enriched auto plasm and regional administration of lymphostimulating drugs at optimal doses with an effective course of treatment, with the autoplasm being administered sub bulcerously.EFFECT: application of the invention allows to achieve the restoration of microcirculation in the lower limbs area, more rapid disappearance of perifocal swelling, faster onset of necrolysis, emergence of granulation tissue in ulcers, accelerated reparation and epithelialization of a trophic ulcer.6 cl, 1 dwg

ethod for obtaining of two-component preparation for treatment of joints damage by low-invasive introduction into joint bag and preparation obtained by this method // 2638796
FIELD: medicine.SUBSTANCE: method for obtaining of a two-component preparation for treatment of joint damage by low-invasive insertion of the said preparation into the joint bag, in the form of two components, the main and initiating, consisting in the fact that, in order to obtain the main component of the preparation, the blood plasma is transferred with a syringe and a transient filter with a pore size sufficient to ensure sterility, to one of the departments of the cryopack and kept until complete freezing, then the frozen blood plasma is partly defrosted, the first thawed plasma fraction is transferred to the empty compartment of the cryopack and removed from the cryopack by a syringe connected to the pack port, and the cryoprecipitate of the blood plasma remaining in the cryopack is thawed, then the cells which are chondrocyte precursors, are resuspended in the cryoprecipitate of the blood plasma in an amount sufficient to provide a therapeutic effect, and to make the initiating component of the preparation, solution of calcium chloride and thrombin is mixed in a balanced saline solution with addition of aminocaproic acid in an amount not exceeding the maximum recommended dose for a single administration in the joint bag, under certain conditions. A two-component preparation for treatment of joint damage.EFFECT: method allows for cells localization at the site of administration, allowing the cells to migrate from the glue to the damage site and maintain functional activity.8 cl, 5 dwg
ethod for treatment of oral mucosa chronic inflammation with hyperkeratosis elements in young persons // 2637413
FIELD: medicine.SUBSTANCE: invention is intended for treatment of oral mucosa chronic inflammation with hyperkeratosis elements (lichen acuminatus of erosive-ulcerous form, flat form of leukokeratosis). Injection of platelet-enriched plasma 0.1-0.5 ml per mm2 is performed once a week, as a course of 3 procedures, and of placental drug "Laennek" 0.1-0.2 ml per mm2, 1 injection with an interval of 2 days, as a course of 5-10 injections, the injections are carried out under the morphological elements located on the oral mucosa and "Laennec" is additionally injected intravenously, dropwise, in a dose of 4 ml dissolved in 250-500 ml of physiological solution, for the course of 10 procedures with an interval of 2 days.EFFECT: application of the method provides long-term remission due to a complex effect on the main links of the disease pathogenesis, which is manifested in the reduction of inflammatory response, normalization of local immunity, microbial contamination of the oral fluid and stabilization of free-radical oxidation of the oral fluid.2 ex
ethod for complex treatment of endogenous uveitis followed by macular edema // 2635083
FIELD: medicine.SUBSTANCE: alternately, on the side of the affected eye, pterygopalatine and BTE drug injections are performed and ultraviolet irradiation of blood is performed. Pterygopalatine injections are performed by introducing platelet-enriched autoplasma at a dose of 3-5 ml by a course of 3-4 injections with an interval of 72-96 hours. BTE injections are performed by administering a drug mixture containing dalargin 1 mg, lidocaine 40 mg, mexidol 50 mg, gemaza 5000 ME. The course of treatment is 6-7 injections daily. In addition, an intravenous injection of ozonized saline with an ozone concentration of 400-800 mcg/l, volume of 150-200 ml, at a rate of 30-40 drops per minute, with a course of 6-7 injections, is performed daily.EFFECT: persistent long-term clinical outcome, consisting in improved visual functions, increased visual acuity due to relief of inflammation in the uveal tract.3 cl, 6 dwg, 3 ex
ethod for treatment of tear-hemorrhagic syndrome (mallory-weiss esophageal tear) // 2633925
FIELD: medicine.SUBSTANCE: endoscope is used for combined administration of a haemostatic agent and a granular sorbent, represented by powdered bile powder applied to the bleeding defect with an insufflator in the amount 0.2 g from a distance of 1.0 cm from the bleeding surface, and then I sephadex G-25 in the amount of 0.3 g is insufflated from a distance of 1.5 cm from the defect.EFFECT: method provides a multicomponent effect, which allows to reliably stop bleeding, reduce the frequency of hemorrhages recurrences, and shorten treatment duration.5 ex, 1 tbl
ethod for endoscopic treatment of ulcerous gastroduodenal bleeding // 2633588
FIELD: medicine.SUBSTANCE: combination of powdered haemostatic agents and a biologically active granular sorbent are used topically. First, powdered lyophilizate NovoSeven and zhelplastan are applied on the bleeding ulcerous defect with an insufflator, in equal quantities of 0.1 g each, from a distance of 1.0 cm from the bleeding defect, and then 0.3 g of diovin is insufflated from a distance of 1.5 cm from the ulcerous surface.EFFECT: method allows to increase hemostasis reliability and reduce the time of treatment.1 tbl
ethod for endoscopic stop of gastrointestinal bleeding // 2632771
FIELD: medicine.SUBSTANCE: activedrainage (BAA) sorbent is applied to the bleeding defect with an activated platelet-enriched autoplasm of the patient. Aniline is used as the sorbent BAA. And first, a pneumo-insufflator is used to apply powdered aniline to the bleeding defect in an amount of 0.3-0.5 g from a distance of 1.5-2 cm from the defect. Then, the activated platelet-enriched autoplasma of the patient in an amount of 0.7-1.0 ml is applied from a distance of 1.0-1.5 cm to the defect covered with granulated BAA sorbent, through the endoscopic catheter with a syringe. To obtain platelet-enriched plasma, the blood is divided into three fractions by centrifugation at 1800 rpm for 8 minutes and the middle fraction is selected. Activation of platelet-enriched plasma is performed before it is applied to the defect by adding a solution containing 10 ml of 10% calcium chloride solution and 500 units of thrombin.EFFECT: strengthening and increasing the reliability of hemostasis, reduced risk of bleeding recurrence, reduced duration of treatment for stomach and duodenum defects, reduced number of emergency operations at the height of bleeding.3 cl, 1 tbl, 4 ex
ethod for correcting age-related changes in face and/or body soft tissues // 2629527
FIELD: medicine.SUBSTANCE: for correction, a course of three procedures is performed, including the introduction of thrombocytic autoplasm (TAP) into tissues. Before each procedure, sampling of blood in a volume of 9-36 ml is performed, which is centrifuged to obtain a native form of TAP. In carrying out the first two procedures 4 ml of the obtained native TAP form are taken into 4 sterile syringes by 1 ml in each syringe and placed in a thermostat at a temperature of 56°C and exposure for 5 minutes to obtain a fibrin form of TAP. The fibrin TAP form is mixed with 4 ml of native TAP form through the connector, connecting each syringe with the fibrin TAP form to the syringe with the native TAP form and making 5-10 passes from one syringe to the other. The resulting mixture is introduced into the upper layer of the dermis with a needle with a diametre of 30G and a size of 0.3×12 mm or with a cannula with a diametre of 2×5G and a length of 50 mm by papular, tubercular, linear-retrograde, fanning or bolus techniques of mesotherapy. When carrying out the third procedure 4 ml of the native TAP form is taken into 4 sterile syringes by 1 ml in each syringe and placed in a thermostat at a temperature of 75-80°C and exposure for 10 minutes to obtain a protein form of TAP. After cooling for 2 minutes, syringes with the protein TAP form are connected by a connector with an empty syringe and 10-15 passes are made from the syringe with a protein form into each empty syringe and back. The mixture is introduced into the deep layers of the dermis, the hypodermis and the periosteum by means of a cannula with a diametre of 22G and a length of 57 mm by linear-retrograde, fanning or bolus technique. If necessary, the course is repeated 2 weeks after.EFFECT: increased efficiency.4 cl, 2 ex
ethod for biofidobacterium metabolism products obtaining for treatment of combined radiation-thermal body damage and method for treatment of combined radiation-thermal body damage // 2627669
FIELD: medicine.SUBSTANCE: bifidobacteria are grown in 400-500 cm3 flasks with Blaurokk medium for 3-4 days in order to obtain products of their metabolism. The concentration of microbes is determined with standardization of their number to 1×108 CFU/cm. The bifidobacteria mixture with the products of their metabolism in the culture liquid is carefully shaken, poured into 50-100 cm3 centrifuge tube, centrifuged at 5000 rpm for 30 minutes. The resulting supernatant is decanted, the dry matter content is determined and adjusted to 10-15 mg/ml with physiological saline, sterilised by filtration, poured into bottles and stored in a refrigerator at a temperature of 4-6°C. Treatment of combined radiation-thermal body damage includes a 3-fold subcutaneous administration of the anti-ray serum of mammals mixed with metabolic products of bifidobacteria contained in the culture fluid obtained during the bifidobacteria growth in the ratio of 0.5:0.5 at a dose of 18.5-28 mg/kg in terms of protein, with an interval of 24, 48 and 168 hours after radiation-thermal trauma. Then, after 24, 48 and 168 hours, an ointment based on bee products and herbaceous oil is applied to the affected area.EFFECT: increased effectiveness of treatment of radiation-thermal body damage.2 cl, 4 tbl, 4 ex
ethod for treatment of destructive chronic periodontitis using injection form of autological platelet plasma // 2627582
FIELD: medicine.SUBSTANCE: method includes instrumental processing using rotor instruments with an extension of the apical part of the channel to a diameter of at least 025 ar per ISO, conicity of at least 4%, drug treatment of the dental canals with passive ultrasonic irrigation and canal drying, temporary obturation of canals with calcium hydroxide with sealing under glass ionomer cement, general drug therapy. Drug treatment is carried out with a 17% solution of ethylenediaminetetraacetate and 1.5% sodium hypochlorite solution, after 12-24 hours after calcium hydroxide repfreshing, injections of platelet autoplasma (PAP) are made into soft tissues in the fistula area, if present, submucosally in the transitional fold, subperiosteally in the projection of the apexes involved in the pathological process of roots from the vestibular or palatal, lingual surfaces. At that, calcium hydroxide refreshing and PAP injection the root canals are carried out once or 2-3 times with an interval of 7-10 days, then canal obturation is effected by lateral or vertical condensation of gutta-percha and the epoxide-amine resin-based sealer.EFFECT: use of the invention provides relief of acute inflammatory process, reduction of treatment time, stimulation of bone tissue regeneration and replacement of a defect in a chronic inflammation focus by accelerating natural mechanisms of tissue regeneration due to platelet-derived growth factors.6 dwg, 2 ex
ethod for atrophic skin scars treatment // 2627468
FIELD: medicine.SUBSTANCE: fully straightened filtered radio waves of high frequency, obtained by the radio wave device Surgitron®, and an autologous platelet-rich plasma are used. At that, blood is taken from the peripheral vein of the patient into vacuum tubes. Blood is mixed with an anticoagulant. The blood mixture is then centrifuged with an anticoagulant for 4-6 minutes at a centrifuge rotation speed of 700-900 rpm. After that, the upper layer of the platelet-rich plasma is extracted into a separate tube and activated by calcium ions by addition of calcium gluconate. The activated platelet-rich plasma is placed into the syringe and injected intradermally into the scar. The injected region is then warmed up to 41-43°C by the Surgitron® radio wave device with Pelleve® ClideSafe electrodes or PelleFirm for at least 4 minutes.EFFECT: invention allows to shorten the time of scars treatment in patients.11 cl, 1 ex
ethod for hypertrophic gingivitis treatment in patients with non-removable orthodontic devices with application of injection form of autological platelet-rich plasma // 2626826
FIELD: medicine.SUBSTANCE: for conservative treatment of hypertrophic gingivitis in patients with non-removable orthodontic devices against the background of professional oral hygiene and topical application of chlorhexidine within 10 days, fraction of platelet-rich autologous plasma obtained from the supernatant space of tube after centrifugation is injected intrapapillary into the hypertrophic gingiva papillae, and the remaining fraction is introduced submucosally into the transitional fold in a total volume of 8-9 ml in the area of all teeth. The injection procedure is repeated after 7 days.EFFECT: method allows to shorten the treatment time, to stop the inflammatory process, to stimulate regeneration due to acceleration of its natural mechanisms due to the growth factors contained in platelets in the prevention of relapse.5 dwg, 1 tbl, 2 ex
ethod for prevention of periodontium inflammatory diseases in patients with non-removable orthodontic devices with application of injection form of autological platelet-rich plasma // 2626825
FIELD: medicine.SUBSTANCE: for prevention of inflammatory periodontal diseases in patients on orthodontic treatment with non-removable devices after the hardware professional oral cavity hygiene with local use of chlorhexidine for 7 days, autologous platelet plasma is injected submucosally into the transitional fold in a total volume of 8-9 ml in the region of all teeth. The procedure is repeated in the framework of supporting periodontal therapy at intervals of 4-6 months during orthodontic treatment and once in the retention period.EFFECT: method allows to prevent inflammatory complications from the periodontal tissues due to the acceleration of the natural tissue regeneration mechanisms.4 dwg, 1 tbl, 1 ex
ethod of treatment of posttraummatic fibrosive ankiloses of small juices of brush // 2626591
FIELD: medicine.SUBSTANCE: method includes the installation of an external fixation device (EFD) and the implementation of a stage distraction of the joint area up to 9 mm by 3 mm per day. In the formed cavity on the 3rd and 6th day after the AVF installation, 1.5-2 ml of plasma enriched with platelets (PRP) is injected, which amounts to 1,000,000-1200,000 cells. After the second injection, the EFD is dismantled and active rehabilitation treatment is carried out. The third injection of PRP is performed pararticularly on the 9th day.EFFECT: reducing the time to restore or improve the function of the fingers.1 ex, 5 dwg
ethod for adhesiogenesis stimulation in pleural cavity in case of polytraumas with predominant thoracic involvement // 2625002
FIELD: medicine.SUBSTANCE: invention concerns adhesionogenesis stimulation in the pleural cavity in case of polytraumas with predominant thoracic involvement. To do this, a 10% homogenized suspension of autologous adipose tissue in a physiological solution is injected pointwise into the pleural cavity into the ribs fracture region subpleurally, under the parietal pleura.EFFECT: method ensures pulmonary framework stabilisation and early fusion of ribs due to adhesiogenesis stimulation and adhesions formation in the pleural cavity.2 cl, 1 ex
ethod for treatment of patients with acute stage of diabetic neurosteoartropathy of charcot foot type // 2624230
FIELD: medicine.SUBSTANCE: invention relates to treatment of the acute stage of diabetic neuro-osteoarthropathy ("Charcot foot"). To do this, the affected limb is unloaded and 2.0 to 4.0 ml of platelet-enriched autoplasma is injected into the area of aseptic inflammation and osteodestruction. The number of procedures is from 1 to 3 per a course of treatment of up to 2-3 weeks.EFFECT: method prevents the development of destruction of the foot osteoarticular apparatus, deformation and plantar ulcer defects formation.1 ex
acular rupture surgery technique // 2620929
FIELD: medicine.SUBSTANCE: method involves vitrectomy with excision and excision of the posterior hyaloid membrane. The previously stained internal border membrane is also removed. Active and (after 5-7 minutes) additional passive aspiration of the vitreous remains over the rupture is performed without mechanical reduction of the rupture edges. Platelet-rich blood plasma (PRP) is introduced, and final retinal tamponade is performed with air. At that, the macular rupture area is treated with sulfated glycosaminoglycans at a concentration of 1 to 5% prior to PRP introduction and at a concentration of 0.05% after PRP introduction.EFFECT: improved visual functions and reduced trauma of the corneal shell.2 ex
ethod for treatment of patients with sclerotic vulvar lichen // 2620683
FIELD: medicine.SUBSTANCE: method involves application of a complex therapy with the diagnosed sclerotic lichen, using autologous cellular regeneration by autoplasma enriched with platelets in combination with an antiviral drug and low-intensity laser light (LILI).EFFECT: use of the invention allows to reduce itching and dystrophic processes in the vulva area, accompanied by inflammatory manifestations in the dermis, fragmentation and disintegration of the elastic and nerve fibers, microcirculatory disturbances that characterize the sclerotic lichen, with formation of remission and prevention of disease progression into oncopathology.1 tbl
ethod for knee joint osteoarthrosis treatment // 2620495
FIELD: medicine.SUBSTANCE: injection of platelet-rich of autoplasma in an amount of 2 ml at the first injection into the aseptic necrosis zone and/or the cystic cavity located in the subchondral zone, using a needle, via the medial condyle of the tibia and/or the medial condyle of the femur, 2-3 times per treatment course with an interval of 7-10 days. For each subsequent injection, the volume of platelet-rich autoplasm is 2 times greater than for the previous injection.EFFECT: method allows to ensure low invasiveness of intervention, to shorten the pain relief period, to quickly restore the structure of the affected bone area, does not require the patient to stay in hospital, significantly improves the results of treatment of patients with this pathology, shortens the rehabilitation period and improves the quality of life.4 cl, 1 ex
ethod for treatment of degenerative-dystrophic diseases and locomotor system injuries consequences // 2618928
FIELD: medicine.SUBSTANCE: for treatment of degenerative-dystrophic diseases and locomotor system injuries consequences, autoplasma enriched in platelets is preliminarily prepared, in a volume of 2.0-6.0 ml. A composition of glucosamine sulphate and chondroitin sulphate, in a volume of 3.0 and 4.0 ml, respectively, is administered intra-articularly and/or to periarticular trigger points in the pathological portion. Immediately thereafter, the prepared autoplasma is slowly introduced into the same portions. A sterile retainer is glued on the needle puncture points region. Few minutes later, an extracorporeal shock wave therapy (ESWT) session is performed excluding any expressed discomfort during the session. In the days of procedures, the patient adhers to a conservative orthopedic regime. Procedures are carried out every 10-12 days, a course of 2-6 times.EFFECT: reduced number of relapses and increased period of remission, including by accelerating the regeneration processes, eliminated risk of post-injection side effects at minimally invasive surgery.3 tbl, 2 ex

ethod, test tube and device for making of compositions for wound healing // 2614722
FIELD: medicine; pharmaceuticals.SUBSTANCE: group of inventions relates to pharmaceutics and medicine and concerns method of making composition for healing of wounds and tissues including sampling of whole blood in test tube containing hyaluronic acid without mixing of whole blood and hyaluronic acid, centrifugation of said test tube and collection of supernatant containing said hyaluronic acid and platelet rich plasma. Composition obtained using said methods is used as matrix, cell nutrient medium or medium for implantation of cells and can be used in surgery, dentistry, orthopedics, mesotherapy, as well as in cosmetics and/or esthetics.EFFECT: group of inventions provides immediate use of obtained autologous blood cells with simultaneous preservation of their integrity for wound healing.25 cl, 14 ex, 16 dwg
Combination for acne treatment and method of its application // 2614392
FIELD: pharmacy.SUBSTANCE: combination for acne treatment, comprising an aqueous Gynura Procumbens leaf extract, and means containing non-activated platelets and white blood cells in their own blood plasma. A method of acne treatment by application of the above combination.EFFECT: combination and treatment method are characterized by high efficiency in acne treatment.2 cl, 2 dwg

Treatment method for chronical atrophic pharyngitis // 2613186
FIELD: medicine, pharmaceutics.SUBSTANCE: invention refers to the medicine, particularly, to the otolaryngology, and may be used for the treatment of the chronical atrophic pharyngitis. For this purpose, an application anesthesia with 2% lidocaine solution is performed, autoplasma containing thrombocytes with the concentration of (351-451)109/l is administered into the submucous membrane of the pharynx, one 0.2-0.3 ml injection to the area of each lateral cushion, and two injections of 0.2-0.3 ml are administered either into each upper part or into each lower part of the pharynx. The procedures with 6 injections are performed in 4-6 days, whereupon, the luminotherapy is additionally conducted with the impact on the oropharynx with an open mouth, and/or on the left and right sides of the neck by the non-coherent, polarized, polychromatic, and low-energy light with the wave-length of 480-3,400 nm, flow density of the light energy per minute of 2.4 J/cm2, the degree of polarization of 95-98% during 10-12 minutes daily with 12-14 days.EFFECT: method of actuation of the regeneration process of the mucous membrane of the pharynx and improvement of microcirculation in tissues without side effects.3 dwg, 2 ex
ethod for treating chronic inflammation of paradentium tissues and mucous membrane with hyperkeratosis in young individuals // 2606040
FIELD: medicine.SUBSTANCE: invention relates to medicine, namely to therapeutic dentistry. Proposed method of treating chronic inflammation in case of inflammatory periodontal diseases involves injection of platelet-rich plasma into gingival papilla, marginal part of the gum and transitory fold, wherein said injection is performed at the volume of 0.1–0.5 ml per mm2 – once a week, 2–3 times, Additionally, the placental preparation – "Laennek" – is injected in gingival papilla, marginal part of the gum and transitory fold at the volume of 0.1–0.2 ml per mm2, at 1 injection every 2 days, overall 5–10 injections.EFFECT: use of the invention increases efficiency of local treatment of chronic inflammation in case of inflammatory periodontal diseases – gingivitis and periodontitis, and allows to prolong remission.1 cl, 1 ex

ethod of treating septic shock accompanied with agranulocytosis // 2604672
FIELD: medicine.SUBSTANCE: invention refers to medicine, namely to intensive therapy, and can be used for septic shock treating. This is ensured by intravenous introduction of multipotent mesenchymal stem cells derived from the donor's bone marrow, grown on a medium with human blood plasma enriched with lyzed thrombocytes in amount of 1-1.5×106 of the transplanted cells per 1 kg of patient's body weight once in the first 10 hours from the moment of septic shock developing.EFFECT: given method enables to use multipotent mesenchymal stem cells, reducing the intensity of multiple organ failure and thereby improving therapeutic effects of septic shock treatment accompanied with agranulocytosis.1 cl, 3 ex, 2 dwg
ethod for esthetic remodelling of involutional changes of facial skin // 2603618
FIELD: medicine.SUBSTANCE: invention refers to medicine, namely to physiotherapy and dermatocosmetology, and can be used for esthetic remodelling of involutional changes of facial skin. Single-step infrared low-intensity laser radiation and platelet rich blood plasma impacts are applied. Intervention is performed in two stages: at the first stage patient's venous blood sampling is performed in the volume of 20-40 ml, and it is centrifuged at 4,000 rpm for 5 minutes to obtain the fraction of platelet-rich plasma. At the second stage plasma is applied on facial skin and without time interval infrared low-intensity laser radiation impact with the wave length of 0.89 microns is applied. Pulse repetition frequency is from 80 to 1,500 Hz - mode of constantly varying frequency. Pulse power is 4-6 W/pulse. Contact-labile technique, 1-3 minutes per a field. Total exposure time is up to 10 minutes. For the therapeutic course of 10 procedures 2 times a week.EFFECT: method provides increase in effectiveness of involutional changes reduction of facial skin, improves quality characteristics of skin, ensure decrease of hyperpigmentation and elimination of telangiectasia by trophic and regenerative effects, normalization of water and oxygen metabolism in tissues, increase in production of collagen and elastin by fibroblasts.1 cl, 5 dwg, 2 ex
ethod of treating patients with severe ovary hyperstimulation syndrome // 2603319
FIELD: medicine.SUBSTANCE: invention relates to medicine, specifically to gynaecology. Treatment consists of two stages. First stage is carried out before entry into the therapeutic cycle and involves sampling, freezing and storage of autoplasma, herewith within the period from 12 months to 30 days before entry of the patient into the therapeutic cycle. 3-4 plasmapheresis sessions are performed. After exfusion the blood taken from the median cubital vein is centrifuged in a cold centrifuge. Corpuscles are returned to the patient, and the obtained autoplasma is frozen at the temperature of -40 °C, herewith the volume of the taken blood per one session of plasmapheresis is 250-300 ml in patients with the weight up to 55 kg and 400-500 ml in patients with the weight over 55 kg, with an interval between sessions of not less than 7 days. Second stage is used as per the fact of diagnostics of the ovary hyperstimulation syndrome, previously prepared frozen autoplasma is thawed and its infusion is done not later than 1 hour after the thawing using a sterile infusion system for infusion solutions with a filter for 170-200 mcm and an air valve.EFFECT: method enables preventing developing multiple organ failure by restoring the volume of circulating plasma; elimination of hemoconcentration, electrolyte imbalance, prevention of acute renal insufficiency and thromboembolic complications, reducing the length of treatment of ovary hyperstimulation syndrome and reducing the rate of adverse reactions.1 cl, 5 ex

ethod of treating endothelial epithelial corneal dystrophy // 2601317
FIELD: medicine.SUBSTANCE: invention relates to medicine, namely to ophthalmology, and aims at treating endothelial epithelial corneal dystrophy (EECD). Method involves introducing a suspension of autologous blood mononuclear leukocytes into the posterior chamber of the eye until it replaces the aqueous humor in the anterior chamber by performing paracentesis near the corneal limbus. For this purpose, paracentesis is performed twice at 7 and 11 o'clock with the puncture size of 1-2 mm, wherein an irrigation system in the form of a cannula used to supply sterile air at the pressure of up to 0.2 atm into the anterior chamber is installed into one of the punctures; and 96-98 % cell suspension of autologous blood mononuclear leukocytes is syringed once through the second paracentesis into the anterior chamber between the inner surface of the cornea and air bubble. Then the irrigation system is removed and edges of paracenteses are hydrated. Suspension of autologous blood mononuclear leukocytes is obtained by extraction using the density gradient fractionation method and separating solution of Ficoll-verografin.EFFECT: use of the invention enables to reduce the length of treatment and complications associated with excessive involvement of ocular tissues, corneal oedema.1 cl, 10 dwg
ethod of treatment of chronic odontogenic osteomyelitis in drug-addicts patients // 2599874
FIELD: medicine.SUBSTANCE: invention refers to medicine, namely to maxillofacial surgery, and can be used during the final stage of operation sequestrectomy in chronic Odontogenic osteomyelitis in drug-addicts patients. Note here that before the operation is prepared thrombocyte enriched blood plasma (PRP-gel), for this purpose native blood is sampled from the patient's vein in a volume of 40-80 ml. Then injected into the blood anticoagulant, then platelet enriched blood plasma by centrifugation. Besides, before the surgery, also prepared blood plasma, rich with thrombocytes with high content of fibrin (FRP-membrane), for this purpose native blood is sampled from the patient's vein in a volume of 40-80 ml, activator is administered in blood, then enrich with platelets by centrifugation. During the operation sequestrectomy injection of PRP-gel in the spongy substance of wound edges of the bone defect jaw is carried out, a bone defect is filled and closed by FRP-membranes. After that provided mobilization and movement of the mucoperiosteal flap with closing the bone defect and the wound is closed tightly.EFFECT: method ensures high efficiency of operation with low traumatism and low cost.6 cl
ethod of treating osgood-schlatter syndrome // 2599202
FIELD: medicine.SUBSTANCE: invention relates to medicine, namely to orthopedics, and can be used for the treatment of an Osgood-Shlatter disease. To do this, in the area of the affected apophis from medial, lateral, distal and proximal sides of the tibial tuberosity, as well as from front to back in the region of maximum pain, by injection administer platelet rich autoplasma. Thus autoplasma is obtained in 10-15 minutes before the procedure and is administered in equal portions in a total dose from 2 to 3 ml.EFFECT: method provides a low-trauma intervention, reducing the period of pain relief, acceleration of time of clinical and radiological recovery with no recurrence of the disease due to the effective stimulation of bone tissue regeneration in the knee joint as a result of revascularization, improvement of microcirculation and acceleration of metabolism of the tissues.1 cl, 2 ex

ethod of treatment skin wounds in mammals with the help of thrombocytic auto plasma // 2599126
FIELD: veterinary science.SUBSTANCE: invention refers to treating skin wounds of mammals. Method involves blood sampling in a mammal and preparation of the required volume of thrombocytic auto plasma. Wherein previously carry out the measurement of wound area. For vast wounds with area more than 20 cm2 determination of the injected volume of thrombocytic auto plasma is carried out in the following way: wound diameter is measured in the widest part thereof and prepare volume of thrombocytic auto plasma in ml, which is equal to diameter of the wound in cm. Obtained volume of thrombocytic auto plasma is divided into 4-5 injections, which is carried out at the boundary of the wound surface with the healthy skin. Needle is inserted at a depth of 0.5-1 cm. So the wound is treated with 1 time of 3, 5 or 7 days. Frequency of administration is 3 to 6 times depending on the status of the wound. Administration of thrombocytic auto plasma is terminated after completion of the stage of epithelization.EFFECT: method enables to increase effectivity of wound repair in any type of a mammal, promotes faster healing process, as well as prevention and treatment of purulent process in the wound.1 cl, 5 dwg, 1 ex
ethod of ossiculoplasty // 2597369
FIELD: medicine.SUBSTANCE: invention relates to medicine, particularly to otorhinolaryngology. It takes place the replacement of pathologically altered fragments of auditory ossicles with bioinert transplantate and covering the bottom of the translantate with platelet-rich pieces of autoblood plasma of a patient, sized 2-3 mm. Plasma pieces are strengthened by application of several drops of bioadhesive “Sulphacrylate” with further waiting during 1-2 minutes for bioadhesive to demonstrate its fixative power.EFFECT: method allows simplifying attachment of prosthetic elements, reducing risk of their displacement during postoperative period till formation of physiological fixation by creation simple and efficient biodegradable frame, which holds prosthesis in required position without aggressive impact on the structures of middle and inner ears.1 cl, 1 ex
ethod of conservative treatment of trophic ulcers of soft tissues // 2593582
FIELD: medicine. SUBSTANCE: invention refers to medicine, namely to surgery, and can be used for conservative treatment of patients with trophic ulcers of soft tissues. That is preceded by activation of autoplasma by centrifugation for 5 minutes with subsequent addition of 10 % calcium chloride solution in amount of 1:1. Then mixture is controlled for 20 minutes at temperature of 40 °C to obtain stabilised biologically active film. After treatment of ulcer and adjoining tissue with shock wave 100-1,000 strikes per cm2, with impact strength 0.1 mJ/1 mm2 and treatment of ulcer with sterile neutral solution produced film is applied on ulcer and covered with aseptic dressing. Procedure is repeated 7-8 times every 5 days. EFFECT: method allows achieving healing trophic ulcers, reduced risk of extensive intermuscular hematomas, bleeding wound infections, thromboembolism, does not require hospital patients and anaesthesia care. 1 cl, 1 ex

ethod and apparatus for producing serum with thrombin from one donor // 2589648
FIELD: medicine.SUBSTANCE: group of inventions relates to transfusion medicine. Serums with thrombin are produced. For this purpose liquid blood sample is obtained, having first aliquot of liquid blood with procoagulant to make prothrombinaze enzyme complex associated with surface of procoagulant agent, which enables to obtain activated procoagulant agent, which can be stored. Activated procoagulant agent then may be subjected to interaction with second liquid blood aliquot containing prothrombin to obtain serum with Thrombin, which can be extracted and subjected to interaction with fibrinogen for preparing fibrin. To obtain serum with Thrombin special device is used containing reaction chamber with one or several compartments to hold procoagulant agent and liquid part of blood without flow. Procoagulant agent can reaction with said liquid part of blood to form fibrin gel, appliances to feed fluid into and from reaction chamber, means to retain solid particles in reaction chamber; agent for serum separation with Thrombin from fibrin gel. Further, device also comprises ventilation hole made with possibility of avoiding long period of staying under excessive pressure of reaction chamber. At that, one or several solid floating objects are present in reaction chamber, wherein solid loose objects, have diameter within range of 50 mcm and 2 mm, and other solid loose objects are characterised by dimensions selected for grinding of fibrin gel, and in cross section have, at least 4 mm.EFFECT: group of inventions allows eliminating risk of toxic reaction in patient in response to introduction of serum with Thrombin, risk of immunogenic reactions and risk of infectious diseases transmission from donors.25 cl, 8 dwg, 2 tbl

Preparation for prevention and treatment of diarrhoea in calves // 2583555
FIELD: veterinary science.SUBSTANCE: invention can be used for prevention and treatment of diarrhoea in calves. That is ensured by preparation containing hyperimmune polyvalent blood serum against diarrhoea with antibody titre of 1:300-1:600 in effective amount of sodium hypochlorite solution concentrated 300-350 mg/l at ratio of ingredients, wt%: serum: sodium hypochlorite 70:30-60:40, preserving agent, wherein preserving agent used is sodium hypochlorite solution in concentration of 700-750 mg/l in amount of 10-11 % of preparation.EFFECT: invention reduces cost of preparation and improves environmental safety of use while maintaining high efficiency and absence of side effects.1 cl, 4 tbl
ethod for soft tissue augmentation by means of heat-processed platelet-rich autoplasma // 2582948
FIELD: medicine.SUBSTANCE: method for face and/or neck soft tissue augmentation involving venous blood sampling in an amount of 9-36 ml; thereafter, the blood is centrifuged with acceleration of 1000 G for 5 minutes; the produced platelet-rich autoplasma is taken from the test tube in an amount of 3.0 ml by means of luer-lock syringes. The syringes containing the platelet-rich autoplasma are placed into a thermostat and kept there to produce gel. To introduce into a periorbital and neck region, the platelet-rich autoplasma is kept at 45°C for 5 minutes, or at 85°C for 6 minutes if it is necessary to introduce it into the other face regions. The produced gel is cooled down in a sterile tray for 2 minutes. By means of a connector, the syringe with the produced gel is attached to an empty sterile syringe; the produced gel is transited from one syringe into the other through 5-10 passages and introduced into the face region, avoiding the periorbital region, by means of a short-bevel needle 21G×3.8 cm. Before introduced into the periorbital and/or neck region, the produced gel of the platelet-rich autoplasma is mixed with untreated and platelet non-enriched autoplasma taken from the same patient, in ratio 2:1 and introduced into the periorbital and/or neck tissues by means of a short-bevel needle 27G×13 mm.EFFECT: method provides ensuring the uniform distribution and adequate correction of the desired regions.3 cl, 1 ex

ethod of treating acute anal fissure and persistent wounds of anal canal // 2578853
FIELD: medicine.SUBSTANCE: in treating acute anal fissure is auto plasma bubbling with increased content of platelets by ozone-oxygen mixture. Transferred by heating it in gel form, is introduced under anal crack in amount of 2.5-3.0 ml.EFFECT: method improves the anal fissure epithelization and postoperative rehabilitation due to prolonged therapeutic effect, minimum operation.1 cl, 1 ex, 4 dwg

ethod of treatment of osteoarthrosis of animals // 2565413
FIELD: veterinary science.SUBSTANCE: method includes effecting of the affected joint by enriched with platelets autoplazma, prepared 15-20 minutes before procedure by taking of 10-15 ml of autoblood, its one-stage centrifugation at the speed 1000 rpm within 10 minutes, with preparation of 0.5-1 ml of autoplazma enriched with platelets with concentration of platelets from 1000 to 1200 thousand/mcl, activation of the prepared autoplazma by 10% CaCl2 solution in the ratio 1:10. Autoplazma is injected into a joint cavity at the angle 90°. The number of procedures per course of treatment amounts 3-4 injections with the interval 7-10 days.EFFECT: method is highly effective for treatment of animals with osteoarthrosis of knee and other joints.5 dwg, 2 ex

ethod for producing agent possessing cytostatic action on human lymphoblasts // 2563170
FIELD: medicine.SUBSTANCE: method for producing an agent possessing cytostatic action on human lymphoblasts, wherein chicken blood sampling is preceded by electrical stimulation of chickens' heads at 100-120 V, 3-5 A, for 3-5 seconds; that is followed by sampling and incubating the blood, recovering serum and peptide fraction from the serum at molecular weight 850-1200 Da to be lyophilised and sterilised by exposing in a linear electron accelerator in a dose of 28-32 kGy.EFFECT: method enables producing an agent, which effectively suppresses malignant cells in acute lymphocytic leukaemia in the patient's body, reduces haematological and immunological toxicity and a risk of severe complications observed in anti-cancer chemotherapy.3 dwg, 2 tbl

ethod of obtaining, at least, one compound from blood and device for extraction for application in realisation thereof // 2560843
FIELD: medicine, pharmaceutics.SUBSTANCE: invention relates to the pharmaceutical industry, in particular to a method of division of blood into fractions. The method of dividing blood into fractions consists in the division of blood into the fractions with the application of an extraction device of a certain construction under certain conditions. The extraction device for the division of blood fractions contains a body (1); the first needle (2) and the second needle (4), placed in selective flowing communication, where the said first needle (2) projects from the body (1); a switching device (3) for opening and closing flowing communication between the said needles (2, 4) and a button (3a) for bringing the said switching device (3) in action.EFFECT: method makes it possible to divide blood into fractions without mixing the said fractions.16 cl, 4 dwg, 5 ex

ethod of treating acute anal fissure // 2558995
FIELD: medicine.SUBSTANCE: treating acute anal fissure involves introducing autoplasma in an amount of 2-2.5 ml under the fissure. That is preceded by 5-minute autoplasma barbotage with ozone-oxygen mixture.EFFECT: method provides epithelising the anal fissure with minimum intraoperative injuries.3 dwg, 1 ex
ethod of obtaining fibrinogen concentrate // 2556804
FIELD: chemistry.SUBSTANCE: invention relates to biotechnology of obtaining haemostatic medications. Claimed is method of separating purified fibrinogen concentrate, free of viruses and ballast proteins. Solubilisation of cryoprecipitate of fresh frozen human plasma is realised. Fibrinogen is precipitated with 20-30% PEG solution. Separated sediment is dissolved in buffer with sodium citrate and sodium chloride. Virus inactivation of solution by solvent-detergent method is carried out in presence of 1-3% Tween-80 and 0.1-1.5% of tri-n-butylphoshate. Obtained concentrate is purified from products of virus inactivation and solvent-detergents by triple extraction with liquid paraffin. After that, obtained fibrinogen concentrate is re-precipitated with 1.0-2.5 M glycine solution. Sterile filtration and lyophilic drying with further corking of lyophilisate under vacuum and thermal inactivation are performed.EFFECT: invention makes it possible to obtained lyophilised form of fibrinogen concentrate with approximately 55% output.

Activated leukocyte composition // 2548741
FIELD: medicine, pharmaceutics.SUBSTANCE: group of inventions refers to medicine and concerns a method for making an activated leukocyte composition involving human leukocyte incubation, exposure to hypotonic shock and addition of physiologically acceptable saline in an amount sufficient for the reconstitution of isotonicity, to the leukocytes. The group of inventions also concerns using: the activated leukocyte composition in preparing a drug for wound healing; a wound healing dressing containing the above composition.EFFECT: group of inventions provides preparing the composition containing a 90 times increase of the leukocyte count as compared to the known state-of-the-art methods.16 cl, 3 dwg, 6 tbl, 3 ex
ethod of treating bullous keratopathy // 2544306
FIELD: medicine.SUBSTANCE: invention refers to medicine, particularly to ophthalmopathy, and can be used in treating endothelial-epithelial corneal dystrophy. That is ensured by de-epithelisation, 12-o'clock corneal incision and splitting, administration of a biologically active substance into a stromal pocket of the cornea. The biologically active substance is poludan-activated autoplasma. Before the epithelisation is completed, a soft contact lens is placed on the cornea. The operation is followed by instillations of ciprofloxacin, diclofenac 6 times a day and infusions of cornegel.EFFECT: method provides reducing the intraoperative injuries and the length of patients' treatment, including by local (intrastromal) autocytokinotherapy.2 ex
ethod of treating trophic ulcers // 2543344
FIELD: medicine.SUBSTANCE: treating trophic ulcers is ensured by using autoserum prepared of blood 20.0-25.0 ml; centrifuging and separating it from a protein-thrombocyte clot are followed by edge chipping under a wound bed, while the protein-thrombocyte clot is used to be laid on the wound directly. The above procedures are repeated up to 20 times every two days.EFFECT: invention enables providing fast wound healing with the adequate recovery of the dermal skin layer by using the high-biocompatibility material containing the ingredients creating the optimum medium for the wound regeneration.2 ex
ethod for prevention of diffuse intra- and postoperative blood loss in surgery of abdominal aorta // 2540923
FIELD: medicine.SUBSTANCE: invention refers to medicine, namely to cardiovascular surgery, and concerns preventing a diffuse intra- and postoperative blood loss in surgery of abdominal aorta. To this effect, performing the aortoiliac operations is combined with measuring preoperative antithrombin III; if the measured value is less than 87±3%, a thromboelastography is performed from the moment of an anaesthetic support started with determining a coagulation index. If the coagulation index is less than 2.89±0.25 as early as at the start of the stage of approaching the abdominal aorta, before any surgically significant blood loss, fresh-frozen plasma of the identical group in a min. amount of 600 ml is administered into the patient. If the following dynamic control shows a decrease of the coagulation index below 1.10±0.28, at least 600 ml of fresh-frozen plasma is additionally administered, providing thereby the coagulation index by the end of the operation not less than 0.52±0.27.EFFECT: method provides effective prevention of intra- and postoperative blood loss by prevention of intraoperative homeostatic disorders.
ethod for conservative management of plantar fasciitis // 2531946
FIELD: medicine.SUBSTANCE: invention refers to medicine, namely to traumatology and orthopaedics, and aims at conservative treatment of plantar fasciitis. That is ensured by administering platelet rich autoplasma in an amount of 2.0 ml for each injection into an attachment point of the plantar aponeurosis to the calcaneum (the heel sac), inner and outer side surfaces of a midfoot, as well as to a plantar surface of the midfoot. The autoplasma is prepared 1-2 hours before the procedure, and it contains from 1243 thousand/mcl to 3029 thousand/mcl platelets. The normal autoplasma contains from 162 thousand/mcl to 358 thousand/mcl platelets. The autoplasma is activated with 0.25% CaCl2 in a ratio of 2:1 with added 5% sodium hydrocarbonate in a ratio of 1:20 to the prepared platelet rich plasma.EFFECT: above method enables providing pain management, recovering the extremity function, ensuring the stable clinical effect, and preventing complications.2 ex
ethod for recovering estrual cycling and fertility in cows suffering from hypo-ovarionism // 2531939
FIELD: medicine.SUBSTANCE: declared invention refers to veterinary science and aims at recovering the estrual cycling and fertility in cows suffering from hypo-ovarionism. The method involves single parenteral administration of the gonadotrophic preparation of a pregnant mare serum gonadotropin - folligon in a dose of 1,000 IU on the 7th day from the beginning of treatment with underlying pre-injections of 2.5% progesterone in a dose of 4 ml, on the 1st, 3rd and 5th days of the therapeutic course, 2% Sinestrol in a dose of 2 ml on the 1st and 3rd days, and Eleovit in a dose of 5 ml on the 1st and 7th day of treatment.EFFECT: declared invention provides normalising the hormonal-metabolic status and the recovered functional ovarian activity in the cows.2 tbl, 2 ex